Lcy01g008070 (gene) Sponge gourd (P93075) v1

Overview
NameLcy01g008070
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRNase H domain-containing protein
LocationChr01: 16536272 .. 16536855 (-)
RNA-Seq ExpressionLcy01g008070
SyntenyLcy01g008070
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTTAAGTAAATAACTGAATTACACTGAAATATTAATTATATATATTATCAAAAAGCATCTTAAAATCATATGTAATGAATGTCATACTAGGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTGATTGGGTAGGGGTCTGGGCTGGGTGCTGCGGGATGTTACTGGAGATGTGCTCGAGGCAGGTTGTGAACCGGTCACGGGACCCCTTGGGGTGGTTATGTTGGAAATGCAGGCGATTCGAGCTGGATTGTTGGTTGTTGGTGGGATGAGGCTTCCTCGCCTATTGGTGGAATCAGATTGTCTGACTATTATAAATCTTTTAAATGGTGAGGAGGAAAACTTCACCTTAGTCCGGTCAATTGCTATGGAGGTGCAGCGACTTTCTTCCCGTTTGGTGGGAACACAGTTTAAGCATATTCAGCGAGGCCAAAATGTGGTGGCTGACTTGTTGGCGCAACGAGCAAGGATTAATGGAGATCATGGTTCTTGGTTTTCAGGTTTCCCAAGTTGGCTACTGGAAGCGATAGATGGTGACAAACGATGTTTAAACTTATTGTAA

mRNA sequence

ATGCTTAAGGGTCTGGGCTGGGTGCTGCGGGATGTTACTGGAGATGTGCTCGAGGCAGGTTGTGAACCGGTCACGGGACCCCTTGGGGTGGTTATGTTGGAAATGCAGGCGATTCGAGCTGGATTGTTGGTTGTTGGTGGGATGAGGCTTCCTCGCCTATTGGTGGAATCAGATTGTCTGACTATTATAAATCTTTTAAATGGTGAGGAGGAAAACTTCACCTTAGTCCGGTCAATTGCTATGGAGGTGCAGCGACTTTCTTCCCGTTTGGTGGGAACACAGTTTAAGCATATTCAGCGAGGCCAAAATGTGGTGGCTGACTTGTTGGCGCAACGAGCAAGGATTAATGGAGATCATGGTTCTTGGTTTTCAGGTTTCCCAAGTTGGCTACTGGAAGCGATAGATGGTGACAAACGATGTTTAAACTTATTGTAA

Coding sequence (CDS)

ATGCTTAAGGGTCTGGGCTGGGTGCTGCGGGATGTTACTGGAGATGTGCTCGAGGCAGGTTGTGAACCGGTCACGGGACCCCTTGGGGTGGTTATGTTGGAAATGCAGGCGATTCGAGCTGGATTGTTGGTTGTTGGTGGGATGAGGCTTCCTCGCCTATTGGTGGAATCAGATTGTCTGACTATTATAAATCTTTTAAATGGTGAGGAGGAAAACTTCACCTTAGTCCGGTCAATTGCTATGGAGGTGCAGCGACTTTCTTCCCGTTTGGTGGGAACACAGTTTAAGCATATTCAGCGAGGCCAAAATGTGGTGGCTGACTTGTTGGCGCAACGAGCAAGGATTAATGGAGATCATGGTTCTTGGTTTTCAGGTTTCCCAAGTTGGCTACTGGAAGCGATAGATGGTGACAAACGATGTTTAAACTTATTGTAA

Protein sequence

MLKGLGWVLRDVTGDVLEAGCEPVTGPLGVVMLEMQAIRAGLLVVGGMRLPRLLVESDCLTIINLLNGEEENFTLVRSIAMEVQRLSSRLVGTQFKHIQRGQNVVADLLAQRARINGDHGSWFSGFPSWLLEAIDGDKRCLNLL
Homology
BLAST of Lcy01g008070 vs. ExPASy TrEMBL
Match: B8AFN2 (RNase H domain-containing protein OS=Oryza sativa subsp. indica OX=39946 GN=OsI_06781 PE=4 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 8.3e-12
Identity = 45/134 (33.58%), Postives = 75/134 (55.97%), Query Frame = 0

Query: 4   GLGWVLRDVTGDVLEAGCEPVTGPLGVVMLEMQAIRAGLLVVGGMRLPRLLVESDCLTII 63
           G+G VLRD +G+ + A C+P+    G +  E++A   GL +V    L  +LVE+DC++++
Sbjct: 181 GIGMVLRDSSGNTIFASCKPLNRCTGALESELRACVEGLNLVLHWTLLPILVETDCMSVV 240

Query: 64  NLLNGEEENFTLVRSIAMEVQRLSSRLVGTQFKHIQRGQNVVADLLAQRARINGDHGSWF 123
            LLN  E + + + +I  E +RL +         I R QN V+ LLA RA+++   G W 
Sbjct: 241 QLLNDGERDRSELANIVHEAKRLLAGTRRILVSKIHRSQNSVSHLLANRAKVDSVSGVWL 300

Query: 124 SGFPSWLLEAIDGD 138
            G  +++L  +  D
Sbjct: 301 EGSCNFILRLVRED 314

BLAST of Lcy01g008070 vs. ExPASy TrEMBL
Match: A0A7N2N656 (RNase H domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 5.4e-11
Identity = 47/136 (34.56%), Postives = 69/136 (50.74%), Query Frame = 0

Query: 2   LKGLGWVLRDVTGDVLEAGCEPVTGPLGVVMLEMQAIRAGLLVVGGMRLPRLLVESDCLT 61
           L GLG VLRD  G+V+ A  E +  P  V + E  A R  ++    + + +++VE DCL 
Sbjct: 34  LAGLGVVLRDSGGNVIAALSEKIKLPQTVELAEALAARRAVIFAAELSIFKVMVEGDCLR 93

Query: 62  IINLLNGEEENFTLVRSIAMEVQRLSSRLVGTQFKHIQRGQNVVADLLAQRARINGDHGS 121
           +I  LNG+    T+   +  E +RL S L    F H++R  N +A  LA+RA ++ D   
Sbjct: 94  VIQALNGQGSCLTMFGHVVEETRRLGSCLELCSFHHVKREGNKLAHSLARRAVLSADVDV 153

Query: 122 WFSGFPSWLLEAIDGD 138
           W    P  L     GD
Sbjct: 154 WVEEQPEDLDAVFQGD 169

BLAST of Lcy01g008070 vs. ExPASy TrEMBL
Match: A0A7N2LIP9 (RNase H domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 7.0e-11
Identity = 45/136 (33.09%), Postives = 70/136 (51.47%), Query Frame = 0

Query: 2   LKGLGWVLRDVTGDVLEAGCEPVTGPLGVVMLEMQAIRAGLLVVGGMRLPRLLVESDCLT 61
           L GLG V+RD  G+V+ A  E +  P  V + E  A R  +++   + + +++VE DCL 
Sbjct: 34  LAGLGVVIRDSGGNVIAALSEKIKLPQSVELAEALAARRAVILAAELSIFKVIVEGDCLR 93

Query: 62  IINLLNGEEENFTLVRSIAMEVQRLSSRLVGTQFKHIQRGQNVVADLLAQRARINGDHGS 121
           +I  L+G+    T+   +A E +RL   L    F H++R  N +A  LA+RA ++ D   
Sbjct: 94  VIQALSGQGSCLTMFGHVAEETRRLGLCLELCSFHHVKRDGNKLAHSLARRAVLSADVDV 153

Query: 122 WFSGFPSWLLEAIDGD 138
           W    P  L     GD
Sbjct: 154 WVEELPEDLDAVFQGD 169

BLAST of Lcy01g008070 vs. ExPASy TrEMBL
Match: A0A7N2LIC5 (RNase H domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 9.2e-11
Identity = 46/136 (33.82%), Postives = 69/136 (50.74%), Query Frame = 0

Query: 2   LKGLGWVLRDVTGDVLEAGCEPVTGPLGVVMLEMQAIRAGLLVVGGMRLPRLLVESDCLT 61
           L GLG VLRD  G+V+ A  E +  P  V + E  A R  ++    + + +++VE DCL 
Sbjct: 34  LAGLGVVLRDSGGNVIAALSEKIKLPQTVELAEALAARRAVIFAAELSIFKVMVEGDCLR 93

Query: 62  IINLLNGEEENFTLVRSIAMEVQRLSSRLVGTQFKHIQRGQNVVADLLAQRARINGDHGS 121
           +I  LNG+    T+   +  E +RL S L    F H++R  N +A  LA+RA ++ +   
Sbjct: 94  VIQALNGQGSCLTMFGHVVEETRRLGSCLELCSFHHVKREGNKLAHSLARRAVLSANVDV 153

