Lcy01g007140 (gene) Sponge gourd (P93075) v1

Overview
NameLcy01g007140
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptionsphingoid long-chain bases kinase 2, mitochondrial
LocationChr01: 11679870 .. 11681359 (-)
RNA-Seq ExpressionLcy01g007140
SyntenyLcy01g007140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCTATTTGTTAACACAAAAATCGCATTAGCATTGGTAGTTCATGTAAGATATTTTGCAGGGTTGAGGTGCCGAATCGATGTTGGAGTTGTCAATGAAGAAAATGGACAGGATCATTACTTCGCAAATGTTGCAGACATTCATTTGTAAGGTTAATCTAAACAATTTTATTGATGCTAACTGATGAACTCCGCTGATACAGGATATCTAATTATTTATTAGGAGTGCGAAGGCTGGGTTTTATGCTGCAAGATATAAGAGATTTGGAAACTTATGCTATGTGATTGGTGCTTTGCAAGGCTTTATGGGACACAAAAATAAAGACTTTAAAATTAAGGTCAGCATTTAAAAGAAACTCTTGGTATTTCCTAATGTGTTTTATACAATAACTGTAGTGCCTTAAGATGGTTTCAAAATGTAGAGGAGCATTTTGAGGGGGAGAGAGAGAGAGAGAGCATTTTTACCATTGTGATTGAATTTCATTTCTGTAGAATGCTATAACTTACAGGTTGACGACGGTGAATGGGAGCTGTATCCACAAGTAACTGCCATGTGCATTGGAAATGCTAAATACTTCGGCGGTGGTATGAAAATTGTGCCGAATGCTGATCCTTCCAATGGGAATCTAGAGGTAGAATTTACTTCCTTGGAAGTTTATTCCATTTCATAACTTCATTGTCTAATTAATTTGTGTGATGCCTTCGTTTGCATGGCAGGTGGTAATTCTTCAGGATTTTAAGTGGTACGACTTTATTCTGAATTTACATAAACTCTACAATGGGACACATTTGACAGTGAAAAACGTATCATCAAGAAGGTCTGTCATCTCTTTAAGCATTATGATGTGTTTAGATTTCTCATTTAGTGTCTCGATTTTCACTCTCCAACATCTGTATTATGCTGAAGACTTGTGTAAAGGTGAAGAAACAATAACTGAACTGGGATTCTATGGAGAATTTGTTATGTTCACTTCTGTCAAGTTTTGCTTGAGAAAAACTTACTTTCAAACTTATTGGAGATGTTTTATTCACCTAGCAGGGAAGCAATTAAGAGAAGTAATATTGGTCAAATTTCTGGCATTCTGATGTATCCTCTACAAAATTTAGAAAGCGTTTATGGTTATCATGCTTTTCTTGTTAAAATCTTTGTTTGCTTTGATGAACTTGGTTCGAAAAATTTTATCATCACTTGTCTATGGTTGAATAAATGACTGAGCATGATTTCAGATTTCAGCTCCCCCACTTTGAAATACAGTAGTAAGCAGTGGTGTTCTTTCCTTAGTAAAAACTTCTTGCTGCTGCTTCTGGACCTGTTTTCCTGCAACTATCATCAATTTATCATTTTGTTTTTCGAGCAGCGTCCGTTCTATCGAAGTGGAAGAAGTTTCGAGCAGTGGCAGCATTTATGTTCAATCTGATGGGGAACACTTGGGGTTTCTTCCAAGGAAATTCCATATTCTACCAGCTGCCATTGAAATGATCTGCTGA

mRNA sequence

ATGGCTCTATTTGTTAACACAAAAATCGCATTAGCATTGGTAGTTCATGTAAGATATTTTGCAGGGTTGAGGTGCCGAATCGATGTTGGAGTTGTCAATGAAGAAAATGGACAGGATCATTACTTCGCAAATGTTGCAGACATTCATTTGAGTGCGAAGGCTGGGTTTTATGCTGCAAGATATAAGAGATTTGGAAACTTATGCTATGTGATTGGTGCTTTGCAAGGCTTTATGGGACACAAAAATAAAGACTTTAAAATTAAGGTTGACGACGGTGAATGGGAGCTGTATCCACAAGTAACTGCCATGTGCATTGGAAATGCTAAATACTTCGGCGGTGGTATGAAAATTGTGCCGAATGCTGATCCTTCCAATGGGAATCTAGAGGTGGTAATTCTTCAGGATTTTAAGTGGTACGACTTTATTCTGAATTTACATAAACTCTACAATGGGACACATTTGACAGTGAAAAACGTATCATCAAGAAGCGTCCGTTCTATCGAAGTGGAAGAAGTTTCGAGCAGTGGCAGCATTTATGTTCAATCTGATGGGGAACACTTGGGGTTTCTTCCAAGGAAATTCCATATTCTACCAGCTGCCATTGAAATGATCTGCTGA

Coding sequence (CDS)

ATGGCTCTATTTGTTAACACAAAAATCGCATTAGCATTGGTAGTTCATGTAAGATATTTTGCAGGGTTGAGGTGCCGAATCGATGTTGGAGTTGTCAATGAAGAAAATGGACAGGATCATTACTTCGCAAATGTTGCAGACATTCATTTGAGTGCGAAGGCTGGGTTTTATGCTGCAAGATATAAGAGATTTGGAAACTTATGCTATGTGATTGGTGCTTTGCAAGGCTTTATGGGACACAAAAATAAAGACTTTAAAATTAAGGTTGACGACGGTGAATGGGAGCTGTATCCACAAGTAACTGCCATGTGCATTGGAAATGCTAAATACTTCGGCGGTGGTATGAAAATTGTGCCGAATGCTGATCCTTCCAATGGGAATCTAGAGGTGGTAATTCTTCAGGATTTTAAGTGGTACGACTTTATTCTGAATTTACATAAACTCTACAATGGGACACATTTGACAGTGAAAAACGTATCATCAAGAAGCGTCCGTTCTATCGAAGTGGAAGAAGTTTCGAGCAGTGGCAGCATTTATGTTCAATCTGATGGGGAACACTTGGGGTTTCTTCCAAGGAAATTCCATATTCTACCAGCTGCCATTGAAATGATCTGCTGA

Protein sequence

MALFVNTKIALALVVHVRYFAGLRCRIDVGVVNEENGQDHYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGFMGHKNKDFKIKVDDGEWELYPQVTAMCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFKWYDFILNLHKLYNGTHLTVKNVSSRSVRSIEVEEVSSSGSIYVQSDGEHLGFLPRKFHILPAAIEMIC
Homology
BLAST of Lcy01g007140 vs. ExPASy Swiss-Prot
Match: O82359 (Sphingoid long-chain bases kinase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=LCKB2 PE=2 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 3.8e-84
Identity = 140/187 (74.87%), Postives = 166/187 (88.77%), Query Frame = 0

Query: 18  RYFAGLRCRIDVGVVNEENGQDHYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGF 77
           R   G+R RIDVGV+++E    HYF NVAD+HLSAKAGFYA++YK+FGNLCYVIGALQ F
Sbjct: 177 RIARGMRSRIDVGVIDKEGKDLHYFINVADVHLSAKAGFYASKYKKFGNLCYVIGALQAF 236

Query: 78  MGHKNKDFKIKVDDGEWELYPQVTAMCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK 137
           MGH N+D +I+V+ GEWE+YPQVTA+C+GNAKYFGGGMKI PNA P NGNLEVV+LQ+FK
Sbjct: 237 MGHHNQDMRIRVNGGEWEIYPQVTALCVGNAKYFGGGMKITPNAIPGNGNLEVVVLQNFK 296

Query: 138 WYDFILNLHKLYNGTHLTVKNVSSRSVRSIEVEEVSSSGSIYVQSDGEHLGFLPRKFHIL 197
           WYDF+L LHKLYNGTHL+V NV+SRSV+SIEVEE++ SGSIYVQSDGEHLGFLPRKF +L
Sbjct: 297 WYDFVLKLHKLYNGTHLSVNNVTSRSVQSIEVEEITDSGSIYVQSDGEHLGFLPRKFQVL 356

Query: 198 PAAIEMI 205
           P AI++I
Sbjct: 357 PGAIDII 363

BLAST of Lcy01g007140 vs. ExPASy Swiss-Prot
Match: P9WP28 (Diacylglycerol kinase OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=dagK PE=1 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.7e-13
Identity = 55/200 (27.50%), Postives = 94/200 (47.00%), Query Frame = 0

Query: 6   NTKIALALVVHVRYFAGLRCRIDVGVVNEENGQDHYFANVADIHLSAKAGFYAARYK-RF 65
           N K A  +VV      G    ID+G + ++NG + +F  VA     +     A R +   
Sbjct: 115 NPKAAADIVVD-----GWTETIDLGRIQDDNGIEKWFGTVAATGFDSLVNDRANRMRWPH 174

Query: 66  GNLCYVIGALQGFMGHKNKDFKIKVDDGEWELYPQVTAMCIGNAKYFGGGMKIVPNADPS 125
           G + Y I  L      +   F++ V DG  E+   +T    GN + +GGG+ I PNAD S
Sbjct: 175 GRMRYYIAMLAELSRLRPLPFRL-VLDGTEEIVADLTLADFGNTRSYGGGLLICPNADHS 234

Query: 126 NGNLEVVILQDFKWYDFILNLHKLYNGTHLTVKNVSSRSVRSIEVEEVSSSGSIYVQSDG 185
           +G L++ + Q       +     ++ G H+ +  VS+   +++ VE       I V +DG
Sbjct: 235 DGLLDITMAQSDSRTKLLRLFPTIFKGAHVELDEVSTTRAKTVHVE----CPGINVYADG 294

Query: 186 EHLGFLPRKFHILPAAIEMI 205
           +    LP +   +PAA++++
Sbjct: 295 DFACPLPAEISAVPAALQVL 304

BLAST of Lcy01g007140 vs. ExPASy Swiss-Prot
Match: P9WP29 (Diacylglycerol kinase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=dagK PE=1 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.7e-13
Identity = 55/200 (27.50%), Postives = 94/200 (47.00%), Query Frame = 0

Query: 6   NTKIALALVVHVRYFAGLRCRIDVGVVNEENGQDHYFANVADIHLSAKAGFYAARYK-RF 65
           N K A  +VV      G    ID+G + ++NG + +F  VA     +     A R +   
Sbjct: 115 NPKAAADIVVD-----GWTETIDLGRIQDDNGIEKWFGTVAATGFDSLVNDRANRMRWPH 174

Query: 66  GNLCYVIGALQGFMGHKNKDFKIKVDDGEWELYPQVTAMCIGNAKYFGGGMKIVPNADPS 125
           G + Y I  L      +   F++ V DG  E+   +T    GN + +GGG+ I PNAD S
Sbjct: 175 GRMRYYIAMLAELSRLRPLPFRL-VLDGTEEIVADLTLADFGNTRSYGGGLLICPNADHS 234

Query: 126 NGNLEVVILQDFKWYDFILNLHKLYNGTHLTVKNVSSRSVRSIEVEEVSSSGSIYVQSDG 185
           +G L++ + Q       +     ++ G H+ +  VS+   +++ VE       I V +DG
Sbjct: 235 DGLLDITMAQSDSRTKLLRLFPTIFKGAHVELDEVSTTRAKTVHVE----CPGINVYADG 294

Query: 186 EHLGFLPRKFHILPAAIEMI 205
           +    LP +   +PAA++++
Sbjct: 295 DFACPLPAEISAVPAALQVL 304

BLAST of Lcy01g007140 vs. ExPASy TrEMBL
Match: A0A0A0KD28 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G006770 PE=4 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 1.6e-104
Identity = 185/205 (90.24%), Postives = 197/205 (96.10%), Query Frame = 0

Query: 1   MALFVNTKIALALVVHVRYFAGLRCRIDVGVVNEENGQDHYFANVADIHLSAKAGFYAAR 60
           MALFV+T IAL LVVH RY AGLR RIDVGVVNE+NG++HYFANVADIHLSAKAGFYAAR
Sbjct: 1   MALFVHTNIALPLVVHARYCAGLRTRIDVGVVNEDNGENHYFANVADIHLSAKAGFYAAR 60

Query: 61  YKRFGNLCYVIGALQGFMGHKNKDFKIKVDDGEWELYPQVTAMCIGNAKYFGGGMKIVPN 120
           YK+FGNLCYVIGALQGFMGH+N+DF+IKVDDGEWELYPQVTA+CIGNAKYFGGGMKIVPN
Sbjct: 61  YKKFGNLCYVIGALQGFMGHQNRDFRIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPN 120

Query: 121 ADPSNGNLEVVILQDFKWYDFILNLHKLYNGTHLTVKNVSSRSVRSIEVEEVSSSGSIYV 180
            DPSN +LEVVILQDFKWYDFILNLHK+YNGTHLTVKNV+SRSVRSIEVEEVS SGSIYV
Sbjct: 121 VDPSNRSLEVVILQDFKWYDFILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVSCSGSIYV 180

Query: 181 QSDGEHLGFLPRKFHILPAAIEMIC 206
           QSDGEHLGFLPRKFHILPAAIEMIC
Sbjct: 181 QSDGEHLGFLPRKFHILPAAIEMIC 205

BLAST of Lcy01g007140 vs. ExPASy TrEMBL
Match: A0A6J1HNB5 (sphingoid long-chain bases kinase 2, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111464490 PE=4 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 9.8e-99
Identity = 174/188 (92.55%), Postives = 183/188 (97.34%), Query Frame = 0

Query: 18  RYFAGLRCRIDVGVVNEENGQDHYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGF 77
           R   GLR RID+GVVN+ENG+ HYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGF
Sbjct: 177 RIAKGLRSRIDIGVVNKENGEYHYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGF 236

Query: 78  MGHKNKDFKIKVDDGEWELYPQVTAMCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK 137
           +GHKNKDFKIKVD+GEWELYPQVT++CIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK
Sbjct: 237 IGHKNKDFKIKVDNGEWELYPQVTSLCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK 296

Query: 138 WYDFILNLHKLYNGTHLTVKNVSSRSVRSIEVEEVSSSGSIYVQSDGEHLGFLPRKFHIL 197
           WYDFILNLHK+YNGTHLTVKNVSSRSVRSIEVEEVSSSGSIYVQ+DGEHLGFLPRKFHIL
Sbjct: 297 WYDFILNLHKIYNGTHLTVKNVSSRSVRSIEVEEVSSSGSIYVQADGEHLGFLPRKFHIL 356

Query: 198 PAAIEMIC 206
           PAAIEMIC
Sbjct: 357 PAAIEMIC 364

BLAST of Lcy01g007140 vs. ExPASy TrEMBL
Match: A0A5D3BVA2 (Sphingoid long-chain bases kinase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G001350 PE=4 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 4.9e-98
Identity = 171/188 (90.96%), Postives = 183/188 (97.34%), Query Frame = 0

Query: 18  RYFAGLRCRIDVGVVNEENGQDHYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGF 77
           R   GLR RIDVGVVNE+NG++HYFANVADIHLSAKAGFYAARYK+FGNLCYVIGALQGF
Sbjct: 179 RIAKGLRTRIDVGVVNEDNGENHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGF 238

