Lcy01g009590 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy01g009590
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	nicotianamine synthase-like
Location	Chr01: 28151959 .. 28152957 (-)
RNA-Seq Expression	Lcy01g009590
Synteny	Lcy01g009590

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCTCCACTCATCTCAAGGGTAACCATTTTGTTGAATACTTCCTCTGTATAGGTATCTTCCTATTCACTACTGTTGGATTATTTTTGACGACTGGAAGAGGAAAAGTTAAAAAAGTTTGAATTTTTCTCTCTTAGTTGTCAAAAATTTGACAGTGTGATATAGTAAGATTTCTATCCATATGTGTCACAACTAAATATTATTCTTAATTTCTCTCTCTCAATTGAATTCTTAGTTGCATGTGTAGAAGATATCTTCCTAACTACTGCTATTGAATTTTTGACAACTAAGTAAAAAGGTTCAAATTTCTCTCTCTAAATTGTCAAGAATTTAATGGTGGGGAGTAAGATTCCTACCTTACCCATCGGTGTCGGTCGTTAAATATAGTTCTTAATTTCATTCATTTATTTCTTACTAAACAATACTTGGTTGCATACTGATGTGTAGGTACTGTTTTCCACAACTTTGACATCGATTCGACTGCGAACTCCATAGCGGGTCGGTTGGTCAGCTCCGACCCGGACCTGTCGCGGCGGATGGTGTTTCACACGAAGGATGTAATGGAGGTGACGAAGGCATTGGGGGAGTACGAGGTGGTGTTTCTAGCAGCATTGGTGGAGTTGGAAGAGGAAGATAAGGCAAGGGTTTTGAGGCATTTAGGGAAGCATATGGCACCCGGGGCTTACTTGATGCTGAGGAGTGCACATGGGGCTAGGGATTTTTTGTACCCTGTTGTCGATGTTTGTGACGTTGAAGCTTCTGGATTTGAGATTCTCTCTGCGTTTCACCCAACTGACGAAGTCATAAACTCTGTTGTTATTGCAAGGAAGGTGTTCGATCTTATTCCAGAAGCCGAAGGAAACTCTGTTGAAGATGATGATAATGAGAAGCGAGGTGTTTTAGTTAACACCCCTATTTTGTTGGCTGAAAAATGCTCTGGGTTCAAAGGATTTGGTCCCATGATCGAGGAGCTTGCCATGGAAGAGCAACTCTCTTGA

mRNA sequence

ATGGCCTCCACTCATCTCAAGGGTACTGTTTTCCACAACTTTGACATCGATTCGACTGCGAACTCCATAGCGGGTCGGTTGGTCAGCTCCGACCCGGACCTGTCGCGGCGGATGGTGTTTCACACGAAGGATGTAATGGAGGTGACGAAGGCATTGGGGGAGTACGAGGTGGTGTTTCTAGCAGCATTGGTGGAGTTGGAAGAGGAAGATAAGGCAAGGGTTTTGAGGCATTTAGGGAAGCATATGGCACCCGGGGCTTACTTGATGCTGAGGAGTGCACATGGGGCTAGGGATTTTTTGTACCCTGTTGTCGATGTTTGTGACGTTGAAGCTTCTGGATTTGAGATTCTCTCTGCGTTTCACCCAACTGACGAAGTCATAAACTCTGTTGTTATTGCAAGGAAGGTGTTCGATCTTATTCCAGAAGCCGAAGGAAACTCTGTTGAAGATGATGATAATGAGAAGCGAGGTGTTTTAGTTAACACCCCTATTTTGTTGGCTGAAAAATGCTCTGGGTTCAAAGGATTTGGTCCCATGATCGAGGAGCTTGCCATGGAAGAGCAACTCTCTTGA

Coding sequence (CDS)

ATGGCCTCCACTCATCTCAAGGGTACTGTTTTCCACAACTTTGACATCGATTCGACTGCGAACTCCATAGCGGGTCGGTTGGTCAGCTCCGACCCGGACCTGTCGCGGCGGATGGTGTTTCACACGAAGGATGTAATGGAGGTGACGAAGGCATTGGGGGAGTACGAGGTGGTGTTTCTAGCAGCATTGGTGGAGTTGGAAGAGGAAGATAAGGCAAGGGTTTTGAGGCATTTAGGGAAGCATATGGCACCCGGGGCTTACTTGATGCTGAGGAGTGCACATGGGGCTAGGGATTTTTTGTACCCTGTTGTCGATGTTTGTGACGTTGAAGCTTCTGGATTTGAGATTCTCTCTGCGTTTCACCCAACTGACGAAGTCATAAACTCTGTTGTTATTGCAAGGAAGGTGTTCGATCTTATTCCAGAAGCCGAAGGAAACTCTGTTGAAGATGATGATAATGAGAAGCGAGGTGTTTTAGTTAACACCCCTATTTTGTTGGCTGAAAAATGCTCTGGGTTCAAAGGATTTGGTCCCATGATCGAGGAGCTTGCCATGGAAGAGCAACTCTCTTGA

Protein sequence

MASTHLKGTVFHNFDIDSTANSIAGRLVSSDPDLSRRMVFHTKDVMEVTKALGEYEVVFLAALVELEEEDKARVLRHLGKHMAPGAYLMLRSAHGARDFLYPVVDVCDVEASGFEILSAFHPTDEVINSVVIARKVFDLIPEAEGNSVEDDDNEKRGVLVNTPILLAEKCSGFKGFGPMIEELAMEEQLS

Homology

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BLAST of Lcy01g009590 vs. ExPASy Swiss-Prot
Match: Q9C7X5 (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 7.8e-47
Identity = 88/136 (64.71%), Postives = 117/136 (86.03%), Query Frame = 0


Query: 1   MASTHLKGTVFHNFDIDSTANSIAGRLVSSDPDLSRRMVFHTKDVMEVTKALGEYEVVFL 60
           +AS+HLK ++FHNFDID +AN +A RLVSSDPDLS+RM FHT D+M+VT++L  ++VVFL
Sbjct: 143 LASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFFHTVDIMDVTESLKGFDVVFL 202

Query: 61  AALVELEEEDKARVLRHLGKHMAPGAYLMLRSAHGARDFLYPVVDVCDVEASGFEILSAF 120
           AALV +++++K +V+ HL KHM+PGA LMLRSAHG R FLYP+V+ CD+E  GFE+LS +
Sbjct: 203 AALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFLYPIVEPCDLE--GFEVLSVY 262

Query: 121 HPTDEVINSVVIARKV 137
           HPTDEVINS+VI+RK+
Sbjct: 263 HPTDEVINSIVISRKL 276

[Close]

BLAST of Lcy01g009590 vs. ExPASy Swiss-Prot
Match: O80483 (Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 3.8e-46
Identity = 88/135 (65.19%), Postives = 114/135 (84.44%), Query Frame = 0


Query: 1   MASTHLKGTVFHNFDIDSTANSIAGRLVSSDPDLSRRMVFHTKDVMEVTKALGEYEVVFL 60
           +AS HLK T+FHNFDID +ANS+A  LVSSDPD+S+RM FHT D+M+VT++L  ++VVFL
Sbjct: 142 LASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMFFHTVDIMDVTESLKSFDVVFL 201

Query: 61  AALVELEEEDKARVLRHLGKHMAPGAYLMLRSAHGARDFLYPVVDVCDVEASGFEILSAF 120
           AALV + +E+K +V+ HL KHMAPGA LMLRSAHG R FLYP+V+ CD++  GFE+LS +
Sbjct: 202 AALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAFLYPIVEPCDLQ--GFEVLSIY 261

Query: 121 HPTDEVINSVVIARK 136
           HPTD+VINSVVI++K
Sbjct: 262 HPTDDVINSVVISKK 274

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BLAST of Lcy01g009590 vs. ExPASy Swiss-Prot
Match: Q9XGI7 (Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 1.0e-43
Identity = 88/136 (64.71%), Postives = 111/136 (81.62%), Query Frame = 0


Query: 1   MASTHLKGTVFHNFDIDSTANSIAGRLVSSDPDLSRRMVFHTKDVMEVTKALGEYEVVFL 60
           +A+ HLK T FHN+DID  AN +A  LV++DPD+S RM FHT DVM+VT AL +Y+VVFL
Sbjct: 140 LATKHLKTTCFHNYDIDVDANFMASALVAADPDMSSRMTFHTADVMDVTCALKDYDVVFL 199

Query: 61  AALVELEEEDKARVLRHLGKHMAPGAYLMLRSAHGARDFLYPVVDVCDVEASGFEILSAF 120
           AALV +++EDK +V+ HL K+M+PGA LMLRSAHGAR FLYPV+D  D+   GFE+LS +
Sbjct: 200 AALVGMDKEDKVKVVDHLAKYMSPGATLMLRSAHGARAFLYPVLDPRDLR--GFEVLSVY 259

Query: 121 HPTDEVINSVVIARKV 137
           HPTDEVINSV+IARK+
Sbjct: 260 HPTDEVINSVIIARKL 273

[Close]

BLAST of Lcy01g009590 vs. ExPASy Swiss-Prot
Match: Q9FF79 (Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 6.8e-43
Identity = 83/136 (61.03%), Postives = 107/136 (78.68%), Query Frame = 0


Query: 1   MASTHLKGTVFHNFDIDSTANSIAGRLVSSDPDLSRRMVFHTKDVMEVTKALGEYEVVFL 60
           +A  HL  T FHNFDIDS AN++A  LVS DPDLS+RM+FHT DV+  T+ L +Y+VVFL
Sbjct: 143 LAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKRMIFHTTDVLNATEGLDQYDVVFL 202

Query: 61  AALVELEEEDKARVLRHLGKHMAPGAYLMLRSAHGARDFLYPVVDVCDVEASGFEILSAF 120
           AALV +++E K + + HL KHMAPGA LMLRSAH  R FLYP+VD  D++  GF++L+ +
Sbjct: 203 AALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALRAFLYPIVDSSDLK--GFQLLTIY 262

Query: 121 HPTDEVINSVVIARKV 137
           HPTD+V+NSVVIARK+
Sbjct: 263 HPTDDVVNSVVIARKL 276

[Close]

BLAST of Lcy01g009590 vs. ExPASy Swiss-Prot
Match: Q9FKT9 (Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 8.9e-43
Identity = 82/136 (60.29%), Postives = 107/136 (78.68%), Query Frame = 0


Query: 1   MASTHLKGTVFHNFDIDSTANSIAGRLVSSDPDLSRRMVFHTKDVMEVTKALGEYEVVFL 60
           +A  HL  T FHNFDIDS AN++A  LVS D DLS+RM+FHT DV+   + L +Y+VVFL
Sbjct: 142 LAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRMIFHTTDVLNAKEGLDQYDVVFL 201

Query: 61  AALVELEEEDKARVLRHLGKHMAPGAYLMLRSAHGARDFLYPVVDVCDVEASGFEILSAF 120
           AALV +++E K + + HL KHMAPGA +MLRSAHG R FLYP+VD CD++  GFE+L+ +
Sbjct: 202 AALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAFLYPIVDSCDLK--GFEVLTIY 261

Query: 121 HPTDEVINSVVIARKV 137
           HP+D+V+NSVVIARK+
Sbjct: 262 HPSDDVVNSVVIARKL 275

[Close]

BLAST of Lcy01g009590 vs. ExPASy TrEMBL
Match: A0A6J1G3L4 (nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111450477 PE=3 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 6.5e-81
Identity = 156/190 (82.11%), Postives = 170/190 (89.47%), Query Frame = 0


Query: 1   MASTHLKGTVFHNFDIDSTANSIAGRLVSSDPDLSRRMVFHTKDVMEVTKALGEYEVVFL 60
           MASTHLKGT+FHNFDID  ANS+AG+LV SD DLS+RMVFHT DVMEVT+A GEYEVVFL
Sbjct: 137 MASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVFHTTDVMEVTEAFGEYEVVFL 196

Query: 61  AALVELEEEDKARVLRHLGKHMAPGAYLMLRSAHGARDFLYPVVDVCDVEASGFEILSAF 120
           AALV LEEE+K RVLRHLGKHMAPGAYLMLRSAHGAR FLYPVVDV DVEASGFEILS F
Sbjct: 197 AALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVVDVRDVEASGFEILSVF 256

Query: 121 HPTDEVINSVVIARKVFDLIPEAEGNSVEDDDNEKRGVLVNTPILLAEKCSGFKGFGPMI 180
           HPTDEVINSVVIARKV +L+P+ +  +  D D+EKR VLVNTPI+LAEKCSGFK FGPMI
Sbjct: 257 HPTDEVINSVVIARKVSNLVPDNDNYNDNDTDDEKRAVLVNTPIILAEKCSGFKDFGPMI 316

Query: 181 EELAMEEQLS 191
           EELA+EEQLS
Sbjct: 317 EELAIEEQLS 326

[Close]

BLAST of Lcy01g009590 vs. ExPASy TrEMBL
Match: A0A6J1KIA7 (nicotianamine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111493502 PE=3 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 2.1e-79
Identity = 153/190 (80.53%), Postives = 167/190 (87.89%), Query Frame = 0


Query: 1   MASTHLKGTVFHNFDIDSTANSIAGRLVSSDPDLSRRMVFHTKDVMEVTKALGEYEVVFL 60
           MASTHLKGT+FHNFDID  ANS+A RL+ SD DLS+RMVFHT DVMEVTKA GEYEVVFL
Sbjct: 167 MASTHLKGTIFHNFDIDPAANSMARRLIRSDSDLSKRMVFHTADVMEVTKAFGEYEVVFL 226

Query: 61  AALVELEEEDKARVLRHLGKHMAPGAYLMLRSAHGARDFLYPVVDVCDVEASGFEILSAF 120
           AALV LEEE+K RVLRHLGKHMAPGAYLMLRSAHGAR FLYPVVDV D+EASGFEILS F
Sbjct: 227 AALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVVDVSDIEASGFEILSVF 286

Query: 121 HPTDEVINSVVIARKVFDLIPEAEGNSVEDDDNEKRGVLVNTPILLAEKCSGFKGFGPMI 180
           HPTDEVINSVVIARKV DL+ +    +  + D+EKRGVLVNTPI+LAEKCSGFK FGPMI
Sbjct: 287 HPTDEVINSVVIARKVSDLVLDNNNYNDNNTDDEKRGVLVNTPIILAEKCSGFKDFGPMI 346

Query: 181 EELAMEEQLS 191
           +EL +EEQLS
Sbjct: 347 DELGIEEQLS 356

[Close]

BLAST of Lcy01g009590 vs. ExPASy TrEMBL
Match: A0A0A0LGI4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034530 PE=3 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 8.3e-68
Identity = 136/185 (73.51%), Postives = 158/185 (85.41%), Query Frame = 0


Query: 1   MASTHLKGTVFHNFDIDSTANSIAGRLVSSDPDLSRRMVFHTKDVMEVTKALGEYEVVFL 60
           +AS HLKGT+FHNFDID TAN++A +LV SDPDLS+RM+FHTKDVMEVTK L +YEVVFL
Sbjct: 147 LASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMIFHTKDVMEVTKGLKDYEVVFL 206

Query: 61  AALVELEEEDKARVLRHLGKHMAPGAYLMLRSAHGARDFLYPVVDVCDVEASGFEILSAF 120
           AALV L EE+K RVL+HLGKHMA G+YLMLRSAHGAR FLYPVVD+C VEASGFEILS F
Sbjct: 207 AALVGLGEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAFLYPVVDICTVEASGFEILSVF 266

Query: 121 HPTDEVINSVVIARK--VFDLIPEAEGNSVEDDDNEKRGVLVNT-PILLAEKCSGFKGFG 180
           HPTDEVINSVVIARK  +FD     + +  ++D++EK+G+LVNT  I+LAEKCSGF GF 
Sbjct: 267 HPTDEVINSVVIARKKVMFD-----QSSDEDEDEDEKQGILVNTSSIVLAEKCSGFNGFT 326

Query: 181 PMIEE 183
           PMIEE
Sbjct: 327 PMIEE 326

[Close]

BLAST of Lcy01g009590 vs. ExPASy TrEMBL
Match: A0A5D3BBH0 (Putative nicotianamine synthase 4 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001110 PE=3 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 2.4e-67
Identity = 136/185 (73.51%), Postives = 156/185 (84.32%), Query Frame = 0


Query: 1   MASTHLKGTVFHNFDIDSTANSIAGRLVSSDPDLSRRMVFHTKDVMEVTKALGEYEVVFL 60
           +AS HLKGT+FHNFDID TAN++A +LV SDPDLS+RM+FHTKDVMEVTK L +YEVVFL
Sbjct: 152 LASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMIFHTKDVMEVTKGLKDYEVVFL 211

Query: 61  AALVELEEEDKARVLRHLGKHMAPGAYLMLRSAHGARDFLYPVVDVCDVEASGFEILSAF 120
           AALV LEEE+K RVL+HLGKHMA G+YLMLRSAHGAR FLYPVVD+C VEASGFEILS F
Sbjct: 212 AALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAFLYPVVDICTVEASGFEILSVF 271

Query: 121 HPTDEVINSVVIARK--VFDLIPEAEGNSVEDDDNEKRGVLVNT-PILLAEKCSGFKGFG 180
           HPTDEVINSVVIARK  VFD     E    ++++ EK G+LV+T  I+LAEKCSGF GF 
Sbjct: 272 HPTDEVINSVVIARKKVVFDQ-SSVEDEDEDEEEEEKPGILVDTSSIVLAEKCSGFNGFT 331

Query: 181 PMIEE 183
           P+IEE
Sbjct: 332 PIIEE 335

[Close]

BLAST of Lcy01g009590 vs. ExPASy TrEMBL
Match: A0A1S3BXI8 (probable nicotianamine synthase 4 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103494174 PE=3 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 2.5e-56
Identity = 114/140 (81.43%), Postives = 126/140 (90.00%), Query Frame = 0


Query: 1   MASTHLKGTVFHNFDIDSTANSIAGRLVSSDPDLSRRMVFHTKDVMEVTKALGEYEVVFL 60
           +AS HLKGT+FHNFDID TAN++A +LV SDPDLS+RM+FHTKDVMEVTK L +YEVVFL
Sbjct: 152 LASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMIFHTKDVMEVTKGLKDYEVVFL 211

Query: 61  AALVELEEEDKARVLRHLGKHMAPGAYLMLRSAHGARDFLYPVVDVCDVEASGFEILSAF 120
           AALV LEEE+K RVL+HLGKHMA G+YLMLRSAHGAR FLYPVVD+C VEASGFEILS F
Sbjct: 212 AALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAFLYPVVDICTVEASGFEILSVF 271

Query: 121 HPTDEVINSVVIARK--VFD 139
           HPTDEVINSVVIARK  VFD
Sbjct: 272 HPTDEVINSVVIARKKVVFD 291

[Close]

BLAST of Lcy01g009590 vs. NCBI nr
Match: KAG7030119.1 (Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 316.2 bits (809), Expect = 1.9e-82
Identity = 160/190 (84.21%), Postives = 171/190 (90.00%), Query Frame = 0


Query: 1   MASTHLKGTVFHNFDIDSTANSIAGRLVSSDPDLSRRMVFHTKDVMEVTKALGEYEVVFL 60
           MASTHLKGT+FHNFDID  ANS+AGRLV SD DLS+RMVFHT DVMEVT+A GEYEVVFL
Sbjct: 137 MASTHLKGTIFHNFDIDPVANSMAGRLVCSDSDLSKRMVFHTADVMEVTEAFGEYEVVFL 196

Query: 61  AALVELEEEDKARVLRHLGKHMAPGAYLMLRSAHGARDFLYPVVDVCDVEASGFEILSAF 120
           AALV LEEE+K RVLRHLGKHMAPGAYLMLRSAHGAR FLYPVVDV DVEASGFEILS F
Sbjct: 197 AALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVVDVRDVEASGFEILSVF 256

Query: 121 HPTDEVINSVVIARKVFDLIPEAEGNSVEDDDNEKRGVLVNTPILLAEKCSGFKGFGPMI 180
           HPTDEVINSVVIARKV DLIP+ +  +  D D+EKRGVLVNTPI+LAEKCSGFK FGPMI
Sbjct: 257 HPTDEVINSVVIARKVSDLIPDNDNYNNNDTDDEKRGVLVNTPIILAEKCSGFKDFGPMI 316

Query: 181 EELAMEEQLS 191
           EELA+EEQLS
Sbjct: 317 EELAIEEQLS 326

[Close]

BLAST of Lcy01g009590 vs. NCBI nr
Match: KAG6599185.1 (Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 316.2 bits (809), Expect = 1.9e-82
Identity = 160/190 (84.21%), Postives = 171/190 (90.00%), Query Frame = 0


Query: 1   MASTHLKGTVFHNFDIDSTANSIAGRLVSSDPDLSRRMVFHTKDVMEVTKALGEYEVVFL 60
           MASTHLKGT+FHNFDID  ANS+AGRLV SD DLS+RMVFHT DVMEVT+A GEYEVVFL
Sbjct: 137 MASTHLKGTIFHNFDIDPVANSMAGRLVCSDSDLSKRMVFHTADVMEVTEAFGEYEVVFL 196

Query: 61  AALVELEEEDKARVLRHLGKHMAPGAYLMLRSAHGARDFLYPVVDVCDVEASGFEILSAF 120
           AALV LEEE+K RVLRHLGKHMAPGAYLMLRSAHGAR FLYPVVDV DVEASGFEILS F
Sbjct: 197 AALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVVDVRDVEASGFEILSVF 256

Query: 121 HPTDEVINSVVIARKVFDLIPEAEGNSVEDDDNEKRGVLVNTPILLAEKCSGFKGFGPMI 180
           HPTDEVINSVVIARKV DLIP+ +  +  D D+EKRGVLVNTPI+LAEKCSGFK FGPMI
Sbjct: 257 HPTDEVINSVVIARKVSDLIPDNDNYNNNDTDDEKRGVLVNTPIILAEKCSGFKDFGPMI 316

Query: 181 EELAMEEQLS 191
           EELA+EEQLS
Sbjct: 317 EELAIEEQLS 326

[Close]

BLAST of Lcy01g009590 vs. NCBI nr
Match: XP_022946411.1 (nicotianamine synthase-like [Cucurbita moschata])

HSP 1 Score: 310.1 bits (793), Expect = 1.3e-80
Identity = 156/190 (82.11%), Postives = 170/190 (89.47%), Query Frame = 0


Query: 1   MASTHLKGTVFHNFDIDSTANSIAGRLVSSDPDLSRRMVFHTKDVMEVTKALGEYEVVFL 60
           MASTHLKGT+FHNFDID  ANS+AG+LV SD DLS+RMVFHT DVMEVT+A GEYEVVFL
Sbjct: 137 MASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVFHTTDVMEVTEAFGEYEVVFL 196

Query: 61  AALVELEEEDKARVLRHLGKHMAPGAYLMLRSAHGARDFLYPVVDVCDVEASGFEILSAF 120
           AALV LEEE+K RVLRHLGKHMAPGAYLMLRSAHGAR FLYPVVDV DVEASGFEILS F
Sbjct: 197 AALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVVDVRDVEASGFEILSVF 256

Query: 121 HPTDEVINSVVIARKVFDLIPEAEGNSVEDDDNEKRGVLVNTPILLAEKCSGFKGFGPMI 180
           HPTDEVINSVVIARKV +L+P+ +  +  D D+EKR VLVNTPI+LAEKCSGFK FGPMI
Sbjct: 257 HPTDEVINSVVIARKVSNLVPDNDNYNDNDTDDEKRAVLVNTPIILAEKCSGFKDFGPMI 316

Query: 181 EELAMEEQLS 191
           EELA+EEQLS
Sbjct: 317 EELAIEEQLS 326

[Close]

BLAST of Lcy01g009590 vs. NCBI nr
Match: XP_023545298.1 (nicotianamine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 308.9 bits (790), Expect = 3.0e-80
Identity = 155/190 (81.58%), Postives = 169/190 (88.95%), Query Frame = 0


Query: 1   MASTHLKGTVFHNFDIDSTANSIAGRLVSSDPDLSRRMVFHTKDVMEVTKALGEYEVVFL 60
           MASTHLKGT+FHNFDID  AN++AG+LV SD DLS+RMVFHT DVMEVT+A GEYEVVFL
Sbjct: 137 MASTHLKGTIFHNFDIDPAANTMAGQLVCSDSDLSKRMVFHTADVMEVTEAFGEYEVVFL 196

Query: 61  AALVELEEEDKARVLRHLGKHMAPGAYLMLRSAHGARDFLYPVVDVCDVEASGFEILSAF 120
           AALV LEEE+K RVLRHLGKHMAPGAYLMLRSAHGAR FLYPVVDV DVEASGFEILS F
Sbjct: 197 AALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVVDVRDVEASGFEILSVF 256

Query: 121 HPTDEVINSVVIARKVFDLIPEAEGNSVEDDDNEKRGVLVNTPILLAEKCSGFKGFGPMI 180
           HPTDEVINSVVIARKV DL+P+ +  +  D D+EKR VLVN PI+LAEKCSGFK FGPMI
Sbjct: 257 HPTDEVINSVVIARKVTDLVPDNDNYNDNDTDDEKRAVLVNAPIILAEKCSGFKDFGPMI 316

Query: 181 EELAMEEQLS 191
           EELA+EEQLS
Sbjct: 317 EELAIEEQLS 326

[Close]

BLAST of Lcy01g009590 vs. NCBI nr
Match: XP_022998973.1 (nicotianamine synthase-like, partial [Cucurbita maxima])

HSP 1 Score: 305.1 bits (780), Expect = 4.3e-79
Identity = 153/190 (80.53%), Postives = 167/190 (87.89%), Query Frame = 0


Query: 1   MASTHLKGTVFHNFDIDSTANSIAGRLVSSDPDLSRRMVFHTKDVMEVTKALGEYEVVFL 60
           MASTHLKGT+FHNFDID  ANS+A RL+ SD DLS+RMVFHT DVMEVTKA GEYEVVFL
Sbjct: 167 MASTHLKGTIFHNFDIDPAANSMARRLIRSDSDLSKRMVFHTADVMEVTKAFGEYEVVFL 226

Query: 61  AALVELEEEDKARVLRHLGKHMAPGAYLMLRSAHGARDFLYPVVDVCDVEASGFEILSAF 120
           AALV LEEE+K RVLRHLGKHMAPGAYLMLRSAHGAR FLYPVVDV D+EASGFEILS F
Sbjct: 227 AALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVVDVSDIEASGFEILSVF 286

Query: 121 HPTDEVINSVVIARKVFDLIPEAEGNSVEDDDNEKRGVLVNTPILLAEKCSGFKGFGPMI 180
           HPTDEVINSVVIARKV DL+ +    +  + D+EKRGVLVNTPI+LAEKCSGFK FGPMI
Sbjct: 287 HPTDEVINSVVIARKVSDLVLDNNNYNDNNTDDEKRGVLVNTPIILAEKCSGFKDFGPMI 346

Query: 181 EELAMEEQLS 191
           +EL +EEQLS
Sbjct: 347 DELGIEEQLS 356

[Close]

BLAST of Lcy01g009590 vs. TAIR 10
Match: AT1G56430.1 (nicotianamine synthase 4 )

HSP 1 Score: 188.3 bits (477), Expect = 5.5e-48
Identity = 88/136 (64.71%), Postives = 117/136 (86.03%), Query Frame = 0


Query: 1   MASTHLKGTVFHNFDIDSTANSIAGRLVSSDPDLSRRMVFHTKDVMEVTKALGEYEVVFL 60
           +AS+HLK ++FHNFDID +AN +A RLVSSDPDLS+RM FHT D+M+VT++L  ++VVFL
Sbjct: 143 LASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFFHTVDIMDVTESLKGFDVVFL 202

Query: 61  AALVELEEEDKARVLRHLGKHMAPGAYLMLRSAHGARDFLYPVVDVCDVEASGFEILSAF 120
           AALV +++++K +V+ HL KHM+PGA LMLRSAHG R FLYP+V+ CD+E  GFE+LS +
Sbjct: 203 AALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFLYPIVEPCDLE--GFEVLSVY 262

Query: 121 HPTDEVINSVVIARKV 137
           HPTDEVINS+VI+RK+
Sbjct: 263 HPTDEVINSIVISRKL 276

[Close]

BLAST of Lcy01g009590 vs. TAIR 10
Match: AT1G09240.1 (nicotianamine synthase 3 )

HSP 1 Score: 186.0 bits (471), Expect = 2.7e-47
Identity = 88/135 (65.19%), Postives = 114/135 (84.44%), Query Frame = 0


Query: 1   MASTHLKGTVFHNFDIDSTANSIAGRLVSSDPDLSRRMVFHTKDVMEVTKALGEYEVVFL 60
           +AS HLK T+FHNFDID +ANS+A  LVSSDPD+S+RM FHT D+M+VT++L  ++VVFL
Sbjct: 142 LASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMFFHTVDIMDVTESLKSFDVVFL 201

Query: 61  AALVELEEEDKARVLRHLGKHMAPGAYLMLRSAHGARDFLYPVVDVCDVEASGFEILSAF 120
           AALV + +E+K +V+ HL KHMAPGA LMLRSAHG R FLYP+V+ CD++  GFE+LS +
Sbjct: 202 AALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAFLYPIVEPCDLQ--GFEVLSIY 261

Query: 121 HPTDEVINSVVIARK 136
           HPTD+VINSVVI++K
Sbjct: 262 HPTDDVINSVVISKK 274

[Close]

BLAST of Lcy01g009590 vs. TAIR 10
Match: AT5G04950.1 (nicotianamine synthase 1 )

HSP 1 Score: 175.3 bits (443), Expect = 4.8e-44
Identity = 83/136 (61.03%), Postives = 107/136 (78.68%), Query Frame = 0


Query: 1   MASTHLKGTVFHNFDIDSTANSIAGRLVSSDPDLSRRMVFHTKDVMEVTKALGEYEVVFL 60
           +A  HL  T FHNFDIDS AN++A  LVS DPDLS+RM+FHT DV+  T+ L +Y+VVFL
Sbjct: 143 LAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKRMIFHTTDVLNATEGLDQYDVVFL 202

Query: 61  AALVELEEEDKARVLRHLGKHMAPGAYLMLRSAHGARDFLYPVVDVCDVEASGFEILSAF 120
           AALV +++E K + + HL KHMAPGA LMLRSAH  R FLYP+VD  D++  GF++L+ +
Sbjct: 203 AALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALRAFLYPIVDSSDLK--GFQLLTIY 262

Query: 121 HPTDEVINSVVIARKV 137
           HPTD+V+NSVVIARK+
Sbjct: 263 HPTDDVVNSVVIARKL 276

[Close]

BLAST of Lcy01g009590 vs. TAIR 10
Match: AT5G56080.1 (nicotianamine synthase 2 )

HSP 1 Score: 174.9 bits (442), Expect = 6.3e-44
Identity = 82/136 (60.29%), Postives = 107/136 (78.68%), Query Frame = 0


Query: 1   MASTHLKGTVFHNFDIDSTANSIAGRLVSSDPDLSRRMVFHTKDVMEVTKALGEYEVVFL 60
           +A  HL  T FHNFDIDS AN++A  LVS D DLS+RM+FHT DV+   + L +Y+VVFL
Sbjct: 142 LAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRMIFHTTDVLNAKEGLDQYDVVFL 201

Query: 61  AALVELEEEDKARVLRHLGKHMAPGAYLMLRSAHGARDFLYPVVDVCDVEASGFEILSAF 120
           AALV +++E K + + HL KHMAPGA +MLRSAHG R FLYP+VD CD++  GFE+L+ +
Sbjct: 202 AALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAFLYPIVDSCDLK--GFEVLTIY 261

Query: 121 HPTDEVINSVVIARKV 137
           HP+D+V+NSVVIARK+
Sbjct: 262 HPSDDVVNSVVIARKL 275

[Close]

BLAST of Lcy01g009590 vs. TAIR 10
Match: AT4G26483.1 (nicotianamine synthases )

HSP 1 Score: 65.5 bits (158), Expect = 5.4e-11
Identity = 35/70 (50.00%), Postives = 48/70 (68.57%), Query Frame = 0


Query: 82  MAPGAYLMLRSAHGARDFLYPVVDVCDVEASGFEILSAFHP--TDEVINSVVIARKVFDL 141
           M PGA LMLRSA G R FLY  VD CD++  GFE+L  +HP  +D  +NSV++ARK+ D 
Sbjct: 1   MDPGALLMLRSARGLRSFLYVDVDPCDLK--GFEVLEIYHPSMSDGFVNSVMVARKLTDR 60

Query: 142 IPEAEGNSVE 150
           + + E + +E
Sbjct: 61  LIKYEWSRLE 68

The following BLAST results are available for this feature:

BLAST of Lcy01g009590 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C7X5	7.8e-47	64.71	Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 S...	[more]
O80483	3.8e-46	65.19	Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1	[more]
Q9XGI7	1.0e-43	64.71	Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1	[more]
Q9FF79	6.8e-43	61.03	Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1	[more]
Q9FKT9	8.9e-43	60.29	Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1	[more]

BLAST of Lcy01g009590 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G3L4	6.5e-81	82.11	nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111450477 PE=3 S...	[more]
A0A6J1KIA7	2.1e-79	80.53	nicotianamine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111493502 PE=3 SV=...	[more]
A0A0A0LGI4	8.3e-68	73.51	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034530 PE=3 SV=1	[more]
A0A5D3BBH0	2.4e-67	73.51	Putative nicotianamine synthase 4 isoform X2 OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A1S3BXI8	2.5e-56	81.43	probable nicotianamine synthase 4 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10349...	[more]

BLAST of Lcy01g009590 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7030119.1	1.9e-82	84.21	Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6599185.1	1.9e-82	84.21	Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022946411.1	1.3e-80	82.11	nicotianamine synthase-like [Cucurbita moschata]	[more]
XP_023545298.1	3.0e-80	81.58	nicotianamine synthase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022998973.1	4.3e-79	80.53	nicotianamine synthase-like, partial [Cucurbita maxima]	[more]

BLAST of Lcy01g009590 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G56430.1	5.5e-48	64.71	nicotianamine synthase 4	[more]
AT1G09240.1	2.7e-47	65.19	nicotianamine synthase 3	[more]
AT5G04950.1	4.8e-44	61.03	nicotianamine synthase 1	[more]
AT5G56080.1	6.3e-44	60.29	nicotianamine synthase 2	[more]
AT4G26483.1	5.4e-11	50.00	nicotianamine synthases	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 1..137 e-value: 1.9E-33 score: 117.7
IPR004298	Nicotianamine synthase	PFAM	PF03059	NAS	coord: 1..136 e-value: 7.6E-55 score: 186.2
IPR004298	Nicotianamine synthase	PANTHER	PTHR32266	NICOTIANAMINE SYNTHASE 3	coord: 1..140
IPR004298	Nicotianamine synthase	PROSITE	PS51142	NAS	coord: 1..140 score: 27.059153
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 33..98

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy01g009590.1	Lcy01g009590.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030418	nicotianamine biosynthetic process
molecular_function	GO:0030410	nicotianamine synthase activity