Lcy01g008530 (gene) Sponge gourd (P93075) v1

Overview
NameLcy01g008530
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptionnaringenin,2-oxoglutarate 3-dioxygenase-like
LocationChr01: 20096424 .. 20098438 (+)
RNA-Seq ExpressionLcy01g008530
SyntenyLcy01g008530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTGTTTGACGCATATAAATCCTCTTTTCTACCGCCCATTTTTACACAATCCACCTCGCCATGGCCACTTTCATCGAGGAGGAAAATGAAATCCCAGTCGTTTCTCTCGCCAGAATCGACGACGCCGCCGATAGCCATAGAGCGGCGGTGAAGAAGAAGATCGTGGAGGCGTGCGAGGTGTGGGGAATATTCCAGGTGGTGGACCATGGAGTTGACCCCAAACTTATATCCGACATGATTCGTCTCGCCAATGAGTTCTTTGCTTTTCCACGAGAACAGAAGCTTCAGTTCGACATGTCGGCCGGCAAGAAGGGCGGATTCATGATCCCCGGTCAACGAAAGGTATCTGCAACTGAAACGCTCTGTTTTACACACTAACACAAATCTTTGTCAAAATGTTATTTAAACTCAAAAAGGAAAACTTTTTTCTTTCATTATTTAATTTTTGACAGCTATTAGCATCCTGGCAATCACTGAGCCAATTTTTAACATTCACCCAATAGAAAAACATGTTAAAACCGTAAGGATTAATTAAAATAGATGATAAATTTTGTAGGGAGAAGCACAAGATTGGCGGGAAATTGTAATGTTCTTTACGTATCCGATCAGAAATCGGAACTACTCAGAGTGGCCAGACATGCCAGTCGGATGGATAGAGAAGACACAGAAATTCAGCGAGCAGATGATGGAATTGGCTTCTAAACTACTCCAAGTGTTGTCCGAAGCCATGGATTTGGACAAGGAAGCTTTAAAGAATGCCTGTGGAGAGCTTAACCAGAAGATTTTGGTCAATTTCTACCCCAAATGTTTGCACTCCGATCTTGATCGCGGACTCCGCCGTCACACGGATCCCGGAACCATTACCATTTTATTACAGGCACCCAGCTAACTCTCTTTAAAATATCCTTTTTGTTTTTGAACTGAGAATTAGGATCTCTATCCTTTTGAAGTACTATTTTAATTTATGAACTTTTATAATGCCTATTTTCATCCTCCAACTATATAAAAAAAATGAATATATATTTGGATTTTATTTTACCAAATGAAATAATTGGTACTTAATCTTAGACCTACCCCATGTAGTTTCAAACATGTGTTAATTCACATGTTGGTTCTTAGCTGGAAAGAAACACAATGATAAGCATCAAAATGATTCTTTTCTTTTTTTTTTTTTTTCCAATTTATGGGATTATAATAGACATTTTACTGGATTCTTCCCATGTCAAATCCTTATTAGGCTTACGAAAATGCATGATAATAAAAATATTGATATTTGGATGAAAATTAATTATATTTTATAATTACAATTTACAGGATCAAGTGAGTGGGCTGCAAGCTACCAAAGATGGGGGCAAGACATGGATGAACATCAAACCCATGGAAGGAGCTTTTGTAGTCAACATCGGAGACCATGGTCATGTATGTAATCTTCTTGTCTGTTTTGGCAAAGTCTATTAATTTCATTATAATAAACTTTGAAATTCATGGAATTGGAAGGCTATAATAATATAATTGAGAATGATTGGAAGTGAAAATGATGCAGTATCTTAGCAATGGGAGGTTCAAGAATGCTGATCACAGGGCATTGGCGAGCCCAAACAGCAACAGAATTTCGATAGCAGCGTTTCAGTACCCAGCACCAGAGGCCATTGTTTATCCATTGAAGGTTGGGGAAGGGGAGAAGCCTTTGCTTGACACGCCCATTAGCTTCCAAGAAATGTACAACAAGAAAATGAGCAACGAACCTACGCTTGCAGTTCTTAACAATAAAGACCCCCACAACCTCAAACTCTCTGCTTAATTTTAATTTCTAGTGTTTAAGAAAAAACACTTTTGTTTAGGCTTCTTATCCTTTTATTAAGCCCTTCATCTTTTATGTATTCTACTCACTGTTCTTGTTCGCGTGTTTTTCTACTTTGTTCCAAGTATTGTGTCCTGTTATAACATTTGCAATTTCTGTTTTCTACTAGTTTTACATATTTCTAATTTTAAGTTTAATCTTAGATATGATTTGT

mRNA sequence

ATTGTTTGACGCATATAAATCCTCTTTTCTACCGCCCATTTTTACACAATCCACCTCGCCATGGCCACTTTCATCGAGGAGGAAAATGAAATCCCAGTCGTTTCTCTCGCCAGAATCGACGACGCCGCCGATAGCCATAGAGCGGCGGTGAAGAAGAAGATCGTGGAGGCGTGCGAGGTGTGGGGAATATTCCAGGTGGTGGACCATGGAGTTGACCCCAAACTTATATCCGACATGATTCGTCTCGCCAATGAGTTCTTTGCTTTTCCACGAGAACAGAAGCTTCAGTTCGACATGTCGGCCGGCAAGAAGGGCGGATTCATGATCCCCGGTCAACGAAAGGGAGAAGCACAAGATTGGCGGGAAATTGTAATGTTCTTTACGTATCCGATCAGAAATCGGAACTACTCAGAGTGGCCAGACATGCCAGTCGGATGGATAGAGAAGACACAGAAATTCAGCGAGCAGATGATGGAATTGGCTTCTAAACTACTCCAAGTGTTGTCCGAAGCCATGGATTTGGACAAGGAAGCTTTAAAGAATGCCTGTGGAGAGCTTAACCAGAAGATTTTGGTCAATTTCTACCCCAAATGTTTGCACTCCGATCTTGATCGCGGACTCCGCCGTCACACGGATCCCGGAACCATTACCATTTTATTACAGGATCAAGTGAGTGGGCTGCAAGCTACCAAAGATGGGGGCAAGACATGGATGAACATCAAACCCATGGAAGGAGCTTTTGTAGTCAACATCGGAGACCATGGTCATTATCTTAGCAATGGGAGGTTCAAGAATGCTGATCACAGGGCATTGGCGAGCCCAAACAGCAACAGAATTTCGATAGCAGCGTTTCAGTACCCAGCACCAGAGGCCATTGTTTATCCATTGAAGGTTGGGGAAGGGGAGAAGCCTTTGCTTGACACGCCCATTAGCTTCCAAGAAATGTACAACAAGAAAATGAGCAACGAACCTACGCTTGCAGTTCTTAACAATAAAGACCCCCACAACCTCAAACTCTCTGCTTAATTTTAATTTCTAGTGTTTAAGAAAAAACACTTTTGTTTAGGCTTCTTATCCTTTTATTAAGCCCTTCATCTTTTATGTATTCTACTCACTGTTCTTGTTCGCGTGTTTTTCTACTTTGTTCCAAGTATTGTGTCCTGTTATAACATTTGCAATTTCTGTTTTCTACTAGTTTTACATATTTCTAATTTTAAGTTTAATCTTAGATATGATTTGT

Coding sequence (CDS)

ATGGCCACTTTCATCGAGGAGGAAAATGAAATCCCAGTCGTTTCTCTCGCCAGAATCGACGACGCCGCCGATAGCCATAGAGCGGCGGTGAAGAAGAAGATCGTGGAGGCGTGCGAGGTGTGGGGAATATTCCAGGTGGTGGACCATGGAGTTGACCCCAAACTTATATCCGACATGATTCGTCTCGCCAATGAGTTCTTTGCTTTTCCACGAGAACAGAAGCTTCAGTTCGACATGTCGGCCGGCAAGAAGGGCGGATTCATGATCCCCGGTCAACGAAAGGGAGAAGCACAAGATTGGCGGGAAATTGTAATGTTCTTTACGTATCCGATCAGAAATCGGAACTACTCAGAGTGGCCAGACATGCCAGTCGGATGGATAGAGAAGACACAGAAATTCAGCGAGCAGATGATGGAATTGGCTTCTAAACTACTCCAAGTGTTGTCCGAAGCCATGGATTTGGACAAGGAAGCTTTAAAGAATGCCTGTGGAGAGCTTAACCAGAAGATTTTGGTCAATTTCTACCCCAAATGTTTGCACTCCGATCTTGATCGCGGACTCCGCCGTCACACGGATCCCGGAACCATTACCATTTTATTACAGGATCAAGTGAGTGGGCTGCAAGCTACCAAAGATGGGGGCAAGACATGGATGAACATCAAACCCATGGAAGGAGCTTTTGTAGTCAACATCGGAGACCATGGTCATTATCTTAGCAATGGGAGGTTCAAGAATGCTGATCACAGGGCATTGGCGAGCCCAAACAGCAACAGAATTTCGATAGCAGCGTTTCAGTACCCAGCACCAGAGGCCATTGTTTATCCATTGAAGGTTGGGGAAGGGGAGAAGCCTTTGCTTGACACGCCCATTAGCTTCCAAGAAATGTACAACAAGAAAATGAGCAACGAACCTACGCTTGCAGTTCTTAACAATAAAGACCCCCACAACCTCAAACTCTCTGCTTAA

Protein sequence

MATFIEEENEIPVVSLARIDDAADSHRAAVKKKIVEACEVWGIFQVVDHGVDPKLISDMIRLANEFFAFPREQKLQFDMSAGKKGGFMIPGQRKGEAQDWREIVMFFTYPIRNRNYSEWPDMPVGWIEKTQKFSEQMMELASKLLQVLSEAMDLDKEALKNACGELNQKILVNFYPKCLHSDLDRGLRRHTDPGTITILLQDQVSGLQATKDGGKTWMNIKPMEGAFVVNIGDHGHYLSNGRFKNADHRALASPNSNRISIAAFQYPAPEAIVYPLKVGEGEKPLLDTPISFQEMYNKKMSNEPTLAVLNNKDPHNLKLSA
Homology
BLAST of Lcy01g008530 vs. ExPASy Swiss-Prot
Match: Q9S818 (Naringenin,2-oxoglutarate 3-dioxygenase OS=Arabidopsis thaliana OX=3702 GN=F3H PE=1 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 4.6e-116
Identity = 196/302 (64.90%), Postives = 247/302 (81.79%), Query Frame = 0

Query: 9   NEIPVVSLARIDDAADSHRAAVKKKIVEACEVWGIFQVVDHGVDPKLISDMIRLANEFFA 68
           +EIPV+SLA IDD  D  R  + ++IVEACE WGIFQVVDHGVD  L++DM RLA +FFA
Sbjct: 36  DEIPVISLAGIDD-VDGKRGEICRQIVEACENWGIFQVVDHGVDTNLVADMTRLARDFFA 95

Query: 69  FPREQKLQFDMSAGKKGGFMIPGQRKGEA-QDWREIVMFFTYPIRNRNYSEWPDMPVGWI 128
            P E KL+FDMS GKKGGF++    +GEA QDWREIV +F+YP+RNR+YS WPD P GW+
Sbjct: 96  LPPEDKLRFDMSGGKKGGFIVSSHLQGEAVQDWREIVTYFSYPVRNRDYSRWPDKPEGWV 155

Query: 129 EKTQKFSEQMMELASKLLQVLSEAMDLDKEALKNACGELNQKILVNFYPKCLHSDLDRGL 188
           + T+++SE++M LA KLL+VLSEAM L+KE+L NAC +++QKI+VN+YPKC   DL  GL
Sbjct: 156 KVTEEYSERLMSLACKLLEVLSEAMGLEKESLTNACVDMDQKIVVNYYPKCPQPDLTLGL 215

Query: 189 RRHTDPGTITILLQDQVSGLQATKDGGKTWMNIKPMEGAFVVNIGDHGHYLSNGRFKNAD 248
           +RHTDPGTIT+LLQDQV GLQAT+D GKTW+ ++P+EGAFVVN+GDHGH+LSNGRFKNAD
Sbjct: 216 KRHTDPGTITLLLQDQVGGLQATRDNGKTWITVQPVEGAFVVNLGDHGHFLSNGRFKNAD 275

Query: 249 HRALASPNSNRISIAAFQYPAPEAIVYPLKVGEGEKPLLDTPISFQEMYNKKMSNEPTLA 308
           H+A+ + NS+R+SIA FQ PAP+A VYPLKV EGEK +L+ PI+F EMY +KM  +  LA
Sbjct: 276 HQAVVNSNSSRLSIATFQNPAPDATVYPLKVREGEKAILEEPITFAEMYKRKMGRDLELA 335

Query: 309 VL 310
            L
Sbjct: 336 RL 336

BLAST of Lcy01g008530 vs. ExPASy Swiss-Prot
Match: Q05965 (Naringenin,2-oxoglutarate 3-dioxygenase (Fragment) OS=Matthiola incana OX=3724 GN=FHT PE=2 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 4.6e-116
Identity = 201/319 (63.01%), Postives = 254/319 (79.62%), Query Frame = 0

Query: 6   EEENEIPVVSLARIDDAADSHRAAVKKKIVEACEVWGIFQVVDHGVDPKLISDMIRLANE 65
           E  +EIPV+SLA IDD  D  R  + ++IVEACE WGIFQVVDHGVD  L++DM RLA +
Sbjct: 32  EFSDEIPVISLAGIDD-VDGKRGEICREIVEACENWGIFQVVDHGVDTSLVADMTRLARD 91

Query: 66  FFAFPREQKLQFDMSAGKKGGFMIPGQRKGEA-QDWREIVMFFTYPIRNRNYSEWPDMPV 125
           FFA P E+KL+FDMS GKKGGF++    +GEA QDWREIV +F+YP+RNR+YS WPD P 
Sbjct: 92  FFALPPEEKLRFDMSGGKKGGFIVSSHLQGEAVQDWREIVTYFSYPVRNRDYSRWPDKPQ 151

Query: 126 GWIEKTQKFSEQMMELASKLLQVLSEAMDLDKEALKNACGELNQKILVNFYPKCLHSDLD 185
           GW + T+++SE++M LA KLL+VLSEAM L+KE+L NAC +++QKI+VN+YPKC   DL 
Sbjct: 152 GWAKVTEEYSEKLMGLACKLLEVLSEAMGLEKESLTNACVDMDQKIVVNYYPKCPQPDLT 211

Query: 186 RGLRRHTDPGTITILLQDQVSGLQATKDGGKTWMNIKPMEGAFVVNIGDHGHYLSNGRFK 245
            GL+RHTDPGTIT+LLQDQV GLQAT+D G TW+ ++P+EGAFVVN+GDHGH+LSNGRFK
Sbjct: 212 LGLKRHTDPGTITLLLQDQVGGLQATRDDGNTWITVQPVEGAFVVNLGDHGHFLSNGRFK 271

Query: 246 NADHRALASPNSNRISIAAFQYPAPEAIVYPLKVGEGEKPLLDTPISFQEMYNKKMSNEP 305
           NADH+A+ + NS+R+SIA FQ PAPEA VYPLKV EGEK +++ PI+F EMY +KM  + 
Sbjct: 272 NADHQAVVNSNSSRLSIATFQNPAPEATVYPLKVREGEKAIMEEPITFAEMYKRKMGRDL 331

Query: 306 TLAVLNN--KDPHNLKLSA 322
            LA L    K+ HN K +A
Sbjct: 332 ELARLKKLAKEEHNHKEAA 349

BLAST of Lcy01g008530 vs. ExPASy Swiss-Prot
Match: A0A4D6Q4T7 (Flavanone 3-dioxygenase F3H2 OS=Crocosmia x crocosmiiflora OX=1053288 GN=F3H-2 PE=1 SV=1)

HSP 1 Score: 415.2 bits (1066), Expect = 6.6e-115
Identity = 199/302 (65.89%), Postives = 242/302 (80.13%), Query Frame = 0

Query: 9   NEIPVVSLARIDDAADSHRAAVKKKIVEACEVWGIFQVVDHGVDPKLISDMIRLANEFFA 68
           +EIPV+SL  ID+  D  RA + KKIV ACE WG+FQVVDHGVD  LISDM RLA EFFA
Sbjct: 41  SEIPVISLEGIDE-VDGRRAEICKKIVAACEDWGVFQVVDHGVDAGLISDMTRLAREFFA 100

Query: 69  FPREQKLQFDMSAGKKGGFMIPGQRKGEA-QDWREIVMFFTYPIRNRNYSEWPDMPVGWI 128
            P E KL+FDM+ GKKGGF++    +GEA QDWREIV +F+YPIR R+YS WPD P  W 
Sbjct: 101 LPPEDKLRFDMTGGKKGGFIVSSHLQGEAVQDWREIVTYFSYPIRARDYSRWPDKPEDWR 160

Query: 129 EKTQKFSEQMMELASKLLQVLSEAMDLDKEALKNACGELNQKILVNFYPKCLHSDLDRGL 188
             T+ +SE++MELA KLL +LSEAM LD EAL  AC +++QK++VNFYPKC   DL  GL
Sbjct: 161 SVTKTYSEKLMELACKLLGILSEAMGLDTEALTKACVDMDQKVVVNFYPKCPQPDLTLGL 220

Query: 189 RRHTDPGTITILLQDQVSGLQATKDGGKTWMNIKPMEGAFVVNIGDHGHYLSNGRFKNAD 248
           +RHTDPGTIT+LLQDQV GLQATKDGGKTW+ ++P+EGAFVVN+GDHGH+LSNGRFKNAD
Sbjct: 221 KRHTDPGTITLLLQDQVGGLQATKDGGKTWITVQPVEGAFVVNLGDHGHFLSNGRFKNAD 280

Query: 249 HRALASPNSNRISIAAFQYPAPEAIVYPLKVGEGEKPLLDTPISFQEMYNKKMSNEPTLA 308
           H+A+ + N++R+SIA FQ PAP+AIVYPL + EGEKP+LD PI+F EMY +KMS +  LA
Sbjct: 281 HQAVVNSNTSRLSIATFQNPAPDAIVYPLAIREGEKPVLDEPITFAEMYRRKMSRDLELA 340

Query: 309 VL 310
            L
Sbjct: 341 NL 341

BLAST of Lcy01g008530 vs. ExPASy Swiss-Prot
Match: Q07353 (Naringenin,2-oxoglutarate 3-dioxygenase (Fragment) OS=Petunia hybrida OX=4102 GN=AN3 PE=1 SV=1)

HSP 1 Score: 415.2 bits (1066), Expect = 6.6e-115
Identity = 198/305 (64.92%), Postives = 242/305 (79.34%), Query Frame = 0

Query: 9   NEIPVVSLARIDDAADSHRAAVKKKIVEACEVWGIFQVVDHGVDPKLISDMIRLANEFFA 68
           NEIP++SL  IDD     RA +  KIV+ACE WG+FQVVDHGVD ++IS M   A EFFA
Sbjct: 39  NEIPIISLEGIDDET-GKRAEICDKIVKACEDWGVFQVVDHGVDAEVISQMTTFAKEFFA 98

Query: 69  FPREQKLQFDMSAGKKGGFMIPGQRKGE-AQDWREIVMFFTYPIRNRNYSEWPDMPVGWI 128
            P E+KL+FDMS GKKGGF++    +GE  QDWREIV +F+YP R R+YS WPD P GWI
Sbjct: 99  LPPEEKLRFDMSGGKKGGFIVSSHLQGEVVQDWREIVTYFSYPTRARDYSRWPDKPEGWI 158

Query: 129 EKTQKFSEQMMELASKLLQVLSEAMDLDKEALKNACGELNQKILVNFYPKCLHSDLDRGL 188
             TQK+SE++MELA KLL VLSEAM L+KEAL  AC +++QK++VNFYPKC   DL  GL
Sbjct: 159 AVTQKYSEKLMELACKLLDVLSEAMGLEKEALTKACVDMDQKVVVNFYPKCPEPDLTLGL 218

Query: 189 RRHTDPGTITILLQDQVSGLQATKDGGKTWMNIKPMEGAFVVNIGDHGHYLSNGRFKNAD 248
           +RHTDPGTIT+LLQDQV GLQATKD GKTW+ ++P+EGAFVVN+GDHGH+LSNGRFKNAD
Sbjct: 219 KRHTDPGTITLLLQDQVGGLQATKDNGKTWITVQPVEGAFVVNLGDHGHFLSNGRFKNAD 278

Query: 249 HRALASPNSNRISIAAFQYPAPEAIVYPLKVGEGEKPLLDTPISFQEMYNKKMSNEPTLA 308
           H+A+ + NS+R+SIA FQ PAPEAIVYPLK+ EGEK ++D PI+F EMY +KMS +  LA
Sbjct: 279 HQAVVNSNSSRLSIATFQNPAPEAIVYPLKIREGEKSIMDEPITFAEMYRRKMSKDLELA 338

Query: 309 VLNNK 313
            L  +
Sbjct: 339 RLKKQ 342

BLAST of Lcy01g008530 vs. ExPASy Swiss-Prot
Match: Q06942 (Naringenin,2-oxoglutarate 3-dioxygenase OS=Malus domestica OX=3750 PE=2 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 9.5e-114
Identity = 197/302 (65.23%), Postives = 242/302 (80.13%), Query Frame = 0

Query: 9   NEIPVVSLARIDDAADSHRAAVKKKIVEACEVWGIFQVVDHGVDPKLISDMIRLANEFFA 68
           NEIP++SLA ID+  +  R  + KKIV ACE WGIFQ+VDHGVD +LIS+M  LA EFFA
Sbjct: 37  NEIPIISLAGIDE-VEGRRGEICKKIVAACEDWGIFQIVDHGVDAELISEMTGLAREFFA 96

Query: 69  FPREQKLQFDMSAGKKGGFMIPGQRKGEA-QDWREIVMFFTYPIRNRNYSEWPDMPVGWI 128
            P E+KL+FDMS GKKGGF++    +GEA QDWREIV +F+YPIR+R+YS WPD P  W 
Sbjct: 97  LPSEEKLRFDMSGGKKGGFIVSSHLQGEAVQDWREIVTYFSYPIRHRDYSRWPDKPEAWR 156

Query: 129 EKTQKFSEQMMELASKLLQVLSEAMDLDKEALKNACGELNQKILVNFYPKCLHSDLDRGL 188
           E T+K+S+++M LA KLL VLSEAM LD EAL  AC +++QK++VNFYPKC   DL  GL
Sbjct: 157 EVTKKYSDELMGLACKLLGVLSEAMGLDTEALTKACVDMDQKVVVNFYPKCPQPDLTLGL 216

Query: 189 RRHTDPGTITILLQDQVSGLQATKDGGKTWMNIKPMEGAFVVNIGDHGHYLSNGRFKNAD 248
           +RHTDPGTIT+LLQDQV GLQAT+D GKTW+ ++P+EGAFVVN+GDHGH LSNGRFKNAD
Sbjct: 217 KRHTDPGTITLLLQDQVGGLQATRDDGKTWITVQPVEGAFVVNLGDHGHLLSNGRFKNAD 276

Query: 249 HRALASPNSNRISIAAFQYPAPEAIVYPLKVGEGEKPLLDTPISFQEMYNKKMSNEPTLA 308
           H+A+ + NS+R+SIA FQ PA EAIVYPL V EGEKP+L+ PI++ EMY KKMS +  LA
Sbjct: 277 HQAVVNSNSSRLSIATFQNPAQEAIVYPLSVREGEKPILEAPITYTEMYKKKMSKDLELA 336

Query: 309 VL 310
            L
Sbjct: 337 RL 337

BLAST of Lcy01g008530 vs. ExPASy TrEMBL
Match: A0A0A0KAQ6 (Flavanone-3-hydroxylase OS=Cucumis sativus OX=3659 GN=Csa_6G108510 PE=3 SV=1)

HSP 1 Score: 490.7 bits (1262), Expect = 4.6e-135
Identity = 233/320 (72.81%), Postives = 276/320 (86.25%), Query Frame = 0

Query: 1   MATFIEEENEIPVVSLARIDDAADSHRAAVKKKIVEACEVWGIFQVVDHGVDPKLISDMI 60
           MATF ++  EIPV+SL  I+D  D+H AAVKKKIVEACEVWGIFQVVDHGVDPKLI+DM+
Sbjct: 1   MATFTDD--EIPVISLDGIED--DAHMAAVKKKIVEACEVWGIFQVVDHGVDPKLIADMV 60

Query: 61  RLANEFFAFPREQKLQFDMSAGKKGGFMIPGQRKGEAQDWREIVMFFTYPIRNRNYSEWP 120
           RL  EF+ FP E+KL+FDMS GK+GGFMI  +R+G+ +DWRE+V FFTYP R+R YSEWP
Sbjct: 61  RLTTEFYYFPPEEKLRFDMSGGKRGGFMISTRRQGDVEDWREVVTFFTYPSRDRKYSEWP 120

Query: 121 DMPVGWIEKTQKFSEQMMELASKLLQVLSEAMDLDKEALKNACGELNQKILVNFYPKCLH 180
           D P GWI+KT++FSE++M+LASK+L+VLSEAMD++K ALK ACGELNQKIL NFYPKC +
Sbjct: 121 DKPEGWIKKTEEFSEKLMDLASKILEVLSEAMDMEKGALKKACGELNQKILTNFYPKCSN 180

Query: 181 SDLDRGLRRHTDPGTITILLQDQVSGLQATKDGGKTWMNIKPMEGAFVVNIGDHGHYLSN 240
           +DL+ GL RHTDPGTITILLQDQV GLQATKDG KTW+NI PMEGAFV+NIGDHGHYLSN
Sbjct: 181 TDLELGLPRHTDPGTITILLQDQVGGLQATKDGAKTWINIPPMEGAFVINIGDHGHYLSN 240

Query: 241 GRFKNADHRALASPNSNRISIAAFQYPAPEAIVYPLKVGEGEKPLLDTPISFQEMYNKKM 300
           GRF++ADHRALA+P S+R+SIA FQYP PEAIVYPLKVGEGEK +++ PISF EMY KKM
Sbjct: 241 GRFRSADHRALANPKSDRLSIATFQYPTPEAIVYPLKVGEGEKAVIEDPISFAEMYKKKM 300

Query: 301 SNEPTLAVLNNKDPHNLKLS 321
           SNEP LA+ N+K  + L  S
Sbjct: 301 SNEPVLAMFNHKGQNKLNPS 316

BLAST of Lcy01g008530 vs. ExPASy TrEMBL
Match: A0A5D3DMC6 (Naringenin,2-oxoglutarate 3-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G003110 PE=3 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 3.0e-134
Identity = 236/316 (74.68%), Postives = 271/316 (85.76%), Query Frame = 0

Query: 1   MATFIEEENEIPVVSLARIDDAADSHRAAVKKKIVEACEVWGIFQVVDHGVDPKLISDMI 60
           MATF E+  EIPV+SLA IDD  D+H AAVKKKIVEACEVWGIFQVVDHGVDPKLI DMI
Sbjct: 1   MATFTED--EIPVISLAGIDD--DAHMAAVKKKIVEACEVWGIFQVVDHGVDPKLIDDMI 60

Query: 61  RLANEFFAFPREQKLQFDMSAGKKGGFMIPGQRKGEAQDWREIVMFFTYPIRNRNYSEWP 120
           RL  EF+  P E+KL+FDMS GKKGGFMI  +RKG+ +DWRE+V FFTYP R+R YSEWP
Sbjct: 61  RLTTEFYYLPPEEKLRFDMSGGKKGGFMISTRRKGDVEDWREVVTFFTYPSRDRRYSEWP 120

Query: 121 DMPVGWIEKTQKFSEQMMELASKLLQVLSEAMDLDKEALKNACGELNQKILVNFYPKCLH 180
           D P GWI+ T++FSE++M+LASK+L+VLSEAMDL+K ALK ACGELNQKIL NFYPKC +
Sbjct: 121 DKPEGWIKTTEEFSERLMDLASKILEVLSEAMDLEKGALKKACGELNQKILTNFYPKCSN 180

Query: 181 SDLDRGLRRHTDPGTITILLQDQVSGLQATKDGGKTWMNIKPMEGAFVVNIGDHGHYLSN 240
           SDL+ GL RHTDPGTITILLQDQV GLQATKDG  +W+NI PM+GAFV+NIGDHGHYLSN
Sbjct: 181 SDLELGLPRHTDPGTITILLQDQVGGLQATKDGANSWINIPPMKGAFVINIGDHGHYLSN 240

Query: 241 GRFKNADHRALASPNSNRISIAAFQYPAPEAIVYPLKVGEGEKPLLDTPISFQEMYNKKM 300
           GRFK+ADHRALA+  S+R+SIA FQYP PEAIVYPLKVGEGEK +++ PISF EMY KKM
Sbjct: 241 GRFKSADHRALANAKSDRLSIATFQYPTPEAIVYPLKVGEGEKAVIENPISFAEMYKKKM 300

Query: 301 SNEPTLAVLNNKDPHN 317
           SNEP LA+ N+K  HN
Sbjct: 301 SNEPVLAMFNHKG-HN 311

BLAST of Lcy01g008530 vs. ExPASy TrEMBL
Match: A0A1S3CC29 (naringenin,2-oxoglutarate 3-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103498778 PE=3 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 3.0e-134
Identity = 236/316 (74.68%), Postives = 271/316 (85.76%), Query Frame = 0

Query: 1   MATFIEEENEIPVVSLARIDDAADSHRAAVKKKIVEACEVWGIFQVVDHGVDPKLISDMI 60
           MATF E+  EIPV+SLA IDD  D+H AAVKKKIVEACEVWGIFQVVDHGVDPKLI DMI
Sbjct: 1   MATFTED--EIPVISLAGIDD--DAHMAAVKKKIVEACEVWGIFQVVDHGVDPKLIDDMI 60

Query: 61  RLANEFFAFPREQKLQFDMSAGKKGGFMIPGQRKGEAQDWREIVMFFTYPIRNRNYSEWP 120
           RL  EF+  P E+KL+FDMS GKKGGFMI  +RKG+ +DWRE+V FFTYP R+R YSEWP
Sbjct: 61  RLTTEFYYLPPEEKLRFDMSGGKKGGFMISTRRKGDVEDWREVVTFFTYPSRDRRYSEWP 120

Query: 121 DMPVGWIEKTQKFSEQMMELASKLLQVLSEAMDLDKEALKNACGELNQKILVNFYPKCLH 180
           D P GWI+ T++FSE++M+LASK+L+VLSEAMDL+K ALK ACGELNQKIL NFYPKC +
Sbjct: 121 DKPEGWIKTTEEFSERLMDLASKILEVLSEAMDLEKGALKKACGELNQKILTNFYPKCSN 180

Query: 181 SDLDRGLRRHTDPGTITILLQDQVSGLQATKDGGKTWMNIKPMEGAFVVNIGDHGHYLSN 240
           SDL+ GL RHTDPGTITILLQDQV GLQATKDG  +W+NI PM+GAFV+NIGDHGHYLSN
Sbjct: 181 SDLELGLPRHTDPGTITILLQDQVGGLQATKDGANSWINIPPMKGAFVINIGDHGHYLSN 240

Query: 241 GRFKNADHRALASPNSNRISIAAFQYPAPEAIVYPLKVGEGEKPLLDTPISFQEMYNKKM 300
           GRFK+ADHRALA+  S+R+SIA FQYP PEAIVYPLKVGEGEK +++ PISF EMY KKM
Sbjct: 241 GRFKSADHRALANAKSDRLSIATFQYPTPEAIVYPLKVGEGEKAVIENPISFAEMYKKKM 300

Query: 301 SNEPTLAVLNNKDPHN 317
           SNEP LA+ N+K  HN
Sbjct: 301 SNEPVLAMFNHKG-HN 311

BLAST of Lcy01g008530 vs. ExPASy TrEMBL
Match: A0A5A7TET3 (Naringenin,2-oxoglutarate 3-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold122G00090 PE=3 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 1.4e-131
Identity = 236/328 (71.95%), Postives = 271/328 (82.62%), Query Frame = 0

Query: 1   MATFIEEENEIPVVSLARIDDAADSHRAAVKKKIVEACEVWGIFQVVDHGVDPKLISDMI 60
           MATF E+  EIPV+SLA IDD  D+H AAVKKKIVEACEVWGIFQVVDHGVDPKLI DMI
Sbjct: 1   MATFTED--EIPVISLAGIDD--DAHMAAVKKKIVEACEVWGIFQVVDHGVDPKLIDDMI 60

Query: 61  RLANEFFAFPREQKLQFDMSAGKKGGFMIPGQRKGEAQDWREIVMFFTYPIRNRNYSEWP 120
           RL  EF+  P E+KL+FDMS GKKGGFMI  +RKG+ +DWRE+V FFTYP R+R YSEWP
Sbjct: 61  RLTTEFYYLPPEEKLRFDMSGGKKGGFMISTRRKGDVEDWREVVTFFTYPSRDRRYSEWP 120

Query: 121 DMPVGWIEKTQKFSEQMMELASKLLQVLSEAMDLDKEALKNACGELNQKILVNFYPKCLH 180
           D P GWI+ T++FSE++M+LASK+L+VLSEAMDL+K ALK ACGELNQKIL NFYPKC +
Sbjct: 121 DKPEGWIKTTEEFSERLMDLASKILEVLSEAMDLEKGALKKACGELNQKILTNFYPKCSN 180

Query: 181 SDLDRGLRRHTDPGTITILLQ------------DQVSGLQATKDGGKTWMNIKPMEGAFV 240
           SDL+ GL RHTDPGTITILLQ            DQV GLQATKDG  +W+NI PM+GAFV
Sbjct: 181 SDLELGLPRHTDPGTITILLQATSHSLYIYLISDQVGGLQATKDGANSWINIPPMKGAFV 240

Query: 241 VNIGDHGHYLSNGRFKNADHRALASPNSNRISIAAFQYPAPEAIVYPLKVGEGEKPLLDT 300
           +NIGDHGHYLSNGRFK+ADHRALA+  S+R+SIA FQYP PEAIVYPLKVGEGEK +++ 
Sbjct: 241 INIGDHGHYLSNGRFKSADHRALANAKSDRLSIATFQYPTPEAIVYPLKVGEGEKAVIEN 300

Query: 301 PISFQEMYNKKMSNEPTLAVLNNKDPHN 317
           PISF EMY KKMSNEP LA+ N+K  HN
Sbjct: 301 PISFAEMYKKKMSNEPVLAMFNHKG-HN 323

BLAST of Lcy01g008530 vs. ExPASy TrEMBL
Match: A0A6J1CU28 (naringenin,2-oxoglutarate 3-dioxygenase OS=Momordica charantia OX=3673 GN=LOC111014783 PE=3 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 2.3e-118
Identity = 205/302 (67.88%), Postives = 250/302 (82.78%), Query Frame = 0

Query: 9   NEIPVVSLARIDDAADSHRAAVKKKIVEACEVWGIFQVVDHGVDPKLISDMIRLANEFFA 68
           +E+P++SLA IDDA    RA + +KIVEACE WGIFQVVDHGVD  LIS+M RLA EFFA
Sbjct: 112 DEVPIISLAGIDDAEGGRRAEICRKIVEACEDWGIFQVVDHGVDAALISEMSRLATEFFA 171

Query: 69  FPREQKLQFDMSAGKKGGFMIPGQRKGEA-QDWREIVMFFTYPIRNRNYSEWPDMPVGWI 128
            P E+KL+FDMS GKKGGF++    KGEA QDWREIV +F+YPIRNR+YS WPD P GW+
Sbjct: 172 LPPEEKLRFDMSGGKKGGFIVSSHLKGEAVQDWREIVTYFSYPIRNRDYSRWPDKPEGWV 231

Query: 129 EKTQKFSEQMMELASKLLQVLSEAMDLDKEALKNACGELNQKILVNFYPKCLHSDLDRGL 188
            KT+++SE++M LA KLL+VLSEAM L+KEAL NAC +++QKI+VNFYPKC   DL  GL
Sbjct: 232 AKTEEYSEKLMGLACKLLEVLSEAMGLEKEALTNACVDMDQKIVVNFYPKCPQPDLTLGL 291

Query: 189 RRHTDPGTITILLQDQVSGLQATKDGGKTWMNIKPMEGAFVVNIGDHGHYLSNGRFKNAD 248
           +RHTDPGTIT+LLQDQV GLQATKD G+TW+ ++P+ GAFVVN+GDHGHYLSNGRFKNAD
Sbjct: 292 KRHTDPGTITLLLQDQVGGLQATKDNGRTWITVQPVPGAFVVNLGDHGHYLSNGRFKNAD 351

Query: 249 HRALASPNSNRISIAAFQYPAPEAIVYPLKVGEGEKPLLDTPISFQEMYNKKMSNEPTLA 308
           H+A+ +  S+R+SIA FQ PAPEAIVYPLKV EGE P+L++PI+F EMY +KMS +  LA
Sbjct: 352 HQAVVNSKSSRLSIATFQNPAPEAIVYPLKVREGENPILESPITFSEMYQRKMSRDIELA 411

Query: 309 VL 310
            L
Sbjct: 412 RL 413

BLAST of Lcy01g008530 vs. NCBI nr
Match: XP_004140496.1 (naringenin,2-oxoglutarate 3-dioxygenase [Cucumis sativus] >KGN46553.1 hypothetical protein Csa_005088 [Cucumis sativus])

HSP 1 Score: 490.7 bits (1262), Expect = 9.4e-135
Identity = 233/320 (72.81%), Postives = 276/320 (86.25%), Query Frame = 0

Query: 1   MATFIEEENEIPVVSLARIDDAADSHRAAVKKKIVEACEVWGIFQVVDHGVDPKLISDMI 60
           MATF ++  EIPV+SL  I+D  D+H AAVKKKIVEACEVWGIFQVVDHGVDPKLI+DM+
Sbjct: 1   MATFTDD--EIPVISLDGIED--DAHMAAVKKKIVEACEVWGIFQVVDHGVDPKLIADMV 60

Query: 61  RLANEFFAFPREQKLQFDMSAGKKGGFMIPGQRKGEAQDWREIVMFFTYPIRNRNYSEWP 120
           RL  EF+ FP E+KL+FDMS GK+GGFMI  +R+G+ +DWRE+V FFTYP R+R YSEWP
Sbjct: 61  RLTTEFYYFPPEEKLRFDMSGGKRGGFMISTRRQGDVEDWREVVTFFTYPSRDRKYSEWP 120

Query: 121 DMPVGWIEKTQKFSEQMMELASKLLQVLSEAMDLDKEALKNACGELNQKILVNFYPKCLH 180
           D P GWI+KT++FSE++M+LASK+L+VLSEAMD++K ALK ACGELNQKIL NFYPKC +
Sbjct: 121 DKPEGWIKKTEEFSEKLMDLASKILEVLSEAMDMEKGALKKACGELNQKILTNFYPKCSN 180

Query: 181 SDLDRGLRRHTDPGTITILLQDQVSGLQATKDGGKTWMNIKPMEGAFVVNIGDHGHYLSN 240
           +DL+ GL RHTDPGTITILLQDQV GLQATKDG KTW+NI PMEGAFV+NIGDHGHYLSN
Sbjct: 181 TDLELGLPRHTDPGTITILLQDQVGGLQATKDGAKTWINIPPMEGAFVINIGDHGHYLSN 240

Query: 241 GRFKNADHRALASPNSNRISIAAFQYPAPEAIVYPLKVGEGEKPLLDTPISFQEMYNKKM 300
           GRF++ADHRALA+P S+R+SIA FQYP PEAIVYPLKVGEGEK +++ PISF EMY KKM
Sbjct: 241 GRFRSADHRALANPKSDRLSIATFQYPTPEAIVYPLKVGEGEKAVIEDPISFAEMYKKKM 300

Query: 301 SNEPTLAVLNNKDPHNLKLS 321
           SNEP LA+ N+K  + L  S
Sbjct: 301 SNEPVLAMFNHKGQNKLNPS 316

BLAST of Lcy01g008530 vs. NCBI nr
Match: XP_008459735.1 (PREDICTED: naringenin,2-oxoglutarate 3-dioxygenase-like [Cucumis melo] >TYK24748.1 naringenin,2-oxoglutarate 3-dioxygenase-like [Cucumis melo var. makuwa])

HSP 1 Score: 488.0 bits (1255), Expect = 6.1e-134
Identity = 236/316 (74.68%), Postives = 271/316 (85.76%), Query Frame = 0

Query: 1   MATFIEEENEIPVVSLARIDDAADSHRAAVKKKIVEACEVWGIFQVVDHGVDPKLISDMI 60
           MATF E+  EIPV+SLA IDD  D+H AAVKKKIVEACEVWGIFQVVDHGVDPKLI DMI
Sbjct: 1   MATFTED--EIPVISLAGIDD--DAHMAAVKKKIVEACEVWGIFQVVDHGVDPKLIDDMI 60

Query: 61  RLANEFFAFPREQKLQFDMSAGKKGGFMIPGQRKGEAQDWREIVMFFTYPIRNRNYSEWP 120
           RL  EF+  P E+KL+FDMS GKKGGFMI  +RKG+ +DWRE+V FFTYP R+R YSEWP
Sbjct: 61  RLTTEFYYLPPEEKLRFDMSGGKKGGFMISTRRKGDVEDWREVVTFFTYPSRDRRYSEWP 120

Query: 121 DMPVGWIEKTQKFSEQMMELASKLLQVLSEAMDLDKEALKNACGELNQKILVNFYPKCLH 180
           D P GWI+ T++FSE++M+LASK+L+VLSEAMDL+K ALK ACGELNQKIL NFYPKC +
Sbjct: 121 DKPEGWIKTTEEFSERLMDLASKILEVLSEAMDLEKGALKKACGELNQKILTNFYPKCSN 180

Query: 181 SDLDRGLRRHTDPGTITILLQDQVSGLQATKDGGKTWMNIKPMEGAFVVNIGDHGHYLSN 240
           SDL+ GL RHTDPGTITILLQDQV GLQATKDG  +W+NI PM+GAFV+NIGDHGHYLSN
Sbjct: 181 SDLELGLPRHTDPGTITILLQDQVGGLQATKDGANSWINIPPMKGAFVINIGDHGHYLSN 240

Query: 241 GRFKNADHRALASPNSNRISIAAFQYPAPEAIVYPLKVGEGEKPLLDTPISFQEMYNKKM 300
           GRFK+ADHRALA+  S+R+SIA FQYP PEAIVYPLKVGEGEK +++ PISF EMY KKM
Sbjct: 241 GRFKSADHRALANAKSDRLSIATFQYPTPEAIVYPLKVGEGEKAVIENPISFAEMYKKKM 300

Query: 301 SNEPTLAVLNNKDPHN 317
           SNEP LA+ N+K  HN
Sbjct: 301 SNEPVLAMFNHKG-HN 311

BLAST of Lcy01g008530 vs. NCBI nr
Match: XP_038875953.1 (naringenin,2-oxoglutarate 3-dioxygenase-like [Benincasa hispida])

HSP 1 Score: 482.6 bits (1241), Expect = 2.6e-132
Identity = 231/312 (74.04%), Postives = 269/312 (86.22%), Query Frame = 0

Query: 1   MATFIEEENEIPVVSLARIDDAADSHRAAVKKKIVEACEVWGIFQVVDHGVDPKLISDMI 60
           MA+F  ++++IPV+SLA IDD  D    AVKKKIVEACE WGIF +VDHG+DPKLI+DMI
Sbjct: 1   MASF-TDDHQIPVISLAGIDD--DGRMPAVKKKIVEACEAWGIFLMVDHGIDPKLIADMI 60

Query: 61  RLANEFFAFPREQKLQFDMSAGKKGGFMIPGQRKGEAQDWREIVMFFTYPIRNRNYSEWP 120
           RL  EF+  PRE+KL+FDMS+GK+GGFMI  +RKGE +DWRE+V FFTYP R+RNYSEWP
Sbjct: 61  RLTTEFYYLPREEKLRFDMSSGKRGGFMISTRRKGEVEDWREVVTFFTYPSRDRNYSEWP 120

Query: 121 DMPVGWIEKTQKFSEQMMELASKLLQVLSEAMDLDKEALKNACGELNQKILVNFYPKCLH 180
           D P  WI  T++FSE++M+LASK+L+VLSEAMDL+K ALK ACGELNQKIL NFYPKC +
Sbjct: 121 DKPEDWIGTTKEFSEKLMDLASKILEVLSEAMDLEKGALKKACGELNQKILTNFYPKCSN 180

Query: 181 SDLDRGLRRHTDPGTITILLQDQVSGLQATKDGGKTWMNIKPMEGAFVVNIGDHGHYLSN 240
           SDL+ GL RHTDPGTITILLQDQV GLQATKDG K+W+NI PMEGAFVVNIGDHGHYLSN
Sbjct: 181 SDLELGLPRHTDPGTITILLQDQVGGLQATKDGAKSWINIPPMEGAFVVNIGDHGHYLSN 240

Query: 241 GRFKNADHRALASPNSNRISIAAFQYPAPEAIVYPLKVGEGEKPLLDTPISFQEMYNKKM 300
           GRFK+ADHRALA+  SNR+SIA FQYPAPEAIVYPLKV EGEKP+++ PISFQEMY KKM
Sbjct: 241 GRFKSADHRALANSKSNRLSIATFQYPAPEAIVYPLKVEEGEKPVIENPISFQEMYKKKM 300

Query: 301 SNEPTLAVLNNK 313
           S EP LA+ N+K
Sbjct: 301 SKEPVLAMFNHK 309

BLAST of Lcy01g008530 vs. NCBI nr
Match: KAA0039749.1 (naringenin,2-oxoglutarate 3-dioxygenase-like [Cucumis melo var. makuwa])

HSP 1 Score: 479.2 bits (1232), Expect = 2.8e-131
Identity = 236/328 (71.95%), Postives = 271/328 (82.62%), Query Frame = 0

Query: 1   MATFIEEENEIPVVSLARIDDAADSHRAAVKKKIVEACEVWGIFQVVDHGVDPKLISDMI 60
           MATF E+  EIPV+SLA IDD  D+H AAVKKKIVEACEVWGIFQVVDHGVDPKLI DMI
Sbjct: 1   MATFTED--EIPVISLAGIDD--DAHMAAVKKKIVEACEVWGIFQVVDHGVDPKLIDDMI 60

Query: 61  RLANEFFAFPREQKLQFDMSAGKKGGFMIPGQRKGEAQDWREIVMFFTYPIRNRNYSEWP 120
           RL  EF+  P E+KL+FDMS GKKGGFMI  +RKG+ +DWRE+V FFTYP R+R YSEWP
Sbjct: 61  RLTTEFYYLPPEEKLRFDMSGGKKGGFMISTRRKGDVEDWREVVTFFTYPSRDRRYSEWP 120

Query: 121 DMPVGWIEKTQKFSEQMMELASKLLQVLSEAMDLDKEALKNACGELNQKILVNFYPKCLH 180
           D P GWI+ T++FSE++M+LASK+L+VLSEAMDL+K ALK ACGELNQKIL NFYPKC +
Sbjct: 121 DKPEGWIKTTEEFSERLMDLASKILEVLSEAMDLEKGALKKACGELNQKILTNFYPKCSN 180

Query: 181 SDLDRGLRRHTDPGTITILLQ------------DQVSGLQATKDGGKTWMNIKPMEGAFV 240
           SDL+ GL RHTDPGTITILLQ            DQV GLQATKDG  +W+NI PM+GAFV
Sbjct: 181 SDLELGLPRHTDPGTITILLQATSHSLYIYLISDQVGGLQATKDGANSWINIPPMKGAFV 240

Query: 241 VNIGDHGHYLSNGRFKNADHRALASPNSNRISIAAFQYPAPEAIVYPLKVGEGEKPLLDT 300
           +NIGDHGHYLSNGRFK+ADHRALA+  S+R+SIA FQYP PEAIVYPLKVGEGEK +++ 
Sbjct: 241 INIGDHGHYLSNGRFKSADHRALANAKSDRLSIATFQYPTPEAIVYPLKVGEGEKAVIEN 300

Query: 301 PISFQEMYNKKMSNEPTLAVLNNKDPHN 317
           PISF EMY KKMSNEP LA+ N+K  HN
Sbjct: 301 PISFAEMYKKKMSNEPVLAMFNHKG-HN 323

BLAST of Lcy01g008530 vs. NCBI nr
Match: XP_022145295.1 (naringenin,2-oxoglutarate 3-dioxygenase [Momordica charantia])

HSP 1 Score: 435.3 bits (1118), Expect = 4.7e-118
Identity = 205/302 (67.88%), Postives = 250/302 (82.78%), Query Frame = 0

Query: 9   NEIPVVSLARIDDAADSHRAAVKKKIVEACEVWGIFQVVDHGVDPKLISDMIRLANEFFA 68
           +E+P++SLA IDDA    RA + +KIVEACE WGIFQVVDHGVD  LIS+M RLA EFFA
Sbjct: 112 DEVPIISLAGIDDAEGGRRAEICRKIVEACEDWGIFQVVDHGVDAALISEMSRLATEFFA 171

Query: 69  FPREQKLQFDMSAGKKGGFMIPGQRKGEA-QDWREIVMFFTYPIRNRNYSEWPDMPVGWI 128
            P E+KL+FDMS GKKGGF++    KGEA QDWREIV +F+YPIRNR+YS WPD P GW+
Sbjct: 172 LPPEEKLRFDMSGGKKGGFIVSSHLKGEAVQDWREIVTYFSYPIRNRDYSRWPDKPEGWV 231

Query: 129 EKTQKFSEQMMELASKLLQVLSEAMDLDKEALKNACGELNQKILVNFYPKCLHSDLDRGL 188
            KT+++SE++M LA KLL+VLSEAM L+KEAL NAC +++QKI+VNFYPKC   DL  GL
Sbjct: 232 AKTEEYSEKLMGLACKLLEVLSEAMGLEKEALTNACVDMDQKIVVNFYPKCPQPDLTLGL 291

Query: 189 RRHTDPGTITILLQDQVSGLQATKDGGKTWMNIKPMEGAFVVNIGDHGHYLSNGRFKNAD 248
           +RHTDPGTIT+LLQDQV GLQATKD G+TW+ ++P+ GAFVVN+GDHGHYLSNGRFKNAD
Sbjct: 292 KRHTDPGTITLLLQDQVGGLQATKDNGRTWITVQPVPGAFVVNLGDHGHYLSNGRFKNAD 351

Query: 249 HRALASPNSNRISIAAFQYPAPEAIVYPLKVGEGEKPLLDTPISFQEMYNKKMSNEPTLA 308
           H+A+ +  S+R+SIA FQ PAPEAIVYPLKV EGE P+L++PI+F EMY +KMS +  LA
Sbjct: 352 HQAVVNSKSSRLSIATFQNPAPEAIVYPLKVREGENPILESPITFSEMYQRKMSRDIELA 411

Query: 309 VL 310
            L
Sbjct: 412 RL 413

BLAST of Lcy01g008530 vs. TAIR 10
Match: AT3G51240.1 (flavanone 3-hydroxylase )

HSP 1 Score: 419.1 bits (1076), Expect = 3.2e-117
Identity = 196/302 (64.90%), Postives = 247/302 (81.79%), Query Frame = 0

Query: 9   NEIPVVSLARIDDAADSHRAAVKKKIVEACEVWGIFQVVDHGVDPKLISDMIRLANEFFA 68
           +EIPV+SLA IDD  D  R  + ++IVEACE WGIFQVVDHGVD  L++DM RLA +FFA
Sbjct: 36  DEIPVISLAGIDD-VDGKRGEICRQIVEACENWGIFQVVDHGVDTNLVADMTRLARDFFA 95

Query: 69  FPREQKLQFDMSAGKKGGFMIPGQRKGEA-QDWREIVMFFTYPIRNRNYSEWPDMPVGWI 128
            P E KL+FDMS GKKGGF++    +GEA QDWREIV +F+YP+RNR+YS WPD P GW+
Sbjct: 96  LPPEDKLRFDMSGGKKGGFIVSSHLQGEAVQDWREIVTYFSYPVRNRDYSRWPDKPEGWV 155

Query: 129 EKTQKFSEQMMELASKLLQVLSEAMDLDKEALKNACGELNQKILVNFYPKCLHSDLDRGL 188
           + T+++SE++M LA KLL+VLSEAM L+KE+L NAC +++QKI+VN+YPKC   DL  GL
Sbjct: 156 KVTEEYSERLMSLACKLLEVLSEAMGLEKESLTNACVDMDQKIVVNYYPKCPQPDLTLGL 215

Query: 189 RRHTDPGTITILLQDQVSGLQATKDGGKTWMNIKPMEGAFVVNIGDHGHYLSNGRFKNAD 248
           +RHTDPGTIT+LLQDQV GLQAT+D GKTW+ ++P+EGAFVVN+GDHGH+LSNGRFKNAD
Sbjct: 216 KRHTDPGTITLLLQDQVGGLQATRDNGKTWITVQPVEGAFVVNLGDHGHFLSNGRFKNAD 275

Query: 249 HRALASPNSNRISIAAFQYPAPEAIVYPLKVGEGEKPLLDTPISFQEMYNKKMSNEPTLA 308
           H+A+ + NS+R+SIA FQ PAP+A VYPLKV EGEK +L+ PI+F EMY +KM  +  LA
Sbjct: 276 HQAVVNSNSSRLSIATFQNPAPDATVYPLKVREGEKAILEEPITFAEMYKRKMGRDLELA 335

Query: 309 VL 310
            L
Sbjct: 336 RL 336

BLAST of Lcy01g008530 vs. TAIR 10
Match: AT3G51240.2 (flavanone 3-hydroxylase )

HSP 1 Score: 356.3 bits (913), Expect = 2.6e-98
Identity = 164/252 (65.08%), Postives = 208/252 (82.54%), Query Frame = 0

Query: 59  MIRLANEFFAFPREQKLQFDMSAGKKGGFMIPGQRKGEA-QDWREIVMFFTYPIRNRNYS 118
           M RLA +FFA P E KL+FDMS GKKGGF++    +GEA QDWREIV +F+YP+RNR+YS
Sbjct: 1   MTRLARDFFALPPEDKLRFDMSGGKKGGFIVSSHLQGEAVQDWREIVTYFSYPVRNRDYS 60

Query: 119 EWPDMPVGWIEKTQKFSEQMMELASKLLQVLSEAMDLDKEALKNACGELNQKILVNFYPK 178
            WPD P GW++ T+++SE++M LA KLL+VLSEAM L+KE+L NAC +++QKI+VN+YPK
Sbjct: 61  RWPDKPEGWVKVTEEYSERLMSLACKLLEVLSEAMGLEKESLTNACVDMDQKIVVNYYPK 120

Query: 179 CLHSDLDRGLRRHTDPGTITILLQDQVSGLQATKDGGKTWMNIKPMEGAFVVNIGDHGHY 238
           C   DL  GL+RHTDPGTIT+LLQDQV GLQAT+D GKTW+ ++P+EGAFVVN+GDHGH+
Sbjct: 121 CPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRDNGKTWITVQPVEGAFVVNLGDHGHF 180

Query: 239 LSNGRFKNADHRALASPNSNRISIAAFQYPAPEAIVYPLKVGEGEKPLLDTPISFQEMYN 298
           LSNGRFKNADH+A+ + NS+R+SIA FQ PAP+A VYPLKV EGEK +L+ PI+F EMY 
Sbjct: 181 LSNGRFKNADHQAVVNSNSSRLSIATFQNPAPDATVYPLKVREGEKAILEEPITFAEMYK 240

Query: 299 KKMSNEPTLAVL 310
           +KM  +  LA L
Sbjct: 241 RKMGRDLELARL 252

BLAST of Lcy01g008530 vs. TAIR 10
Match: AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 178.7 bits (452), Expect = 7.4e-45
Identity = 97/276 (35.14%), Postives = 159/276 (57.61%), Query Frame = 0

Query: 5   IEEENEIPVVSLARIDDAADSHRAAVKKKIVEACEVWGIFQVVDHGVDPKLISDMIRLAN 64
           + +  + P++ L+  D      R+ + ++I +AC  +G FQV++HGV+ ++I +M+ +A 
Sbjct: 32  VSQLEDFPLIDLSSTD------RSFLIQQIHQACARFGFFQVINHGVNKQIIDEMVSVAR 91

Query: 65  EFFAFPREQKLQFDMSAGKKGGFMIP--GQRKGEAQDWREIVMFFTYPIRNRNYSEWPDM 124
           EFF+   E+K++       K   +      +K E  +WR+ +    YPI ++  +EWP  
Sbjct: 92  EFFSMSMEEKMKLYSDDPTKTTRLSTSFNVKKEEVNNWRDYLRLHCYPI-HKYVNEWPSN 151

Query: 125 PVGWIEKTQKFSEQMMELASKLLQVLSEAMDLDKEALKNACGELNQKILVNFYPKCLHSD 184
           P  + E   K+S ++ E+  K+ +++SE++ L+K+ +K   GE  Q + VN+YP C   +
Sbjct: 152 PPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQGQHMAVNYYPPCPEPE 211

Query: 185 LDRGLRRHTDPGTITILLQD-QVSGLQATKDGGKTWMNIKPMEGAFVVNIGDHGHYLSNG 244
           L  GL  HTDP  +TILLQD  V GLQ   DG   W  + P   AFV+NIGD    LSNG
Sbjct: 212 LTYGLPAHTDPNALTILLQDTTVCGLQILIDG--QWFAVNPHPDAFVINIGDQLQALSNG 271

Query: 245 RFKNADHRALASPNSNRISIAAFQYPAPEAIVYPLK 278
            +K+  HRA+ +  + R+S+A+F  PA  A++ P K
Sbjct: 272 VYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAK 298

BLAST of Lcy01g008530 vs. TAIR 10
Match: AT3G11180.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 176.0 bits (445), Expect = 4.8e-44
Identity = 99/298 (33.22%), Postives = 160/298 (53.69%), Query Frame = 0

Query: 3   TFIEEENEIPVVSLARID-DAADSHRAAVKKKIVEACEVWGIFQVVDHGVDPKLISDMIR 62
           T I+ + E+  +++  ID D+  S     KK+I EAC  WG FQV++HGV P+L+     
Sbjct: 82  TIIDHQPEVADINIPIIDLDSLFSGNEDDKKRISEACREWGFFQVINHGVKPELMDAARE 141

Query: 63  LANEFFAFPREQKLQFDMSAGKKGGF-MIPGQRKGEAQDWREIVMFFTYPIRNRNYSEWP 122
               FF  P E K  +  S     G+    G  KG   DW +       P+  +++++WP
Sbjct: 142 TWKSFFNLPVEAKEVYSNSPRTYEGYGSRLGVEKGAILDWNDYYYLHFLPLALKDFNKWP 201

Query: 123 DMPVGWIEKTQKFSEQMMELASKLLQVLSEAMDLDKEALKNACG--ELNQKILVNFYPKC 182
            +P    E   ++ +++++L  +L+ +LS  + L  E L+ A G  ++   + VN+YPKC
Sbjct: 202 SLPSNIREMNDEYGKELVKLGGRLMTILSSNLGLRAEQLQEAFGGEDVGACLRVNYYPKC 261

Query: 183 LHSDLDRGLRRHTDPGTITILL-QDQVSGLQATKDGGKTWMNIKPMEGAFVVNIGDHGHY 242
              +L  GL  H+DPG +TILL  DQV GLQ     G TW+ + P+  AF+VNIGD    
Sbjct: 262 PQPELALGLSPHSDPGGMTILLPDDQVVGLQVRH--GDTWITVNPLRHAFIVNIGDQIQI 321

Query: 243 LSNGRFKNADHRALASPNSNRISIAAFQYPAPEAIVYPL-KVGEGEKPLLDTPISFQE 295
           LSN ++K+ +HR + +    R+S+A F  P  +  + P+ ++     P L  P++F +
Sbjct: 322 LSNSKYKSVEHRVIVNSEKERVSLAFFYNPKSDIPIQPMQQLVTSTMPPLYPPMTFDQ 377

BLAST of Lcy01g008530 vs. TAIR 10
Match: AT3G11180.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 176.0 bits (445), Expect = 4.8e-44
Identity = 99/298 (33.22%), Postives = 160/298 (53.69%), Query Frame = 0

Query: 3   TFIEEENEIPVVSLARID-DAADSHRAAVKKKIVEACEVWGIFQVVDHGVDPKLISDMIR 62
           T I+ + E+  +++  ID D+  S     KK+I EAC  WG FQV++HGV P+L+     
Sbjct: 82  TIIDHQPEVADINIPIIDLDSLFSGNEDDKKRISEACREWGFFQVINHGVKPELMDAARE 141

Query: 63  LANEFFAFPREQKLQFDMSAGKKGGF-MIPGQRKGEAQDWREIVMFFTYPIRNRNYSEWP 122
               FF  P E K  +  S     G+    G  KG   DW +       P+  +++++WP
Sbjct: 142 TWKSFFNLPVEAKEVYSNSPRTYEGYGSRLGVEKGAILDWNDYYYLHFLPLALKDFNKWP 201

Query: 123 DMPVGWIEKTQKFSEQMMELASKLLQVLSEAMDLDKEALKNACG--ELNQKILVNFYPKC 182
            +P    E   ++ +++++L  +L+ +LS  + L  E L+ A G  ++   + VN+YPKC
Sbjct: 202 SLPSNIREMNDEYGKELVKLGGRLMTILSSNLGLRAEQLQEAFGGEDVGACLRVNYYPKC 261

Query: 183 LHSDLDRGLRRHTDPGTITILL-QDQVSGLQATKDGGKTWMNIKPMEGAFVVNIGDHGHY 242
              +L  GL  H+DPG +TILL  DQV GLQ     G TW+ + P+  AF+VNIGD    
Sbjct: 262 PQPELALGLSPHSDPGGMTILLPDDQVVGLQVRH--GDTWITVNPLRHAFIVNIGDQIQI 321

Query: 243 LSNGRFKNADHRALASPNSNRISIAAFQYPAPEAIVYPL-KVGEGEKPLLDTPISFQE 295
           LSN ++K+ +HR + +    R+S+A F  P  +  + P+ ++     P L  P++F +
Sbjct: 322 LSNSKYKSVEHRVIVNSEKERVSLAFFYNPKSDIPIQPMQQLVTSTMPPLYPPMTFDQ 377

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9S8184.6e-11664.90Naringenin,2-oxoglutarate 3-dioxygenase OS=Arabidopsis thaliana OX=3702 GN=F3H P... [more]
Q059654.6e-11663.01Naringenin,2-oxoglutarate 3-dioxygenase (Fragment) OS=Matthiola incana OX=3724 G... [more]
A0A4D6Q4T76.6e-11565.89Flavanone 3-dioxygenase F3H2 OS=Crocosmia x crocosmiiflora OX=1053288 GN=F3H-2 P... [more]
Q073536.6e-11564.92Naringenin,2-oxoglutarate 3-dioxygenase (Fragment) OS=Petunia hybrida OX=4102 GN... [more]
Q069429.5e-11465.23Naringenin,2-oxoglutarate 3-dioxygenase OS=Malus domestica OX=3750 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KAQ64.6e-13572.81Flavanone-3-hydroxylase OS=Cucumis sativus OX=3659 GN=Csa_6G108510 PE=3 SV=1[more]
A0A5D3DMC63.0e-13474.68Naringenin,2-oxoglutarate 3-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S3CC293.0e-13474.68naringenin,2-oxoglutarate 3-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A5A7TET31.4e-13171.95Naringenin,2-oxoglutarate 3-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194... [more]
A0A6J1CU282.3e-11867.88naringenin,2-oxoglutarate 3-dioxygenase OS=Momordica charantia OX=3673 GN=LOC111... [more]
Match NameE-valueIdentityDescription
XP_004140496.19.4e-13572.81naringenin,2-oxoglutarate 3-dioxygenase [Cucumis sativus] >KGN46553.1 hypothetic... [more]
XP_008459735.16.1e-13474.68PREDICTED: naringenin,2-oxoglutarate 3-dioxygenase-like [Cucumis melo] >TYK24748... [more]
XP_038875953.12.6e-13274.04naringenin,2-oxoglutarate 3-dioxygenase-like [Benincasa hispida][more]
KAA0039749.12.8e-13171.95naringenin,2-oxoglutarate 3-dioxygenase-like [Cucumis melo var. makuwa][more]
XP_022145295.14.7e-11867.88naringenin,2-oxoglutarate 3-dioxygenase [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT3G51240.13.2e-11764.90flavanone 3-hydroxylase [more]
AT3G51240.22.6e-9865.08flavanone 3-hydroxylase [more]
AT5G24530.17.4e-4535.142-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G11180.14.8e-4433.222-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G11180.24.8e-4433.222-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 170..264
e-value: 1.6E-19
score: 70.2
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 11..121
e-value: 1.7E-21
score: 77.1
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 2..305
e-value: 2.4E-78
score: 265.7
NoneNo IPR availablePANTHERPTHR47990:SF11NARINGENIN,2-OXOGLUTARATE 3-DIOXYGENASEcoord: 8..310
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 8..310
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 8..301
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 166..267
score: 11.976571

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy01g008530.1Lcy01g008530.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding
molecular_function GO:0045486 naringenin 3-dioxygenase activity