Lcy01g011020 (gene) Sponge gourd (P93075) v1

BLAST of Lcy01g011020 vs. ExPASy Swiss-Prot
Match: Q9FKG6 (U-box domain-containing protein 52 OS=Arabidopsis thaliana OX=3702 GN=PUB52 PE=2 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 1.0e-108
Identity = 266/757 (35.14%), Postives = 398/757 (52.58%), Query Frame = 0

Query: 20  VGIAIDKDKGSQNALKWATENLLIKGQTAILIHVKLRSSGSSLTSPK-VMSATDNGNDNV 79
           V +AI+  K S+  + WA E  + +G T   + + +R   S + +P  +  A     ++V
Sbjct: 24  VAVAINGKKKSKYVVFWALEKFIPEGFTDFKL-LYVRPPVSYIPTPMGIAVAVSELREDV 83

Query: 80  NVLVSKDIDPNTRDLFLPFRCFCTRKDIFCKDIVIEDVDVAKALIEYVSQAGIETLVVGT 139
                +++D +  ++  P++    R+ +  + ++++ ++ A A+ E ++  G+  LV+G 
Sbjct: 84  VSAYKQELDWSANEMLRPYKKMFERRKVQVEVLLLDSLEPAAAIAEEIAGTGVTKLVIGM 143

Query: 140 TTKGGFLRRFKTTDIPGSVIKGAPDFCNVYVISRGKIQTMRSASRPAPTSSPLRSLLLSQ 199
           + +G F R+    D+   +    P FC VYVIS+GK+ ++R +   A             
Sbjct: 144 SLRGFFSRKI---DMSSLIATAVPRFCTVYVISKGKLASVRPSESDA------------- 203

Query: 200 SGS-RSQLSESQISTIQRPEKPP-----IEPARRSQDNIEFRSPFTRKGYNGKMPGEMNL 259
           SGS R + S S   +   P  PP     +     +Q  +   SP  +         +M+ 
Sbjct: 204 SGSIRFERSSSTSGSTDSPRLPPEYQDFLSAVSEAQSRVSPFSPALKHSMGSNAVAQMDT 263

Query: 260 PD--TDISFVSSGR------------PSVDKLFPTFF--DADSGRIPPRLSNGTDGDIYN 319
               TD   VS+GR             + D+ F   F    ++       S   D +   
Sbjct: 264 SSSGTDQEEVSTGRGMEIVHSGIEGKKNKDESFSASFPMGTEAYNSMSWTSKWRDHEDRR 323

Query: 320 SFESQQFGRKSLDVN------YPPEFSSVFSEGDRLSTSSQSMEEV-------EAEMRRL 379
              S         VN       P  +S V      +S    S+  +         E+ +L
Sbjct: 324 EMRSSSSSNNHDLVNMDWGAVVPENYSWVSHTASHMSDGLLSVHSITDNQVNLNFEIEKL 383

Query: 380 KLELKQTMEMYSTACKEALTAKQKAIELQRWKSEEERRIEEARLAEEAALEMVEQEKAKS 439
           + ELK   EMY+ A  E + A +K  EL + + EE  ++ E +  EE A +   +EK + 
Sbjct: 384 RAELKHVQEMYAMAQTETVGASKKLTELNQRRFEESEKLVELKEKEEVAKDTASKEKQRY 443

Query: 440 RAAIEAAEAAKRIAELEAQKRINAEMKAFKEAEEKKKALDALAHNDVRYRKYSIEEIEAA 499
             A++ AE  K +   EA  R  AE KA ++A EK K   +L    V+Y+ Y+ EEI AA
Sbjct: 444 EEAMKEAEKVKELMMKEALHRREAEFKAERDAREKDKLQASLVSPGVQYQHYTWEEIAAA 503

Query: 500 TEFFSESLKIGEGGYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMV 559
           T  F+E+LKIG G YG VYK  L HT  A+KVL     Q   QF QE+E+LS IRHP++V
Sbjct: 504 TSDFAENLKIGIGAYGSVYKCNLHHTTGAVKVLHAGETQLSKQFDQELEILSKIRHPHLV 563

Query: 560 LLLGACPEYGCLVYEFMANGSLDDCLFRRKENSKPLSWQLRFKIAAEIGTSLLFLHQAKP 619
           LLLGACPE GCLVYE+M NGSLDD L     ++ P+ W  RF+IA E+ ++L+FLH++KP
Sbjct: 564 LLLGACPERGCLVYEYMDNGSLDDRLM-LVNDTPPIPWFERFRIALEVASALVFLHKSKP 623

Query: 620 EPLVHRDLKPANILLDRNYVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQ 679
            P++HRDLKP NILLD N+VSK+ DVGL+ +V      S T ++ TS  GT CYIDPEYQ
Sbjct: 624 RPIIHRDLKPGNILLDHNFVSKLGDVGLSTMVNQDDVSSRTIFKQTSPVGTLCYIDPEYQ 683

Query: 680 QTGMLGVKSDIYSLGIMFLQLITAKPPMGLTHHVGRAI-EKGTLLEFLDPSVTDWPAEES 739
           +TG++  KSD+YSLG++ LQLITAKP + +TH V  AI +    +  LD     WP  ++
Sbjct: 684 RTGIISPKSDVYSLGVVILQLITAKPAIAITHMVEEAIGDDAEFMAILDKKAGSWPISDT 743

BLAST of Lcy01g011020 vs. ExPASy Swiss-Prot
Match: Q9FKG5 (U-box domain-containing protein 51 OS=Arabidopsis thaliana OX=3702 GN=PUB51 PE=2 SV=2)

HSP 1 Score: 395.6 bits (1015), Expect = 1.3e-108
Identity = 270/740 (36.49%), Postives = 400/740 (54.05%), Query Frame = 0

Query: 16  GNELVGIAI-DKDKGSQNALKWATENLLIKGQTAI-LIHVKLRSSGSSLTSPKVMSATDN 75
           G  +V +AI   +  ++  ++WA +    +      L+HV+ R S S  T+ K       
Sbjct: 4   GALIVAVAIKGNNSKTKGVVRWALQEFASQEHVVFKLLHVQPRDSNSVSTTRK------- 63

Query: 76  GNDNVNVLVSKDIDPNTRDLFLPFRCFCTRKDIFCKDIVIEDVDVAKALIEYVSQAGIET 135
             D    +  KD+D  TR++ LP R     +++    +V+E  D+A A+ + V   GI  
Sbjct: 64  --DLTTSVYKKDVDRKTREMLLPSRDMFVHREVQLDIMVLESDDIADAISKAVQDHGISE 123

Query: 136 LVVGTTTKGGFLRRFKTTDIPGSVIKGAPDFCNVYVISRGKIQTMRSASRPAPTSSPLRS 195
           LV+G ++   F  + K +++   +    P FC+V+VIS+GK+  +R +     TS     
Sbjct: 124 LVIGASSSIIFSWKLKRSNLSSRIADATPRFCSVHVISKGKLLNVRKSDMDTETS----- 183

Query: 196 LLLSQSGSRSQL-SESQISTIQRPEKPPIEPARRSQDNIEFRSPFTRKGYNGKMPGEMNL 255
             ++   S S+  S+S   T+                 I+  +   +K   G   G+ N 
Sbjct: 184 --IADDRSESRFSSDSHSGTVSSTSSHQFSSTPLLFQRIQALTTVNQK--VGTNIGKQNN 243

Query: 256 PDTDISFVSSGRPSVD--KLF--PTFFDADSGRIPPRLSNGTDGDIYNSFESQQFGRKSL 315
                    +G   VD  KL     F+   S  I      G  G   +S+ S Q    S 
Sbjct: 244 EPHHHHHNRAGSLDVDESKLLNQKGFYRTSSSGI------GYGGSDISSWRSSQMEEAS- 303

Query: 316 DVNYPPEFSSVFSEGDRLSTSSQSMEEVEAEMRRLKLELKQTMEMYSTACKEALTAKQKA 375
                   SS +S+     TSS S    + E+ +LK+EL+    MY+ A  E + A +K 
Sbjct: 304 -------SSSTYSD----PTSSSSQIHKDFELEKLKIELRHIKGMYAVAQSEVIDASKKM 363

Query: 376 IELQRWKSEEERRIEEARLAEEAALEMVEQEKAKSRAAIEAAEAAKRIAELEAQKRINAE 435
            +L + +SEE  R++   + EE A E+VE E+ +   A   AE  +   E E ++R+ AE
Sbjct: 364 QDLNQRRSEEATRLKNLTIREEEADEVVEMERERQEDAENEAELVRECIERETEERLEAE 423

Query: 436 MKAFKEAEEKKKALDALAHNDV---RYRKYSIEEIEAATEFFSESLKIGEGGYGPVYKGY 495
            +A +  +EK++  DAL    +   +Y K+  EEI  AT  FS+ LKIG GGYG VY+  
Sbjct: 424 ARAEEVRKEKQRLEDALEGGPLQRQQYMKFEWEEIVEATSSFSDELKIGVGGYGSVYRCN 483

Query: 496 LDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSL 555
           L HT VA+KVL  D +    QF QE+E+LS IRHP+++LLLGACPE G LVYE+M NGSL
Sbjct: 484 LHHTTVAVKVLHSDKSSLTKQFHQELEILSKIRHPHLLLLLGACPERGSLVYEYMHNGSL 543

Query: 556 DDCLFRRKEN-----SKPLSWQLRFKIAAEIGTSLLFLHQAKPEPLVHRDLKPANILLDR 615
           ++ L +R+ N       PL W  RF+IA EI ++L FLH  +P P+VHRDLKPANILLDR
Sbjct: 544 EERLMKRRPNVDTPQPPPLRWFERFRIAWEIASALYFLHTNEPRPIVHRDLKPANILLDR 603

Query: 616 NYVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIM 675
           N VSKI DVGL+++V    + + T +  T   GTF YIDPEYQ+TG++  +SDIY+ GI+
Sbjct: 604 NNVSKIGDVGLSKMVNLDPSHASTVFNETGPVGTFFYIDPEYQRTGVVTPESDIYAFGII 663

Query: 676 FLQLITAKPPMGLTHHVGRAI--EKGTLLEFLDPSVTDWPAEESLCFAELALKCAELRRK 735
            LQL+TA+  MGL H + +A+  + G   E LD +  DWP +E+     + L+CAE+R++
Sbjct: 664 LLQLVTARSAMGLAHSIEKALRDQTGKFTEILDKTAGDWPVKEAKEMVMIGLRCAEMRKR 707

Query: 736 DRPDLRKVVLPELIRLRTIA 739
           DRPDL K +LP L RL+ +A
Sbjct: 724 DRPDLGKEILPVLERLKEVA 707

BLAST of Lcy01g011020 vs. ExPASy Swiss-Prot
Match: Q9SW11 (U-box domain-containing protein 35 OS=Arabidopsis thaliana OX=3702 GN=PUB35 PE=2 SV=2)

HSP 1 Score: 394.4 bits (1012), Expect = 3.0e-108
Identity = 278/776 (35.82%), Postives = 411/776 (52.96%), Query Frame = 0

Query: 20  VGIAIDKDKGSQNALKWATENLLIKGQTA---ILIHVKLRSSGSSLTSPKVMSATDNGND 79
           V +A+     S+  + WA E    +G      + IH  + S  + + +   +S      D
Sbjct: 22  VVVALSGSSKSKYVVTWAIEKFATEGNVGFKLLHIHPMITSVPTPMGNAIPISEV---RD 81

Query: 80  NVNVLVSKDIDPNTRDLFLPFRCFCTRKDIFCKDIVIEDVDVAKALIEYVSQAGIETLVV 139
           +V     ++I   + ++  P+     R+ +  + +VIE  +VA A+ E V++  I+ +V+
Sbjct: 82  DVVTAYRQEILWQSEEMLKPYTKLFVRRKVAVEVLVIESDNVAAAIAEEVTRDSIDRIVI 141

Query: 140 GTTTKGGFLRRFKTTDIPGSVIKGAPDFCNVYVISRGKIQTMRSASRPAPTS-----SPL 199
           G +++  F R+    DI   +    P+FC VYV+S+GK+  +R +      +     S  
Sbjct: 142 GGSSRSFFSRK---ADICSVISALMPNFCTVYVVSKGKLSCVRPSDSDGNATIREDGSER 201

Query: 200 RSLLLSQSGSRSQLSESQISTIQRPEKPPIEPARRSQDNIEFRSPFTRKGYNGKMPGEMN 259
            +     SG  S  S+   S      +P   P RR Q        F        +P E +
Sbjct: 202 TNSSSGSSGPTSDSSDVMSSAHDSQSRPLSLPVRRMQH-------FPAIAGQASVPMETS 261

Query: 260 LPDTD----ISFVSSGRPSVDKLFPTFFDADSGRIPPR---------LSNGTDGDIYNSF 319
              +D    +S  +     V  +  +  D  S   P R         +S+ +    Y +F
Sbjct: 262 SVGSDETRCMSLDAEEARDVSSINRSSTDTTSRWTPRRRDYEERKEAMSSSSSNREYGNF 321

Query: 320 ESQ-QFGRKSLDVNYPPEFSSVFSEGDRLSTSSQSMEEVEA--EMRRLKLELKQTMEMYS 379
            ++  +    +D  +        +  D LS  S +  +V    E+ +L+ EL+   EMY+
Sbjct: 322 GTRFSWSGMGVDTTHSRASQQASNMSDALSEQSYTDNQVNLNFEVEKLRAELRHVQEMYA 381

Query: 380 TACKEALTAKQKAIELQRWKSEEERRIEEARLAEEAALEMVEQEKAKSRAAIEAAEAAKR 439
            A  E   A +K  EL + + EE  ++EE +L E  A E+ E+EK     A   AE+ + 
Sbjct: 382 VAQTETFDASRKLGELNQRRLEEAIKLEELKLKEYEARELAEKEKQNFEKARRDAESMRE 441