Query: 122 WFSGFPSWLLEAIDGD 138
           W    P  L     GD
Sbjct: 154 WVEELPEDLDAVFQGD 169

BLAST of Lcy01g008070 vs. ExPASy TrEMBL
Match: A0A7N2L9Q3 (RNase H domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 4.6e-10
Identity = 46/136 (33.82%), Postives = 67/136 (49.26%), Query Frame = 0

Query: 2   LKGLGWVLRDVTGDVLEAGCEPVTGPLGVVMLEMQAIRAGLLVVGGMRLPRLLVESDCLT 61
           L GLG VLRD  G ++ A  E +  P  V + E  A R  +L    + + +++VE DCL 
Sbjct: 70  LAGLGVVLRDSGGHIIAALSEKIKLPQTVELAEALAARRAVLFATELSIFKVMVEGDCLR 129

Query: 62  IINLLNGEEENFTLVRSIAMEVQRLSSRLVGTQFKHIQRGQNVVADLLAQRARINGDHGS 121
           +I  LN +    T+   +  E +RL S L    F H++R  N +A  LA+RA ++ D   
Sbjct: 130 VIQALNEQGSCLTMFGHVVEETRRLGSCLELCSFHHVKREGNKLAHSLARRAVLSADVDV 189

Query: 122 WFSGFPSWLLEAIDGD 138
           W    P  L     GD
Sbjct: 190 WVEELPEDLDAVFQGD 205

BLAST of Lcy01g008070 vs. NCBI nr
Match: EEC72931.1 (hypothetical protein OsI_06781 [Oryza sativa Indica Group])

HSP 1 Score: 80.1 bits (196), Expect = 1.7e-11
Identity = 45/134 (33.58%), Postives = 75/134 (55.97%), Query Frame = 0

Query: 4   GLGWVLRDVTGDVLEAGCEPVTGPLGVVMLEMQAIRAGLLVVGGMRLPRLLVESDCLTII 63
           G+G VLRD +G+ + A C+P+    G +  E++A   GL +V    L  +LVE+DC++++
Sbjct: 181 GIGMVLRDSSGNTIFASCKPLNRCTGALESELRACVEGLNLVLHWTLLPILVETDCMSVV 240

Query: 64  NLLNGEEENFTLVRSIAMEVQRLSSRLVGTQFKHIQRGQNVVADLLAQRARINGDHGSWF 123
            LLN  E + + + +I  E +RL +         I R QN V+ LLA RA+++   G W 
Sbjct: 241 QLLNDGERDRSELANIVHEAKRLLAGTRRILVSKIHRSQNSVSHLLANRAKVDSVSGVWL 300

Query: 124 SGFPSWLLEAIDGD 138
            G  +++L  +  D
Sbjct: 301 EGSCNFILRLVRED 314

BLAST of Lcy01g008070 vs. NCBI nr
Match: KAG2726715.1 (hypothetical protein I3760_01G126500 [Carya illinoinensis])

HSP 1 Score: 74.3 bits (181), Expect = 9.4e-10
Identity = 50/131 (38.17%), Postives = 67/131 (51.15%), Query Frame = 0

Query: 4   GLGWVLRDVTGDVLEAGCEPVTGPLGVVMLEMQAIRAGLLVVGGMRLPRLLVESDCLTII 63
           GLG VLRD TG VL A  +P     G   LE+ AI  GL +   M +P++LVESD L  I
Sbjct: 276 GLGAVLRDHTGKVLMAVSQPALEVGGSEPLELMAIFRGLQMCATMGIPKILVESDSLLCI 335

Query: 64  NLLNGEEENFTLVRSIAMEVQRLSSRLVGTQFKHIQRGQNVVADLLAQRARINGDHGSWF 123
             L     + +L+  + ME+Q L SR V   F H+ R  N VA  LA+ A        W+
Sbjct: 336 EGLQYNNMHQSLLGGLYMEIQSLVSRFVCCDFSHVYREGNQVAHRLARFASSIVSIAVWW 395

Query: 124 SGFPSWLLEAI 135
              P  + +A+
Sbjct: 396 DCIPDCISQAV 406

BLAST of Lcy01g008070 vs. NCBI nr
Match: XP_030945898.1 (uncharacterized protein LOC115970400 [Quercus lobata])

HSP 1 Score: 73.9 bits (180), Expect = 1.2e-09
Identity = 47/136 (34.56%), Postives = 66/136 (48.53%), Query Frame = 0

