Lcy01g013160 (gene) Sponge gourd (P93075) v1

Overview
NameLcy01g013160
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRNase H domain-containing protein
LocationChr01: 34421220 .. 34421795 (+)
RNA-Seq ExpressionLcy01g013160
SyntenyLcy01g013160
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAATATAGCGAGGCCCAAACACCTAGCCACATTAGTAGGGAAAAATCAAGGCGTGCAGATAATGCCCCTTCCACTGTTTGGGCTTCTCCACCTATCAACCAGTTCAAGCTAAATGTAGATGCAGCAGTCATAGACGGGAACGTGGGTTCGGGAATGATCATTCGAAATCATAAAGGAGAGACGATGGCAGCAGCAGAGTGTTACAGGAAATTAAAATGCTCAGTTGAATGGGCAGAAGCAAAGGCGCTAGTAGATGGTGTTCACCTAGCTCTTGATGCAGGCATATTTCCAATATGGGCAGAAACAGATTCACAAATTGTATGGAATCTTCTGAGCGACCGGGATCAACATTTCAACGAGATTGCTCCTCTTATTCAACACCTAAAGCAATTACAGCTTTCCCACCATATTAGGGGTTTCATGTTAACGCCTAGGGAGGGAAACAGAGTAGCACATACGCTGGCCGTTCATGCTCGCACCACCAAACATGACGAGGTTTGGTTAGAAGATTGTCCTCCATGGGCTCATGGCATCCTTGCTCAAGAGATGCCAACCACAGTTGTCTTACATTAG

mRNA sequence

ATGGAATATAGCGAGGCCCAAACACCTAGCCACATTAGTAGGGAAAAATCAAGGCGTGCAGATAATGCCCCTTCCACTGTTTGGGCTTCTCCACCTATCAACCAGTTCAAGCTAAATGTAGATGCAGCAGTCATAGACGGGAACGTGGGTTCGGGAATGATCATTCGAAATCATAAAGGAGAGACGATGGCAGCAGCAGAGTGTTACAGGAAATTAAAATGCTCAGTTGAATGGGCAGAAGCAAAGGCGCTAGTAGATGGTGTTCACCTAGCTCTTGATGCAGGCATATTTCCAATATGGGCAGAAACAGATTCACAAATTGTATGGAATCTTCTGAGCGACCGGGATCAACATTTCAACGAGATTGCTCCTCTTATTCAACACCTAAAGCAATTACAGCTTTCCCACCATATTAGGGGTTTCATGTTAACGCCTAGGGAGGGAAACAGAGTAGCACATACGCTGGCCGTTCATGCTCGCACCACCAAACATGACGAGGTTTGGTTAGAAGATTGTCCTCCATGGGCTCATGGCATCCTTGCTCAAGAGATGCCAACCACAGTTGTCTTACATTAG

Coding sequence (CDS)

ATGGAATATAGCGAGGCCCAAACACCTAGCCACATTAGTAGGGAAAAATCAAGGCGTGCAGATAATGCCCCTTCCACTGTTTGGGCTTCTCCACCTATCAACCAGTTCAAGCTAAATGTAGATGCAGCAGTCATAGACGGGAACGTGGGTTCGGGAATGATCATTCGAAATCATAAAGGAGAGACGATGGCAGCAGCAGAGTGTTACAGGAAATTAAAATGCTCAGTTGAATGGGCAGAAGCAAAGGCGCTAGTAGATGGTGTTCACCTAGCTCTTGATGCAGGCATATTTCCAATATGGGCAGAAACAGATTCACAAATTGTATGGAATCTTCTGAGCGACCGGGATCAACATTTCAACGAGATTGCTCCTCTTATTCAACACCTAAAGCAATTACAGCTTTCCCACCATATTAGGGGTTTCATGTTAACGCCTAGGGAGGGAAACAGAGTAGCACATACGCTGGCCGTTCATGCTCGCACCACCAAACATGACGAGGTTTGGTTAGAAGATTGTCCTCCATGGGCTCATGGCATCCTTGCTCAAGAGATGCCAACCACAGTTGTCTTACATTAG

Protein sequence

MEYSEAQTPSHISREKSRRADNAPSTVWASPPINQFKLNVDAAVIDGNVGSGMIIRNHKGETMAAAECYRKLKCSVEWAEAKALVDGVHLALDAGIFPIWAETDSQIVWNLLSDRDQHFNEIAPLIQHLKQLQLSHHIRGFMLTPREGNRVAHTLAVHARTTKHDEVWLEDCPPWAHGILAQEMPTTVVLH
Homology
BLAST of Lcy01g013160 vs. ExPASy TrEMBL
Match: A0A5C7H0P0 (Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_022911 PE=4 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 2.1e-18
Identity = 57/163 (34.97%), Postives = 89/163 (54.60%), Query Frame = 0

Query: 28  WASPPINQFKLNVDAA--VIDGNVGSGMIIRNHKGETMAAAECYRKLKCSVEWAEAKALV 87
           W +P   +FK+N DA+  +  G  G G+IIR++KG  +AA         SVE  EA+A +
Sbjct: 273 WKTPKRGEFKINCDASFQLRSGKAGVGVIIRDYKGSAIAARSSPVLCCSSVEMLEAQACL 332

Query: 88  DGVHLALDAGIFPIWAETDSQIVWNLLSDRDQHFNEIAPLIQHLKQLQLSHHIRGFMLTP 147
           +G+HLA+D G+  +  E+D+  V  LLSD+     E+  +I     L  S ++  ++   
Sbjct: 333 EGIHLAIDIGVSGVIIESDAASVIQLLSDQTVPRTELGAIIHTSLALGASVNLLSYVAVR 392

Query: 148 REGNRVAHTLAVHARTTKHDEVWLEDCPPWAHGILAQEMPTTV 189
           RE N VAH +A HA +     VW E+ PP    ++  + PT+V
Sbjct: 393 REANSVAHCIAQHALSLDSPVVWFEEMPPDIARLVRGDSPTSV 435

BLAST of Lcy01g013160 vs. ExPASy TrEMBL
Match: A0A6J1CIF1 (uncharacterized protein LOC111011237 OS=Momordica charantia OX=3673 GN=LOC111011237 PE=4 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 5.1e-17
Identity = 59/175 (33.71%), Postives = 86/175 (49.14%), Query Frame = 0

Query: 1   MEYSEAQTPSHISREKSRRADNAPSTVWASPPINQFKLNVDAAVI--DGNVGSGMIIRNH 60
           ME+ EA      S     R  N    +W  P    +K+N DA+ +  D + G G+IIRN 
Sbjct: 12  MEFREAN-----SNPFPGRVTNTAEVLWLPPDKRIYKINTDASFLASDQHAGLGIIIRND 71

Query: 61  KGETMAAAECYRKLKCSVEWAEAKALVDGVHLALDAGIFPIWAETDSQIVWNLLSDRDQH 120
           +G+ MA+A  Y +   SV+ AEA   V+G+ LA   G+ P+  ETDS  ++NL S   + 
Sbjct: 72  RGQVMASATKYLENIQSVDMAEAIVAVEGLQLASKIGVNPVILETDSSRIFNLFSQPSED 131

Query: 121 FNEIAPLIQHLKQLQLSHHIRGFMLTPREGNRVAHTLAVHARTTKHDEVWLEDCP 174
            +E   ++   K          F    REGN+ AH LA  A   +   +W+ED P
Sbjct: 132 LSETGEIVLKAKNFWTQSLHASFNFVKREGNKAAHMLARRALLLREFSIWMEDWP 181

BLAST of Lcy01g013160 vs. ExPASy TrEMBL
Match: A0A7J9D462 (Uncharacterized protein OS=Gossypium gossypioides OX=34282 GN=Gogos_021255 PE=4 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 2.1e-15
Identity = 54/148 (36.49%), Postives = 73/148 (49.32%), Query Frame = 0

Query: 28  WASPPINQFKLNVDAAVIDGN--VGSGMIIRNHKGETMAAAECYRKLKCSVEWAEAKALV 87
           W+ PP   FK+NVDA V   N  V +  IIRN +G  M +      L  SV  AEA A++
Sbjct: 169 WSPPPQGWFKVNVDAGVSIANNRVVTDFIIRNDEGFIMGSGFQRHNLVLSVVIAEAIAVL 228

Query: 88  DGVHLALDAGIFPIWAETDSQIVWNLLSDRDQHFNEIAPLIQHLKQLQLSHHIRGFMLTP 147
            G+  ALD G   +  E+DS++V N +    + ++E  P    +K L    H   F    
Sbjct: 229 HGLQFALDMGFSRVILESDSRLVVNNIQKSSEDYSESRPFTWDVKNLARKFHCCRFQFVA 288

Query: 148 REGNRVAHTLAVHARTTKHDEVWLEDCP 174
           REGN  AH L V     + D  W+ED P
Sbjct: 289 REGNGAAHALVVEGMQAEGDSFWVEDAP 316

BLAST of Lcy01g013160 vs. ExPASy TrEMBL
Match: A0A2N9EU90 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS6036 PE=4 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 2.1e-15
Identity = 64/174 (36.78%), Postives = 89/174 (51.15%), Query Frame = 0

Query: 4   SEAQTPSHISREKSRRADNAPSTVWASPPINQFKLNVDAAVI-DGN-VGSGMIIRNHKGE 63
           SE Q     S + +R+A       W+ P   ++K+N DAA   D N VG G+IIRNH GE
Sbjct: 485 SEFQEVQESSTQPTRQAVPLQRVTWSPPQPGRYKVNYDAAFFGDSNEVGIGVIIRNHNGE 544

Query: 64  TMAAAECYR-KLKCSVEWAEAKALVDGVHLALDAGIFPIWAETDSQIVWNLLSDRDQHFN 123
            MA+  C+R     SVE  EA A    + +A+D G+  I  E DSQ V + L +    F 
Sbjct: 545 VMASL-CHRIHYPHSVEAMEAYAARSAIQMAIDLGLQAIDIEGDSQTVVDALLNHAPCFT 604

Query: 124 EIAPLIQHLKQLQLSHHIRGFMLTPREGNRVAHTLAVHARTTKHDEVWLEDCPP 175
               LI        S H   F+   R+GN +AH++A  A+ ++  EVW+E  PP
Sbjct: 605 LYGNLINDTNIATQSCHFVQFLHVKRDGNSIAHSIAKRAKFSQPFEVWMESVPP 657

BLAST of Lcy01g013160 vs. ExPASy TrEMBL
Match: A0A6P5S7Q3 (uncharacterized protein LOC110753261 OS=Prunus avium OX=42229 GN=LOC110753261 PE=4 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 8.2e-15
Identity = 55/160 (34.38%), Postives = 82/160 (51.25%), Query Frame = 0

Query: 28  WASPPINQFKLNVDAAVI--DGNVGSGMIIRNHKGETMAAAECYRKLKCSVEWAEAKALV 87
           W+ PP+ ++KLNVDAA I   G  G G ++RN KGE MAA         S + AE  AL 
Sbjct: 59  WSPPPVGRYKLNVDAAFIPETGVSGIGAVVRNDKGEVMAAMALPLANATSSKHAEIMALQ 118

Query: 88  DGVHLALDAGIFPIWAETDSQ-IVWNLLSDRDQHFNEIAPLIQHLKQLQLSHHIRGFMLT 147
            G++ A DAG      E+DSQ +V ++  D ++ +     LI  +K+            +
Sbjct: 119 FGMNFARDAGFSSFLMESDSQGVVNDVKKDEEESWASDGHLIDDIKRSLQHFEEVTISFS 178

Query: 148 PREGNRVAHTLAVHARTTKHDEVWLEDCPPWAHGILAQEM 185
           PR  N+VAH LA HA    +   W+E+ P W   ++  ++
Sbjct: 179 PRRCNQVAHFLARHALNCDNMVTWIEEVPFWLDSVVNDDI 218

BLAST of Lcy01g013160 vs. NCBI nr
Match: TXG50387.1 (hypothetical protein EZV62_022911 [Acer yangbiense])

HSP 1 Score: 102.4 bits (254), Expect = 4.3e-18
Identity = 57/163 (34.97%), Postives = 89/163 (54.60%), Query Frame = 0

Query: 28  WASPPINQFKLNVDAA--VIDGNVGSGMIIRNHKGETMAAAECYRKLKCSVEWAEAKALV 87
           W +P   +FK+N DA+  +  G  G G+IIR++KG  +AA         SVE  EA+A +
Sbjct: 273 WKTPKRGEFKINCDASFQLRSGKAGVGVIIRDYKGSAIAARSSPVLCCSSVEMLEAQACL 332

Query: 88  DGVHLALDAGIFPIWAETDSQIVWNLLSDRDQHFNEIAPLIQHLKQLQLSHHIRGFMLTP 147
           +G+HLA+D G+  +  E+D+  V  LLSD+     E+  +I     L  S ++  ++   
Sbjct: 333 EGIHLAIDIGVSGVIIESDAASVIQLLSDQTVPRTELGAIIHTSLALGASVNLLSYVAVR 392

Query: 148 REGNRVAHTLAVHARTTKHDEVWLEDCPPWAHGILAQEMPTTV 189
           RE N VAH +A HA +     VW E+ PP    ++  + PT+V
Sbjct: 393 REANSVAHCIAQHALSLDSPVVWFEEMPPDIARLVRGDSPTSV 435

BLAST of Lcy01g013160 vs. NCBI nr
Match: XP_030923211.1 (uncharacterized protein LOC115950102 [Quercus lobata])

HSP 1 Score: 99.0 bits (245), Expect = 4.7e-17
Identity = 56/151 (37.09%), Postives = 77/151 (50.99%), Query Frame = 0

Query: 26  TVWASPPINQFKLNVDAAVID--GNVGSGMIIRNHKGETMAAAECYRKLKCSVEWAEAKA 85
           TVW  PP + FKLN DAA+      +G G+II N+KGE MAA    R    S E  E  A
Sbjct: 6   TVWKPPPESAFKLNFDAAIFSELNRIGVGVIIHNYKGEVMAAMSARRPAVHSCEEGELLA 65

Query: 86  LVDGVHLALDAGIFPIWAETDSQIVWNLLSDRDQHFNEIAPLIQHLKQLQLSHHIRGFML 145
               V  A+DAG   +  E  +  V   +S ++ + + +  +++ +K L           
Sbjct: 66  CRKAVEFAIDAGFSRLVLEGGNVNVMQAISSQEANISLLGTVVEDIKHLIRGLQWVSISH 125

Query: 146 TPREGNRVAHTLAVHARTTKHDEVWLEDCPP 175
           T R GN+VAH L  HART+  D  W+ED PP
Sbjct: 126 TRRSGNKVAHVLVQHARTSIEDLYWIEDSPP 156

BLAST of Lcy01g013160 vs. NCBI nr
Match: XP_022140628.1 (uncharacterized protein LOC111011237 [Momordica charantia])

HSP 1 Score: 97.8 bits (242), Expect = 1.1e-16
Identity = 59/175 (33.71%), Postives = 86/175 (49.14%), Query Frame = 0

Query: 1   MEYSEAQTPSHISREKSRRADNAPSTVWASPPINQFKLNVDAAVI--DGNVGSGMIIRNH 60
           ME+ EA      S     R  N    +W  P    +K+N DA+ +  D + G G+IIRN 
Sbjct: 12  MEFREAN-----SNPFPGRVTNTAEVLWLPPDKRIYKINTDASFLASDQHAGLGIIIRND 71

Query: 61  KGETMAAAECYRKLKCSVEWAEAKALVDGVHLALDAGIFPIWAETDSQIVWNLLSDRDQH 120
           +G+ MA+A  Y +   SV+ AEA   V+G+ LA   G+ P+  ETDS  ++NL S   + 
Sbjct: 72  RGQVMASATKYLENIQSVDMAEAIVAVEGLQLASKIGVNPVILETDSSRIFNLFSQPSED 131

Query: 121 FNEIAPLIQHLKQLQLSHHIRGFMLTPREGNRVAHTLAVHARTTKHDEVWLEDCP 174
            +E   ++   K          F    REGN+ AH LA  A   +   +W+ED P
Sbjct: 132 LSETGEIVLKAKNFWTQSLHASFNFVKREGNKAAHMLARRALLLREFSIWMEDWP 181

BLAST of Lcy01g013160 vs. NCBI nr
Match: XP_023899877.1 (uncharacterized protein LOC112011763 [Quercus suber])

HSP 1 Score: 96.7 bits (239), Expect = 2.4e-16
Identity = 60/165 (36.36%), Postives = 86/165 (52.12%), Query Frame = 0

Query: 23  APSTV-WASPPINQFKLNVDAAVI-DGNV-GSGMIIRNHKGETMAAAECYRKLKCSVEWA 82
           AP+ + W  PP + +KLN DAA+  D N  G G IIRN +GE MA       L  +   A
Sbjct: 31  APARISWKPPPTSTYKLNFDAAIFADLNCSGFGAIIRNEEGEVMAGMSVKGPLSLNSAEA 90

Query: 83  EAKALVDGVHLALDAGIFPIWAETDSQIVWNLLSDRDQHFNEIAPLIQHLKQLQLSHHIR 142
           EA A    VH AL+AG   +  E D+ +V N +S   ++ + +  + + ++ L       
Sbjct: 91  EALACRRAVHFALEAGFSRLVIEGDNALVMNAISCSAENNSLLGHIFEDIQHLVRGLQYV 150

Query: 143 GFMLTPREGNRVAHTLAVHARTTKHDEVWLEDCPPWAHGILAQEM 185
                 R+GN VAH+LA HART   +  W+ED PP A   L Q++
Sbjct: 151 SISCIKRDGNMVAHSLARHARTISDEMYWMEDSPPPAVNALYQDL 195

BLAST of Lcy01g013160 vs. NCBI nr
Match: KAG6608327.1 (hypothetical protein SDJN03_01669, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 95.5 bits (236), Expect = 5.2e-16
Identity = 52/140 (37.14%), Postives = 76/140 (54.29%), Query Frame = 0

Query: 28  WASPPINQFKLNVDAAVIDGNVGSGMIIRNHKGETMAAAECYRKLKCSVEWAEAKALVDG 87
           W S P +Q KL+VDA + +  +G  M +                    +  A  K    G
Sbjct: 22  WVSLPHDQDKLSVDATMHNEEMGCQMGL------------------FEIPKALGKDSSRG 81

Query: 88  VHLALDAGIFPIWAETDSQIVWNLLSDRDQHFNEIAPLIQHLKQLQLSHHIRGFMLTPRE 147
             L +  G+  +W + +S++VW LL D+DQHF EIA LI+HLK L  +H I+GF+ TP++
Sbjct: 82  NQLGMATGLTSVWPKVNSKVVWTLLMDQDQHFIEIAHLIKHLKHLSQNHCIKGFLPTPKD 141

Query: 148 GNRVAHTLAVHARTTKHDEV 168
             +  HTLA+HA  TKH E+
Sbjct: 142 DTKATHTLAIHAWHTKHLEI 143

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5C7H0P02.1e-1834.97Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_022911 PE=4 SV=1[more]
A0A6J1CIF15.1e-1733.71uncharacterized protein LOC111011237 OS=Momordica charantia OX=3673 GN=LOC111011... [more]
A0A7J9D4622.1e-1536.49Uncharacterized protein OS=Gossypium gossypioides OX=34282 GN=Gogos_021255 PE=4 ... [more]
A0A2N9EU902.1e-1536.78Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS6036 PE=4 SV=1[more]
A0A6P5S7Q38.2e-1534.38uncharacterized protein LOC110753261 OS=Prunus avium OX=42229 GN=LOC110753261 PE... [more]
Match NameE-valueIdentityDescription
TXG50387.14.3e-1834.97hypothetical protein EZV62_022911 [Acer yangbiense][more]
XP_030923211.14.7e-1737.09uncharacterized protein LOC115950102 [Quercus lobata][more]
XP_022140628.11.1e-1633.71uncharacterized protein LOC111011237 [Momordica charantia][more]
XP_023899877.12.4e-1636.36uncharacterized protein LOC112011763 [Quercus suber][more]
KAG6608327.15.2e-1637.14hypothetical protein SDJN03_01669, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 40..158
e-value: 1.6E-21
score: 76.4
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 34..167
e-value: 6.5E-13
score: 50.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..26
NoneNo IPR availablePANTHERPTHR47723OS05G0353850 PROTEINcoord: 16..184
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 38..156
e-value: 1.35111E-20
score: 80.8212
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 35..161

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy01g013160.1Lcy01g013160.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity