Lcy01g010470 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy01g010470
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	RNase H domain-containing protein
Location	Chr01: 30079359 .. 30079943 (+)
RNA-Seq Expression	Lcy01g010470
Synteny	Lcy01g010470

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTCCATAATAGGCCAATTCCATCCATCGATGTTCGGTGGTGTACCTGGATTTGTGATTACTTGAATGAGTTTACTTTAGTAAACAAGTGTCCTTCTAAGCGTGTTCAACAAGTCAATGAAGTTAGATCTCTTCTTCAAGGGAGGGATGCAATGATTATTCATGTGGATGCGGCTTGTGATACGAGTGGGCGACGCGCTAGTGTTGGTGTGGGAATTCGAAATTCTCGAGGTCGATTGATAGCGACACTGAATAGACTTTTAGAGACGTTTGTCAACCCATTATGTGCAGAGGAATACGCAATGTTAGAGGGACTTCATTTGGCCAATCGAATGAACCTTAGGAGGATTACAATTTTCTCCGATTCGCTCTCTTTGGTGATGATGTTGAGAAAGGAGACGACAGTTTGTGTTGATGTGGCTTCTATCATTTGGGATGTTGACATGATAAAAAGATGTTTTGAGTTTGTAGAGATCGGCTATGTGAGTCGAAATTCTAATGGTATGGCTCATACATTGGCTCGTCAAAGTCTTTATGATGGATCGATGTTGTGGTTATCAAATTTTCCTTCTTGGCTATCCTAA

mRNA sequence

ATGGTCCATAATAGGCCAATTCCATCCATCGATGTTCGGTGGTGTACCTGGATTTGTGATTACTTGAATGAGTTTACTTTAGTAAACAAGTGTCCTTCTAAGCGTGTTCAACAAGTCAATGAAGTTAGATCTCTTCTTCAAGGGAGGGATGCAATGATTATTCATGTGGATGCGGCTTGTGATACGAGTGGGCGACGCGCTAGTGTTGGTGTGGGAATTCGAAATTCTCGAGGTCGATTGATAGCGACACTGAATAGACTTTTAGAGACGTTTGTCAACCCATTATGTGCAGAGGAATACGCAATGTTAGAGGGACTTCATTTGGCCAATCGAATGAACCTTAGGAGGATTACAATTTTCTCCGATTCGCTCTCTTTGGTGATGATGTTGAGAAAGGAGACGACAGTTTGTGTTGATGTGGCTTCTATCATTTGGGATGTTGACATGATAAAAAGATGTTTTGAGTTTGTAGAGATCGGCTATGTGAGTCGAAATTCTAATGGTATGGCTCATACATTGGCTCGTCAAAGTCTTTATGATGGATCGATGTTGTGGTTATCAAATTTTCCTTCTTGGCTATCCTAA

Coding sequence (CDS)

ATGGTCCATAATAGGCCAATTCCATCCATCGATGTTCGGTGGTGTACCTGGATTTGTGATTACTTGAATGAGTTTACTTTAGTAAACAAGTGTCCTTCTAAGCGTGTTCAACAAGTCAATGAAGTTAGATCTCTTCTTCAAGGGAGGGATGCAATGATTATTCATGTGGATGCGGCTTGTGATACGAGTGGGCGACGCGCTAGTGTTGGTGTGGGAATTCGAAATTCTCGAGGTCGATTGATAGCGACACTGAATAGACTTTTAGAGACGTTTGTCAACCCATTATGTGCAGAGGAATACGCAATGTTAGAGGGACTTCATTTGGCCAATCGAATGAACCTTAGGAGGATTACAATTTTCTCCGATTCGCTCTCTTTGGTGATGATGTTGAGAAAGGAGACGACAGTTTGTGTTGATGTGGCTTCTATCATTTGGGATGTTGACATGATAAAAAGATGTTTTGAGTTTGTAGAGATCGGCTATGTGAGTCGAAATTCTAATGGTATGGCTCATACATTGGCTCGTCAAAGTCTTTATGATGGATCGATGTTGTGGTTATCAAATTTTCCTTCTTGGCTATCCTAA