Query: 2   LKGLGWVLRDVTGDVLEAGCEPVTGPLGVVMLEMQAIRAGLLVVGGMRLPRLLVESDCLT 61
           L GLG VLRD  G ++ A  E +  P  V + E  A R  +L      + +++VE DCL 
Sbjct: 39  LAGLGVVLRDSGGHIIAALSEKIKLPQTVELAEALAARRAVLFATEFSIFKVMVEGDCLR 98

Query: 62  IINLLNGEEENFTLVRSIAMEVQRLSSRLVGTQFKHIQRGQNVVADLLAQRARINGDHGS 121
           +I  LN +    T+   +A E +RL S L    F H++R  N +A  LA+RA +  D   
Sbjct: 99  VIQALNEQGSCLTMFGHVAEETRRLGSCLELCSFHHVKREGNKLAHSLARRAVLPADVDV 158

Query: 122 WFSGFPSWLLEAIDGD 138
           W    P  L     GD
Sbjct: 159 WVEELPEDLDAVFQGD 174

BLAST of Lcy01g008070 vs. NCBI nr
Match: XP_023902041.1 (uncharacterized protein LOC112013897 [Quercus suber])

HSP 1 Score: 72.0 bits (175), Expect = 4.7e-09
Identity = 43/135 (31.85%), Postives = 68/135 (50.37%), Query Frame = 0

Query: 5   LGWVLRDVTGDVLEAGCEPVTGPLGVVMLEMQAIRAGLLVVGGMRLPRLLVESDCLTIIN 64
           +G ++RD TGDV  A C    G   V  +E  A+  GLL+    +LP++++ESD L +++
Sbjct: 811 VGVIIRDATGDVCAACCTYFQGQYSVEEVEALAMERGLLLAKEQKLPQIIMESDALAVVS 870

Query: 65  LLNGEEENFTLVRSIAMEVQRLSSRLVGTQFKHIQRGQNVVADLLAQRARINGDHGSWFS 124
            +N  E +  L   I   +  L S     + KH++R  N  A  LAQ AR+  D   W  
Sbjct: 871 KVNSAETSGCL-GHIYQGILSLLSSFSCWKIKHVKREYNKAAHELAQYARLKEDSQVWIG 930

Query: 125 GFPSWLLEAIDGDKR 140
             P  +++ +  D R
Sbjct: 931 VCPPMVMQLVQEDGR 944

BLAST of Lcy01g008070 vs. NCBI nr
Match: XP_023916941.1 (uncharacterized protein LOC112028476 [Quercus suber])

HSP 1 Score: 72.0 bits (175), Expect = 4.7e-09
Identity = 44/136 (32.35%), Postives = 68/136 (50.00%), Query Frame = 0

Query: 2   LKGLGWVLRDVTGDVLEAGCEPVTGPLGVVMLEMQAIRAGLLVVGGMRLPRLLVESDCLT 61
           L GLG V+RD +G ++ A  + +  P  V M+E    R  L+    + + +  VE D L 
Sbjct: 91  LAGLGIVIRDESGLIIAALSQKIPLPRSVDMVEALDARQALIFAQEISIFKAEVEGDSLN 150

Query: 62  IINLLNGEEENFTLVRSIAMEVQRLSSRLVGTQFKHIQRGQNVVADLLAQRARINGDHGS 121
           +I  LN  + N TL+  I  ++Q L + +    F H +RG N++A  LAQRA +  D   
Sbjct: 151 VIQALNNPKPNRTLMGHIISDIQCLGAAMQKCDFMHTRRGGNMLAHSLAQRAVLAADTDV 210

Query: 122 WFSGFPSWLLEAIDGD 138
           W    P  L++    D
Sbjct: 211 WLEELPQDLVDIFQFD 226

BLAST of Lcy01g008070 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 63.9 bits (154), Expect = 1.2e-10
Identity = 39/128 (30.47%), Postives = 67/128 (52.34%), Query Frame = 0

Query: 4   GLGWVLRDVTGDVLEAGCEPVTGPLGVVMLEMQAIRAGLLVVGGMRLPRLLVESDCLTII 63
           G+GW+LR+ +G VL  G   +     V+  E++A+R  +L +      R++ ESD   ++
Sbjct: 161 GIGWILRNESGGVLWMGARALPRTKNVLEAELEALRWAVLTMSRFNYKRIIFESDAQALV 220