Query: 78  MGHKNKDFKIKVDDGEWELYPQVTAMCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK 137
           MGH+N+DFKIKVDDGEWELYPQVTA+CIGNAKYFGGGMKIVPNADPSNG+LEVVILQDFK
Sbjct: 239 MGHQNRDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVILQDFK 298

Query: 138 WYDFILNLHKLYNGTHLTVKNVSSRSVRSIEVEEVSSSGSIYVQSDGEHLGFLPRKFHIL 197
           WYDFILNLHK+YNGTHLTVKNV+S+S+RSIEVEEVS SGSIYVQSDGEHLGFLPRKFHIL
Sbjct: 299 WYDFILNLHKIYNGTHLTVKNVTSKSIRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHIL 358

Query: 198 PAAIEMIC 206
           PAAIEMIC
Sbjct: 359 PAAIEMIC 366

BLAST of Lcy01g007140 vs. ExPASy TrEMBL
Match: A0A1S3C7U6 (sphingoid long-chain bases kinase 2, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103497689 PE=4 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 4.9e-98
Identity = 171/188 (90.96%), Postives = 183/188 (97.34%), Query Frame = 0

Query: 18  RYFAGLRCRIDVGVVNEENGQDHYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGF 77
           R   GLR RIDVGVVNE+NG++HYFANVADIHLSAKAGFYAARYK+FGNLCYVIGALQGF
Sbjct: 179 RIAKGLRTRIDVGVVNEDNGENHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGF 238

Query: 78  MGHKNKDFKIKVDDGEWELYPQVTAMCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK 137
           MGH+N+DFKIKVDDGEWELYPQVTA+CIGNAKYFGGGMKIVPNADPSNG+LEVVILQDFK
Sbjct: 239 MGHQNRDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVILQDFK 298

Query: 138 WYDFILNLHKLYNGTHLTVKNVSSRSVRSIEVEEVSSSGSIYVQSDGEHLGFLPRKFHIL 197
           WYDFILNLHK+YNGTHLTVKNV+S+S+RSIEVEEVS SGSIYVQSDGEHLGFLPRKFHIL
Sbjct: 299 WYDFILNLHKIYNGTHLTVKNVTSKSIRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHIL 358

Query: 198 PAAIEMIC 206
           PAAIEMIC
Sbjct: 359 PAAIEMIC 366

BLAST of Lcy01g007140 vs. ExPASy TrEMBL
Match: A0A6J1KFE4 (sphingoid long-chain bases kinase 2, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111494546 PE=4 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 1.1e-97
Identity = 172/188 (91.49%), Postives = 182/188 (96.81%), Query Frame = 0

Query: 18  RYFAGLRCRIDVGVVNEENGQDHYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGF 77
           R   GLR RID+GVVN+ENG+ HYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGF
Sbjct: 177 RIAKGLRSRIDIGVVNKENGEYHYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGF 236

Query: 78  MGHKNKDFKIKVDDGEWELYPQVTAMCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK 137
           +GHKNKD KIKVD+GEWELYPQVT++CIGNAKYFGGGMKIVPNADPSNGNLE+VILQDFK
Sbjct: 237 IGHKNKDLKIKVDNGEWELYPQVTSLCIGNAKYFGGGMKIVPNADPSNGNLELVILQDFK 296

Query: 138 WYDFILNLHKLYNGTHLTVKNVSSRSVRSIEVEEVSSSGSIYVQSDGEHLGFLPRKFHIL 197
           WYDFILNLHK+YNGTHLTVKNVSSRSVRSIEVEEVSSSGSIYVQ+DGEHLGFLPRKFHIL
Sbjct: 297 WYDFILNLHKIYNGTHLTVKNVSSRSVRSIEVEEVSSSGSIYVQADGEHLGFLPRKFHIL 356

Query: 198 PAAIEMIC 206
           PAAIEMIC
Sbjct: 357 PAAIEMIC 364

BLAST of Lcy01g007140 vs. NCBI nr
Match: KAG7025983.1 (Sphingoid long-chain bases kinase 2, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 369.4 bits (947), Expect = 2.0e-98
Identity = 174/188 (92.55%), Postives = 183/188 (97.34%), Query Frame = 0

Query: 18  RYFAGLRCRIDVGVVNEENGQDHYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGF 77
           R   GLR RID+GVVN+ENG+ HYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGF
Sbjct: 177 RIAKGLRSRIDIGVVNKENGEYHYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGF 236

Query: 78  MGHKNKDFKIKVDDGEWELYPQVTAMCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK 137
           +GHKNKDFKIKVD+GEWELYPQVT++CIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK
Sbjct: 237 IGHKNKDFKIKVDNGEWELYPQVTSLCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK 296

Query: 138 WYDFILNLHKLYNGTHLTVKNVSSRSVRSIEVEEVSSSGSIYVQSDGEHLGFLPRKFHIL 197
           WYDFILNLHK+YNGTHLTVKNVSSRSVRSIEVEEVSSSGSIYVQ+DGEHLGFLPRKFHIL
Sbjct: 297 WYDFILNLHKIYNGTHLTVKNVSSRSVRSIEVEEVSSSGSIYVQADGEHLGFLPRKFHIL 356

Query: 198 PAAIEMIC 206
           PAAIEMIC
Sbjct: 357 PAAIEMIC 364

BLAST of Lcy01g007140 vs. NCBI nr
Match: XP_023514489.1 (sphingoid long-chain bases kinase 2, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 369.4 bits (947), Expect = 2.0e-98
Identity = 174/188 (92.55%), Postives = 183/188 (97.34%), Query Frame = 0

Query: 18  RYFAGLRCRIDVGVVNEENGQDHYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGF 77
           R   GLR RID+GVVN+ENG+ HYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGF
Sbjct: 177 RIAKGLRSRIDIGVVNKENGEYHYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGF 236

Query: 78  MGHKNKDFKIKVDDGEWELYPQVTAMCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK 137
           +GHKNKDFKIKVD+GEWELYPQVT++CIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK
Sbjct: 237 IGHKNKDFKIKVDNGEWELYPQVTSLCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK 296

Query: 138 WYDFILNLHKLYNGTHLTVKNVSSRSVRSIEVEEVSSSGSIYVQSDGEHLGFLPRKFHIL 197
           WYDFILNLHK+YNGTHLTVKNVSSRSVRSIEVEEVSSSGSIYVQ+DGEHLGFLPRKFHIL
Sbjct: 297 WYDFILNLHKIYNGTHLTVKNVSSRSVRSIEVEEVSSSGSIYVQADGEHLGFLPRKFHIL 356

Query: 198 PAAIEMIC 206
           PAAIEMIC
Sbjct: 357 PAAIEMIC 364

BLAST of Lcy01g007140 vs. NCBI nr
Match: KAG6593652.1 (Sphingoid long-chain bases kinase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 369.4 bits (947), Expect = 2.0e-98
Identity = 174/188 (92.55%), Postives = 183/188 (97.34%), Query Frame = 0

Query: 18  RYFAGLRCRIDVGVVNEENGQDHYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGF 77
           R   GLR RID+GVVN+ENG+ HYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGF
Sbjct: 243 RIAKGLRSRIDIGVVNKENGEYHYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGF 302

Query: 78  MGHKNKDFKIKVDDGEWELYPQVTAMCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK 137
           +GHKNKDFKIKVD+GEWELYPQVT++CIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK
Sbjct: 303 IGHKNKDFKIKVDNGEWELYPQVTSLCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK 362

Query: 138 WYDFILNLHKLYNGTHLTVKNVSSRSVRSIEVEEVSSSGSIYVQSDGEHLGFLPRKFHIL 197
           WYDFILNLHK+YNGTHLTVKNVSSRSVRSIEVEEVSSSGSIYVQ+DGEHLGFLPRKFHIL
Sbjct: 363 WYDFILNLHKIYNGTHLTVKNVSSRSVRSIEVEEVSSSGSIYVQADGEHLGFLPRKFHIL 422

Query: 198 PAAIEMIC 206
           PAAIEMIC
Sbjct: 423 PAAIEMIC 430

BLAST of Lcy01g007140 vs. NCBI nr
Match: XP_022964479.1 (sphingoid long-chain bases kinase 2, mitochondrial [Cucurbita moschata])

HSP 1 Score: 369.4 bits (947), Expect = 2.0e-98
Identity = 174/188 (92.55%), Postives = 183/188 (97.34%), Query Frame = 0

Query: 18  RYFAGLRCRIDVGVVNEENGQDHYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGF 77
           R   GLR RID+GVVN+ENG+ HYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGF
Sbjct: 177 RIAKGLRSRIDIGVVNKENGEYHYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGF 236

Query: 78  MGHKNKDFKIKVDDGEWELYPQVTAMCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK 137
           +GHKNKDFKIKVD+GEWELYPQVT++CIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK
Sbjct: 237 IGHKNKDFKIKVDNGEWELYPQVTSLCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK 296

Query: 138 WYDFILNLHKLYNGTHLTVKNVSSRSVRSIEVEEVSSSGSIYVQSDGEHLGFLPRKFHIL 197
           WYDFILNLHK+YNGTHLTVKNVSSRSVRSIEVEEVSSSGSIYVQ+DGEHLGFLPRKFHIL
Sbjct: 297 WYDFILNLHKIYNGTHLTVKNVSSRSVRSIEVEEVSSSGSIYVQADGEHLGFLPRKFHIL 356

Query: 198 PAAIEMIC 206
           PAAIEMIC
Sbjct: 357 PAAIEMIC 364

BLAST of Lcy01g007140 vs. NCBI nr
Match: XP_038876180.1 (sphingoid long-chain bases kinase 2, mitochondrial [Benincasa hispida])

HSP 1 Score: 367.5 bits (942), Expect = 7.7e-98
Identity = 174/188 (92.55%), Postives = 181/188 (96.28%), Query Frame = 0

Query: 18  RYFAGLRCRIDVGVVNEENGQDHYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGF 77
           R   GLR RIDVGVVNEENG  HYFANVADIHLSAKAGFYA+RYK+FGNLCYVIGALQGF
Sbjct: 178 RIAKGLRSRIDVGVVNEENGGYHYFANVADIHLSAKAGFYASRYKKFGNLCYVIGALQGF 237

Query: 78  MGHKNKDFKIKVDDGEWELYPQVTAMCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK 137
           MGH+N+DFKIKVDDGEWELYPQVTA+CIGNAKYFGGGMKIVPNADP NGNLEVVILQDFK
Sbjct: 238 MGHQNRDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPCNGNLEVVILQDFK 297

Query: 138 WYDFILNLHKLYNGTHLTVKNVSSRSVRSIEVEEVSSSGSIYVQSDGEHLGFLPRKFHIL 197
           WYDFILNLHK+YNGTHLTVKNV+SRSVRSIEVEEVSSSGSIYVQSDGEHLGFLPRKFHIL
Sbjct: 298 WYDFILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVSSSGSIYVQSDGEHLGFLPRKFHIL 357

Query: 198 PAAIEMIC 206
           PAAIEMIC
Sbjct: 358 PAAIEMIC 365

BLAST of Lcy01g007140 vs. TAIR 10
Match: AT2G46090.1 (Diacylglycerol kinase family protein )

HSP 1 Score: 312.4 bits (799), Expect = 2.7e-85
Identity = 140/187 (74.87%), Postives = 166/187 (88.77%), Query Frame = 0

Query: 18  RYFAGLRCRIDVGVVNEENGQDHYFANVADIHLSAKAGFYAARYKRFGNLCYVIGALQGF 77
           R   G+R RIDVGV+++E    HYF NVAD+HLSAKAGFYA++YK+FGNLCYVIGALQ F
Sbjct: 177 RIARGMRSRIDVGVIDKEGKDLHYFINVADVHLSAKAGFYASKYKKFGNLCYVIGALQAF 236

Query: 78  MGHKNKDFKIKVDDGEWELYPQVTAMCIGNAKYFGGGMKIVPNADPSNGNLEVVILQDFK 137
           MGH N+D +I+V+ GEWE+YPQVTA+C+GNAKYFGGGMKI PNA P NGNLEVV+LQ+FK
Sbjct: 237 MGHHNQDMRIRVNGGEWEIYPQVTALCVGNAKYFGGGMKITPNAIPGNGNLEVVVLQNFK 296

Query: 138 WYDFILNLHKLYNGTHLTVKNVSSRSVRSIEVEEVSSSGSIYVQSDGEHLGFLPRKFHIL 197
           WYDF+L LHKLYNGTHL+V NV+SRSV+SIEVEE++ SGSIYVQSDGEHLGFLPRKF +L
Sbjct: 297 WYDFVLKLHKLYNGTHLSVNNVTSRSVQSIEVEEITDSGSIYVQSDGEHLGFLPRKFQVL 356

Query: 198 PAAIEMI 205
           P AI++I
Sbjct: 357 PGAIDII 363

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O823593.8e-8474.87Sphingoid long-chain bases kinase 2, mitochondrial OS=Arabidopsis thaliana OX=37... [more]
P9WP282.7e-1327.50Diacylglycerol kinase OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) ... [more]
P9WP292.7e-1327.50Diacylglycerol kinase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) ... [more]
Match NameE-valueIdentityDescription
A0A0A0KD281.6e-10490.24Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G006770 PE=4 SV=1[more]
A0A6J1HNB59.8e-9992.55sphingoid long-chain bases kinase 2, mitochondrial OS=Cucurbita moschata OX=3662... [more]
A0A5D3BVA24.9e-9890.96Sphingoid long-chain bases kinase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A1S3C7U64.9e-9890.96sphingoid long-chain bases kinase 2, mitochondrial OS=Cucumis melo OX=3656 GN=LO... [more]
A0A6J1KFE41.1e-9791.49sphingoid long-chain bases kinase 2, mitochondrial OS=Cucurbita maxima OX=3661 G... [more]
Match NameE-valueIdentityDescription
KAG7025983.12.0e-9892.55Sphingoid long-chain bases kinase 2, mitochondrial [Cucurbita argyrosperma subsp... [more]
XP_023514489.12.0e-9892.55sphingoid long-chain bases kinase 2, mitochondrial [Cucurbita pepo subsp. pepo][more]
KAG6593652.12.0e-9892.55Sphingoid long-chain bases kinase 2, mitochondrial, partial [Cucurbita argyrospe... [more]
XP_022964479.12.0e-9892.55sphingoid long-chain bases kinase 2, mitochondrial [Cucurbita moschata][more]
XP_038876180.17.7e-9892.55sphingoid long-chain bases kinase 2, mitochondrial [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT2G46090.12.7e-8574.87Diacylglycerol kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D2.60.200.40coord: 28..198
e-value: 1.5E-33
score: 117.9
NoneNo IPR availablePANTHERPTHR12358:SF94BNAA04G26670D PROTEINcoord: 19..197
NoneNo IPR availablePANTHERPTHR12358SPHINGOSINE KINASEcoord: 19..197
IPR016064NAD kinase/diacylglycerol kinase-like domain superfamilySUPERFAMILY111331NAD kinase/diacylglycerol kinase-likecoord: 19..205

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy01g007140.1Lcy01g007140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
biological_process GO:0006671 phytosphingosine metabolic process
biological_process GO:0009409 response to cold
cellular_component GO:0005739 mitochondrion
molecular_function GO:0003951 NAD+ kinase activity