Lcy01g006560 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy01g006560
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	gibberellin 20-oxidase-like protein
Location	Chr01: 10152565 .. 10153925 (-)
RNA-Seq Expression	Lcy01g006560
Synteny	Lcy01g006560

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTCAAGTCAAACCCTTTCACCAATTACAATTATGAATCTGTTAGCAATTTTTCAAATCACTGTGTTCTTGTTTTTATTATCATAATCATGGTTGCTTTTCCAAGTGACAGGAATCAACCCACTAATGATTAAAACTAAAGACAATAAAAAAGAAGTTATGACCATTTGCATCTTTCCTTTTAAGCAATATAATTATCCACCTATGGAGTATAGGCTAGCTAGCTATAGACAAATTAGTAGGCAAACATGTCTGTATCTAAGGCCTCAGTGGAACTCCCCATTTTAGACATTTCTCAGCCATTATCTCCATCTTCCCTTTCTTCACTAGCTGATGCCTGCAGAAAATGGGGTTTTTTCCATATCAAAAACCATGGGATCTCCAAAGATATCTACAACAAACTCCATTCCTTCTCCAATGAAATCTTCAGCCTCCCTTCTGAAACCAAGCTCAAAATTGGCCCTCACTCTTCTTTGAACACTTACACTCCCCATTTCATAGCCTCCCCTTTCTTTGAAAGCCTCAGAGTCTCTGGTCCAAACTTCTCAGCCTCTGCTCAGAGTTCAGCAGATTTCCTCTTTAACCAAAAGAGCCCTCACCAGTTCAGGTACACATAGTTCACAAACACATCCACTTCAAAAACTTTCTATCCCTTTAATCATTACTTCTATTGTTCTAACAATCTACTTTCATGTGCATTTAAGTGAGATATTGCAAGAATATGGAGACAAAATGACAGAGCTATCAAGGAAGATTATAAAGAACATACTGATGAGCTTAGGGGAAGGTTTTGAGAAGACATACCATGAACCCCATTTCAAGAATTGCCATGGATATCTCAGAATAAACAACTACACCGCTCCAGAAGCCTTGCAAGACGATGAAGAACCAGAAGGACTTGGAATGCACACGGATATGAGCTGCGTGACCATCGTGTACCAAGATCAAACAGGTGGTCTGCAAGTGAAATCGAGCGAAGGGAAATGGGTCGACATAAGCCCATTTGAGGAATCCGAATCCGAAAGAGAATCTGAATCCGAAGCTCTTGTGGTGAACATCGGCGACCTGCTTCAAGCTTGGAGCAACGACAGGCTGAGATCGTCGGAGCACAGAGTGGTTTTGAGGCGACCGGCGATCAATCGGTTCTCGATTGCGTTCTTTTGGGGGTTTGAGGACGAGAAGGCGGTATCGGCGCCGGAGGAGGTGGTCGGCGACGGCGGTGAGAGGCTGTATAAGCCGTTTGTTTGCGCGGATTATTTGAGGTTCAGAGAGAATGATGAAAGAGGGAAATTTGAAAAGGTTGGTTTCACTGTGAGAGACTTTGCTGGGATTAACAAGGGTTTTGAAGAAATATGAATTGCTA

mRNA sequence

GTCAAGTCAAACCCTTTCACCAATTACAATTATGAATCTGTTAGCAATTTTTCAAATCACTGTGTTCTTGTTTTTATTATCATAATCATGGTTGCTTTTCCAAGTGACAGGAATCAACCCACTAATGATTAAAACTAAAGACAATAAAAAAGAAGTTATGACCATTTGCATCTTTCCTTTTAAGCAATATAATTATCCACCTATGGAGTATAGGCTAGCTAGCTATAGACAAATTAGTAGGCAAACATGTCTGTATCTAAGGCCTCAGTGGAACTCCCCATTTTAGACATTTCTCAGCCATTATCTCCATCTTCCCTTTCTTCACTAGCTGATGCCTGCAGAAAATGGGGTTTTTTCCATATCAAAAACCATGGGATCTCCAAAGATATCTACAACAAACTCCATTCCTTCTCCAATGAAATCTTCAGCCTCCCTTCTGAAACCAAGCTCAAAATTGGCCCTCACTCTTCTTTGAACACTTACACTCCCCATTTCATAGCCTCCCCTTTCTTTGAAAGCCTCAGAGTCTCTGGTCCAAACTTCTCAGCCTCTGCTCAGAGTTCAGCAGATTTCCTCTTTAACCAAAAGAGCCCTCACCAGTTCAGTGAGATATTGCAAGAATATGGAGACAAAATGACAGAGCTATCAAGGAAGATTATAAAGAACATACTGATGAGCTTAGGGGAAGGTTTTGAGAAGACATACCATGAACCCCATTTCAAGAATTGCCATGGATATCTCAGAATAAACAACTACACCGCTCCAGAAGCCTTGCAAGACGATGAAGAACCAGAAGGACTTGGAATGCACACGGATATGAGCTGCGTGACCATCGTGTACCAAGATCAAACAGGTGGTCTGCAAGTGAAATCGAGCGAAGGGAAATGGGTCGACATAAGCCCATTTGAGGAATCCGAATCCGAAAGAGAATCTGAATCCGAAGCTCTTGTGGTGAACATCGGCGACCTGCTTCAAGCTTGGAGCAACGACAGGCTGAGATCGTCGGAGCACAGAGTGGTTTTGAGGCGACCGGCGATCAATCGGTTCTCGATTGCGTTCTTTTGGGGGTTTGAGGACGAGAAGGCGGTATCGGCGCCGGAGGAGGTGGTCGGCGACGGCGGTGAGAGGCTGTATAAGCCGTTTGTTTGCGCGGATTATTTGAGGTTCAGAGAGAATGATGAAAGAGGGAAATTTGAAAAGGTTGGTTTCACTGTGAGAGACTTTGCTGGGATTAACAAGGGTTTTGAAGAAATATGAATTGCTA

Coding sequence (CDS)