Query: 440 IAELEAQKRINAEMKAFKEAEEKKKALDALAHNDVRYRKYSIEEIEAATEFFSESLKIGE 499
            AE E  +R  AE K+ ++ +EK+K    L    ++Y+ ++ EEI AAT  FSE LKIG 
Sbjct: 442 RAEREIAQRREAERKSARDTKEKEKLEGTLGSPQLQYQHFAWEEIMAATSSFSEELKIGM 501

Query: 500 GGYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGCL 559
           G YG VYK  L HT   +KVL+    Q   QFQQE+E+LS IRHP++VLLLGACPE G L
Sbjct: 502 GAYGAVYKCNLHHTTAVVKVLQSAENQLSKQFQQELEILSKIRHPHLVLLLGACPEQGAL 561

Query: 560 VYEFMANGSLDDCLFRRKENSKPLSWQLRFKIAAEIGTSLLFLHQAKPEPLVHRDLKPAN 619
           VYE+M NGSL+D LF +  NS PL W  RF+IA E+  +L+FLH++KP+P++HRDLKPAN
Sbjct: 562 VYEYMENGSLEDRLF-QVNNSPPLPWFERFRIAWEVAAALVFLHKSKPKPIIHRDLKPAN 621

Query: 620 ILLDRNYVSKISDVGLARLVP-PSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDI 679
           ILLD N+VSK+ DVGL+ +V    ++   T Y+ TS  GT CYIDPEYQ+TG +  KSDI
Sbjct: 622 ILLDHNFVSKVGDVGLSTMVQVDPLSTKFTIYKQTSPVGTLCYIDPEYQRTGRISSKSDI 681

Query: 680 YSLGIMFLQLITAKPPMGLTHHVGRAIEKG-TLLEFLDPSVTDWPAEESLCFAELALKCA 739
           YS G++ LQL+TAKP + LTH V  A++     L+ LD    +WP EE+   A LAL C 
Sbjct: 682 YSFGMILLQLLTAKPAIALTHFVESAMDSNDEFLKILDQKAGNWPIEETRELAALALCCT 741

Query: 740 ELRRKDRPDLRKVVLPELIRLRTIAEASMYSSVTDGVSSPEFSNSQIRLKTDVSGE 770
           ELR KDRPDL+  +LP L  L+ +AE +  S    GVS+   ++    L  DV  E
Sbjct: 742 ELRGKDRPDLKDQILPALENLKKVAEKARNS--FSGVSTQPPTHFICPLLKDVMNE 781

BLAST of Lcy01g011020 vs. ExPASy Swiss-Prot
Match: Q8S8S7 (U-box domain-containing protein 34 OS=Arabidopsis thaliana OX=3702 GN=PUB34 PE=3 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 1.9e-107
Identity = 276/742 (37.20%), Postives = 389/742 (52.43%), Query Frame = 0

Query: 6   PVKEKKEVVTGNELVGIAIDK--DKGSQNALKWATENLLIKGQTAILIHVKLRSSGSSLT 65
           P  E+ ++     + G+  DK    GS+ A++WA +NLL K    ++IHV          
Sbjct: 15  PKAEEGQLFVAVAVKGLIGDKLGGAGSRRAVRWAVDNLLPKADKFVMIHV---------- 74

Query: 66  SPKVMSATDNGNDNVNVLVSKDIDPNTRDLFLPFRCFCTRKDIFCKDIVIEDVDVAKALI 125
              + + T     N+ +L+   +   T    LP        +    ++ + DV   K   
Sbjct: 75  ---IPTITSIPTPNILILMFTRMWVVTAGDRLPVE----EVEESVVEMYVRDV---KKEY 134

Query: 126 EYVSQAGIETLVVGTTTKGGF-LRRFKTTDIPGSVIKGAPDFCNVYVISRGKIQT--MRS 185
           E V    ++      +TK  F  RR K T +P +V++ AP+ C VY++ + +I T  M  
Sbjct: 135 ETVFVPFLKMCKSTRSTKRYFRSRRTKGTGVPLTVLRYAPETCEVYIVCKDRITTKSMDP 194

Query: 186 ASRPAPTSSPLRSLLLSQSGSRSQLSESQISTIQRPEKPPIEPARRSQDNIEFRSPFTRK 245
                P +SP        + +         ++      P +   R+S +    RS   R+
Sbjct: 195 LINREPCTSP------HAAATAHDFLRDWAASFHTLRSPTLPDPRQSTEAGTRRSASARE 254

Query: 246 GYNGKMPGEMNLPDTDISFVSSGRPSVDKLFPTFFDADSGRIPPRLSNGTDGDIYNSFES 305
                +    N P T  S  +S   +     P  F    G   P+L+       Y+ F+ 
Sbjct: 255 LRFEALSLTCNKPKTPQSSKASSATT-----PEIFRRRRGSDIPQLN-------YSDFDK 314

Query: 306 QQFGRKSLDVNYPPEFSSVFSEGDRLSTSSQSMEEVEAEMRRLKLELKQTMEMYSTACKE 365
                +S   N   E           S  S+ + E+E E+ RLK EL+ T+  Y  AC+E
Sbjct: 315 TCTKPQSNVENIVSEHRDSDRSPPETSRKSKKV-EIEEEVERLKNELQSTVFKYKQACEE 374

Query: 366 ALTAKQKAIELQRWKSEEERRIEEARLAEEAALEMVEQEKAKSRAAIEAAEAAKRIAELE 425
             + + K   L      E +R+  A   EE        EK +   A++  E AK +   E
Sbjct: 375 LFSTQNKVKMLSTEYLNESKRVNNAVEKEELQRNTAALEKERYMKAVKEVETAKALLARE 434

Query: 426 AQKRINAEMKAFKEAEEKKKALDALAHNDVRYRKYSIEEIEAATEFFSESLKIGEGGYGP 485
             +R  AE+ A +   EKKK +D L   D RYRKY+IEEI  ATE FS    IGEGGYG 
Sbjct: 435 FCQRQIAEVNALRTYLEKKKVIDQLLGTDHRYRKYTIEEIVTATEGFSPEKVIGEGGYGK 494

Query: 486 VYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFM 545
           VY+  LD TP A+KV+R D  + + +F +EVEVLS +RHP++VLLLGACPE GCLVYE++
Sbjct: 495 VYQCSLDSTPAAVKVVRLDTPEKKQEFLKEVEVLSQLRHPHVVLLLGACPENGCLVYEYL 554

Query: 546 ANGSLDDCLFRRKENSKPLSWQLRFKIAAEIGTSLLFLHQAKPEPLVHRDLKPANILLDR 605
            NGSL++ +F RK N  PL W +RF++  E+   L FLH +KPEP+VHRDLKP NILL+R
Sbjct: 555 ENGSLEEYIFHRK-NKPPLPWFIRFRVIFEVACGLAFLHSSKPEPIVHRDLKPGNILLNR 614

Query: 606 NYVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIM 665
           NYVSKI+DVGLA+LV     D+VT YR +  AGT  YIDPEY +TG +  KSD+Y+ GI+
Sbjct: 615 NYVSKIADVGLAKLVTDVAPDNVTMYRNSVLAGTLHYIDPEYHRTGTIRPKSDLYAFGII 674

Query: 666 FLQLITAKPPMGLTHHVGRAIEKGTLLEFLDPSVTDWPAEESLCFAELALKCAELRRKDR 725
            LQL+TA+ P G+   V  A++KGTL E LD SVTDWP  E+   A + LKCAE R +DR
Sbjct: 675 ILQLLTARNPSGIVPAVENAVKKGTLTEMLDKSVTDWPLAETEELARIGLKCAEFRCRDR 716

Query: 726 PDLRKVVLPELIRLRTIAEASM 743
           PDL+  V+P L RL   A + +
Sbjct: 735 PDLKSEVIPVLKRLVETANSKV 716

BLAST of Lcy01g011020 vs. ExPASy Swiss-Prot
Match: Q9LU47 (Putative U-box domain-containing protein 53 OS=Arabidopsis thaliana OX=3702 GN=PUB53 PE=3 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 1.4e-94
Identity = 254/749 (33.91%), Postives = 385/749 (51.40%), Query Frame = 0

Query: 20  VGIAIDKDKGSQNALKWATENL-LIKGQTAILIHVKLRSSGSSLTSPKVMSATDNGNDNV 79
           V +AI     S+N +KWA       K  T  LIH+  + +     S  ++S ++   +  
Sbjct: 34  VALAISGSIKSKNVIKWALNKFGSDKNVTFKLIHIHPKITTLPTASGNIVSISEELEEVA 93

Query: 80  NVLVSKDIDPNTRDLFLPFRCFCTRKDIFCKDI--------------VIEDVDVAKALIE 139
                K +      L  PF+  C RK +   +               V+E   VA A+ +
Sbjct: 94  AAYRQKVMQETKETLLKPFKKMCERKKLKIDETRFESSLTKVAVELQVLESNSVAVAITK 153

Query: 140 YVSQAGIETLVVGTTTKGGFLRRFKTTDIPGSVIKGAPDFCNVYVISRGKIQTM-RSASR 199
            V+Q  I  L++G +++    R +   DI  S+     + C VYV+S G +  + +  S 
Sbjct: 154 EVNQHLISNLIIGRSSQAASSRNY---DITASISASVSNLCTVYVVSNGGVHILAKDTSD 213

Query: 200 PAPTSSPLRSLLLSQSGSRSQLSESQISTIQRPEKP-PIEPARRSQDNIEFRSPFTRKGY 259
                + + S     S S S  S +    +    K  P   + +   N+    P   +G 
Sbjct: 214 TERNDTSIESGFERTSSSCSSGSGANSDVMSNALKSNPHTLSNKRMQNL----PTIVRGV 273

Query: 260 NGKMPGEMNLPDTDISFVSSGRPSVDKLFPTFFDADSGRIPPRLSNGTD-GDIYNSFESQ 319
           +  +P E +  ++D            K      +  S R  P  S        +  F+ +
Sbjct: 274 S--VPMETSSTESD---------ETKKRSSDAAEEASKRSSPETSRSVSWNPQFRDFDER 333

Query: 320 QFGRKSLDVNYPPEFSSVFSEGDRLSTSSQSMEEVEAEMRRLKLELKQTMEMYSTACKEA 379
           +    S+  N+  E+ +V +      T +Q   +   E+ +L+ EL+   EMY+ A  E 
Sbjct: 334 KDAMSSMSSNF--EYGNVVTPLGHYFTDNQ---DTLNEISKLRAELRHAHEMYAVAQVET 393

Query: 380 LTAKQKAIELQRWKSEEERRIEEARLAEEAALEMVEQEKAKSRAAIEAAEAAKRIAELEA 439
           L A +K  EL         + EE  L E     + ++E  K           KR  E EA
Sbjct: 394 LDASRKLNEL---------KFEELTLLEHETKGIAKKETEKFE--------QKRREEREA 453

Query: 440 QKRINAEMKAFKEAEEKKKALD-ALAHNDVRYRKYSIEEIEAATEFFSESLKIGEGGYGP 499
            +R  AEMKA  EA+EK+K  + +L    ++Y++++ EEI  AT  FSE LKIG G YG 
Sbjct: 454 AQRREAEMKATHEAKEKEKLEESSLVAPKLQYQEFTWEEIINATSSFSEDLKIGMGAYGD 513

Query: 500 VYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFM 559
           VYK  L HT  A+KVL    +    QF QE+E+LS IRHP++VLLLGACP++G LVYE+M
Sbjct: 514 VYKCNLHHTIAAVKVLHSAESSLSKQFDQELEILSKIRHPHLVLLLGACPDHGALVYEYM 573

Query: 560 ANGSLDDCLFRRKENSKPLSWQLRFKIAAEIGTSLLFLHQAKPEPLVHRDLKPANILLDR 619
            NGSL+D LF +  +S+P+ W +R +IA E+ ++L+FLH++KP P++HRDLKPANILL+ 
Sbjct: 574 ENGSLEDRLF-QVNDSQPIPWFVRLRIAWEVASALVFLHKSKPTPIIHRDLKPANILLNH 633

Query: 620 NYVSKISDVGLARLVPPS--VADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLG 679
           N+VSK+ DVGL+ ++  +  ++   T Y+ TS  GT CYIDPEYQ+TG +  KSD+Y+ G
Sbjct: 634 NFVSKVGDVGLSTMIQAADPLSTKFTMYKQTSPVGTLCYIDPEYQRTGRISPKSDVYAFG 693

Query: 680 IMFLQLITAKPPMGLTHHVGRAIEKGT---LLEFLDPSVTDWPAEESLCFAELALKCAEL 739
           ++ LQL+T +  M LT+ V  A+E      L++ LD    +WP EE+   A LAL+C EL
Sbjct: 694 MIILQLLTGQQAMALTYTVETAMENNNDDELIQILDEKAGNWPIEETRQLAALALQCTEL 741

Query: 740 RRKDRPDLRKVVLPELIRLRTIAEASMYS 745
           R KDRPDL   +LP L  L+ +A+ +  S
Sbjct: 754 RSKDRPDLEDQILPVLESLKKVADKARNS 741

BLAST of Lcy01g011020 vs. ExPASy TrEMBL
Match: A0A6J1BTT1 (U-box domain-containing protein 35-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111004731 PE=4 SV=1)

HSP 1 Score: 1297.7 bits (3357), Expect = 0.0e+00
Identity = 675/783 (86.21%), Postives = 720/783 (91.95%), Query Frame = 0

Query: 1   MWNQKPVKEKKEVVTGNELVGIAIDKDKGSQNALKWATENLLIKGQTAILIHVKLRSSGS 60
           MWNQK  KE KE  TGNELV IAIDKDKGSQNALKWATENLL+KGQTAILIHVKL+SS +
Sbjct: 1   MWNQKQTKE-KEGATGNELVAIAIDKDKGSQNALKWATENLLLKGQTAILIHVKLKSSSN 60

Query: 61  S---LTSPKVMSATDNGNDNVNVLVSKDIDPNTRDLFLPFRCFCTRKDIFCKDIVIEDVD 120
           S   + SPKV SATDN N   N L+ KD+DP TRDLFLPFRCFCTRKDIFCKDI++ED D
Sbjct: 61  STSYMASPKVTSATDNANG--NFLIGKDLDPVTRDLFLPFRCFCTRKDIFCKDIILEDTD 120