Protein sequence

MVHNRPIPSIDVRWCTWICDYLNEFTLVNKCPSKRVQQVNEVRSLLQGRDAMIIHVDAACDTSGRRASVGVGIRNSRGRLIATLNRLLETFVNPLCAEEYAMLEGLHLANRMNLRRITIFSDSLSLVMMLRKETTVCVDVASIIWDVDMIKRCFEFVEIGYVSRNSNGMAHTLARQSLYDGSMLWLSNFPSWLS

Homology

[Close]

BLAST of Lcy01g010470 vs. ExPASy TrEMBL
Match: A0A5C7GQX3 (RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_026488 PE=4 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 3.3e-11
Identity = 55/176 (31.25%), Postives = 87/176 (49.43%), Query Frame = 0


Query: 17  WICDYLNEFTLVNKCPSKRVQQVNEVRSLLQGRDAMI-IHVDAACDTSGRRASVGVGIRN 76
           W  D+L EF   +     R+     V   L     M  I+ DAA +  G  A +GV IRN
Sbjct: 53  WAGDFLQEFRKSSAPVQPRLVSDRPVPKWLAPPQGMFKINTDAALNVQGNSAGIGVVIRN 112

Query: 77  SRGRLIATLNRLLETFVNPLCAEEYAMLEGLHLANRMNLRRITIFSDSLSLVMMLRKETT 136
            RG+++ +  +   T  +P+ AE  A+LEGL LA         + SD+L++V  + +  +
Sbjct: 113 FRGQVMLSSCKNFRTAFSPVIAEALAVLEGLRLAKLGGFFPTVLESDALTVVQDICRNES 172

Query: 137 VCVDVASIIWDVDMIKRCFEFVEIGYVSRNSNGMAHTLARQSLYD-GSMLWLSNFP 191
           +  DV  +I D+    R F F    +V R +N +AH LA+ +L + G  +W  + P
Sbjct: 173 LSSDVGLVIDDILFFCRDFNFFSFRHVPRLANKVAHGLAKLALSNLGEFVWSGDCP 228

[Close]

BLAST of Lcy01g010470 vs. ExPASy TrEMBL
Match: A0A1E5ULW7 (RNase H domain-containing protein OS=Dichanthelium oligosanthes OX=888268 GN=BAE44_0025101 PE=4 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 1.2e-10
Identity = 50/126 (39.68%), Postives = 67/126 (53.17%), Query Frame = 0


Query: 65  RRASVGVGIRNSRGRLIATLNRLLETFVNPLCAEEYAMLEGLHLANRMNLRRITIFSDSL 124
           RRAS+G  IR+S GRL+       E     +  E  AML GL LA ++ L R+    D L
Sbjct: 60  RRASIGGAIRDSAGRLVLAFAEKTEHSTVGI-VEARAMLRGLQLAGQLGLERVVAEGDDL 119

Query: 125 SLVMMLRKETTVCVDVASIIWDVDMIKRCFEFVEIGYVSRNSNGMAHTLARQSLYDGSML 184
            LV +LR E T      +I  ++  + RCF   +I +V R  N +AHTL RQ+ Y GS +
Sbjct: 120 VLVQLLRGEETQTRIPVAIQEEILGVLRCFSSCDIQHVFREGNQVAHTLCRQA-YHGSGM 179

Query: 185 WLSNFP 191
           WL   P
Sbjct: 180 WLGGVP 183

[Close]

BLAST of Lcy01g010470 vs. ExPASy TrEMBL
Match: A0A7J8W9I3 (RNase H domain-containing protein OS=Gossypium klotzschianum OX=34286 GN=Goklo_024863 PE=4 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 2.8e-10
Identity = 41/133 (30.83%), Postives = 72/133 (54.14%), Query Frame = 0


Query: 54  IHVDAACDTSGRRASVGVGIRNSRGRLIATLNRLLETFVNPLCAEEYAMLEGLHLANRMN 113
           IH DAA D  G R++ G+  RN    ++A+         +P  AE YA L+ + L  R+ 
Sbjct: 262 IHFDAAYDRLGFRSASGIIARNENREILASQAVTHSNIADPFTAEAYAGLQAIKLGIRLG 321

Query: 114 LRRITIFSDSLSLVMMLRKETTVCVDVASIIWDVDMIKRCFEFVEIGYVSRNSNGMAHTL 173
           + ++T+  DS +++   +   TV   + +II D+  +K+ F+ +E  +V +  N  AHT+
Sbjct: 322 VNKLTVLGDSKTVIKKCQSLFTVKSVIGAIITDIQSLKKGFQEIEFIFVPKEHNFYAHTI 381

Query: 174 ARQSLYDGSMLWL 187
           A++SL  G   +L
Sbjct: 382 AKESLRSGESFYL 394

[Close]

BLAST of Lcy01g010470 vs. ExPASy TrEMBL
Match: A0A6J1DJL6 (uncharacterized protein LOC111021711 OS=Momordica charantia OX=3673 GN=LOC111021711 PE=4 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 4.7e-10
Identity = 44/146 (30.14%), Postives = 77/146 (52.74%), Query Frame = 0


Query: 50  DAMIIHVDAACDTSGRRASVGVGIRNSRGRLIATLNRLLETFVNPLCAEEYAMLEGLHLA 109
           D + I+VD AC  S  R  +GV  +N +G +I  + R L+ F  PL  E  A+LEGL L 
Sbjct: 134 DWVKINVDTACKLSNSRTGIGVVCQNDKGEIIVAMIRKLDVFYEPLQVETLAILEGLWLT 193

Query: 110 NRMNLRRITIFSDSLSLVMMLRKETTVCVDVASIIWDVDMIKRCFEFVEIGYVSRNSNGM 169
             ++L  + + SDS + + ++ +E          + D+ M    F  +   ++ R+S+ +
Sbjct: 194 QCLDLIFVQVDSDSQNSINLISQEYDPSHQPNVWVDDIHMCAINFIDILFMHIQRSSSNV 253

Query: 170 AHTLARQSLYDG-SMLWLSNFPSWLS 195
            H LA  ++    + +W+S FP+WL+
Sbjct: 254 THRLASTAISSNPNKIWISRFPNWLT 279

[Close]

BLAST of Lcy01g010470 vs. ExPASy TrEMBL
Match: A0A5C7IFK3 (RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_009054 PE=4 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 8.0e-10
Identity = 45/141 (31.91%), Postives = 78/141 (55.32%), Query Frame = 0


Query: 54  IHVDAACDTSGRRASVGVGIRNSRGRLIATLNRLLETFVNPLCAEEYAMLEGLHLANRMN 113
           I+VDAA D    R S G+ IR+S GR++     + +   +   AE  A+ EG+ LA    
Sbjct: 144 INVDAALDVGRNRFSAGIVIRDSSGRILKAAALVFKGRTSVDIAEAKAIYEGILLATTSG 203

Query: 114 LRRITIFSDSLSLVMMLRKETTVCVDVASIIWDVDMIKRCFEFVEIGYVSRNSNGMAHTL 173
           L+ ++I SDSL++V +   E +   DV ++I D+  +      + I Y+ R+ N +AH +
Sbjct: 204 LQSLSIESDSLNVVRLCNGEISTRNDVFNVICDIQFLLSRERKISISYIPRSCNMVAHEV 263

Query: 174 ARQSL-YDGSMLWLSNFPSWL 194
           AR+++  + S++    +P WL
Sbjct: 264 ARRAIGLENSVIMYDPYPIWL 284

[Close]

BLAST of Lcy01g010470 vs. NCBI nr
Match: TXG47194.1 (hypothetical protein EZV62_026488 [Acer yangbiense])

HSP 1 Score: 78.6 bits (192), Expect = 6.7e-11
Identity = 55/176 (31.25%), Postives = 87/176 (49.43%), Query Frame = 0


Query: 17  WICDYLNEFTLVNKCPSKRVQQVNEVRSLLQGRDAMI-IHVDAACDTSGRRASVGVGIRN 76
           W  D+L EF   +     R+     V   L     M  I+ DAA +  G  A +GV IRN
Sbjct: 53  WAGDFLQEFRKSSAPVQPRLVSDRPVPKWLAPPQGMFKINTDAALNVQGNSAGIGVVIRN 112

Query: 77  SRGRLIATLNRLLETFVNPLCAEEYAMLEGLHLANRMNLRRITIFSDSLSLVMMLRKETT 136
            RG+++ +  +   T  +P+ AE  A+LEGL LA         + SD+L++V  + +  +
Sbjct: 113 FRGQVMLSSCKNFRTAFSPVIAEALAVLEGLRLAKLGGFFPTVLESDALTVVQDICRNES 172

Query: 137 VCVDVASIIWDVDMIKRCFEFVEIGYVSRNSNGMAHTLARQSLYD-GSMLWLSNFP 191
           +  DV  +I D+    R F F    +V R +N +AH LA+ +L + G  +W  + P
Sbjct: 173 LSSDVGLVIDDILFFCRDFNFFSFRHVPRLANKVAHGLAKLALSNLGEFVWSGDCP 228

[Close]

BLAST of Lcy01g010470 vs. NCBI nr
Match: OEL13880.1 (hypothetical protein BAE44_0025101 [Dichanthelium oligosanthes])

HSP 1 Score: 76.6 bits (187), Expect = 2.6e-10
Identity = 50/126 (39.68%), Postives = 67/126 (53.17%), Query Frame = 0


Query: 65  RRASVGVGIRNSRGRLIATLNRLLETFVNPLCAEEYAMLEGLHLANRMNLRRITIFSDSL 124
           RRAS+G  IR+S GRL+       E     +  E  AML GL LA ++ L R+    D L
Sbjct: 60  RRASIGGAIRDSAGRLVLAFAEKTEHSTVGI-VEARAMLRGLQLAGQLGLERVVAEGDDL 119

Query: 125 SLVMMLRKETTVCVDVASIIWDVDMIKRCFEFVEIGYVSRNSNGMAHTLARQSLYDGSML 184
            LV +LR E T      +I  ++  + RCF   +I +V R  N +AHTL RQ+ Y GS +
Sbjct: 120 VLVQLLRGEETQTRIPVAIQEEILGVLRCFSSCDIQHVFREGNQVAHTLCRQA-YHGSGM 179

Query: 185 WLSNFP 191
           WL   P
Sbjct: 180 WLGGVP 183

[Close]

BLAST of Lcy01g010470 vs. NCBI nr
Match: MBA0671686.1 (hypothetical protein [Gossypium klotzschianum])

HSP 1 Score: 75.5 bits (184), Expect = 5.7e-10
Identity = 41/133 (30.83%), Postives = 72/133 (54.14%), Query Frame = 0


Query: 54  IHVDAACDTSGRRASVGVGIRNSRGRLIATLNRLLETFVNPLCAEEYAMLEGLHLANRMN 113
           IH DAA D  G R++ G+  RN    ++A+         +P  AE YA L+ + L  R+ 
Sbjct: 262 IHFDAAYDRLGFRSASGIIARNENREILASQAVTHSNIADPFTAEAYAGLQAIKLGIRLG 321

Query: 114 LRRITIFSDSLSLVMMLRKETTVCVDVASIIWDVDMIKRCFEFVEIGYVSRNSNGMAHTL 173
           + ++T+  DS +++   +   TV   + +II D+  +K+ F+ +E  +V +  N  AHT+
Sbjct: 322 VNKLTVLGDSKTVIKKCQSLFTVKSVIGAIITDIQSLKKGFQEIEFIFVPKEHNFYAHTI 381

Query: 174 ARQSLYDGSMLWL 187
           A++SL  G   +L
Sbjct: 382 AKESLRSGESFYL 394

[Close]

BLAST of Lcy01g010470 vs. NCBI nr
Match: XP_022154440.1 (uncharacterized protein LOC111021711 [Momordica charantia])

HSP 1 Score: 74.7 bits (182), Expect = 9.7e-10
Identity = 44/146 (30.14%), Postives = 77/146 (52.74%), Query Frame = 0


Query: 50  DAMIIHVDAACDTSGRRASVGVGIRNSRGRLIATLNRLLETFVNPLCAEEYAMLEGLHLA 109
           D + I+VD AC  S  R  +GV  +N +G +I  + R L+ F  PL  E  A+LEGL L 
Sbjct: 134 DWVKINVDTACKLSNSRTGIGVVCQNDKGEIIVAMIRKLDVFYEPLQVETLAILEGLWLT 193

Query: 110 NRMNLRRITIFSDSLSLVMMLRKETTVCVDVASIIWDVDMIKRCFEFVEIGYVSRNSNGM 169
             ++L  + + SDS + + ++ +E          + D+ M    F  +   ++ R+S+ +
Sbjct: 194 QCLDLIFVQVDSDSQNSINLISQEYDPSHQPNVWVDDIHMCAINFIDILFMHIQRSSSNV 253

Query: 170 AHTLARQSLYDG-SMLWLSNFPSWLS 195
            H LA  ++    + +W+S FP+WL+
Sbjct: 254 THRLASTAISSNPNKIWISRFPNWLT 279

[Close]

BLAST of Lcy01g010470 vs. NCBI nr
Match: TXG67779.1 (hypothetical protein EZV62_009054 [Acer yangbiense])

HSP 1 Score: 73.9 bits (180), Expect = 1.7e-09
Identity = 45/141 (31.91%), Postives = 78/141 (55.32%), Query Frame = 0


Query: 54  IHVDAACDTSGRRASVGVGIRNSRGRLIATLNRLLETFVNPLCAEEYAMLEGLHLANRMN 113
           I+VDAA D    R S G+ IR+S GR++     + +   +   AE  A+ EG+ LA    
Sbjct: 144 INVDAALDVGRNRFSAGIVIRDSSGRILKAAALVFKGRTSVDIAEAKAIYEGILLATTSG 203

Query: 114 LRRITIFSDSLSLVMMLRKETTVCVDVASIIWDVDMIKRCFEFVEIGYVSRNSNGMAHTL 173
           L+ ++I SDSL++V +   E +   DV ++I D+  +      + I Y+ R+ N +AH +
Sbjct: 204 LQSLSIESDSLNVVRLCNGEISTRNDVFNVICDIQFLLSRERKISISYIPRSCNMVAHEV 263

Query: 174 ARQSL-YDGSMLWLSNFPSWL 194
           AR+++  + S++    +P WL
Sbjct: 264 ARRAIGLENSVIMYDPYPIWL 284

The following BLAST results are available for this feature:

BLAST of Lcy01g010470 vs.


Match Name	E-value	Identity	Description

BLAST of Lcy01g010470 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5C7GQX3	3.3e-11	31.25	RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_026488 ...	[more]
A0A1E5ULW7	1.2e-10	39.68	RNase H domain-containing protein OS=Dichanthelium oligosanthes OX=888268 GN=BAE...	[more]
A0A7J8W9I3	2.8e-10	30.83	RNase H domain-containing protein OS=Gossypium klotzschianum OX=34286 GN=Goklo_0...	[more]
A0A6J1DJL6	4.7e-10	30.14	uncharacterized protein LOC111021711 OS=Momordica charantia OX=3673 GN=LOC111021...	[more]
A0A5C7IFK3	8.0e-10	31.91	RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_009054 ...	[more]

BLAST of Lcy01g010470 vs. NCBI nr


Match Name	E-value	Identity	Description
TXG47194.1	6.7e-11	31.25	hypothetical protein EZV62_026488 [Acer yangbiense]	[more]
OEL13880.1	2.6e-10	39.68	hypothetical protein BAE44_0025101 [Dichanthelium oligosanthes]	[more]
MBA0671686.1	5.7e-10	30.83	hypothetical protein [Gossypium klotzschianum]	[more]
XP_022154440.1	9.7e-10	30.14	uncharacterized protein LOC111021711 [Momordica charantia]	[more]
TXG67779.1	1.7e-09	31.91	hypothetical protein EZV62_009054 [Acer yangbiense]	[more]

BLAST of Lcy01g010470 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 51..181 e-value: 4.2E-16 score: 61.1
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 56..176 e-value: 1.3E-19 score: 70.2
None	No IPR available	PANTHER	PTHR33033	POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATED	coord: 54..192
None	No IPR available	PANTHER	PTHR33033:SF67	SUBFAMILY NOT NAMED	coord: 54..192
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 54..175 e-value: 3.67571E-16 score: 69.2652
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 51..178

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy01g010470.1	Lcy01g010470.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity