Lcy01g008320 (gene) Sponge gourd (P93075) v1

BLAST of Lcy01g008320 vs. ExPASy Swiss-Prot
Match: Q66S17 (Natterin-3 OS=Thalassophryne nattereri OX=289382 PE=1 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 3.5e-08
Identity = 43/154 (27.92%), Postives = 67/154 (43.51%), Query Frame = 0

Query: 282 LTVEEAILSRSIYNVQYRLSEGRIYGENIITMDFQGAMNDSNETNTMGLKFSYLQEESQS 341
           LTV + ++ + +  V Y+L           T+    A N      T  +      +  QS
Sbjct: 181 LTVNDDVVKQELTQVNYKLDAAHPIKNPPETLRRSSASNSQCRPITKTVALEKAIQTEQS 240

Query: 342 WSASLGMELGYTMTIEAGIPFIVEAQIEISGQFSGSYTWGETHSKSTEVEVTHKIEVLPK 401
           W  +  +  G   +I AGIP I  A + +S + S S + G T +K+T   V+  + V P 
Sbjct: 241 WDVTSTVTFGVESSITAGIPDIASATVSVSVETSLSVSLGSTTTKTTTHTVSVIVTVPPN 300

Query: 402 TSVKVGLVATKGYCDVPFSYTRRDVLLNGEARIT 436
               V +VATK   D+PF+        NG+ R T
Sbjct: 301 HYCPVTMVATKYTADIPFTGKMTRTYRNGQKRTT 334

BLAST of Lcy01g008320 vs. ExPASy Swiss-Prot
Match: Q66S25 (Natterin-1 OS=Thalassophryne nattereri OX=289382 PE=1 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 2.8e-05
Identity = 35/141 (24.82%), Postives = 62/141 (43.97%), Query Frame = 0

Query: 282 LTVEEAILSRSIYNVQYRLSEGRIYGENIITMDFQGAMNDSNETNTMGLKFSYLQEESQS 341
           LTV + ++ +++ +V+Y+     +       M      N   +  T  +  S      + 
Sbjct: 167 LTVNKDVIEQTMKDVKYQTEGVTVIQGKPEVMRKSTVNNKQCKEVTKTVTLSKDISTEER 226

Query: 342 WSASLGMELGYTMTIEAGIPFIVEAQIEISGQFSGSYTWGETHSKSTEVEVTHKIEVLPK 401
           W  +  +  G T T+ AGIP +  A + +S +    +  G + ++S    VT  + V PK
Sbjct: 227 WDVTNSVTFGVTTTVTAGIPDVASASLAVSMEARRDFAHGASKTESQSYMVTVSVPVPPK 286

Query: 402 TSVKVGLVATKGYCDVPFSYT 423
            S  V +VA     DVPF+ T
Sbjct: 287 QSCTVSMVAQVNKADVPFTAT 307

BLAST of Lcy01g008320 vs. ExPASy Swiss-Prot
Match: Q66S21 (Natterin-2 OS=Thalassophryne nattereri OX=289382 PE=1 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 3.6e-05
Identity = 34/141 (24.11%), Postives = 64/141 (45.39%), Query Frame = 0

Query: 282 LTVEEAILSRSIYNVQYRLSEGRIYGENIITMDFQGAMNDSNETNTMGLKFSYLQEESQS 341
           LT+ + ++ +++ +V+Y+     +       M      N   +  T  +  +      + 
Sbjct: 168 LTMNKDVIEQAMKDVKYQTEGVTVIKGKPEVMRRSTVNNQHCKEVTKTVTLTKDISTDER 227

Query: 342 WSASLGMELGYTMTIEAGIPFIVEAQIEISGQFSGSYTWGETHSKSTEVEVTHKIEVLPK 401
           W  +  +  G T T+ AGIP +  A +EIS Q +  +  G + +++    VT  + V PK
Sbjct: 228 WDVTNSVTFGVTTTVTAGIPDVSSASLEISMQATMDFAHGASKTETQSYMVTVSVPVPPK 287

Query: 402 TSVKVGLVATKGYCDVPFSYT 423
            S  V +VA     D+PF+ T
Sbjct: 288 QSCTVSMVAQVNKADIPFTAT 308

BLAST of Lcy01g008320 vs. ExPASy TrEMBL
Match: A0A5D3DML5 (Agglutinin domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G003250 PE=4 SV=1)

HSP 1 Score: 643.3 bits (1658), Expect = 7.9e-181
Identity = 303/463 (65.44%), Postives = 364/463 (78.62%), Query Frame = 0

Query: 1   MAVPRFAAFKSSENGKYLKCVGDDDKKLANYVQYSSDELATVYTKFSIEAA-GTGKDHVH 60
           M VPRFA F+SS+N KY+  V  DD+K+ N+VQYSSD LAT  TKFS  A+  T K+  H
Sbjct: 1   MVVPRFAGFRSSKNQKYMNVVKSDDEKIPNFVQYSSDNLATSTTKFSFVASHDTAKNLYH 60

Query: 61  IRSIYNQKYLRAESSSGRWITATAVKPNEDESDWSCTSFRILYSSKSGAYQIIHVHYGNP 120
           I  IYNQKYLRAESSS RWI ATA++PNED+SDW CT FRI + S    Y + H++YG P
Sbjct: 61  IVCIYNQKYLRAESSSSRWICATALEPNEDQSDWGCTLFRITWGSTEQRYDLFHMYYGQP 120

Query: 121 VDLKSDSTHPNCLYIY-TDGTRENHAVLDWEIMAFLPQHVVFKGDNGKYLKIHNQDDHDF 180
           VDLK+DS+HPNCLYIY    T  NH+VLDWE+M+ LPQHVVFKGDN KYLKIHNQD HDF
Sbjct: 121 VDLKNDSSHPNCLYIYDQSSTIYNHSVLDWEVMSILPQHVVFKGDNNKYLKIHNQDSHDF 180

Query: 181 LRFLQTDPHHGSVQHEVSITSDGKLRFKGPNNKFWRAAPHWIWLDSTDTTSNDSYTMFQP 240
           LRFL  DP   SVQ EV ITSDGK+R KGPNNKFWRA+P+WIW+DSTDT+SN+  T+FQP
Sbjct: 181 LRFLLDDPQDSSVQQEVVITSDGKIRIKGPNNKFWRASPNWIWIDSTDTSSNNQATVFQP 240

Query: 241 YKISKNVISLQNLGRNNFCKRNSADWKEDCLCANEGSMSGTTRLTVEEAILSRSIYNVQY 300
            KIS NVI L+NLG N FC R SADWKE CL AN+ +MS  +RLTVEEA+LSR IYNVQY
Sbjct: 241 CKISNNVICLRNLGNNMFCSRTSADWKESCLSANDHAMSNYSRLTVEEAVLSREIYNVQY 300

Query: 301 RLSEGRIYGENIITMDFQGAMNDSNETNTMGLKFSYLQEESQSWSASLGMELGYTMTIEA 360
           RLS+GRIYGENI+ +DFQGAMN S+E N M   FSY +EESQ WS+++GM+L Y+MTIEA
Sbjct: 301 RLSDGRIYGENILAVDFQGAMNGSDEVNHMDFNFSYEEEESQCWSSTVGMKLAYSMTIEA 360

Query: 361 GIPFIVEAQIEISGQFSGSYTWGETHSKSTEVEVTHKIEVLPKTSVKVGLVATKGYCDVP 420
           GIP I++  I +SG+ S SYTWG+T+ KST    ++ +EV P+TSVKV  + TKGYCDVP
Sbjct: 361 GIPLIMDTNIVMSGEISESYTWGQTNKKSTPKRTSYSLEVQPRTSVKVSAMLTKGYCDVP 420

Query: 421 FSYTRRDVLLNGEARITQHDDGIFTGVNCYNFRYDDSDVKSLA 462
           FSYT+RD+L++G+ R T+HDDG+FTG NCYNF Y   DV SL+
Sbjct: 421 FSYTQRDILVDGQTRTTEHDDGLFTGFNCYNFHYQSEDVTSLS 463

BLAST of Lcy01g008320 vs. ExPASy TrEMBL
Match: A0A1S3C8I1 (uncharacterized protein LOC103497997 OS=Cucumis melo OX=3656 GN=LOC103497997 PE=4 SV=1)

HSP 1 Score: 643.3 bits (1658), Expect = 7.9e-181
Identity = 303/463 (65.44%), Postives = 364/463 (78.62%), Query Frame = 0

Query: 1   MAVPRFAAFKSSENGKYLKCVGDDDKKLANYVQYSSDELATVYTKFSIEAA-GTGKDHVH 60
           M VPRFA F+SS+N KY+  V  DD+K+ N+VQYSSD LAT  TKFS  A+  T K+  H
Sbjct: 1   MVVPRFAGFRSSKNQKYMNVVKSDDEKIPNFVQYSSDNLATSTTKFSFVASHDTAKNLYH 60

Query: 61  IRSIYNQKYLRAESSSGRWITATAVKPNEDESDWSCTSFRILYSSKSGAYQIIHVHYGNP 120
           I  IYNQKYLRAESSS RWI ATA++PNED+SDW CT FRI + S    Y + H++YG P
Sbjct: 61  IVCIYNQKYLRAESSSSRWICATALEPNEDQSDWGCTLFRITWGSTEQRYDLFHMYYGQP 120

Query: 121 VDLKSDSTHPNCLYIY-TDGTRENHAVLDWEIMAFLPQHVVFKGDNGKYLKIHNQDDHDF 180
           VDLK+DS+HPNCLYIY    T  NH+VLDWE+M+ LPQHVVFKGDN KYLKIHNQD HDF
Sbjct: 121 VDLKNDSSHPNCLYIYDQSSTIYNHSVLDWEVMSILPQHVVFKGDNNKYLKIHNQDSHDF 180

Query: 181 LRFLQTDPHHGSVQHEVSITSDGKLRFKGPNNKFWRAAPHWIWLDSTDTTSNDSYTMFQP 240
           LRFL  DP   SVQ EV ITSDGK+R KGPNNKFWRA+P+WIW+DSTDT+SN+  T+FQP
Sbjct: 181 LRFLLDDPQDSSVQQEVVITSDGKIRIKGPNNKFWRASPNWIWIDSTDTSSNNQATVFQP 240

Query: 241 YKISKNVISLQNLGRNNFCKRNSADWKEDCLCANEGSMSGTTRLTVEEAILSRSIYNVQY 300
            KIS NVI L+NLG N FC R SADWKE CL AN+ +MS  +RLTVEEA+LSR IYNVQY
Sbjct: 241 CKISNNVICLRNLGNNMFCSRTSADWKESCLSANDHAMSNYSRLTVEEAVLSREIYNVQY 300

Query: 301 RLSEGRIYGENIITMDFQGAMNDSNETNTMGLKFSYLQEESQSWSASLGMELGYTMTIEA 360
           RLS+GRIYGENI+ +DFQGAMN S+E N M   FSY +EESQ WS+++GM+L Y+MTIEA
Sbjct: 301 RLSDGRIYGENILAVDFQGAMNGSDEVNHMDFNFSYEEEESQCWSSTVGMKLAYSMTIEA 360

Query: 361 GIPFIVEAQIEISGQFSGSYTWGETHSKSTEVEVTHKIEVLPKTSVKVGLVATKGYCDVP 420
           GIP I++  I +SG+ S SYTWG+T+ KST    ++ +EV P+TSVKV  + TKGYCDVP
Sbjct: 361 GIPLIMDTNIVMSGEISESYTWGQTNKKSTPKRTSYSLEVQPRTSVKVSAMLTKGYCDVP 420

Query: 421 FSYTRRDVLLNGEARITQHDDGIFTGVNCYNFRYDDSDVKSLA 462
           FSYT+RD+L++G+ R T+HDDG+FTG NCYNF Y   DV SL+
Sbjct: 421 FSYTQRDILVDGQTRTTEHDDGLFTGFNCYNFHYQSEDVTSLS 463

BLAST of Lcy01g008320 vs. ExPASy TrEMBL
Match: A0A6J1DS59 (uncharacterized protein LOC111023023 OS=Momordica charantia OX=3673 GN=LOC111023023 PE=4 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 6.6e-111
Identity = 201/462 (43.51%), Postives = 292/462 (63.20%), Query Frame = 0

Query: 3   VPRFAAFKSSENGKYLKCVGDDDKKLANYVQYSSDELATVYTKFSIEAAGTGKDHVHIRS 62
           +P++   +S  +  YL+ V  +D ++  YV+Y S ++ T YTK+ +E A  GK +V+IR 
Sbjct: 4   LPKYVGLRSLADDLYLRFV-KEDVQIHGYVKYDSHDVVTPYTKYEVEPAKVGKRYVNIRC 63

Query: 63  IYNQKYLRAESSSGRWITATAVKPNEDESDWSCTSFRILYSSKSGAYQIIHVHYG-NPVD 122
            Y+ KY    S + R++ A A +PNEDES+WSCT F  ++ S + AY+I HV  G N   
Sbjct: 64  CYSNKYWVRPSMNSRYVVAAADEPNEDESNWSCTLFEPIHDSGNQAYRIKHVQLGFNTCS 123

Query: 123 LKSDSTHPNCLYIYTDGT-----RENHAVLDWEIMAFLPQHVVFKGDN-GKYLKIHNQDD 182
            +  S H  CL+I T         + H V+DWE     P+HV FKG N GK+LK      
Sbjct: 124 SRDGSIHNECLFIMTSSAPPRNFGDLHIVMDWESFFIFPKHVAFKGGNRGKFLKAIWLQR 183

Query: 183 HDFLRFLQTDPHHGSVQHEVSITSDGKLRFKGPN-NKFWRAAPHWIWLDSTDTTSNDSYT 242
           H++  F   D    +V +EVS T  G +R K  +  KFWR +P+WIW DSTD T+N+ +T
Sbjct: 184 HEYQEFSANDMADPAVGNEVSFTRHGDVRIKSNHFGKFWRLSPNWIWADSTDPTANNKHT 243

Query: 243 MFQPYKISKNVISLQNLGRNNFCKRNSADWKEDCLCANEGSMSGTTRLTVEEAILSRSIY 302
           +F+P K++ N+++L+NLG N+FC   + + K DCL A   +++   R+ + E +LSRSIY
Sbjct: 244 LFKPVKVANNIVALRNLGNNHFCIGLTTEGKTDCLNAWVSTITNEARMEIHELVLSRSIY 303

Query: 303 NVQYRLSEGRIYGENIITMDFQGAMNDSNETNTMGLKFSYLQEESQSWSASLGMELGYTM 362
           NV + L + RIYGENII M    A+N++NE NT+ ++FSY    + +WS++L  +LG   
Sbjct: 304 NVDFHLFDARIYGENIIAMANGNAVNNTNEPNTITIRFSYTNRVTNTWSSTLSAKLGVKA 363

Query: 363 TIEAGIPFIVEAQIEISGQFSGSYTWGETHSKSTEVEVTHKIEVLPKTSVKVGLVATKGY 422
           T  AGIP I + ++ IS +FSG YTWGET   + E+E TH + V P T + V L+AT+GY
Sbjct: 364 TFRAGIPVIAQGRVTISAEFSGEYTWGETRETTDEIETTHTVVVPPMTKMTVSLLATQGY 423

Query: 423 CDVPFSYTRRDVLLNGEARITQHDDGIFTGVNCYNFRYDDSD 457
           CDVPFSY +RDVL+NGE  I  ++DG++TG NCYNF+Y+  D
Sbjct: 424 CDVPFSYYQRDVLMNGETVIELYEDGLYTGANCYNFKYEAKD 464

BLAST of Lcy01g008320 vs. ExPASy TrEMBL
Match: A0A6J1HV52 (uncharacterized protein LOC111467133 OS=Cucurbita maxima OX=3661 GN=LOC111467133 PE=3 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 8.6e-111
Identity = 198/459 (43.14%), Postives = 294/459 (64.05%), Query Frame = 0

Query: 1   MAVPRFAAFKSSENGKYLKCVGDDDKKLANYVQYSSDELATVYTKFSIEAAGTGKDHVHI 60
           M +PR+ AFKS  +  YLK V  +D ++  Y++Y +  + T +TK+ +E A  GK +V+I
Sbjct: 1   MVLPRYVAFKSKADDLYLKYV-KEDVQIHGYIKYDAHNVVTPHTKYEVEPAKVGKGYVNI 60

Query: 61  RSIYNQKYLRAESSSGRWITATAVKPNEDESDWSCTSFRILYSSKSGAYQIIHVHYG-NP 120
           R  YN KY    S + R++ A A +PNED+S+W+CT F  ++     AY+I HV++G N 
Sbjct: 61  RCCYNNKYWVCPSINTRYVVAVADEPNEDQSNWACTLFEPIFDPDHQAYRIKHVYFGYNT 120

Query: 121 VDLKSDSTHPNCLYIYTDGTREN----HAVLDWEIMAFLPQHVVFKGDNGKYLKIHNQDD 180
              +       CLY+       N    H  +DWE    LP+HV FKGDNG YL+    + 
Sbjct: 121 CSWRDGLARDRCLYMGYSTPDANLCDLHIAIDWESFYVLPKHVAFKGDNGSYLQACWIEG 180

Query: 181 HDFLRFLQTDPHHGSVQHEVSITSDGKLRFKGPN-NKFWRAAPHWIWLDSTDTTSNDSYT 240
             +L+F   D    +V +EVS T+DG +R K  +  KFWR + +WIW DS DTT+++  T
Sbjct: 181 FPYLQFSSHDIGDPTVGNEVSTTNDGNVRIKSNHFEKFWRRSSNWIWADSNDTTASNKDT 240

Query: 241 MFQPYKISKNVISLQNLGRNNFCKRNSADWKEDCLCANEGSMSGTTRLTVEEAILSRSIY 300
           +F P ++  N+++L+NLG N+FCKR + + K  CL A   +++   RL ++E +LSRSIY
Sbjct: 241 LFWPVRVENNIVALRNLGNNHFCKRLTTEGKTSCLNAGVSTITKEARLEIQELVLSRSIY 300

Query: 301 NVQYRLSEGRIYGENIITMDFQGAMNDSNETNTMGLKFSYLQEESQSWSASLGMELGYTM 360
           NV + L + RIYG+++ITM    A N SN+ NT+ +K  Y   E+ +WS+++  +LG   
Sbjct: 301 NVIFHLLDARIYGQHVITMANGDASNSSNKENTIDIKLRYTISENNTWSSTVSAKLGVKT 360

Query: 361 TIEAGIPFIVEAQIEISGQFSGSYTWGETHSKSTEVEVTHKIEVLPKTSVKVGLVATKGY 420
           TI+ GIP I + +IE+S +FSG YTWGET SKS+E+E  +K+ V P + V+V L+ATKG+
Sbjct: 361 TIQTGIPLIAKGKIEMSAEFSGGYTWGETRSKSSEIETVYKVTVPPMSRVRVSLLATKGH 420

Query: 421 CDVPFSYTRRDVLLNGEARITQHDDGIFTGVNCYNFRYD 454
           CDVP+SYT+RD L+NG+     +DDGI+T VNCYNF+Y+
Sbjct: 421 CDVPYSYTQRDTLMNGDQVTNYYDDGIYTAVNCYNFKYE 458

BLAST of Lcy01g008320 vs. ExPASy TrEMBL
Match: A0A5B6Z5E6 (Uncharacterized protein OS=Davidia involucrata OX=16924 GN=Din_007674 PE=3 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 5.6e-110
Identity = 201/466 (43.13%), Postives = 296/466 (63.52%), Query Frame = 0

Query: 1   MAVPRFAAFKSSENGKYLKCVGDDDKKLANYVQYSSDELATVYTKFSIEAAGTGKDHVHI 60
           MA+PRF   KS  N KYL  +  +D ++  ++++S +E+ + Y K+ +E A +G   VH+
Sbjct: 1   MALPRFVVLKSKYNDKYLSYI-KEDVQVHGFLRFSGEEVVSPYAKYEVEPAKSGNGLVHM 60

Query: 61  RSIYNQKYLRAESSSGRWITATAVKPNEDESDWSCTSFRILYSSKSGAYQIIHVHYGNPV 120
           R  YN KY    S +  WI A A +P ED+S WSCT F  +Y   +   +  HV  G+  
Sbjct: 61  RCCYNNKYWVRWSQNHWWIVAGADEPEEDQSKWSCTLFEPVYVDAT-TIRFRHVQLGHYA 120

Query: 121 DL-KSDSTHPNCLYIYTDGTRENH----AVLDWEIMAFLPQHVVFKGDNGKYLKIHNQDD 180
            L ++     +C +  ++   ++       +DWE +  LP+HV FKGDNGKYL     ++
Sbjct: 121 CLWRTGDAFDSCSFAGSEAPDKDQCDVCTFIDWESLLILPKHVAFKGDNGKYLAARWTEN 180

Query: 181 HDFLRFLQTDPHHGSVQHEVSITSDGKLRFKGPN-NKFWRAAPHWIWLDSTDTTSNDSYT 240
           H +L+F  +D    +V +EVSIT DG +R K     KFWR +P+WIW DS DT+SN+S T
Sbjct: 181 HPYLQFDSSDIGDPTVGNEVSITRDGSVRIKSDYFGKFWRRSPNWIWADSEDTSSNNSDT 240

Query: 241 MFQPYKISKNVISLQNLGRNNFCKRNSADWKEDCLCANEGSMSGTTRLTVEEAILSRSIY 300
           +F P K+   V++ +NLG NNFCKR + + K +CL A   +++   RL VEE +LSRSIY
Sbjct: 241 LFSPIKVDNKVVAFRNLGNNNFCKRLTTEGKTNCLNAAVSTIASEARLEVEELVLSRSIY 300

Query: 301 NVQYRLSEGRIYGENIITMDFQGAMNDSNETNTMGLKFSYLQEESQSWSASLGMELGYTM 360
           NV YRL + RIY ++++TM    A+ND+NETNT+ +K SY + + ++W+AS+ + LG + 
Sbjct: 301 NVNYRLMDARIYSQSVLTMANGVAVNDTNETNTIEVKLSYTETKGKTWNASVSLTLGVST 360

Query: 361 TIEAGIPFIVEAQIEISGQFSGSYTWGETHSKSTEVEVTHKIEVLPKTSVKVGLVATKGY 420
           +I+AGIP I    IEIS +F+G Y WG T   +T+VE T+ + V P T VKV L+AT+G 
Sbjct: 361 SIQAGIPLIAGGTIEISAEFTGEYQWGSTQESTTQVETTYTVTVPPMTVVKVSLLATQGT 420

Query: 421 CDVPFSYTRRDVLLNGEARITQHDDGIFTGVNCYNFRYDDSDVKSL 461
           CDVPFSYT+RD L NG+   +  DDG++TG+NC+NF+Y+  + K L
Sbjct: 421 CDVPFSYTQRDTLTNGQQTTSTMDDGVYTGINCFNFKYETKEEKLL 464

BLAST of Lcy01g008320 vs. NCBI nr
Match: XP_008458669.1 (PREDICTED: uncharacterized protein LOC103497997 [Cucumis melo] >TYK24762.1 uncharacterized protein E5676_scaffold266G003250 [Cucumis melo var. makuwa])

HSP 1 Score: 643.3 bits (1658), Expect = 1.6e-180
Identity = 303/463 (65.44%), Postives = 364/463 (78.62%), Query Frame = 0

Query: 1   MAVPRFAAFKSSENGKYLKCVGDDDKKLANYVQYSSDELATVYTKFSIEAA-GTGKDHVH 60
           M VPRFA F+SS+N KY+  V  DD+K+ N+VQYSSD LAT  TKFS  A+  T K+  H
Sbjct: 1   MVVPRFAGFRSSKNQKYMNVVKSDDEKIPNFVQYSSDNLATSTTKFSFVASHDTAKNLYH 60

Query: 61  IRSIYNQKYLRAESSSGRWITATAVKPNEDESDWSCTSFRILYSSKSGAYQIIHVHYGNP 120
           I  IYNQKYLRAESSS RWI ATA++PNED+SDW CT FRI + S    Y + H++YG P
Sbjct: 61  IVCIYNQKYLRAESSSSRWICATALEPNEDQSDWGCTLFRITWGSTEQRYDLFHMYYGQP 120

Query: 121 VDLKSDSTHPNCLYIY-TDGTRENHAVLDWEIMAFLPQHVVFKGDNGKYLKIHNQDDHDF 180
           VDLK+DS+HPNCLYIY    T  NH+VLDWE+M+ LPQHVVFKGDN KYLKIHNQD HDF
Sbjct: 121 VDLKNDSSHPNCLYIYDQSSTIYNHSVLDWEVMSILPQHVVFKGDNNKYLKIHNQDSHDF 180

Query: 181 LRFLQTDPHHGSVQHEVSITSDGKLRFKGPNNKFWRAAPHWIWLDSTDTTSNDSYTMFQP 240
           LRFL  DP   SVQ EV ITSDGK+R KGPNNKFWRA+P+WIW+DSTDT+SN+  T+FQP
Sbjct: 181 LRFLLDDPQDSSVQQEVVITSDGKIRIKGPNNKFWRASPNWIWIDSTDTSSNNQATVFQP 240

Query: 241 YKISKNVISLQNLGRNNFCKRNSADWKEDCLCANEGSMSGTTRLTVEEAILSRSIYNVQY 300
            KIS NVI L+NLG N FC R SADWKE CL AN+ +MS  +RLTVEEA+LSR IYNVQY
Sbjct: 241 CKISNNVICLRNLGNNMFCSRTSADWKESCLSANDHAMSNYSRLTVEEAVLSREIYNVQY 300

Query: 301 RLSEGRIYGENIITMDFQGAMNDSNETNTMGLKFSYLQEESQSWSASLGMELGYTMTIEA 360
           RLS+GRIYGENI+ +DFQGAMN S+E N M   FSY +EESQ WS+++GM+L Y+MTIEA
Sbjct: 301 RLSDGRIYGENILAVDFQGAMNGSDEVNHMDFNFSYEEEESQCWSSTVGMKLAYSMTIEA 360

Query: 361 GIPFIVEAQIEISGQFSGSYTWGETHSKSTEVEVTHKIEVLPKTSVKVGLVATKGYCDVP 420
           GIP I++  I +SG+ S SYTWG+T+ KST    ++ +EV P+TSVKV  + TKGYCDVP
Sbjct: 361 GIPLIMDTNIVMSGEISESYTWGQTNKKSTPKRTSYSLEVQPRTSVKVSAMLTKGYCDVP 420

Query: 421 FSYTRRDVLLNGEARITQHDDGIFTGVNCYNFRYDDSDVKSLA 462
           FSYT+RD+L++G+ R T+HDDG+FTG NCYNF Y   DV SL+
Sbjct: 421 FSYTQRDILVDGQTRTTEHDDGLFTGFNCYNFHYQSEDVTSLS 463

BLAST of Lcy01g008320 vs. NCBI nr
Match: XP_023544067.1 (uncharacterized protein LOC111803764 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 419.1 bits (1076), Expect = 5.0e-113
Identity = 202/459 (44.01%), Postives = 300/459 (65.36%), Query Frame = 0

Query: 1   MAVPRFAAFKSSENGKYLKCVGDDDKKLANYVQYSSDELATVYTKFSIEAAGTGKDHVHI 60
           M +PR+ AFKS  +  YLK V  +D ++  Y++Y +  + T YTK+ +E+A  GK +V+I
Sbjct: 1   MVLPRYVAFKSKADDLYLKYV-KEDVQIHGYIKYDAYNVMTPYTKYEVESAKVGKGYVNI 60

Query: 61  RSIYNQKYLRAESSSGRWITATAVKPNEDESDWSCTSFRILYSSKSGAYQIIHVHYG-NP 120
           R  YN KY    S + R++ A A +PNED+S+W+CT F  ++ S   AY+I HV+ G N 
Sbjct: 61  RCCYNNKYWVCPSINTRYVVAVADEPNEDQSNWACTLFEPIFDSDHQAYRIKHVYSGYNT 120

Query: 121 VDLKSDSTHPNCLYI---YTDGTREN-HAVLDWEIMAFLPQHVVFKGDNGKYLKIHNQDD 180
              +  S    CLY+     DG   + H  +DWE    LP++V FK +NG YL+    +D
Sbjct: 121 CSWRDGSVRDRCLYMGYSTPDGNLSDLHIAVDWESFYVLPKYVAFKVENGGYLRAWRFED 180

Query: 181 HDFLRFLQTDPHHGSVQHEVSITSDGKLRFKGPN-NKFWRAAPHWIWLDSTDTTSNDSYT 240
           H +L+F   D    +V +EVS T DG +R K     KFWR +P+WIW DS DTT+++  T
Sbjct: 181 HPYLQFSANDIGDPTVGNEVSTTHDGNVRIKSNYFGKFWRRSPNWIWADSHDTTASNKDT 240

Query: 241 MFQPYKISKNVISLQNLGRNNFCKRNSADWKEDCLCANEGSMSGTTRLTVEEAILSRSIY 300
           +F P K+  N+++L+NLG N+FCKR S D K +CL A+  +++   R+ ++E +LSRSIY
Sbjct: 241 LFWPVKVENNIVALRNLGNNHFCKRLSMDDKTNCLNADVPTITNEARMEIQELVLSRSIY 300

Query: 301 NVQYRLSEGRIYGENIITMDFQGAMNDSNETNTMGLKFSYLQEESQSWSASLGMELGYTM 360
           NV + L + RIYG+++ITM    A ND+N+ NT+G+K  Y   ES +W++++  +LG   
Sbjct: 301 NVIFHLLDARIYGQHVITMTSGDASNDTNKENTVGMKLHYTVSESNTWTSTISAKLGIQT 360

Query: 361 TIEAGIPFIVEAQIEISGQFSGSYTWGETHSKSTEVEVTHKIEVLPKTSVKVGLVATKGY 420
           +I+ GIP I E +IE+S +FSG YTWGET S S+E+E  + + V P + V+V L+ATKG+
Sbjct: 361 SIQTGIPLIAEGKIEMSAEFSGEYTWGETKSNSSEIETVYTVTVPPMSRVRVSLLATKGH 420

Query: 421 CDVPFSYTRRDVLLNGEARITQHDDGIFTGVNCYNFRYD 454
           CD+P+SYT+RD L+NG+     +DDGI+T VNCYNF+Y+
Sbjct: 421 CDIPYSYTQRDTLMNGDQVTNYYDDGIYTAVNCYNFKYE 458

BLAST of Lcy01g008320 vs. NCBI nr
Match: XP_023544075.1 (uncharacterized protein LOC111803769 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 419.1 bits (1076), Expect = 5.0e-113
Identity = 204/459 (44.44%), Postives = 295/459 (64.27%), Query Frame = 0

Query: 1   MAVPRFAAFKSSENGKYLKCVGDDDKKLANYVQYSSDELATVYTKFSIEAAGTGKDHVHI 60
           M +PR+ AFKS  +  YLK V  +D ++  Y++Y +  + T +TK+ +E A  GK +V+I
Sbjct: 1   MVLPRYVAFKSKADDLYLKYV-KEDVQIHGYIKYDAHNVMTPHTKYEVEPAKVGKGYVNI 60

Query: 61  RSIYNQKYLRAESSSGRWITATAVKPNEDESDWSCTSFRILYSSKSGAYQIIHVHYG-NP 120
           R  YN KY    S + R++ A A +PNED+S+W+CT F  ++     AY+I HV+ G N 
Sbjct: 61  RCCYNNKYWVCPSINTRYVVAVADEPNEDQSNWACTLFEPIFDPDHQAYRIKHVYLGYNT 120

Query: 121 VDLKSDSTHPNCLYIYTDGTREN----HAVLDWEIMAFLPQHVVFKGDNGKYLKIHNQDD 180
              +   T   CLY+       N    H  +DWE    LP++V FKGDNG YL+    + 
Sbjct: 121 CSWRDGLTRDKCLYMGYSTPDANLCDLHVAIDWESFYVLPKYVAFKGDNGSYLQACWVEG 180

Query: 181 HDFLRFLQTDPHHGSVQHEVSITSDGKLRFKGPN-NKFWRAAPHWIWLDSTDTTSNDSYT 240
             +L+F   D    +V +EVS T+DG +R K  +  KFWR +P+WIW DS DTT+ND  T
Sbjct: 181 FPYLQFSSNDIGDPTVGNEVSTTNDGNVRIKSNHFEKFWRRSPNWIWADSHDTTANDKDT 240

Query: 241 MFQPYKISKNVISLQNLGRNNFCKRNSADWKEDCLCANEGSMSGTTRLTVEEAILSRSIY 300
           +F P K+  N+++L+NLG N+FCKR + + K  CL A   +++   RL ++E +LSRSIY
Sbjct: 241 LFWPVKVDNNIVALRNLGNNHFCKRLTTEGKTSCLNAGVPTITKEARLEIQELVLSRSIY 300

Query: 301 NVQYRLSEGRIYGENIITMDFQGAMNDSNETNTMGLKFSYLQEESQSWSASLGMELGYTM 360
           NV + L + RIYG+++ITM    A N SN+ NT+ +K  Y   ES +WS+++  +LG   
Sbjct: 301 NVIFHLLDARIYGQHVITMANGDASNSSNKENTIDIKLRYTISESNTWSSTVSAKLGVKT 360

Query: 361 TIEAGIPFIVEAQIEISGQFSGSYTWGETHSKSTEVEVTHKIEVLPKTSVKVGLVATKGY 420
           TI+ GIP I E +IE+S +FSG YTWGET SKS+E+E  +K+ V P + V+V L+ATKG+
Sbjct: 361 TIQTGIPLIAEGKIEMSAEFSGGYTWGETQSKSSEIETVYKVTVPPMSRVRVSLLATKGH 420

Query: 421 CDVPFSYTRRDVLLNGEARITQHDDGIFTGVNCYNFRYD 454
           CDVP+SYT+RD L+NG+     +DDGI+T VNCYNF+Y+
Sbjct: 421 CDVPYSYTQRDTLMNGDQVTNYYDDGIYTAVNCYNFKYE 458

BLAST of Lcy01g008320 vs. NCBI nr
Match: KAG6603266.1 (hypothetical protein SDJN03_03875, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 414.1 bits (1063), Expect = 1.6e-111
Identity = 202/459 (44.01%), Postives = 293/459 (63.83%), Query Frame = 0

Query: 1   MAVPRFAAFKSSENGKYLKCVGDDDKKLANYVQYSSDELATVYTKFSIEAAGTGKDHVHI 60
           M  PR+ AFKS  +  YLK V  +D ++  Y++Y +  + T +TK+ +E A  GK +V+I
Sbjct: 1   MVFPRYVAFKSKADDLYLKYV-KEDVQIHGYIKYDAHNVMTPHTKYEVEPAKVGKGYVNI 60

Query: 61  RSIYNQKYLRAESSSGRWITATAVKPNEDESDWSCTSFRILYSSKSGAYQIIHVHYG-NP 120
           R  YN KY    S + R++ A A +PNED+S+W+CT F  ++     AY+I HV+ G N 
Sbjct: 61  RCCYNNKYWVCPSINTRYVVAVADEPNEDQSNWACTLFEPIFDPDHQAYRIKHVYLGYNT 120

Query: 121 VDLKSDSTHPNCLYIYTDGTREN----HAVLDWEIMAFLPQHVVFKGDNGKYLKIHNQDD 180
              +   T   CLY+       N    H  +DWE    LP++V FKGDNG YL+    + 
Sbjct: 121 CSWRDGLTRDKCLYMGYSTPDANLCDLHIAIDWESFYVLPKYVAFKGDNGSYLQACWIEG 180

Query: 181 HDFLRFLQTDPHHGSVQHEVSITSDGKLRFKGPN-NKFWRAAPHWIWLDSTDTTSNDSYT 240
             +L+F   D    +V +EVS T+DG +R K  +  KFWR +P+WIW DS DTT+N+  T
Sbjct: 181 FPYLQFSSNDIGDPTVGNEVSTTNDGNVRIKSNHFEKFWRRSPNWIWADSNDTTANNKDT 240

Query: 241 MFQPYKISKNVISLQNLGRNNFCKRNSADWKEDCLCANEGSMSGTTRLTVEEAILSRSIY 300
           +F P K+  N+++L+N G N+FCKR + + K  CL A   +++   RL ++E +LSRSIY
Sbjct: 241 LFWPVKVDNNIVALRNSGNNHFCKRLTTEGKTSCLNAGVPTITKEARLEIQELVLSRSIY 300

Query: 301 NVQYRLSEGRIYGENIITMDFQGAMNDSNETNTMGLKFSYLQEESQSWSASLGMELGYTM 360
           NV + L + RIYG+++ITM    A N SN+ NT+ +K  Y   ES +WS+++  +LG   
Sbjct: 301 NVIFHLLDARIYGQHVITMANGDASNSSNKENTIDIKLRYTISESNTWSSTVSAKLGVKT 360

Query: 361 TIEAGIPFIVEAQIEISGQFSGSYTWGETHSKSTEVEVTHKIEVLPKTSVKVGLVATKGY 420
           TI+ GIP I E +IE+S +FSG YTWGET SKS+E+E  +K+ V P + V+V L+ATKG+
Sbjct: 361 TIQTGIPLIAEGKIEMSAEFSGGYTWGETQSKSSEIETVYKVTVPPMSRVRVSLLATKGH 420

Query: 421 CDVPFSYTRRDVLLNGEARITQHDDGIFTGVNCYNFRYD 454
           CDVP+SYT+RD L+NG+     +DDGI+T VNCYNF+Y+
Sbjct: 421 CDVPYSYTQRDTLMNGDQVTNYYDDGIYTAVNCYNFKYE 458

BLAST of Lcy01g008320 vs. NCBI nr
Match: XP_022156054.1 (uncharacterized protein LOC111023023 [Momordica charantia])

HSP 1 Score: 411.0 bits (1055), Expect = 1.4e-110
Identity = 201/462 (43.51%), Postives = 292/462 (63.20%), Query Frame = 0

Query: 3   VPRFAAFKSSENGKYLKCVGDDDKKLANYVQYSSDELATVYTKFSIEAAGTGKDHVHIRS 62
           +P++   +S  +  YL+ V  +D ++  YV+Y S ++ T YTK+ +E A  GK +V+IR 
Sbjct: 4   LPKYVGLRSLADDLYLRFV-KEDVQIHGYVKYDSHDVVTPYTKYEVEPAKVGKRYVNIRC 63

Query: 63  IYNQKYLRAESSSGRWITATAVKPNEDESDWSCTSFRILYSSKSGAYQIIHVHYG-NPVD 122
            Y+ KY    S + R++ A A +PNEDES+WSCT F  ++ S + AY+I HV  G N   
Sbjct: 64  CYSNKYWVRPSMNSRYVVAAADEPNEDESNWSCTLFEPIHDSGNQAYRIKHVQLGFNTCS 123

Query: 123 LKSDSTHPNCLYIYTDGT-----RENHAVLDWEIMAFLPQHVVFKGDN-GKYLKIHNQDD 182
            +  S H  CL+I T         + H V+DWE     P+HV FKG N GK+LK      
Sbjct: 124 SRDGSIHNECLFIMTSSAPPRNFGDLHIVMDWESFFIFPKHVAFKGGNRGKFLKAIWLQR 183

Query: 183 HDFLRFLQTDPHHGSVQHEVSITSDGKLRFKGPN-NKFWRAAPHWIWLDSTDTTSNDSYT 242
           H++  F   D    +V +EVS T  G +R K  +  KFWR +P+WIW DSTD T+N+ +T
Sbjct: 184 HEYQEFSANDMADPAVGNEVSFTRHGDVRIKSNHFGKFWRLSPNWIWADSTDPTANNKHT 243

Query: 243 MFQPYKISKNVISLQNLGRNNFCKRNSADWKEDCLCANEGSMSGTTRLTVEEAILSRSIY 302
           +F+P K++ N+++L+NLG N+FC   + + K DCL A   +++   R+ + E +LSRSIY
Sbjct: 244 LFKPVKVANNIVALRNLGNNHFCIGLTTEGKTDCLNAWVSTITNEARMEIHELVLSRSIY 303

Query: 303 NVQYRLSEGRIYGENIITMDFQGAMNDSNETNTMGLKFSYLQEESQSWSASLGMELGYTM 362
           NV + L + RIYGENII M    A+N++NE NT+ ++FSY    + +WS++L  +LG   
Sbjct: 304 NVDFHLFDARIYGENIIAMANGNAVNNTNEPNTITIRFSYTNRVTNTWSSTLSAKLGVKA 363

Query: 363 TIEAGIPFIVEAQIEISGQFSGSYTWGETHSKSTEVEVTHKIEVLPKTSVKVGLVATKGY 422
           T  AGIP I + ++ IS +FSG YTWGET   + E+E TH + V P T + V L+AT+GY
Sbjct: 364 TFRAGIPVIAQGRVTISAEFSGEYTWGETRETTDEIETTHTVVVPPMTKMTVSLLATQGY 423

Query: 423 CDVPFSYTRRDVLLNGEARITQHDDGIFTGVNCYNFRYDDSD 457
           CDVPFSY +RDVL+NGE  I  ++DG++TG NCYNF+Y+  D
Sbjct: 424 CDVPFSYYQRDVLMNGETVIELYEDGLYTGANCYNFKYEAKD 464