ATGTCTGTATCTAAGGCCTCAGTGGAACTCCCCATTTTAGACATTTCTCAGCCATTATCTCCATCTTCCCTTTCTTCACTAGCTGATGCCTGCAGAAAATGGGGTTTTTTCCATATCAAAAACCATGGGATCTCCAAAGATATCTACAACAAACTCCATTCCTTCTCCAATGAAATCTTCAGCCTCCCTTCTGAAACCAAGCTCAAAATTGGCCCTCACTCTTCTTTGAACACTTACACTCCCCATTTCATAGCCTCCCCTTTCTTTGAAAGCCTCAGAGTCTCTGGTCCAAACTTCTCAGCCTCTGCTCAGAGTTCAGCAGATTTCCTCTTTAACCAAAAGAGCCCTCACCAGTTCAGTGAGATATTGCAAGAATATGGAGACAAAATGACAGAGCTATCAAGGAAGATTATAAAGAACATACTGATGAGCTTAGGGGAAGGTTTTGAGAAGACATACCATGAACCCCATTTCAAGAATTGCCATGGATATCTCAGAATAAACAACTACACCGCTCCAGAAGCCTTGCAAGACGATGAAGAACCAGAAGGACTTGGAATGCACACGGATATGAGCTGCGTGACCATCGTGTACCAAGATCAAACAGGTGGTCTGCAAGTGAAATCGAGCGAAGGGAAATGGGTCGACATAAGCCCATTTGAGGAATCCGAATCCGAAAGAGAATCTGAATCCGAAGCTCTTGTGGTGAACATCGGCGACCTGCTTCAAGCTTGGAGCAACGACAGGCTGAGATCGTCGGAGCACAGAGTGGTTTTGAGGCGACCGGCGATCAATCGGTTCTCGATTGCGTTCTTTTGGGGGTTTGAGGACGAGAAGGCGGTATCGGCGCCGGAGGAGGTGGTCGGCGACGGCGGTGAGAGGCTGTATAAGCCGTTTGTTTGCGCGGATTATTTGAGGTTCAGAGAGAATGATGAAAGAGGGAAATTTGAAAAGGTTGGTTTCACTGTGAGAGACTTTGCTGGGATTAACAAGGGTTTTGAAGAAATATGA

Protein sequence

MSVSKASVELPILDISQPLSPSSLSSLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIFSLPSETKLKIGPHSSLNTYTPHFIASPFFESLRVSGPNFSASAQSSADFLFNQKSPHQFSEILQEYGDKMTELSRKIIKNILMSLGEGFEKTYHEPHFKNCHGYLRINNYTAPEALQDDEEPEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDISPFEESESERESESEALVVNIGDLLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKAVSAPEEVVGDGGERLYKPFVCADYLRFRENDERGKFEKVGFTVRDFAGINKGFEEI

Homology

[Close]

BLAST of Lcy01g006560 vs. ExPASy Swiss-Prot
Match: F4KBY0 (Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310 PE=2 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 3.7e-100
Identity = 188/334 (56.29%), Postives = 238/334 (71.26%), Query Frame = 0


Query: 7   SVELPILDISQPLSPSSLSSLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIFSLPSET 66
           S+ELP+ DIS+PLS SSL+SL DAC++WGFF++ NHG+S+D+Y KL  FS  +F L  E 
Sbjct: 2   SLELPVFDISKPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFSTGVFELEDEE 61

Query: 67  KLKIGPHSSLNTYTPHFIASPFFESLRVSGPNFSASAQSSADFLFNQKSPHQFSEILQEY 126
           K+K+G     + YTP FIASPFFESLRVSGP+F ASA+SS D   +Q +  +FS +++EY
Sbjct: 62  KMKMG----ASNYTPRFIASPFFESLRVSGPDFYASAKSSVDAFSDQATDEEFSGLMKEY 121

Query: 127 GDKMTELSRKIIKNILMSLGEGFEKTYHEPHFKNCHGYLRINNYTAPEALQDDEEP---- 186
           G+KMT+L  KI+K IL S G+     Y+E  F NCHGY RINNYT P   +DD       
Sbjct: 122 GEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNGDEQ 181

Query: 187 ---EGLGMHTDMSCVTIVYQDQTGGLQVKSSEG-KWVDISPFEESESERESESEALVVNI 246
              EGLGMHTDMSC+TIV QD  GGLQV++ +G   +DI+P          + EALVVN+
Sbjct: 182 DLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINP----------KDEALVVNV 241

Query: 247 GDLLQAWSNDRLRSSEHRVVLRRPAI--NRFSIAFFWGFEDEKAVSAPEEVVGD-GGERL 306
           GDLL AW+N RLRSS+HRV+L+R     NRFS+AFFW F+D K V AP+EVVG   G R+
Sbjct: 242 GDLLHAWTNGRLRSSQHRVILKRRGFVGNRFSLAFFWCFDDGKVVFAPDEVVGGCEGMRV 301

Query: 307 YKPFVCADYLRFRENDERGKFEKVGFTVRDFAGI 330
           ++ F C DYLRFRE++E+GKFEKVG TV DFA I
Sbjct: 302 FRSFKCGDYLRFRESNEKGKFEKVGDTVEDFARI 321

[Close]

BLAST of Lcy01g006560 vs. ExPASy Swiss-Prot
Match: Q9C971 (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 6.8e-30
Identity = 98/317 (30.91%), Postives = 150/317 (47.32%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSSLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           +S       +P++D+S   +P   + + DA + WG F I NHGIS+ + + + S S  +F
Sbjct: 40  VSFDAVEESIPVIDLS---NPDVTTLIGDASKTWGAFQIANHGISQKLLDDIESLSKTLF 99

Query: 61  SLPSETKLKIGPHSSLNTYTPHFIASPFFESLRVSGPNFSASAQSSADFLFNQKSPH--- 120
            +PSE KL+        +       SPFFE  ++    F+ +  S  +  FN   PH   
Sbjct: 100 DMPSERKLEAASSDKGVSGYGEPRISPFFEK-KMWSEGFTIADDSYRNH-FNTLWPHDHT 159

Query: 121 QFSEILQEYGDKMTELSRKIIKNILMSLGEGFE-----KTYHEPHFKNCHGYLRINNYTA 180
           ++  I+QEY D+M +L+ +++  IL SLG   E         +   K   G +R+N+Y  
Sbjct: 160 KYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIEWAHKLEKSGSKVGRGAIRLNHY-- 219

Query: 181 PEALQDDEEPEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDISPFEESESERESESE 240
                + E   GL  HTD + +TI++Q  TGGLQV   E  WV + P             
Sbjct: 220 -PVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWVTVEP----------APG 279

Query: 241 ALVVNIGDLLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKAVSAP-EEVVGDG 300
            LVVNIGDL    SN ++ S  HR  +     +R SIA+ WG        AP  ++ G  
Sbjct: 280 VLVVNIGDLFHILSNGKIPSVVHRAKVNHTR-SRISIAYLWGGPAGDVQIAPISKLTGPA 337

Query: 301 GERLYKPFVCADYLRFR 309
              LY+     +YL+ +
Sbjct: 340 EPSLYRSITWKEYLQIK 337

[Close]

BLAST of Lcy01g006560 vs. ExPASy Swiss-Prot
Match: Q9LHN8 (Feruloyl CoA ortho-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=F6'H1 PE=1 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 5.8e-29
Identity = 91/315 (28.89%), Postives = 154/315 (48.89%), Query Frame = 0


Query: 3   VSKASVELPILDISQPLSPSSLSSLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIFSL 62
           V++    +P++D+S P       ++ DA  KWGFF + NHG+  ++ + + + +++ F+L
Sbjct: 55  VNETDEAIPVIDMSNPDEDRVAEAVCDAAEKWGFFQVINHGVPLEVLDDVKAATHKFFNL 114

Query: 63  PSETKLKIGPHSSLNTYTPHFIASPFFESLRVSGPNFSASAQSSAD-------FLFNQKS 122
           P E K K    +SL+T T  F            G +FS  A+ + +       F  ++  
Sbjct: 115 PVEEKRKFTKENSLST-TVRF------------GTSFSPLAEQALEWKDYLSLFFVSEAE 174

Query: 123 PHQF-SEILQEYGDKMTELSRKIIKNILMSLGEGFE-KTYHEPHFKNCHGYLRINNYTAP 182
             QF  +I +    +    S+K+++ +L  LG+    K   E       G +R+N    P
Sbjct: 175 AEQFWPDICRNETLEYINKSKKMVRRLLEYLGKNLNVKELDETKESLFMGSIRVNLNYYP 234

Query: 183 EALQDDEEPEGLGMHTDMSCVTIVYQDQTGGLQVKS-SEGKWVDISPFEESESERESESE 242
               + +   G+G H+D+S +TI+ QDQ GGL V+S + G WV + P   S         
Sbjct: 235 -ICPNPDLTVGVGRHSDVSSLTILLQDQIGGLHVRSLASGNWVHVPPVAGS--------- 294

Query: 243 ALVVNIGDLLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKAVSAPEEVVGDGG 302
             V+NIGD +Q  SN   +S EHR VL     NR S+  F   + E  +    EV+ +G 
Sbjct: 295 -FVINIGDAMQIMSNGLYKSVEHR-VLANGYNNRISVPIFVNPKPESVIGPLPEVIANGE 344

Query: 303 ERLYKPFVCADYLRF 308
           E +Y+  + +DY+++
Sbjct: 355 EPIYRDVLYSDYVKY 344

[Close]

BLAST of Lcy01g006560 vs. ExPASy Swiss-Prot
Match: Q9C899 (Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=F6'H2 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 2.2e-28
Identity = 96/306 (31.37%), Postives = 148/306 (48.37%), Query Frame = 0


Query: 10  LPILDISQPLSPSSLSSLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIFSLPSETKLK 69
           +P++DIS     S   ++ DA  +WGFF + NHG+S ++   + + ++  F LP E K K
Sbjct: 62  IPVIDISNLDEKSVSKAVCDAAEEWGFFQVINHGVSMEVLENMKTATHRFFGLPVEEKRK 121

Query: 70  IGPHSSLNT-------YTPHFIASPFFESLRVSGPNFSASAQSSADFLFNQKSPHQFSEI 129
                SL+T       ++PH  A    E        F + A++S      Q  P      
Sbjct: 122 FSREKSLSTNVRFGTSFSPH--AEKALEWKDYLSLFFVSEAEAS------QLWPDSCRSE 181

Query: 130 LQEYGDKMTELSRKIIKNILMSLGEGFE-KTYHEPHFKNCHGYLRINNYTAPEALQDDEE 189
             EY   M E ++ ++K +L  LGE    K   +       G  RIN    P    + E 
Sbjct: 182 TLEY---MNE-TKPLVKKLLRFLGENLNVKELDKTKESFFMGSTRINLNYYP-ICPNPEL 241

Query: 190 PEGLGMHTDMSCVTIVYQDQTGGLQVKS-SEGKWVDISPFEESESERESESEALVVNIGD 249
             G+G H+D+S +TI+ QD+ GGL V+S + G+WV + P           S +LV+NIGD
Sbjct: 242 TVGVGRHSDVSSLTILLQDEIGGLHVRSLTTGRWVHVPPI----------SGSLVINIGD 301

Query: 250 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKAVSAPEEVVGDGGERLYKPFV 307
            +Q  SN R +S EHR VL   + NR S+  F   + E  +    EV+ +G + +YK  +
Sbjct: 302 AMQIMSNGRYKSVEHR-VLANGSYNRISVPIFVSPKPESVIGPLLEVIENGEKPVYKDIL 343

[Close]

BLAST of Lcy01g006560 vs. ExPASy Swiss-Prot
Match: Q39103 (Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 SV=2)

HSP 1 Score: 122.1 bits (305), Expect = 1.2e-26
Identity = 88/302 (29.14%), Postives = 140/302 (46.36%), Query Frame = 0


Query: 10  LPILDISQPLSPSSLSSLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIFSLPSETKLK 69
           +P++D+     P + + +  ACR WG F I NHG+   +   +   +  +F LP + KLK
Sbjct: 57  IPLIDLDH---PDATNQIGHACRTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLK 116

Query: 70  IG-PHSSLNTYTPHFIASPFFESLRVSGPNFSASAQSSADFLFNQKSPH-QFSEILQEYG 129
                + ++ Y    IAS F + +   G  F+ +     DF       H  + +I++EY 
Sbjct: 117 SARSETGVSGYGVARIASFFNKQMWSEG--FTITGSPLNDFRKLWPQHHLNYCDIVEEYE 176

Query: 130 DKMTELSRKIIKNILMSLG---EGFEKTYHEPHFKNCHGYLRINNYTAPEALQDDEEPEG 189
           + M +L+ K++   L SLG   E  E              L++N+Y       + +   G
Sbjct: 177 EHMKKLASKLMWLALNSLGVSEEDIEWASLSSDLNWAQAALQLNHY---PVCPEPDRAMG 236

Query: 190 LGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDISPFEESESERESESEALVVNIGDLLQA 249
           L  HTD + +TI+YQ+ T GLQV   +  WV + PF  S          LVVN+GDL   
Sbjct: 237 LAAHTDSTLLTILYQNNTAGLQVFRDDLGWVTVPPFPGS----------LVVNVGDLFHI 296

Query: 250 WSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKAVSAPEEVVGDGGERLYKPFVCADY 307
            SN   +S  HR  + +    R S+AF WG + +  +S   ++V      LY+     +Y
Sbjct: 297 LSNGLFKSVLHRARVNQTRA-RLSVAFLWGPQSDIKISPVPKLVSPVESPLYQSVTWKEY 339

[Close]

BLAST of Lcy01g006560 vs. ExPASy TrEMBL
Match: A0A6J1H632 (gibberellin 20-oxidase-like protein OS=Cucurbita moschata OX=3662 GN=LOC111460359 PE=3 SV=1)

HSP 1 Score: 622.9 bits (1605), Expect = 8.0e-175
Identity = 309/337 (91.69%), Postives = 318/337 (94.36%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSSLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           MSVSKASVELPILDISQPLSPSSLS LADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPHSSLNTYTPHFIASPFFESLRVSGPNFSASAQSSADFLFNQKSPHQFS 120
           SLPSETKLKIGP SSLNTYTPHFIASPFFE+LRVSGPNF ASAQSSAD LFNQKSPHQFS
Sbjct: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFS 120

Query: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFEKTYHEPHFKNCHGYLRINNYTAPEALQDDE 180
           EILQEYGDKMTELSRKIIKNILMSLGEGF+  YHE HFKNCHGYLRINNYT PE L +DE
Sbjct: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFDTKYHESHFKNCHGYLRINNYTPPETLGEDE 180

Query: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDISPFEESESERESESEALVVNIGD 240
           EPEGLGMHTDMSCVTIVYQDQTGGLQVKSS G WVDI+P +ESESE ES+SEALVVNIGD
Sbjct: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESESESDSEALVVNIGD 240

Query: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKAVSAPEEVVGDGGERLYKPFV 300
           LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEK VSAPEEVVGDGG+RLYKPFV
Sbjct: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFV 300

Query: 301 CADYLRFRENDERGKFEKVGFTVRDFAG--INKGFEE 336
           C+DYL+FRENDERGKFEKVGFTVRDFAG  I K FEE
Sbjct: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEE 337

[Close]

BLAST of Lcy01g006560 vs. ExPASy TrEMBL
Match: A0A6J1KY83 (gibberellin 20-oxidase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111499287 PE=3 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 1.9e-171
Identity = 304/337 (90.21%), Postives = 315/337 (93.47%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSSLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           MSVSKASVELPILDISQPLSPSSLS LADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPHSSLNTYTPHFIASPFFESLRVSGPNFSASAQSSADFLFNQKSPHQFS 120
           SLPSETKLKIGP SSLNTYTPHFIASPFFE+LRVSGPNF ASAQSSADFLFNQKSPHQFS
Sbjct: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADFLFNQKSPHQFS 120

Query: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFEKTYHEPHFKNCHGYLRINNYTAPEALQDDE 180
           EILQ+YGDKMTELSRKIIKNILMSLGEGF+ TYHE HFKNCHGYLRINNYT PE L +DE
Sbjct: 121 EILQDYGDKMTELSRKIIKNILMSLGEGFDTTYHESHFKNCHGYLRINNYTPPETLGEDE 180

Query: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDISPFEESESERESESEALVVNIGD 240
           EPEGLGMHTDMSCVTIVYQDQTGGLQVKSS G WVDI+P +      ESESEALVVNIGD
Sbjct: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCD------ESESEALVVNIGD 240

Query: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKAVSAPEEVVGDGGERLYKPFV 300
           LLQAWSND+LRSSEHRVVLRRPAINRFSIAFFWGFEDEK VSAPEEVVGDGG++LYKPFV
Sbjct: 241 LLQAWSNDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKKLYKPFV 300

Query: 301 CADYLRFRENDERGKFEKVGFTVRDFAG--INKGFEE 336
           C+DYL+FRENDERGKFEKVGFTVRDFAG  I K FEE
Sbjct: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEE 331

[Close]

BLAST of Lcy01g006560 vs. ExPASy TrEMBL
Match: A0A5D3BVZ1 (Putative 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G001070 PE=3 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 2.8e-167
Identity = 294/333 (88.29%), Postives = 312/333 (93.69%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSSLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           MSVSKASVELPILDISQPLSPSSLSSLADAC+KWGFFHIKNHGISK+IY+KLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYDKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPHSSLNTYTPHFIASPFFESLRVSGPNFSASAQSSADFLFNQKSPHQFS 120
           SLP ETKLKIGP SSLNTYTPHFIASPFFE+LRVSGPNF ASAQ+SA+FLFNQKS HQFS
Sbjct: 61  SLPLETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120

Query: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFEKTYHEPHFKNCHGYLRINNYTAPEALQDDE 180
           EILQEYG+KMTELSRKIIKN+L+S GEGFEK YHEPHFKNCHGYLRINNYT PE L+DDE
Sbjct: 121 EILQEYGEKMTELSRKIIKNVLLSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180

Query: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDISPFEESESERESESEALVVNIGD 240
           E EGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDI+P E        ESEALVVNIGD
Sbjct: 181 EAEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCE--------ESEALVVNIGD 240

Query: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKAVSAPEEVVGDGGERLYKPFV 300
           LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEK VSAP+E+VG+GG+R+YKPFV
Sbjct: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPKELVGEGGKRMYKPFV 300

Query: 301 CADYLRFRENDERGKFEKVGFTVRDFAG--INK 332
           C+DYL+FRENDERGKFEKVGFTVRDFAG  INK
Sbjct: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGKIINK 325

[Close]

BLAST of Lcy01g006560 vs. ExPASy TrEMBL
Match: A0A1S3C8M6 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo OX=3656 GN=LOC103497717 PE=3 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 2.8e-167
Identity = 294/333 (88.29%), Postives = 312/333 (93.69%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSSLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           MSVSKASVELPILDISQPLSPSSLSSLADAC+KWGFFHIKNHGISK+IY+KLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYDKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPHSSLNTYTPHFIASPFFESLRVSGPNFSASAQSSADFLFNQKSPHQFS 120
           SLP ETKLKIGP SSLNTYTPHFIASPFFE+LRVSGPNF ASAQ+SA+FLFNQKS HQFS
Sbjct: 61  SLPLETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120

Query: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFEKTYHEPHFKNCHGYLRINNYTAPEALQDDE 180
           EILQEYG+KMTELSRKIIKN+L+S GEGFEK YHEPHFKNCHGYLRINNYT PE L+DDE
Sbjct: 121 EILQEYGEKMTELSRKIIKNVLLSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180

Query: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDISPFEESESERESESEALVVNIGD 240
           E EGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDI+P E        ESEALVVNIGD
Sbjct: 181 EAEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCE--------ESEALVVNIGD 240

Query: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKAVSAPEEVVGDGGERLYKPFV 300
           LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEK VSAP+E+VG+GG+R+YKPFV
Sbjct: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPKELVGEGGKRMYKPFV 300

Query: 301 CADYLRFRENDERGKFEKVGFTVRDFAG--INK 332
           C+DYL+FRENDERGKFEKVGFTVRDFAG  INK
Sbjct: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGKIINK 325

[Close]

BLAST of Lcy01g006560 vs. ExPASy TrEMBL
Match: A0A0A0KB92 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G004480 PE=3 SV=1)

HSP 1 Score: 593.2 bits (1528), Expect = 6.8e-166
Identity = 290/328 (88.41%), Postives = 307/328 (93.60%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSSLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           M VSKASVELPILDISQPLSPSSLSSLADAC+KWGFFHIKNHGISK+IYNKLHSFSNEIF
Sbjct: 1   MCVSKASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKEIYNKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPHSSLNTYTPHFIASPFFESLRVSGPNFSASAQSSADFLFNQKSPHQFS 120
           +LPSETKLKIGP SS+NTYTPHFIASPFFE+LRVSGPNF ASAQ+SA+FLFNQKS HQFS
Sbjct: 61  NLPSETKLKIGPLSSVNTYTPHFIASPFFETLRVSGPNFLASAQNSAEFLFNQKSSHQFS 120

Query: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFEKTYHEPHFKNCHGYLRINNYTAPEALQDDE 180
           EILQEYGDKMTELSRKIIKNILMS GEGFEK YHEPHFKNCHGYLRINNYT PE L+DDE
Sbjct: 121 EILQEYGDKMTELSRKIIKNILMSFGEGFEKKYHEPHFKNCHGYLRINNYTPPETLEDDE 180

Query: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDISPFEESESERESESEALVVNIGD 240
           E EGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDI+P E        ESEALVVNIGD
Sbjct: 181 EVEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDINPCE--------ESEALVVNIGD 240

Query: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKAVSAPEEVVGDGGERLYKPFV 300
           LLQAWSNDR+RSSEHRVVLRRPAINRFSIAFFWGFEDEK VSAPE++V +GGER+YKPFV
Sbjct: 241 LLQAWSNDRVRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEDLVEEGGERMYKPFV 300

Query: 301 CADYLRFRENDERGKFEKVGFTVRDFAG 329
           C+DYL+FRENDERGKFEKVGFTV +FAG
Sbjct: 301 CSDYLKFRENDERGKFEKVGFTVTNFAG 320

[Close]

BLAST of Lcy01g006560 vs. NCBI nr
Match: XP_038875412.1 (gibberellin 20-oxidase-like protein [Benincasa hispida])

HSP 1 Score: 625.2 bits (1611), Expect = 3.3e-175
Identity = 308/337 (91.39%), Postives = 318/337 (94.36%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSSLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           MSVS ASVELPILDISQPLSPSSLSSLADAC+KWGFFHIKNHGISKD YNKLHSFSNEIF
Sbjct: 1   MSVSNASVELPILDISQPLSPSSLSSLADACKKWGFFHIKNHGISKDTYNKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPHSSLNTYTPHFIASPFFESLRVSGPNFSASAQSSADFLFNQKSPHQFS 120
           SLP ETKLKIGP SSLNTYTPHFIASPFFE+LRVSGPNF ASAQSSAD LFNQKSPHQFS
Sbjct: 61  SLPIETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADLLFNQKSPHQFS 120

Query: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFEKTYHEPHFKNCHGYLRINNYTAPEALQDDE 180
           EILQEYG+KMTELSRKIIKNILMS GEGFEKTYHEPHFKNCHGYLRINNYT+PE L++DE
Sbjct: 121 EILQEYGEKMTELSRKIIKNILMSFGEGFEKTYHEPHFKNCHGYLRINNYTSPETLEEDE 180

Query: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDISPFEESESERESESEALVVNIGD 240
           E EGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDI P EESE E +SESEALVVNIGD
Sbjct: 181 EAEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDIDPCEESELESKSESEALVVNIGD 240

Query: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKAVSAPEEVVGDGGERLYKPFV 300
           LLQAW NDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEK VSAPEE+VGDGGERLYKPFV
Sbjct: 241 LLQAWGNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEELVGDGGERLYKPFV 300

Query: 301 CADYLRFRENDERGKFEKVGFTVRDFA--GINKGFEE 336
           C+DYL+FRENDERGKFEKVGFTVRDFA  GI K FEE
Sbjct: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNGIKKDFEE 337

[Close]

BLAST of Lcy01g006560 vs. NCBI nr
Match: KAG7013737.1 (Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 622.9 bits (1605), Expect = 1.7e-174
Identity = 309/337 (91.69%), Postives = 318/337 (94.36%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSSLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           MSVSKASVELPILDISQPLSPSSLS LADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPHSSLNTYTPHFIASPFFESLRVSGPNFSASAQSSADFLFNQKSPHQFS 120
           SLPSETKLKIGP SSLNTYTPHFIASPFFE+LRVSGPNF ASAQSSAD LFNQKSPHQFS
Sbjct: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFS 120

Query: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFEKTYHEPHFKNCHGYLRINNYTAPEALQDDE 180
           EILQEYGDKMTELSRKIIKNILMSLGEGF+  YHE HFKNCHGYLRINNYT PE L +DE
Sbjct: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFDTKYHESHFKNCHGYLRINNYTPPETLGEDE 180

Query: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDISPFEESESERESESEALVVNIGD 240
           EPEGLGMHTDMSCVTIVYQDQTGGLQVKSS G WVDI+P +ESESE ES+SEALVVNIGD
Sbjct: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESESESDSEALVVNIGD 240

Query: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKAVSAPEEVVGDGGERLYKPFV 300
           LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEK VSAPEEVVGDGG+RLYKPFV
Sbjct: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFV 300