Query: 121 VAKALIEYVSQAGIETLVVGTTTKGGFLRRFKTTDIPGSVIKGAPDFCNVYVISRGKIQT 180
           V+KAL+EYVSQA IE LVVGTTTK GFLRRFKTTDIPGSVIKGAPDFC VYVISRGKIQT
Sbjct: 121 VSKALLEYVSQAAIENLVVGTTTKNGFLRRFKTTDIPGSVIKGAPDFCTVYVISRGKIQT 180

Query: 181 MRSASRPAPTSSPLRSLLLSQSGSRSQLSESQISTIQRPEKPPIEPARRSQDNIEFRSPF 240
           MRSASRPAPTSSPLRS LL+QSG +S LSESQ+S IQR EKPP+E ARRSQDNIEFRSPF
Sbjct: 181 MRSASRPAPTSSPLRSFLLNQSGIKSPLSESQVSQIQRIEKPPLEAARRSQDNIEFRSPF 240

Query: 241 TRKGYNGKMPGEMNLPDTDISFVSSGRPSVDKLFPTFFDA-DSGRIPPRLSNGTDGDIYN 300
           TRKGYNGKMPGEM+LPDTDISFVSSGRPSVD+LFP++FD  DSGR+ PRLS+GT+ D+YN
Sbjct: 241 TRKGYNGKMPGEMSLPDTDISFVSSGRPSVDRLFPSYFDTPDSGRVTPRLSSGTEADMYN 300

Query: 301 SFESQQFGRKSLDVNYPPEFSSVFSEGDRLSTSSQSMEEVEAEMRRLKLELKQTMEMYST 360
           SFESQ FGR+S+DVNYPPEF SVF +GDRLSTSSQ+ME+VEAEMRRLKLELKQTMEMYST
Sbjct: 301 SFESQPFGRRSVDVNYPPEFLSVFPDGDRLSTSSQNMEDVEAEMRRLKLELKQTMEMYST 360

Query: 361 ACKEALTAKQKAIELQRWKSEEERRIEEARLAEEAALEMVEQEKAKSRAAIEAAEAAKRI 420
           ACKEALTAKQKA+ELQRWKSEEERR+EEARLAEEAAL +VEQEKAKS+AAIEAAEAAKRI
Sbjct: 361 ACKEALTAKQKAVELQRWKSEEERRLEEARLAEEAALAIVEQEKAKSQAAIEAAEAAKRI 420

Query: 421 AELEAQKRINAEMKAFKEAEEKKKALDALAHNDVRYRKYSIEEIEAATEFFSESLKIGEG 480
           AELEAQKRINAE+KAFKEAEEKKKALDALAH DVRYRKYSIEEIEAATEFFSESLKIGEG
Sbjct: 421 AELEAQKRINAELKAFKEAEEKKKALDALAHTDVRYRKYSIEEIEAATEFFSESLKIGEG 480

Query: 481 GYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGCLV 540
           GYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGCLV
Sbjct: 481 GYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGCLV 540

Query: 541 YEFMANGSLDDCLFRRKENSKPLSWQLRFKIAAEIGTSLLFLHQAKPEPLVHRDLKPANI 600
           YEFMANGSLDDCLF R+EN KPLSWQLRFKIAAEIGTSLLFLHQ KPEPLVHRDLKPANI
Sbjct: 541 YEFMANGSLDDCLFHRRENLKPLSWQLRFKIAAEIGTSLLFLHQTKPEPLVHRDLKPANI 600

Query: 601 LLDRNYVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYS 660
           LLDRN+VSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYS
Sbjct: 601 LLDRNFVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYS 660

Query: 661 LGIMFLQLITAKPPMGLTHHVGRAIEKGTLLEFLDPSVTDWPAEESLCFAELALKCAELR 720
           LGIMFLQLITAKPPMGLTHHV RAIEKG   E LD SVTDWPAEE+LCFA+LALKCAELR
Sbjct: 661 LGIMFLQLITAKPPMGLTHHVERAIEKGKFSELLDQSVTDWPAEETLCFAQLALKCAELR 720

Query: 721 RKDRPDLRKVVLPELIRLRTIAEASMYSSVTDGVSSPEFSNSQI-RLKTDVSGESFSSRS 779
           RKDRPDLRKVVLPELIR R++AEA+    + DGV+SP FS SQ+   K D+SG+  SS+S
Sbjct: 721 RKDRPDLRKVVLPELIRFRSLAEANTSPPMADGVTSPVFSRSQVSAFKQDLSGDRVSSQS 780

BLAST of Lcy01g011020 vs. ExPASy TrEMBL
Match: A0A6J1BQN6 (U-box domain-containing protein 35-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111004731 PE=4 SV=1)

HSP 1 Score: 1290.8 bits (3339), Expect = 0.0e+00
Identity = 674/783 (86.08%), Postives = 719/783 (91.83%), Query Frame = 0

Query: 1   MWNQKPVKEKKEVVTGNELVGIAIDKDKGSQNALKWATENLLIKGQTAILIHVKLRSSGS 60
           MWNQK  KE KE  TGNELV IAIDKDKGSQNALKWATENLL+KGQTAILIHVKL+SS +
Sbjct: 1   MWNQKQTKE-KEGATGNELVAIAIDKDKGSQNALKWATENLLLKGQTAILIHVKLKSSSN 60

Query: 61  S---LTSPKVMSATDNGNDNVNVLVSKDIDPNTRDLFLPFRCFCTRKDIFCKDIVIEDVD 120
           S   + SPKV SATDN N   N L+ KD+DP TRDLFLPFRCFCTRKDIFCKDI++ED D
Sbjct: 61  STSYMASPKVTSATDNANG--NFLIGKDLDPVTRDLFLPFRCFCTRKDIFCKDIILEDTD 120

Query: 121 VAKALIEYVSQAGIETLVVGTTTKGGFLRRFKTTDIPGSVIKGAPDFCNVYVISRGKIQT 180
           V+KAL+EYVSQA IE LVVGTTTK GFL RFKTTDIPGSVIKGAPDFC VYVISRGKIQT
Sbjct: 121 VSKALLEYVSQAAIENLVVGTTTKNGFL-RFKTTDIPGSVIKGAPDFCTVYVISRGKIQT 180

Query: 181 MRSASRPAPTSSPLRSLLLSQSGSRSQLSESQISTIQRPEKPPIEPARRSQDNIEFRSPF 240
           MRSASRPAPTSSPLRS LL+QSG +S LSESQ+S IQR EKPP+E ARRSQDNIEFRSPF
Sbjct: 181 MRSASRPAPTSSPLRSFLLNQSGIKSPLSESQVSQIQRIEKPPLEAARRSQDNIEFRSPF 240

Query: 241 TRKGYNGKMPGEMNLPDTDISFVSSGRPSVDKLFPTFFDA-DSGRIPPRLSNGTDGDIYN 300
           TRKGYNGKMPGEM+LPDTDISFVSSGRPSVD+LFP++FD  DSGR+ PRLS+GT+ D+YN
Sbjct: 241 TRKGYNGKMPGEMSLPDTDISFVSSGRPSVDRLFPSYFDTPDSGRVTPRLSSGTEADMYN 300

Query: 301 SFESQQFGRKSLDVNYPPEFSSVFSEGDRLSTSSQSMEEVEAEMRRLKLELKQTMEMYST 360
           SFESQ FGR+S+DVNYPPEF SVF +GDRLSTSSQ+ME+VEAEMRRLKLELKQTMEMYST
Sbjct: 301 SFESQPFGRRSVDVNYPPEFLSVFPDGDRLSTSSQNMEDVEAEMRRLKLELKQTMEMYST 360

Query: 361 ACKEALTAKQKAIELQRWKSEEERRIEEARLAEEAALEMVEQEKAKSRAAIEAAEAAKRI 420
           ACKEALTAKQKA+ELQRWKSEEERR+EEARLAEEAAL +VEQEKAKS+AAIEAAEAAKRI
Sbjct: 361 ACKEALTAKQKAVELQRWKSEEERRLEEARLAEEAALAIVEQEKAKSQAAIEAAEAAKRI 420

Query: 421 AELEAQKRINAEMKAFKEAEEKKKALDALAHNDVRYRKYSIEEIEAATEFFSESLKIGEG 480
           AELEAQKRINAE+KAFKEAEEKKKALDALAH DVRYRKYSIEEIEAATEFFSESLKIGEG
Sbjct: 421 AELEAQKRINAELKAFKEAEEKKKALDALAHTDVRYRKYSIEEIEAATEFFSESLKIGEG 480

Query: 481 GYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGCLV 540
           GYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGCLV
Sbjct: 481 GYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGCLV 540

Query: 541 YEFMANGSLDDCLFRRKENSKPLSWQLRFKIAAEIGTSLLFLHQAKPEPLVHRDLKPANI 600
           YEFMANGSLDDCLF R+EN KPLSWQLRFKIAAEIGTSLLFLHQ KPEPLVHRDLKPANI
Sbjct: 541 YEFMANGSLDDCLFHRRENLKPLSWQLRFKIAAEIGTSLLFLHQTKPEPLVHRDLKPANI 600

Query: 601 LLDRNYVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYS 660
           LLDRN+VSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYS
Sbjct: 601 LLDRNFVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYS 660

Query: 661 LGIMFLQLITAKPPMGLTHHVGRAIEKGTLLEFLDPSVTDWPAEESLCFAELALKCAELR 720
           LGIMFLQLITAKPPMGLTHHV RAIEKG   E LD SVTDWPAEE+LCFA+LALKCAELR
Sbjct: 661 LGIMFLQLITAKPPMGLTHHVERAIEKGKFSELLDQSVTDWPAEETLCFAQLALKCAELR 720

Query: 721 RKDRPDLRKVVLPELIRLRTIAEASMYSSVTDGVSSPEFSNSQI-RLKTDVSGESFSSRS 779
           RKDRPDLRKVVLPELIR R++AEA+    + DGV+SP FS SQ+   K D+SG+  SS+S
Sbjct: 721 RKDRPDLRKVVLPELIRFRSLAEANTSPPMADGVTSPVFSRSQVSAFKQDLSGDRVSSQS 779

BLAST of Lcy01g011020 vs. ExPASy TrEMBL
Match: A0A6J1KKQ7 (U-box domain-containing protein 52-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111496144 PE=4 SV=1)

HSP 1 Score: 1280.4 bits (3312), Expect = 0.0e+00
Identity = 670/755 (88.74%), Postives = 707/755 (93.64%), Query Frame = 0

Query: 1   MWNQKPVKEKKE-VVTGNELVGIAIDKDKGSQNALKWATENLLIKGQTAILIHVKLRS-- 60
           MW QK  KE+KE VV+GN+LVGIAIDKDKGSQNALKWATENLL KGQTAIL+HVKLRS  
Sbjct: 73  MWTQKYAKERKEVVVSGNQLVGIAIDKDKGSQNALKWATENLLQKGQTAILVHVKLRSSS 132

Query: 61  -SGSSLTSPKVMSATDNGNDNVNVLVSKDIDPNTRDLFLPFRCFCTRKDIFCKDIVIEDV 120
            SGSS  S  +MS   +G    NVLVS +IDP+TRDLFLPFRCFCTRKDIFCKDIVIEDV
Sbjct: 133 HSGSSYASSDLMSNNGSG----NVLVSNEIDPSTRDLFLPFRCFCTRKDIFCKDIVIEDV 192

Query: 121 DVAKALIEYVSQAGIETLVVGTTTKGGFLRRFKTTDIPGSVIKGAPDFCNVYVISRGKIQ 180
           DVAKALIEYV+QAGI+TL+VGTTTK GFL RFKTTDIPG+++KGAPDFCNVYVISRGKIQ
Sbjct: 193 DVAKALIEYVTQAGIDTLIVGTTTKSGFL-RFKTTDIPGTILKGAPDFCNVYVISRGKIQ 252

Query: 181 TMRSASRPAPTSSPLRSLLLSQSGSRSQLSESQISTIQRPEKPPIEPARRSQDNIEFRSP 240
           TMRSASRPAPTSSPLR+ LLSQSG +SQ S+SQ   IQRPEKPPIEPARRS +NIEFRSP
Sbjct: 253 TMRSASRPAPTSSPLRNFLLSQSGIKSQSSDSQTPQIQRPEKPPIEPARRSHENIEFRSP 312

Query: 241 FTRKGYNGKMPGEMNLPDTDISFVSSGRPSVDKLFPTFFDA-DSGRIPPRLSNGTDGDIY 300
           FTRKGYNGKMPGEM++PDTDISFV SGRPSVDKLFPT++DA D GR+PPRLSNGTDGDIY
Sbjct: 313 FTRKGYNGKMPGEMSVPDTDISFVGSGRPSVDKLFPTYYDAVDPGRMPPRLSNGTDGDIY 372

Query: 301 NSFESQQFGRKSLDVNYPPEFSSVFSEGDRLSTSSQSMEEVEAEMRRLKLELKQTMEMYS 360
           +SFESQ FGRKSLDVN+  EFSS+FSEG+RLSTSSQSMEEVEAEMRRLKLELKQTMEMYS
Sbjct: 373 HSFESQPFGRKSLDVNF-QEFSSIFSEGERLSTSSQSMEEVEAEMRRLKLELKQTMEMYS 432

Query: 361 TACKEALTAKQKAIELQRWKSEEERRIEEARLAEEAALEMVEQEKAKSRAAIEAAEAAKR 420
           TACKEALTAKQKAIELQRWKSEEERRIEEARLAEEAAL MVEQEKAKSRAA+EAAEA+KR
Sbjct: 433 TACKEALTAKQKAIELQRWKSEEERRIEEARLAEEAALAMVEQEKAKSRAAMEAAEASKR 492

Query: 421 IAELEAQKRINAEMKAFKEAEEKKKALDALAHNDVRYRKYSIEEIEAATEFFSESLKIGE 480
           IAELEAQKRINAE+KAFKEAEEKKKALDALA+NDVRYR+Y+IEEIEAATEFFSESLKIGE
Sbjct: 493 IAELEAQKRINAEIKAFKEAEEKKKALDALANNDVRYRRYTIEEIEAATEFFSESLKIGE 552

Query: 481 GGYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGCL 540
           GGYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGCL
Sbjct: 553 GGYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGCL 612

Query: 541 VYEFMANGSLDDCLFRRKENSKPLSWQLRFKIAAEIGTSLLFLHQAKPEPLVHRDLKPAN 600
           VYEFMANGSLDDCLFRRKE SKPLSWQLRFKIAAEIGTSLLFLHQ KPEPLVHRDLKPAN
Sbjct: 613 VYEFMANGSLDDCLFRRKEKSKPLSWQLRFKIAAEIGTSLLFLHQTKPEPLVHRDLKPAN 672

Query: 601 ILLDRNYVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIY 660
           ILLD NYVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIY
Sbjct: 673 ILLDFNYVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIY 732

Query: 661 SLGIMFLQLITAKPPMGLTHHVGRAIEKGTLLEFLDPSVTDWPAEESLCFAELALKCAEL 720
           SLGIMFLQLITAKPPMGLTHHVGRAIEKG L E LDPSV DWPAEE+LCFAELALKCAEL
Sbjct: 733 SLGIMFLQLITAKPPMGLTHHVGRAIEKGNLSEVLDPSVLDWPAEETLCFAELALKCAEL 792

Query: 721 RRKDRPDLRKVVLPELIRLRTIAEASMYSSVTDGV 751
           RRKDRPDL   VLPELIRLR++AEAS+YSS  DGV
Sbjct: 793 RRKDRPDLSMTVLPELIRLRSVAEASLYSSDKDGV 821

BLAST of Lcy01g011020 vs. ExPASy TrEMBL
Match: A0A6J1GHK5 (U-box domain-containing protein 52-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111454244 PE=4 SV=1)

HSP 1 Score: 1276.9 bits (3303), Expect = 0.0e+00
Identity = 668/755 (88.48%), Postives = 706/755 (93.51%), Query Frame = 0

Query: 1   MWNQKPVKEKKE-VVTGNELVGIAIDKDKGSQNALKWATENLLIKGQTAILIHVKLRS-- 60
           MW QK  KE+KE VV  N+LVGIAIDKDKGSQNALKWATENLL KGQTAILIHVKLRS  
Sbjct: 1   MWTQKYAKERKEVVVASNQLVGIAIDKDKGSQNALKWATENLLQKGQTAILIHVKLRSSS 60

Query: 61  -SGSSLTSPKVMSATDNGNDNVNVLVSKDIDPNTRDLFLPFRCFCTRKDIFCKDIVIEDV 120
            SGSS  S  +MS   +G    NVLVS +IDP+TRDLFLPFRCFCTRKDIFCKDIVIEDV
Sbjct: 61  HSGSSYASSDLMSNNGSG----NVLVSNEIDPSTRDLFLPFRCFCTRKDIFCKDIVIEDV 120

Query: 121 DVAKALIEYVSQAGIETLVVGTTTKGGFLRRFKTTDIPGSVIKGAPDFCNVYVISRGKIQ 180
           DVAKALIEYV+QAGI+TL+VGTTTK GFL RFKTTDIPG+++KGAPDFCNVYVISRGKIQ
Sbjct: 121 DVAKALIEYVTQAGIDTLIVGTTTKSGFL-RFKTTDIPGTILKGAPDFCNVYVISRGKIQ 180

Query: 181 TMRSASRPAPTSSPLRSLLLSQSGSRSQLSESQISTIQRPEKPPIEPARRSQDNIEFRSP 240
           TMRSASRPAPTSSPLR+ LL+QSG +SQ S+SQ   IQRPEKPPIEPARRS +NIEFRSP
Sbjct: 181 TMRSASRPAPTSSPLRNFLLNQSGIKSQSSDSQTPQIQRPEKPPIEPARRSHENIEFRSP 240

Query: 241 FTRKGYNGKMPGEMNLPDTDISFVSSGRPSVDKLFPTFFDA-DSGRIPPRLSNGTDGDIY 300
           FTRKGYNGKMPGEM++PDTDISFV SGRPSVDKLFPT++DA D GR+PPRLSNGTDGDIY
Sbjct: 241 FTRKGYNGKMPGEMSVPDTDISFVGSGRPSVDKLFPTYYDAVDPGRMPPRLSNGTDGDIY 300

Query: 301 NSFESQQFGRKSLDVNYPPEFSSVFSEGDRLSTSSQSMEEVEAEMRRLKLELKQTMEMYS 360
           +SFESQ FGRKS+DVN+  EFSS+FSEG+RLSTSSQSMEEVEAEMRRLKLELKQTMEMYS
Sbjct: 301 HSFESQPFGRKSMDVNF-QEFSSIFSEGERLSTSSQSMEEVEAEMRRLKLELKQTMEMYS 360

Query: 361 TACKEALTAKQKAIELQRWKSEEERRIEEARLAEEAALEMVEQEKAKSRAAIEAAEAAKR 420
           TACKEALTAKQKAIELQRWKSEEERRIEEARLAEEAAL MVEQEKAKSR+A+EAAEA+KR
Sbjct: 361 TACKEALTAKQKAIELQRWKSEEERRIEEARLAEEAALAMVEQEKAKSRSAMEAAEASKR 420

Query: 421 IAELEAQKRINAEMKAFKEAEEKKKALDALAHNDVRYRKYSIEEIEAATEFFSESLKIGE 480
           IAELEAQKRINAE+KAFKEAEEKKKALDALA+NDVRYR+Y+IEEIEAATEFFSESLKIGE
Sbjct: 421 IAELEAQKRINAEIKAFKEAEEKKKALDALANNDVRYRRYTIEEIEAATEFFSESLKIGE 480

Query: 481 GGYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGCL 540
           GGYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGCL
Sbjct: 481 GGYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGCL 540

Query: 541 VYEFMANGSLDDCLFRRKENSKPLSWQLRFKIAAEIGTSLLFLHQAKPEPLVHRDLKPAN 600
           VYEFMANGSLDDCLFRRKE SKPLSWQLRFKIAAEIGTSLLFLHQ KPEPLVHRDLKPAN
Sbjct: 541 VYEFMANGSLDDCLFRRKEKSKPLSWQLRFKIAAEIGTSLLFLHQTKPEPLVHRDLKPAN 600

Query: 601 ILLDRNYVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIY 660
           ILLD NYVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIY
Sbjct: 601 ILLDFNYVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIY 660

Query: 661 SLGIMFLQLITAKPPMGLTHHVGRAIEKGTLLEFLDPSVTDWPAEESLCFAELALKCAEL 720
           SLGIMFLQLITAKPPMGLTHHVGRAIEKG L E LDPSV DWPAEE+LCFAELALKCAEL
Sbjct: 661 SLGIMFLQLITAKPPMGLTHHVGRAIEKGDLSEVLDPSVLDWPAEETLCFAELALKCAEL 720

Query: 721 RRKDRPDLRKVVLPELIRLRTIAEASMYSSVTDGV 751
           RRKDRPDL K VLPELIRLR++AEAS+YSS  DGV
Sbjct: 721 RRKDRPDLSKTVLPELIRLRSVAEASLYSSDKDGV 749

BLAST of Lcy01g011020 vs. ExPASy TrEMBL
Match: A0A6J1KND3 (U-box domain-containing protein 52-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496144 PE=4 SV=1)

HSP 1 Score: 1273.1 bits (3293), Expect = 0.0e+00
Identity = 669/756 (88.49%), Postives = 706/756 (93.39%), Query Frame = 0

Query: 1   MWNQKPVKEKKE-VVTGNELVGIAIDKDKGSQNALKWATENLLIKGQTAILIHVKLRS-- 60
           MW QK  KE+KE VV+GN+LVGIAIDKDKGSQNALKWATENLL KGQTAIL+HVKLRS  
Sbjct: 73  MWTQKYAKERKEVVVSGNQLVGIAIDKDKGSQNALKWATENLLQKGQTAILVHVKLRSSS 132

Query: 61  -SGSSLTSPKVMSATDNGNDNVNVLVSKDIDPNTRDLFLPFRCFCTRKDIFCKDIVIEDV 120
            SGSS  S  +MS   +G    NVLVS +IDP+TRDLFLPFRCFCTRKDIFCKDIVIEDV
Sbjct: 133 HSGSSYASSDLMSNNGSG----NVLVSNEIDPSTRDLFLPFRCFCTRKDIFCKDIVIEDV 192

Query: 121 DVAKALIEYVSQAGIETLVVGTTTKGGFLRRFKTTDIPGSVIKGAPDFCNVYVISRGKIQ 180
           DVAKALIEYV+QAGI+TL+VGTTTK GFL RFKTTDIPG+++KGAPDFCNVYVISRGKIQ
Sbjct: 193 DVAKALIEYVTQAGIDTLIVGTTTKSGFL-RFKTTDIPGTILKGAPDFCNVYVISRGKIQ 252

Query: 181 TMRSASRPAPTSSPLRSLLLSQSGSRSQLSESQISTIQ-RPEKPPIEPARRSQDNIEFRS 240
           TMRSASRPAPTSSPLR+ LLSQSG +SQ S+SQ   IQ  PEKPPIEPARRS +NIEFRS
Sbjct: 253 TMRSASRPAPTSSPLRNFLLSQSGIKSQSSDSQTPQIQIGPEKPPIEPARRSHENIEFRS 312

Query: 241 PFTRKGYNGKMPGEMNLPDTDISFVSSGRPSVDKLFPTFFDA-DSGRIPPRLSNGTDGDI 300
           PFTRKGYNGKMPGEM++PDTDISFV SGRPSVDKLFPT++DA D GR+PPRLSNGTDGDI
Sbjct: 313 PFTRKGYNGKMPGEMSVPDTDISFVGSGRPSVDKLFPTYYDAVDPGRMPPRLSNGTDGDI 372

Query: 301 YNSFESQQFGRKSLDVNYPPEFSSVFSEGDRLSTSSQSMEEVEAEMRRLKLELKQTMEMY 360
           Y+SFESQ FGRKSLDVN+  EFSS+FSEG+RLSTSSQSMEEVEAEMRRLKLELKQTMEMY
Sbjct: 373 YHSFESQPFGRKSLDVNF-QEFSSIFSEGERLSTSSQSMEEVEAEMRRLKLELKQTMEMY 432

Query: 361 STACKEALTAKQKAIELQRWKSEEERRIEEARLAEEAALEMVEQEKAKSRAAIEAAEAAK 420
           STACKEALTAKQKAIELQRWKSEEERRIEEARLAEEAAL MVEQEKAKSRAA+EAAEA+K
Sbjct: 433 STACKEALTAKQKAIELQRWKSEEERRIEEARLAEEAALAMVEQEKAKSRAAMEAAEASK 492

Query: 421 RIAELEAQKRINAEMKAFKEAEEKKKALDALAHNDVRYRKYSIEEIEAATEFFSESLKIG 480
           RIAELEAQKRINAE+KAFKEAEEKKKALDALA+NDVRYR+Y+IEEIEAATEFFSESLKIG
Sbjct: 493 RIAELEAQKRINAEIKAFKEAEEKKKALDALANNDVRYRRYTIEEIEAATEFFSESLKIG 552

Query: 481 EGGYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGC 540
           EGGYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGC
Sbjct: 553 EGGYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGC 612

Query: 541 LVYEFMANGSLDDCLFRRKENSKPLSWQLRFKIAAEIGTSLLFLHQAKPEPLVHRDLKPA 600
           LVYEFMANGSLDDCLFRRKE SKPLSWQLRFKIAAEIGTSLLFLHQ KPEPLVHRDLKPA
Sbjct: 613 LVYEFMANGSLDDCLFRRKEKSKPLSWQLRFKIAAEIGTSLLFLHQTKPEPLVHRDLKPA 672

Query: 601 NILLDRNYVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDI 660
           NILLD NYVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDI
Sbjct: 673 NILLDFNYVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDI 732

Query: 661 YSLGIMFLQLITAKPPMGLTHHVGRAIEKGTLLEFLDPSVTDWPAEESLCFAELALKCAE 720
           YSLGIMFLQLITAKPPMGLTHHVGRAIEKG L E LDPSV DWPAEE+LCFAELALKCAE
Sbjct: 733 YSLGIMFLQLITAKPPMGLTHHVGRAIEKGNLSEVLDPSVLDWPAEETLCFAELALKCAE 792

Query: 721 LRRKDRPDLRKVVLPELIRLRTIAEASMYSSVTDGV 751
           LRRKDRPDL   VLPELIRLR++AEAS+YSS  DGV
Sbjct: 793 LRRKDRPDLSMTVLPELIRLRSVAEASLYSSDKDGV 822

BLAST of Lcy01g011020 vs. NCBI nr
Match: XP_038886007.1 (U-box domain-containing protein 35-like isoform X1 [Benincasa hispida] >XP_038886008.1 U-box domain-containing protein 35-like isoform X2 [Benincasa hispida])

HSP 1 Score: 1332.8 bits (3448), Expect = 0.0e+00
Identity = 692/794 (87.15%), Postives = 742/794 (93.45%), Query Frame = 0

Query: 1   MWNQKPV--KEKKEVVTGNELVGIAIDKDKGSQNALKWATENLLIKGQTAILIHVKLRSS 60
           MWNQK    KE+KE+ T N+LVGIAIDKDKGSQ ALKWATENLL KGQTAILIHVKL+SS
Sbjct: 1   MWNQKQSIGKERKELSTANQLVGIAIDKDKGSQVALKWATENLLGKGQTAILIHVKLKSS 60

Query: 61  ---GSSLTSPKVMSATDNGNDNVNVLVSKDIDPNTRDLFLPFRCFCTRKDIFCKDIVIED 120
              GSS+ SPKVMSA+D GN N NV+VSKD+DP+TRDLFLPFRCFCTRKDIFCKDI++ED
Sbjct: 61  SYPGSSMASPKVMSASDTGNGNGNVMVSKDVDPSTRDLFLPFRCFCTRKDIFCKDIIVED 120

Query: 121 VDVAKALIEYVSQAGIETLVVGTTTKGGFLRRFKTTDIPGSVIKGAPDFCNVYVISRGKI 180
           +DVAKALIEYV+QAGIE LVVG+T+K GFLRRFKTTDIPGSV+KGAPDFCNVYVISRGKI
Sbjct: 121 LDVAKALIEYVTQAGIENLVVGSTSKSGFLRRFKTTDIPGSVVKGAPDFCNVYVISRGKI 180

Query: 181 QTMRSASRPAPTSSPLRSLLLSQSGSRSQLSESQISTIQRPEKPPIEPARRSQDNIEFRS 240
           QTMRSASRPAP SSPLR+LLLSQ+G +S  +ES +  IQRPEKPP+E ARRSQDNIEFRS
Sbjct: 181 QTMRSASRPAPASSPLRNLLLSQAGIKSNSAESPVPQIQRPEKPPLEAARRSQDNIEFRS 240

Query: 241 PFTRKGYNGKMPGEMNLPDTDISFVSSGRPSVDKLFPTFFD--ADSGRIPPRLSNGTDGD 300
           PFTRKGYNGKMPGE+++PD+DISFVSSGRPSVD+LFPT FD   D+GR+PPRLS+GTD D
Sbjct: 241 PFTRKGYNGKMPGEVSVPDSDISFVSSGRPSVDRLFPTLFDNNLDTGRVPPRLSSGTDAD 300

Query: 301 IYNSFESQQFGRKSLDVNYPPEFSSVFSEGDRLSTSSQSMEEVEAEMRRLKLELKQTMEM 360
           IYNSFESQ FGRKSLDVNYPPEF SVFSEGDRLSTSSQSMEEVEAEMRRLKLELKQTMEM
Sbjct: 301 IYNSFESQPFGRKSLDVNYPPEFLSVFSEGDRLSTSSQSMEEVEAEMRRLKLELKQTMEM 360

Query: 361 YSTACKEALTAKQKAIELQRWKSEEERRIEEARLAEEAALEMVEQEKAKSRAAIEAAEAA 420
           YSTACKEALTAKQKAIELQRWKSEEERRIEEARLAEEAAL MVEQEKAKSRAAIEAAEAA
Sbjct: 361 YSTACKEALTAKQKAIELQRWKSEEERRIEEARLAEEAALAMVEQEKAKSRAAIEAAEAA 420

Query: 421 KRIAELEAQKRINAEMKAFKEAEEKKKALDALAHNDVRYRKYSIEEIEAATEFFSESLKI 480
           KRIAELEAQKRINAE KAFKEAEEKKKALDALA+NDVRYRKYSI+EIE ATEFFSESLKI
Sbjct: 421 KRIAELEAQKRINAEKKAFKEAEEKKKALDALANNDVRYRKYSIDEIETATEFFSESLKI 480

Query: 481 GEGGYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYG 540
           GEGGYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYG
Sbjct: 481 GEGGYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYG 540

Query: 541 CLVYEFMANGSLDDCLFRRKENSKPLSWQLRFKIAAEIGTSLLFLHQAKPEPLVHRDLKP 600
           CLVYEFMA+GSLDDCLFRR+ENSKPLSWQ RFKIAAEIGT+LLFLHQ KPEPLVHRDLKP
Sbjct: 541 CLVYEFMAHGSLDDCLFRRRENSKPLSWQRRFKIAAEIGTALLFLHQTKPEPLVHRDLKP 600

Query: 601 ANILLDRNYVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSD 660
           ANILLDRNYVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSD
Sbjct: 601 ANILLDRNYVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSD 660

Query: 661 IYSLGIMFLQLITAKPPMGLTHHVGRAIEKGTLLEFLDPSVTDWPAEESLCFAELALKCA 720
           +YSLGIMFLQLITA+ PMGLTHHVGRAIEKGTL E LDPSVTDWPAEE+LCFAELALKCA
Sbjct: 661 VYSLGIMFLQLITARAPMGLTHHVGRAIEKGTLSEILDPSVTDWPAEETLCFAELALKCA 720

Query: 721 ELRRKDRPDLRKVVLPELIRLRTIAEASMYSSVTDGVSSPEFSNSQIRLKTDVSGESFSS 780
           ELRRKDRPDL KVVLPELIR +++AEASMY+ VTDGVSSP FS++Q+ ++ D  GE FSS
Sbjct: 721 ELRRKDRPDLGKVVLPELIRFQSLAEASMYAPVTDGVSSPVFSHNQVSMRRD--GECFSS 780

Query: 781 RSCNSPGVLSHSLS 788
           +S +S GVLS S+S
Sbjct: 781 QSGDSLGVLSRSVS 792

BLAST of Lcy01g011020 vs. NCBI nr
Match: XP_038886009.1 (U-box domain-containing protein 35-like isoform X3 [Benincasa hispida])

HSP 1 Score: 1325.8 bits (3430), Expect = 0.0e+00
Identity = 691/794 (87.03%), Postives = 741/794 (93.32%), Query Frame = 0

Query: 1   MWNQKPV--KEKKEVVTGNELVGIAIDKDKGSQNALKWATENLLIKGQTAILIHVKLRSS 60
           MWNQK    KE+KE+ T N+LVGIAIDKDKGSQ ALKWATENLL KGQTAILIHVKL+SS
Sbjct: 1   MWNQKQSIGKERKELSTANQLVGIAIDKDKGSQVALKWATENLLGKGQTAILIHVKLKSS 60

Query: 61  ---GSSLTSPKVMSATDNGNDNVNVLVSKDIDPNTRDLFLPFRCFCTRKDIFCKDIVIED 120
              GSS+ SPKVMSA+D GN N NV+VSKD+DP+TRDLFLPFRCFCTRKDIFCKDI++ED
Sbjct: 61  SYPGSSMASPKVMSASDTGNGNGNVMVSKDVDPSTRDLFLPFRCFCTRKDIFCKDIIVED 120

Query: 121 VDVAKALIEYVSQAGIETLVVGTTTKGGFLRRFKTTDIPGSVIKGAPDFCNVYVISRGKI 180
           +DVAKALIEYV+QAGIE LVVG+T+K GFL RFKTTDIPGSV+KGAPDFCNVYVISRGKI
Sbjct: 121 LDVAKALIEYVTQAGIENLVVGSTSKSGFL-RFKTTDIPGSVVKGAPDFCNVYVISRGKI 180

Query: 181 QTMRSASRPAPTSSPLRSLLLSQSGSRSQLSESQISTIQRPEKPPIEPARRSQDNIEFRS 240
           QTMRSASRPAP SSPLR+LLLSQ+G +S  +ES +  IQRPEKPP+E ARRSQDNIEFRS
Sbjct: 181 QTMRSASRPAPASSPLRNLLLSQAGIKSNSAESPVPQIQRPEKPPLEAARRSQDNIEFRS 240

Query: 241 PFTRKGYNGKMPGEMNLPDTDISFVSSGRPSVDKLFPTFFD--ADSGRIPPRLSNGTDGD 300
           PFTRKGYNGKMPGE+++PD+DISFVSSGRPSVD+LFPT FD   D+GR+PPRLS+GTD D
Sbjct: 241 PFTRKGYNGKMPGEVSVPDSDISFVSSGRPSVDRLFPTLFDNNLDTGRVPPRLSSGTDAD 300

Query: 301 IYNSFESQQFGRKSLDVNYPPEFSSVFSEGDRLSTSSQSMEEVEAEMRRLKLELKQTMEM 360
           IYNSFESQ FGRKSLDVNYPPEF SVFSEGDRLSTSSQSMEEVEAEMRRLKLELKQTMEM
Sbjct: 301 IYNSFESQPFGRKSLDVNYPPEFLSVFSEGDRLSTSSQSMEEVEAEMRRLKLELKQTMEM 360

Query: 361 YSTACKEALTAKQKAIELQRWKSEEERRIEEARLAEEAALEMVEQEKAKSRAAIEAAEAA 420
           YSTACKEALTAKQKAIELQRWKSEEERRIEEARLAEEAAL MVEQEKAKSRAAIEAAEAA
Sbjct: 361 YSTACKEALTAKQKAIELQRWKSEEERRIEEARLAEEAALAMVEQEKAKSRAAIEAAEAA 420

Query: 421 KRIAELEAQKRINAEMKAFKEAEEKKKALDALAHNDVRYRKYSIEEIEAATEFFSESLKI 480
           KRIAELEAQKRINAE KAFKEAEEKKKALDALA+NDVRYRKYSI+EIE ATEFFSESLKI
Sbjct: 421 KRIAELEAQKRINAEKKAFKEAEEKKKALDALANNDVRYRKYSIDEIETATEFFSESLKI 480

Query: 481 GEGGYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYG 540
           GEGGYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYG
Sbjct: 481 GEGGYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYG 540

Query: 541 CLVYEFMANGSLDDCLFRRKENSKPLSWQLRFKIAAEIGTSLLFLHQAKPEPLVHRDLKP 600
           CLVYEFMA+GSLDDCLFRR+ENSKPLSWQ RFKIAAEIGT+LLFLHQ KPEPLVHRDLKP
Sbjct: 541 CLVYEFMAHGSLDDCLFRRRENSKPLSWQRRFKIAAEIGTALLFLHQTKPEPLVHRDLKP 600

Query: 601 ANILLDRNYVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSD 660
           ANILLDRNYVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSD
Sbjct: 601 ANILLDRNYVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSD 660

Query: 661 IYSLGIMFLQLITAKPPMGLTHHVGRAIEKGTLLEFLDPSVTDWPAEESLCFAELALKCA 720
           +YSLGIMFLQLITA+ PMGLTHHVGRAIEKGTL E LDPSVTDWPAEE+LCFAELALKCA
Sbjct: 661 VYSLGIMFLQLITARAPMGLTHHVGRAIEKGTLSEILDPSVTDWPAEETLCFAELALKCA 720

Query: 721 ELRRKDRPDLRKVVLPELIRLRTIAEASMYSSVTDGVSSPEFSNSQIRLKTDVSGESFSS 780
           ELRRKDRPDL KVVLPELIR +++AEASMY+ VTDGVSSP FS++Q+ ++ D  GE FSS
Sbjct: 721 ELRRKDRPDLGKVVLPELIRFQSLAEASMYAPVTDGVSSPVFSHNQVSMRRD--GECFSS 780

Query: 781 RSCNSPGVLSHSLS 788
           +S +S GVLS S+S
Sbjct: 781 QSGDSLGVLSRSVS 791

BLAST of Lcy01g011020 vs. NCBI nr
Match: XP_038886010.1 (U-box domain-containing protein 35-like isoform X4 [Benincasa hispida])

HSP 1 Score: 1325.8 bits (3430), Expect = 0.0e+00
Identity = 691/794 (87.03%), Postives = 738/794 (92.95%), Query Frame = 0

Query: 1   MWNQKPV--KEKKEVVTGNELVGIAIDKDKGSQNALKWATENLLIKGQTAILIHVKLRSS 60
           MWNQK    KE+KE+ T N+LVGIAIDKDKGSQ ALKWATENLL KGQTAILIHVKL+SS
Sbjct: 1   MWNQKQSIGKERKELSTANQLVGIAIDKDKGSQVALKWATENLLGKGQTAILIHVKLKSS 60

Query: 61  ---GSSLTSPKVMSATDNGNDNVNVLVSKDIDPNTRDLFLPFRCFCTRKDIFCKDIVIED 120
              GSS+ SPKVMSA+D GN N NV+VSKD+DP+TRDLFLPFRCFCTRKDIFCKDI++ED
Sbjct: 61  SYPGSSMASPKVMSASDTGNGNGNVMVSKDVDPSTRDLFLPFRCFCTRKDIFCKDIIVED 120

Query: 121 VDVAKALIEYVSQAGIETLVVGTTTKGGFLRRFKTTDIPGSVIKGAPDFCNVYVISRGKI 180
           +DVAKALIEYV+QAGIE LVVG+T+K GFLRRFKTTDIPGSV+KGAPDFCNVYVISRGKI
Sbjct: 121 LDVAKALIEYVTQAGIENLVVGSTSKSGFLRRFKTTDIPGSVVKGAPDFCNVYVISRGKI 180

Query: 181 QTMRSASRPAPTSSPLRSLLLSQSGSRSQLSESQISTIQRPEKPPIEPARRSQDNIEFRS 240
           QTMRSASRPAP SSPLR+LLLSQ+G +S  +ES +  IQRPEKPP+E ARRSQDNIEFRS
Sbjct: 181 QTMRSASRPAPASSPLRNLLLSQAGIKSNSAESPVPQIQRPEKPPLEAARRSQDNIEFRS 240

Query: 241 PFTRKGYNGKMPGEMNLPDTDISFVSSGRPSVDKLFPTFFD--ADSGRIPPRLSNGTDGD 300
           PFTRKGYNGKMPGE+++PD+DISFVSSGRPSVD+LFPT FD   D+GR+PPRLS+GTD D
Sbjct: 241 PFTRKGYNGKMPGEVSVPDSDISFVSSGRPSVDRLFPTLFDNNLDTGRVPPRLSSGTDAD 300

Query: 301 IYNSFESQQFGRKSLDVNYPPEFSSVFSEGDRLSTSSQSMEEVEAEMRRLKLELKQTMEM 360
           IYNSFESQ FGRKSLDVNYPPEF SVFSEGDRLSTSSQSMEEVEAEMRRLKLELKQTMEM
Sbjct: 301 IYNSFESQPFGRKSLDVNYPPEFLSVFSEGDRLSTSSQSMEEVEAEMRRLKLELKQTMEM 360

Query: 361 YSTACKEALTAKQKAIELQRWKSEEERRIEEARLAEEAALEMVEQEKAKSRAAIEAAEAA 420
           YSTACKEALTAKQKAIELQRWKSEEERRIEEARLAEEAAL MVEQEKAKSRAAIEAAEAA
Sbjct: 361 YSTACKEALTAKQKAIELQRWKSEEERRIEEARLAEEAALAMVEQEKAKSRAAIEAAEAA 420

Query: 421 KRIAELEAQKRINAEMKAFKEAEEKKKALDALAHNDVRYRKYSIEEIEAATEFFSESLKI 480
           KRIAELEAQKRINAE KAFKEAEEKKKALDALA+NDVRYRKYSI+EIE ATEFFSESLKI
Sbjct: 421 KRIAELEAQKRINAEKKAFKEAEEKKKALDALANNDVRYRKYSIDEIETATEFFSESLKI 480

Query: 481 GEGGYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYG 540
           GEGGYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYG
Sbjct: 481 GEGGYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYG 540

Query: 541 CLVYEFMANGSLDDCLFRRKENSKPLSWQLRFKIAAEIGTSLLFLHQAKPEPLVHRDLKP 600
           CLVYEFMA+GSLDDCLFRR+ENSKPLSWQ RFKIAAEIGT+LLFLHQ KPEPLVHRDLKP
Sbjct: 541 CLVYEFMAHGSLDDCLFRRRENSKPLSWQRRFKIAAEIGTALLFLHQTKPEPLVHRDLKP 600

Query: 601 ANILLDRNYVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSD 660
           ANILLDRNYVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSD
Sbjct: 601 ANILLDRNYVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSD 660

Query: 661 IYSLGIMFLQLITAKPPMGLTHHVGRAIEKGTLLEFLDPSVTDWPAEESLCFAELALKCA 720
           +YSLGIMFLQLITA+ PMGLTHHVGRAIEKGTL E LDPSVTDWPAEE+LCFAELALKCA
Sbjct: 661 VYSLGIMFLQLITARAPMGLTHHVGRAIEKGTLSEILDPSVTDWPAEETLCFAELALKCA 720

Query: 721 ELRRKDRPDLRKVVLPELIRLRTIAEASMYSSVTDGVSSPEFSNSQIRLKTDVSGESFSS 780
           ELRRKDRPDL KVVLPELIR +++AEASMY+ VTDGVSSP FS++Q        GE FSS
Sbjct: 721 ELRRKDRPDLGKVVLPELIRFQSLAEASMYAPVTDGVSSPVFSHNQ-------DGECFSS 780

Query: 781 RSCNSPGVLSHSLS 788
           +S +S GVLS S+S
Sbjct: 781 QSGDSLGVLSRSVS 787

BLAST of Lcy01g011020 vs. NCBI nr
Match: XP_022131588.1 (U-box domain-containing protein 35-like isoform X1 [Momordica charantia])

HSP 1 Score: 1297.7 bits (3357), Expect = 0.0e+00
Identity = 675/783 (86.21%), Postives = 720/783 (91.95%), Query Frame = 0

Query: 1   MWNQKPVKEKKEVVTGNELVGIAIDKDKGSQNALKWATENLLIKGQTAILIHVKLRSSGS 60
           MWNQK  KE KE  TGNELV IAIDKDKGSQNALKWATENLL+KGQTAILIHVKL+SS +
Sbjct: 1   MWNQKQTKE-KEGATGNELVAIAIDKDKGSQNALKWATENLLLKGQTAILIHVKLKSSSN 60

Query: 61  S---LTSPKVMSATDNGNDNVNVLVSKDIDPNTRDLFLPFRCFCTRKDIFCKDIVIEDVD 120
           S   + SPKV SATDN N   N L+ KD+DP TRDLFLPFRCFCTRKDIFCKDI++ED D
Sbjct: 61  STSYMASPKVTSATDNANG--NFLIGKDLDPVTRDLFLPFRCFCTRKDIFCKDIILEDTD 120

Query: 121 VAKALIEYVSQAGIETLVVGTTTKGGFLRRFKTTDIPGSVIKGAPDFCNVYVISRGKIQT 180
           V+KAL+EYVSQA IE LVVGTTTK GFLRRFKTTDIPGSVIKGAPDFC VYVISRGKIQT
Sbjct: 121 VSKALLEYVSQAAIENLVVGTTTKNGFLRRFKTTDIPGSVIKGAPDFCTVYVISRGKIQT 180

Query: 181 MRSASRPAPTSSPLRSLLLSQSGSRSQLSESQISTIQRPEKPPIEPARRSQDNIEFRSPF 240
           MRSASRPAPTSSPLRS LL+QSG +S LSESQ+S IQR EKPP+E ARRSQDNIEFRSPF
Sbjct: 181 MRSASRPAPTSSPLRSFLLNQSGIKSPLSESQVSQIQRIEKPPLEAARRSQDNIEFRSPF 240

Query: 241 TRKGYNGKMPGEMNLPDTDISFVSSGRPSVDKLFPTFFDA-DSGRIPPRLSNGTDGDIYN 300
           TRKGYNGKMPGEM+LPDTDISFVSSGRPSVD+LFP++FD  DSGR+ PRLS+GT+ D+YN
Sbjct: 241 TRKGYNGKMPGEMSLPDTDISFVSSGRPSVDRLFPSYFDTPDSGRVTPRLSSGTEADMYN 300

Query: 301 SFESQQFGRKSLDVNYPPEFSSVFSEGDRLSTSSQSMEEVEAEMRRLKLELKQTMEMYST 360
           SFESQ FGR+S+DVNYPPEF SVF +GDRLSTSSQ+ME+VEAEMRRLKLELKQTMEMYST
Sbjct: 301 SFESQPFGRRSVDVNYPPEFLSVFPDGDRLSTSSQNMEDVEAEMRRLKLELKQTMEMYST 360

Query: 361 ACKEALTAKQKAIELQRWKSEEERRIEEARLAEEAALEMVEQEKAKSRAAIEAAEAAKRI 420
           ACKEALTAKQKA+ELQRWKSEEERR+EEARLAEEAAL +VEQEKAKS+AAIEAAEAAKRI
Sbjct: 361 ACKEALTAKQKAVELQRWKSEEERRLEEARLAEEAALAIVEQEKAKSQAAIEAAEAAKRI 420

Query: 421 AELEAQKRINAEMKAFKEAEEKKKALDALAHNDVRYRKYSIEEIEAATEFFSESLKIGEG 480
           AELEAQKRINAE+KAFKEAEEKKKALDALAH DVRYRKYSIEEIEAATEFFSESLKIGEG
Sbjct: 421 AELEAQKRINAELKAFKEAEEKKKALDALAHTDVRYRKYSIEEIEAATEFFSESLKIGEG 480

Query: 481 GYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGCLV 540
           GYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGCLV
Sbjct: 481 GYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGCLV 540

Query: 541 YEFMANGSLDDCLFRRKENSKPLSWQLRFKIAAEIGTSLLFLHQAKPEPLVHRDLKPANI 600
           YEFMANGSLDDCLF R+EN KPLSWQLRFKIAAEIGTSLLFLHQ KPEPLVHRDLKPANI
Sbjct: 541 YEFMANGSLDDCLFHRRENLKPLSWQLRFKIAAEIGTSLLFLHQTKPEPLVHRDLKPANI 600

Query: 601 LLDRNYVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYS 660
           LLDRN+VSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYS
Sbjct: 601 LLDRNFVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYS 660

Query: 661 LGIMFLQLITAKPPMGLTHHVGRAIEKGTLLEFLDPSVTDWPAEESLCFAELALKCAELR 720
           LGIMFLQLITAKPPMGLTHHV RAIEKG   E LD SVTDWPAEE+LCFA+LALKCAELR
Sbjct: 661 LGIMFLQLITAKPPMGLTHHVERAIEKGKFSELLDQSVTDWPAEETLCFAQLALKCAELR 720

Query: 721 RKDRPDLRKVVLPELIRLRTIAEASMYSSVTDGVSSPEFSNSQI-RLKTDVSGESFSSRS 779
           RKDRPDLRKVVLPELIR R++AEA+    + DGV+SP FS SQ+   K D+SG+  SS+S
Sbjct: 721 RKDRPDLRKVVLPELIRFRSLAEANTSPPMADGVTSPVFSRSQVSAFKQDLSGDRVSSQS 780

BLAST of Lcy01g011020 vs. NCBI nr
Match: XP_022131589.1 (U-box domain-containing protein 35-like isoform X2 [Momordica charantia])

HSP 1 Score: 1290.8 bits (3339), Expect = 0.0e+00
Identity = 674/783 (86.08%), Postives = 719/783 (91.83%), Query Frame = 0

Query: 1   MWNQKPVKEKKEVVTGNELVGIAIDKDKGSQNALKWATENLLIKGQTAILIHVKLRSSGS 60
           MWNQK  KE KE  TGNELV IAIDKDKGSQNALKWATENLL+KGQTAILIHVKL+SS +
Sbjct: 1   MWNQKQTKE-KEGATGNELVAIAIDKDKGSQNALKWATENLLLKGQTAILIHVKLKSSSN 60

Query: 61  S---LTSPKVMSATDNGNDNVNVLVSKDIDPNTRDLFLPFRCFCTRKDIFCKDIVIEDVD 120
           S   + SPKV SATDN N   N L+ KD+DP TRDLFLPFRCFCTRKDIFCKDI++ED D
Sbjct: 61  STSYMASPKVTSATDNANG--NFLIGKDLDPVTRDLFLPFRCFCTRKDIFCKDIILEDTD 120

Query: 121 VAKALIEYVSQAGIETLVVGTTTKGGFLRRFKTTDIPGSVIKGAPDFCNVYVISRGKIQT 180
           V+KAL+EYVSQA IE LVVGTTTK GFL RFKTTDIPGSVIKGAPDFC VYVISRGKIQT
Sbjct: 121 VSKALLEYVSQAAIENLVVGTTTKNGFL-RFKTTDIPGSVIKGAPDFCTVYVISRGKIQT 180

Query: 181 MRSASRPAPTSSPLRSLLLSQSGSRSQLSESQISTIQRPEKPPIEPARRSQDNIEFRSPF 240
           MRSASRPAPTSSPLRS LL+QSG +S LSESQ+S IQR EKPP+E ARRSQDNIEFRSPF
Sbjct: 181 MRSASRPAPTSSPLRSFLLNQSGIKSPLSESQVSQIQRIEKPPLEAARRSQDNIEFRSPF 240

Query: 241 TRKGYNGKMPGEMNLPDTDISFVSSGRPSVDKLFPTFFDA-DSGRIPPRLSNGTDGDIYN 300
           TRKGYNGKMPGEM+LPDTDISFVSSGRPSVD+LFP++FD  DSGR+ PRLS+GT+ D+YN
Sbjct: 241 TRKGYNGKMPGEMSLPDTDISFVSSGRPSVDRLFPSYFDTPDSGRVTPRLSSGTEADMYN 300

Query: 301 SFESQQFGRKSLDVNYPPEFSSVFSEGDRLSTSSQSMEEVEAEMRRLKLELKQTMEMYST 360
           SFESQ FGR+S+DVNYPPEF SVF +GDRLSTSSQ+ME+VEAEMRRLKLELKQTMEMYST
Sbjct: 301 SFESQPFGRRSVDVNYPPEFLSVFPDGDRLSTSSQNMEDVEAEMRRLKLELKQTMEMYST 360

Query: 361 ACKEALTAKQKAIELQRWKSEEERRIEEARLAEEAALEMVEQEKAKSRAAIEAAEAAKRI 420
           ACKEALTAKQKA+ELQRWKSEEERR+EEARLAEEAAL +VEQEKAKS+AAIEAAEAAKRI
Sbjct: 361 ACKEALTAKQKAVELQRWKSEEERRLEEARLAEEAALAIVEQEKAKSQAAIEAAEAAKRI 420

Query: 421 AELEAQKRINAEMKAFKEAEEKKKALDALAHNDVRYRKYSIEEIEAATEFFSESLKIGEG 480
           AELEAQKRINAE+KAFKEAEEKKKALDALAH DVRYRKYSIEEIEAATEFFSESLKIGEG
Sbjct: 421 AELEAQKRINAELKAFKEAEEKKKALDALAHTDVRYRKYSIEEIEAATEFFSESLKIGEG 480

Query: 481 GYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGCLV 540
           GYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGCLV
Sbjct: 481 GYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGCLV 540

Query: 541 YEFMANGSLDDCLFRRKENSKPLSWQLRFKIAAEIGTSLLFLHQAKPEPLVHRDLKPANI 600
           YEFMANGSLDDCLF R+EN KPLSWQLRFKIAAEIGTSLLFLHQ KPEPLVHRDLKPANI
Sbjct: 541 YEFMANGSLDDCLFHRRENLKPLSWQLRFKIAAEIGTSLLFLHQTKPEPLVHRDLKPANI 600

Query: 601 LLDRNYVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYS 660
           LLDRN+VSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYS
Sbjct: 601 LLDRNFVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYS 660

Query: 661 LGIMFLQLITAKPPMGLTHHVGRAIEKGTLLEFLDPSVTDWPAEESLCFAELALKCAELR 720
           LGIMFLQLITAKPPMGLTHHV RAIEKG   E LD SVTDWPAEE+LCFA+LALKCAELR
Sbjct: 661 LGIMFLQLITAKPPMGLTHHVERAIEKGKFSELLDQSVTDWPAEETLCFAQLALKCAELR 720

Query: 721 RKDRPDLRKVVLPELIRLRTIAEASMYSSVTDGVSSPEFSNSQI-RLKTDVSGESFSSRS 779
           RKDRPDLRKVVLPELIR R++AEA+    + DGV+SP FS SQ+   K D+SG+  SS+S
Sbjct: 721 RKDRPDLRKVVLPELIRFRSLAEANTSPPMADGVTSPVFSRSQVSAFKQDLSGDRVSSQS 779

BLAST of Lcy01g011020 vs. TAIR 10
Match: AT2G24370.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 959.9 bits (2480), Expect = 1.3e-279
Identity = 517/776 (66.62%), Postives = 622/776 (80.15%), Query Frame = 0

Query: 19  LVGIAIDKDKGSQNALKWATENLLIKGQTAILIHVKLRSSGSSLTSPKVMSATDNGNDNV 78
           +V +AIDKDK SQ+ALKWA +NLL +GQ+ IL+HVKLR   S L +   + A+       
Sbjct: 19  VVAVAIDKDKSSQHALKWAVDNLLQRGQSVILVHVKLRP--SPLNNSASLHASSAKLSQD 78

Query: 79  NVLVSKDIDPNTRDLFLPFRCFCTRKDIFCKDIVIEDVDVAKALIEYVSQAGIETLVVGT 138
           + LV +D +  ++++FLPFRCFCTRKDI C+D+++E+ DVAKAL+EYV+QA IE LVVG+
Sbjct: 79  SSLVCRDPEGISKEIFLPFRCFCTRKDIQCQDVLLEESDVAKALVEYVNQAAIEVLVVGS 138

Query: 139 TTKGGFLRRFKTTDIPGSVIKGAPDFCNVYVISRGKIQTMRSASRPAPTSSPLRSLLLSQ 198
           ++KGGFLR  K TDIPGS+ K APDFC VY+IS+GKIQTMRSASR AP ++PLRS +   
Sbjct: 139 SSKGGFLRFNKPTDIPGSITKNAPDFCTVYIISKGKIQTMRSASRSAPMTAPLRSSVQPP 198

Query: 199 S----------------GSRSQLSESQISTIQRPEKPPIEPARR---SQDNIEFRSPFTR 258
           S                  R     +Q  +++  ++  +E  +R      +  FRSPFTR
Sbjct: 199 SLKPPQPMPSTSANSLRADRRSFESNQRRSVEDQQRRSMEDQQRRSIDDQSESFRSPFTR 258

Query: 259 KGYNGKMPGEMNLPDTDISFVSSGRPSVDKLFPTFFD-ADSGRIPPRLSNGTDGDIYNSF 318
           +G NG+  G++ +P++DISF+SSGRPS+D++FP+ +D  D  R PPRLSN +D D  +S 
Sbjct: 259 RG-NGRSYGDLTVPESDISFISSGRPSIDRIFPSLYDNNDPSRTPPRLSNFSDMDYSSSL 318

Query: 319 -ESQQFGRKSLDVNYPPEFSSVFSEGDRLSTSSQSMEEVEAEMRRLKLELKQTMEMYSTA 378
            +S  +GR+S+D+N P +F     E +R S++SQS+++VEAEMRRLKLELKQTMEMYSTA
Sbjct: 319 DQSSNYGRRSVDMNSPTDF-----ESERFSSASQSIDDVEAEMRRLKLELKQTMEMYSTA 378

Query: 379 CKEALTAKQKAIELQRWKSEEERRIEEARLAEEAALEMVEQEKAKSRAAIEAAEAAKRIA 438
           CKEALTAKQKA ELQRWK EEER++EEAR AEEAAL + E+EKAKS+AA+EAAEAA+RIA
Sbjct: 379 CKEALTAKQKATELQRWKLEEERKLEEARNAEEAALAIAEKEKAKSKAAMEAAEAAQRIA 438

Query: 439 ELEAQKRINAEMKAFKEAEEKKKALDALAHNDVRYRKYSIEEIEAATEFFSESLKIGEGG 498
           ELEA+KR+NAEMKA KE+EEK KAL ALA++DVRYRKYSIE+IE ATEFF+E  KIGEGG
Sbjct: 439 ELEAKKRVNAEMKALKESEEKTKALTALANSDVRYRKYSIEDIELATEFFAEKYKIGEGG 498

Query: 499 YGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGCLVY 558
           YGPVYK YLDHTPVA+KVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPE GCLVY
Sbjct: 499 YGPVYKCYLDHTPVAVKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPECGCLVY 558

Query: 559 EFMANGSLDDCLFRRKENSKPLSWQLRFKIAAEIGTSLLFLHQAKPEPLVHRDLKPANIL 618
           EFMANGSL+D LF R  NS PLSWQ+RF+IAAEIGT LLFLHQAKPEPLVHRDLKP NIL
Sbjct: 559 EFMANGSLEDRLF-RLGNSPPLSWQMRFRIAAEIGTGLLFLHQAKPEPLVHRDLKPGNIL 618

Query: 619 LDRNYVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSL 678
           LDRN+VSKISDVGLARLVPP+VAD+VTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSL
Sbjct: 619 LDRNFVSKISDVGLARLVPPTVADTVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSL 678

Query: 679 GIMFLQLITAKPPMGLTHHVGRAIEKGTLLEFLDPSVTDWPAEESLCFAELALKCAELRR 738
           GIMFLQLITAKPPMGLTH+V RA+EKGTL++ LDP V+DWP E++  FA+LALKCAELRR
Sbjct: 679 GIMFLQLITAKPPMGLTHYVERALEKGTLVDLLDPVVSDWPMEDTEEFAKLALKCAELRR 738

Query: 739 KDRPDLRKVVLPELIRLRTIAEASMYSSVTDGVSSPEFSNSQIRLKTD-VSGESFS 773
           KDRPDL KV+LPEL RLRT+A+ S +S V      P  + SQ  LK + +SG S S
Sbjct: 739 KDRPDLAKVILPELNRLRTLADESSHSVVVGNSPLPSPTGSQSSLKLEHMSGASIS 785

BLAST of Lcy01g011020 vs. TAIR 10
Match: AT4G31230.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 893.6 bits (2308), Expect = 1.1e-259
Identity = 483/750 (64.40%), Postives = 592/750 (78.93%), Query Frame = 0

Query: 19  LVGIAIDKDKGSQNALKWATENLLIKGQTAILIHVKLRSSGSSLTSPKVMSATD-----N 78
           LV +AID+DK SQ ALKWA +NLL KGQT +L+HVK R+S  S T+P + S +      N
Sbjct: 17  LVAVAIDRDKNSQGALKWAVDNLLQKGQTVVLVHVKPRASSLS-TNPSINSNSSKTSQIN 76

Query: 79  GNDNVNVLVSKDIDPNTRDLFLPFRCFCTRKDIFCKDIVIEDVDVAKALIEYVSQAGIET 138
           G+ +   LV  + + + + LFLPFRC C+RKDI CKD+++E+ DVA+AL+EY +Q  IE 
Sbjct: 77  GDSS---LVCGEPEGSYKQLFLPFRCLCSRKDIQCKDVLLEESDVARALVEYANQVMIEV 136

Query: 139 LVVGTTTKGGFLRRFKTTDIPGSVIKGAPDFCNVYVISRGKIQTMRSASRPAPTSSPLRS 198
           LVVG+++KGGFLR  K  DIPG++ K APDFC VY I++GK+ T ++ASR AP+ SPLR 
Sbjct: 137 LVVGSSSKGGFLRFNKPIDIPGAITKTAPDFCTVYAITKGKLSTKKTASRAAPSVSPLR- 196

Query: 199 LLLSQSGSRSQLSESQISTIQRPEKPPIEPA-RRSQD--NIEFRSPFTRKGYNGKMPGEM 258
           + L Q+G +        +T  R E+   E   RRS D  +  FR P+ ++G  G+  GE+
Sbjct: 197 IQLQQNGLKPHPPLPSATTNTRAERQSFESQHRRSLDDQSDSFRPPYNKRGLTGRSYGEL 256

Query: 259 NLPDTDISFVSSGRPSVDKLFPTFFD-ADSGRIPPRLSNGTDGDIYNSFESQQFGRKSLD 318
           ++PD++ISF SSGRPS+++  P+ +D +D  R PPRLSN +D D Y SFES  FGR+S+D
Sbjct: 257 SIPDSEISFNSSGRPSIERNSPSLYDNSDPNRTPPRLSNFSDVD-YCSFESMTFGRRSMD 316

Query: 319 VNYPPEFSSVFSEGDRLSTSSQSMEEVEAEMRRLKLELKQTMEMYSTACKEALTAKQKAI 378
           ++ P  FS+   E +R S++SQ  ++VEAEMRRLKLELKQTMEMYSTACKEALTAK KA 
Sbjct: 317 LSSPTAFSTGSFENERFSSASQGGDDVEAEMRRLKLELKQTMEMYSTACKEALTAKHKAT 376

Query: 379 ELQRWKSEEERRIEEARLAEEAALEMVEQEKAKSRAAIEAAEAAKRIAELEAQKRINAEM 438
           ELQRWK  EER+ EEA+LAEEAAL + E+EKAKS+AA+EAAEAA+RIA++E++KR++AE 
Sbjct: 377 ELQRWKLAEERKFEEAKLAEEAALAIAEKEKAKSKAAMEAAEAAQRIADIESRKRVDAET 436

Query: 439 KAFKEAEEKKKALDALAHNDVRYRKYSIEEIEAATEFFSESLKIGEGGYGPVYKGYLDHT 498
           KA KE+E + KA++ALA  DVRYRKYSIEEIE ATEFF +  KIGEG YGPVYK YLDHT
Sbjct: 437 KALKESEARTKAVNALA-KDVRYRKYSIEEIEDATEFFDDKYKIGEGSYGPVYKCYLDHT 496

Query: 499 PVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGCLVYEFMANGSLDDCL 558
           PVA+K LRPDAAQGRSQFQ+EVEVL  IRHPNMVLLLGACPE GCLVYEFMANGSL+D L
Sbjct: 497 PVAVKALRPDAAQGRSQFQKEVEVLCSIRHPNMVLLLGACPECGCLVYEFMANGSLEDRL 556

Query: 559 FRRKENSKPLSWQLRFKIAAEIGTSLLFLHQAKPEPLVHRDLKPANILLDRNYVSKISDV 618
           FR+ + S  LSWQ RF+IAAEIGT LLFLHQ KPEPLVHRDLKPANILLDRN+VSK++DV
Sbjct: 557 FRQGD-SPALSWQTRFRIAAEIGTVLLFLHQTKPEPLVHRDLKPANILLDRNFVSKLADV 616

Query: 619 GLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITAKP 678
           GLARLVPPSVA++VTQY MTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLIT KP
Sbjct: 617 GLARLVPPSVANTVTQYHMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITGKP 676

Query: 679 PMGLTHHVGRAIEKGTLLEFLDPSVTDWPAEESLCFAELALKCAELRRKDRPDLRKVVLP 738
           PMGLTH+V RA+EKG L + LDP+V+DWP E++  FA+LALKCAE+RRKDRPDL KV+LP
Sbjct: 677 PMGLTHYVERALEKGNLKDLLDPAVSDWPVEDTTEFAKLALKCAEIRRKDRPDLSKVILP 736

Query: 739 ELIRLRTIAEASMYSSVTDGVSSPEFSNSQ 760
           EL RLR +AE S  ++V    S P  + SQ
Sbjct: 737 ELNRLRVLAEESTQAAVVINSSGPTPTESQ 758

BLAST of Lcy01g011020 vs. TAIR 10
Match: AT1G78940.2 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 755.0 bits (1948), Expect = 6.1e-218
Identity = 429/770 (55.71%), Postives = 550/770 (71.43%), Query Frame = 0

Query: 1   MWNQKPVKEKKEVVTGNELVGIAIDKDKGSQNALKWATENLLIKGQTAILIHVKLRSSGS 60
           MW  K    KKE  TG+  V +AIDKDKGSQ+ALKW  +NL  +GQT  LIHV  RS  S
Sbjct: 1   MWLPKANGAKKE--TGSGSVAVAIDKDKGSQHALKWTIDNLASRGQTISLIHVLCRSHSS 60

Query: 61  SLTSPKVMSATDNGNDNVNVLVSKDIDPNTRDLFLPFRCFCTRKDIFCKDIVIEDVDVAK 120
                   S  + G         + ++   +DLF+ F C+C+RK+I C+DI++ED D  +
Sbjct: 61  --------SDLEEGTPQ----QKQQMEKIAKDLFVSFHCYCSRKEINCRDILLEDADKVR 120

Query: 121 ALIEYVSQAGIETLVVGTTTKGGFLRRFKTTDIPGSVIKGAPDFCNVYVISRGKIQTMRS 180
           A+ EYVS + IE LVVG+ ++ GF+RRFK TD+P +V K APDFCNVYVIS+GKI ++R+
Sbjct: 121 AITEYVSSSAIENLVVGSASRNGFMRRFK-TDLPTTVSKSAPDFCNVYVISKGKIASVRN 180

Query: 181 ASRPAPTSSPLRSLLLSQSGSRSQLSESQISTIQRPEKPPIEPARRSQDNIEFRSPFT-R 240
           ASRPAP  + ++   +          +        P      P+R  +     RSP   R
Sbjct: 181 ASRPAPYQNSMQQCEI--DNHHPHTPDKAPKYHDHPNSAGSTPSRPRKSVEADRSPLVKR 240

Query: 241 KGYNGKMPGEMNLP--------DTDISFVSSGRPSVDKLFPTFFDADSGRIPPRLSNGTD 300
           K Y      + +L         D+DISF+SSGRPSV++   +    +S R   R+S  ++
Sbjct: 241 KPYGDFYDSDSDLSFISPSSHRDSDISFISSGRPSVERSSFSLDFPESAR-TSRMSTSSE 300

Query: 301 GDIYNSFESQQFGRKSLDVNYPPEFSSVFSE--GDRLSTSSQSMEEVEAEMRRLKLELKQ 360
                S  S + G K  D ++  E SS FSE  G   S SSQS+++VEAEM+RL+LELKQ
Sbjct: 301 ----QSIGSNRLGIKFSDPDFLNE-SSTFSEESGRTSSYSSQSLDDVEAEMKRLRLELKQ 360

Query: 361 TMEMYSTACKEALTAKQKAIELQRWKSEEERRIEEARLAEEAALEMVEQEKAKSRAAIEA 420
           TM+MYSTACKEAL+A+Q+A ELQ+ ++EEERR+EEA+ +EEAA+ +VE+E+AK++AA+EA
Sbjct: 361 TMDMYSTACKEALSARQQATELQKLRTEEERRLEEAKSSEEAAMSIVEKERAKAKAALEA 420

Query: 421 AEAAKRIAELEAQKRINAEMKAFKEAEEKKKALDALAHNDVRYRKYSIEEIEAATEFFSE 480
           AEAAKR+AE+E+++R+ AEMK  KE+       D+ +   VRYRKY+++EIE AT  F+E
Sbjct: 421 AEAAKRLAEVESKRRLTAEMKTMKES-------DSFSRGFVRYRKYTVDEIEEATSNFAE 480

Query: 481 SLKIGEGGYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGAC 540
           S K+GEGGYGPV++G+LDHT VA+KVLRPDAAQGRSQFQ+EVEVLSCIRHPNMVLLLGAC
Sbjct: 481 SQKVGEGGYGPVFRGFLDHTSVAVKVLRPDAAQGRSQFQKEVEVLSCIRHPNMVLLLGAC 540

Query: 541 PEYGCLVYEFMANGSLDDCLFRRKENSKPLSWQLRFKIAAEIGTSLLFLHQAKPEPLVHR 600
           PE+G LVYE+MA GSL+D LF R  N+ P++WQLRF+IAAEI T LLFLHQ KPEP+VHR
Sbjct: 541 PEFGILVYEYMAKGSLEDRLFMR-GNTPPITWQLRFRIAAEIATGLLFLHQTKPEPIVHR 600

Query: 601 DLKPANILLDRNYVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLG 660
           DLKP N+LLD NYVSKISDVGLARLV P+VA++VTQYR+TS AGTFCYIDPEYQQTGMLG
Sbjct: 601 DLKPGNVLLDYNYVSKISDVGLARLV-PAVAENVTQYRVTSAAGTFCYIDPEYQQTGMLG 660

Query: 661 VKSDIYSLGIMFLQLITAKPPMGLTHHVGRAIEKGTLLEFLDPSVTDWPAEESLCFAELA 720
           VKSD+YSLGIM LQ++TAK PMGL ++V +AIE+GTL + LDP+V DWP EE+L  A+L+
Sbjct: 661 VKSDVYSLGIMLLQILTAKQPMGLAYYVEQAIEEGTLKDMLDPAVPDWPIEEALSLAKLS 720

Query: 721 LKCAELRRKDRPDLRKVVLPELIRLRTIAEASMYSSVTDGVSSPEFSNSQ 760
           L+CAELRRKDRPDL K +LPEL RLR I E S+ S    G      + SQ
Sbjct: 721 LQCAELRRKDRPDLGKEILPELNRLREIGEESLESVFYAGNQGKSPNTSQ 738

BLAST of Lcy01g011020 vs. TAIR 10
Match: AT5G35380.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 753.1 bits (1943), Expect = 2.3e-217
Identity = 431/744 (57.93%), Postives = 540/744 (72.58%), Query Frame = 0

Query: 9   EKKEVVTGNELVGIAIDKDKGSQNALKWATENLLIKGQTAILIHVKLRS------SGSSL 68
           EK+E     E + +AID+DK SQNALKWA  NLL +GQT  L+HVKL+       SGS+ 
Sbjct: 6   EKRE-----ENITLAIDRDKESQNALKWAVSNLLSRGQTLTLLHVKLKQPSSLPYSGSNF 65

Query: 69  TSPKVMSATDNGNDNVNVLVSKDIDPNTRDLFLPFRCFCTRKDIFCKDIVIEDVDVAKAL 128
           + P        G+D               +LFLPFRC+C RKDI C+D+V+EDV  AK +
Sbjct: 66  SKP--------GDD-------------PSELFLPFRCYCARKDINCQDVVVEDVSAAKGI 125

Query: 129 IEYVSQAGIETLVVGTTTKGGFLRRFKTTDIPGSVIKGAPDFCNVYVISRGKIQTMRSAS 188
           ++YV Q  IETL++G++     L RFK  D+  +V+K AP FC VYVIS+GKI  +RSA 
Sbjct: 126 VDYVQQNAIETLILGSSKM--TLLRFKAADVSSTVMKKAPSFCTVYVISKGKISFLRSA- 185

Query: 189 RPAPTSSPLRSLLLS-QSGSRSQLSESQISTIQRPEKPPIEPARRSQDNIEFRSPFTRKG 248
               TSSP  S + S +  S +Q S   +   Q       +  +R+ D I+      ++G
Sbjct: 186 ----TSSPPHSNMPSMRHHSHAQTSNMNVERRQ-------QTMQRTHDEIKIE---IKRG 245

Query: 249 YNGKMPGEMNLPDTDISFVSSGRPSVDKLFPTFFDADSGRIPPRLSNGTD-GDIYNSFES 308
           Y G    + ++ D+DISFVSSGRPSVD++FP+ +D       PRLS  ++ G+   SF +
Sbjct: 246 YEGVY--QPSITDSDISFVSSGRPSVDQMFPSLYDDVD---VPRLSVTSEYGENRLSF-A 305

Query: 309 QQFGRKSLDVN--YPPEFSSVFSEGDRLSTSSQSMEEVEAEMRRLKLELKQTMEMYSTAC 368
             + ++S+D+   Y P  S+ F  G R S S Q  +E+E EMRRLK+ELK TMEMY++AC
Sbjct: 306 TTYSKQSIDLGSPYAPNSSTSFESG-RQSFSLQGQDELETEMRRLKMELKHTMEMYNSAC 365

Query: 369 KEALTAKQKAIELQRWKSEEERRIEEARLAEEAALEMVEQEKAKSRAAIEAAEAAKRIAE 428
           KEA++AK+ A EL +WK+E+E ++EE RL++EAA+ M E+EK KSRAA+EAA AA+++++
Sbjct: 366 KEAISAKKAANELLKWKAEKEHKLEEVRLSKEAAMAMAEREKEKSRAAMEAAVAAQKLSD 425

Query: 429 LEAQKRINAEMKAFKEAEEKKKALDALAHNDVRYRKYSIEEIEAATEFFSESLKIGEGGY 488
           LEA+KR     K  +  +EKK+A+ +L     RYRKY+IEEIE ATE FS S K+GEGGY
Sbjct: 426 LEAEKR-----KHIETVDEKKRAVSSL-----RYRKYTIEEIEEATEDFSPSRKVGEGGY 485

Query: 489 GPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLSCIRHPNMVLLLGACPEYGCLVYE 548
           GPVYKG LD+T VAIKVLRPDAAQGRSQFQ+EVEVL+C+RHPNMVLLLGACPEYGCLVYE
Sbjct: 486 GPVYKGTLDYTKVAIKVLRPDAAQGRSQFQREVEVLTCMRHPNMVLLLGACPEYGCLVYE 545

Query: 549 FMANGSLDDCLFRRKENSKPLSWQLRFKIAAEIGTSLLFLHQAKPEPLVHRDLKPANILL 608
           +MANGSLDDCLFRR  NS  LSWQLRF+IA+EI T L FLHQ KPEPLVHRDLKP NILL
Sbjct: 546 YMANGSLDDCLFRR-GNSPILSWQLRFRIASEIATGLHFLHQMKPEPLVHRDLKPGNILL 605

Query: 609 DRNYVSKISDVGLARLVPPSVADSVTQYRMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLG 668
           D+++VSKISDVGLARLVPPSVAD+ TQYRMTSTAGTF YIDPEYQQTGMLG KSDIYS G
Sbjct: 606 DQHFVSKISDVGLARLVPPSVADTATQYRMTSTAGTFFYIDPEYQQTGMLGTKSDIYSFG 665

Query: 669 IMFLQLITAKPPMGLTHHVGRAIEKGTLLEFLDPSVTDWPAEESLCFAELALKCAELRRK 728
           IM LQ++TAKPPMGLTHHV +AIEKGT  E LDP+V DWP EE+L  A+LAL+CA+LRRK
Sbjct: 666 IMLLQILTAKPPMGLTHHVEKAIEKGTFAEMLDPAVPDWPFEEALAAAKLALQCAKLRRK 688

Query: 729 DRPDLRKVVLPELIRLRTIAEASM 743
           DRPDL  +VLPEL +LR +AE S+
Sbjct: 726 DRPDLGNIVLPELKKLRDLAEESI 688

BLAST of Lcy01g011020 vs. TAIR 10
Match: AT1G16760.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 751.5 bits (1939), Expect = 6.8e-217
Identity = 428/769 (55.66%), Postives = 539/769 (70.09%), Query Frame = 0

Query: 1   MWNQKPVKEKKEVVTGNELVGIAIDKDKGSQNALKWATENLLIKGQTAILIHVKLRSSGS 60
           MW  K    KK   T N  V IAIDKDK SQNA+KW  ENL  +GQT  LIHV       
Sbjct: 1   MWLPKADATKKG--TRNGSVAIAIDKDKSSQNAIKWTLENLATRGQTLALIHV------- 60

Query: 61  SLTSPKVMSATDNGNDNVNVLVSKDIDPNTRDLFLPFRCFCTRKDIFCKDIVIEDVDVAK 120
               PK  S+ D       +   + ++  T+DLF+ F C+C+RK+I C D+V+EDVD  K
Sbjct: 61  ---LPKSQSSLD---IEEGITHKQQMEKQTKDLFVSFHCYCSRKEIHCLDVVLEDVDKVK 120

Query: 121 ALIEYVSQAGIETLVVGTTTKGGFLRRFKTTDIPGSVIKGAPDFCNVYVISRGKIQTMRS 180
           A++EYV+ + IE LV+G  ++  F+RRFK TD+P SV K APDFCNVYVIS+GKI ++R+
Sbjct: 121 AIVEYVTVSAIENLVLGAPSRNSFMRRFK-TDLPTSVSKAAPDFCNVYVISKGKISSLRN 180

Query: 181 ASRPAPTSSPLRSLLLSQSGSRSQLSESQISTIQRPEK----PPIEPARRSQDNIEFRSP 240
           ASR AP    +           S++ + +   I+R  K    P +   RRS D     S 
Sbjct: 181 ASRLAPYHPSV----------LSEVDDHETIAIERKHKTANTPALPKGRRSID-----SD 240

Query: 241 FTRKGYNGKMPGEMNL-------------------PDTDISFVSSGRPSVDKLFPTFFDA 300
            TR G      G M L                     +D+SF+SSGRPSVD+   T+   
Sbjct: 241 VTRLGLPKPPHGHMKLMGDFSDSESEFSFISASQQESSDLSFISSGRPSVDRSSFTYDLP 300

Query: 301 DSGRIPPRLSNGTDGDIYNSFESQQFGRKSLDVNYPPEFSSVFSEGDRLST--SSQSMEE 360
           +S R   R+S  ++     S  S + G K  D++Y    SSV  E  R S   SSQS+ +
Sbjct: 301 ESAR-TSRMSTSSE----QSIGSHRLGIKFTDLSYLNGSSSVSDESGRTSCSFSSQSLND 360

Query: 361 VEAEMRRLKLELKQTMEMYSTACKEALTAKQKAIELQRWKSEEERRIEEARLAEEAALEM 420
           VEA+M+RL+LELKQTM+MYS+AC+EALTA+ +A ELQ+ ++EEERR+EE ++ EE A+ +
Sbjct: 361 VEAQMKRLRLELKQTMDMYSSACREALTARNEATELQKLRTEEERRLEELKMTEETAMSI 420

Query: 421 VEQEKAKSRAAIEAAEAAKRIAELEAQKRINAEMKAFKEAEEKKKALDALAHNDVRYRKY 480
           VE E+AK++ A+EAAEAA R+AE+EA++R++AEMK  KE++   +      H+ VRYRKY
Sbjct: 421 VENERAKAKTALEAAEAANRLAEVEAKRRVHAEMKVLKESDSFSR------HSIVRYRKY 480

Query: 481 SIEEIEAATEFFSESLKIGEGGYGPVYKGYLDHTPVAIKVLRPDAAQGRSQFQQEVEVLS 540
           S++EIE  T  F+ES K+GEGGYGPV++G+LDHT VA+KVLRPDAAQGRSQF +EVEVLS
Sbjct: 481 SVQEIEEGTANFAESRKVGEGGYGPVFRGHLDHTSVAVKVLRPDAAQGRSQFHKEVEVLS 540

Query: 541 CIRHPNMVLLLGACPEYGCLVYEFMANGSLDDCLFRRKENSKPLSWQLRFKIAAEIGTSL 600
           CIRHPNMVLLLGACPEYG LVYE+MA GSLDD LFRR  N+ P+SWQLRF+IAAEI T L
Sbjct: 541 CIRHPNMVLLLGACPEYGILVYEYMARGSLDDRLFRR-GNTPPISWQLRFRIAAEIATGL 600

Query: 601 LFLHQAKPEPLVHRDLKPANILLDRNYVSKISDVGLARLVPPSVADSVTQYRMTSTAGTF 660
           LFLHQ KPEP+VHRDLKP N+LLD NYVSKISDVGLARLV P+VA++VTQYR+TS AGTF
Sbjct: 601 LFLHQTKPEPIVHRDLKPGNVLLDHNYVSKISDVGLARLV-PAVAENVTQYRVTSAAGTF 660

Query: 661 CYIDPEYQQTGMLGVKSDIYSLGIMFLQLITAKPPMGLTHHVGRAIEKGTLLEFLDPSVT 720
           CYIDPEYQQTGMLGVKSD+YSLGIM LQL+TAK PMGL ++V +AIE+GTL + LDP+V 
Sbjct: 661 CYIDPEYQQTGMLGVKSDVYSLGIMLLQLLTAKQPMGLAYYVEQAIEEGTLKDMLDPAVP 720

Query: 721 DWPAEESLCFAELALKCAELRRKDRPDLRKVVLPELIRLRTIAEASMYS 745
           DWP EE+L  A+L+L+CAELRRKDRPDL K V+PEL RLR I E S+ S
Sbjct: 721 DWPLEEALSLAKLSLQCAELRRKDRPDLGKEVMPELSRLREIGEESLDS 725