Lcy01g010060 (gene) Sponge gourd (P93075) v1

Overview
NameLcy01g010060
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionBOI-related E3 ubiquitin-protein ligase 1
LocationChr01: 28974018 .. 28977022 (-)
RNA-Seq ExpressionLcy01g010060
SyntenyLcy01g010060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCTCTCTCTCTCTCCTATAATTCTTCATCTTCATCTTCTTCTTCTTCTTCTTCTTCGTCGGTTACCACTCCAAAAGCCTTCATCCGTCTCTAGATCCTATTCTTCCTCCTTCATCATTCTCCTGCCCTTTTCATTCTACGTATTCCTATGCATATCTTCAAGGTATTCTCTCTTTGATTTCTGTTTCTGTCTCTGTGTTTATATCTGTGTTTCTGCCAATTATGTCGTTCAATCTCTGTGGATTGTTGTGTGGCAGGATCTGGAGATGATATCTGTTCTAATCTGAAACTGCCTGAATGACTTCTTCTTGAGGTAATCTCTATGCCTTTTACTTTCTTGCCTTCCGTGTTTCTCACCGTAGCTTGTGTTTGCGTCGTTGGTAAAGTCGTGCCTCATAAGGAAAACTTCGTATTCGTAATGAATCCGTGGATGATGATCTGAAGCAAACGTTTTTTCTTCTCAAATTTGTTAGATTATCTGTTACTTTCTTAGTTAAATCTTTTTTGTTTTTCATCTTCATTATGCGATTCCGGTCAAACTTGCTCATCAATCTCCGTAACTTTCCTTATTTAGAGATCCGAGCACTTCTGATGCACTCAACTTTGTATGTTTTCCCATTCCATATCCGTATGCCTTTCCCTGTTGATGTTTTTCCTGACTATCTGGATATGGTTTTGATATAGAACTCCTGAGATTATCCTCTGATCTGATGTATTTCTAGTTTTATTTCTGAAATTCGTGCTTTCCTTGAATGTGATGTTTATGTTTGACTTTCTGTTATTGAGGTTTGTAAGGAAACCATGATGATGGGTGGCTTACCGGATTGTATTTTCTCCAAATGGACAGATTGTAGTTCTAAGTAAATGTTATGAATTGCTGGATTTTAGAAGATCACTGATTTCCCATTATTGGAGATTCAAATGCTTAATTAAGTTCAGTCATGGATTATTTTACCCATGTATTGTAATAGGATATGTTGAAGATGTAAGGCCACTATTGTTATGTTATATTTGGGAAAAATTTAATGGAACAGCTGTTGTGACAATGGCGAGTTTATTTTAGAACTTGTTTCATCCAAGATTTTTGGCATTTTTGCAAATTGATTGATTGAATCCTCTTATATTGTCAGATGTTTTCCAATCAACCCCCTTGTCCCCTAAGGTTAAAATAATTTGAAATCGAGGTGGTTCAATGTCTAAGCCATGTTTCATGTTTATGTAGCGTCCTAGTTCATAACTATTGTCCTTTTCCCTCCTCGTCATTTTATTCTATCACTGAAATAGATTATTAAGACCGTTGTCCCCTTTTGCAGGCAGTGAACCCTCAGTACTTTCGGTATTTTAAACTTCACGTCATTTGAAGTTCATCAGTTCTATTCTGGAGAAAATGTTGGGTGGCAGCAATGGCAATGCCTTGCTTCCCGTTTTCCTGGATGAAAATCGAACTCAGTATCAAACTAATGCTTCTGGTCAGCTGCAGTTATTTGGAAATTGTGAGTTGTTGTTCTGTTTCTAAGAAACCTGATTTCAACTTAATGCATTCCATACATTTTGTTGTTATGTCTAACCATAAAAAAATATTTGAATTACAATATAAGTCTGTTCAGTCTCTAGAAGTTTAATCAGAACTTGTTGAGTGTTCTTTATCAATTGTCTCATCTCTCATAGAATTCACAATTTATATTGTAGTGCCCGTTAGCTGTGGAATTGAGCCTGTAAATTATTTTGGAAACGAGCAAATGGCTCATATGCTTCGCCCTAACAAAAGAAGTAGGGAAATAGAAGATTTCTCAAGACAGCAGAAGCTTCAGATTTCCTTAAATTATAATGCCTGTCAAGATGATGCTGATCGTTCAGCAAGCATTAGAAACCCAAATGCTGTATCAACAGGTTTAAGGCTATCGTATGATGATGATGAACACAACTCCTCTGTTACCACTGCAAGTGGAAGTATGACAGCTGCACCTTCCATCATCTTTTCGCTTGGTGATAATATCAGAACTGAGATTGATAGACAAAAGGAAGAATTTGATCAATATATTAAAATCCAGGTACTTTTAACCTCCTCAGCTTCTAGGACTTCCTGCTCTTCTGGATTTTTGCAAGAGTGCATACTGCATAGGGGGATAAAACAAAATTAATTGATGATGAGATTTCCTGGAAGATGTTTCTGGTCAAAGTCTTTATAGCAATCTATTCACACGATGCTTGCTAAATGGGGTGCTCTTGTTAAAGTATCCTTCAATGTGGTTGATTCAATTGCGGCTGATGTTTTAAATTTTCCATTTGCCTTGCGAACAGGAAGAACATTTGGCAAAGGGAATTAGGGACATGAAGCAGAGACATATGGCTTCCTTTCTTAATGCGATTGAGAAAGGCATCGGAAAGAAACTACACGAGAAAGATATTGAAATTGAGAACATGAACCGTAAAAATAGGGAACTAGTGGAGAGAATAAAGCAGGTTGCCACTGAAGCCCAGAATTGGCATTGCAGAGCAAAGTACAATGAATCAGTGGTAAATGTCCTGAAAAACAATCTACAGCATGCTATCTCGCAGGGTGCCGACCAAGCGAAGGAAGGATTTGGAGACAGTGAAGTGGATGATGCAGCCTCACACATTGATCCAAACAATTACCTTAGTGTTCCGGGTGGGGCTATGAAATCTTCAACCAGGAATTTACCACCTTTCAAGGAGCACATGATTTGCAGAGCATGCAAGGCTAAAGAGGTCTCCATCCTACTCATGCCTTGTAGACATTTATGTTTATGTAAAGACTGCGATGGACTTGTAAATGTCTGTCCTGTCTGTCAGTTGATCAAAACGGCTGGTATCCAAGTGTACTTGTCCTAAATTAAAATCTTACCAAAGCAGAAAAGAAATTCTTGTTATCCTTCTGTATGTATGATATATACCTGCATTTTTTTTTTATAAATCACTTAAGTGAACATTGAAGTTACTCGTAAAATTGAGTTTGAATTGAAAACCTGGGGTATCCCCTGTTT

mRNA sequence

CTCTCTCTCTCTCTCCTATAATTCTTCATCTTCATCTTCTTCTTCTTCTTCTTCTTCGTCGGTTACCACTCCAAAAGCCTTCATCCGTCTCTAGATCCTATTCTTCCTCCTTCATCATTCTCCTGCCCTTTTCATTCTACGTATTCCTATGCATATCTTCAAGGATCTGGAGATGATATCTGTTCTAATCTGAAACTGCCTGAATGACTTCTTCTTGAGGCAGTGAACCCTCAGTACTTTCGGTATTTTAAACTTCACGTCATTTGAAGTTCATCAGTTCTATTCTGGAGAAAATGTTGGGTGGCAGCAATGGCAATGCCTTGCTTCCCGTTTTCCTGGATGAAAATCGAACTCAGTATCAAACTAATGCTTCTGGTCAGCTGCAGTTATTTGGAAATTTGCCCGTTAGCTGTGGAATTGAGCCTGTAAATTATTTTGGAAACGAGCAAATGGCTCATATGCTTCGCCCTAACAAAAGAAGTAGGGAAATAGAAGATTTCTCAAGACAGCAGAAGCTTCAGATTTCCTTAAATTATAATGCCTGTCAAGATGATGCTGATCGTTCAGCAAGCATTAGAAACCCAAATGCTGTATCAACAGGTTTAAGGCTATCGTATGATGATGATGAACACAACTCCTCTGTTACCACTGCAAGTGGAAGTATGACAGCTGCACCTTCCATCATCTTTTCGCTTGGTGATAATATCAGAACTGAGATTGATAGACAAAAGGAAGAATTTGATCAATATATTAAAATCCAGGAAGAACATTTGGCAAAGGGAATTAGGGACATGAAGCAGAGACATATGGCTTCCTTTCTTAATGCGATTGAGAAAGGCATCGGAAAGAAACTACACGAGAAAGATATTGAAATTGAGAACATGAACCGTAAAAATAGGGAACTAGTGGAGAGAATAAAGCAGGTTGCCACTGAAGCCCAGAATTGGCATTGCAGAGCAAAGTACAATGAATCAGTGGTAAATGTCCTGAAAAACAATCTACAGCATGCTATCTCGCAGGGTGCCGACCAAGCGAAGGAAGGATTTGGAGACAGTGAAGTGGATGATGCAGCCTCACACATTGATCCAAACAATTACCTTAGTGTTCCGGGTGGGGCTATGAAATCTTCAACCAGGAATTTACCACCTTTCAAGGAGCACATGATTTGCAGAGCATGCAAGGCTAAAGAGGTCTCCATCCTACTCATGCCTTGTAGACATTTATGTTTATGTAAAGACTGCGATGGACTTGTAAATGTCTGTCCTGTCTGTCAGTTGATCAAAACGGCTGGTATCCAAGTGTACTTGTCCTAAATTAAAATCTTACCAAAGCAGAAAAGAAATTCTTGTTATCCTTCTGTATGTATGATATATACCTGCATTTTTTTTTTATAAATCACTTAAGTGAACATTGAAGTTACTCGTAAAATTGAGTTTGAATTGAAAACCTGGGGTATCCCCTGTTT

Coding sequence (CDS)

ATGTTGGGTGGCAGCAATGGCAATGCCTTGCTTCCCGTTTTCCTGGATGAAAATCGAACTCAGTATCAAACTAATGCTTCTGGTCAGCTGCAGTTATTTGGAAATTTGCCCGTTAGCTGTGGAATTGAGCCTGTAAATTATTTTGGAAACGAGCAAATGGCTCATATGCTTCGCCCTAACAAAAGAAGTAGGGAAATAGAAGATTTCTCAAGACAGCAGAAGCTTCAGATTTCCTTAAATTATAATGCCTGTCAAGATGATGCTGATCGTTCAGCAAGCATTAGAAACCCAAATGCTGTATCAACAGGTTTAAGGCTATCGTATGATGATGATGAACACAACTCCTCTGTTACCACTGCAAGTGGAAGTATGACAGCTGCACCTTCCATCATCTTTTCGCTTGGTGATAATATCAGAACTGAGATTGATAGACAAAAGGAAGAATTTGATCAATATATTAAAATCCAGGAAGAACATTTGGCAAAGGGAATTAGGGACATGAAGCAGAGACATATGGCTTCCTTTCTTAATGCGATTGAGAAAGGCATCGGAAAGAAACTACACGAGAAAGATATTGAAATTGAGAACATGAACCGTAAAAATAGGGAACTAGTGGAGAGAATAAAGCAGGTTGCCACTGAAGCCCAGAATTGGCATTGCAGAGCAAAGTACAATGAATCAGTGGTAAATGTCCTGAAAAACAATCTACAGCATGCTATCTCGCAGGGTGCCGACCAAGCGAAGGAAGGATTTGGAGACAGTGAAGTGGATGATGCAGCCTCACACATTGATCCAAACAATTACCTTAGTGTTCCGGGTGGGGCTATGAAATCTTCAACCAGGAATTTACCACCTTTCAAGGAGCACATGATTTGCAGAGCATGCAAGGCTAAAGAGGTCTCCATCCTACTCATGCCTTGTAGACATTTATGTTTATGTAAAGACTGCGATGGACTTGTAAATGTCTGTCCTGTCTGTCAGTTGATCAAAACGGCTGGTATCCAAGTGTACTTGTCCTAA

Protein sequence

MLGGSNGNALLPVFLDENRTQYQTNASGQLQLFGNLPVSCGIEPVNYFGNEQMAHMLRPNKRSREIEDFSRQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTASGSMTAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMASFLNAIEKGIGKKLHEKDIEIENMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAISQGADQAKEGFGDSEVDDAASHIDPNNYLSVPGGAMKSSTRNLPPFKEHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS
Homology
BLAST of Lcy01g010060 vs. ExPASy Swiss-Prot
Match: F4IDI6 (Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=BRG2 PE=1 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 1.2e-21
Identity = 75/243 (30.86%), Postives = 125/243 (51.44%), Query Frame = 0

Query: 115 SSVTTASGSMTAAPSIIFSLGD--NIRTEIDRQKEEFDQYIKI---QEEHLAKGIRDMKQ 174
           SS    + + T   ++   LG   +I + +++Q+ E D+++ +   Q E +   I + ++
Sbjct: 120 SSSCVNAATTTTTTTLFSFLGQDIDISSHMNQQQHEIDRFVSLHLYQMERVKYEIEEKRK 179

Query: 175 RHMASFLNAIEKGIGKKLHEKDIEIENMNRKNRELVERIKQVATEAQNWHCRAKYNESVV 234
           R   + + AIE+G+ K+L  K+ E E + + N  L ER+K ++ E Q W   A+ NE+  
Sbjct: 180 RQARTIMEAIEQGLVKRLRVKEEERERIGKVNHALEERVKSLSIENQIWRDLAQTNEATA 239

Query: 235 NVLKNNLQHAISQGADQAKEGFG----DSEVDDAASHIDPNNYLSVPGGAMKSSTRNLPP 294
           N L+ NL+H ++Q  D ++ G G     +E DDA S        S  GG  ++  R +  
Sbjct: 240 NHLRTNLEHVLAQVKDVSR-GAGLEKNMNEEDDAESCCGS----SCGGGGEETVRRRVGL 299

Query: 295 FKE---------HMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQV 340
            +E           +CR C  +E  +LL+PCRHLCLC  C   V+ CP+C   K A + V
Sbjct: 300 EREAQDKAERRRRRMCRNCGEEESCVLLLPCRHLCLCGVCGSSVHTCPICTSPKNASVHV 357

BLAST of Lcy01g010060 vs. ExPASy Swiss-Prot
Match: Q9LDD1 (Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=3702 GN=BRG3 PE=1 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.1e-19
Identity = 64/211 (30.33%), Postives = 106/211 (50.24%), Query Frame = 0

Query: 134 LGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMASFLNAIEKGIGKKLHEKDIE 193
           LG ++ + + +   + D+ I    E +   I + ++      + A+E+G+ K L  KD E
Sbjct: 126 LGQDLSSNVQQHHFDIDRLISNHVERMRMEIEEKRKTQGRRIVEAVEQGLMKTLRAKDDE 185

Query: 194 IENMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAISQGADQAKEGFGD 253
           I ++ + N  L E++K +  E Q W   A+ NE+ VN L++NLQ  ++   ++ +     
Sbjct: 186 INHIGKLNLFLEEKVKSLCVENQIWRDVAQSNEATVNALRSNLQQVLA-AVERNRWEEPP 245

Query: 254 SEVDDAASHIDPNNYLSVPGGAMK-----SSTRNLPPFKEHMICRACKAKEVSILLMPCR 313
           +  DDA S    N+         K       T+ +      M CR+C   E S+LL+PCR
Sbjct: 246 TVADDAQSCCGSNDEGDSEEERWKLAGEAQDTKKMCRVGMSM-CRSCGKGEASVLLLPCR 305

Query: 314 HLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           H+CLC  C   +N CP+C+  KTA + V LS
Sbjct: 306 HMCLCSVCGSSLNTCPICKSPKTASLHVNLS 334

BLAST of Lcy01g010060 vs. ExPASy Swiss-Prot
Match: O81851 (E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 2.3e-17
Identity = 73/249 (29.32%), Postives = 114/249 (45.78%), Query Frame = 0

Query: 100 VSTGLRLSYDDDEHNSSVTTASGSM-TAAPSII-FSLGDNIRTEIDRQKEEFDQYIKIQE 159
           +ST  +  +   + N+ V   S +  +++PS+I   L   I+   ++Q+ E D+++  Q 
Sbjct: 75  LSTKRQRDFQFSDSNAPVKRRSVAFDSSSPSLINVELVSQIQ---NQQQSEIDRFVAQQT 134

Query: 160 EHLAKGIRDMKQRHMASFLNAIEKGIGKKLHEKDIEIENMNRKNRELVERIKQVATEAQN 219
           E L   I   +Q       +A++  I KKL EKD EI  +   N  L ER+K +  E Q 
Sbjct: 135 EKLRIEIEARQQTQTRMLASAVQNVIAKKLKEKDDEIVRIRNLNWVLQERVKSLYVENQI 194

Query: 220 WHCRAKYNESVVNVLKNNLQHAISQ-----GADQAKEGFGDSEVDDAASHIDPNNYLSVP 279
           W   A+ NE+  N L+ NL   ++Q      A    E   +S               +V 
Sbjct: 195 WRDIAQTNEANANTLRTNLDQVLAQLETFPTASAVVEDDAESSCGSCCGDGGGEAVTAVG 254

Query: 280 GGAMKSSTRNLPPFKEHMICRACKAKEVSILLMPCRHLCLCKDCDG--LVNVCPVCQLIK 339
           GG                 C+ C  +E S+L++PCRHLCLC  C G  L+  CPVC ++ 
Sbjct: 255 GG-----------------CKRCGEREASVLVLPCRHLCLCTVCGGSALLRTCPVCDMVM 303

BLAST of Lcy01g010060 vs. ExPASy Swiss-Prot
Match: Q9FHE4 (BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG1 PE=1 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 6.7e-17
Identity = 73/234 (31.20%), Postives = 108/234 (46.15%), Query Frame = 0

Query: 110 DDEHNSSVTTASGSMTAAPSIIFSLGD-NIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMK 169
           D   +S    AS    A      SL D  + ++I +Q  E D+++  Q E L   +   +
Sbjct: 73  DSTFDSDALIASQKRRAIAFSPASLIDAELVSQIQQQNSEIDRFVAQQTETLRIELEARQ 132

Query: 170 QRHMASFLNAIEKGIGKKLHEKDIEIENMNRKNRELVERIKQVATEAQNWHCRAKYNESV 229
           +       +A++  I KKL  KD EI  M + N  L ER+K +  E Q W   A+ NE+ 
Sbjct: 133 RTQTRMLASAVQNAILKKLKAKDEEIIRMGKLNWVLQERVKNLYVENQIWRDLAQTNEAT 192

Query: 230 VNVLKNNLQHAISQGAD-QAKEGFGDSEVDDAASHIDPNNYLSVPGGAMKSSTRNLPPFK 289
            N L++NL+  ++Q  D  A       E DDA S        S  GG + +         
Sbjct: 193 ANNLRSNLEQVLAQVDDLDAFRRPLVEEADDAESSCG-----SCDGGDVTAVVNG----- 252

Query: 290 EHMICRACKAKEVSILLMPCRHLCLCKDC--DGLVNVCPVCQLIKTAGIQVYLS 340
               C+ C     S+L++PCRHLCLC  C    L+  CPVC ++ TA + V +S
Sbjct: 253 ---GCKRCGELTASVLVLPCRHLCLCTVCGSSALLRTCPVCDMVMTASVHVNMS 293

BLAST of Lcy01g010060 vs. ExPASy TrEMBL
Match: A0A6J1BT85 (BOI-related E3 ubiquitin-protein ligase 1 OS=Momordica charantia OX=3673 GN=LOC111005133 PE=4 SV=1)

HSP 1 Score: 646.4 bits (1666), Expect = 6.9e-182
Identity = 320/339 (94.40%), Postives = 330/339 (97.35%), Query Frame = 0

Query: 1   MLGGSNGNALLPVFLDENRTQYQTNASGQLQLFGNLPVSCGIEPVNYFGNEQMAHMLRPN 60
           MLGG NGNALLP+FLDENRTQYQ+NASGQLQLFGN PVSCGIEPVNYFG+EQMAHMLRPN
Sbjct: 1   MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPN 60

Query: 61  KRSREIEDFSRQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSRE+EDFSRQQKLQISLNYNAC DDADRSA+IRNPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSMTAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMASFLNAIE 180
           SGS+TAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRH ASFLNAIE
Sbjct: 121 SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIE 180

Query: 181 KGIGKKLHEKDIEIENMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240
           KGIG+KLHEKD+EIENMNRKNRELVERIKQVATEAQNWH RAKYNESVVNVLKNNLQHAI
Sbjct: 181 KGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAI 240

Query: 241 SQGADQAKEGFGDSEVDDAASHIDPNNYLSVPGGAMKSSTRNLPPFKEHMICRACKAKEV 300
           SQG DQAKEGFGDSEVDDAASH+DPNNYLSVPGG +KS TRNLP FKEHMICRACKAKEV
Sbjct: 241 SQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS
Sbjct: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 339

BLAST of Lcy01g010060 vs. ExPASy TrEMBL
Match: A0A6J1KC06 (BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111492498 PE=4 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 2.9e-180
Identity = 319/339 (94.10%), Postives = 330/339 (97.35%), Query Frame = 0

Query: 1   MLGGSNGNALLPVFLDENRTQYQTNASGQLQLFGNLPVSCGIEPVNYFGNEQMAHMLRPN 60
           MLGG+NGN LLPVF DENR QYQTNASGQLQLFG++PVSCGIEPVNYFG+EQ+AHMLRPN
Sbjct: 1   MLGGNNGNGLLPVFQDENRNQYQTNASGQLQLFGSVPVSCGIEPVNYFGSEQLAHMLRPN 60

Query: 61  KRSREIEDFSRQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSREIEDFS QQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREIEDFSWQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSMTAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMASFLNAIE 180
           SGSM AAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMASFL+AIE
Sbjct: 121 SGSMAAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMASFLSAIE 180

Query: 181 KGIGKKLHEKDIEIENMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240
           KGIGKKLHEKD+EIE+MNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI
Sbjct: 181 KGIGKKLHEKDVEIESMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240

Query: 241 SQGADQAKEGFGDSEVDDAASHIDPNNYLSVPGGAMKSSTRNLPPFKEHMICRACKAKEV 300
           SQGADQAKEGFGDSEVDDAASHIDPNNYL+V GG MKSST NLP FKEHMIC+ACKAKEV
Sbjct: 241 SQGADQAKEGFGDSEVDDAASHIDPNNYLNVHGGTMKSSTSNLPGFKEHMICKACKAKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           SILLMPCRHLCLC+DCDGLVNVCPVCQLIKTAGIQVYLS
Sbjct: 301 SILLMPCRHLCLCRDCDGLVNVCPVCQLIKTAGIQVYLS 339

BLAST of Lcy01g010060 vs. ExPASy TrEMBL
Match: A0A6J1HCD7 (BOI-related E3 ubiquitin-protein ligase 1 OS=Cucurbita moschata OX=3662 GN=LOC111462352 PE=4 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 2.4e-179
Identity = 317/339 (93.51%), Postives = 329/339 (97.05%), Query Frame = 0

Query: 1   MLGGSNGNALLPVFLDENRTQYQTNASGQLQLFGNLPVSCGIEPVNYFGNEQMAHMLRPN 60
           MLGG+NGN LLPVF DENR QYQTNASGQLQLFG++PVSCGIEPVNYFG+EQ+AHMLRPN
Sbjct: 1   MLGGNNGNGLLPVFQDENRNQYQTNASGQLQLFGSVPVSCGIEPVNYFGSEQLAHMLRPN 60

Query: 61  KRSREIEDFSRQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSREIEDFS QQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREIEDFSWQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSMTAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMASFLNAIE 180
           SGSM  APSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRH+ASFL+AIE
Sbjct: 121 SGSMATAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHVASFLSAIE 180

Query: 181 KGIGKKLHEKDIEIENMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240
           KGIGKKLHEKD+EIE+MNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI
Sbjct: 181 KGIGKKLHEKDVEIESMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240

Query: 241 SQGADQAKEGFGDSEVDDAASHIDPNNYLSVPGGAMKSSTRNLPPFKEHMICRACKAKEV 300
           SQGADQAKEGFGDSEVDDAASHIDPNNYL+V GG MKSST NLP FKEHMIC+ACKAKEV
Sbjct: 241 SQGADQAKEGFGDSEVDDAASHIDPNNYLNVHGGTMKSSTSNLPGFKEHMICKACKAKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           SILLMPCRHLCLC+DCDGLVNVCPVCQLIKTAGIQVYLS
Sbjct: 301 SILLMPCRHLCLCRDCDGLVNVCPVCQLIKTAGIQVYLS 339

BLAST of Lcy01g010060 vs. ExPASy TrEMBL
Match: A0A6J1GH18 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454110 PE=4 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 2.5e-176
Identity = 313/339 (92.33%), Postives = 325/339 (95.87%), Query Frame = 0

Query: 1   MLGGSNGNALLPVFLDENRTQYQTNASGQLQLFGNLPVSCGIEPVNYFGNEQMAHMLRPN 60
           MLGG NGNA+LPVFLDENRTQYQTNASGQLQLFGNLPVSCGIEPVNYFGNEQMAHMLRPN
Sbjct: 1   MLGGDNGNAMLPVFLDENRTQYQTNASGQLQLFGNLPVSCGIEPVNYFGNEQMAHMLRPN 60

Query: 61  KRSREIEDFSRQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSREIEDFSRQQKLQISL+YNACQDDADR ASI NPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREIEDFSRQQKLQISLDYNACQDDADRPASIINPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSMTAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMASFLNAIE 180
           SGSMTAAPSIIFSLGDN+R EIDRQKEE DQYIKIQEEHLAKGIR+MKQRHMASF+NAIE
Sbjct: 121 SGSMTAAPSIIFSLGDNLRIEIDRQKEELDQYIKIQEEHLAKGIREMKQRHMASFVNAIE 180

Query: 181 KGIGKKLHEKDIEIENMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240
           KGIGKKLHEKD+EIENMN KNRELVERIKQV TEAQNW CRAKYNESVVNVLK+NLQH I
Sbjct: 181 KGIGKKLHEKDVEIENMNCKNRELVERIKQVGTEAQNWQCRAKYNESVVNVLKSNLQHVI 240

Query: 241 SQGADQAKEGFGDSEVDDAASHIDPNNYLSVPGGAMKSSTRNLPPFKEHMICRACKAKEV 300
           SQGADQAKEGFGDSEVDDAASH+D NNYLSVPGG M+S+TRNL  FKE MICRACKAKEV
Sbjct: 241 SQGADQAKEGFGDSEVDDAASHVDSNNYLSVPGGTMRSTTRNLAGFKERMICRACKAKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           SILLMPCRHLCLCKDC+GLV+VCPVCQLIKTAGIQVYLS
Sbjct: 301 SILLMPCRHLCLCKDCNGLVSVCPVCQLIKTAGIQVYLS 339

BLAST of Lcy01g010060 vs. ExPASy TrEMBL
Match: A0A6J1KPW7 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496531 PE=4 SV=1)

HSP 1 Score: 625.9 bits (1613), Expect = 9.6e-176
Identity = 313/339 (92.33%), Postives = 323/339 (95.28%), Query Frame = 0

Query: 1   MLGGSNGNALLPVFLDENRTQYQTNASGQLQLFGNLPVSCGIEPVNYFGNEQMAHMLRPN 60
           MLGG NGNA+LPVFLDENRTQYQTNASGQLQLFGNLPVSCGIEPVNYFGNEQMAHMLRPN
Sbjct: 1   MLGGDNGNAMLPVFLDENRTQYQTNASGQLQLFGNLPVSCGIEPVNYFGNEQMAHMLRPN 60

Query: 61  KRSREIEDFSRQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSREIEDFSRQQKLQISL+YNACQDDADR ASI NPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREIEDFSRQQKLQISLDYNACQDDADRPASIINPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSMTAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMASFLNAIE 180
           SGSMTAAPSIIFSLGDN+R EIDRQKEE DQYIKIQEEHLAKGIR+MKQRHMASF+NAIE
Sbjct: 121 SGSMTAAPSIIFSLGDNLRIEIDRQKEELDQYIKIQEEHLAKGIREMKQRHMASFVNAIE 180

Query: 181 KGIGKKLHEKDIEIENMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240
           KGIGKKLHEKD+EIENMN KNRELVERIKQV TEAQNW CRAKYNESVVNVLK+NLQH I
Sbjct: 181 KGIGKKLHEKDVEIENMNCKNRELVERIKQVGTEAQNWQCRAKYNESVVNVLKSNLQHVI 240

Query: 241 SQGADQAKEGFGDSEVDDAASHIDPNNYLSVPGGAMKSSTRNLPPFKEHMICRACKAKEV 300
           SQGADQAKEGFGDSEVDDAASHID NNYLSVPGG M+SSTRNL  FKE MICRACK KEV
Sbjct: 241 SQGADQAKEGFGDSEVDDAASHIDSNNYLSVPGGTMRSSTRNLAGFKERMICRACKEKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           SILLMPCRHLCLCKDC+GL +VCPVCQLIKTAGIQVYLS
Sbjct: 301 SILLMPCRHLCLCKDCNGLASVCPVCQLIKTAGIQVYLS 339

BLAST of Lcy01g010060 vs. NCBI nr
Match: XP_022132217.1 (BOI-related E3 ubiquitin-protein ligase 1 [Momordica charantia])

HSP 1 Score: 646.4 bits (1666), Expect = 1.4e-181
Identity = 320/339 (94.40%), Postives = 330/339 (97.35%), Query Frame = 0

Query: 1   MLGGSNGNALLPVFLDENRTQYQTNASGQLQLFGNLPVSCGIEPVNYFGNEQMAHMLRPN 60
           MLGG NGNALLP+FLDENRTQYQ+NASGQLQLFGN PVSCGIEPVNYFG+EQMAHMLRPN
Sbjct: 1   MLGGDNGNALLPIFLDENRTQYQSNASGQLQLFGNFPVSCGIEPVNYFGSEQMAHMLRPN 60

Query: 61  KRSREIEDFSRQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSRE+EDFSRQQKLQISLNYNAC DDADRSA+IRNPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREVEDFSRQQKLQISLNYNACPDDADRSANIRNPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSMTAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMASFLNAIE 180
           SGS+TAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRH ASFLNAIE
Sbjct: 121 SGSITAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHTASFLNAIE 180

Query: 181 KGIGKKLHEKDIEIENMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240
           KGIG+KLHEKD+EIENMNRKNRELVERIKQVATEAQNWH RAKYNESVVNVLKNNLQHAI
Sbjct: 181 KGIGRKLHEKDVEIENMNRKNRELVERIKQVATEAQNWHSRAKYNESVVNVLKNNLQHAI 240

Query: 241 SQGADQAKEGFGDSEVDDAASHIDPNNYLSVPGGAMKSSTRNLPPFKEHMICRACKAKEV 300
           SQG DQAKEGFGDSEVDDAASH+DPNNYLSVPGG +KS TRNLP FKEHMICRACKAKEV
Sbjct: 241 SQGGDQAKEGFGDSEVDDAASHVDPNNYLSVPGGNVKSCTRNLPGFKEHMICRACKAKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS
Sbjct: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 339

BLAST of Lcy01g010060 vs. NCBI nr
Match: XP_022997618.1 (BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita maxima] >XP_022997619.1 BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita maxima] >XP_023545645.1 BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita pepo subsp. pepo] >XP_023545646.1 BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita pepo subsp. pepo] >XP_023545647.1 BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 641.0 bits (1652), Expect = 5.9e-180
Identity = 319/339 (94.10%), Postives = 330/339 (97.35%), Query Frame = 0

Query: 1   MLGGSNGNALLPVFLDENRTQYQTNASGQLQLFGNLPVSCGIEPVNYFGNEQMAHMLRPN 60
           MLGG+NGN LLPVF DENR QYQTNASGQLQLFG++PVSCGIEPVNYFG+EQ+AHMLRPN
Sbjct: 1   MLGGNNGNGLLPVFQDENRNQYQTNASGQLQLFGSVPVSCGIEPVNYFGSEQLAHMLRPN 60

Query: 61  KRSREIEDFSRQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSREIEDFS QQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREIEDFSWQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSMTAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMASFLNAIE 180
           SGSM AAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMASFL+AIE
Sbjct: 121 SGSMAAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMASFLSAIE 180

Query: 181 KGIGKKLHEKDIEIENMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240
           KGIGKKLHEKD+EIE+MNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI
Sbjct: 181 KGIGKKLHEKDVEIESMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240

Query: 241 SQGADQAKEGFGDSEVDDAASHIDPNNYLSVPGGAMKSSTRNLPPFKEHMICRACKAKEV 300
           SQGADQAKEGFGDSEVDDAASHIDPNNYL+V GG MKSST NLP FKEHMIC+ACKAKEV
Sbjct: 241 SQGADQAKEGFGDSEVDDAASHIDPNNYLNVHGGTMKSSTSNLPGFKEHMICKACKAKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           SILLMPCRHLCLC+DCDGLVNVCPVCQLIKTAGIQVYLS
Sbjct: 301 SILLMPCRHLCLCRDCDGLVNVCPVCQLIKTAGIQVYLS 339

BLAST of Lcy01g010060 vs. NCBI nr
Match: XP_038884634.1 (probable BOI-related E3 ubiquitin-protein ligase 2 [Benincasa hispida])

HSP 1 Score: 641.0 bits (1652), Expect = 5.9e-180
Identity = 321/339 (94.69%), Postives = 330/339 (97.35%), Query Frame = 0

Query: 1   MLGGSNGNALLPVFLDENRTQYQTNASGQLQLFGNLPVSCGIEPVNYFGNEQMAHMLRPN 60
           MLGG+NGN LLPVFLDENRTQYQTNASGQLQLFGNLPVSCGIEPVNYFGNEQ+ HMLRPN
Sbjct: 1   MLGGNNGNGLLPVFLDENRTQYQTNASGQLQLFGNLPVSCGIEPVNYFGNEQIVHMLRPN 60

Query: 61  KRSREIEDFSRQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSREIEDFSRQQKLQISLNY ACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREIEDFSRQQKLQISLNY-ACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSMTAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMASFLNAIE 180
           SGSMTAAPSII SLGDNIRTEIDRQKEEFDQYIK+QEEHLAKGIRDMKQRHMASFL+AIE
Sbjct: 121 SGSMTAAPSIILSLGDNIRTEIDRQKEEFDQYIKLQEEHLAKGIRDMKQRHMASFLSAIE 180

Query: 181 KGIGKKLHEKDIEIENMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240
           KGIGKKLHEKD+EIE+MNRKNRELVERIK VATEAQNWHCRAKYNESVVNVLKNNLQHAI
Sbjct: 181 KGIGKKLHEKDVEIESMNRKNRELVERIKHVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240

Query: 241 SQGADQAKEGFGDSEVDDAASHIDPNNYLSVPGGAMKSSTRNLPPFKEHMICRACKAKEV 300
           SQGADQAKEGFGDSEVDDAAS+IDPNNYLSVPGG +KSST NLP FKEHMICRACKAKEV
Sbjct: 241 SQGADQAKEGFGDSEVDDAASNIDPNNYLSVPGGTIKSSTSNLPGFKEHMICRACKAKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           SILLMPCRHLCLCKDCDGLVNVCPVC LIKTAGIQV+LS
Sbjct: 301 SILLMPCRHLCLCKDCDGLVNVCPVCHLIKTAGIQVFLS 338

BLAST of Lcy01g010060 vs. NCBI nr
Match: KAG6598221.1 (putative BOI-related E3 ubiquitin-protein ligase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 639.4 bits (1648), Expect = 1.7e-179
Identity = 318/339 (93.81%), Postives = 329/339 (97.05%), Query Frame = 0

Query: 1   MLGGSNGNALLPVFLDENRTQYQTNASGQLQLFGNLPVSCGIEPVNYFGNEQMAHMLRPN 60
           MLGG+NGN LLPVF DENR QYQTNASGQLQLFG++PVSCGIEPVNYFG+EQ+AHMLRPN
Sbjct: 1   MLGGNNGNGLLPVFQDENRNQYQTNASGQLQLFGSVPVSCGIEPVNYFGSEQLAHMLRPN 60

Query: 61  KRSREIEDFSRQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSREIEDFS QQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREIEDFSWQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSMTAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMASFLNAIE 180
           SGSM  APSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMASFL+AIE
Sbjct: 121 SGSMATAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMASFLSAIE 180

Query: 181 KGIGKKLHEKDIEIENMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240
           KGIGKKLHEKD+EIE+MNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI
Sbjct: 181 KGIGKKLHEKDVEIESMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240

Query: 241 SQGADQAKEGFGDSEVDDAASHIDPNNYLSVPGGAMKSSTRNLPPFKEHMICRACKAKEV 300
           SQGADQAKEGFGDSEVDDAASHIDPNNYL+V GG MKSST NLP FKEHMIC+ACKAKEV
Sbjct: 241 SQGADQAKEGFGDSEVDDAASHIDPNNYLNVHGGTMKSSTSNLPGFKEHMICKACKAKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           SILLMPCRHLCLC+DCDGLVNVCPVCQLIKTAGIQVYLS
Sbjct: 301 SILLMPCRHLCLCRDCDGLVNVCPVCQLIKTAGIQVYLS 339

BLAST of Lcy01g010060 vs. NCBI nr
Match: XP_022961638.1 (BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita moschata] >XP_022961639.1 BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita moschata] >XP_022961640.1 BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita moschata])

HSP 1 Score: 637.9 bits (1644), Expect = 5.0e-179
Identity = 317/339 (93.51%), Postives = 329/339 (97.05%), Query Frame = 0

Query: 1   MLGGSNGNALLPVFLDENRTQYQTNASGQLQLFGNLPVSCGIEPVNYFGNEQMAHMLRPN 60
           MLGG+NGN LLPVF DENR QYQTNASGQLQLFG++PVSCGIEPVNYFG+EQ+AHMLRPN
Sbjct: 1   MLGGNNGNGLLPVFQDENRNQYQTNASGQLQLFGSVPVSCGIEPVNYFGSEQLAHMLRPN 60

Query: 61  KRSREIEDFSRQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA 120
           KRSREIEDFS QQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA
Sbjct: 61  KRSREIEDFSWQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNSSVTTA 120

Query: 121 SGSMTAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMASFLNAIE 180
           SGSM  APSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRH+ASFL+AIE
Sbjct: 121 SGSMATAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHVASFLSAIE 180

Query: 181 KGIGKKLHEKDIEIENMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240
           KGIGKKLHEKD+EIE+MNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI
Sbjct: 181 KGIGKKLHEKDVEIESMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNNLQHAI 240

Query: 241 SQGADQAKEGFGDSEVDDAASHIDPNNYLSVPGGAMKSSTRNLPPFKEHMICRACKAKEV 300
           SQGADQAKEGFGDSEVDDAASHIDPNNYL+V GG MKSST NLP FKEHMIC+ACKAKEV
Sbjct: 241 SQGADQAKEGFGDSEVDDAASHIDPNNYLNVHGGTMKSSTSNLPGFKEHMICKACKAKEV 300

Query: 301 SILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           SILLMPCRHLCLC+DCDGLVNVCPVCQLIKTAGIQVYLS
Sbjct: 301 SILLMPCRHLCLCRDCDGLVNVCPVCQLIKTAGIQVYLS 339

BLAST of Lcy01g010060 vs. TAIR 10
Match: AT1G10650.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 377.9 bits (969), Expect = 8.7e-105
Identity = 202/352 (57.39%), Postives = 250/352 (71.02%), Query Frame = 0

Query: 1   MLGGSNGNALLPVFLDENRTQYQTN-ASGQLQLFGNLPVSCGIEPVNYFGNEQMAHMLRP 60
           ML G+NGN   PVF++EN+ QYQTN  S QL L GN+   C ++PVNYF N+ +  M+RP
Sbjct: 1   MLSGNNGNTAPPVFMNENQLQYQTNLRSNQLHLLGNMGGGCTVDPVNYFANDNLVPMIRP 60

Query: 61  NKRSREIEDFS-----RQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHN 120
           NKR RE E  S     +QQKLQ+SLNYN   ++      +   N VSTGLRLSYDDDEHN
Sbjct: 61  NKRGREAESISNNIQRQQQKLQMSLNYN--YNNTSVREEVPKENLVSTGLRLSYDDDEHN 120

Query: 121 SSVTTASGSMTAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMAS 180
           SSVT+ASGS+ AA  I  SL D++R ++ RQK+EFDQ+IKIQ   +AKG+RDMKQRH+AS
Sbjct: 121 SSVTSASGSILAASPIFQSLDDSLRIDLHRQKDEFDQFIKIQAAQMAKGVRDMKQRHIAS 180

Query: 181 FLNAIEKGIGKKLHEKDIEIENMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKN 240
           FL  +EKG+ KKL EKD EI +MN+KN+ELVERIKQVA EAQNWH RAKYNESVVNVLK 
Sbjct: 181 FLTTLEKGVSKKLQEKDHEINDMNKKNKELVERIKQVAMEAQNWHYRAKYNESVVNVLKA 240

Query: 241 NLQHAISQ------GADQAKEGFGDSEVDDAA-SHIDPNNYLSVPGGAMKSSTRNLPPFK 300
           NLQ A+S        ADQ KEGFGDSE+DDAA S+IDPNN           +  N     
Sbjct: 241 NLQQAMSHNNSVIAAADQGKEGFGDSEIDDAASSYIDPNN-----------NNNNNMGIH 300

Query: 301 EHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
           + M C+ C  KEVS+L++PCRHL LCK+CD    +CPVC+ +K++ +QV+ S
Sbjct: 301 QRMRCKMCNVKEVSVLIVPCRHLSLCKECDVFTKICPVCKSLKSSCVQVFFS 339

BLAST of Lcy01g010060 vs. TAIR 10
Match: AT1G60610.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 338.2 bits (866), Expect = 7.7e-93
Identity = 187/354 (52.82%), Postives = 241/354 (68.08%), Query Frame = 0

Query: 1   MLGGSNGNALLPVFLDENRTQYQTNAS-GQLQLFGNLPVSCGIEPVNYFGNEQMAHMLRP 60
           MLGG+N N +  V +++++ +YQ+N S  QL L G +   C I+PVNYF N+ +A M+R 
Sbjct: 1   MLGGNNDNPVPQVLMNDSQFRYQSNTSLNQLHLLGTMRAGCTIDPVNYFANDNLAPMMRL 60

Query: 61  NK-RSREIED---FSRQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNS 120
           N  R RE E+     RQQKLQISLNYN   ++      +   N VSTGLRLSYDDDE NS
Sbjct: 61  NSTRGRETENNNIMQRQQKLQISLNYNYNNNNTVVQDEVPKQNLVSTGLRLSYDDDERNS 120

Query: 121 SVTTASGSMTAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMASF 180
           SVT+A+GS+T    +  SLGDNIR +++RQ +E DQ+IK + + +AKG+RD+KQRH+ SF
Sbjct: 121 SVTSANGSITT--PVYQSLGDNIRLDLNRQNDELDQFIKFRADQMAKGVRDIKQRHVTSF 180

Query: 181 LNAIEKGIGKKLHEKDIEIENMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNN 240
           + A+EK + KKL EKD EIE+MN+KNRELV++IKQVA EAQNWH +AKYNESVVN LK N
Sbjct: 181 VTALEKDVSKKLQEKDHEIESMNKKNRELVDKIKQVAVEAQNWHYKAKYNESVVNALKVN 240

Query: 241 LQHAISQGAD------------QAKEGFGDSEVDDAASHIDPNNYLSVPGGAMKSSTRNL 300
           LQ  +S G D            Q KEGFGDSE+DD A+     NYL++PG  M S+    
Sbjct: 241 LQQVMSHGNDNNAVGGGVADHHQMKEGFGDSEIDDEAASY---NYLNIPG--MPST---- 300

Query: 301 PPFKEHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVY 338
                 M C+ C  K VS+LL+PCRHL LCKDCD    VCPVCQ +KT+ +QV+
Sbjct: 301 -----GMRCKLCNVKNVSVLLVPCRHLSLCKDCDVFTGVCPVCQSLKTSSVQVF 338

BLAST of Lcy01g010060 vs. TAIR 10
Match: AT1G60610.2 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 338.2 bits (866), Expect = 7.7e-93
Identity = 187/354 (52.82%), Postives = 241/354 (68.08%), Query Frame = 0

Query: 1   MLGGSNGNALLPVFLDENRTQYQTNAS-GQLQLFGNLPVSCGIEPVNYFGNEQMAHMLRP 60
           MLGG+N N +  V +++++ +YQ+N S  QL L G +   C I+PVNYF N+ +A M+R 
Sbjct: 1   MLGGNNDNPVPQVLMNDSQFRYQSNTSLNQLHLLGTMRAGCTIDPVNYFANDNLAPMMRL 60

Query: 61  NK-RSREIED---FSRQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNS 120
           N  R RE E+     RQQKLQISLNYN   ++      +   N VSTGLRLSYDDDE NS
Sbjct: 61  NSTRGRETENNNIMQRQQKLQISLNYNYNNNNTVVQDEVPKQNLVSTGLRLSYDDDERNS 120

Query: 121 SVTTASGSMTAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMASF 180
           SVT+A+GS+T    +  SLGDNIR +++RQ +E DQ+IK + + +AKG+RD+KQRH+ SF
Sbjct: 121 SVTSANGSITT--PVYQSLGDNIRLDLNRQNDELDQFIKFRADQMAKGVRDIKQRHVTSF 180

Query: 181 LNAIEKGIGKKLHEKDIEIENMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNN 240
           + A+EK + KKL EKD EIE+MN+KNRELV++IKQVA EAQNWH +AKYNESVVN LK N
Sbjct: 181 VTALEKDVSKKLQEKDHEIESMNKKNRELVDKIKQVAVEAQNWHYKAKYNESVVNALKVN 240

Query: 241 LQHAISQGAD------------QAKEGFGDSEVDDAASHIDPNNYLSVPGGAMKSSTRNL 300
           LQ  +S G D            Q KEGFGDSE+DD A+     NYL++PG  M S+    
Sbjct: 241 LQQVMSHGNDNNAVGGGVADHHQMKEGFGDSEIDDEAASY---NYLNIPG--MPST---- 300

Query: 301 PPFKEHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVY 338
                 M C+ C  K VS+LL+PCRHL LCKDCD    VCPVCQ +KT+ +QV+
Sbjct: 301 -----GMRCKLCNVKNVSVLLVPCRHLSLCKDCDVFTGVCPVCQSLKTSSVQVF 338

BLAST of Lcy01g010060 vs. TAIR 10
Match: AT1G60610.3 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 338.2 bits (866), Expect = 7.7e-93
Identity = 187/354 (52.82%), Postives = 241/354 (68.08%), Query Frame = 0

Query: 1   MLGGSNGNALLPVFLDENRTQYQTNAS-GQLQLFGNLPVSCGIEPVNYFGNEQMAHMLRP 60
           MLGG+N N +  V +++++ +YQ+N S  QL L G +   C I+PVNYF N+ +A M+R 
Sbjct: 1   MLGGNNDNPVPQVLMNDSQFRYQSNTSLNQLHLLGTMRAGCTIDPVNYFANDNLAPMMRL 60

Query: 61  NK-RSREIED---FSRQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDDDEHNS 120
           N  R RE E+     RQQKLQISLNYN   ++      +   N VSTGLRLSYDDDE NS
Sbjct: 61  NSTRGRETENNNIMQRQQKLQISLNYNYNNNNTVVQDEVPKQNLVSTGLRLSYDDDERNS 120

Query: 121 SVTTASGSMTAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQRHMASF 180
           SVT+A+GS+T    +  SLGDNIR +++RQ +E DQ+IK + + +AKG+RD+KQRH+ SF
Sbjct: 121 SVTSANGSITT--PVYQSLGDNIRLDLNRQNDELDQFIKFRADQMAKGVRDIKQRHVTSF 180

Query: 181 LNAIEKGIGKKLHEKDIEIENMNRKNRELVERIKQVATEAQNWHCRAKYNESVVNVLKNN 240
           + A+EK + KKL EKD EIE+MN+KNRELV++IKQVA EAQNWH +AKYNESVVN LK N
Sbjct: 181 VTALEKDVSKKLQEKDHEIESMNKKNRELVDKIKQVAVEAQNWHYKAKYNESVVNALKVN 240

Query: 241 LQHAISQGAD------------QAKEGFGDSEVDDAASHIDPNNYLSVPGGAMKSSTRNL 300
           LQ  +S G D            Q KEGFGDSE+DD A+     NYL++PG  M S+    
Sbjct: 241 LQQVMSHGNDNNAVGGGVADHHQMKEGFGDSEIDDEAASY---NYLNIPG--MPST---- 300

Query: 301 PPFKEHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVY 338
                 M C+ C  K VS+LL+PCRHL LCKDCD    VCPVCQ +KT+ +QV+
Sbjct: 301 -----GMRCKLCNVKNVSVLLVPCRHLSLCKDCDVFTGVCPVCQSLKTSSVQVF 338

BLAST of Lcy01g010060 vs. TAIR 10
Match: AT1G10650.2 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 317.0 bits (811), Expect = 1.8e-86
Identity = 173/296 (58.45%), Postives = 212/296 (71.62%), Query Frame = 0

Query: 56  MLRPNKRSREIEDFS-----RQQKLQISLNYNACQDDADRSASIRNPNAVSTGLRLSYDD 115
           M+RPNKR RE E  S     +QQKLQ+SLNYN   ++      +   N VSTGLRLSYDD
Sbjct: 1   MIRPNKRGREAESISNNIQRQQQKLQMSLNYN--YNNTSVREEVPKENLVSTGLRLSYDD 60

Query: 116 DEHNSSVTTASGSMTAAPSIIFSLGDNIRTEIDRQKEEFDQYIKIQEEHLAKGIRDMKQR 175
           DEHNSSVT+ASGS+ AA  I  SL D++R ++ RQK+EFDQ+IKIQ   +AKG+RDMKQR
Sbjct: 61  DEHNSSVTSASGSILAASPIFQSLDDSLRIDLHRQKDEFDQFIKIQAAQMAKGVRDMKQR 120

Query: 176 HMASFLNAIEKGIGKKLHEKDIEIENMNRKNRELVERIKQVATEAQNWHCRAKYNESVVN 235
           H+ASFL  +EKG+ KKL EKD EI +MN+KN+ELVERIKQVA EAQNWH RAKYNESVVN
Sbjct: 121 HIASFLTTLEKGVSKKLQEKDHEINDMNKKNKELVERIKQVAMEAQNWHYRAKYNESVVN 180

Query: 236 VLKNNLQHAISQ------GADQAKEGFGDSEVDDAA-SHIDPNNYLSVPGGAMKSSTRNL 295
           VLK NLQ A+S        ADQ KEGFGDSE+DDAA S+IDPNN           +  N 
Sbjct: 181 VLKANLQQAMSHNNSVIAAADQGKEGFGDSEIDDAASSYIDPNN-----------NNNNN 240

Query: 296 PPFKEHMICRACKAKEVSILLMPCRHLCLCKDCDGLVNVCPVCQLIKTAGIQVYLS 340
               + M C+ C  KEVS+L++PCRHL LCK+CD    +CPVC+ +K++ +QV+ S
Sbjct: 241 MGIHQRMRCKMCNVKEVSVLIVPCRHLSLCKECDVFTKICPVCKSLKSSCVQVFFS 283

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4IDI61.2e-2130.86Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=37... [more]
Q9LDD11.1e-1930.33Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=37... [more]
O818512.3e-1729.32E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1[more]
Q9FHE46.7e-1731.20BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG... [more]
Match NameE-valueIdentityDescription
A0A6J1BT856.9e-18294.40BOI-related E3 ubiquitin-protein ligase 1 OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A6J1KC062.9e-18094.10BOI-related E3 ubiquitin-protein ligase 1-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1HCD72.4e-17993.51BOI-related E3 ubiquitin-protein ligase 1 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1GH182.5e-17692.33probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucurbita mosch... [more]
A0A6J1KPW79.6e-17692.33probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucurbita maxim... [more]
Match NameE-valueIdentityDescription
XP_022132217.11.4e-18194.40BOI-related E3 ubiquitin-protein ligase 1 [Momordica charantia][more]
XP_022997618.15.9e-18094.10BOI-related E3 ubiquitin-protein ligase 1-like [Cucurbita maxima] >XP_022997619.... [more]
XP_038884634.15.9e-18094.69probable BOI-related E3 ubiquitin-protein ligase 2 [Benincasa hispida][more]
KAG6598221.11.7e-17993.81putative BOI-related E3 ubiquitin-protein ligase 2, partial [Cucurbita argyrospe... [more]
XP_022961638.15.0e-17993.51BOI-related E3 ubiquitin-protein ligase 1 [Cucurbita moschata] >XP_022961639.1 B... [more]
Match NameE-valueIdentityDescription
AT1G10650.18.7e-10557.39SBP (S-ribonuclease binding protein) family protein [more]
AT1G60610.17.7e-9352.82SBP (S-ribonuclease binding protein) family protein [more]
AT1G60610.27.7e-9352.82SBP (S-ribonuclease binding protein) family protein [more]
AT1G60610.37.7e-9352.82SBP (S-ribonuclease binding protein) family protein [more]
AT1G10650.21.8e-8658.45SBP (S-ribonuclease binding protein) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 187..214
NoneNo IPR availablePFAMPF13920zf-C3HC4_3coord: 290..327
e-value: 4.8E-7
score: 29.5
NoneNo IPR availableGENE3D1.10.8.10coord: 86..157
e-value: 3.5E-11
score: 45.1
NoneNo IPR availableGENE3D1.10.1170.10coord: 74..330
e-value: 3.5E-11
score: 45.1
NoneNo IPR availablePANTHERPTHR42647:SF9S-RIBONUCLEASE BINDING PROTEIN SBP1-RELATEDcoord: 4..338
IPR011029Death-like domain superfamilyGENE3D1.10.533.10Death Domain, Fascoord: 158..300
e-value: 3.5E-11
score: 45.1
IPR017066E3 ubiquitin-protein ligase BOI-likePIRSFPIRSF036836SBP1_RNase_bindcoord: 1..339
e-value: 4.8E-99
score: 330.2
IPR017066E3 ubiquitin-protein ligase BOI-likePANTHERPTHR42647SBP (S-RIBONUCLEASE BINDING PROTEIN) FAMILY PROTEINcoord: 4..338
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 292..327
score: 8.963311

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy01g010060.1Lcy01g010060.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004842 ubiquitin-protein transferase activity