Query: 301 CADYLRFRENDERGKFEKVGFTVRDFAG--INKGFEE 336
           C+DYL+FRENDERGKFEKVGFTVRDFAG  I K FEE
Sbjct: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEE 337

[Close]

BLAST of Lcy01g006560 vs. NCBI nr
Match: XP_022959365.1 (gibberellin 20-oxidase-like protein [Cucurbita moschata])

HSP 1 Score: 622.9 bits (1605), Expect = 1.7e-174
Identity = 309/337 (91.69%), Postives = 318/337 (94.36%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSSLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           MSVSKASVELPILDISQPLSPSSLS LADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPHSSLNTYTPHFIASPFFESLRVSGPNFSASAQSSADFLFNQKSPHQFS 120
           SLPSETKLKIGP SSLNTYTPHFIASPFFE+LRVSGPNF ASAQSSAD LFNQKSPHQFS
Sbjct: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFS 120

Query: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFEKTYHEPHFKNCHGYLRINNYTAPEALQDDE 180
           EILQEYGDKMTELSRKIIKNILMSLGEGF+  YHE HFKNCHGYLRINNYT PE L +DE
Sbjct: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFDTKYHESHFKNCHGYLRINNYTPPETLGEDE 180

Query: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDISPFEESESERESESEALVVNIGD 240
           EPEGLGMHTDMSCVTIVYQDQTGGLQVKSS G WVDI+P +ESESE ES+SEALVVNIGD
Sbjct: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESESESDSEALVVNIGD 240

Query: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKAVSAPEEVVGDGGERLYKPFV 300
           LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEK VSAPEEVVGDGG+RLYKPFV
Sbjct: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFV 300

Query: 301 CADYLRFRENDERGKFEKVGFTVRDFAG--INKGFEE 336
           C+DYL+FRENDERGKFEKVGFTVRDFAG  I K FEE
Sbjct: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEE 337

[Close]

BLAST of Lcy01g006560 vs. NCBI nr
Match: KAG6575175.1 (Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 622.1 bits (1603), Expect = 2.8e-174
Identity = 309/337 (91.69%), Postives = 318/337 (94.36%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSSLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           MSVSKASVELPILDISQPLSPSSLS LADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPHSSLNTYTPHFIASPFFESLRVSGPNFSASAQSSADFLFNQKSPHQFS 120
           SLPSETKLKIGP SSLNTYTPHFIASPFFE+LRVSGPNF ASAQSSAD LFNQKSPHQFS
Sbjct: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFS 120

Query: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFEKTYHEPHFKNCHGYLRINNYTAPEALQDDE 180
           EILQEYGDKMTELSRKIIKNILMSLGEGF+  YHE HFKNCHGYLRINNYT PE L +DE
Sbjct: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFDTKYHESHFKNCHGYLRINNYTPPETLGEDE 180

Query: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDISPFEESESERESESEALVVNIGD 240
           EPEGLGMHTDMSCVTIVYQDQTGGLQVKSS G WVDI+P +ESESE ES+SEALVVNIGD
Sbjct: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESESESESDSEALVVNIGD 240

Query: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKAVSAPEEVVGDGGERLYKPFV 300
           LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEK VSAPEEVVGDGG+RLYKPFV
Sbjct: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFV 300

Query: 301 CADYLRFRENDERGKFEKVGFTVRDFAG--INKGFEE 336
           C+DYL+FRENDERGKFEKVGFTVRDFAG  I K FEE
Sbjct: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNAITKYFEE 337

[Close]

BLAST of Lcy01g006560 vs. NCBI nr
Match: XP_023547730.1 (gibberellin 20-oxidase-like protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 617.5 bits (1591), Expect = 7.0e-173
Identity = 308/337 (91.39%), Postives = 318/337 (94.36%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSSLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           MSVSKASVELPILDISQPLSPSSLS LADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF
Sbjct: 1   MSVSKASVELPILDISQPLSPSSLSLLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60

Query: 61  SLPSETKLKIGPHSSLNTYTPHFIASPFFESLRVSGPNFSASAQSSADFLFNQKSPHQFS 120
           SLPSETKLKIGP SSLNTYTPHFIASPFFE+LRVSGPNF ASAQSSAD LFNQKSPHQFS
Sbjct: 61  SLPSETKLKIGPLSSLNTYTPHFIASPFFETLRVSGPNFLASAQSSADSLFNQKSPHQFS 120

Query: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFEKTYHEPHFKNCHGYLRINNYTAPEALQDDE 180
           EILQEYGDKMTELSRKIIKNILMSLGEGF+ TYHE HFKNCHGYLRINNYT PE L +DE
Sbjct: 121 EILQEYGDKMTELSRKIIKNILMSLGEGFDTTYHESHFKNCHGYLRINNYTPPETLGEDE 180

Query: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDISPFEESESERESESEALVVNIGD 240
           EPEGLGMHTDMSCVTIVYQDQTGGLQVKSS G WVDI+P +ESES  ES+SEALVVNIGD
Sbjct: 181 EPEGLGMHTDMSCVTIVYQDQTGGLQVKSSAGDWVDIAPCDESES--ESDSEALVVNIGD 240

Query: 241 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKAVSAPEEVVGDGGERLYKPFV 300
           LLQAWSND+LRSSEHRVVLRRPAINRFSIAFFWGFEDEK VSAPEEVVGDGG+RLYKPFV
Sbjct: 241 LLQAWSNDKLRSSEHRVVLRRPAINRFSIAFFWGFEDEKVVSAPEEVVGDGGKRLYKPFV 300

Query: 301 CADYLRFRENDERGKFEKVGFTVRDFAG--INKGFEE 336
           C+DYL+FRENDERGKFEKVGFTVRDFAG  I K FEE
Sbjct: 301 CSDYLKFRENDERGKFEKVGFTVRDFAGNAITKDFEE 335

[Close]

BLAST of Lcy01g006560 vs. TAIR 10
Match: AT4G23340.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 382.1 bits (980), Expect = 4.6e-106
Identity = 187/331 (56.50%), Postives = 252/331 (76.13%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSSLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           MS   +S++LP+LD++QP+  S LSSL++AC++WGFF++ NHGISK++++K+ S S ++F
Sbjct: 1   MSELHSSLQLPVLDLTQPIESSILSSLSEACKEWGFFYVTNHGISKEMFSKICSLSRDVF 60

Query: 61  SLPSETKLKIGPHSSLNTYTPHFIASPFFESLRVSGPNFSASAQSSADFLFNQKSPHQFS 120
             P E+KLK+GP S    YTP +IASP+FESL VSGP+FS SA++SAD LF      +  
Sbjct: 61  KAPLESKLKLGPIS----YTPRYIASPYFESLVVSGPDFSDSAKASADVLFQDHHKPELR 120

Query: 121 EILQEYGDKMTELSRKIIKNIL-MSLGEGFEKTYHEPHFKNCHGYLRINNYTAPEALQDD 180
           E +QEYG KM ELS+++IK +L M+LG+   K  ++  F NCHGYLR+ NYT P  ++  
Sbjct: 121 ETMQEYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQ 180

Query: 181 EE-PEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDISPFEESESERESESEALVVNI 240
           EE  EGLGMHTDMSC+TIVYQD  GGLQ++S EGKW+DI+P           ++ LVVNI
Sbjct: 181 EELVEGLGMHTDMSCITIVYQDSVGGLQMRSKEGKWIDINPC----------NDFLVVNI 240

Query: 241 GDLLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKAVSAPEEVVGDGGERLYKP 300
           GDL+QAWSN RLRSSEHRVVLR+  +NR S+AFF  FEDEK + AP+E+VG+G +R YK 
Sbjct: 241 GDLMQAWSNGRLRSSEHRVVLRK-LVNRVSLAFFLCFEDEKVILAPQEIVGEGKQRSYKS 300

Query: 301 FVCADYLRFRENDERGKFEKVGFTVRDFAGI 330
           F C++YL+FR+++E GKFEK+GFTV+DFAG+
Sbjct: 301 FKCSEYLKFRQSNEEGKFEKIGFTVKDFAGL 316

[Close]

BLAST of Lcy01g006560 vs. TAIR 10
Match: AT5G51310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 366.3 bits (939), Expect = 2.6e-101
Identity = 188/334 (56.29%), Postives = 238/334 (71.26%), Query Frame = 0


Query: 7   SVELPILDISQPLSPSSLSSLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIFSLPSET 66
           S+ELP+ DIS+PLS SSL+SL DAC++WGFF++ NHG+S+D+Y KL  FS  +F L  E 
Sbjct: 2   SLELPVFDISKPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFSTGVFELEDEE 61

Query: 67  KLKIGPHSSLNTYTPHFIASPFFESLRVSGPNFSASAQSSADFLFNQKSPHQFSEILQEY 126
           K+K+G     + YTP FIASPFFESLRVSGP+F ASA+SS D   +Q +  +FS +++EY
Sbjct: 62  KMKMG----ASNYTPRFIASPFFESLRVSGPDFYASAKSSVDAFSDQATDEEFSGLMKEY 121

Query: 127 GDKMTELSRKIIKNILMSLGEGFEKTYHEPHFKNCHGYLRINNYTAPEALQDDEEP---- 186
           G+KMT+L  KI+K IL S G+     Y+E  F NCHGY RINNYT P   +DD       
Sbjct: 122 GEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNGDEQ 181

Query: 187 ---EGLGMHTDMSCVTIVYQDQTGGLQVKSSEG-KWVDISPFEESESERESESEALVVNI 246
              EGLGMHTDMSC+TIV QD  GGLQV++ +G   +DI+P          + EALVVN+
Sbjct: 182 DLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINP----------KDEALVVNV 241

Query: 247 GDLLQAWSNDRLRSSEHRVVLRRPAI--NRFSIAFFWGFEDEKAVSAPEEVVGD-GGERL 306
           GDLL AW+N RLRSS+HRV+L+R     NRFS+AFFW F+D K V AP+EVVG   G R+
Sbjct: 242 GDLLHAWTNGRLRSSQHRVILKRRGFVGNRFSLAFFWCFDDGKVVFAPDEVVGGCEGMRV 301

Query: 307 YKPFVCADYLRFRENDERGKFEKVGFTVRDFAGI 330
           ++ F C DYLRFRE++E+GKFEKVG TV DFA I
Sbjct: 302 FRSFKCGDYLRFRESNEKGKFEKVGDTVEDFARI 321

[Close]

BLAST of Lcy01g006560 vs. TAIR 10
Match: AT4G23340.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 257.7 bits (657), Expect = 1.3e-68
Identity = 124/209 (59.33%), Postives = 161/209 (77.03%), Query Frame = 0


Query: 123 LQEYGDKMTELSRKIIKNIL-MSLGEGFEKTYHEPHFKNCHGYLRINNYTAPEALQDDEE 182
           +QEYG KM ELS+++IK +L M+LG+   K  ++  F NCHGYLR+ NYT P  ++  EE
Sbjct: 1   MQEYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQEE 60

Query: 183 -PEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDISPFEESESERESESEALVVNIGD 242
             EGLGMHTDMSC+TIVYQD  GGLQ++S EGKW+DI+P           ++ LVVNIGD
Sbjct: 61  LVEGLGMHTDMSCITIVYQDSVGGLQMRSKEGKWIDINPC----------NDFLVVNIGD 120

Query: 243 LLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKAVSAPEEVVGDGGERLYKPFV 302
           L+QAWSN RLRSSEHRVVLR+  +NR S+AFF  FEDEK + AP+E+VG+G +R YK F 
Sbjct: 121 LMQAWSNGRLRSSEHRVVLRK-LVNRVSLAFFLCFEDEKVILAPQEIVGEGKQRSYKSFK 180

Query: 303 CADYLRFRENDERGKFEKVGFTVRDFAGI 330
           C++YL+FR+++E GKFEK+GFTV+DFAG+
Sbjct: 181 CSEYLKFRQSNEEGKFEKIGFTVKDFAGL 198

[Close]

BLAST of Lcy01g006560 vs. TAIR 10
Match: AT1G80330.1 (gibberellin 3-oxidase 4 )

HSP 1 Score: 132.9 bits (333), Expect = 4.9e-31
Identity = 98/317 (30.91%), Postives = 150/317 (47.32%), Query Frame = 0


Query: 1   MSVSKASVELPILDISQPLSPSSLSSLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIF 60
           +S       +P++D+S   +P   + + DA + WG F I NHGIS+ + + + S S  +F
Sbjct: 40  VSFDAVEESIPVIDLS---NPDVTTLIGDASKTWGAFQIANHGISQKLLDDIESLSKTLF 99

Query: 61  SLPSETKLKIGPHSSLNTYTPHFIASPFFESLRVSGPNFSASAQSSADFLFNQKSPH--- 120
            +PSE KL+        +       SPFFE  ++    F+ +  S  +  FN   PH   
Sbjct: 100 DMPSERKLEAASSDKGVSGYGEPRISPFFEK-KMWSEGFTIADDSYRNH-FNTLWPHDHT 159

Query: 121 QFSEILQEYGDKMTELSRKIIKNILMSLGEGFE-----KTYHEPHFKNCHGYLRINNYTA 180
           ++  I+QEY D+M +L+ +++  IL SLG   E         +   K   G +R+N+Y  
Sbjct: 160 KYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIEWAHKLEKSGSKVGRGAIRLNHY-- 219

Query: 181 PEALQDDEEPEGLGMHTDMSCVTIVYQDQTGGLQVKSSEGKWVDISPFEESESERESESE 240
                + E   GL  HTD + +TI++Q  TGGLQV   E  WV + P             
Sbjct: 220 -PVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWVTVEP----------APG 279

Query: 241 ALVVNIGDLLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKAVSAP-EEVVGDG 300
            LVVNIGDL    SN ++ S  HR  +     +R SIA+ WG        AP  ++ G  
Sbjct: 280 VLVVNIGDLFHILSNGKIPSVVHRAKVNHTR-SRISIAYLWGGPAGDVQIAPISKLTGPA 337

Query: 301 GERLYKPFVCADYLRFR 309
              LY+     +YL+ +
Sbjct: 340 EPSLYRSITWKEYLQIK 337

[Close]

BLAST of Lcy01g006560 vs. TAIR 10
Match: AT3G19010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 131.7 bits (330), Expect = 1.1e-30
Identity = 99/337 (29.38%), Postives = 157/337 (46.59%), Query Frame = 0


Query: 9   ELPILDISQPLSPSS----LSSLADACRKWGFFHIKNHGISKDIYNKLHSFSNEIFSLPS 68
           E+P++D+S+   P      +S + DAC KWGFF + NHG+  D   ++       F LP 
Sbjct: 27  EIPVIDLSRLDDPEDVQNVISEIGDACEKWGFFQVLNHGVPSDARQRVEKTVKMFFDLPM 86

Query: 69  ETKLKI------------GPHSSLNTYTPHFIASPFFESLRVSGPNFSASAQSSADFLFN 128
           E K+K+            G H+  N      +   +F+   V  P+ +         ++N
Sbjct: 87  EEKIKVKRDDVNPVGYHDGEHTK-NVKDWKEVFDIYFKDPMVI-PSTTDPEDEGLRLVYN 146

Query: 129 Q--KSPHQFSEILQEYGDKMTELSRKIIKNILMSLGEGFEKTYHEPHFKNCHGYLRINNY 188
           +  +SP  F E  + Y     +L+ K+++ I +SLG   E+ +H+ +FK    + RIN Y
Sbjct: 147 KWPQSPSDFREACEVYARHAEKLAFKLLELISLSLGLPKER-FHD-YFKEQMSFFRINRY 206

Query: 189 TAPEALQDDEEPEGLGMHTDMSCVTIVYQDQTGGLQV-KSSEGKWVDISPFEESESERES 248
             P   + D    G+G H D   ++++ QD  GGLQV + S+G W  I P          
Sbjct: 207 --PPCPRPD-LALGVGHHKDADVISLLAQDDVGGLQVSRRSDGVWFPIRPV--------- 266

Query: 249 ESEALVVNIGDLLQAWSNDRLRSSEHRVVLRRPAINRFSIAFFWGFEDEKAVSAPEEVVG 308
              ALV+NIG+ ++ W+ND+  S+EHRVV+      R+SI FF     +  V   EE+V 
Sbjct: 267 -PNALVINIGNCMEIWTNDKYWSAEHRVVVNTTR-ERYSIPFFLLPSHDVEVKPLEELVS 326

Query: 309 DGGERLYKPFVCADYLRFRENDERGKFEKVGFTVRDF 327
                 YK +    +   R   +  K E     + DF
Sbjct: 327 PENPPKYKGYKWGKFYVSRNRSDFRKLEIENIQIDDF 345

The following BLAST results are available for this feature:

BLAST of Lcy01g006560 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4KBY0	3.7e-100	56.29	Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310...	[more]
Q9C971	6.8e-30	30.91	Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 ...	[more]
Q9LHN8	5.8e-29	28.89	Feruloyl CoA ortho-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=F6'H1 PE=1 S...	[more]
Q9C899	2.2e-28	31.37	Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=F6'H2 PE=1 S...	[more]
Q39103	1.2e-26	29.14	Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 ...	[more]

BLAST of Lcy01g006560 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H632	8.0e-175	91.69	gibberellin 20-oxidase-like protein OS=Cucurbita moschata OX=3662 GN=LOC11146035...	[more]
A0A6J1KY83	1.9e-171	90.21	gibberellin 20-oxidase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111499287 ...	[more]
A0A5D3BVZ1	2.8e-167	88.29	Putative 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo var. makuwa O...	[more]
A0A1S3C8M6	2.8e-167	88.29	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A0A0KB92	6.8e-166	88.41	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G...	[more]

BLAST of Lcy01g006560 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038875412.1	3.3e-175	91.39	gibberellin 20-oxidase-like protein [Benincasa hispida]	[more]
KAG7013737.1	1.7e-174	91.69	Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. argy...	[more]
XP_022959365.1	1.7e-174	91.69	gibberellin 20-oxidase-like protein [Cucurbita moschata]	[more]
KAG6575175.1	2.8e-174	91.69	Gibberellin 20-oxidase-like protein, partial [Cucurbita argyrosperma subsp. soro...	[more]
XP_023547730.1	7.0e-173	91.39	gibberellin 20-oxidase-like protein [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lcy01g006560 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G23340.1	4.6e-106	56.50	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G51310.1	2.6e-101	56.29	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G23340.2	1.3e-68	59.33	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G80330.1	4.9e-31	30.91	gibberellin 3-oxidase 4	[more]
AT3G19010.1	1.1e-30	29.38	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 166..272 e-value: 1.4E-18 score: 67.2
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..307 e-value: 6.3E-69 score: 235.0
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 10..75 e-value: 1.8E-15 score: 57.7
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 4..326
None	No IPR available	PANTHER	PTHR47990:SF52	GIBBERELLIN 3-BETA-DIOXYGENASE 4	coord: 4..326
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 7..311
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 161..275 score: 11.076853

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy01g006560.1	Lcy01g006560.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1901576	organic substance biosynthetic process
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen