Lcy01g004700 (gene) Sponge gourd (P93075) v1

Overview
NameLcy01g004700
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionEnt-kaurene oxidase
LocationChr01: 6515938 .. 6516307 (-)
RNA-Seq ExpressionLcy01g004700
SyntenyLcy01g004700
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGACGAGATATTCCTCCATTTCCAGTAGAAAGCTAAGCAAAGCCCTCACCATTTTGACTTCTGATAAATGCATGGTTGCTATGAGTGATTACAATGACTTTCACAAGTTGGTGAAGAGATATATATTGGCTAATCTCTTGGGTGTTAATGCTCAGGTGGTTTCTAATTTCTTTTTCTTCTCTTCATTTGGGTCAATTCTAATAATTTGATCTGTTTAATTTTCAGAAGAGACATAGACAAAGGAGAGACACCATGATGGAGAATATTTGTAGCCAGTTTTTTGCTCAGGTGAAGGATTCTCCTTCAGAATCTGTTAATTTTCGAAAGATCTTCGAAGCCGAACTCTTTTGGCTGGCGCTTAAAGAA

mRNA sequence

ATGGTGACGAGATATTCCTCCATTTCCAGTAGAAAGCTAAGCAAAGCCCTCACCATTTTGACTTCTGATAAATGCATGGTTGCTATGAGTGATTACAATGACTTTCACAAGTTGGTGAAGAGATATATATTGGCTAATCTCTTGGGTGTTAATGCTCAGAAGAGACATAGACAAAGGAGAGACACCATGATGGAGAATATTTGTAGCCAGTTTTTTGCTCAGGTGAAGGATTCTCCTTCAGAATCTGTTAATTTTCGAAAGATCTTCGAAGCCGAACTCTTTTGGCTGGCGCTTAAAGAA

Coding sequence (CDS)

ATGGTGACGAGATATTCCTCCATTTCCAGTAGAAAGCTAAGCAAAGCCCTCACCATTTTGACTTCTGATAAATGCATGGTTGCTATGAGTGATTACAATGACTTTCACAAGTTGGTGAAGAGATATATATTGGCTAATCTCTTGGGTGTTAATGCTCAGAAGAGACATAGACAAAGGAGAGACACCATGATGGAGAATATTTGTAGCCAGTTTTTTGCTCAGGTGAAGGATTCTCCTTCAGAATCTGTTAATTTTCGAAAGATCTTCGAAGCCGAACTCTTTTGGCTGGCGCTTAAAGAA

Protein sequence

MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQKRHRQRRDTMMENICSQFFAQVKDSPSESVNFRKIFEAELFWLALKE
Homology
BLAST of Lcy01g004700 vs. ExPASy Swiss-Prot
Match: A0A0G2RKY1 (Ent-kaurene oxidase OS=Salvia miltiorrhiza OX=226208 GN=KO PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 3.5e-30
Identity = 65/100 (65.00%), Postives = 82/100 (82.00%), Query Frame = 0

Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQKRHRQRR 60
           M+T+YSSIS+RKLSKALTILTSDK +VAMSDYN+F+K  KR++L + LG  AQKRHR  R
Sbjct: 105 MITKYSSISTRKLSKALTILTSDKSIVAMSDYNEFYKAAKRHLLTSTLGPTAQKRHRVHR 164

Query: 61  DTMMENICSQFFAQVKDSPSESVNFRKIFEAELFWLALKE 101
           + M+ NIC QF A  K  PSE+VNFRKIF++ELF L++K+
Sbjct: 165 NLMINNICDQFLAHAKMYPSEAVNFRKIFQSELFGLSMKQ 204

BLAST of Lcy01g004700 vs. ExPASy Swiss-Prot
Match: Q93ZB2 (Ent-kaurene oxidase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KO PE=1 SV=2)

HSP 1 Score: 126.3 bits (316), Expect = 1.9e-28
Identity = 65/100 (65.00%), Postives = 77/100 (77.00%), Query Frame = 0

Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQKRHRQRR 60
           MVTR+SSIS+RKLS ALT+LT DK MVA SDY+DFHKLVKR +L  LLG NAQKR R  R
Sbjct: 101 MVTRFSSISTRKLSNALTVLTCDKSMVATSDYDDFHKLVKRCLLNGLLGANAQKRKRHYR 160

Query: 61  DTMMENICSQFFAQVKDSPSESVNFRKIFEAELFWLALKE 101
           D ++EN+ S+  A  +D P E VNFR IFE ELF +ALK+
Sbjct: 161 DALIENVSSKLHAHARDHPQEPVNFRAIFEHELFGVALKQ 200

BLAST of Lcy01g004700 vs. ExPASy Swiss-Prot
Match: C7J3A2 (Ent-kaurene oxidase-like protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A7 PE=5 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 1.1e-23
Identity = 55/98 (56.12%), Postives = 73/98 (74.49%), Query Frame = 0

Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQKRHRQRR 60
           MV ++SSIS+RKLSKALT+LT DK MVA SDY DFHK+VKRY++++ LG +AQK+ R  R
Sbjct: 109 MVAKFSSISTRKLSKALTVLTRDKSMVATSDYGDFHKMVKRYVMSSTLGTSAQKKFRDTR 168

Query: 61  DTMMENICSQFFAQVKDSPSESVNFRKIFEAELFWLAL 99
           D M+ N+ S F   VKD P   + FR +F+ ELF L++
Sbjct: 169 DMMINNMLSTFHKLVKDDPHVPLIFRDVFKDELFRLSM 206

BLAST of Lcy01g004700 vs. ExPASy Swiss-Prot
Match: Q5Z5R4 (Ent-kaurene oxidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A6 PE=1 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 1.8e-23
Identity = 55/98 (56.12%), Postives = 74/98 (75.51%), Query Frame = 0

Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQKRHRQRR 60
           MV ++SSIS+RKLSKALT+LT DK MVA SDY DFHK+VKRY+++++LG +AQK+ R  R
Sbjct: 98  MVAKFSSISTRKLSKALTVLTRDKSMVATSDYCDFHKMVKRYVMSSMLGTSAQKQFRDIR 157

Query: 61  DTMMENICSQFFAQVKDSPSESVNFRKIFEAELFWLAL 99
           D M+ N+ S F   VKD P   + FR +F+ ELF L++
Sbjct: 158 DMMIHNMLSTFHKLVKDDPHAPLIFRDVFKDELFRLSM 195

BLAST of Lcy01g004700 vs. ExPASy Swiss-Prot
Match: Q5Z5R7 (Ent-kaurene oxidase-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A19 PE=2 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 3.8e-21
Identity = 52/98 (53.06%), Postives = 71/98 (72.45%), Query Frame = 0

Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQKRHRQRR 60
           MV ++SSIS+RKLSKALT+L+ DK MVA SD  DFHK+ KRYI+ ++LG +AQK+ R  R
Sbjct: 97  MVAKFSSISTRKLSKALTVLSHDKSMVATSDSGDFHKMGKRYIMLSMLGTSAQKQFRDTR 156

Query: 61  DTMMENICSQFFAQVKDSPSESVNFRKIFEAELFWLAL 99
           D ++ N+ S F   VKD P   + FR +F+ ELF L++
Sbjct: 157 DMIINNMLSTFHQLVKDDPHAPLIFRDVFKDELFRLSM 194

BLAST of Lcy01g004700 vs. ExPASy TrEMBL
Match: A0A6J1F7C8 (ent-kaurene oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111442772 PE=3 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.5e-39
Identity = 86/100 (86.00%), Postives = 95/100 (95.00%), Query Frame = 0

Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQKRHRQRR 60
           MVTRY+S+SSRKLSKALTILTSDKCMVAMSDYNDFHK+VK+YILAN+LG NAQKRHRQRR
Sbjct: 112 MVTRYASMSSRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGANAQKRHRQRR 171

Query: 61  DTMMENICSQFFAQVKDSPSESVNFRKIFEAELFWLALKE 101
           DTM++NI SQ FA VKDSPSESVNFR+IFE+ELF LALKE
Sbjct: 172 DTMIDNISSQLFAHVKDSPSESVNFRRIFESELFRLALKE 211

BLAST of Lcy01g004700 vs. ExPASy TrEMBL
Match: A0A6J1IL06 (ent-kaurene oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111476578 PE=3 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 4.2e-39
Identity = 85/100 (85.00%), Postives = 94/100 (94.00%), Query Frame = 0

Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQKRHRQRR 60
           MVTRY+S+S RKLSKALTILTSDKCMVAMSDYNDFHK+VK+YILAN+LG NAQKRHRQRR
Sbjct: 112 MVTRYASMSGRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGANAQKRHRQRR 171

Query: 61  DTMMENICSQFFAQVKDSPSESVNFRKIFEAELFWLALKE 101
           D+M++NI SQ FA VKDSPSESVNFRKIFE+ELF LALKE
Sbjct: 172 DSMIDNISSQLFAHVKDSPSESVNFRKIFESELFLLALKE 211

BLAST of Lcy01g004700 vs. ExPASy TrEMBL
Match: A0A6J1CGM6 (ent-kaurene oxidase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111010641 PE=3 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 9.5e-39
Identity = 86/100 (86.00%), Postives = 91/100 (91.00%), Query Frame = 0

Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQKRHRQRR 60
           MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHK+ KRYILAN+LG NAQKRHRQRR
Sbjct: 112 MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKMAKRYILANVLGANAQKRHRQRR 171

Query: 61  DTMMENICSQFFAQVKDSPSESVNFRKIFEAELFWLALKE 101
           DTM+ENI S  FA  KDSPS+SVNFR+IFE ELF LALKE
Sbjct: 172 DTMIENISSHLFAHAKDSPSQSVNFREIFELELFRLALKE 211

BLAST of Lcy01g004700 vs. ExPASy TrEMBL
Match: A0A6J1J3Y4 (ent-kaurene oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111481118 PE=3 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 1.8e-37
Identity = 86/100 (86.00%), Postives = 92/100 (92.00%), Query Frame = 0

Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQKRHRQRR 60
           MVTRYSSISSRKLSKALTILT+DKCMVAMSDYNDFHKLVKRYILAN+LG NAQKR RQRR
Sbjct: 110 MVTRYSSISSRKLSKALTILTADKCMVAMSDYNDFHKLVKRYILANVLGANAQKRLRQRR 169

Query: 61  DTMMENICSQFFAQVKDSPSESVNFRKIFEAELFWLALKE 101
           DTM++NI  + FA VKDS SESVNFRKIFE+ELF LALKE
Sbjct: 170 DTMIDNISRELFACVKDSSSESVNFRKIFESELFGLALKE 209

BLAST of Lcy01g004700 vs. ExPASy TrEMBL
Match: Q9FQY5 (Ent-kaurene oxidase OS=Cucurbita maxima OX=3661 GN=CYP701A1 PE=2 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 1.8e-37
Identity = 86/100 (86.00%), Postives = 92/100 (92.00%), Query Frame = 0

Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQKRHRQRR 60
           MVTRYSSISSRKLSKALTILT+DKCMVAMSDYNDFHKLVKRYILAN+LG NAQKR RQRR
Sbjct: 110 MVTRYSSISSRKLSKALTILTADKCMVAMSDYNDFHKLVKRYILANVLGANAQKRLRQRR 169

Query: 61  DTMMENICSQFFAQVKDSPSESVNFRKIFEAELFWLALKE 101
           DTM++NI  + FA VKDS SESVNFRKIFE+ELF LALKE
Sbjct: 170 DTMIDNISRELFACVKDSSSESVNFRKIFESELFGLALKE 209

BLAST of Lcy01g004700 vs. NCBI nr
Match: KAG6591351.1 (Ent-kaurene oxidase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 171.8 bits (434), Expect = 3.0e-39
Identity = 86/100 (86.00%), Postives = 95/100 (95.00%), Query Frame = 0

Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQKRHRQRR 60
           MVTRY+S+SSRKLSKALTILTSDKCMVAMSDYNDFHK+VK+YILAN+LG NAQKRHRQRR
Sbjct: 112 MVTRYASMSSRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGANAQKRHRQRR 171

Query: 61  DTMMENICSQFFAQVKDSPSESVNFRKIFEAELFWLALKE 101
           DTM++NI SQ FA VKDSPSESVNFR+IFE+ELF LALKE
Sbjct: 172 DTMIDNISSQLFAHVKDSPSESVNFRRIFESELFRLALKE 211

BLAST of Lcy01g004700 vs. NCBI nr
Match: KAG7024228.1 (Ent-kaurene oxidase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 171.8 bits (434), Expect = 3.0e-39
Identity = 86/100 (86.00%), Postives = 95/100 (95.00%), Query Frame = 0

Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQKRHRQRR 60
           MVTRY+S+SSRKLSKALTILTSDKCMVAMSDYNDFHK+VK+YILAN+LG NAQKRHRQRR
Sbjct: 112 MVTRYASMSSRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGANAQKRHRQRR 171

Query: 61  DTMMENICSQFFAQVKDSPSESVNFRKIFEAELFWLALKE 101
           DTM++NI SQ FA VKDSPSESVNFR+IFE+ELF LALKE
Sbjct: 172 DTMIDNISSQLFAHVKDSPSESVNFRRIFESELFRLALKE 211

BLAST of Lcy01g004700 vs. NCBI nr
Match: XP_022936057.1 (ent-kaurene oxidase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 171.8 bits (434), Expect = 3.0e-39
Identity = 86/100 (86.00%), Postives = 95/100 (95.00%), Query Frame = 0

Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQKRHRQRR 60
           MVTRY+S+SSRKLSKALTILTSDKCMVAMSDYNDFHK+VK+YILAN+LG NAQKRHRQRR
Sbjct: 112 MVTRYASMSSRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGANAQKRHRQRR 171

Query: 61  DTMMENICSQFFAQVKDSPSESVNFRKIFEAELFWLALKE 101
           DTM++NI SQ FA VKDSPSESVNFR+IFE+ELF LALKE
Sbjct: 172 DTMIDNISSQLFAHVKDSPSESVNFRRIFESELFRLALKE 211

BLAST of Lcy01g004700 vs. NCBI nr
Match: XP_022976063.1 (ent-kaurene oxidase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 170.2 bits (430), Expect = 8.8e-39
Identity = 85/100 (85.00%), Postives = 94/100 (94.00%), Query Frame = 0

Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQKRHRQRR 60
           MVTRY+S+S RKLSKALTILTSDKCMVAMSDYNDFHK+VK+YILAN+LG NAQKRHRQRR
Sbjct: 112 MVTRYASMSGRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGANAQKRHRQRR 171

Query: 61  DTMMENICSQFFAQVKDSPSESVNFRKIFEAELFWLALKE 101
           D+M++NI SQ FA VKDSPSESVNFRKIFE+ELF LALKE
Sbjct: 172 DSMIDNISSQLFAHVKDSPSESVNFRKIFESELFLLALKE 211

BLAST of Lcy01g004700 vs. NCBI nr
Match: XP_023536271.1 (ent-kaurene oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 169.5 bits (428), Expect = 1.5e-38
Identity = 85/100 (85.00%), Postives = 95/100 (95.00%), Query Frame = 0

Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQKRHRQRR 60
           MVTR++S+SSRKLSKALTILTSDKCMVAMSDYNDFHK+VK+YILAN+LG NAQKRHRQRR
Sbjct: 112 MVTRHASMSSRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGANAQKRHRQRR 171

Query: 61  DTMMENICSQFFAQVKDSPSESVNFRKIFEAELFWLALKE 101
           D+M++NI SQ FA VKDSPSESVNFRKIFE+ELF LALKE
Sbjct: 172 DSMIDNISSQLFAHVKDSPSESVNFRKIFESELFRLALKE 211

BLAST of Lcy01g004700 vs. TAIR 10
Match: AT5G25900.1 (GA requiring 3 )

HSP 1 Score: 126.3 bits (316), Expect = 1.4e-29
Identity = 65/100 (65.00%), Postives = 77/100 (77.00%), Query Frame = 0

Query: 1   MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKLVKRYILANLLGVNAQKRHRQRR 60
           MVTR+SSIS+RKLS ALT+LT DK MVA SDY+DFHKLVKR +L  LLG NAQKR R  R
Sbjct: 101 MVTRFSSISTRKLSNALTVLTCDKSMVATSDYDDFHKLVKRCLLNGLLGANAQKRKRHYR 160

Query: 61  DTMMENICSQFFAQVKDSPSESVNFRKIFEAELFWLALKE 101
           D ++EN+ S+  A  +D P E VNFR IFE ELF +ALK+
Sbjct: 161 DALIENVSSKLHAHARDHPQEPVNFRAIFEHELFGVALKQ 200

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A0G2RKY13.5e-3065.00Ent-kaurene oxidase OS=Salvia miltiorrhiza OX=226208 GN=KO PE=1 SV=1[more]
Q93ZB21.9e-2865.00Ent-kaurene oxidase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KO PE=1 SV... [more]
C7J3A21.1e-2356.12Ent-kaurene oxidase-like protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=C... [more]
Q5Z5R41.8e-2356.12Ent-kaurene oxidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A6 PE=1 ... [more]
Q5Z5R73.8e-2153.06Ent-kaurene oxidase-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A19... [more]
Match NameE-valueIdentityDescription
A0A6J1F7C81.5e-3986.00ent-kaurene oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1IL064.2e-3985.00ent-kaurene oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111476... [more]
A0A6J1CGM69.5e-3986.00ent-kaurene oxidase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC11101064... [more]
A0A6J1J3Y41.8e-3786.00ent-kaurene oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111481... [more]
Q9FQY51.8e-3786.00Ent-kaurene oxidase OS=Cucurbita maxima OX=3661 GN=CYP701A1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KAG6591351.13.0e-3986.00Ent-kaurene oxidase, chloroplastic, partial [Cucurbita argyrosperma subsp. soror... [more]
KAG7024228.13.0e-3986.00Ent-kaurene oxidase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyr... [more]
XP_022936057.13.0e-3986.00ent-kaurene oxidase, chloroplastic-like [Cucurbita moschata][more]
XP_022976063.18.8e-3985.00ent-kaurene oxidase, chloroplastic-like [Cucurbita maxima][more]
XP_023536271.11.5e-3885.00ent-kaurene oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G25900.11.4e-2965.00GA requiring 3 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR044225Ent-kaurene oxidase, chloroplasticPANTHERPTHR47283ENT-KAURENE OXIDASE, CHLOROPLASTICcoord: 1..100

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy01g004700.1Lcy01g004700.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010241 ent-kaurene oxidation to kaurenoic acid
biological_process GO:0009686 gibberellin biosynthetic process
cellular_component GO:0009707 chloroplast outer membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0052615 ent-kaurene oxidase activity
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding