Homology
BLAST of Lcy01g013470 vs. ExPASy Swiss-Prot
Match:
Q9LNU1 (CO(2)-response secreted protease OS=Arabidopsis thaliana OX=3702 GN=CRSP PE=2 SV=1)
HSP 1 Score: 636.3 bits (1640), Expect = 4.4e-181
Identity = 350/754 (46.42%), Postives = 475/754 (63.00%), Query Frame = 0
Query: 36 YVVYMGSGGSNYEEDDQTAAELDYLQLLSSVIPRKEKERGSRDVVHQYHNAFKGFSAMLT 95
Y+VYMGS S AA + Q+L + + +R + D++H Y + F GF+A LT
Sbjct: 36 YIVYMGSASS--------AANANRAQILINTM----FKRRANDLLHTYKHGFSGFAARLT 95
Query: 96 EEEASSLSGVDGIVSVFPDPTLQLHTTRSWDFLDSITGLRPPTPLPPPHSYLSSTDVIVG 155
EEA ++ G+VSVFPDP QLHTT SWDFL T ++ + PP + S D IVG
Sbjct: 96 AEEAKVIAKKPGVVSVFPDPHFQLHTTHSWDFLKYQTSVKVDSG-PPSSASDGSYDSIVG 155
Query: 156 VVDTGIWPESQSFNDEGLGEIPSKWKGVCMEAPDFKKSNCNRKLVGARYYNAIEPNGNDS 215
++DTGIWPES+SFND+ +G IPS+WKG CMEA DFK SNCNRK++GARYY P+ +
Sbjct: 156 ILDTGIWPESESFNDKDMGPIPSRWKGTCMEAKDFKSSNCNRKIIGARYYK--NPDDDSE 215
Query: 216 RVGMPKGTPRDLLGHGTHTSSIAAGARVPNASYFDLARGTARGGGTVSTRIASYKVCAGV 275
T RD++GHG+H SS AG+ V NASY+ +A GTA+ GG+ + RIA YKVC
Sbjct: 216 YY-----TTRDVIGHGSHVSSTIAGSAVENASYYGVASGTAK-GGSQNARIAMYKVCNPG 275
Query: 276 GCSGAAILKAIDDAVKDGVDIISISIGIGSPLFQSDYLN-DPIAIGAFHAQQMGVLVVCS 335
GC+G++IL A DDA+ DGVD++S+S +G+P + LN DPIAIGAFHA + G+LV+CS
Sbjct: 276 GCTGSSILAAFDDAIADGVDVLSLS--LGAPAYARIDLNTDPIAIGAFHAVEQGILVICS 335
Query: 336 GGNDGPDPNTVGNIAPWIFTVAASNIDRDFQSTVVLGNGKTFQGTAINLSNLTTSKTYPL 395
GNDGPD TV N APWI TVAA+ IDRDF+S VVLG K +G I+ SN++ S YPL
Sbjct: 336 AGNDGPDGGTVTNTAPWIMTVAANTIDRDFESDVVLGGNKVIKGEGIHFSNVSKSPVYPL 395
Query: 396 VFGKDAAAKFTPISEARNCYPGSLDRSKVAGKIVICTSDDFSTSRTIKELVVQDAKAMGL 455
+ GK A + AR C SLD+ KV GKIV+C + S + V+ G
Sbjct: 396 IHGKSAKSADASEGSARACDSDSLDQEKVKGKIVLCENVGGSYYASSARDEVKSKGGTGC 455
Query: 456 ILINEASKTVPLDSNIFPFTQIGNSEGLQILEYINSTKNPTATILRTVEVRRLKPAPMVA 515
+ +++ ++ V FP T I + E +I Y+NSTK+P ATIL T V + PAP VA
Sbjct: 456 VFVDDRTRAVASAYGSFPTTVIDSKEAAEIFSYLNSTKDPVATILPTATVEKFTPAPAVA 515
Query: 516 YFSSRGPSPLTENILKPDITAPGVSILAAVVPKSDAESGPIGKKPSDYAMRSGTSMACPH 575
YFSSRGPS LT +ILKPDITAPGVSILAA +D+ GK S Y + SGTSMA PH
Sbjct: 516 YFSSRGPSSLTRSILKPDITAPGVSILAAWT-GNDSSISLEGKPASQYNVISGTSMAAPH 575
Query: 576 VAGAAAFVKSVRHNWSSSMIKSALMTTATIYDNQRKFMRNSTNNPSNPHEMGAGEISPIK 635
V+ A+ +KS W S I+SA+MTTAT +N + + T + P++ GAGE+S
Sbjct: 576 VSAVASLIKSQHPTWGPSAIRSAIMTTATQTNNDKGLITTETGATATPYDSGAGELSSTA 635
Query: 636 ALNPGLVFESTNEDYLRFLCYYGYSNKIIRSVSK---QNFSCPKTSSEDLISSVNYPSIS 695
++ PGLV+E+T DYL FLCYYGY+ I+++SK +NF+CP S+ DLIS++NYPSI
Sbjct: 636 SMQPGLVYETTETDYLNFLCYYGYNVTTIKAMSKAFPENFTCPADSNLDLISTINYPSIG 695
Query: 696 IGKLDRKQAAKVIERTVTNVGAP-NTTYIAKVHSSEGLLVKVIPSKIVFSENVKKVTFKV 755
I + +K + RTVTNVG Y V + G ++V P K+ F+++ +K+T++V
Sbjct: 696 ISGF-KGNGSKTVTRTVTNVGEDGEAVYTVSVETPPGFNIQVTPEKLQFTKDGEKLTYQV 755
Query: 756 SFYGKEARSGYNFGTITWRDSVHSVRTVFAVNVE 785
+ FG +TW ++ + VR+ ++ E
Sbjct: 756 IVSATASLKQDVFGALTWSNAKYKVRSPIVISSE 764
BLAST of Lcy01g013470 vs. ExPASy Swiss-Prot
Match:
F4HSQ2 (Subtilisin-like protease SBT5.1 OS=Arabidopsis thaliana OX=3702 GN=SBT5.1 PE=3 SV=1)
HSP 1 Score: 563.9 bits (1452), Expect = 2.8e-159
Identity = 322/765 (42.09%), Postives = 470/765 (61.44%), Query Frame = 0
Query: 36 YVVYMGSGGSNYEEDDQTAAELDYLQLLSSVIPRKEKERGSRDVVHQYHNAFKGFSAMLT 95
Y++YMG+ S+ D+ D+++LLSS++ +R + +H+Y + F GF+A L+
Sbjct: 33 YIIYMGAASSDGSTDN------DHVELLSSLL-----QRSGKTPMHRYKHGFSGFAAHLS 92
Query: 96 EEEASSLSGVDGIVSVFPDPTLQLHTTRSWDFLDSITGLRPP--TPLP-PPHSYLSSTDV 155
E+EA ++ G++SVFPD LQLHTTRSWDFL + R T + S + D
Sbjct: 93 EDEAHLIAKQPGVLSVFPDQMLQLHTTRSWDFLVQESYQRDTYFTEMNYEQESEMHEGDT 152
Query: 156 IVGVVDTGIWPESQSFNDEGLGEIPSKWKGVCMEAPDFKKSN--CNRKLVGARYYNA--- 215
I+G +D+GIWPE+QSFND +G +P KWKG CM + + CNRKL+GARYYN+
Sbjct: 153 IIGFLDSGIWPEAQSFNDRHMGPVPEKWKGTCMRGKKTQPDSFRCNRKLIGARYYNSSFF 212
Query: 216 IEPNGNDSRVGMPKGTPRDLLGHGTHTSSIAAGARVPNASYFDLARGTARGGGTVSTRIA 275
++P+ TPRD LGHGTH +SIAAG + NASY+ LA G R GG+ S+RIA
Sbjct: 213 LDPDYE---------TPRDFLGHGTHVASIAAGQIIANASYYGLASGIMR-GGSPSSRIA 272
Query: 276 SYKVCAGVGCSGAAILKAIDDAVKDGVDIISISIGIGSPLFQSDYLNDPIAIGAFHAQQM 335
Y+ C+ +GC G++IL A DDA+ DGVD+ISIS+G L+ + L DP++IG+FHA +
Sbjct: 273 MYRACSLLGCRGSSILAAFDDAIADGVDVISISMG----LWPDNLLEDPLSIGSFHAVER 332
Query: 336 GVLVVCSGGNDGPDPNTVGNIAPWIFTVAASNIDRDFQSTVVLG--NGKTFQGTAINLSN 395
G+ VVCS GN GP +V N APW+ TVAAS IDR F+S ++LG + +G IN++N
Sbjct: 333 GITVVCSVGNSGPSSQSVFNAAPWMITVAASTIDRGFESNILLGGDENRLIEGFGINIAN 392
Query: 396 LTTSKTYPLVFGKDAAAKFTPISEARNCYPGSLDRSKVAGKIVICTSDDFSTSRTIKELV 455
+ ++ YPL+ + A ARNC P +LD++ V GKIV+C SD + K
Sbjct: 393 IDKTQAYPLIHARSAKKIDANEEAARNCAPDTLDQTIVKGKIVVCDSDLDNQVIQWKSDE 452
Query: 456 VQDAKAMGLILINEASKTVPLDSNIFPFTQIGNSEGLQILEYINSTKNPTATILRTVEVR 515
V+ +G++L+++ S + F T I +G+QI+ YINST+ P ATI+ T
Sbjct: 453 VKRLGGIGMVLVDDESMDLSFIDPSFLVTIIKPEDGIQIMSYINSTREPIATIMPTRSRT 512
Query: 516 RLKPAPMVAYFSSRGPSPLTENILKPDITAPGVSILAAVVPKSDAESGPIGKKPSDYAMR 575
AP + FSSRGP LT +ILKPDI APGV+ILA+ + D + P GK P + +
Sbjct: 513 GHMLAPSIPSFSSRGPYLLTRSILKPDIAAPGVNILASWL-VGDRNAAPEGKPPPLFNIE 572
Query: 576 SGTSMACPHVAGAAAFVKSVRHNWSSSMIKSALMTTATIYDNQRKFMRNSTNNPSNPHEM 635
SGTSM+CPHV+G AA +KS +WS + I+SA+MTTA N + T + P++
Sbjct: 573 SGTSMSCPHVSGIAARLKSRYPSWSPAAIRSAIMTTAVQMTNTGSHITTETGEKATPYDF 632
Query: 636 GAGEISPIKALNPGLVFESTNEDYLRFLCYYGYSNKIIRSVSK---QNFSCPKTSSEDLI 695
GAG+++ +PGL++E+ + DYL FL YYG+++ I+ +S Q F+CP+ S+ I
Sbjct: 633 GAGQVTIFGPSSPGLIYETNHMDYLNFLGYYGFTSDQIKKISNRIPQGFACPEQSNRGDI 692
Query: 696 SSVNYPSISIGKLDRKQAAKVIERTVTNV-----GAPNTTYIAKVHSSEGLLVKVIPSKI 755
S++NYPSISI + K++ +V RTVTNV G +T Y + + EGLLV+VIP ++
Sbjct: 693 SNINYPSISISNFNGKESRRV-SRTVTNVASRLIGDEDTVYTVSIDAPEGLLVRVIPRRL 752
Query: 756 VFSENVKKVTFKVSFYGKEA-RSGYNFGTITWRDSVHSVRTVFAV 782
F + K++++V F FG+ITW + +++VR+ F V
Sbjct: 753 HFRKIGDKLSYQVIFSSTTTILKDDAFGSITWSNGMYNVRSPFVV 770
BLAST of Lcy01g013470 vs. ExPASy Swiss-Prot
Match:
A9JQS7 (Subtilisin-like serine-protease S OS=Lotus japonicus OX=34305 GN=SbtS PE=2 SV=1)
HSP 1 Score: 516.5 bits (1329), Expect = 5.1e-145
Identity = 299/760 (39.34%), Postives = 449/760 (59.08%), Query Frame = 0
Query: 34 KHYVVYMGSGGSNYEEDDQTAAELDYLQLLSSVIPRKEKERGSRDVVHQYHNAFKGFSAM 93
KHY+VYMG E A ++L+SV + + +H Y +F+GFSAM
Sbjct: 26 KHYIVYMGDRSHPNSESVVRANH----EILASVTGSLNDAKAA--AIHHYSRSFQGFSAM 85
Query: 94 LTEEEASSLSGVDGIVSVFPDPTLQLHTTRSWDFLDSITGLRPPTPLPPPHSYLSSTDVI 153
+T E+A L+ + +VSVF +LHTT SWDFL T + P + S+++VI
Sbjct: 86 ITPEQAKKLADHNSVVSVFESKMNKLHTTHSWDFLGLDTVYK-----NNPSALDSASNVI 145
Query: 154 VGVVDTGIWPESQSFNDEGLGEIPSKWKGVCMEAPDFKKSNCNRKLVGARYYNAIEPNGN 213
VGV+D+G+WPES+SFND GLG +P K+KG C+ +F +NCN+K++GAR+Y+ G
Sbjct: 146 VGVIDSGVWPESESFNDYGLGPVPEKFKGECVTGDNFTLANCNKKIIGARFYS----KGL 205
Query: 214 DSRVGMPKG--------TPRDLLGHGTHTSSIAAGARVPNASYFDLARGTARGGGTVSTR 273
++ +G + +PRD GHGTHT+S AG+ V N S F +A+GTARGG S R
Sbjct: 206 EAEIGPLENIVDSIFFRSPRDSDGHGTHTASTIAGSIVSNVSLFGMAKGTARGGAP-SAR 265
Query: 274 IASYKVCAGVGCSGAAILKAIDDAVKDGVDIISISIGIGSPLFQSDYLNDPIAIGAFHAQ 333
++ YK C CS A + A+DDA+ DGVDI+S+S+G P Q Y + I++GAFHA
Sbjct: 266 LSIYKACWFGFCSDADVFAAMDDAIHDGVDILSLSLGPDPP--QPLYFENAISVGAFHAF 325
Query: 334 QMGVLVVCSGGNDGPDPNTVGNIAPWIFTVAASNIDRDFQSTVVLGNGKTFQGTAINLSN 393
Q G+LV S GN P T N+APWIFTVAAS +DR+F+S + LGN K +G ++N
Sbjct: 326 QKGILVSASAGN-SVFPRTACNVAPWIFTVAASTVDREFRSDIYLGNSKVLKGLSLNPIK 385
Query: 394 LTTSKTYPLVFGKDAAAKFTPISEARNCYPGSLDRSKVAGKIVICTSDDFSTSRTIKELV 453
+ S Y L++G AAA A C +LD + + GKIVICT + F+ +R K ++
Sbjct: 386 MEGS--YGLIYGSAAAAAGDAALNASFCKEHTLDPTLIKGKIVICTVEKFTDNRREKAII 445
Query: 454 VQDAKAMGLILINEASKTVPLDSNIFPFTQIGNSEGLQILEYINSTKNPTATILRTVEVR 513
++ +G+ILI+ ++ V + P T IG ++ Y+ + KNPTATI T+ +
Sbjct: 446 IKQGGGVGMILIDHNARDVGFQF-VIPSTMIGQDAVEELQAYMKTEKNPTATIFPTLTLV 505
Query: 514 RLKPAPMVAYFSSRGPSPLTENILKPDITAPGVSILAAVVPKSDAESGPIGKKPSDYAMR 573
KPAP A FSS GP+ +T +I+KPDIT PGV+ILAA P A + +K +Y +
Sbjct: 506 GTKPAPESAAFSSVGPNIITPDIIKPDITGPGVNILAAWSPV--ATEATVEQKSVNYNII 565
Query: 574 SGTSMACPHVAGAAAFVKSVRHNWSSSMIKSALMTTATIYDNQRKFM-RNSTNNPSNPHE 633
SGTSM+CPH++ +A +KS +WS + I SA+MT+AT+ DN + R+ + P +
Sbjct: 566 SGTSMSCPHISAISAIIKSHHPSWSPAAIMSAIMTSATVMDNTHSLIGRDPNGTQATPFD 625
Query: 634 MGAGEISPIKALNPGLVFESTNEDYLRFLCYYGYSNKIIRSVSKQNFSCPKTSSEDLISS 693
G+G ++P+ +LNPGLV++ +++D L FLC G S +++++ + C K+ + +
Sbjct: 626 YGSGHVNPVASLNPGLVYDFSSQDVLNFLCSNGASPAQLKNLTGELTQCQKSPTASY--N 685
Query: 694 VNYPSISIGKLDRKQAAKVIERTVTNVGAPNTTYIAKVHSSEGLLVKVIPSKIVFSENVK 753
NYPSI + L+ + + RTVT G T Y A V G++V+V P+K+ F + +
Sbjct: 686 FNYPSIGVSNLN---GSLSVYRTVTYYGQEPTEYFASVERPSGVIVRVTPAKLKFWKAGE 745
Query: 754 KVTFKVSFYG-KEARSGYNFGTITWRDSVHSVRTVFAVNV 784
K+TF++ F K + + FG +TW + VR+ +NV
Sbjct: 746 KITFRIDFTPFKNSNGNFVFGALTWNNGKQRVRSPIGLNV 756
BLAST of Lcy01g013470 vs. ExPASy Swiss-Prot
Match:
Q9FGU3 (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 SV=1)
HSP 1 Score: 498.0 bits (1281), Expect = 1.9e-139
Identity = 310/756 (41.01%), Postives = 426/756 (56.35%), Query Frame = 0
Query: 36 YVVYMGSGGSNYEEDDQTAAELDYLQLLSSVIPRKEKERGSRDVVHQYHNAFKGFSAMLT 95
Y+VY+GS S E + D++ +L + E +V Y +F GF+A LT
Sbjct: 35 YIVYLGSLPSREEYTPMS----DHMSILQEITGESLIE---NRLVRSYKKSFNGFAARLT 94
Query: 96 EEEASSLSGVDGIVSVFPDPTLQLHTTRSWDFLDSITGLRPPTPLPPPHSYLSSTDVIVG 155
E E L+G++ +VSVFP L+L TT SW+F+ G++ + +D I+G
Sbjct: 95 ESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGIK------TKRTRSIESDTIIG 154
Query: 156 VVDTGIWPESQSFNDEGLGEIPSKWKGVCMEAPDFKKSNCNRKLVGARYYNAIEPNGNDS 215
V+D+GI+PES SF+D+G G P KWKG C +F CN K++GAR Y A + N
Sbjct: 155 VIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNF---TCNNKVIGARDYTA-KSKANQ- 214
Query: 216 RVGMPKGTPRDLLGHGTHTSSIAAGARVPNASYFDLARGTARGGGTVSTRIASYKVCAGV 275
T RD GHGTHT+SIAAG V N++++ L GTAR GG + RIA YKVC
Sbjct: 215 -------TARDYSGHGTHTASIAAGNAVANSNFYGLGNGTAR-GGVPAARIAVYKVCDNE 274
Query: 276 GCSGAAILKAIDDAVKDGVDIISISIGIGS-PLFQSDYLNDPIAIGAFHAQQMGVLVVCS 335
GC G A++ A DDA+ DGVD+ISISI + + P F+ DPIAIGAFHA +GVL V +
Sbjct: 275 GCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFE----EDPIAIGAFHAMAVGVLTVNA 334
Query: 336 GGNDGPDPNTVGNIAPWIFTVAASNIDRDFQSTVVLGNGKTFQGTAINLSNLTTSKTYPL 395
GN+GP +TV + APW+F+VAAS +R F + VVLG+GK G ++N ++ YPL
Sbjct: 335 AGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDM-NGTNYPL 394
Query: 396 VFGKDAAAKFTPISEARNCYPGSLDRSKVAGKIVICTSDDFSTSRTIKELVVQDAKAMGL 455
V+GK AA + +AR C P LD V GKIV+C S T + Q A+G
Sbjct: 395 VYGKSAALSTCSVDKARLCEPKCLDGKLVKGKIVLCDS-------TKGLIEAQKLGAVGS 454
Query: 456 ILINEASKTVPLDSNIFPFTQIGNSEGLQILEYINSTKNPTATILRTVEVRRLKPAPMVA 515
I+ N + S FP + + N + ++ Y+NSTKNP AT+L++ E+ + AP+VA
Sbjct: 455 IVKNPEPDRAFIRS--FPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQR-APLVA 514
Query: 516 YFSSRGPSPLTENILKPDITAPGVSILAAVVPKSDAESGPIGKKPSDYAMRSGTSMACPH 575
FSSRGPS + +ILKPDITAPGV ILAA P S + Y++ SGTSMACPH
Sbjct: 515 SFSSRGPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPH 574
Query: 576 VAGAAAFVKSVRHNWSSSMIKSALMTTATIYDNQRKFMRNSTNNPSNPHEMGAGEISPIK 635
VAG AA+VK+ WS SMI+SA+MTTA + + S G+G + PI
Sbjct: 575 VAGVAAYVKTFHPQWSPSMIQSAIMTTAW------PMNASGSGFVSTEFAYGSGHVDPID 634
Query: 636 ALNPGLVFESTNEDYLRFLCYYGYSNKIIRSVSKQNFSCPKTSSEDLISSVNYPSISIGK 695
A+NPGLV+E T D++ FLC Y++ +R +S N +C K S+ L ++NYP++S
Sbjct: 635 AINPGLVYELTKADHINFLCGLNYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKV 694
Query: 696 LDRKQAAKVIERTVTNVGAPNTTYIAKVHSSEG--LLVKVIPSKIVFSENVKKVTFKVSF 755
K +RTVTNVG +TY AKV G L +KV P + +K +F V+
Sbjct: 695 SGTKPFNITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTV 737
Query: 756 YGKEARSGYNFGT-------ITWRDSVHSVRTVFAV 782
S + GT + W D H+VR+ V
Sbjct: 755 ------SSDSIGTKQPVSANLIWSDGTHNVRSPIIV 737
BLAST of Lcy01g013470 vs. ExPASy Swiss-Prot
Match:
Q8L7D2 (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)
HSP 1 Score: 496.5 bits (1277), Expect = 5.5e-139
Identity = 316/789 (40.05%), Postives = 448/789 (56.78%), Query Frame = 0
Query: 1 MASISLLLLHLLLLPLSLSANPSSIATLQNLPLKHYVVYMGSGGSNYEEDDQTAAELDYL 60
+A+ + L LL+L L SS++ + + + Y+VYMGS S + + D++
Sbjct: 4 LAASTCLYSWLLVLLL------SSVSAIIDEDTQVYIVYMGSLSSRADYIPTS----DHM 63
Query: 61 QLLSSVIPRKEKERGSRDVVHQYHNAFKGFSAMLTEEEASSLSGVDGIVSVFPDPTLQLH 120
+L V E +V Y +F GF+A LTE E + ++ ++G+VSVFP+ LQLH
Sbjct: 64 SILQQVTGESSIE---GRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLH 123
Query: 121 TTRSWDFLDSITGLRPPTPLPPPHSYLSSTDVIVGVVDTGIWPESQSFNDEGLGEIPSKW 180
TT SWDF+ G L +D I+GV+DTGIWPES+SF+D+G G P KW
Sbjct: 124 TTTSWDFMGVKEGKNTKRNL------AIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKW 183
Query: 181 KGVCMEAPDFKKSNCNRKLVGARYYNAIEPNGNDSRVGMPKGTPRDLLGHGTHTSSIAAG 240
KGVC +F CN KL+GAR Y + +GT RD GHGTHT+S AAG
Sbjct: 184 KGVCSGGKNF---TCNNKLIGARDYTS-------------EGT-RDTSGHGTHTASTAAG 243
Query: 241 ARVPNASYFDLARGTARGGGTVSTRIASYKVCAGVGCSGAAILKAIDDAVKDGVDIISIS 300
V + S+F + GT R GG ++RIA+YKVC GCS A+L + DDA+ DGVD+I+IS
Sbjct: 244 NAVKDTSFFGIGNGTVR-GGVPASRIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITIS 303
Query: 301 IGIGSPLFQSDYLNDPIAIGAFHAQQMGVLVVCSGGNDGPDPNTVGNIAPWIFTVAASNI 360
IG F S + +DPIAIGAFHA G+L V S GN GP P TV ++APWIFTVAAS
Sbjct: 304 IGF---QFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTVSHVAPWIFTVAASTT 363
Query: 361 DRDFQSTVVLGNGKTFQGTAINLSNLTTSKTYPLVFGKDAAAKFTPISEARNCYPGSLDR 420
+R F + VVLGNGKT G ++N ++ K YPLV+GK AA+ A C P L++
Sbjct: 364 NRGFITKVVLGNGKTLAGRSVNAFDM-KGKKYPLVYGKSAASSACDAKTAALCAPACLNK 423
Query: 421 SKVAGKIVICTSDDFSTSRTIKELVVQDAKAMGLI-LINEASKTVPLDSNIFPFTQIGNS 480
S+V GKI++C + AK++G I +I+++ + ++ P + +
Sbjct: 424 SRVKGKILVCGGPS----------GYKIAKSVGAIAIIDKSPRPDVAFTHHLPASGLKAK 483
Query: 481 EGLQILEYINSTKNPTATILRTVEVRRLKPAPMVAYFSSRGPSPLTENILKPDITAPGVS 540
+ ++ YI S +P A +L+T + + +P++A FSSRGP+ + +ILKPDITAPGV
Sbjct: 484 DFKSLVSYIESQDSPQAAVLKTETIFN-RTSPVIASFSSRGPNTIAVDILKPDITAPGVE 543
Query: 541 ILAAVVPKSDAESGPIGKKPSDYAMRSGTSMACPHVAGAAAFVKSVRHNWSSSMIKSALM 600
ILAA P + E + Y++ SGTSMACPHVAG AA+VK+ WS SMI+SA+M
Sbjct: 544 ILAAFSP--NGEPSEDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSAIM 603
Query: 601 TTATIYDNQRKFMRNSTNNPSNPHEMGAGEISPIKALNPGLVFESTNEDYLRFLCYYGYS 660
TTA S GAG + P+ ALNPGLV+E D++ FLC Y+
Sbjct: 604 TTAW------PVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYT 663
Query: 661 NKIIRSVSKQNFSCPKTSSEDLISSVNYPSISIGKLDRKQA--AKVIERTVTNVGAPNTT 720
+K ++ +S C K ++ L ++NYPS+S KL + + RT+TNVG PN+T
Sbjct: 664 SKTLKIISGDTVKCSK-KNKILPRNLNYPSMS-AKLSGTDSTFSVTFNRTLTNVGTPNST 723
Query: 721 YIAKVHSSEG--LLVKVIPSKIVFSENVKKVTFKVSFYGKEARSGY-NFGTITWRDSVHS 780
Y +KV + G L +KV PS + F +K +F V+ G + S + + W D H+
Sbjct: 724 YKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDGTHN 730
Query: 781 VRTVFAVNV 784
VR+ V +
Sbjct: 784 VRSPIVVYI 730
BLAST of Lcy01g013470 vs. ExPASy TrEMBL
Match:
A0A6J1HAX7 (CO(2)-response secreted protease-like OS=Cucurbita moschata OX=3662 GN=LOC111462415 PE=3 SV=1)
HSP 1 Score: 1408.7 bits (3645), Expect = 0.0e+00
Identity = 709/781 (90.78%), Postives = 743/781 (95.13%), Query Frame = 0
Query: 6 LLLLHLLLLPLSLSANPSSIATLQNLPLKHYVVYMGSGGSNYEEDDQTAAELDYLQLLSS 65
LLLLH LLP SLSA PS+IA LQNLPLKHYVVYMG G S+ EE ++TA ELD+LQLLSS
Sbjct: 23 LLLLH--LLPPSLSATPSAIAALQNLPLKHYVVYMGGGSSSNEEHEETAGELDFLQLLSS 82
Query: 66 VIP-RKEKERGSRDVVHQYHNAFKGFSAMLTEEEASSLSGVDGIVSVFPDPTLQLHTTRS 125
V P R+EKERGSRD++HQYH+AFKGFSAMLTEEEASSLSGVDGIVSVFPDPTLQLHTTRS
Sbjct: 83 VNPRRREKERGSRDLIHQYHHAFKGFSAMLTEEEASSLSGVDGIVSVFPDPTLQLHTTRS 142
Query: 126 WDFLDSITGLRPPTPLPPPHSYLSSTDVIVGVVDTGIWPESQSFNDEGLGEIPSKWKGVC 185
WDFLDSI+GLRPPTPLPPPHSY SS+DV+VG++DTGIWPESQSFNDEG+GEIPSKWKGVC
Sbjct: 143 WDFLDSISGLRPPTPLPPPHSYASSSDVVVGIIDTGIWPESQSFNDEGIGEIPSKWKGVC 202
Query: 186 MEAPDFKKSNCNRKLVGARYYNAIEPNGNDSRVGMPKGTPRDLLGHGTHTSSIAAGARVP 245
MEAPDFKKSNCNRKL+GARYYN +EPNGNDSR +PKGTPRD LGHGTHTSSIAAGARVP
Sbjct: 203 MEAPDFKKSNCNRKLIGARYYNVVEPNGNDSRGSVPKGTPRDSLGHGTHTSSIAAGARVP 262
Query: 246 NASYFDLARGTARGGGTVSTRIASYKVCAGVGCSGAAILKAIDDAVKDGVDIISISIGIG 305
NASYF LARGTARGGG STRIASYKVCAGVGCSGAAILKAIDDAVKDGVDIISISIGIG
Sbjct: 263 NASYFGLARGTARGGGIPSTRIASYKVCAGVGCSGAAILKAIDDAVKDGVDIISISIGIG 322
Query: 306 SPLFQSDYLNDPIAIGAFHAQQMGVLVVCSGGNDGPDPNTVGNIAPWIFTVAASNIDRDF 365
SPLFQSDYLNDPIAIGAFHAQQMGVLVVCS GNDGPDPNTVGN+APWIFTVAASNIDRDF
Sbjct: 323 SPLFQSDYLNDPIAIGAFHAQQMGVLVVCSAGNDGPDPNTVGNVAPWIFTVAASNIDRDF 382
Query: 366 QSTVVLGNGKTFQGTAINLSNLTTSKTYPLVFGKDAAAKFTPISEARNCYPGSLDRSKVA 425
QSTVVLGNGKTFQGTAINLSNLT+SKTYPLVFGKDAAAKFTPISEARNCYPGSLDRSKVA
Sbjct: 383 QSTVVLGNGKTFQGTAINLSNLTSSKTYPLVFGKDAAAKFTPISEARNCYPGSLDRSKVA 442
Query: 426 GKIVICTSDDFSTSRTIKELVVQDAKAMGLILINEASKTVPLDSNIFPFTQIGNSEGLQI 485
GKIV+C SDDFSTSRTIKELVVQDAKA+GLILINEASKTVP+DSNIFPFTQIGNSEGLQI
Sbjct: 443 GKIVVCASDDFSTSRTIKELVVQDAKAVGLILINEASKTVPMDSNIFPFTQIGNSEGLQI 502
Query: 486 LEYINSTKNPTATILRTVEVRRLKPAPMVAYFSSRGPSPLTENILKPDITAPGVSILAAV 545
LEYINSTKNPTATILRTVEVRRLKPAP+VAYFSSRGPSPLTENILKPDI APGVSILAA+
Sbjct: 503 LEYINSTKNPTATILRTVEVRRLKPAPVVAYFSSRGPSPLTENILKPDIMAPGVSILAAI 562
Query: 546 VPKSDAESGPIGKKPSDYAMRSGTSMACPHVAGAAAFVKSVRHNWSSSMIKSALMTTATI 605
PKSDAESGPIGKKPS+YAMRSGTSM+CPHVAGAAAFVKSV HNWSSSMIKSALMTTAT+
Sbjct: 563 APKSDAESGPIGKKPSNYAMRSGTSMSCPHVAGAAAFVKSVYHNWSSSMIKSALMTTATL 622
Query: 606 YDNQRKFMRNSTNNPSNPHEMGAGEISPIKALNPGLVFESTNEDYLRFLCYYGYSNKIIR 665
YDNQRKFMRNSTNNPSNPHEMGAGEISPIKALNPGLV+ESTNEDYLRFLCYYGYSNKIIR
Sbjct: 623 YDNQRKFMRNSTNNPSNPHEMGAGEISPIKALNPGLVYESTNEDYLRFLCYYGYSNKIIR 682
Query: 666 SVSKQNFSCPKTSSEDLISSVNYPSISIGKLDRKQAA--KVIERTVTNVGAPNTTYIAKV 725
SVSKQNFSCPKTS E LISSVNYPSISI KLDRK A V+ERTVTNVGAP+ TYIAKV
Sbjct: 683 SVSKQNFSCPKTSKEYLISSVNYPSISISKLDRKVAGGKVVVERTVTNVGAPDATYIAKV 742
Query: 726 HSSEGLLVKVIPSKIVFSENVKKVTFKVSFYGKEARSGYNFGTITWRDSVHSVRTVFAVN 784
HSSEGL+VKV PSKIVF+E VKKVTFKVSFYGKE RSGYNFGTITWRD+ HSVRTVFAVN
Sbjct: 743 HSSEGLIVKVTPSKIVFTEKVKKVTFKVSFYGKEVRSGYNFGTITWRDTAHSVRTVFAVN 801
BLAST of Lcy01g013470 vs. ExPASy TrEMBL
Match:
A0A6J1K3Y1 (CO(2)-response secreted protease-like OS=Cucurbita maxima OX=3661 GN=LOC111492101 PE=3 SV=1)
HSP 1 Score: 1403.3 bits (3631), Expect = 0.0e+00
Identity = 704/781 (90.14%), Postives = 742/781 (95.01%), Query Frame = 0
Query: 6 LLLLHLLLLPLSLSANPSSIATLQNLPLKHYVVYMGSGGSNYEEDDQTAAELDYLQLLSS 65
LLLL L LLP SLSA PS+IA LQNLPLKHYVVYMG G S+ EE +Q A ELD+LQLLSS
Sbjct: 23 LLLLLLHLLPPSLSATPSAIAALQNLPLKHYVVYMGGGSSSNEEHEQRAGELDFLQLLSS 82
Query: 66 VIP-RKEKERGSRDVVHQYHNAFKGFSAMLTEEEASSLSGVDGIVSVFPDPTLQLHTTRS 125
V P R+EKERGSRD++HQYH+AFKGFSAMLTEEEASSLSG+DGIVSVFPDPTLQLHTTRS
Sbjct: 83 VNPRRREKERGSRDLIHQYHHAFKGFSAMLTEEEASSLSGIDGIVSVFPDPTLQLHTTRS 142
Query: 126 WDFLDSITGLRPPTPLPPPHSYLSSTDVIVGVVDTGIWPESQSFNDEGLGEIPSKWKGVC 185
WDFLDSI+GLRPPTPLPPPHSY SS+DV+VG++DTGIWPESQSFNDEG+GEIPSKWKGVC
Sbjct: 143 WDFLDSISGLRPPTPLPPPHSYASSSDVVVGIIDTGIWPESQSFNDEGIGEIPSKWKGVC 202
Query: 186 MEAPDFKKSNCNRKLVGARYYNAIEPNGNDSRVGMPKGTPRDLLGHGTHTSSIAAGARVP 245
MEAPDFKKSNCNRKL+GARYYN +EPNGNDS +PKGTPRD LGHGTHTSSIAAG+RVP
Sbjct: 203 MEAPDFKKSNCNRKLIGARYYNVVEPNGNDSHGSLPKGTPRDSLGHGTHTSSIAAGSRVP 262
Query: 246 NASYFDLARGTARGGGTVSTRIASYKVCAGVGCSGAAILKAIDDAVKDGVDIISISIGIG 305
NASYF LARGTARGGG STRIASYKVCAGVGCSGAAILKAIDDAVKDGVDIISISIGIG
Sbjct: 263 NASYFGLARGTARGGGIPSTRIASYKVCAGVGCSGAAILKAIDDAVKDGVDIISISIGIG 322
Query: 306 SPLFQSDYLNDPIAIGAFHAQQMGVLVVCSGGNDGPDPNTVGNIAPWIFTVAASNIDRDF 365
SPLFQSDYLNDPIAIGAFHAQQMGVLVVCS GNDGPDPNTVGN+APWIFTVAASNIDRDF
Sbjct: 323 SPLFQSDYLNDPIAIGAFHAQQMGVLVVCSAGNDGPDPNTVGNVAPWIFTVAASNIDRDF 382
Query: 366 QSTVVLGNGKTFQGTAINLSNLTTSKTYPLVFGKDAAAKFTPISEARNCYPGSLDRSKVA 425
QSTVVLGNGKTFQGTAINLSNLT+SKTYPLVFGKDAAAKFTP+SEARNCYPGSLDRSKVA
Sbjct: 383 QSTVVLGNGKTFQGTAINLSNLTSSKTYPLVFGKDAAAKFTPMSEARNCYPGSLDRSKVA 442
Query: 426 GKIVICTSDDFSTSRTIKELVVQDAKAMGLILINEASKTVPLDSNIFPFTQIGNSEGLQI 485
GKIV+C SDDFSTSRTIKELVVQDAKA+GLILINEASKTVP+DSNIFPFTQIGNSEGLQI
Sbjct: 443 GKIVVCASDDFSTSRTIKELVVQDAKAVGLILINEASKTVPMDSNIFPFTQIGNSEGLQI 502
Query: 486 LEYINSTKNPTATILRTVEVRRLKPAPMVAYFSSRGPSPLTENILKPDITAPGVSILAAV 545
LEYINSTKNPTATILRTVEVRRLKPAP+VAYFSSRGPSPLTENILKPDI APGVSILAA+
Sbjct: 503 LEYINSTKNPTATILRTVEVRRLKPAPVVAYFSSRGPSPLTENILKPDIMAPGVSILAAI 562
Query: 546 VPKSDAESGPIGKKPSDYAMRSGTSMACPHVAGAAAFVKSVRHNWSSSMIKSALMTTATI 605
PKSDAESGPIGKKPS+YAMRSGTSM+CPHVAGAAAFVKSV HNWSSSMIKSALMTTAT+
Sbjct: 563 APKSDAESGPIGKKPSNYAMRSGTSMSCPHVAGAAAFVKSVYHNWSSSMIKSALMTTATL 622
Query: 606 YDNQRKFMRNSTNNPSNPHEMGAGEISPIKALNPGLVFESTNEDYLRFLCYYGYSNKIIR 665
YDNQRKFMRNSTNNPSNPHEMGAGEISPIKALNPGLV+ESTNEDYLRFLCYYGYSNKIIR
Sbjct: 623 YDNQRKFMRNSTNNPSNPHEMGAGEISPIKALNPGLVYESTNEDYLRFLCYYGYSNKIIR 682
Query: 666 SVSKQNFSCPKTSSEDLISSVNYPSISIGKLDRKQAA--KVIERTVTNVGAPNTTYIAKV 725
SVSKQNFSCPKTS E LISS+NYPSISI KLDRK AA V+ERTVTNVGAP+ TYIAKV
Sbjct: 683 SVSKQNFSCPKTSKEYLISSINYPSISISKLDRKVAAGKVVVERTVTNVGAPDATYIAKV 742
Query: 726 HSSEGLLVKVIPSKIVFSENVKKVTFKVSFYGKEARSGYNFGTITWRDSVHSVRTVFAVN 784
HSSEGL+VKV PSKI F+E VKKVTFKVSFYGKEAR+GYNFGTITWRD+ HSVRTVFAVN
Sbjct: 743 HSSEGLIVKVTPSKITFTEKVKKVTFKVSFYGKEARNGYNFGTITWRDTAHSVRTVFAVN 802
BLAST of Lcy01g013470 vs. ExPASy TrEMBL
Match:
A0A5A7VC87 (CO(2)-response secreted protease-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G00970 PE=3 SV=1)
HSP 1 Score: 1369.4 bits (3543), Expect = 0.0e+00
Identity = 697/793 (87.89%), Postives = 739/793 (93.19%), Query Frame = 0
Query: 1 MASISLLLLH-----LLLLPLSLSANPSSIATLQNLPLKHYVVYMGSGGSNYEEDDQTA- 60
MAS S LLL LLLL L LSAN SSIATLQNLPLKHYVVYMG+G ED++TA
Sbjct: 1 MASSSHLLLFFHLLLLLLLLLPLSANSSSIATLQNLPLKHYVVYMGNG-----EDEETAG 60
Query: 61 AELDYLQLLSSVIP---RKEKERGSRDVV-HQYHNAFKGFSAMLTEEEASSLSGVDGIVS 120
ELDYLQLLSSVIP KEKE GSRDVV HQYH+AFKGFSAMLTEEEASSLSG+DGIVS
Sbjct: 61 GELDYLQLLSSVIPSRKEKEKENGSRDVVIHQYHHAFKGFSAMLTEEEASSLSGIDGIVS 120
Query: 121 VFPDPTLQLHTTRSWDFLDSITGLRPPTPLPPPHSYLSSTDVIVGVVDTGIWPESQSFND 180
VFPDPTLQLHTTRSWDFLDSI+GLRPPTPLPPPH Y SS+DVIVGV+DTGIWPESQSFND
Sbjct: 121 VFPDPTLQLHTTRSWDFLDSISGLRPPTPLPPPHFYPSSSDVIVGVIDTGIWPESQSFND 180
Query: 181 EGLGEIPSKWKGVCMEAPDFKKSNCNRKLVGARYYNAIEPNGNDSRVGMPKGTPRDLLGH 240
EG+GEIPSKWKGVCMEAPDFKKSNCNRKL+GARYYN +E NGNDS VG PKGTPRD LGH
Sbjct: 181 EGVGEIPSKWKGVCMEAPDFKKSNCNRKLIGARYYNVVELNGNDSHVGPPKGTPRDSLGH 240
Query: 241 GTHTSSIAAGARVPNASYFDLARGTARGGGTVSTRIASYKVCAGVGCSGAAILKAIDDAV 300
G+HTSSIAAGARVPNASYF LARGTARGGGT STRIASYKVCAGVGCSGAAILKAIDDA+
Sbjct: 241 GSHTSSIAAGARVPNASYFGLARGTARGGGTPSTRIASYKVCAGVGCSGAAILKAIDDAI 300
Query: 301 KDGVDIISISIGIGSPLFQSDYLNDPIAIGAFHAQQMGVLVVCSGGNDGPDPNTVGNIAP 360
KDGVDIISISIGIGSPLFQSDYLNDPIAIGA HAQ GVLVVCS GNDGPDPNTVGN+AP
Sbjct: 301 KDGVDIISISIGIGSPLFQSDYLNDPIAIGALHAQLRGVLVVCSAGNDGPDPNTVGNVAP 360
Query: 361 WIFTVAASNIDRDFQSTVVLGNGKTFQGTAINLSNLTTSKTYPLVFGKDAAAKFTPISEA 420
WIFTVAASNIDRDFQSTVVLGNGKTF GT INLSNLT+SKTYPLVFGKDAAAKFTP SEA
Sbjct: 361 WIFTVAASNIDRDFQSTVVLGNGKTFHGTGINLSNLTSSKTYPLVFGKDAAAKFTPTSEA 420
Query: 421 RNCYPGSLDRSKVAGKIVICTSDDFSTSRTIKELVVQDAKAMGLILINEASKTVPLDSNI 480
RNCYPGSLDRSKVAGKIV+C SDDFSTSRTIKELVVQDAKA+GLILINEASK+VP+DSNI
Sbjct: 421 RNCYPGSLDRSKVAGKIVVCASDDFSTSRTIKELVVQDAKAVGLILINEASKSVPMDSNI 480
Query: 481 FPFTQIGNSEGLQILEYINSTKNPTATILRTVEVRRLKPAPMVAYFSSRGPSPLTENILK 540
FPFTQIGNSEGLQILEYINSTKNPTATIL+TVEVRRLKPAP VAYFSSRGPSPLTENILK
Sbjct: 481 FPFTQIGNSEGLQILEYINSTKNPTATILKTVEVRRLKPAPTVAYFSSRGPSPLTENILK 540
Query: 541 PDITAPGVSILAAVVPKSDAESGPIGKKPSDYAMRSGTSMACPHVAGAAAFVKSVRHNWS 600
PDITAPGVSILAA++PKSD ++GPIGKKPS+YAM+SGTSMACPHVAGAAAF+KSV H+WS
Sbjct: 541 PDITAPGVSILAAMIPKSDGDTGPIGKKPSNYAMKSGTSMACPHVAGAAAFIKSVYHDWS 600
Query: 601 SSMIKSALMTTATIYDNQRKFMRNSTNNPSNPHEMGAGEISPIKALNPGLVFESTNEDYL 660
SSMIKSALMTTAT YDNQRK+MRN+TNNPSNPHEMGAGEISPIKALNPGLVFE+TNEDYL
Sbjct: 601 SSMIKSALMTTATQYDNQRKYMRNTTNNPSNPHEMGAGEISPIKALNPGLVFETTNEDYL 660
Query: 661 RFLCYYGYSNKIIRSVSKQNFSCPKTSSEDLISSVNYPSISIGKLDRKQAAKVIERTVTN 720
FLCYYGYSNK++RS+ KQNF+CPKTS EDLIS+VNYPSISIGKLDRKQAAKV+ERTVTN
Sbjct: 661 LFLCYYGYSNKVLRSMLKQNFTCPKTSKEDLISNVNYPSISIGKLDRKQAAKVVERTVTN 720
Query: 721 VGAPNTTYIAKVHSSEGLLVKVIPSKIVFSENVKKVTFKVSFYGKEARSGYNFGTITWRD 780
VGAP+ TYIAKVHSSEGL+VKV P KIVFSE VKKVTFKVSFYGKEAR+GYNFG+ITWRD
Sbjct: 721 VGAPDATYIAKVHSSEGLIVKVNPRKIVFSEKVKKVTFKVSFYGKEARNGYNFGSITWRD 780
Query: 781 SVHSVRTVFAVNV 784
+ HSVRT FAVNV
Sbjct: 781 TAHSVRTFFAVNV 788
BLAST of Lcy01g013470 vs. ExPASy TrEMBL
Match:
A0A6J1BRZ1 (CO(2)-response secreted protease-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111005013 PE=3 SV=1)
HSP 1 Score: 1367.1 bits (3537), Expect = 0.0e+00
Identity = 696/785 (88.66%), Postives = 736/785 (93.76%), Query Frame = 0
Query: 1 MASISLLLLHLLLLPLSLSANPSSIATLQNLPLKHYVVYMGSGGSNY-EEDDQTAAELDY 60
MAS+ LLL LLLL LSA SSIATLQN KHYVVYMGSGGSN EDDQTAAELDY
Sbjct: 1 MASLPHLLLLLLLLLFFLSAVSSSIATLQNHLPKHYVVYMGSGGSNNGGEDDQTAAELDY 60
Query: 61 LQLLSSVIPRKEKERGSRDVVHQYHNAFKGFSAMLTEEEASSLSGVDGIVSVFPDPTLQL 120
LQLLS++IPRKEKE SR+VVHQYH+AF+GFSAMLTEEEASSLSG+DGIVSVFPDPTLQL
Sbjct: 61 LQLLSTLIPRKEKEERSREVVHQYHHAFRGFSAMLTEEEASSLSGIDGIVSVFPDPTLQL 120
Query: 121 HTTRSWDFLDSITGLRPPTPLPPPHSYLSSTDVIVGVVDTGIWPESQSFNDEGLGEIPSK 180
HTTRSWDFLDSI GLR PPPHSY SS+DVIVGV+DTGIWPES+SF+DEGLGEIPSK
Sbjct: 121 HTTRSWDFLDSIAGLR-----PPPHSYPSSSDVIVGVIDTGIWPESRSFSDEGLGEIPSK 180
Query: 181 WKGVCMEAPDFKKSNCNRKLVGARYYNAIEPNGNDSRVGMPKGTPRDLLGHGTHTSSIAA 240
WKG+CMEA DFKKSNCNRKL+GARYYNAIEPNGNDSRVG+PKGTPRD LGHGTHTSSIAA
Sbjct: 181 WKGICMEASDFKKSNCNRKLIGARYYNAIEPNGNDSRVGVPKGTPRDSLGHGTHTSSIAA 240
Query: 241 GARVPNASYFDLARGTARGGGTVSTRIASYKVCAGVGCSGAAILKAIDDAVKDGVDIISI 300
GARV + SYF LARGTARGGG STRIASYKVC+GVGCSGAAILKAIDDAVKDGVDIISI
Sbjct: 241 GARVLDTSYFGLARGTARGGGAPSTRIASYKVCSGVGCSGAAILKAIDDAVKDGVDIISI 300
Query: 301 SIGIGSPLFQSDYLNDPIAIGAFHAQQMGVLVVCSGGNDGPDPNTVGNIAPWIFTVAASN 360
SIGIGSPLFQSDYLNDPIAIGAFHA QMGVLVVCS GNDGPDPNTVGN+APWI TVAASN
Sbjct: 301 SIGIGSPLFQSDYLNDPIAIGAFHAHQMGVLVVCSAGNDGPDPNTVGNVAPWILTVAASN 360
Query: 361 IDRDFQSTVVLGNGKTFQGTAINLSNLTTSKTYPLVFGKDAAAKFTPISEARNCYPGSLD 420
IDRDFQSTVVLGNGKTFQGTAINLSNLT+SKTYPLVFGKDAAAKFTPISEARNCYPGSLD
Sbjct: 361 IDRDFQSTVVLGNGKTFQGTAINLSNLTSSKTYPLVFGKDAAAKFTPISEARNCYPGSLD 420
Query: 421 RSKVAGKIVICTSDDFSTSRTIKELVVQDAKAMGLILINEASKTVPLDSNIFPFTQIGNS 480
R+KVAGKIV+C S+D +TSRTIKELVVQDAKA+GLILINEASKTVP+DSNIFP TQ+GNS
Sbjct: 421 RAKVAGKIVVCASEDVTTSRTIKELVVQDAKAIGLILINEASKTVPVDSNIFPLTQVGNS 480
Query: 481 EGLQILEYINSTKNPTATILRTVEVRRLKPAPMVAYFSSRGPSPLTENILKPDITAPGVS 540
EGLQILEYINSTKNPTATILRTVEV R KPAP+VAYFSSRGPS LTENILKPDITAPGVS
Sbjct: 481 EGLQILEYINSTKNPTATILRTVEVPRFKPAPLVAYFSSRGPSSLTENILKPDITAPGVS 540
Query: 541 ILAAVVPKSDAESGPIGKKPSDYAMRSGTSMACPHVAGAAAFVKSVRHNWSSSMIKSALM 600
ILAA+VPKS+A+SG IGKK SDYAMRSGTSMACPHVAGAAAF+K + H+WSSSMIKSALM
Sbjct: 541 ILAAIVPKSEADSGLIGKKSSDYAMRSGTSMACPHVAGAAAFIKLIHHDWSSSMIKSALM 600
Query: 601 TTATIYDNQRKFMRNSTNNPSNPHEMGAGEISPIKALNPGLVFESTNEDYLRFLCYYGYS 660
TTAT+YDNQRKFMRNSTNNPSNPHEMGAGEISPIKALNPGLVFEST EDYLRFLCY+GYS
Sbjct: 601 TTATLYDNQRKFMRNSTNNPSNPHEMGAGEISPIKALNPGLVFESTTEDYLRFLCYFGYS 660
Query: 661 NKIIRSVSKQNFSCPKTSSEDLISSVNYPSISIGKLDRKQAAKVIERTVTNVGAPNTTYI 720
NKIIRS+SKQNF+CPKTS+EDLISSVNYPSISI KLDR+QA KVIERTVTNVGA NTTYI
Sbjct: 661 NKIIRSMSKQNFTCPKTSNEDLISSVNYPSISISKLDRRQAPKVIERTVTNVGAANTTYI 720
Query: 721 AKVHSSEGLLVKVIPSKIVFSENVKKVTFKVSFYGKEARSGYNFGTITWRDSVHSVRTVF 780
AKVHSSEGL+VKVIPSKIVFSENVKKVTFKV F+GKEARSGYNFGTITWRD VHSVRTVF
Sbjct: 721 AKVHSSEGLIVKVIPSKIVFSENVKKVTFKVLFFGKEARSGYNFGTITWRDDVHSVRTVF 780
Query: 781 AVNVE 785
AVNVE
Sbjct: 781 AVNVE 780
BLAST of Lcy01g013470 vs. ExPASy TrEMBL
Match:
A0A6J1BV63 (CO(2)-response secreted protease-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005013 PE=3 SV=1)
HSP 1 Score: 1361.7 bits (3523), Expect = 0.0e+00
Identity = 696/788 (88.32%), Postives = 736/788 (93.40%), Query Frame = 0
Query: 1 MASISLLLLHLLLLPLSLSANPSSIATLQNLPLKHYVVYMGSGGSNY-EEDDQTAAELDY 60
MAS+ LLL LLLL LSA SSIATLQN KHYVVYMGSGGSN EDDQTAAELDY
Sbjct: 1 MASLPHLLLLLLLLLFFLSAVSSSIATLQNHLPKHYVVYMGSGGSNNGGEDDQTAAELDY 60
Query: 61 LQLLSSVIP---RKEKERGSRDVVHQYHNAFKGFSAMLTEEEASSLSGVDGIVSVFPDPT 120
LQLLS++IP RKEKE SR+VVHQYH+AF+GFSAMLTEEEASSLSG+DGIVSVFPDPT
Sbjct: 61 LQLLSTLIPRKNRKEKEERSREVVHQYHHAFRGFSAMLTEEEASSLSGIDGIVSVFPDPT 120
Query: 121 LQLHTTRSWDFLDSITGLRPPTPLPPPHSYLSSTDVIVGVVDTGIWPESQSFNDEGLGEI 180
LQLHTTRSWDFLDSI GLR PPPHSY SS+DVIVGV+DTGIWPES+SF+DEGLGEI
Sbjct: 121 LQLHTTRSWDFLDSIAGLR-----PPPHSYPSSSDVIVGVIDTGIWPESRSFSDEGLGEI 180
Query: 181 PSKWKGVCMEAPDFKKSNCNRKLVGARYYNAIEPNGNDSRVGMPKGTPRDLLGHGTHTSS 240
PSKWKG+CMEA DFKKSNCNRKL+GARYYNAIEPNGNDSRVG+PKGTPRD LGHGTHTSS
Sbjct: 181 PSKWKGICMEASDFKKSNCNRKLIGARYYNAIEPNGNDSRVGVPKGTPRDSLGHGTHTSS 240
Query: 241 IAAGARVPNASYFDLARGTARGGGTVSTRIASYKVCAGVGCSGAAILKAIDDAVKDGVDI 300
IAAGARV + SYF LARGTARGGG STRIASYKVC+GVGCSGAAILKAIDDAVKDGVDI
Sbjct: 241 IAAGARVLDTSYFGLARGTARGGGAPSTRIASYKVCSGVGCSGAAILKAIDDAVKDGVDI 300
Query: 301 ISISIGIGSPLFQSDYLNDPIAIGAFHAQQMGVLVVCSGGNDGPDPNTVGNIAPWIFTVA 360
ISISIGIGSPLFQSDYLNDPIAIGAFHA QMGVLVVCS GNDGPDPNTVGN+APWI TVA
Sbjct: 301 ISISIGIGSPLFQSDYLNDPIAIGAFHAHQMGVLVVCSAGNDGPDPNTVGNVAPWILTVA 360
Query: 361 ASNIDRDFQSTVVLGNGKTFQGTAINLSNLTTSKTYPLVFGKDAAAKFTPISEARNCYPG 420
ASNIDRDFQSTVVLGNGKTFQGTAINLSNLT+SKTYPLVFGKDAAAKFTPISEARNCYPG
Sbjct: 361 ASNIDRDFQSTVVLGNGKTFQGTAINLSNLTSSKTYPLVFGKDAAAKFTPISEARNCYPG 420
Query: 421 SLDRSKVAGKIVICTSDDFSTSRTIKELVVQDAKAMGLILINEASKTVPLDSNIFPFTQI 480
SLDR+KVAGKIV+C S+D +TSRTIKELVVQDAKA+GLILINEASKTVP+DSNIFP TQ+
Sbjct: 421 SLDRAKVAGKIVVCASEDVTTSRTIKELVVQDAKAIGLILINEASKTVPVDSNIFPLTQV 480
Query: 481 GNSEGLQILEYINSTKNPTATILRTVEVRRLKPAPMVAYFSSRGPSPLTENILKPDITAP 540
GNSEGLQILEYINSTKNPTATILRTVEV R KPAP+VAYFSSRGPS LTENILKPDITAP
Sbjct: 481 GNSEGLQILEYINSTKNPTATILRTVEVPRFKPAPLVAYFSSRGPSSLTENILKPDITAP 540
Query: 541 GVSILAAVVPKSDAESGPIGKKPSDYAMRSGTSMACPHVAGAAAFVKSVRHNWSSSMIKS 600
GVSILAA+VPKS+A+SG IGKK SDYAMRSGTSMACPHVAGAAAF+K + H+WSSSMIKS
Sbjct: 541 GVSILAAIVPKSEADSGLIGKKSSDYAMRSGTSMACPHVAGAAAFIKLIHHDWSSSMIKS 600
Query: 601 ALMTTATIYDNQRKFMRNSTNNPSNPHEMGAGEISPIKALNPGLVFESTNEDYLRFLCYY 660
ALMTTAT+YDNQRKFMRNSTNNPSNPHEMGAGEISPIKALNPGLVFEST EDYLRFLCY+
Sbjct: 601 ALMTTATLYDNQRKFMRNSTNNPSNPHEMGAGEISPIKALNPGLVFESTTEDYLRFLCYF 660
Query: 661 GYSNKIIRSVSKQNFSCPKTSSEDLISSVNYPSISIGKLDRKQAAKVIERTVTNVGAPNT 720
GYSNKIIRS+SKQNF+CPKTS+EDLISSVNYPSISI KLDR+QA KVIERTVTNVGA NT
Sbjct: 661 GYSNKIIRSMSKQNFTCPKTSNEDLISSVNYPSISISKLDRRQAPKVIERTVTNVGAANT 720
Query: 721 TYIAKVHSSEGLLVKVIPSKIVFSENVKKVTFKVSFYGKEARSGYNFGTITWRDSVHSVR 780
TYIAKVHSSEGL+VKVIPSKIVFSENVKKVTFKV F+GKEARSGYNFGTITWRD VHSVR
Sbjct: 721 TYIAKVHSSEGLIVKVIPSKIVFSENVKKVTFKVLFFGKEARSGYNFGTITWRDDVHSVR 780
Query: 781 TVFAVNVE 785
TVFAVNVE
Sbjct: 781 TVFAVNVE 783
BLAST of Lcy01g013470 vs. NCBI nr
Match:
XP_022961732.1 (CO(2)-response secreted protease-like [Cucurbita moschata])
HSP 1 Score: 1408.7 bits (3645), Expect = 0.0e+00
Identity = 709/781 (90.78%), Postives = 743/781 (95.13%), Query Frame = 0
Query: 6 LLLLHLLLLPLSLSANPSSIATLQNLPLKHYVVYMGSGGSNYEEDDQTAAELDYLQLLSS 65
LLLLH LLP SLSA PS+IA LQNLPLKHYVVYMG G S+ EE ++TA ELD+LQLLSS
Sbjct: 23 LLLLH--LLPPSLSATPSAIAALQNLPLKHYVVYMGGGSSSNEEHEETAGELDFLQLLSS 82
Query: 66 VIP-RKEKERGSRDVVHQYHNAFKGFSAMLTEEEASSLSGVDGIVSVFPDPTLQLHTTRS 125
V P R+EKERGSRD++HQYH+AFKGFSAMLTEEEASSLSGVDGIVSVFPDPTLQLHTTRS
Sbjct: 83 VNPRRREKERGSRDLIHQYHHAFKGFSAMLTEEEASSLSGVDGIVSVFPDPTLQLHTTRS 142
Query: 126 WDFLDSITGLRPPTPLPPPHSYLSSTDVIVGVVDTGIWPESQSFNDEGLGEIPSKWKGVC 185
WDFLDSI+GLRPPTPLPPPHSY SS+DV+VG++DTGIWPESQSFNDEG+GEIPSKWKGVC
Sbjct: 143 WDFLDSISGLRPPTPLPPPHSYASSSDVVVGIIDTGIWPESQSFNDEGIGEIPSKWKGVC 202
Query: 186 MEAPDFKKSNCNRKLVGARYYNAIEPNGNDSRVGMPKGTPRDLLGHGTHTSSIAAGARVP 245
MEAPDFKKSNCNRKL+GARYYN +EPNGNDSR +PKGTPRD LGHGTHTSSIAAGARVP
Sbjct: 203 MEAPDFKKSNCNRKLIGARYYNVVEPNGNDSRGSVPKGTPRDSLGHGTHTSSIAAGARVP 262
Query: 246 NASYFDLARGTARGGGTVSTRIASYKVCAGVGCSGAAILKAIDDAVKDGVDIISISIGIG 305
NASYF LARGTARGGG STRIASYKVCAGVGCSGAAILKAIDDAVKDGVDIISISIGIG
Sbjct: 263 NASYFGLARGTARGGGIPSTRIASYKVCAGVGCSGAAILKAIDDAVKDGVDIISISIGIG 322
Query: 306 SPLFQSDYLNDPIAIGAFHAQQMGVLVVCSGGNDGPDPNTVGNIAPWIFTVAASNIDRDF 365
SPLFQSDYLNDPIAIGAFHAQQMGVLVVCS GNDGPDPNTVGN+APWIFTVAASNIDRDF
Sbjct: 323 SPLFQSDYLNDPIAIGAFHAQQMGVLVVCSAGNDGPDPNTVGNVAPWIFTVAASNIDRDF 382
Query: 366 QSTVVLGNGKTFQGTAINLSNLTTSKTYPLVFGKDAAAKFTPISEARNCYPGSLDRSKVA 425
QSTVVLGNGKTFQGTAINLSNLT+SKTYPLVFGKDAAAKFTPISEARNCYPGSLDRSKVA
Sbjct: 383 QSTVVLGNGKTFQGTAINLSNLTSSKTYPLVFGKDAAAKFTPISEARNCYPGSLDRSKVA 442
Query: 426 GKIVICTSDDFSTSRTIKELVVQDAKAMGLILINEASKTVPLDSNIFPFTQIGNSEGLQI 485
GKIV+C SDDFSTSRTIKELVVQDAKA+GLILINEASKTVP+DSNIFPFTQIGNSEGLQI
Sbjct: 443 GKIVVCASDDFSTSRTIKELVVQDAKAVGLILINEASKTVPMDSNIFPFTQIGNSEGLQI 502
Query: 486 LEYINSTKNPTATILRTVEVRRLKPAPMVAYFSSRGPSPLTENILKPDITAPGVSILAAV 545
LEYINSTKNPTATILRTVEVRRLKPAP+VAYFSSRGPSPLTENILKPDI APGVSILAA+
Sbjct: 503 LEYINSTKNPTATILRTVEVRRLKPAPVVAYFSSRGPSPLTENILKPDIMAPGVSILAAI 562
Query: 546 VPKSDAESGPIGKKPSDYAMRSGTSMACPHVAGAAAFVKSVRHNWSSSMIKSALMTTATI 605
PKSDAESGPIGKKPS+YAMRSGTSM+CPHVAGAAAFVKSV HNWSSSMIKSALMTTAT+
Sbjct: 563 APKSDAESGPIGKKPSNYAMRSGTSMSCPHVAGAAAFVKSVYHNWSSSMIKSALMTTATL 622
Query: 606 YDNQRKFMRNSTNNPSNPHEMGAGEISPIKALNPGLVFESTNEDYLRFLCYYGYSNKIIR 665
YDNQRKFMRNSTNNPSNPHEMGAGEISPIKALNPGLV+ESTNEDYLRFLCYYGYSNKIIR
Sbjct: 623 YDNQRKFMRNSTNNPSNPHEMGAGEISPIKALNPGLVYESTNEDYLRFLCYYGYSNKIIR 682
Query: 666 SVSKQNFSCPKTSSEDLISSVNYPSISIGKLDRKQAA--KVIERTVTNVGAPNTTYIAKV 725
SVSKQNFSCPKTS E LISSVNYPSISI KLDRK A V+ERTVTNVGAP+ TYIAKV
Sbjct: 683 SVSKQNFSCPKTSKEYLISSVNYPSISISKLDRKVAGGKVVVERTVTNVGAPDATYIAKV 742
Query: 726 HSSEGLLVKVIPSKIVFSENVKKVTFKVSFYGKEARSGYNFGTITWRDSVHSVRTVFAVN 784
HSSEGL+VKV PSKIVF+E VKKVTFKVSFYGKE RSGYNFGTITWRD+ HSVRTVFAVN
Sbjct: 743 HSSEGLIVKVTPSKIVFTEKVKKVTFKVSFYGKEVRSGYNFGTITWRDTAHSVRTVFAVN 801
BLAST of Lcy01g013470 vs. NCBI nr
Match:
KAG7029593.1 (CO(2)-response secreted protease [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1408.7 bits (3645), Expect = 0.0e+00
Identity = 709/781 (90.78%), Postives = 744/781 (95.26%), Query Frame = 0
Query: 6 LLLLHLLLLPLSLSANPSSIATLQNLPLKHYVVYMGSGGSNYEEDDQTAAELDYLQLLSS 65
LLLLH LLP SLSA PS+IA LQNLPLKHYVVYMG G S+ EE ++TA ELD+LQLLSS
Sbjct: 11 LLLLH--LLPPSLSATPSAIAALQNLPLKHYVVYMGGGSSSNEEHEETAGELDFLQLLSS 70
Query: 66 VIP-RKEKERGSRDVVHQYHNAFKGFSAMLTEEEASSLSGVDGIVSVFPDPTLQLHTTRS 125
V P R+EKERGSRD++HQYH+AFKGFSAMLTEEEASSLSGVDGIVSVFPDPTLQLHTTRS
Sbjct: 71 VNPRRREKERGSRDLIHQYHHAFKGFSAMLTEEEASSLSGVDGIVSVFPDPTLQLHTTRS 130
Query: 126 WDFLDSITGLRPPTPLPPPHSYLSSTDVIVGVVDTGIWPESQSFNDEGLGEIPSKWKGVC 185
WDFLDSI+GLRPPTPLPPPHSY SS+DV+VG++DTGIWPESQSFNDEG+GEIPSKWKGVC
Sbjct: 131 WDFLDSISGLRPPTPLPPPHSYASSSDVVVGIIDTGIWPESQSFNDEGIGEIPSKWKGVC 190
Query: 186 MEAPDFKKSNCNRKLVGARYYNAIEPNGNDSRVGMPKGTPRDLLGHGTHTSSIAAGARVP 245
MEAPDFKKSNCNRKL+GARYYN +EPNGNDSR +PKGTPRD LGHGTHTSSIAAGARVP
Sbjct: 191 MEAPDFKKSNCNRKLIGARYYNVVEPNGNDSRGSVPKGTPRDSLGHGTHTSSIAAGARVP 250
Query: 246 NASYFDLARGTARGGGTVSTRIASYKVCAGVGCSGAAILKAIDDAVKDGVDIISISIGIG 305
NASYF LARGTARGGG STRIASYKVCAGVGCSGAAILKAID+AVKDGVDIISISIGIG
Sbjct: 251 NASYFGLARGTARGGGIPSTRIASYKVCAGVGCSGAAILKAIDEAVKDGVDIISISIGIG 310
Query: 306 SPLFQSDYLNDPIAIGAFHAQQMGVLVVCSGGNDGPDPNTVGNIAPWIFTVAASNIDRDF 365
SPLFQSDYLNDPIAIGAFHAQQMGVLVVCS GNDGPDPNTVGN+APWIFTVAASNIDRDF
Sbjct: 311 SPLFQSDYLNDPIAIGAFHAQQMGVLVVCSAGNDGPDPNTVGNVAPWIFTVAASNIDRDF 370
Query: 366 QSTVVLGNGKTFQGTAINLSNLTTSKTYPLVFGKDAAAKFTPISEARNCYPGSLDRSKVA 425
QSTVVLGNGKTFQGTAINLSNLT+SKTYPLVFGKDAAAKFTPISEARNCYPGSLDRSKVA
Sbjct: 371 QSTVVLGNGKTFQGTAINLSNLTSSKTYPLVFGKDAAAKFTPISEARNCYPGSLDRSKVA 430
Query: 426 GKIVICTSDDFSTSRTIKELVVQDAKAMGLILINEASKTVPLDSNIFPFTQIGNSEGLQI 485
GKIV+C SDDFSTSRTIKELVVQDAKA+GLILINEASKTVP+DSNIFPFTQIGNSEGLQI
Sbjct: 431 GKIVVCASDDFSTSRTIKELVVQDAKAVGLILINEASKTVPMDSNIFPFTQIGNSEGLQI 490
Query: 486 LEYINSTKNPTATILRTVEVRRLKPAPMVAYFSSRGPSPLTENILKPDITAPGVSILAAV 545
LEYINSTKNPTATILRTVEVRRLKPAP+VAYFSSRGPSPLTENILKPDI APGVSILAA+
Sbjct: 491 LEYINSTKNPTATILRTVEVRRLKPAPVVAYFSSRGPSPLTENILKPDIMAPGVSILAAI 550
Query: 546 VPKSDAESGPIGKKPSDYAMRSGTSMACPHVAGAAAFVKSVRHNWSSSMIKSALMTTATI 605
PKSDAESGPIGKKPS+YAMRSGTSM+CPHVAGAAAFVKSV HNWSSSMIKSALMTTAT+
Sbjct: 551 APKSDAESGPIGKKPSNYAMRSGTSMSCPHVAGAAAFVKSVYHNWSSSMIKSALMTTATL 610
Query: 606 YDNQRKFMRNSTNNPSNPHEMGAGEISPIKALNPGLVFESTNEDYLRFLCYYGYSNKIIR 665
YDNQRKFMRNSTNNPSNPHEMGAGEISPIKALNPGLV+ESTNEDYLRFLCYYGYSNKIIR
Sbjct: 611 YDNQRKFMRNSTNNPSNPHEMGAGEISPIKALNPGLVYESTNEDYLRFLCYYGYSNKIIR 670
Query: 666 SVSKQNFSCPKTSSEDLISSVNYPSISIGKLDRKQAA--KVIERTVTNVGAPNTTYIAKV 725
SVSKQNFSCPKTS E LISSVNYPSISI KLDRK A V+ERTVTNVGAP+ TYIAKV
Sbjct: 671 SVSKQNFSCPKTSKEYLISSVNYPSISISKLDRKVAGGKVVVERTVTNVGAPDATYIAKV 730
Query: 726 HSSEGLLVKVIPSKIVFSENVKKVTFKVSFYGKEARSGYNFGTITWRDSVHSVRTVFAVN 784
HSSEGL+VKV PSKIVF+E VKKVTFKVSFYGKEARSGYNFGTITWRD+ HSVRTVFAVN
Sbjct: 731 HSSEGLIVKVTPSKIVFTEKVKKVTFKVSFYGKEARSGYNFGTITWRDTAHSVRTVFAVN 789
BLAST of Lcy01g013470 vs. NCBI nr
Match:
XP_022997067.1 (CO(2)-response secreted protease-like [Cucurbita maxima])
HSP 1 Score: 1403.3 bits (3631), Expect = 0.0e+00
Identity = 704/781 (90.14%), Postives = 742/781 (95.01%), Query Frame = 0
Query: 6 LLLLHLLLLPLSLSANPSSIATLQNLPLKHYVVYMGSGGSNYEEDDQTAAELDYLQLLSS 65
LLLL L LLP SLSA PS+IA LQNLPLKHYVVYMG G S+ EE +Q A ELD+LQLLSS
Sbjct: 23 LLLLLLHLLPPSLSATPSAIAALQNLPLKHYVVYMGGGSSSNEEHEQRAGELDFLQLLSS 82
Query: 66 VIP-RKEKERGSRDVVHQYHNAFKGFSAMLTEEEASSLSGVDGIVSVFPDPTLQLHTTRS 125
V P R+EKERGSRD++HQYH+AFKGFSAMLTEEEASSLSG+DGIVSVFPDPTLQLHTTRS
Sbjct: 83 VNPRRREKERGSRDLIHQYHHAFKGFSAMLTEEEASSLSGIDGIVSVFPDPTLQLHTTRS 142
Query: 126 WDFLDSITGLRPPTPLPPPHSYLSSTDVIVGVVDTGIWPESQSFNDEGLGEIPSKWKGVC 185
WDFLDSI+GLRPPTPLPPPHSY SS+DV+VG++DTGIWPESQSFNDEG+GEIPSKWKGVC
Sbjct: 143 WDFLDSISGLRPPTPLPPPHSYASSSDVVVGIIDTGIWPESQSFNDEGIGEIPSKWKGVC 202
Query: 186 MEAPDFKKSNCNRKLVGARYYNAIEPNGNDSRVGMPKGTPRDLLGHGTHTSSIAAGARVP 245
MEAPDFKKSNCNRKL+GARYYN +EPNGNDS +PKGTPRD LGHGTHTSSIAAG+RVP
Sbjct: 203 MEAPDFKKSNCNRKLIGARYYNVVEPNGNDSHGSLPKGTPRDSLGHGTHTSSIAAGSRVP 262
Query: 246 NASYFDLARGTARGGGTVSTRIASYKVCAGVGCSGAAILKAIDDAVKDGVDIISISIGIG 305
NASYF LARGTARGGG STRIASYKVCAGVGCSGAAILKAIDDAVKDGVDIISISIGIG
Sbjct: 263 NASYFGLARGTARGGGIPSTRIASYKVCAGVGCSGAAILKAIDDAVKDGVDIISISIGIG 322
Query: 306 SPLFQSDYLNDPIAIGAFHAQQMGVLVVCSGGNDGPDPNTVGNIAPWIFTVAASNIDRDF 365
SPLFQSDYLNDPIAIGAFHAQQMGVLVVCS GNDGPDPNTVGN+APWIFTVAASNIDRDF
Sbjct: 323 SPLFQSDYLNDPIAIGAFHAQQMGVLVVCSAGNDGPDPNTVGNVAPWIFTVAASNIDRDF 382
Query: 366 QSTVVLGNGKTFQGTAINLSNLTTSKTYPLVFGKDAAAKFTPISEARNCYPGSLDRSKVA 425
QSTVVLGNGKTFQGTAINLSNLT+SKTYPLVFGKDAAAKFTP+SEARNCYPGSLDRSKVA
Sbjct: 383 QSTVVLGNGKTFQGTAINLSNLTSSKTYPLVFGKDAAAKFTPMSEARNCYPGSLDRSKVA 442
Query: 426 GKIVICTSDDFSTSRTIKELVVQDAKAMGLILINEASKTVPLDSNIFPFTQIGNSEGLQI 485
GKIV+C SDDFSTSRTIKELVVQDAKA+GLILINEASKTVP+DSNIFPFTQIGNSEGLQI
Sbjct: 443 GKIVVCASDDFSTSRTIKELVVQDAKAVGLILINEASKTVPMDSNIFPFTQIGNSEGLQI 502
Query: 486 LEYINSTKNPTATILRTVEVRRLKPAPMVAYFSSRGPSPLTENILKPDITAPGVSILAAV 545
LEYINSTKNPTATILRTVEVRRLKPAP+VAYFSSRGPSPLTENILKPDI APGVSILAA+
Sbjct: 503 LEYINSTKNPTATILRTVEVRRLKPAPVVAYFSSRGPSPLTENILKPDIMAPGVSILAAI 562
Query: 546 VPKSDAESGPIGKKPSDYAMRSGTSMACPHVAGAAAFVKSVRHNWSSSMIKSALMTTATI 605
PKSDAESGPIGKKPS+YAMRSGTSM+CPHVAGAAAFVKSV HNWSSSMIKSALMTTAT+
Sbjct: 563 APKSDAESGPIGKKPSNYAMRSGTSMSCPHVAGAAAFVKSVYHNWSSSMIKSALMTTATL 622
Query: 606 YDNQRKFMRNSTNNPSNPHEMGAGEISPIKALNPGLVFESTNEDYLRFLCYYGYSNKIIR 665
YDNQRKFMRNSTNNPSNPHEMGAGEISPIKALNPGLV+ESTNEDYLRFLCYYGYSNKIIR
Sbjct: 623 YDNQRKFMRNSTNNPSNPHEMGAGEISPIKALNPGLVYESTNEDYLRFLCYYGYSNKIIR 682
Query: 666 SVSKQNFSCPKTSSEDLISSVNYPSISIGKLDRKQAA--KVIERTVTNVGAPNTTYIAKV 725
SVSKQNFSCPKTS E LISS+NYPSISI KLDRK AA V+ERTVTNVGAP+ TYIAKV
Sbjct: 683 SVSKQNFSCPKTSKEYLISSINYPSISISKLDRKVAAGKVVVERTVTNVGAPDATYIAKV 742
Query: 726 HSSEGLLVKVIPSKIVFSENVKKVTFKVSFYGKEARSGYNFGTITWRDSVHSVRTVFAVN 784
HSSEGL+VKV PSKI F+E VKKVTFKVSFYGKEAR+GYNFGTITWRD+ HSVRTVFAVN
Sbjct: 743 HSSEGLIVKVTPSKITFTEKVKKVTFKVSFYGKEARNGYNFGTITWRDTAHSVRTVFAVN 802
BLAST of Lcy01g013470 vs. NCBI nr
Match:
XP_023547000.1 (CO(2)-response secreted protease-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1401.7 bits (3627), Expect = 0.0e+00
Identity = 707/782 (90.41%), Postives = 745/782 (95.27%), Query Frame = 0
Query: 6 LLLLHLLLLPLSLSANPSSIATLQNLPLKHYVVYMGSG-GSNYEEDDQTAAELDYLQLLS 65
LLLL L LLP SLSA PS+IA LQNLPLKHYVVYMG G S+ EE ++TA ELD+LQLLS
Sbjct: 8 LLLLLLHLLPPSLSATPSAIAALQNLPLKHYVVYMGGGSSSSNEEHEETAGELDFLQLLS 67
Query: 66 SVIP-RKEKERGSRDVVHQYHNAFKGFSAMLTEEEASSLSGVDGIVSVFPDPTLQLHTTR 125
SV P R+EKERGSRD++HQYH+AFKGFSAMLTEEEASSLSG+DGIVSVFPDPTLQLHTTR
Sbjct: 68 SVNPRRREKERGSRDLIHQYHHAFKGFSAMLTEEEASSLSGIDGIVSVFPDPTLQLHTTR 127
Query: 126 SWDFLDSITGLRPPTPLPPPHSYLSSTDVIVGVVDTGIWPESQSFNDEGLGEIPSKWKGV 185
SWDFL+SI+GLRPPTPLPPPHSY SS+DV+VG++DTGIWPESQSFNDEG+GEIPSKWKGV
Sbjct: 128 SWDFLNSISGLRPPTPLPPPHSYASSSDVVVGIIDTGIWPESQSFNDEGIGEIPSKWKGV 187
Query: 186 CMEAPDFKKSNCNRKLVGARYYNAIEPNGNDSRVGMPKGTPRDLLGHGTHTSSIAAGARV 245
CMEAPDFKKSNCNRKL+GARYYN +EPNGNDSR +PKGTPRD LGHGTHTSSIAAGARV
Sbjct: 188 CMEAPDFKKSNCNRKLIGARYYNVVEPNGNDSRGSVPKGTPRDSLGHGTHTSSIAAGARV 247
Query: 246 PNASYFDLARGTARGGGTVSTRIASYKVCAGVGCSGAAILKAIDDAVKDGVDIISISIGI 305
PNASYF LARGTARGGG STRIASYKVCAGVGCSGAAILKAIDDAVKDGVDIISISIGI
Sbjct: 248 PNASYFGLARGTARGGGIPSTRIASYKVCAGVGCSGAAILKAIDDAVKDGVDIISISIGI 307
Query: 306 GSPLFQSDYLNDPIAIGAFHAQQMGVLVVCSGGNDGPDPNTVGNIAPWIFTVAASNIDRD 365
GSPLFQSDYLNDPIAIGAFHAQQMGVLVVCS GNDGPDPNTVGN+APWIFTVAASNIDRD
Sbjct: 308 GSPLFQSDYLNDPIAIGAFHAQQMGVLVVCSAGNDGPDPNTVGNVAPWIFTVAASNIDRD 367
Query: 366 FQSTVVLGNGKTFQGTAINLSNLTTSKTYPLVFGKDAAAKFTPISEARNCYPGSLDRSKV 425
FQSTVVLGNGKTFQGTAINLSNLT+SKTYPLVFGKDAAAKFTPISEARNCYPGSLDRSKV
Sbjct: 368 FQSTVVLGNGKTFQGTAINLSNLTSSKTYPLVFGKDAAAKFTPISEARNCYPGSLDRSKV 427
Query: 426 AGKIVICTSDDFSTSRTIKELVVQDAKAMGLILINEASKTVPLDSNIFPFTQIGNSEGLQ 485
AGKIV+C SDDFSTSRTIKELVVQDAKA+GLILI+EASKTVP+DSNIFPFTQIGNSEGLQ
Sbjct: 428 AGKIVVCASDDFSTSRTIKELVVQDAKAVGLILISEASKTVPMDSNIFPFTQIGNSEGLQ 487
Query: 486 ILEYINSTKNPTATILRTVEVRRLKPAPMVAYFSSRGPSPLTENILKPDITAPGVSILAA 545
ILEYINSTKNPTATILRTVEVRRLKPAP+VAYFSSRGPSPLTENILKPDI APGVSILAA
Sbjct: 488 ILEYINSTKNPTATILRTVEVRRLKPAPVVAYFSSRGPSPLTENILKPDIMAPGVSILAA 547
Query: 546 VVPKSDAESGPIGKKPSDYAMRSGTSMACPHVAGAAAFVKSVRHNWSSSMIKSALMTTAT 605
+ PKSDAESGPIGKKPS+YAMRSGTSM+CPHVAGAAAFVKSV HNWSSSMIKSALMTTAT
Sbjct: 548 IAPKSDAESGPIGKKPSNYAMRSGTSMSCPHVAGAAAFVKSVYHNWSSSMIKSALMTTAT 607
Query: 606 IYDNQRKFMRNSTNNPSNPHEMGAGEISPIKALNPGLVFESTNEDYLRFLCYYGYSNKII 665
+YDNQRKFMRNSTNNPSNPHEMGAGEISPIKALNPGLV+ESTNEDYLRFLCYYGYSNKII
Sbjct: 608 LYDNQRKFMRNSTNNPSNPHEMGAGEISPIKALNPGLVYESTNEDYLRFLCYYGYSNKII 667
Query: 666 RSVSKQNFSCPKTSSEDLISSVNYPSISIGKLDRKQAA--KVIERTVTNVGAPNTTYIAK 725
RSVSKQNFSCPKTS E LISSVNYPSISI KLDRK AA V+ERTVTNVGAP+ TYIAK
Sbjct: 668 RSVSKQNFSCPKTSKEYLISSVNYPSISISKLDRKVAAGKVVVERTVTNVGAPDATYIAK 727
Query: 726 VHSSEGLLVKVIPSKIVFSENVKKVTFKVSFYGKEARSGYNFGTITWRDSVHSVRTVFAV 784
VHSSEGL+VKV PSKIVF+E VKKVTFKVSFYGKEAR+GYNFGTITWRD+ HSVRTVFAV
Sbjct: 728 VHSSEGLIVKVTPSKIVFTEKVKKVTFKVSFYGKEARNGYNFGTITWRDTAHSVRTVFAV 787
BLAST of Lcy01g013470 vs. NCBI nr
Match:
KAG6598653.1 (CO(2)-response secreted protease, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1394.4 bits (3608), Expect = 0.0e+00
Identity = 701/774 (90.57%), Postives = 736/774 (95.09%), Query Frame = 0
Query: 4 ISLLLLHLLLLPLSLSANPSSIATLQNLPLKHYVVYMGSGGSNYEEDDQTAAELDYLQLL 63
+ LLLL L LLP SLSA PS+IA LQNLPLKHYVVYMG G S+ EE ++TA ELD+LQLL
Sbjct: 8 LHLLLLLLHLLPPSLSATPSAIAALQNLPLKHYVVYMGGGSSSNEEHEETAGELDFLQLL 67
Query: 64 SSVIP-RKEKERGSRDVVHQYHNAFKGFSAMLTEEEASSLSGVDGIVSVFPDPTLQLHTT 123
SSV P R+EKERGSRD++HQYH+AFKGFSAMLTEEEASSLSGVDGIVSVFPDPTLQLHTT
Sbjct: 68 SSVNPRRREKERGSRDLIHQYHHAFKGFSAMLTEEEASSLSGVDGIVSVFPDPTLQLHTT 127
Query: 124 RSWDFLDSITGLRPPTPLPPPHSYLSSTDVIVGVVDTGIWPESQSFNDEGLGEIPSKWKG 183
RSWDFLDSI+GLRPPTPLPPPHSY SS+DV+VG++DTGIWPESQSFNDEG+GEIPSKWKG
Sbjct: 128 RSWDFLDSISGLRPPTPLPPPHSYASSSDVVVGIIDTGIWPESQSFNDEGIGEIPSKWKG 187
Query: 184 VCMEAPDFKKSNCNRKLVGARYYNAIEPNGNDSRVGMPKGTPRDLLGHGTHTSSIAAGAR 243
VCMEAPDFKKSNCNRKL+GARYYN +EPNGNDSR +PKGTPRD LGHGTHTSSIAAGAR
Sbjct: 188 VCMEAPDFKKSNCNRKLIGARYYNVVEPNGNDSRGSVPKGTPRDSLGHGTHTSSIAAGAR 247
Query: 244 VPNASYFDLARGTARGGGTVSTRIASYKVCAGVGCSGAAILKAIDDAVKDGVDIISISIG 303
VPNASYF LARGTARGGG STRIASYKVCAGVGCSGAAILKAIDDAVKDGVDIISISIG
Sbjct: 248 VPNASYFGLARGTARGGGIPSTRIASYKVCAGVGCSGAAILKAIDDAVKDGVDIISISIG 307
Query: 304 IGSPLFQSDYLNDPIAIGAFHAQQMGVLVVCSGGNDGPDPNTVGNIAPWIFTVAASNIDR 363
IGSPLFQSDYLNDPIAIGAFHAQQMGVLVVCS GNDGPDPNTVGN+APWIFTVAASNIDR
Sbjct: 308 IGSPLFQSDYLNDPIAIGAFHAQQMGVLVVCSAGNDGPDPNTVGNVAPWIFTVAASNIDR 367
Query: 364 DFQSTVVLGNGKTFQGTAINLSNLTTSKTYPLVFGKDAAAKFTPISEARNCYPGSLDRSK 423
DFQSTVVLGNGKTFQGTAINLSNLT+SKTYPLVFGKDAAAKFTPISEARNCYPGSLDRSK
Sbjct: 368 DFQSTVVLGNGKTFQGTAINLSNLTSSKTYPLVFGKDAAAKFTPISEARNCYPGSLDRSK 427
Query: 424 VAGKIVICTSDDFSTSRTIKELVVQDAKAMGLILINEASKTVPLDSNIFPFTQIGNSEGL 483
VAGKIV+C SDDFSTSRTIKELVVQDAKA+GLILINEASKTVP+DSNIFPFTQIGNSEGL
Sbjct: 428 VAGKIVVCASDDFSTSRTIKELVVQDAKAVGLILINEASKTVPMDSNIFPFTQIGNSEGL 487
Query: 484 QILEYINSTKNPTATILRTVEVRRLKPAPMVAYFSSRGPSPLTENILKPDITAPGVSILA 543
QILEYINSTKNPTATILRTVEVRRLKPAP+VAYFSSRGPSPLTENILKPDI APGVSILA
Sbjct: 488 QILEYINSTKNPTATILRTVEVRRLKPAPVVAYFSSRGPSPLTENILKPDIMAPGVSILA 547
Query: 544 AVVPKSDAESGPIGKKPSDYAMRSGTSMACPHVAGAAAFVKSVRHNWSSSMIKSALMTTA 603
A+ PKSDAESGPIGKKPS+YAMRSGTSM+CPHVAGAAAFVKSV HNWSSSMIKSALMTTA
Sbjct: 548 AIAPKSDAESGPIGKKPSNYAMRSGTSMSCPHVAGAAAFVKSVYHNWSSSMIKSALMTTA 607
Query: 604 TIYDNQRKFMRNSTNNPSNPHEMGAGEISPIKALNPGLVFESTNEDYLRFLCYYGYSNKI 663
T+YDNQRKFMRNSTNNPSNPHEMGAGEISPIKALNPGLV+ESTNEDYLRFLCYYGYSNKI
Sbjct: 608 TLYDNQRKFMRNSTNNPSNPHEMGAGEISPIKALNPGLVYESTNEDYLRFLCYYGYSNKI 667
Query: 664 IRSVSKQNFSCPKTSSEDLISSVNYPSISIGKLDRKQAA--KVIERTVTNVGAPNTTYIA 723
IRSVSKQNFSCPKTS E LISSVNYPSISI KLDRK A V+ERTVTNVGAP+ TYIA
Sbjct: 668 IRSVSKQNFSCPKTSKEYLISSVNYPSISISKLDRKVAGGKVVVERTVTNVGAPDATYIA 727
Query: 724 KVHSSEGLLVKVIPSKIVFSENVKKVTFKVSFYGKEARSGYNFGTITWRDSVHS 775
KVHSSEGL+VKV PSKIVF+E VKKVTFKVSFYGKEARSGYNFGTITWRD+ HS
Sbjct: 728 KVHSSEGLIVKVTPSKIVFTEKVKKVTFKVSFYGKEARSGYNFGTITWRDTAHS 781
BLAST of Lcy01g013470 vs. TAIR 10
Match:
AT1G20160.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 636.3 bits (1640), Expect = 3.2e-182
Identity = 350/754 (46.42%), Postives = 475/754 (63.00%), Query Frame = 0
Query: 36 YVVYMGSGGSNYEEDDQTAAELDYLQLLSSVIPRKEKERGSRDVVHQYHNAFKGFSAMLT 95
Y+VYMGS S AA + Q+L + + +R + D++H Y + F GF+A LT
Sbjct: 36 YIVYMGSASS--------AANANRAQILINTM----FKRRANDLLHTYKHGFSGFAARLT 95
Query: 96 EEEASSLSGVDGIVSVFPDPTLQLHTTRSWDFLDSITGLRPPTPLPPPHSYLSSTDVIVG 155
EEA ++ G+VSVFPDP QLHTT SWDFL T ++ + PP + S D IVG
Sbjct: 96 AEEAKVIAKKPGVVSVFPDPHFQLHTTHSWDFLKYQTSVKVDSG-PPSSASDGSYDSIVG 155
Query: 156 VVDTGIWPESQSFNDEGLGEIPSKWKGVCMEAPDFKKSNCNRKLVGARYYNAIEPNGNDS 215
++DTGIWPES+SFND+ +G IPS+WKG CMEA DFK SNCNRK++GARYY P+ +
Sbjct: 156 ILDTGIWPESESFNDKDMGPIPSRWKGTCMEAKDFKSSNCNRKIIGARYYK--NPDDDSE 215
Query: 216 RVGMPKGTPRDLLGHGTHTSSIAAGARVPNASYFDLARGTARGGGTVSTRIASYKVCAGV 275
T RD++GHG+H SS AG+ V NASY+ +A GTA+ GG+ + RIA YKVC
Sbjct: 216 YY-----TTRDVIGHGSHVSSTIAGSAVENASYYGVASGTAK-GGSQNARIAMYKVCNPG 275
Query: 276 GCSGAAILKAIDDAVKDGVDIISISIGIGSPLFQSDYLN-DPIAIGAFHAQQMGVLVVCS 335
GC+G++IL A DDA+ DGVD++S+S +G+P + LN DPIAIGAFHA + G+LV+CS
Sbjct: 276 GCTGSSILAAFDDAIADGVDVLSLS--LGAPAYARIDLNTDPIAIGAFHAVEQGILVICS 335
Query: 336 GGNDGPDPNTVGNIAPWIFTVAASNIDRDFQSTVVLGNGKTFQGTAINLSNLTTSKTYPL 395
GNDGPD TV N APWI TVAA+ IDRDF+S VVLG K +G I+ SN++ S YPL
Sbjct: 336 AGNDGPDGGTVTNTAPWIMTVAANTIDRDFESDVVLGGNKVIKGEGIHFSNVSKSPVYPL 395
Query: 396 VFGKDAAAKFTPISEARNCYPGSLDRSKVAGKIVICTSDDFSTSRTIKELVVQDAKAMGL 455
+ GK A + AR C SLD+ KV GKIV+C + S + V+ G
Sbjct: 396 IHGKSAKSADASEGSARACDSDSLDQEKVKGKIVLCENVGGSYYASSARDEVKSKGGTGC 455
Query: 456 ILINEASKTVPLDSNIFPFTQIGNSEGLQILEYINSTKNPTATILRTVEVRRLKPAPMVA 515
+ +++ ++ V FP T I + E +I Y+NSTK+P ATIL T V + PAP VA
Sbjct: 456 VFVDDRTRAVASAYGSFPTTVIDSKEAAEIFSYLNSTKDPVATILPTATVEKFTPAPAVA 515
Query: 516 YFSSRGPSPLTENILKPDITAPGVSILAAVVPKSDAESGPIGKKPSDYAMRSGTSMACPH 575
YFSSRGPS LT +ILKPDITAPGVSILAA +D+ GK S Y + SGTSMA PH
Sbjct: 516 YFSSRGPSSLTRSILKPDITAPGVSILAAWT-GNDSSISLEGKPASQYNVISGTSMAAPH 575
Query: 576 VAGAAAFVKSVRHNWSSSMIKSALMTTATIYDNQRKFMRNSTNNPSNPHEMGAGEISPIK 635
V+ A+ +KS W S I+SA+MTTAT +N + + T + P++ GAGE+S
Sbjct: 576 VSAVASLIKSQHPTWGPSAIRSAIMTTATQTNNDKGLITTETGATATPYDSGAGELSSTA 635
Query: 636 ALNPGLVFESTNEDYLRFLCYYGYSNKIIRSVSK---QNFSCPKTSSEDLISSVNYPSIS 695
++ PGLV+E+T DYL FLCYYGY+ I+++SK +NF+CP S+ DLIS++NYPSI
Sbjct: 636 SMQPGLVYETTETDYLNFLCYYGYNVTTIKAMSKAFPENFTCPADSNLDLISTINYPSIG 695
Query: 696 IGKLDRKQAAKVIERTVTNVGAP-NTTYIAKVHSSEGLLVKVIPSKIVFSENVKKVTFKV 755
I + +K + RTVTNVG Y V + G ++V P K+ F+++ +K+T++V
Sbjct: 696 ISGF-KGNGSKTVTRTVTNVGEDGEAVYTVSVETPPGFNIQVTPEKLQFTKDGEKLTYQV 755
Query: 756 SFYGKEARSGYNFGTITWRDSVHSVRTVFAVNVE 785
+ FG +TW ++ + VR+ ++ E
Sbjct: 756 IVSATASLKQDVFGALTWSNAKYKVRSPIVISSE 764
BLAST of Lcy01g013470 vs. TAIR 10
Match:
AT1G20160.2 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 627.9 bits (1618), Expect = 1.1e-179
Identity = 339/717 (47.28%), Postives = 459/717 (64.02%), Query Frame = 0
Query: 73 ERGSRDVVHQYHNAFKGFSAMLTEEEASSLSGVDGIVSVFPDPTLQLHTTRSWDFLDSIT 132
+R + D++H Y + F GF+A LT EEA ++ G+VSVFPDP QLHTT SWDFL T
Sbjct: 22 KRRANDLLHTYKHGFSGFAARLTAEEAKVIAKKPGVVSVFPDPHFQLHTTHSWDFLKYQT 81
Query: 133 GLRPPTPLPPPHSYLSSTDVIVGVVDTGIWPESQSFNDEGLGEIPSKWKGVCMEAPDFKK 192
++ + PP + S D IVG++DTGIWPES+SFND+ +G IPS+WKG CMEA DFK
Sbjct: 82 SVKVDSG-PPSSASDGSYDSIVGILDTGIWPESESFNDKDMGPIPSRWKGTCMEAKDFKS 141
Query: 193 SNCNRKLVGARYYNAIEPNGNDSRVGMPKGTPRDLLGHGTHTSSIAAGARVPNASYFDLA 252
SNCNRK++GARYY P+ + T RD++GHG+H SS AG+ V NASY+ +A
Sbjct: 142 SNCNRKIIGARYYK--NPDDDSEYY-----TTRDVIGHGSHVSSTIAGSAVENASYYGVA 201
Query: 253 RGTARGGGTVSTRIASYKVCAGVGCSGAAILKAIDDAVKDGVDIISISIGIGSPLFQSDY 312
GTA+ GG+ + RIA YKVC GC+G++IL A DDA+ DGVD++S+S +G+P +
Sbjct: 202 SGTAK-GGSQNARIAMYKVCNPGGCTGSSILAAFDDAIADGVDVLSLS--LGAPAYARID 261
Query: 313 LN-DPIAIGAFHAQQMGVLVVCSGGNDGPDPNTVGNIAPWIFTVAASNIDRDFQSTVVLG 372
LN DPIAIGAFHA + G+LV+CS GNDGPD TV N APWI TVAA+ IDRDF+S VVLG
Sbjct: 262 LNTDPIAIGAFHAVEQGILVICSAGNDGPDGGTVTNTAPWIMTVAANTIDRDFESDVVLG 321
Query: 373 NGKTFQGTAINLSNLTTSKTYPLVFGKDAAAKFTPISEARNCYPGSLDRSKVAGKIVICT 432
K +G I+ SN++ S YPL+ GK A + AR C SLD+ KV GKIV+C
Sbjct: 322 GNKVIKGEGIHFSNVSKSPVYPLIHGKSAKSADASEGSARACDSDSLDQEKVKGKIVLCE 381
Query: 433 SDDFSTSRTIKELVVQDAKAMGLILINEASKTVPLDSNIFPFTQIGNSEGLQILEYINST 492
+ S + V+ G + +++ ++ V FP T I + E +I Y+NST
Sbjct: 382 NVGGSYYASSARDEVKSKGGTGCVFVDDRTRAVASAYGSFPTTVIDSKEAAEIFSYLNST 441
Query: 493 KNPTATILRTVEVRRLKPAPMVAYFSSRGPSPLTENILKPDITAPGVSILAAVVPKSDAE 552
K+P ATIL T V + PAP VAYFSSRGPS LT +ILKPDITAPGVSILAA +D+
Sbjct: 442 KDPVATILPTATVEKFTPAPAVAYFSSRGPSSLTRSILKPDITAPGVSILAAWT-GNDSS 501
Query: 553 SGPIGKKPSDYAMRSGTSMACPHVAGAAAFVKSVRHNWSSSMIKSALMTTATIYDNQRKF 612
GK S Y + SGTSMA PHV+ A+ +KS W S I+SA+MTTAT +N +
Sbjct: 502 ISLEGKPASQYNVISGTSMAAPHVSAVASLIKSQHPTWGPSAIRSAIMTTATQTNNDKGL 561
Query: 613 MRNSTNNPSNPHEMGAGEISPIKALNPGLVFESTNEDYLRFLCYYGYSNKIIRSVSK--- 672
+ T + P++ GAGE+S ++ PGLV+E+T DYL FLCYYGY+ I+++SK
Sbjct: 562 ITTETGATATPYDSGAGELSSTASMQPGLVYETTETDYLNFLCYYGYNVTTIKAMSKAFP 621
Query: 673 QNFSCPKTSSEDLISSVNYPSISIGKLDRKQAAKVIERTVTNVGAP-NTTYIAKVHSSEG 732
+NF+CP S+ DLIS++NYPSI I + +K + RTVTNVG Y V + G
Sbjct: 622 ENFTCPADSNLDLISTINYPSIGISGF-KGNGSKTVTRTVTNVGEDGEAVYTVSVETPPG 681
Query: 733 LLVKVIPSKIVFSENVKKVTFKVSFYGKEARSGYNFGTITWRDSVHSVRTVFAVNVE 785
++V P K+ F+++ +K+T++V + FG +TW ++ + VR+ ++ E
Sbjct: 682 FNIQVTPEKLQFTKDGEKLTYQVIVSATASLKQDVFGALTWSNAKYKVRSPIVISSE 725
BLAST of Lcy01g013470 vs. TAIR 10
Match:
AT1G20150.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 563.9 bits (1452), Expect = 2.0e-160
Identity = 322/765 (42.09%), Postives = 470/765 (61.44%), Query Frame = 0
Query: 36 YVVYMGSGGSNYEEDDQTAAELDYLQLLSSVIPRKEKERGSRDVVHQYHNAFKGFSAMLT 95
Y++YMG+ S+ D+ D+++LLSS++ +R + +H+Y + F GF+A L+
Sbjct: 33 YIIYMGAASSDGSTDN------DHVELLSSLL-----QRSGKTPMHRYKHGFSGFAAHLS 92
Query: 96 EEEASSLSGVDGIVSVFPDPTLQLHTTRSWDFLDSITGLRPP--TPLP-PPHSYLSSTDV 155
E+EA ++ G++SVFPD LQLHTTRSWDFL + R T + S + D
Sbjct: 93 EDEAHLIAKQPGVLSVFPDQMLQLHTTRSWDFLVQESYQRDTYFTEMNYEQESEMHEGDT 152
Query: 156 IVGVVDTGIWPESQSFNDEGLGEIPSKWKGVCMEAPDFKKSN--CNRKLVGARYYNA--- 215
I+G +D+GIWPE+QSFND +G +P KWKG CM + + CNRKL+GARYYN+
Sbjct: 153 IIGFLDSGIWPEAQSFNDRHMGPVPEKWKGTCMRGKKTQPDSFRCNRKLIGARYYNSSFF 212
Query: 216 IEPNGNDSRVGMPKGTPRDLLGHGTHTSSIAAGARVPNASYFDLARGTARGGGTVSTRIA 275
++P+ TPRD LGHGTH +SIAAG + NASY+ LA G R GG+ S+RIA
Sbjct: 213 LDPDYE---------TPRDFLGHGTHVASIAAGQIIANASYYGLASGIMR-GGSPSSRIA 272
Query: 276 SYKVCAGVGCSGAAILKAIDDAVKDGVDIISISIGIGSPLFQSDYLNDPIAIGAFHAQQM 335
Y+ C+ +GC G++IL A DDA+ DGVD+ISIS+G L+ + L DP++IG+FHA +
Sbjct: 273 MYRACSLLGCRGSSILAAFDDAIADGVDVISISMG----LWPDNLLEDPLSIGSFHAVER 332
Query: 336 GVLVVCSGGNDGPDPNTVGNIAPWIFTVAASNIDRDFQSTVVLG--NGKTFQGTAINLSN 395
G+ VVCS GN GP +V N APW+ TVAAS IDR F+S ++LG + +G IN++N
Sbjct: 333 GITVVCSVGNSGPSSQSVFNAAPWMITVAASTIDRGFESNILLGGDENRLIEGFGINIAN 392
Query: 396 LTTSKTYPLVFGKDAAAKFTPISEARNCYPGSLDRSKVAGKIVICTSDDFSTSRTIKELV 455
+ ++ YPL+ + A ARNC P +LD++ V GKIV+C SD + K
Sbjct: 393 IDKTQAYPLIHARSAKKIDANEEAARNCAPDTLDQTIVKGKIVVCDSDLDNQVIQWKSDE 452
Query: 456 VQDAKAMGLILINEASKTVPLDSNIFPFTQIGNSEGLQILEYINSTKNPTATILRTVEVR 515
V+ +G++L+++ S + F T I +G+QI+ YINST+ P ATI+ T
Sbjct: 453 VKRLGGIGMVLVDDESMDLSFIDPSFLVTIIKPEDGIQIMSYINSTREPIATIMPTRSRT 512
Query: 516 RLKPAPMVAYFSSRGPSPLTENILKPDITAPGVSILAAVVPKSDAESGPIGKKPSDYAMR 575
AP + FSSRGP LT +ILKPDI APGV+ILA+ + D + P GK P + +
Sbjct: 513 GHMLAPSIPSFSSRGPYLLTRSILKPDIAAPGVNILASWL-VGDRNAAPEGKPPPLFNIE 572
Query: 576 SGTSMACPHVAGAAAFVKSVRHNWSSSMIKSALMTTATIYDNQRKFMRNSTNNPSNPHEM 635
SGTSM+CPHV+G AA +KS +WS + I+SA+MTTA N + T + P++
Sbjct: 573 SGTSMSCPHVSGIAARLKSRYPSWSPAAIRSAIMTTAVQMTNTGSHITTETGEKATPYDF 632
Query: 636 GAGEISPIKALNPGLVFESTNEDYLRFLCYYGYSNKIIRSVSK---QNFSCPKTSSEDLI 695
GAG+++ +PGL++E+ + DYL FL YYG+++ I+ +S Q F+CP+ S+ I
Sbjct: 633 GAGQVTIFGPSSPGLIYETNHMDYLNFLGYYGFTSDQIKKISNRIPQGFACPEQSNRGDI 692
Query: 696 SSVNYPSISIGKLDRKQAAKVIERTVTNV-----GAPNTTYIAKVHSSEGLLVKVIPSKI 755
S++NYPSISI + K++ +V RTVTNV G +T Y + + EGLLV+VIP ++
Sbjct: 693 SNINYPSISISNFNGKESRRV-SRTVTNVASRLIGDEDTVYTVSIDAPEGLLVRVIPRRL 752
Query: 756 VFSENVKKVTFKVSFYGKEA-RSGYNFGTITWRDSVHSVRTVFAV 782
F + K++++V F FG+ITW + +++VR+ F V
Sbjct: 753 HFRKIGDKLSYQVIFSSTTTILKDDAFGSITWSNGMYNVRSPFVV 770
BLAST of Lcy01g013470 vs. TAIR 10
Match:
AT5G59100.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 498.0 bits (1281), Expect = 1.3e-140
Identity = 310/756 (41.01%), Postives = 426/756 (56.35%), Query Frame = 0
Query: 36 YVVYMGSGGSNYEEDDQTAAELDYLQLLSSVIPRKEKERGSRDVVHQYHNAFKGFSAMLT 95
Y+VY+GS S E + D++ +L + E +V Y +F GF+A LT
Sbjct: 35 YIVYLGSLPSREEYTPMS----DHMSILQEITGESLIE---NRLVRSYKKSFNGFAARLT 94
Query: 96 EEEASSLSGVDGIVSVFPDPTLQLHTTRSWDFLDSITGLRPPTPLPPPHSYLSSTDVIVG 155
E E L+G++ +VSVFP L+L TT SW+F+ G++ + +D I+G
Sbjct: 95 ESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGIK------TKRTRSIESDTIIG 154
Query: 156 VVDTGIWPESQSFNDEGLGEIPSKWKGVCMEAPDFKKSNCNRKLVGARYYNAIEPNGNDS 215
V+D+GI+PES SF+D+G G P KWKG C +F CN K++GAR Y A + N
Sbjct: 155 VIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNF---TCNNKVIGARDYTA-KSKANQ- 214
Query: 216 RVGMPKGTPRDLLGHGTHTSSIAAGARVPNASYFDLARGTARGGGTVSTRIASYKVCAGV 275
T RD GHGTHT+SIAAG V N++++ L GTAR GG + RIA YKVC
Sbjct: 215 -------TARDYSGHGTHTASIAAGNAVANSNFYGLGNGTAR-GGVPAARIAVYKVCDNE 274
Query: 276 GCSGAAILKAIDDAVKDGVDIISISIGIGS-PLFQSDYLNDPIAIGAFHAQQMGVLVVCS 335
GC G A++ A DDA+ DGVD+ISISI + + P F+ DPIAIGAFHA +GVL V +
Sbjct: 275 GCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFE----EDPIAIGAFHAMAVGVLTVNA 334
Query: 336 GGNDGPDPNTVGNIAPWIFTVAASNIDRDFQSTVVLGNGKTFQGTAINLSNLTTSKTYPL 395
GN+GP +TV + APW+F+VAAS +R F + VVLG+GK G ++N ++ YPL
Sbjct: 335 AGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDM-NGTNYPL 394
Query: 396 VFGKDAAAKFTPISEARNCYPGSLDRSKVAGKIVICTSDDFSTSRTIKELVVQDAKAMGL 455
V+GK AA + +AR C P LD V GKIV+C S T + Q A+G
Sbjct: 395 VYGKSAALSTCSVDKARLCEPKCLDGKLVKGKIVLCDS-------TKGLIEAQKLGAVGS 454
Query: 456 ILINEASKTVPLDSNIFPFTQIGNSEGLQILEYINSTKNPTATILRTVEVRRLKPAPMVA 515
I+ N + S FP + + N + ++ Y+NSTKNP AT+L++ E+ + AP+VA
Sbjct: 455 IVKNPEPDRAFIRS--FPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQR-APLVA 514
Query: 516 YFSSRGPSPLTENILKPDITAPGVSILAAVVPKSDAESGPIGKKPSDYAMRSGTSMACPH 575
FSSRGPS + +ILKPDITAPGV ILAA P S + Y++ SGTSMACPH
Sbjct: 515 SFSSRGPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPH 574
Query: 576 VAGAAAFVKSVRHNWSSSMIKSALMTTATIYDNQRKFMRNSTNNPSNPHEMGAGEISPIK 635
VAG AA+VK+ WS SMI+SA+MTTA + + S G+G + PI
Sbjct: 575 VAGVAAYVKTFHPQWSPSMIQSAIMTTAW------PMNASGSGFVSTEFAYGSGHVDPID 634
Query: 636 ALNPGLVFESTNEDYLRFLCYYGYSNKIIRSVSKQNFSCPKTSSEDLISSVNYPSISIGK 695
A+NPGLV+E T D++ FLC Y++ +R +S N +C K S+ L ++NYP++S
Sbjct: 635 AINPGLVYELTKADHINFLCGLNYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKV 694
Query: 696 LDRKQAAKVIERTVTNVGAPNTTYIAKVHSSEG--LLVKVIPSKIVFSENVKKVTFKVSF 755
K +RTVTNVG +TY AKV G L +KV P + +K +F V+
Sbjct: 695 SGTKPFNITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTV 737
Query: 756 YGKEARSGYNFGT-------ITWRDSVHSVRTVFAV 782
S + GT + W D H+VR+ V
Sbjct: 755 ------SSDSIGTKQPVSANLIWSDGTHNVRSPIIV 737
BLAST of Lcy01g013470 vs. TAIR 10
Match:
AT5G59090.2 (subtilase 4.12 )
HSP 1 Score: 496.9 bits (1278), Expect = 3.0e-140
Identity = 316/789 (40.05%), Postives = 448/789 (56.78%), Query Frame = 0
Query: 1 MASISLLLLHLLLLPLSLSANPSSIATLQNLPLKHYVVYMGSGGSNYEEDDQTAAELDYL 60
+A+ + L LL+L L SS++ + + + Y+VYMGS S + + D++
Sbjct: 4 LAASTCLYSWLLVLLL------SSVSAIIDEDTQVYIVYMGSLSSRADYIPTS----DHM 63
Query: 61 QLLSSVIPRKEKERGSRDVVHQYHNAFKGFSAMLTEEEASSLSGVDGIVSVFPDPTLQLH 120
+L V E +V Y +F GF+A LTE E + ++ ++G+VSVFP+ LQLH
Sbjct: 64 SILQQVTGESSIE---GRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLH 123
Query: 121 TTRSWDFLDSITGLRPPTPLPPPHSYLSSTDVIVGVVDTGIWPESQSFNDEGLGEIPSKW 180
TT SWDF+ G L +D I+GV+DTGIWPES+SF+D+G G P KW
Sbjct: 124 TTTSWDFMGVKEGKNTKRNL------AIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKW 183
Query: 181 KGVCMEAPDFKKSNCNRKLVGARYYNAIEPNGNDSRVGMPKGTPRDLLGHGTHTSSIAAG 240
KGVC +F CN KL+GAR Y + +GT RD GHGTHT+S AAG
Sbjct: 184 KGVCSGGKNF---TCNNKLIGARDYTS-------------EGT-RDTSGHGTHTASTAAG 243
Query: 241 ARVPNASYFDLARGTARGGGTVSTRIASYKVCAGVGCSGAAILKAIDDAVKDGVDIISIS 300
V + S+F + GT R GG ++RIA+YKVC GCS A+L + DDA+ DGVD+I+IS
Sbjct: 244 NAVKDTSFFGIGNGTVR-GGVPASRIAAYKVCTDSGCSSEALLSSFDDAIADGVDLITIS 303
Query: 301 IGIGSPLFQSDYLNDPIAIGAFHAQQMGVLVVCSGGNDGPDPNTVGNIAPWIFTVAASNI 360
IG F S + +DPIAIGAFHA G+L V S GN GP P TV ++APWIFTVAAS
Sbjct: 304 IGF---QFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTVSHVAPWIFTVAASTT 363
Query: 361 DRDFQSTVVLGNGKTFQGTAINLSNLTTSKTYPLVFGKDAAAKFTPISEARNCYPGSLDR 420
+R F + VVLGNGKT G ++N ++ K YPLV+GK AA+ A C P L++
Sbjct: 364 NRGFITKVVLGNGKTLAGRSVNAFDM-KGKKYPLVYGKSAASSACDAKTAALCAPACLNK 423
Query: 421 SKVAGKIVICTSDDFSTSRTIKELVVQDAKAMGLI-LINEASKTVPLDSNIFPFTQIGNS 480
S+V GKI++C + AK++G I +I+++ + ++ P + +
Sbjct: 424 SRVKGKILVCGGPS----------GYKIAKSVGAIAIIDKSPRPDVAFTHHLPASGLKAK 483
Query: 481 EGLQILEYINSTKNPTATILRTVEVRRLKPAPMVAYFSSRGPSPLTENILKPDITAPGVS 540
+ ++ YI S +P A +L+T + + +P++A FSSRGP+ + +ILKPDITAPGV
Sbjct: 484 DFKSLVSYIESQDSPQAAVLKTETIFN-RTSPVIASFSSRGPNTIAVDILKPDITAPGVE 543
Query: 541 ILAAVVPKSDAESGPIGKKPSDYAMRSGTSMACPHVAGAAAFVKSVRHNWSSSMIKSALM 600
ILAA P + E + Y++ SGTSMACPHVAG AA+VK+ WS SMI+SA+M
Sbjct: 544 ILAAFSP--NGEPSEDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQSAIM 603
Query: 601 TTATIYDNQRKFMRNSTNNPSNPHEMGAGEISPIKALNPGLVFESTNEDYLRFLCYYGYS 660
TTA S GAG + P+ ALNPGLV+E D++ FLC Y+
Sbjct: 604 TTA-----------KGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYT 663
Query: 661 NKIIRSVSKQNFSCPKTSSEDLISSVNYPSISIGKLDRKQA--AKVIERTVTNVGAPNTT 720
+K ++ +S C K ++ L ++NYPS+S KL + + RT+TNVG PN+T
Sbjct: 664 SKTLKIISGDTVKCSK-KNKILPRNLNYPSMS-AKLSGTDSTFSVTFNRTLTNVGTPNST 723
Query: 721 YIAKVHSSEG--LLVKVIPSKIVFSENVKKVTFKVSFYGKEARSGY-NFGTITWRDSVHS 780
Y +KV + G L +KV PS + F +K +F V+ G + S + + W D H+
Sbjct: 724 YKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDGTHN 725
Query: 781 VRTVFAVNV 784
VR+ V +
Sbjct: 784 VRSPIVVYI 725
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q9LNU1 | 4.4e-181 | 46.42 | CO(2)-response secreted protease OS=Arabidopsis thaliana OX=3702 GN=CRSP PE=2 SV... | [more] |
F4HSQ2 | 2.8e-159 | 42.09 | Subtilisin-like protease SBT5.1 OS=Arabidopsis thaliana OX=3702 GN=SBT5.1 PE=3 S... | [more] |
A9JQS7 | 5.1e-145 | 39.34 | Subtilisin-like serine-protease S OS=Lotus japonicus OX=34305 GN=SbtS PE=2 SV=1 | [more] |
Q9FGU3 | 1.9e-139 | 41.01 | Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 S... | [more] |
Q8L7D2 | 5.5e-139 | 40.05 | Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1HAX7 | 0.0e+00 | 90.78 | CO(2)-response secreted protease-like OS=Cucurbita moschata OX=3662 GN=LOC111462... | [more] |
A0A6J1K3Y1 | 0.0e+00 | 90.14 | CO(2)-response secreted protease-like OS=Cucurbita maxima OX=3661 GN=LOC11149210... | [more] |
A0A5A7VC87 | 0.0e+00 | 87.89 | CO(2)-response secreted protease-like OS=Cucumis melo var. makuwa OX=1194695 GN=... | [more] |
A0A6J1BRZ1 | 0.0e+00 | 88.66 | CO(2)-response secreted protease-like isoform X2 OS=Momordica charantia OX=3673 ... | [more] |
A0A6J1BV63 | 0.0e+00 | 88.32 | CO(2)-response secreted protease-like isoform X1 OS=Momordica charantia OX=3673 ... | [more] |
Match Name | E-value | Identity | Description | |
XP_022961732.1 | 0.0e+00 | 90.78 | CO(2)-response secreted protease-like [Cucurbita moschata] | [more] |
KAG7029593.1 | 0.0e+00 | 90.78 | CO(2)-response secreted protease [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
XP_022997067.1 | 0.0e+00 | 90.14 | CO(2)-response secreted protease-like [Cucurbita maxima] | [more] |
XP_023547000.1 | 0.0e+00 | 90.41 | CO(2)-response secreted protease-like [Cucurbita pepo subsp. pepo] | [more] |
KAG6598653.1 | 0.0e+00 | 90.57 | CO(2)-response secreted protease, partial [Cucurbita argyrosperma subsp. sororia... | [more] |