Query: 64  NLLNGEEENFTLVRSIAMEVQRLSSRLVGTQFKHIQRGQNVVADLLAQRA-RINGDHGSW 123
           NLLN ++   TL  ++  ++Q+L       +F+   RG N VAD +A+ +   +      
Sbjct: 221 NLLNSDDFWPTLQPALE-DIQQLLHHFEEVKFEFTPRGGNKVADRIARESISFSNYDPKL 280

Query: 124 FSGFPSWL 131
           FS  P WL
Sbjct: 281 FSIVPQWL 287

BLAST of Lcy01g008070 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 55.1 bits (131), Expect = 5.5e-08
Identity = 38/134 (28.36%), Postives = 70/134 (52.24%), Query Frame = 0

Query: 4   GLGWVLRDVTGDVLEAGCEPVTGPLGVVMLEMQAIRAGLLVVGGMRLPRLLVESDCLTII 63
           G+GWVLR+  G+V   G   +     V+  E++A+R  +L +   +   ++ ESD   +I
Sbjct: 444 GIGWVLRNEKGEVKWMGARALPKLKSVLEAELEAMRWAVLSLSRFQYNYVIFESDSQVLI 503

Query: 64  NLLNGEEENFTLVRSIAMEVQRLSSRLVGTQFKHIQRGQNVVADLLAQRAR--INGDHGS 123
            +LN  +E +  ++    ++QRL S+    +F  I R  N +A+ +A+ +   +N D   
Sbjct: 504 EILN-NDEIWPSLKPTIQDLQRLLSQFTEVKFVFIPREGNTLAERVARESLSFLNYD-PK 563

Query: 124 WFSGFPSWLLEAID 136
            +S  PSW   ++D
Sbjct: 564 LYSIVPSWARSSMD 575

BLAST of Lcy01g008070 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 45.1 bits (105), Expect = 5.7e-05
Identity = 31/125 (24.80%), Postives = 56/125 (44.80%), Query Frame = 0

Query: 6   GWVLRDVTGDVLEAGCEPVTGPLGVVMLEMQAIRAGLLVVGGMRLPRLLVESDCLTIINL 65
           GW++R+  G  +  G   +      +  E +A+ A L         ++ +E DC T+INL
Sbjct: 352 GWIIRNHYGTPISWGSMKLAHTSNPLEAETKALLAALQQTWIRGYTQVFMEGDCQTLINL 411

Query: 66  LNGEEENFTLVRSIAMEVQRLSSRLVGTQFKHIQRGQNVVADLLAQRARINGDHGSWFSG 125
           +NG   + +L   +  ++   +++    QF  I+R  N +A +LA+         S    
Sbjct: 412 INGISFHSSLANHLE-DISFWANKFASIQFGFIRRKGNKLAHVLAKYGCTYSTFYSGSGS 471

Query: 126 FPSWL 131
            P WL
Sbjct: 472 LPIWL 475

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
B8AFN28.3e-1233.58RNase H domain-containing protein OS=Oryza sativa subsp. indica OX=39946 GN=OsI_... [more]
A0A7N2N6565.4e-1134.56RNase H domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1[more]
A0A7N2LIP97.0e-1133.09RNase H domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1[more]
A0A7N2LIC59.2e-1133.82RNase H domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1[more]
A0A7N2L9Q34.6e-1033.82RNase H domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
EEC72931.11.7e-1133.58hypothetical protein OsI_06781 [Oryza sativa Indica Group][more]
KAG2726715.19.4e-1038.17hypothetical protein I3760_01G126500 [Carya illinoinensis][more]
XP_030945898.11.2e-0934.56uncharacterized protein LOC115970400 [Quercus lobata][more]
XP_023902041.14.7e-0931.85uncharacterized protein LOC112013897 [Quercus suber][more]
XP_023916941.14.7e-0932.35uncharacterized protein LOC112028476 [Quercus suber][more]
Match NameE-valueIdentityDescription
AT2G34320.11.2e-1030.47Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT4G29090.15.5e-0828.36Ribonuclease H-like superfamily protein [more]
AT3G09510.15.7e-0524.80Ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 4..113
e-value: 8.5E-21
score: 74.0
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 2..114
e-value: 1.1E-10
score: 43.7
NoneNo IPR availablePANTHERPTHR33033:SF67SUBFAMILY NOT NAMEDcoord: 4..134
NoneNo IPR availablePANTHERPTHR33033POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 4..134
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 4..110
e-value: 1.29777E-16
score: 68.88
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 4..114

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy01g008070.1Lcy01g008070.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity