Lcy01g005740 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy01g005740
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Calcium-binding EF-hand
Location	Chr01: 8074286 .. 8075323 (+)
RNA-Seq Expression	Lcy01g005740
Synteny	Lcy01g005740

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCAATCAAAGAGCCGTAGAACATTAAGACATTTTCTTCTACACTCTCCCAACTATTTCCTTTCACCATCATCTCTCAATCAAAATTTTCACCAAGTACAATAATTTTGTTCTCTCTTTTCTCTCAAGTTATGTTCAATTCTCTTGTATCTTCCTTCGCCAACTTCCATAGAAAAATCACCTCCAAACCCTTCTTCCAAAAAACCCTAAACAACCAAAAGCTCACCACTTTTAACCACTTTGAGCTCCTCTTCAAGCTCCTTGACTCCAATGGTGATGGCAAGATCTCAGCCAAAGAGCTTTGTCAATTCCTCCATCGTTTAGGTTACAAGAAGCTGAAAGCCGCAAAAGAAGCCGAAGAAATGGTGAAGGAAATGGATTTCGACCGTGATGGATTCATCGACATGGATGAATTTTTAGGGTGAGTCTGGCATACACTCTAGTTTGAGCACTTTTGTAAAACGTATACTCTTTTTTCTAAAAAAAAACCCGACATAGAAGAGTAGTGTTTAAATTCTATCGATCACAAATTTGTTTAATTGTATTGGATTCGAGAATCAGTTATGTCAAACACACTCTTAGTCGATCGTAATAACGAAATCTTCAAAACCTAATAACGAAATCTTCAATAGGGTTTTGAAGGAGGAAGAAGATGATGATGTTCTTATGGAAGCTTTTTTGATATTTGATGCTGACAAGAATGGCTTGATTTGTGCTGAAGAACTAAGGAGAACATTGAGCAAATTTGGGTGGAGAAAATGTGGGTTGAAAGAATGCAAAAGAATGATTGAAGGAGTGGATAAAGATGGCGATGGGTTTGTGAATTTTCAAGATTTTCGGCTGATGATGACAGCAAGAGATGACGGATTTTTTAGTTGAGATTTAATTATCAATTTGATTGATATGTGTGAGAAAAGTCTTCTCTTGTTAATGGTTATGATCATGACTTGTAATATATCTTCTTTTTCTTACACTCCCCCCTTTTTAATTGTAATAATGGATTCAATTTTTCTCGTTAATTAAATAACGAATTCGGTCA

mRNA sequence

CTCAATCAAAGAGCCGTAGAACATTAAGACATTTTCTTCTACACTCTCCCAACTATTTCCTTTCACCATCATCTCTCAATCAAAATTTTCACCAAGTACAATAATTTTGTTCTCTCTTTTCTCTCAAGTTATGTTCAATTCTCTTGTATCTTCCTTCGCCAACTTCCATAGAAAAATCACCTCCAAACCCTTCTTCCAAAAAACCCTAAACAACCAAAAGCTCACCACTTTTAACCACTTTGAGCTCCTCTTCAAGCTCCTTGACTCCAATGGTGATGGCAAGATCTCAGCCAAAGAGCTTTGTCAATTCCTCCATCGTTTAGGTTACAAGAAGCTGAAAGCCGCAAAAGAAGCCGAAGAAATGGTGAAGGAAATGGATTTCGACCGTGATGGATTCATCGACATGGATGAATTTTTAGGGGTTTTGAAGGAGGAAGAAGATGATGATGTTCTTATGGAAGCTTTTTTGATATTTGATGCTGACAAGAATGGCTTGATTTGTGCTGAAGAACTAAGGAGAACATTGAGCAAATTTGGGTGGAGAAAATGTGGGTTGAAAGAATGCAAAAGAATGATTGAAGGAGTGGATAAAGATGGCGATGGGTTTGTGAATTTTCAAGATTTTCGGCTGATGATGACAGCAAGAGATGACGGATTTTTTAGTTGAGATTTAATTATCAATTTGATTGATATGTGTGAGAAAAGTCTTCTCTTGTTAATGGTTATGATCATGACTTGTAATATATCTTCTTTTTCTTACACTCCCCCCTTTTTAATTGTAATAATGGATTCAATTTTTCTCGTTAATTAAATAACGAATTCGGTCA

Coding sequence (CDS)

ATGTTCAATTCTCTTGTATCTTCCTTCGCCAACTTCCATAGAAAAATCACCTCCAAACCCTTCTTCCAAAAAACCCTAAACAACCAAAAGCTCACCACTTTTAACCACTTTGAGCTCCTCTTCAAGCTCCTTGACTCCAATGGTGATGGCAAGATCTCAGCCAAAGAGCTTTGTCAATTCCTCCATCGTTTAGGTTACAAGAAGCTGAAAGCCGCAAAAGAAGCCGAAGAAATGGTGAAGGAAATGGATTTCGACCGTGATGGATTCATCGACATGGATGAATTTTTAGGGGTTTTGAAGGAGGAAGAAGATGATGATGTTCTTATGGAAGCTTTTTTGATATTTGATGCTGACAAGAATGGCTTGATTTGTGCTGAAGAACTAAGGAGAACATTGAGCAAATTTGGGTGGAGAAAATGTGGGTTGAAAGAATGCAAAAGAATGATTGAAGGAGTGGATAAAGATGGCGATGGGTTTGTGAATTTTCAAGATTTTCGGCTGATGATGACAGCAAGAGATGACGGATTTTTTAGTTGA

Protein sequence

MFNSLVSSFANFHRKITSKPFFQKTLNNQKLTTFNHFELLFKLLDSNGDGKISAKELCQFLHRLGYKKLKAAKEAEEMVKEMDFDRDGFIDMDEFLGVLKEEEDDDVLMEAFLIFDADKNGLICAEELRRTLSKFGWRKCGLKECKRMIEGVDKDGDGFVNFQDFRLMMTARDDGFFS

Homology

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BLAST of Lcy01g005740 vs. ExPASy Swiss-Prot
Match: Q9FYK2 (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 3.3e-23
Identity = 64/155 (41.29%), Postives = 96/155 (61.94%), Query Frame = 0


Query: 20  PFFQKTLNNQKLTTFNHFELLFKLLDSNGDGKISAKELCQFLHRLGYKKLKAAKEAEEMV 79
           P+ QK  + +  T     E +FK  D NGDGKIS+KEL   +  LG++     +E E+ +
Sbjct: 23  PYLQKARSGK--TEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHE--VPEEELEKAI 82

Query: 80  KEMDFDRDGFIDMDEFLGV-LKEEEDDDV---LMEAFLIFDADKNGLICAEELRRTLSKF 139
            E+D   DG+I+ +EF+ +  K  + +DV   L +AF ++D D NG I AEEL   L   
Sbjct: 83  TEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSL 142

Query: 140 GWRKCGLKECKRMIEGVDKDGDGFVNFQDFRLMMT 171
           G  +C + EC++MI GVDKDGDG ++F++F++MMT
Sbjct: 143 G-DECSIAECRKMIGGVDKDGDGTIDFEEFKIMMT 172

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BLAST of Lcy01g005740 vs. ExPASy Swiss-Prot
Match: Q0DJV6 (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 GN=CML18 PE=2 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.2e-22
Identity = 66/148 (44.59%), Postives = 83/148 (56.08%), Query Frame = 0


Query: 38  ELLFKLLDSNGDGKISAKELCQFLHRLGYKKLKAAKEAEEMVKEMDFDRDGFIDMDEFL- 97
           E +F+  D+NGDGKISA+EL   L  LG        E   M+ EMD DRDGF+D+ EF  
Sbjct: 7   EQVFRRYDANGDGKISAEELASVLRALGAP--LGPGEVRRMMDEMDSDRDGFVDLSEFAA 66

Query: 98  -------------GVLKEEE--DDDVLMEAFLIFDADKNGLICAEELRRTLSKFGWRKCG 157
                        G  K++E   +  L EAF ++DAD NG I A EL R L + G  KC 
Sbjct: 67  FHCGPTPAHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLG-DKCS 126

Query: 158 LKECKRMIEGVDKDGDGFVNFQDFRLMM 170
           + +C RMI  VD DGDG VNF +F+ MM
Sbjct: 127 VADCSRMIRSVDADGDGCVNFDEFKKMM 151

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BLAST of Lcy01g005740 vs. ExPASy Swiss-Prot
Match: Q9LE22 (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 PE=1 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 1.4e-21
Identity = 56/132 (42.42%), Postives = 82/132 (62.12%), Query Frame = 0


Query: 40  LFKLLDSNGDGKISAKELCQFLHRLGYKKLKAAKEAEEMVKEMDFDRDGFIDMDEFLGVL 99
           +F   DSNGDGKIS  EL      +G    +   E   +++E+D DRDG+I++DEF  + 
Sbjct: 27  VFDQFDSNGDGKISVLELGGVFKAMGTSYTET--ELNRVLEEVDTDRDGYINLDEFSTLC 86

Query: 100 KEEEDDDVLMEAFLIFDADKNGLICAEELRRTLSKFGWRKCGLKECKRMIEGVDKDGDGF 159
           +       + +AF ++D DKNGLI A EL + L++ G   C +++C RMI  VD DGDG 
Sbjct: 87  RSSSSAAEIRDAFDLYDQDKNGLISASELHQVLNRLG-MSCSVEDCTRMIGPVDADGDGN 146

Query: 160 VNFQDFRLMMTA 172
           VNF++F+ MMT+
Sbjct: 147 VNFEEFQKMMTS 155

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BLAST of Lcy01g005740 vs. ExPASy Swiss-Prot
Match: Q9SRR7 (Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 2.4e-21
Identity = 54/135 (40.00%), Postives = 90/135 (66.67%), Query Frame = 0


Query: 40  LFKLLDSNGDGKISAKELCQFLHRLGYKKLKAAKEAEEMVKEMDFDRDGFIDMDEFLG-- 99
           +F++ D NGDGKI+ +EL   L  LG       K+  +M++++D + DG++D++EF G  
Sbjct: 9   IFQMFDRNGDGKITKQELNDSLENLGI--YIPDKDLVQMIEKIDLNGDGYVDIEEFGGLY 68

Query: 100 --VLKEEEDDDVLMEAFLIFDADKNGLICAEELRRTLSKFGWRK-CGLKECKRMIEGVDK 159
             +++E ++++ + EAF +FD +++G I  EELR  L+  G ++   L++CKRMI  VD 
Sbjct: 69  QTIMEERDEEEDMREAFNVFDQNRDGFITVEELRSVLASLGLKQGRTLEDCKRMISKVDV 128

Query: 160 DGDGFVNFQDFRLMM 170
           DGDG VNF++F+ MM
Sbjct: 129 DGDGMVNFKEFKQMM 141

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BLAST of Lcy01g005740 vs. ExPASy Swiss-Prot
Match: Q9M7R0 (Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 1.2e-20
Identity = 59/143 (41.26%), Postives = 86/143 (60.14%), Query Frame = 0


Query: 35  NHFELLFKLLDSNGDGKISAKELCQFLHRLGYKKLKAAKEAEEMVKEMDFDRDGFIDMDE 94
           N  + +F   D+NGDGKIS  EL   L  LG    K  +E   +++E+D D+DGFI++ E
Sbjct: 19  NEVQGVFNRFDANGDGKISGDELAGVLKALGSNTSK--EEIGRIMEEIDTDKDGFINVQE 78

Query: 95  FLGVLKEEED-------DDVLMEAFLIFDADKNGLICAEELRRTLSKFGWRKCGLKECKR 154
           F   +K E D       ++ L EAF ++D D NGLI + EL + L++ G R     +C  
Sbjct: 79  FAAFVKAETDPYPSSGGENELKEAFELYDQDHNGLISSVELHKILTRLGERYAE-HDCVE 138

Query: 155 MIEGVDKDGDGFVNFQDFRLMMT 171
           MI+ VD DGDG+V+F++F+ MMT
Sbjct: 139 MIKSVDSDGDGYVSFEEFKKMMT 158

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BLAST of Lcy01g005740 vs. ExPASy TrEMBL
Match: A0A0A0K8Z4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G076830 PE=4 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 8.6e-67
Identity = 135/177 (76.27%), Postives = 152/177 (85.88%), Query Frame = 0


Query: 1   MFNSLVSSFAN-FHRKITSKPFFQKTLNNQKL---TTFNHFELLFKLLDSNGDGKISAKE 60
           MFN   S F   FHR I+SKPFFQK+LNNQKL   TTFNHF+LLF LLDS+GDGKIS KE
Sbjct: 1   MFNLTSSLFPTFFHRNISSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKISTKE 60

Query: 61  LCQFLHRLGYKKLKAAKEAEEMVKEMDFDRDGFIDMDEFLGVL-KEEEDDDVLMEAFLIF 120
           L QFL+RLGYKKLKA  EAEEMVKEMD DRDGFI+MDEFL VL K EE++++L EAFLIF
Sbjct: 61  LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF 120

Query: 121 DADKNGLICAEELRRTLSKFGWRKCGLKECKRMIEGVDKDGDGFVNFQDFRLMMTAR 173
           D DKNGLICAE+L+RTLSKFGW+KC LK+CK+MIEGVD DGDG+VNFQDFRLMMT +
Sbjct: 121 DGDKNGLICAEKLKRTLSKFGWQKCDLKQCKKMIEGVDHDGDGYVNFQDFRLMMTQK 177

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BLAST of Lcy01g005740 vs. ExPASy TrEMBL
Match: A0A1S3CBX8 (calmodulin-like protein 7 OS=Cucumis melo OX=3656 GN=LOC103499032 PE=4 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 1.9e-66
Identity = 134/174 (77.01%), Postives = 149/174 (85.63%), Query Frame = 0


Query: 3   NSLVSSFANFHRKITSKPFFQKTLNNQKL---TTFNHFELLFKLLDSNGDGKISAKELCQ 62
           +SL S+F  FHR ITSKPFFQK+LNNQKL   TTFNHF+LLF +LDS GDGKIS KEL Q
Sbjct: 6   SSLFSTF--FHRNITSKPFFQKSLNNQKLPSFTTFNHFQLLFNILDSGGDGKISTKELSQ 65

Query: 63  FLHRLGYKKLKAAKEAEEMVKEMDFDRDGFIDMDEFLGVL-KEEEDDDVLMEAFLIFDAD 122
           FL+RLGYKKLKA  EAEEMVKEMD DRDGFI+MDEFL VL K EE++++L EAFLIFD D
Sbjct: 66  FLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIFDGD 125

Query: 123 KNGLICAEELRRTLSKFGWRKCGLKECKRMIEGVDKDGDGFVNFQDFRLMMTAR 173
           KNGLICAEEL+RT  KFGW KC LK+CK+MIEGVD DGDGFVNFQDFRLMMT +
Sbjct: 126 KNGLICAEELKRTFRKFGWTKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMTQK 177

[Close]

BLAST of Lcy01g005740 vs. ExPASy TrEMBL
Match: A0A067JXS4 (Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_14512 PE=4 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 2.2e-38
Identity = 86/168 (51.19%), Postives = 119/168 (70.83%), Query Frame = 0


Query: 5   LVSSFANFHRKITSKPF-FQKTLNNQKLTTFNHFELLFKLLDSNGDGKISAKELCQFLHR 64
           +++      R ++S  F F  T +   +   N F+ +F L+D+NGDGKIS+ +L + L  
Sbjct: 16  VIAKLTKHKRNLSSSTFDFSDTPSFASMEIPNQFKQVFNLIDANGDGKISSYDLIELLLC 75

Query: 65  LGYKKLKAAKEAEEMVKEMDFDRDGFIDMDEFLGVLKEEEDDDVLMEAFLIFDADKNGLI 124
           LG  K KA  EAE MVKE+D + DGF+D DEF  +L   +++D LM+AF+IFDADKNGLI
Sbjct: 76  LGCDKSKAKAEAEGMVKELDRNGDGFVDFDEFFYILNNYDEEDYLMDAFMIFDADKNGLI 135

Query: 125 CAEELRRTLSKFGWRKCGLKECKRMIEGVDKDGDGFVNFQDFRLMMTA 172
            A+EL++ LS+ G+ KC LKEC+ MI+GVDKDGDGFV+F++FR MMTA
Sbjct: 136 SAKELQKVLSRLGFEKCSLKECRIMIKGVDKDGDGFVDFEEFRSMMTA 183

[Close]

BLAST of Lcy01g005740 vs. ExPASy TrEMBL
Match: A0A1R3I7M2 (Calcium-binding EF-hand OS=Corchorus capsularis OX=210143 GN=CCACVL1_14273 PE=4 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 3.8e-38
Identity = 86/149 (57.72%), Postives = 110/149 (73.83%), Query Frame = 0


Query: 35  NHFELLFKLLDSNGDGKISAKELCQFLHRLGYKKLKAAKEAEEMVKEMDFDRDGFIDMDE 94
           N F  +FK++D+NGDGKIS  EL + L  LG++K  A+K AE MVKEMDF+ DGFID+DE
Sbjct: 5   NQFRHVFKVIDANGDGKISPLELSEVLLCLGHEKSTASKAAEGMVKEMDFNGDGFIDLDE 64

Query: 95  FLGVLKEEE------------DDDVLMEAFLIFDADKNGLICAEELRRTLSKFGWRKCGL 154
           F+ V+  +E            ++D LM+AFLIFD DKNGLI A+ELR+ L   G+ KC L
Sbjct: 65  FMHVVHSDEAICGSNGGGGGGEEDCLMDAFLIFDTDKNGLISAKELRKVLISLGFTKCSL 124

Query: 155 KECKRMIEGVDKDGDGFVNFQDFRLMMTA 172
           +ECKRMI+GVDKDGDGFV+F++FR MMTA
Sbjct: 125 QECKRMIKGVDKDGDGFVDFKEFRFMMTA 153

[Close]

BLAST of Lcy01g005740 vs. ExPASy TrEMBL
Match: A0A2Z6MNS5 (Uncharacterized protein OS=Trifolium subterraneum OX=3900 GN=TSUD_363200 PE=4 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 1.4e-37
Identity = 80/144 (55.56%), Postives = 105/144 (72.92%), Query Frame = 0


Query: 37  FELLFKLLDSNGDGKISAKELCQFLHRLGYKKLKAAKEAEEMVKEMDFDRDGFIDMDEFL 96
           F  +FKL+D+NGDGKIS  ELC+ L  LGY K  A KEAE M+K +DF+ DGF+D+DEF+
Sbjct: 7   FRQVFKLIDTNGDGKISTTELCEMLSALGYNKYTATKEAENMIKALDFNGDGFVDIDEFM 66

Query: 97  GVLKEE--------EDDDVLMEAFLIFDADKNGLICAEELRRTLSKFGWRKCGLKECKRM 156
            V+KE+        + D+ LM+AFL+FD+DKNGLI  +ELR  L   G   C L+ECKRM
Sbjct: 67  FVMKEDSYGKGKEHDHDEYLMDAFLVFDSDKNGLISPKELRSVLVNLGCDNCSLRECKRM 126

Query: 157 IEGVDKDGDGFVNFQDFRLMMTAR 173
           I+GVDK+GDGFV+F++FR MM  +
Sbjct: 127 IKGVDKNGDGFVDFEEFRSMMNLK 150

[Close]

BLAST of Lcy01g005740 vs. NCBI nr
Match: XP_004153117.1 (calmodulin-beta [Cucumis sativus] >KGN46235.1 hypothetical protein Csa_005220 [Cucumis sativus])

HSP 1 Score: 263.1 bits (671), Expect = 1.8e-66
Identity = 135/177 (76.27%), Postives = 152/177 (85.88%), Query Frame = 0


Query: 1   MFNSLVSSFAN-FHRKITSKPFFQKTLNNQKL---TTFNHFELLFKLLDSNGDGKISAKE 60
           MFN   S F   FHR I+SKPFFQK+LNNQKL   TTFNHF+LLF LLDS+GDGKIS KE
Sbjct: 1   MFNLTSSLFPTFFHRNISSKPFFQKSLNNQKLPSFTTFNHFQLLFNLLDSDGDGKISTKE 60

Query: 61  LCQFLHRLGYKKLKAAKEAEEMVKEMDFDRDGFIDMDEFLGVL-KEEEDDDVLMEAFLIF 120
           L QFL+RLGYKKLKA  EAEEMVKEMD DRDGFI+MDEFL VL K EE++++L EAFLIF
Sbjct: 61  LSQFLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIF 120

Query: 121 DADKNGLICAEELRRTLSKFGWRKCGLKECKRMIEGVDKDGDGFVNFQDFRLMMTAR 173
           D DKNGLICAE+L+RTLSKFGW+KC LK+CK+MIEGVD DGDG+VNFQDFRLMMT +
Sbjct: 121 DGDKNGLICAEKLKRTLSKFGWQKCDLKQCKKMIEGVDHDGDGYVNFQDFRLMMTQK 177

[Close]

BLAST of Lcy01g005740 vs. NCBI nr
Match: XP_008460131.1 (PREDICTED: calmodulin-like protein 7 [Cucumis melo])

HSP 1 Score: 261.9 bits (668), Expect = 3.9e-66
Identity = 134/174 (77.01%), Postives = 149/174 (85.63%), Query Frame = 0


Query: 3   NSLVSSFANFHRKITSKPFFQKTLNNQKL---TTFNHFELLFKLLDSNGDGKISAKELCQ 62
           +SL S+F  FHR ITSKPFFQK+LNNQKL   TTFNHF+LLF +LDS GDGKIS KEL Q
Sbjct: 6   SSLFSTF--FHRNITSKPFFQKSLNNQKLPSFTTFNHFQLLFNILDSGGDGKISTKELSQ 65

Query: 63  FLHRLGYKKLKAAKEAEEMVKEMDFDRDGFIDMDEFLGVL-KEEEDDDVLMEAFLIFDAD 122
           FL+RLGYKKLKA  EAEEMVKEMD DRDGFI+MDEFL VL K EE++++L EAFLIFD D
Sbjct: 66  FLYRLGYKKLKATMEAEEMVKEMDSDRDGFIEMDEFLEVLQKGEEEEEILREAFLIFDGD 125

Query: 123 KNGLICAEELRRTLSKFGWRKCGLKECKRMIEGVDKDGDGFVNFQDFRLMMTAR 173
           KNGLICAEEL+RT  KFGW KC LK+CK+MIEGVD DGDGFVNFQDFRLMMT +
Sbjct: 126 KNGLICAEELKRTFRKFGWTKCDLKQCKKMIEGVDHDGDGFVNFQDFRLMMTQK 177

[Close]

BLAST of Lcy01g005740 vs. NCBI nr
Match: XP_012083557.1 (probable calcium-binding protein CML27 [Jatropha curcas] >KDP28741.1 hypothetical protein JCGZ_14512 [Jatropha curcas])

HSP 1 Score: 168.7 bits (426), Expect = 4.5e-38
Identity = 86/168 (51.19%), Postives = 119/168 (70.83%), Query Frame = 0


Query: 5   LVSSFANFHRKITSKPF-FQKTLNNQKLTTFNHFELLFKLLDSNGDGKISAKELCQFLHR 64
           +++      R ++S  F F  T +   +   N F+ +F L+D+NGDGKIS+ +L + L  
Sbjct: 16  VIAKLTKHKRNLSSSTFDFSDTPSFASMEIPNQFKQVFNLIDANGDGKISSYDLIELLLC 75

Query: 65  LGYKKLKAAKEAEEMVKEMDFDRDGFIDMDEFLGVLKEEEDDDVLMEAFLIFDADKNGLI 124
           LG  K KA  EAE MVKE+D + DGF+D DEF  +L   +++D LM+AF+IFDADKNGLI
Sbjct: 76  LGCDKSKAKAEAEGMVKELDRNGDGFVDFDEFFYILNNYDEEDYLMDAFMIFDADKNGLI 135

Query: 125 CAEELRRTLSKFGWRKCGLKECKRMIEGVDKDGDGFVNFQDFRLMMTA 172
            A+EL++ LS+ G+ KC LKEC+ MI+GVDKDGDGFV+F++FR MMTA
Sbjct: 136 SAKELQKVLSRLGFEKCSLKECRIMIKGVDKDGDGFVDFEEFRSMMTA 183

[Close]

BLAST of Lcy01g005740 vs. NCBI nr
Match: OMO78596.1 (Calcium-binding EF-hand [Corchorus capsularis])

HSP 1 Score: 167.9 bits (424), Expect = 7.7e-38
Identity = 86/149 (57.72%), Postives = 110/149 (73.83%), Query Frame = 0


Query: 35  NHFELLFKLLDSNGDGKISAKELCQFLHRLGYKKLKAAKEAEEMVKEMDFDRDGFIDMDE 94
           N F  +FK++D+NGDGKIS  EL + L  LG++K  A+K AE MVKEMDF+ DGFID+DE
Sbjct: 5   NQFRHVFKVIDANGDGKISPLELSEVLLCLGHEKSTASKAAEGMVKEMDFNGDGFIDLDE 64

Query: 95  FLGVLKEEE------------DDDVLMEAFLIFDADKNGLICAEELRRTLSKFGWRKCGL 154
           F+ V+  +E            ++D LM+AFLIFD DKNGLI A+ELR+ L   G+ KC L
Sbjct: 65  FMHVVHSDEAICGSNGGGGGGEEDCLMDAFLIFDTDKNGLISAKELRKVLISLGFTKCSL 124

Query: 155 KECKRMIEGVDKDGDGFVNFQDFRLMMTA 172
           +ECKRMI+GVDKDGDGFV+F++FR MMTA
Sbjct: 125 QECKRMIKGVDKDGDGFVDFKEFRFMMTA 153

[Close]

BLAST of Lcy01g005740 vs. NCBI nr
Match: GAU25150.1 (hypothetical protein TSUD_363200 [Trifolium subterraneum])

HSP 1 Score: 166.0 bits (419), Expect = 2.9e-37
Identity = 80/144 (55.56%), Postives = 105/144 (72.92%), Query Frame = 0


Query: 37  FELLFKLLDSNGDGKISAKELCQFLHRLGYKKLKAAKEAEEMVKEMDFDRDGFIDMDEFL 96
           F  +FKL+D+NGDGKIS  ELC+ L  LGY K  A KEAE M+K +DF+ DGF+D+DEF+
Sbjct: 7   FRQVFKLIDTNGDGKISTTELCEMLSALGYNKYTATKEAENMIKALDFNGDGFVDIDEFM 66

Query: 97  GVLKEE--------EDDDVLMEAFLIFDADKNGLICAEELRRTLSKFGWRKCGLKECKRM 156
            V+KE+        + D+ LM+AFL+FD+DKNGLI  +ELR  L   G   C L+ECKRM
Sbjct: 67  FVMKEDSYGKGKEHDHDEYLMDAFLVFDSDKNGLISPKELRSVLVNLGCDNCSLRECKRM 126

Query: 157 IEGVDKDGDGFVNFQDFRLMMTAR 173
           I+GVDK+GDGFV+F++FR MM  +
Sbjct: 127 IKGVDKNGDGFVDFEEFRSMMNLK 150

[Close]

BLAST of Lcy01g005740 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 109.8 bits (273), Expect = 2.3e-24
Identity = 64/155 (41.29%), Postives = 96/155 (61.94%), Query Frame = 0


Query: 20  PFFQKTLNNQKLTTFNHFELLFKLLDSNGDGKISAKELCQFLHRLGYKKLKAAKEAEEMV 79
           P+ QK  + +  T     E +FK  D NGDGKIS+KEL   +  LG++     +E E+ +
Sbjct: 23  PYLQKARSGK--TEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHE--VPEEELEKAI 82

Query: 80  KEMDFDRDGFIDMDEFLGV-LKEEEDDDV---LMEAFLIFDADKNGLICAEELRRTLSKF 139
            E+D   DG+I+ +EF+ +  K  + +DV   L +AF ++D D NG I AEEL   L   
Sbjct: 83  TEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSL 142

Query: 140 GWRKCGLKECKRMIEGVDKDGDGFVNFQDFRLMMT 171
           G  +C + EC++MI GVDKDGDG ++F++F++MMT
Sbjct: 143 G-DECSIAECRKMIGGVDKDGDGTIDFEEFKIMMT 172

[Close]

BLAST of Lcy01g005740 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 104.4 bits (259), Expect = 9.8e-23
Identity = 56/132 (42.42%), Postives = 82/132 (62.12%), Query Frame = 0


Query: 40  LFKLLDSNGDGKISAKELCQFLHRLGYKKLKAAKEAEEMVKEMDFDRDGFIDMDEFLGVL 99
           +F   DSNGDGKIS  EL      +G    +   E   +++E+D DRDG+I++DEF  + 
Sbjct: 27  VFDQFDSNGDGKISVLELGGVFKAMGTSYTET--ELNRVLEEVDTDRDGYINLDEFSTLC 86

Query: 100 KEEEDDDVLMEAFLIFDADKNGLICAEELRRTLSKFGWRKCGLKECKRMIEGVDKDGDGF 159
           +       + +AF ++D DKNGLI A EL + L++ G   C +++C RMI  VD DGDG 
Sbjct: 87  RSSSSAAEIRDAFDLYDQDKNGLISASELHQVLNRLG-MSCSVEDCTRMIGPVDADGDGN 146

Query: 160 VNFQDFRLMMTA 172
           VNF++F+ MMT+
Sbjct: 147 VNFEEFQKMMTS 155

[Close]

BLAST of Lcy01g005740 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 104.4 bits (259), Expect = 9.8e-23
Identity = 56/132 (42.42%), Postives = 82/132 (62.12%), Query Frame = 0


Query: 40  LFKLLDSNGDGKISAKELCQFLHRLGYKKLKAAKEAEEMVKEMDFDRDGFIDMDEFLGVL 99
           +F   DSNGDGKIS  EL      +G    +   E   +++E+D DRDG+I++DEF  + 
Sbjct: 27  VFDQFDSNGDGKISVLELGGVFKAMGTSYTET--ELNRVLEEVDTDRDGYINLDEFSTLC 86

Query: 100 KEEEDDDVLMEAFLIFDADKNGLICAEELRRTLSKFGWRKCGLKECKRMIEGVDKDGDGF 159
           +       + +AF ++D DKNGLI A EL + L++ G   C +++C RMI  VD DGDG 
Sbjct: 87  RSSSSAAEIRDAFDLYDQDKNGLISASELHQVLNRLG-MSCSVEDCTRMIGPVDADGDGN 146

Query: 160 VNFQDFRLMMTA 172
           VNF++F+ MMT+
Sbjct: 147 VNFEEFQKMMTS 155

[Close]

BLAST of Lcy01g005740 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 103.6 bits (257), Expect = 1.7e-22
Identity = 54/135 (40.00%), Postives = 90/135 (66.67%), Query Frame = 0


Query: 40  LFKLLDSNGDGKISAKELCQFLHRLGYKKLKAAKEAEEMVKEMDFDRDGFIDMDEFLG-- 99
           +F++ D NGDGKI+ +EL   L  LG       K+  +M++++D + DG++D++EF G  
Sbjct: 9   IFQMFDRNGDGKITKQELNDSLENLGI--YIPDKDLVQMIEKIDLNGDGYVDIEEFGGLY 68

Query: 100 --VLKEEEDDDVLMEAFLIFDADKNGLICAEELRRTLSKFGWRK-CGLKECKRMIEGVDK 159
             +++E ++++ + EAF +FD +++G I  EELR  L+  G ++   L++CKRMI  VD 
Sbjct: 69  QTIMEERDEEEDMREAFNVFDQNRDGFITVEELRSVLASLGLKQGRTLEDCKRMISKVDV 128

Query: 160 DGDGFVNFQDFRLMM 170
           DGDG VNF++F+ MM
Sbjct: 129 DGDGMVNFKEFKQMM 141

[Close]

BLAST of Lcy01g005740 vs. TAIR 10
Match: AT3G10190.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 101.3 bits (251), Expect = 8.3e-22
Identity = 62/141 (43.97%), Postives = 82/141 (58.16%), Query Frame = 0


Query: 34  FNHFELL--FKLLDSNGDGKISAKELCQFLHRLGYKKLKAAKEAEEMVKEMDFDRDGFID 93
           +++ E+L  FKL+D + DG +S  +L   L RLG   L   +E   M+KE+D D DG I 
Sbjct: 66  YSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPL-TEEEINVMLKEVDCDGDGTIR 125

Query: 94  MDEFLG---VLKEEEDDDVLMEAFLIFDADKNGLICAEELRRTLSKFGWRKCGLKECKRM 153
           ++E       L    D   L E F  FDAD++GLI A+EL R  S  G  +C L +CKRM
Sbjct: 126 LEELASRVVSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDERCTLDDCKRM 185

Query: 154 IEGVDKDGDGFVNFQDFRLMM 170
           I  VD+DGDGFV F +F  MM
Sbjct: 186 IADVDEDGDGFVCFTEFSRMM 205

The following BLAST results are available for this feature:

BLAST of Lcy01g005740 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FYK2	3.3e-23	41.29	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ...	[more]
Q0DJV6	1.2e-22	44.59	Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9LE22	1.4e-21	42.42	Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 ...	[more]
Q9SRR7	2.4e-21	40.00	Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1	[more]
Q9M7R0	1.2e-20	41.26	Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1	[more]

BLAST of Lcy01g005740 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K8Z4	8.6e-67	76.27	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G076830 PE=4 SV=1	[more]
A0A1S3CBX8	1.9e-66	77.01	calmodulin-like protein 7 OS=Cucumis melo OX=3656 GN=LOC103499032 PE=4 SV=1	[more]
A0A067JXS4	2.2e-38	51.19	Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_14512 PE=4 SV=1	[more]
A0A1R3I7M2	3.8e-38	57.72	Calcium-binding EF-hand OS=Corchorus capsularis OX=210143 GN=CCACVL1_14273 PE=4 ...	[more]
A0A2Z6MNS5	1.4e-37	55.56	Uncharacterized protein OS=Trifolium subterraneum OX=3900 GN=TSUD_363200 PE=4 SV...	[more]

BLAST of Lcy01g005740 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004153117.1	1.8e-66	76.27	calmodulin-beta [Cucumis sativus] >KGN46235.1 hypothetical protein Csa_005220 [C...	[more]
XP_008460131.1	3.9e-66	77.01	PREDICTED: calmodulin-like protein 7 [Cucumis melo]	[more]
XP_012083557.1	4.5e-38	51.19	probable calcium-binding protein CML27 [Jatropha curcas] >KDP28741.1 hypothetica...	[more]
OMO78596.1	7.7e-38	57.72	Calcium-binding EF-hand [Corchorus capsularis]	[more]
GAU25150.1	2.9e-37	55.56	hypothetical protein TSUD_363200 [Trifolium subterraneum]	[more]

BLAST of Lcy01g005740 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G24620.1	2.3e-24	41.29	EF hand calcium-binding protein family	[more]
AT1G18210.1	9.8e-23	42.42	Calcium-binding EF-hand family protein	[more]
AT1G18210.2	9.8e-23	42.42	Calcium-binding EF-hand family protein	[more]
AT3G07490.1	1.7e-22	40.00	ARF-GAP domain 11	[more]
AT3G10190.1	8.3e-22	43.97	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 144..172 e-value: 3.2E-5 score: 33.3 coord: 74..102 e-value: 0.088 score: 21.9 coord: 36..64 e-value: 8.1E-5 score: 32.0 coord: 107..135 e-value: 0.0015 score: 27.8
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 106..169 e-value: 1.3E-8 score: 35.1 coord: 39..97 e-value: 1.0E-11 score: 45.1
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 70..100 score: 9.57592
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 32..67 score: 14.513405
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 103..138 score: 13.369694
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 140..175 score: 11.891237
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 36..96 e-value: 2.10594E-12 score: 57.5577
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 108..170 e-value: 3.36411E-13 score: 59.4837
None	No IPR available	GENE3D	1.10.238.10		coord: 99..176 e-value: 2.2E-18 score: 68.3
None	No IPR available	GENE3D	1.10.238.10		coord: 4..98 e-value: 3.3E-17 score: 64.5
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 27..171
None	No IPR available	PANTHER	PTHR23050:SF382	CALCIUM-BINDING PROTEIN CML25-RELATED	coord: 27..171
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 153..165
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 116..128
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 45..57
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 32..170

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy01g005740.1	Lcy01g005740.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0065007	biological regulation
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005509	calcium ion binding