Lcy01g013250 (gene) Sponge gourd (P93075) v1

Overview
NameLcy01g013250
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionGrpE protein homolog
LocationChr01: 34491597 .. 34494523 (+)
RNA-Seq ExpressionLcy01g013250
SyntenyLcy01g013250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGAGACCTAGGGCTCAACTAAAACGAAAGTACTTTCATTAACGAAATTACCAGAAACTCAGAGAAGGAAGAAGAAGAGGACTCGTAACAGGAGGAAAGCTCAGTTACTCAATTCCAATTTGCTTCATCGAGTTTGAGCTTCATCTTCTCTGTCAATGGCAGCTTTTGCTCTCTCTAATTACACCCTCTTTTCTCCTCGCCCTTCTACTTCATTCATTTCATCTTCCTCCAAAACCCTAGATTCCGCATCCCTTATTCAACAACCTCTTTGCCTTCTTCATAATCCTCGCCATCACTCTCGTCTTTGTAACCCATTATTAAGTTTCTCTCCCACCTCCCCTTCTTCTCCAGTTCGACGTTCTTTCGTATCGGGTCTTTCTGCTCAAGATTCTGTTGCCAATGTACGTTTAAAATCTCCTTTTATCTGTTGCTTTGATTGTTTACAGTTCTTGTTTTATCATTTTCAATGATATTTGTGCTTAATTTTGTTTCTCTGTCACGTCGTTAGTTTAGTCTTTTGCTTGTGGATGTTTTGCTGTATTTGAATTGCTGAATGTTTGAAATGGAAATGTAGGCCCGATAACCATTTGGTTTTTTGTTTTTGGTTTTTGAAAATTAAGCTTGTTTTCTCCTAATTTCCTAAGAATAGTTTTCATCTACATTTGCATTCCTAGCCAAATTCCAAAAACAAAAACAAGTTTTTGAAAACTACTTTTTTTAGTTTTCGAAACTTGGAGTTGGTTTTTGAAAACATGGAAAGAAGATATATAACAATACAAAGAAACTTATGGGTGAAAATAGTGTTTGTAGGCTTAATTTTCAAAAACCAAAAACCAAAAATCAAATGGTTATCAAACGGAGCCTTAGCTATCATTTTTTTCATTTGTATGGATTCTTAAATGCATTTTTGGAACTTCGAATTCCTATTTTCTTAGTGCTGGTTTGGTTTGAGATCTTTGCTGCTAGGCCAACCCTTAATGGCATTGATTAGACTACTCAATTTTGAGGTGAAAGAGTTTTTTTTAGAGAAAGAAAAGGCCGGTGGGATTTGATAAGGTGGTGGTTTAAGTCAGTTTTGACGTCAAATTTTGGCATTCCTTGAAAATTGTAGGTTTGATTGGCGGCCATTTTGGCTAAAAACTCAACCACCTGTTCTCCTGAATTATACTGTTTCTGTACTAATAAACATTTACAGAACTTCTTTATGGTGTAGTTGCTAACACGGCGGAGGATTTAACGTTTTCTTTGTTCTTGTACTTTGTAAGTGAAACAAGAGTGCAAGTATAGCTTAGTCTAATTGTTTGAAAATAATTTATCTCAAGAATTGAGAAAGTAACAGAAATAGAAAGGGTCTTTGGAAGTTCTTGGAGGATTCTCATTACTTTCTTCCCTTTATGGTATTCATGCTCTTCATTTCCTTAGTAGGCTTAGAAGTTACAGGTAACAACTCAATGGTTAAATGGATTTAGCACTCTTATCTAATTGAAATTTTTCTGCGAGATCCTTTTAGATGTTCAATTCAATTCACTGCTGGATGACAAAGTTACTTTATCTTTTTCTTTATTCACTTATTCAATGTAGTTGTTGGTTGCCTGCCCCTCCTATGAAAAAAGGTAAATAAAGAAGAGGATGAAGGAAAAGCTGTGGAAAAGGCAGGAGATCAGCTTCATGGATTGAGTTTGCAGGCCCTCATCAATATATACCGAGAAGCTTTGCTTGATGGAGATCAGAAGACTGTTTCTGAAGTTGAGGCAAGACTAAAAGTAATTGAGAAGGAGAAGGATGGGTTGTTCCAGAAAGTGTCCAAAATATCGGCAGAGATAACATCTGGAAAAGAGAAATATATAAGGTTACAAGCTGATTTTGATAATTTCAGGAAAAGATCAGAAAAAGAAGGGCATACCGTGAAGAATAATGCCCAGAAAGAGGTTATTGTGAGTCTATTACCAATGATCGACACTTTTGAGAAAGCCAGACAACAAATTGTTCCTCAAACTGACAAGGAGAAAAAGATCGATGTAAGCTACCAGGGTATATACAAGCAGTTTGTGGAGACGTTGAGGAGCTGGCGAGTCTCGGCCGTTGCCACAGTTGGAAGGCCATTTGATCCCTCAGTAAGTTAAATCTTTTGACTAAAACAGGATACCTTCATTTCAATGTACAAATTTGTCTATTTGATGCATGATCTTCTTCAAGCTTGCTAATTTTTCTTACTGTTACAAGGAGGATTAGAACAGAATAAATATTTATTTTCTCAGTGTTTATACTGTATCAGAACAGAATAAGTATTCATTTTCTCATTGTTTATACTGGATTAGAACAGGATAAATATTTATTTTCTCATTGTTTATACTTGGAATTCTTTGCAAGTAATTATTGTCAAGTGTATGACAGTATCAGTAACTGGGAACTGATAATGTCTGATTGGTAGTGAGAATGTTTTCAAATGAAGAGATGTGTTCTTTTCTAATGGATTGTCATGATGAGGGTAAAATATTTCTTTGGTTGCAGCTACATGAAGCTGTAGCTCGAGAGGAGTCGCAGGAGATCAAGGAAGGAATCATTATTCAAGAATTACGACGCGGTTTTCTGCTCGGCGAGCGACTTCTAAGACCAGCAAGAGTTAAAGTTTCAAAAGGTCCAGGTAGGAAGAATTCCCCCGCAATCGACTCCGAGAAACCGACCGAGCAACCTGCAACTGCTGCTGGAGTAGATGAACACTAATTGTGCAACGCCATTTTTTGGTTCATCTCAAAGCATCTGATTTATTGTCTTAATACAGCACTATGATGTAAATGTATCTCTTAGACTTTCTTTTTCAACAAGGTTTTTTATTCTTACCATTTTGTGGAGGGCATTGTAGAACTCACTCTCCATGTTTTACACACATGATAAAGGGCAAAATTGGGTTTAAAATTTTATTTAATC

mRNA sequence

CGAGACCTAGGGCTCAACTAAAACGAAAGTACTTTCATTAACGAAATTACCAGAAACTCAGAGAAGGAAGAAGAAGAGGACTCGTAACAGGAGGAAAGCTCAGTTACTCAATTCCAATTTGCTTCATCGAGTTTGAGCTTCATCTTCTCTGTCAATGGCAGCTTTTGCTCTCTCTAATTACACCCTCTTTTCTCCTCGCCCTTCTACTTCATTCATTTCATCTTCCTCCAAAACCCTAGATTCCGCATCCCTTATTCAACAACCTCTTTGCCTTCTTCATAATCCTCGCCATCACTCTCGTCTTTGTAACCCATTATTAAGTTTCTCTCCCACCTCCCCTTCTTCTCCAGTTCGACGTTCTTTCGTATCGGGTCTTTCTGCTCAAGATTCTGTTGCCAATGTAAATAAAGAAGAGGATGAAGGAAAAGCTGTGGAAAAGGCAGGAGATCAGCTTCATGGATTGAGTTTGCAGGCCCTCATCAATATATACCGAGAAGCTTTGCTTGATGGAGATCAGAAGACTGTTTCTGAAGTTGAGGCAAGACTAAAAGTAATTGAGAAGGAGAAGGATGGGTTGTTCCAGAAAGTGTCCAAAATATCGGCAGAGATAACATCTGGAAAAGAGAAATATATAAGGTTACAAGCTGATTTTGATAATTTCAGGAAAAGATCAGAAAAAGAAGGGCATACCGTGAAGAATAATGCCCAGAAAGAGGTTATTGTGAGTCTATTACCAATGATCGACACTTTTGAGAAAGCCAGACAACAAATTGTTCCTCAAACTGACAAGGAGAAAAAGATCGATGTAAGCTACCAGGGTATATACAAGCAGTTTGTGGAGACGTTGAGGAGCTGGCGAGTCTCGGCCGTTGCCACAGTTGGAAGGCCATTTGATCCCTCACTACATGAAGCTGTAGCTCGAGAGGAGTCGCAGGAGATCAAGGAAGGAATCATTATTCAAGAATTACGACGCGGTTTTCTGCTCGGCGAGCGACTTCTAAGACCAGCAAGAGTTAAAGTTTCAAAAGGTCCAGGTAGGAAGAATTCCCCCGCAATCGACTCCGAGAAACCGACCGAGCAACCTGCAACTGCTGCTGGAGTAGATGAACACTAATTGTGCAACGCCATTTTTTGGTTCATCTCAAAGCATCTGATTTATTGTCTTAATACAGCACTATGATGTAAATGTATCTCTTAGACTTTCTTTTTCAACAAGGTTTTTTATTCTTACCATTTTGTGGAGGGCATTGTAGAACTCACTCTCCATGTTTTACACACATGATAAAGGGCAAAATTGGGTTTAAAATTTTATTTAATC

Coding sequence (CDS)

ATGGCAGCTTTTGCTCTCTCTAATTACACCCTCTTTTCTCCTCGCCCTTCTACTTCATTCATTTCATCTTCCTCCAAAACCCTAGATTCCGCATCCCTTATTCAACAACCTCTTTGCCTTCTTCATAATCCTCGCCATCACTCTCGTCTTTGTAACCCATTATTAAGTTTCTCTCCCACCTCCCCTTCTTCTCCAGTTCGACGTTCTTTCGTATCGGGTCTTTCTGCTCAAGATTCTGTTGCCAATGTAAATAAAGAAGAGGATGAAGGAAAAGCTGTGGAAAAGGCAGGAGATCAGCTTCATGGATTGAGTTTGCAGGCCCTCATCAATATATACCGAGAAGCTTTGCTTGATGGAGATCAGAAGACTGTTTCTGAAGTTGAGGCAAGACTAAAAGTAATTGAGAAGGAGAAGGATGGGTTGTTCCAGAAAGTGTCCAAAATATCGGCAGAGATAACATCTGGAAAAGAGAAATATATAAGGTTACAAGCTGATTTTGATAATTTCAGGAAAAGATCAGAAAAAGAAGGGCATACCGTGAAGAATAATGCCCAGAAAGAGGTTATTGTGAGTCTATTACCAATGATCGACACTTTTGAGAAAGCCAGACAACAAATTGTTCCTCAAACTGACAAGGAGAAAAAGATCGATGTAAGCTACCAGGGTATATACAAGCAGTTTGTGGAGACGTTGAGGAGCTGGCGAGTCTCGGCCGTTGCCACAGTTGGAAGGCCATTTGATCCCTCACTACATGAAGCTGTAGCTCGAGAGGAGTCGCAGGAGATCAAGGAAGGAATCATTATTCAAGAATTACGACGCGGTTTTCTGCTCGGCGAGCGACTTCTAAGACCAGCAAGAGTTAAAGTTTCAAAAGGTCCAGGTAGGAAGAATTCCCCCGCAATCGACTCCGAGAAACCGACCGAGCAACCTGCAACTGCTGCTGGAGTAGATGAACACTAA

Protein sequence

MAAFALSNYTLFSPRPSTSFISSSSKTLDSASLIQQPLCLLHNPRHHSRLCNPLLSFSPTSPSSPVRRSFVSGLSAQDSVANVNKEEDEGKAVEKAGDQLHGLSLQALINIYREALLDGDQKTVSEVEARLKVIEKEKDGLFQKVSKISAEITSGKEKYIRLQADFDNFRKRSEKEGHTVKNNAQKEVIVSLLPMIDTFEKARQQIVPQTDKEKKIDVSYQGIYKQFVETLRSWRVSAVATVGRPFDPSLHEAVAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPGRKNSPAIDSEKPTEQPATAAGVDEH
Homology
BLAST of Lcy01g013250 vs. ExPASy Swiss-Prot
Match: Q7V3Q4 (Protein GrpE OS=Prochlorococcus marinus subsp. pastoris (strain CCMP1986 / NIES-2087 / MED4) OX=59919 GN=grpE PE=3 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 7.9e-36
Identity = 96/244 (39.34%), Postives = 141/244 (57.79%), Query Frame = 0

Query: 77  QDSVA-NVNKEEDEGKAVEKAGDQLHGLSLQALINIYREALLDGDQKTVSEVEARLKVIE 136
           +D+V+  VNK ED      +  +    L +    NIY E L    + T++  +ARL+ +E
Sbjct: 13  EDNVSQEVNKNEDSSVIENQTSEDKQTLEVDD-ENIYAEDL----KNTITNNDARLEQLE 72

Query: 137 KEKDGLFQKVSKISAEITSGKEKYIRLQADFDNFRKRSEKEGHTVKNNAQKEVIVSLLPM 196
           KE + L              K +Y+R+ ADFDNFRKR  ++   +K     + + ++LP+
Sbjct: 73  KEHETL--------------KSQYVRIAADFDNFRKRQSRDQDDLKVQLVSKALTAILPI 132

Query: 197 IDTFEKARQQIVPQTDKEKKIDVSYQGIYKQFVETLRSWRVSAVATVGRPFDPSLHEAVA 256
           +D FE+ARQQ+ P++++ + +  SYQG+YKQ VE L+   VS +  V + FDP LHEAV 
Sbjct: 133 VDNFERARQQLKPESEEAQTLHRSYQGLYKQLVEVLKQQGVSPMRVVAQQFDPKLHEAVL 192

Query: 257 REESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPGRKNSPA----------IDSEK 310
           RE SQE  E III+EL+RG+ L  ++LR A VKVS GPG++NS            IDSE 
Sbjct: 193 REPSQEFNEDIIIEELQRGYHLEGKVLRHALVKVSMGPGQQNSQEPEEKDKVEEDIDSEN 237

BLAST of Lcy01g013250 vs. ExPASy Swiss-Prot
Match: Q31DG8 (Protein GrpE OS=Prochlorococcus marinus (strain MIT 9312) OX=74546 GN=grpE PE=3 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 5.1e-35
Identity = 95/246 (38.62%), Postives = 141/246 (57.32%), Query Frame = 0

Query: 74  LSAQDSVANVNKEEDEGKAVEKAGDQLHGLSLQALINIYREALLDGDQKTVSEVEARLKV 133
           +   D +   N  ED   A EK  +    LS Q    I  E L    + T+S  +ARL+ 
Sbjct: 11  IKENDVLNQDNAPEDNSSAAEKTIEN-DELSPQKTEEINTEEL----KNTISNNDARLEQ 70

Query: 134 IEKEKDGLFQKVSKISAEITSGKEKYIRLQADFDNFRKRSEKEGHTVKNNAQKEVIVSLL 193
           +EKE + L              K +Y+R+ ADFDNFRKR  ++   +K     + + ++L
Sbjct: 71  LEKEHETL--------------KNQYVRISADFDNFRKRQSRDQDDLKVQLVSKTLTAIL 130

Query: 194 PMIDTFEKARQQIVPQTDKEKKIDVSYQGIYKQFVETLRSWRVSAVATVGRPFDPSLHEA 253
           P++D FE+ARQQ+ P++++ + +  SYQG+YKQ VE L+   VS +  VG+ FDP+LHEA
Sbjct: 131 PIVDNFERARQQLKPESEEAQALHRSYQGLYKQLVEVLKQQGVSPMRVVGQQFDPNLHEA 190

Query: 254 VAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPGRKNSPA----------IDS 310
           V RE S+E KE +I++EL+RG+ L  ++LR A VKVS G G++NS            IDS
Sbjct: 191 VLREPSEEFKEDLIVEELQRGYHLEGKVLRHALVKVSMGHGQQNSQEEVEKDTVEEDIDS 237

BLAST of Lcy01g013250 vs. ExPASy Swiss-Prot
Match: A8G203 (Protein GrpE OS=Prochlorococcus marinus (strain MIT 9215) OX=93060 GN=grpE PE=3 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 6.7e-35
Identity = 79/178 (44.38%), Postives = 117/178 (65.73%), Query Frame = 0

Query: 121 QKTVSEVEARLKVIEKEKDGLFQKVSKISAEITSGKEKYIRLQADFDNFRKRSEKEGHTV 180
           + T+S  +ARLK +EKE + L              K +Y+R+ ADFDNFRKR  ++   +
Sbjct: 53  KNTISNNDARLKQLEKEHETL--------------KNQYVRISADFDNFRKRQSRDQDDL 112

Query: 181 KNNAQKEVIVSLLPMIDTFEKARQQIVPQTDKEKKIDVSYQGIYKQFVETLRSWRVSAVA 240
           K     + + ++LP++D FE+ARQQ+ P++++ + +  SYQG+YKQ VE L+   VS + 
Sbjct: 113 KIQIVSKTLTAILPIVDNFERARQQLQPESEEAQALHRSYQGLYKQLVEVLKQQGVSPMR 172

Query: 241 TVGRPFDPSLHEAVAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPGRKNS 299
            VG+ FDP+LHEAV RE S+E  E  II+EL+RG+ L  ++LR A VKVS GPG++NS
Sbjct: 173 VVGQQFDPNLHEAVLREPSEESDEDFIIEELQRGYHLEGKVLRHALVKVSMGPGKQNS 216

BLAST of Lcy01g013250 vs. ExPASy Swiss-Prot
Match: A2BNE2 (Protein GrpE OS=Prochlorococcus marinus (strain AS9601) OX=146891 GN=grpE PE=3 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 2.5e-34
Identity = 82/199 (41.21%), Postives = 125/199 (62.81%), Query Frame = 0

Query: 121 QKTVSEVEARLKVIEKEKDGLFQKVSKISAEITSGKEKYIRLQADFDNFRKRSEKEGHTV 180
           + ++S  +ARL+ +EKE + L              K +Y+R+ ADFDNFRKR  ++   +
Sbjct: 53  KNSISNNDARLEQLEKEHETL--------------KNQYVRISADFDNFRKRQSRDQDDL 112

Query: 181 KNNAQKEVIVSLLPMIDTFEKARQQIVPQTDKEKKIDVSYQGIYKQFVETLRSWRVSAVA 240
           K     + + ++LP++D FE+ARQQ+ P++++ + +  SYQG+YKQ VE L+   VS + 
Sbjct: 113 KIQLVSKTLTAILPIVDNFERARQQLKPESEEAQSLHRSYQGLYKQLVEVLKQQGVSPMR 172

Query: 241 TVGRPFDPSLHEAVAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPGRKNSPA 300
            VG+ FDPSLHEAV RE S++ +E  II+EL+RG+ L  ++LR A VKVS GPG++NS  
Sbjct: 173 VVGQQFDPSLHEAVLREPSEKFEEDFIIEELQRGYHLEGKVLRHALVKVSMGPGKQNSQE 232

Query: 301 ----------IDSEKPTEQ 310
                     IDSE+ T +
Sbjct: 233 EVEKDKVEGDIDSEENTSE 237

BLAST of Lcy01g013250 vs. ExPASy Swiss-Prot
Match: A3PA63 (Protein GrpE OS=Prochlorococcus marinus (strain MIT 9301) OX=167546 GN=grpE PE=3 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 1.3e-33
Identity = 85/215 (39.53%), Postives = 131/215 (60.93%), Query Frame = 0

Query: 84  NKEEDEGKAVEKAGDQLHGLSLQALINIYREALLDGDQKTVSEVEARLKVIEKEKDGLFQ 143
           N  ED+  +++ +  +   LS Q    I  E L    + T+S  +ARL+ +EKE + L  
Sbjct: 21  NAPEDK-SSMQNSTTENDELSSQKTEEINTEEL----KNTISNNDARLEQLEKEHETL-- 80

Query: 144 KVSKISAEITSGKEKYIRLQADFDNFRKRSEKEGHTVKNNAQKEVIVSLLPMIDTFEKAR 203
                       K +Y+R+ ADFDNFRKR  ++   +K     + + ++LP++D FE+AR
Sbjct: 81  ------------KNQYVRISADFDNFRKRQSRDQDDLKIQLVSKTLTAILPIVDNFERAR 140

Query: 204 QQIVPQTDKEKKIDVSYQGIYKQFVETLRSWRVSAVATVGRPFDPSLHEAVAREESQEIK 263
           QQ+ P++++ + +  SYQG+YKQ VE L+   VS +  VG+ FDP+LHEAV RE S+E +
Sbjct: 141 QQLKPESEEAQALHRSYQGLYKQLVEVLKQQGVSPMRVVGQQFDPNLHEAVLREPSEEFE 200

Query: 264 EGIIIQELRRGFLLGERLLRPARVKVSKGPGRKNS 299
           E  II+EL+RG+ L  ++LR A  KVS GPG++ S
Sbjct: 201 EDFIIEELQRGYHLEGKVLRHALAKVSMGPGKQKS 216

BLAST of Lcy01g013250 vs. ExPASy TrEMBL
Match: A0A6J1BPS6 (GrpE protein homolog OS=Momordica charantia OX=3673 GN=LOC111004544 PE=3 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 7.0e-144
Identity = 279/321 (86.92%), Postives = 297/321 (92.52%), Query Frame = 0

Query: 1   MAAFALSNYTLFSPRPST--SFISSSSKTLDSASLIQQPLCLLHNPRHHSRLCNPLLSFS 60
           MAAFALSNY+LFSPRPST  SFISSSS+TL+S S IQQPLCLLHNPR   RLC PLLSFS
Sbjct: 1   MAAFALSNYSLFSPRPSTSFSFISSSSETLESTSPIQQPLCLLHNPRRSPRLCKPLLSFS 60

Query: 61  PTSPSSPVRRSFVSGLSAQDSVANVNKEEDEGKAVEKAGDQLHGLSLQALINIYREALLD 120
           P S SS VRRSF+S LSAQDSVANV+KEED+GKAVEKAGDQLH  SLQ LIN+YREA LD
Sbjct: 61  P-SCSSAVRRSFISCLSAQDSVANVHKEEDDGKAVEKAGDQLHESSLQTLINVYREAFLD 120

Query: 121 GDQKTVSEVEARLKVIEKEKDGLFQKVSKISAEITSGKEKYIRLQADFDNFRKRSEKEGH 180
           GDQKTVSEVEARLK+IE+EK+GLFQKVS ISAEITSGKEKYIRLQADFDNFRKRSEKE H
Sbjct: 121 GDQKTVSEVEARLKIIEREKNGLFQKVSSISAEITSGKEKYIRLQADFDNFRKRSEKERH 180

Query: 181 TVKNNAQKEVIVSLLPMIDTFEKARQQIVPQTDKEKKIDVSYQGIYKQFVETLRSWRVSA 240
           TVKNNAQK+VI SLLPMID FEKARQQI+PQTDKEKKIDVSYQGIYKQFVETLRSW+VS+
Sbjct: 181 TVKNNAQKQVIESLLPMIDNFEKARQQIIPQTDKEKKIDVSYQGIYKQFVETLRSWQVSS 240

Query: 241 VATVGRPFDPSLHEAVAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPGRKNS 300
           VATVGRPFDPSLHEA+AREES EIKEGIIIQEL+RGFLLGERLLRPARVKVS+GPGRKNS
Sbjct: 241 VATVGRPFDPSLHEAIAREESLEIKEGIIIQELQRGFLLGERLLRPARVKVSRGPGRKNS 300

Query: 301 PAIDSEKPTEQPATAAGVDEH 320
           P I+SEKPTEQ ATAA VDEH
Sbjct: 301 PTINSEKPTEQRATAAQVDEH 320

BLAST of Lcy01g013250 vs. ExPASy TrEMBL
Match: A0A6J1GI26 (GrpE protein homolog OS=Cucurbita moschata OX=3662 GN=LOC111454347 PE=3 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 4.1e-136
Identity = 264/320 (82.50%), Postives = 281/320 (87.81%), Query Frame = 0

Query: 1   MAAFALSNYTLFSPRPSTSFISSSSKTLDSASLIQQPLCLLHNPRHHSRLCNPLLSFSPT 60
           MAA ALSNYTLFSPRPST     SSK ++S SL+   LC LHNPRH SR C PLLSFSP 
Sbjct: 1   MAALALSNYTLFSPRPST-----SSKAIESTSLVTPSLCFLHNPRHRSRHCYPLLSFSPA 60

Query: 61  SPSSPVRRSFVSGLSAQDSVANVNKEEDEGKAVEKAGDQLHGLSLQALINIYREALLDGD 120
           S SSPVRRSF+   SAQDSV NVNKEED+G+   KAGD+LHGLSLQALINIYREA LDGD
Sbjct: 61  SHSSPVRRSFLPCFSAQDSVPNVNKEEDDGEDGNKAGDELHGLSLQALINIYREAFLDGD 120

Query: 121 QKTVSEVEARLKVIEKEKDGLFQKVSKISAEITSGKEKYIRLQADFDNFRKRSEKEGHTV 180
           QKT+S+VEA+LK+ E+EKDGLFQKVS  SAEITSGKE YIRLQADFDNFRKRSEKE  TV
Sbjct: 121 QKTISKVEAKLKIFEREKDGLFQKVSNTSAEITSGKENYIRLQADFDNFRKRSEKERLTV 180

Query: 181 KNNAQKEVIVSLLPMIDTFEKARQQIVPQTDKEKKIDVSYQGIYKQFVETLRSWRVSAVA 240
           KNNAQKEVI +LLPMID+FEKARQQIVPQTDKEKKIDVSYQGIYKQFVE LRSWR+SAVA
Sbjct: 181 KNNAQKEVIENLLPMIDSFEKARQQIVPQTDKEKKIDVSYQGIYKQFVEVLRSWRISAVA 240

Query: 241 TVGRPFDPSLHEAVAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPGRKNSPA 300
            VGRPFDPSLHEAVAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPG+KNSP 
Sbjct: 241 AVGRPFDPSLHEAVAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPGKKNSPT 300

Query: 301 IDSEKPTEQPATA-AGVDEH 320
            +SEKPTE PATA A VDEH
Sbjct: 301 TNSEKPTEHPATATARVDEH 315

BLAST of Lcy01g013250 vs. ExPASy TrEMBL
Match: A0A6J1GHY1 (GrpE protein homolog OS=Cucurbita moschata OX=3662 GN=LOC111454347 PE=3 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 1.0e-134
Identity = 264/321 (82.24%), Postives = 281/321 (87.54%), Query Frame = 0

Query: 1   MAAFALSNYTLFSPRPSTSFISSSSKTLDSASLIQQPLCLLHNPRHHSRLCNPLLSFSPT 60
           MAA ALSNYTLFSPRPST     SSK ++S SL+   LC LHNPRH SR C PLLSFSP 
Sbjct: 1   MAALALSNYTLFSPRPST-----SSKAIESTSLVTPSLCFLHNPRHRSRHCYPLLSFSPA 60

Query: 61  SPSSPVRRSFVSGLSAQDSVAN-VNKEEDEGKAVEKAGDQLHGLSLQALINIYREALLDG 120
           S SSPVRRSF+   SAQDSV N VNKEED+G+   KAGD+LHGLSLQALINIYREA LDG
Sbjct: 61  SHSSPVRRSFLPCFSAQDSVPNKVNKEEDDGEDGNKAGDELHGLSLQALINIYREAFLDG 120

Query: 121 DQKTVSEVEARLKVIEKEKDGLFQKVSKISAEITSGKEKYIRLQADFDNFRKRSEKEGHT 180
           DQKT+S+VEA+LK+ E+EKDGLFQKVS  SAEITSGKE YIRLQADFDNFRKRSEKE  T
Sbjct: 121 DQKTISKVEAKLKIFEREKDGLFQKVSNTSAEITSGKENYIRLQADFDNFRKRSEKERLT 180

Query: 181 VKNNAQKEVIVSLLPMIDTFEKARQQIVPQTDKEKKIDVSYQGIYKQFVETLRSWRVSAV 240
           VKNNAQKEVI +LLPMID+FEKARQQIVPQTDKEKKIDVSYQGIYKQFVE LRSWR+SAV
Sbjct: 181 VKNNAQKEVIENLLPMIDSFEKARQQIVPQTDKEKKIDVSYQGIYKQFVEVLRSWRISAV 240

Query: 241 ATVGRPFDPSLHEAVAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPGRKNSP 300
           A VGRPFDPSLHEAVAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPG+KNSP
Sbjct: 241 AAVGRPFDPSLHEAVAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPGKKNSP 300

Query: 301 AIDSEKPTEQPATA-AGVDEH 320
             +SEKPTE PATA A VDEH
Sbjct: 301 TTNSEKPTEHPATATARVDEH 316

BLAST of Lcy01g013250 vs. ExPASy TrEMBL
Match: A0A5A7VFZ8 (GrpE protein homolog OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G001230 PE=3 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 5.0e-134
Identity = 260/319 (81.50%), Postives = 279/319 (87.46%), Query Frame = 0

Query: 1   MAAFALSNYTLFSPRPSTSFISSSSKTLDSASLIQQPLCLLHNPRHHSRLCNPLLSFSPT 60
           MAAFALSNYTLFSPRPS+ F+SSSSKTLDS S   QPL LLHNPRH SRLC+PL +FSPT
Sbjct: 1   MAAFALSNYTLFSPRPSSPFLSSSSKTLDSTSPFHQPLPLLHNPRHRSRLCDPLFNFSPT 60

Query: 61  SPSSPVRRSFVSGLSAQDSVANVNKEEDEGKAVEKAGDQLHGLSLQALINIYREALLDGD 120
           S SSP+ RSF   LSA  SVANVN EED+GKAVEK G +  G SLQ LI +YREA LDGD
Sbjct: 61  SSSSPLTRSFAPCLSAHSSVANVNNEEDDGKAVEKDGYKFDGSSLQTLIEVYREAFLDGD 120

Query: 121 QKTVSEVEARLKVIEKEKDGLFQKVSKISAEITSGKEKYIRLQADFDNFRKRSEKEGHTV 180
           QKTVSEVEAR+K+I +EKD L +K+S IS E+TSGKEKYIRLQADFDNFRKRSEKE H V
Sbjct: 121 QKTVSEVEARIKIIGREKDELSRKLSNISTEMTSGKEKYIRLQADFDNFRKRSEKEQHNV 180

Query: 181 KNNAQKEVIVSLLPMIDTFEKARQQIVPQTDKEKKIDVSYQGIYKQFVETLRSWRVSAVA 240
           KNNAQKEVI SLLPMID FEKARQQIVPQTDKEKKID+SYQGIYKQFVETLRSWRVSAVA
Sbjct: 181 KNNAQKEVIESLLPMIDHFEKARQQIVPQTDKEKKIDISYQGIYKQFVETLRSWRVSAVA 240

Query: 241 TVGRPFDPSLHEAVAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPGRKNSPA 300
           TVGRPFDPSLHEAVAREESQEIKEGI+IQELRRGFLLGERLLRPARVKVSKGPGRK+S  
Sbjct: 241 TVGRPFDPSLHEAVAREESQEIKEGIVIQELRRGFLLGERLLRPARVKVSKGPGRKSSRT 300

Query: 301 IDSEKPTEQPATAAGVDEH 320
           ID +   +QPA AAGVDEH
Sbjct: 301 IDGK---QQPAAAAGVDEH 316

BLAST of Lcy01g013250 vs. ExPASy TrEMBL
Match: A0A1S3BDD3 (GrpE protein homolog OS=Cucumis melo OX=3656 GN=LOC103488418 PE=3 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 1.1e-133
Identity = 260/319 (81.50%), Postives = 278/319 (87.15%), Query Frame = 0

Query: 1   MAAFALSNYTLFSPRPSTSFISSSSKTLDSASLIQQPLCLLHNPRHHSRLCNPLLSFSPT 60
           MAAFALSNYTLFSPRPS+ F+SSSSKTLDS S   QPL LLHNPRH SRLC+PL SFSPT
Sbjct: 1   MAAFALSNYTLFSPRPSSPFLSSSSKTLDSTSPFHQPLPLLHNPRHRSRLCDPLFSFSPT 60

Query: 61  SPSSPVRRSFVSGLSAQDSVANVNKEEDEGKAVEKAGDQLHGLSLQALINIYREALLDGD 120
           S SSP+ RSF   LSA  SVANVN EED+GKAVEK G +  G SLQ LI +YREA LDGD
Sbjct: 61  SSSSPLTRSFAPCLSAHSSVANVNNEEDDGKAVEKDGYKFDGSSLQTLIEVYREAFLDGD 120

Query: 121 QKTVSEVEARLKVIEKEKDGLFQKVSKISAEITSGKEKYIRLQADFDNFRKRSEKEGHTV 180
           QKTVSEVEAR+K+I +EKD L +K+S I  E+TSGKEKYIRLQADFDNFRKRSEKE H V
Sbjct: 121 QKTVSEVEARIKIIGREKDELSRKLSNILTEMTSGKEKYIRLQADFDNFRKRSEKEQHNV 180

Query: 181 KNNAQKEVIVSLLPMIDTFEKARQQIVPQTDKEKKIDVSYQGIYKQFVETLRSWRVSAVA 240
           KNNAQKEVI SLLPMID FEKARQQIVPQTDKEKKID+SYQGIYKQFVETLRSWRVSAVA
Sbjct: 181 KNNAQKEVIESLLPMIDHFEKARQQIVPQTDKEKKIDISYQGIYKQFVETLRSWRVSAVA 240

Query: 241 TVGRPFDPSLHEAVAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPGRKNSPA 300
           TVGRPFDPSLHEAVAREESQEIKEGI+IQELRRGFLLGERLLRPARVKVSKGPGRK+S  
Sbjct: 241 TVGRPFDPSLHEAVAREESQEIKEGIVIQELRRGFLLGERLLRPARVKVSKGPGRKSSRT 300

Query: 301 IDSEKPTEQPATAAGVDEH 320
           ID +   +QPA AAGVDEH
Sbjct: 301 IDGK---QQPAAAAGVDEH 316

BLAST of Lcy01g013250 vs. NCBI nr
Match: XP_022131274.1 (uncharacterized protein LOC111004544 [Momordica charantia])

HSP 1 Score: 520.0 bits (1338), Expect = 1.4e-143
Identity = 279/321 (86.92%), Postives = 297/321 (92.52%), Query Frame = 0

Query: 1   MAAFALSNYTLFSPRPST--SFISSSSKTLDSASLIQQPLCLLHNPRHHSRLCNPLLSFS 60
           MAAFALSNY+LFSPRPST  SFISSSS+TL+S S IQQPLCLLHNPR   RLC PLLSFS
Sbjct: 1   MAAFALSNYSLFSPRPSTSFSFISSSSETLESTSPIQQPLCLLHNPRRSPRLCKPLLSFS 60

Query: 61  PTSPSSPVRRSFVSGLSAQDSVANVNKEEDEGKAVEKAGDQLHGLSLQALINIYREALLD 120
           P S SS VRRSF+S LSAQDSVANV+KEED+GKAVEKAGDQLH  SLQ LIN+YREA LD
Sbjct: 61  P-SCSSAVRRSFISCLSAQDSVANVHKEEDDGKAVEKAGDQLHESSLQTLINVYREAFLD 120

Query: 121 GDQKTVSEVEARLKVIEKEKDGLFQKVSKISAEITSGKEKYIRLQADFDNFRKRSEKEGH 180
           GDQKTVSEVEARLK+IE+EK+GLFQKVS ISAEITSGKEKYIRLQADFDNFRKRSEKE H
Sbjct: 121 GDQKTVSEVEARLKIIEREKNGLFQKVSSISAEITSGKEKYIRLQADFDNFRKRSEKERH 180

Query: 181 TVKNNAQKEVIVSLLPMIDTFEKARQQIVPQTDKEKKIDVSYQGIYKQFVETLRSWRVSA 240
           TVKNNAQK+VI SLLPMID FEKARQQI+PQTDKEKKIDVSYQGIYKQFVETLRSW+VS+
Sbjct: 181 TVKNNAQKQVIESLLPMIDNFEKARQQIIPQTDKEKKIDVSYQGIYKQFVETLRSWQVSS 240

Query: 241 VATVGRPFDPSLHEAVAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPGRKNS 300
           VATVGRPFDPSLHEA+AREES EIKEGIIIQEL+RGFLLGERLLRPARVKVS+GPGRKNS
Sbjct: 241 VATVGRPFDPSLHEAIAREESLEIKEGIIIQELQRGFLLGERLLRPARVKVSRGPGRKNS 300

Query: 301 PAIDSEKPTEQPATAAGVDEH 320
           P I+SEKPTEQ ATAA VDEH
Sbjct: 301 PTINSEKPTEQRATAAQVDEH 320

BLAST of Lcy01g013250 vs. NCBI nr
Match: XP_038884130.1 (protein GrpE [Benincasa hispida])

HSP 1 Score: 507.3 bits (1305), Expect = 9.7e-140
Identity = 268/319 (84.01%), Postives = 287/319 (89.97%), Query Frame = 0

Query: 1   MAAFALSNYTLFSPRPSTSFISSSSKTLDSASLIQQPLCLLHNPRHHSRLCNPLLSFSPT 60
           MAAFALSNYTL SPRPST  +SSSSKTL+S SLI QPLCL HNP H SRLCN LLSFSPT
Sbjct: 1   MAAFALSNYTLISPRPSTPLVSSSSKTLESTSLIHQPLCLRHNPPHRSRLCNSLLSFSPT 60

Query: 61  SPSSPVRRSFVSGLSAQDSVANVNKEEDEGKAVEKAGDQLHGLSLQALINIYREALLDGD 120
           +PSSPV RSFVS LSA DSVANVN EED+GKA+EK G Q    SLQ LI++YREA LDGD
Sbjct: 61  TPSSPVTRSFVSCLSAHDSVANVNNEEDDGKAMEKDGYQFDRYSLQTLIDVYREAFLDGD 120

Query: 121 QKTVSEVEARLKVIEKEKDGLFQKVSKISAEITSGKEKYIRLQADFDNFRKRSEKEGHTV 180
           QKTVSEVEA++K+I +EKDGLFQKVS +SAEITSGKEKYIRLQADFDNFRKRSEKE HTV
Sbjct: 121 QKTVSEVEAKIKIIGREKDGLFQKVSNVSAEITSGKEKYIRLQADFDNFRKRSEKEQHTV 180

Query: 181 KNNAQKEVIVSLLPMIDTFEKARQQIVPQTDKEKKIDVSYQGIYKQFVETLRSWRVSAVA 240
           KNNAQKEVI SLLP+ID FEKARQQIVPQTDKEKKID+SYQGIYKQFVETLRSWRVSAVA
Sbjct: 181 KNNAQKEVIESLLPIIDNFEKARQQIVPQTDKEKKIDISYQGIYKQFVETLRSWRVSAVA 240

Query: 241 TVGRPFDPSLHEAVAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPGRKNSPA 300
           TVGRPFDPSLHEAVAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPGR+NSP 
Sbjct: 241 TVGRPFDPSLHEAVAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPGRRNSPT 300

Query: 301 IDSEKPTEQPATAAGVDEH 320
           I++    ++PA AAGVDEH
Sbjct: 301 INA---GQKPAAAAGVDEH 316

BLAST of Lcy01g013250 vs. NCBI nr
Match: XP_022951572.1 (uncharacterized protein LOC111454347 isoform X2 [Cucurbita moschata])

HSP 1 Score: 494.2 bits (1271), Expect = 8.5e-136
Identity = 264/320 (82.50%), Postives = 281/320 (87.81%), Query Frame = 0

Query: 1   MAAFALSNYTLFSPRPSTSFISSSSKTLDSASLIQQPLCLLHNPRHHSRLCNPLLSFSPT 60
           MAA ALSNYTLFSPRPST     SSK ++S SL+   LC LHNPRH SR C PLLSFSP 
Sbjct: 1   MAALALSNYTLFSPRPST-----SSKAIESTSLVTPSLCFLHNPRHRSRHCYPLLSFSPA 60

Query: 61  SPSSPVRRSFVSGLSAQDSVANVNKEEDEGKAVEKAGDQLHGLSLQALINIYREALLDGD 120
           S SSPVRRSF+   SAQDSV NVNKEED+G+   KAGD+LHGLSLQALINIYREA LDGD
Sbjct: 61  SHSSPVRRSFLPCFSAQDSVPNVNKEEDDGEDGNKAGDELHGLSLQALINIYREAFLDGD 120

Query: 121 QKTVSEVEARLKVIEKEKDGLFQKVSKISAEITSGKEKYIRLQADFDNFRKRSEKEGHTV 180
           QKT+S+VEA+LK+ E+EKDGLFQKVS  SAEITSGKE YIRLQADFDNFRKRSEKE  TV
Sbjct: 121 QKTISKVEAKLKIFEREKDGLFQKVSNTSAEITSGKENYIRLQADFDNFRKRSEKERLTV 180

Query: 181 KNNAQKEVIVSLLPMIDTFEKARQQIVPQTDKEKKIDVSYQGIYKQFVETLRSWRVSAVA 240
           KNNAQKEVI +LLPMID+FEKARQQIVPQTDKEKKIDVSYQGIYKQFVE LRSWR+SAVA
Sbjct: 181 KNNAQKEVIENLLPMIDSFEKARQQIVPQTDKEKKIDVSYQGIYKQFVEVLRSWRISAVA 240

Query: 241 TVGRPFDPSLHEAVAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPGRKNSPA 300
            VGRPFDPSLHEAVAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPG+KNSP 
Sbjct: 241 AVGRPFDPSLHEAVAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPGKKNSPT 300

Query: 301 IDSEKPTEQPATA-AGVDEH 320
            +SEKPTE PATA A VDEH
Sbjct: 301 TNSEKPTEHPATATARVDEH 315

BLAST of Lcy01g013250 vs. NCBI nr
Match: KAG6585280.1 (hypothetical protein SDJN03_18013, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 491.1 bits (1263), Expect = 7.2e-135
Identity = 262/320 (81.88%), Postives = 280/320 (87.50%), Query Frame = 0

Query: 1   MAAFALSNYTLFSPRPSTSFISSSSKTLDSASLIQQPLCLLHNPRHHSRLCNPLLSFSPT 60
           MAA ALSNYTLFSPRPST     SSK ++S SL+   LC LHNPRH SR C PLLSFSP 
Sbjct: 1   MAALALSNYTLFSPRPST-----SSKAIESTSLVTPSLCFLHNPRHRSRHCYPLLSFSPA 60

Query: 61  SPSSPVRRSFVSGLSAQDSVANVNKEEDEGKAVEKAGDQLHGLSLQALINIYREALLDGD 120
           S SS VRRSF+   SAQDSV NVNKEED+G+   KAGD+LHGLSLQALIN+YREA LDGD
Sbjct: 61  SQSSTVRRSFLPCFSAQDSVPNVNKEEDDGEDGSKAGDELHGLSLQALINVYREAFLDGD 120

Query: 121 QKTVSEVEARLKVIEKEKDGLFQKVSKISAEITSGKEKYIRLQADFDNFRKRSEKEGHTV 180
           QKT+S+VEA+LK+ E+EKDGLFQKVS  SAEITSGKE YIRLQADFDNFRKRSEKE  TV
Sbjct: 121 QKTISKVEAKLKIFEREKDGLFQKVSNTSAEITSGKENYIRLQADFDNFRKRSEKERLTV 180

Query: 181 KNNAQKEVIVSLLPMIDTFEKARQQIVPQTDKEKKIDVSYQGIYKQFVETLRSWRVSAVA 240
           KNNAQKEVI +LLPMID+FEKARQQIVPQTDKEKKIDVSYQGIYKQFVE LRSWR+SAVA
Sbjct: 181 KNNAQKEVIENLLPMIDSFEKARQQIVPQTDKEKKIDVSYQGIYKQFVEVLRSWRISAVA 240

Query: 241 TVGRPFDPSLHEAVAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPGRKNSPA 300
            VGRPFDPSLHEAVAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPG+KNSP 
Sbjct: 241 AVGRPFDPSLHEAVAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPGKKNSPT 300

Query: 301 IDSEKPTEQPATA-AGVDEH 320
            +SEKPTE PATA A VDEH
Sbjct: 301 TNSEKPTEHPATATARVDEH 315

BLAST of Lcy01g013250 vs. NCBI nr
Match: KAG7020199.1 (grpE [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 490.7 bits (1262), Expect = 9.4e-135
Identity = 262/320 (81.88%), Postives = 280/320 (87.50%), Query Frame = 0

Query: 1   MAAFALSNYTLFSPRPSTSFISSSSKTLDSASLIQQPLCLLHNPRHHSRLCNPLLSFSPT 60
           MAA ALSNYTLFSPRPST     SSK ++S SL+   LC LHNPRH SR C PLLSFSP 
Sbjct: 1   MAALALSNYTLFSPRPST-----SSKAIESTSLVTPSLCFLHNPRHRSRHCYPLLSFSPA 60

Query: 61  SPSSPVRRSFVSGLSAQDSVANVNKEEDEGKAVEKAGDQLHGLSLQALINIYREALLDGD 120
           S SS VRRSF+   SAQDSV NVNKEED+G+   KAGD+LHGLSLQALIN+YREA LDGD
Sbjct: 61  SHSSTVRRSFLPCFSAQDSVPNVNKEEDDGEDGSKAGDELHGLSLQALINVYREAFLDGD 120

Query: 121 QKTVSEVEARLKVIEKEKDGLFQKVSKISAEITSGKEKYIRLQADFDNFRKRSEKEGHTV 180
           QKT+S+VEA+LK+ E+EKDGLFQKVS  SAEITSGKE YIRLQADFDNFRKRSEKE  TV
Sbjct: 121 QKTISKVEAKLKIFEREKDGLFQKVSNTSAEITSGKENYIRLQADFDNFRKRSEKERLTV 180

Query: 181 KNNAQKEVIVSLLPMIDTFEKARQQIVPQTDKEKKIDVSYQGIYKQFVETLRSWRVSAVA 240
           KNNAQKEVI +LLPMID+FEKARQQIVPQTDKEKKIDVSYQGIYKQFVE LRSWR+SAVA
Sbjct: 181 KNNAQKEVIENLLPMIDSFEKARQQIVPQTDKEKKIDVSYQGIYKQFVEVLRSWRISAVA 240

Query: 241 TVGRPFDPSLHEAVAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPGRKNSPA 300
            VGRPFDPSLHEAVAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPG+KNSP 
Sbjct: 241 AVGRPFDPSLHEAVAREESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPGKKNSPT 300

Query: 301 IDSEKPTEQPATA-AGVDEH 320
            +SEKPTE PATA A VDEH
Sbjct: 301 TNSEKPTEHPATATARVDEH 315

BLAST of Lcy01g013250 vs. TAIR 10
Match: AT1G36390.1 (Co-chaperone GrpE family protein )

HSP 1 Score: 218.0 bits (554), Expect = 1.1e-56
Identity = 127/270 (47.04%), Postives = 180/270 (66.67%), Query Frame = 0

Query: 52  NPLLSFSPTSPSS---PVRRSF--------VSGLSAQDS------VANVNKEEDEGKAVE 111
           +P  SFS  S SS   P+R +         +S L ++ S       A+     +E  + +
Sbjct: 12  SPCFSFSSPSSSSKTLPLRNNLHFHGRPSPISPLISKPSRKFPIFSAHQTNNSEEANSKQ 71

Query: 112 KAGDQLHGLSLQALINIYREALLDGDQKTVSEVEARLKVIEKEKDGLFQKVSKISAEITS 171
           +A        ++ LI  Y++ALL+GD+ +V+E+E     IEKEK+ + QKV  +S +I S
Sbjct: 72  QA-------DVKTLIRSYKQALLNGDETSVTEIETMFCKIEKEKNKMDQKVLSLSMKIAS 131

Query: 172 GKEKYIRLQADFDNFRKRSEKEGHTVKNNAQKEVIVSLLPMIDTFEKARQQIVPQTDKEK 231
            KE  IRLQADFDN RK+ +K+  + ++NA+ +++ SLLP+ID+FEKA+ Q+   TDKEK
Sbjct: 132 EKEMKIRLQADFDNTRKKLDKDRLSTESNAKVQILKSLLPIIDSFEKAKLQVRVDTDKEK 191

Query: 232 KIDVSYQGIYKQFVETLRSWRVSAVATVGRPFDPSLHEAVAREESQEIKEGIIIQELRRG 291
           KID SYQGIY+QFVE LR  RVS +ATVG+PFDP LHEA++REES+ +K GII +EL +G
Sbjct: 192 KIDTSYQGIYRQFVEVLRYLRVSVIATVGKPFDPLLHEAISREESEAVKAGIITEELNKG 251

Query: 292 FLLGERLLRPARVKVSKGPGRKNSPAIDSE 305
           F+LG+R+LRPA+VKVS GP  K +P+   E
Sbjct: 252 FVLGDRVLRPAKVKVSLGPVNKKTPSAAEE 274

BLAST of Lcy01g013250 vs. TAIR 10
Match: AT1G36390.2 (Co-chaperone GrpE family protein )

HSP 1 Score: 218.0 bits (554), Expect = 1.1e-56
Identity = 127/270 (47.04%), Postives = 180/270 (66.67%), Query Frame = 0

Query: 52  NPLLSFSPTSPSS---PVRRSF--------VSGLSAQDS------VANVNKEEDEGKAVE 111
           +P  SFS  S SS   P+R +         +S L ++ S       A+     +E  + +
Sbjct: 12  SPCFSFSSPSSSSKTLPLRNNLHFHGRPSPISPLISKPSRKFPIFSAHQTNNSEEANSKQ 71

Query: 112 KAGDQLHGLSLQALINIYREALLDGDQKTVSEVEARLKVIEKEKDGLFQKVSKISAEITS 171
           +A        ++ LI  Y++ALL+GD+ +V+E+E     IEKEK+ + QKV  +S +I S
Sbjct: 72  QA-------DVKTLIRSYKQALLNGDETSVTEIETMFCKIEKEKNKMDQKVLSLSMKIAS 131

Query: 172 GKEKYIRLQADFDNFRKRSEKEGHTVKNNAQKEVIVSLLPMIDTFEKARQQIVPQTDKEK 231
            KE  IRLQADFDN RK+ +K+  + ++NA+ +++ SLLP+ID+FEKA+ Q+   TDKEK
Sbjct: 132 EKEMKIRLQADFDNTRKKLDKDRLSTESNAKVQILKSLLPIIDSFEKAKLQVRVDTDKEK 191

Query: 232 KIDVSYQGIYKQFVETLRSWRVSAVATVGRPFDPSLHEAVAREESQEIKEGIIIQELRRG 291
           KID SYQGIY+QFVE LR  RVS +ATVG+PFDP LHEA++REES+ +K GII +EL +G
Sbjct: 192 KIDTSYQGIYRQFVEVLRYLRVSVIATVGKPFDPLLHEAISREESEAVKAGIITEELNKG 251

Query: 292 FLLGERLLRPARVKVSKGPGRKNSPAIDSE 305
           F+LG+R+LRPA+VKVS GP  K +P+   E
Sbjct: 252 FVLGDRVLRPAKVKVSLGPVNKKTPSAAEE 274

BLAST of Lcy01g013250 vs. TAIR 10
Match: AT5G17710.1 (Co-chaperone GrpE family protein )

HSP 1 Score: 201.1 bits (510), Expect = 1.4e-51
Identity = 111/230 (48.26%), Postives = 156/230 (67.83%), Query Frame = 0

Query: 80  VANVNKEEDEGKAVEKAGDQLHGLSLQALINIYREALLDGDQKTVSEVEARLKVIEKEKD 139
           V N N   +EG+A E+         + AL+  Y+EAL D ++  ++E+EA LK IE EK 
Sbjct: 90  VENENASAEEGEAEEE-----EAAVITALLKSYKEALADNNEGKIAEIEASLKSIEDEKF 149

Query: 140 GLFQKVSKISAEITSGKEKYIRLQADFDNFRKRSEKEGHTVKNNAQKEVIVSLLPMIDTF 199
            L  KV+ +S E++  +++ IR+ ADFDNFRKR+E+E   + +NAQ EV+ +LL ++D F
Sbjct: 150 LLADKVASLSNELSVERDRLIRISADFDNFRKRTERERLNLVSNAQGEVVENLLAVLDNF 209

Query: 200 EKARQQIVPQTDKEKKIDVSYQGIYKQFVETLRSWRVSAVATVGRPFDPSLHEAVAREES 259
           E+A+ QI  +T+ E+K+  SYQ IYKQFVE L S  V  V TVG+ FDP LHEA+ RE+S
Sbjct: 210 ERAKSQIKVETEGEEKVTNSYQSIYKQFVEILGSLGVIHVETVGKQFDPMLHEAIMREDS 269

Query: 260 QEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPGRKNSPAIDSEKPTEQ 310
            E +EGI+++E R+GFLLGERLLRP+ VKVS GPG +     + E+ T Q
Sbjct: 270 AEYEEGIVLEEYRKGFLLGERLLRPSMVKVSAGPGPEKPLEAEGEEATAQ 314

BLAST of Lcy01g013250 vs. TAIR 10
Match: AT5G17710.2 (Co-chaperone GrpE family protein )

HSP 1 Score: 200.7 bits (509), Expect = 1.8e-51
Identity = 109/233 (46.78%), Postives = 157/233 (67.38%), Query Frame = 0

Query: 77  QDSVANVNKEEDEGKAVEKAGDQLHGLSLQALINIYREALLDGDQKTVSEVEARLKVIEK 136
           Q++   V+ E +   A E   ++     + AL+  Y+EAL D ++  ++E+EA LK IE 
Sbjct: 84  QETDGAVDVENENASAEEGEAEEEEAAVITALLKSYKEALADNNEGKIAEIEASLKSIED 143

Query: 137 EKDGLFQKVSKISAEITSGKEKYIRLQADFDNFRKRSEKEGHTVKNNAQKEVIVSLLPMI 196
           EK  L  KV+ +S E++  +++ IR+ ADFDNFRKR+E+E   + +NAQ EV+ +LL ++
Sbjct: 144 EKFLLADKVASLSNELSVERDRLIRISADFDNFRKRTERERLNLVSNAQGEVVENLLAVL 203

Query: 197 DTFEKARQQIVPQTDKEKKIDVSYQGIYKQFVETLRSWRVSAVATVGRPFDPSLHEAVAR 256
           D FE+A+ QI  +T+ E+K+  SYQ IYKQFVE L S  V  V TVG+ FDP LHEA+ R
Sbjct: 204 DNFERAKSQIKVETEGEEKVTNSYQSIYKQFVEILGSLGVIHVETVGKQFDPMLHEAIMR 263

Query: 257 EESQEIKEGIIIQELRRGFLLGERLLRPARVKVSKGPGRKNSPAIDSEKPTEQ 310
           E+S E +EGI+++E R+GFLLGERLLRP+ VKVS GPG +     + E+ T Q
Sbjct: 264 EDSAEYEEGIVLEEYRKGFLLGERLLRPSMVKVSAGPGPEKPLEAEGEEATAQ 316

BLAST of Lcy01g013250 vs. TAIR 10
Match: AT4G26780.1 (Co-chaperone GrpE family protein )

HSP 1 Score: 59.7 bits (143), Expect = 5.0e-09
Identity = 59/257 (22.96%), Postives = 111/257 (43.19%), Query Frame = 0

Query: 58  SPTSPSSPVRRSF--VSGLSAQDSVANVNK----EEDEGKAVEKAGDQLHGLSLQALINI 117
           S TSP S  +++    S  S +   A  N+     E +    + A  +  G    A  + 
Sbjct: 69  SSTSPESDEKKTHTEASKTSEEKPTAEANQPGLDSESKDSVTDSAKRKRKGAKGAASSSS 128

Query: 118 YREALLDGDQKTVSEVEARLKVIEKEKDGLFQKVSKISAEITSGKEKYIRLQADFDNFRK 177
             ++  D D+ +  ++   +K++ ++++ L +K      EI   K+K +R  A+ +N   
Sbjct: 129 ESDSESDDDELSADDL---VKLVAEKEELLSEK----EEEIKQLKDKVLRTYAEMENVMD 188

Query: 178 RSEKEGHTVKNNAQKEVIVSLLPMIDTFEKARQQIVPQTDKEKKIDVS------------ 237
           R+ ++    K  A +    SLL + D   +A   +    +   K+D S            
Sbjct: 189 RTRRDAENTKKYAVQNFAKSLLDVADNLGRASSVV---KESFSKLDTSEDSAGAAPLLKT 248

Query: 238 -YQGI---YKQFVETLRSWRVSAVATVGRPFDPSLHEAVAREESQEIKEGIIIQELRRGF 293
             +G+    KQ  E  + + +     +  PFDP+ H AV +       EG +   L+ G+
Sbjct: 249 LLEGVEMTEKQLAEVFKKFGMEKYDPINEPFDPNRHNAVFQVPDASKPEGTVAHVLKSGY 308

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q7V3Q47.9e-3639.34Protein GrpE OS=Prochlorococcus marinus subsp. pastoris (strain CCMP1986 / NIES-... [more]
Q31DG85.1e-3538.62Protein GrpE OS=Prochlorococcus marinus (strain MIT 9312) OX=74546 GN=grpE PE=3 ... [more]
A8G2036.7e-3544.38Protein GrpE OS=Prochlorococcus marinus (strain MIT 9215) OX=93060 GN=grpE PE=3 ... [more]
A2BNE22.5e-3441.21Protein GrpE OS=Prochlorococcus marinus (strain AS9601) OX=146891 GN=grpE PE=3 S... [more]
A3PA631.3e-3339.53Protein GrpE OS=Prochlorococcus marinus (strain MIT 9301) OX=167546 GN=grpE PE=3... [more]
Match NameE-valueIdentityDescription
A0A6J1BPS67.0e-14486.92GrpE protein homolog OS=Momordica charantia OX=3673 GN=LOC111004544 PE=3 SV=1[more]
A0A6J1GI264.1e-13682.50GrpE protein homolog OS=Cucurbita moschata OX=3662 GN=LOC111454347 PE=3 SV=1[more]
A0A6J1GHY11.0e-13482.24GrpE protein homolog OS=Cucurbita moschata OX=3662 GN=LOC111454347 PE=3 SV=1[more]
A0A5A7VFZ85.0e-13481.50GrpE protein homolog OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G... [more]
A0A1S3BDD31.1e-13381.50GrpE protein homolog OS=Cucumis melo OX=3656 GN=LOC103488418 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022131274.11.4e-14386.92uncharacterized protein LOC111004544 [Momordica charantia][more]
XP_038884130.19.7e-14084.01protein GrpE [Benincasa hispida][more]
XP_022951572.18.5e-13682.50uncharacterized protein LOC111454347 isoform X2 [Cucurbita moschata][more]
KAG6585280.17.2e-13581.88hypothetical protein SDJN03_18013, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7020199.19.4e-13581.88grpE [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT1G36390.11.1e-5647.04Co-chaperone GrpE family protein [more]
AT1G36390.21.1e-5647.04Co-chaperone GrpE family protein [more]
AT5G17710.11.4e-5148.26Co-chaperone GrpE family protein [more]
AT5G17710.21.8e-5146.78Co-chaperone GrpE family protein [more]
AT4G26780.15.0e-0922.96Co-chaperone GrpE family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 117..144
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 287..319
NoneNo IPR availablePANTHERPTHR21237:SF4GRPE PROTEIN HOMOLOGcoord: 1..304
IPR000740GrpE nucleotide exchange factorPRINTSPR00773GRPEPROTEINcoord: 160..176
score: 46.88
coord: 243..258
score: 50.2
coord: 270..289
score: 46.09
coord: 188..203
score: 34.57
IPR000740GrpE nucleotide exchange factorPFAMPF01025GrpEcoord: 134..291
e-value: 8.9E-39
score: 132.8
IPR000740GrpE nucleotide exchange factorPANTHERPTHR21237GRPE PROTEINcoord: 1..304
IPR000740GrpE nucleotide exchange factorPROSITEPS01071GRPEcoord: 246..289
IPR000740GrpE nucleotide exchange factorHAMAPMF_01151GrpEcoord: 77..291
score: 17.129913
IPR000740GrpE nucleotide exchange factorCDDcd00446GrpEcoord: 156..289
e-value: 2.20735E-41
score: 138.793
IPR009012GrpE nucleotide exchange factor, headGENE3D2.30.22.10Head domain of nucleotide exchange factor GrpEcoord: 236..291
e-value: 3.2E-19
score: 70.6
IPR009012GrpE nucleotide exchange factor, headSUPERFAMILY51064Head domain of nucleotide exchange factor GrpEcoord: 238..293
IPR013805GrpE nucleotide exchange factor, coiled-coilGENE3D3.90.20.20coord: 119..232
e-value: 1.9E-20
score: 74.6
IPR013805GrpE nucleotide exchange factor, coiled-coilSUPERFAMILY58014Coiled-coil domain of nucleotide exchange factor GrpEcoord: 141..233

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy01g013250.1Lcy01g013250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006457 protein folding
biological_process GO:0030150 protein import into mitochondrial matrix
biological_process GO:0009408 response to heat
cellular_component GO:0009941 chloroplast envelope
cellular_component GO:0009570 chloroplast stroma
cellular_component GO:0001405 PAM complex, Tim23 associated import motor
molecular_function GO:0000774 adenyl-nucleotide exchange factor activity
molecular_function GO:0051087 chaperone binding
molecular_function GO:0046982 protein heterodimerization activity
molecular_function GO:0042803 protein homodimerization activity
molecular_function GO:0043621 protein self-association
molecular_function GO:0051082 unfolded protein binding