Lcy01g004630 (gene) Sponge gourd (P93075) v1

Overview
NameLcy01g004630
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionSerine hydroxymethyltransferase
LocationChr01: 6392374 .. 6395246 (-)
RNA-Seq ExpressionLcy01g004630
SyntenyLcy01g004630
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCCCCCATTTCCAGCTTCGTTCTTCTTCTTCTTCGTTCTTCATCTCCAAATTCTCTGTAATTTCTCTCTCTCTCTCGTCCGCCATGGATCCCGTCAAAGCTTGGGGCAACACTCCTCTCGAAACCGTCGACCCTGAGATCTTCGATCTCATCGAGAAGGAGAAGCGTCGCCAATGCCGCGGAATCGAGCTCATTGCCTCTGAGAATTTCACTTCCTTCGCTGTCATCGAAGCCCTCGGCAGCGCCTTGACCAACAAGTACTCCGAAGGCATGCCGGGGAATCGATACTACGGTGGCAACGAGTTCATCGACGAGATCGAGAATCTCTGCCGTTCTCGAGCTCTCGAGGCCTTCCATGTCGATCCTGCCAAGTGGGGTGTCAATGTCCAGCCCTACTCTGGTTCTCCGGCTAATTTCGCCGCCTACACCGCGTTGCTTCAGCCGCATGACCGGATTATGGGGCTGGATCTTCCTTCTGGTGGCCATTTGACGCATGGTTACTACACCTCCGGTGGGAAGAAGATCTCCGCTACCTCGATTTACTTTGAGAGTTTGCCTTACAAGGTTGATTCCGCTACTGGATATATCGATTACGATAAGCTGGAGGAGAAGGCTTTGGATTTCAGGCCCAAGTTGATTATCTGTGGTGGCAGTGCCTACCCTAGAGACTGGGATTACGCCAGATTCAGGGCAATTGCCGACAAATGCGGTGCCCTTCTGCTTTGTGATATGGCTCACATTAGTGGACTCGTTGCTGCTCAGGTATAAAAATGAAATAAATCTATGAATCTGATTGTCGAGTTTCAGATCTTTTGTGTCATTCTTGTATATCATGAAGAATGTTGGCAATTGGATGATGTTTGTTTTCCATTTCTGGATTTGGGATCTGTTGGTTTACGTTGAATTTGCTTTTCTCTTTGATCTCTGCAGGTGATATGCTCAATGTGTTCATACTTGTCTTTAGTATCTGTGTTCAGTGCATTGATTGAAAATGGTTGGAAATGACACAGATTTTTAGATCCATAATTGTTTGAGCATCTTACGTAACAGGATCTGGCTTTTTGAAAACTTATTTAATGATTAATATCGATGCTGCATGCCAACCAGTTCAAATCCCCACTCCCACATGTTGTTGAACTAGAAAAATAATAATAATTATTATCAATGCTGCATTGCTTCTTTGTAACGGAAGATAAATTGTGTTCTTTGGATTAGTTGAACTGTTATGTTTTTTATTGTTTTTTGCAAATATTTTGTATTCCTTGTCTTTACTATATGATTCAGTTTTAAATGCTTAGTTGAAAATTGAAATGAAGATCTACTTAGAGGAAATGGATACATTTCTGCTTCTAGTTGAATTGAGTTTTAACGTAAAACGAATCCTGTGTTCAATCATCTTCATGTTTTGATCTTACATTACGTTTTTCTTCAAAAATTATTCAAAGGCATATTTCTTGTATGACATTCTGTTTGTTTACGATCTCTGTTTCTTTCCATGCAACTTAAAGTGATCTGGGTCTGGCTGGTATTGTAAAATAGTAATGTCCATTGATTGTTTGCAGGAAGCTGCAAACCCGTTCGAGTATTGTGACGTTGTGACGACCACAACTCACAAGAGTTTGAGGGGTCCCAGGGCTGGTATGATCTTCTATAGGAAGGGTCCAAAACCACCTAAGAAGGGTCAGCCTGAGGATGCTGTTTATGACTTTGAAGACAAGATCAACTTCTCTGTCTTCCCAGCCCTTCAAGGTGGCCCTCACAATCACCAAATTGGTGCTTTAGCTGTTGCCTTGAAGCAGGCTATGTCCCCTGGGTTCAAGACCTATGCAAAACAAGTCAAGGCCAATGCCGTGGCCCTCGGAAATTACCTGATGAGCAAGGGCTACAAGCTTGTCACGGGAGGAACCGAGAACCACCTTGTTCTCTGGGATCTTCGTCCTTTGGGATTGACTGGTAATTTAGCACGAATCAACTTCAGAAAACTAACAGAGAAGACATGTTCTGTTCTGTTCTGTTCGTGTAACTGTTTTGTGGTTGATTCCTTTTGCAGGCAACAAGGTAGAGAAGCTTTGTGACTTGTGCAACATTACTGTAAACAAGAATGCCGTGTTCGGCGACAGCAGTGCATTGGCTCCCGGAGGTGTTCGAATTGGTAAGGGCCGATATCTCTAGAAGACGATTCCTTCATCCACAGTCCTTTCATTAACTTGCTTTCCCTGCAATCAATCTATGAGTAGCTATCAATTTTGAATATGTGATTAACAAGATACCAAATTGTGATGATAGGTGCACCTGCCATGACATCGAGAGGTTTGGTCGAAAAAGACTTCGAACAGATCGCAGAGTTCCTACACCGTGCTGTAACCATCACCTTGAGCATCCAGAAGGAATACGGAAAGCTGTTGAAGGACTTCAACAAGGGTCTGGTAAACAACAAGGACATCGAAGAGCTGAAGGCCGACGTCGAGAAATTCTCTGGCTCATTCGACATGCCCGGTTTCCTCATGTCCGAGATGAAGTACAAGGACTAGAAAGTAAAGAAACAAGATCGTCTTGAGCAAACTAATAAAGTTTTGGGTGTTTGTTGAATCTTTAAAATCTTTTCACTTCCAAAGTTGAAAAAGTAAAAAGAAAAGGTTGTTGTTTGTTGGTTATCTTTTTTTTCTTTTTCCTTATTTGTATGATGTAGACTAGATTTTCCTTCGAAAATATTCATTCGAGAAAGTTTCATGCTAAGAAAAGAATGAGTTCGAATTTTCGTATATCAATCACTGTATTTTTTATAAAAAAATATATATATCCATCATTTTGTGCGTGAATTTGATAAGCATATTTGAAGGTTTGGGCTTGATAGGTCGTGAAGCGGCCCAATTT

mRNA sequence

GCCCCCATTTCCAGCTTCGTTCTTCTTCTTCTTCGTTCTTCATCTCCAAATTCTCTGTAATTTCTCTCTCTCTCTCGTCCGCCATGGATCCCGTCAAAGCTTGGGGCAACACTCCTCTCGAAACCGTCGACCCTGAGATCTTCGATCTCATCGAGAAGGAGAAGCGTCGCCAATGCCGCGGAATCGAGCTCATTGCCTCTGAGAATTTCACTTCCTTCGCTGTCATCGAAGCCCTCGGCAGCGCCTTGACCAACAAGTACTCCGAAGGCATGCCGGGGAATCGATACTACGGTGGCAACGAGTTCATCGACGAGATCGAGAATCTCTGCCGTTCTCGAGCTCTCGAGGCCTTCCATGTCGATCCTGCCAAGTGGGGTGTCAATGTCCAGCCCTACTCTGGTTCTCCGGCTAATTTCGCCGCCTACACCGCGTTGCTTCAGCCGCATGACCGGATTATGGGGCTGGATCTTCCTTCTGGTGGCCATTTGACGCATGGTTACTACACCTCCGGTGGGAAGAAGATCTCCGCTACCTCGATTTACTTTGAGAGTTTGCCTTACAAGGTTGATTCCGCTACTGGATATATCGATTACGATAAGCTGGAGGAGAAGGCTTTGGATTTCAGGCCCAAGTTGATTATCTGTGGTGGCAGTGCCTACCCTAGAGACTGGGATTACGCCAGATTCAGGGCAATTGCCGACAAATGCGGTGCCCTTCTGCTTTGTGATATGGCTCACATTAGTGGACTCGTTGCTGCTCAGGAAGCTGCAAACCCGTTCGAGTATTGTGACGTTGTGACGACCACAACTCACAAGAGTTTGAGGGGTCCCAGGGCTGGTATGATCTTCTATAGGAAGGGTCCAAAACCACCTAAGAAGGGTCAGCCTGAGGATGCTGTTTATGACTTTGAAGACAAGATCAACTTCTCTGTCTTCCCAGCCCTTCAAGGTGGCCCTCACAATCACCAAATTGGTGCTTTAGCTGTTGCCTTGAAGCAGGCTATGTCCCCTGGGTTCAAGACCTATGCAAAACAAGTCAAGGCCAATGCCGTGGCCCTCGGAAATTACCTGATGAGCAAGGGCTACAAGCTTGTCACGGGAGGAACCGAGAACCACCTTGTTCTCTGGGATCTTCGTCCTTTGGGATTGACTGGCAACAAGGTAGAGAAGCTTTGTGACTTGTGCAACATTACTGTAAACAAGAATGCCGTGTTCGGCGACAGCAGTGCATTGGCTCCCGGAGGTGTTCGAATTGGTGCACCTGCCATGACATCGAGAGGTTTGGTCGAAAAAGACTTCGAACAGATCGCAGAGTTCCTACACCGTGCTGTAACCATCACCTTGAGCATCCAGAAGGAATACGGAAAGCTGTTGAAGGACTTCAACAAGGGTCTGGTAAACAACAAGGACATCGAAGAGCTGAAGGCCGACGTCGAGAAATTCTCTGGCTCATTCGACATGCCCGGTTTCCTCATGTCCGAGATGAAGTACAAGGACTAGAAAGTAAAGAAACAAGATCGTCTTGAGCAAACTAATAAAGTTTTGGGTGTTTGTTGAATCTTTAAAATCTTTTCACTTCCAAAGTTGAAAAAGTAAAAAGAAAAGGTTGTTGTTTGTTGGTTATCTTTTTTTTCTTTTTCCTTATTTGTATGATGTAGACTAGATTTTCCTTCGAAAATATTCATTCGAGAAAGTTTCATGCTAAGAAAAGAATGAGTTCGAATTTTCGTATATCAATCACTGTATTTTTTATAAAAAAATATATATATCCATCATTTTGTGCGTGAATTTGATAAGCATATTTGAAGGTTTGGGCTTGATAGGTCGTGAAGCGGCCCAATTT

Coding sequence (CDS)

ATGGATCCCGTCAAAGCTTGGGGCAACACTCCTCTCGAAACCGTCGACCCTGAGATCTTCGATCTCATCGAGAAGGAGAAGCGTCGCCAATGCCGCGGAATCGAGCTCATTGCCTCTGAGAATTTCACTTCCTTCGCTGTCATCGAAGCCCTCGGCAGCGCCTTGACCAACAAGTACTCCGAAGGCATGCCGGGGAATCGATACTACGGTGGCAACGAGTTCATCGACGAGATCGAGAATCTCTGCCGTTCTCGAGCTCTCGAGGCCTTCCATGTCGATCCTGCCAAGTGGGGTGTCAATGTCCAGCCCTACTCTGGTTCTCCGGCTAATTTCGCCGCCTACACCGCGTTGCTTCAGCCGCATGACCGGATTATGGGGCTGGATCTTCCTTCTGGTGGCCATTTGACGCATGGTTACTACACCTCCGGTGGGAAGAAGATCTCCGCTACCTCGATTTACTTTGAGAGTTTGCCTTACAAGGTTGATTCCGCTACTGGATATATCGATTACGATAAGCTGGAGGAGAAGGCTTTGGATTTCAGGCCCAAGTTGATTATCTGTGGTGGCAGTGCCTACCCTAGAGACTGGGATTACGCCAGATTCAGGGCAATTGCCGACAAATGCGGTGCCCTTCTGCTTTGTGATATGGCTCACATTAGTGGACTCGTTGCTGCTCAGGAAGCTGCAAACCCGTTCGAGTATTGTGACGTTGTGACGACCACAACTCACAAGAGTTTGAGGGGTCCCAGGGCTGGTATGATCTTCTATAGGAAGGGTCCAAAACCACCTAAGAAGGGTCAGCCTGAGGATGCTGTTTATGACTTTGAAGACAAGATCAACTTCTCTGTCTTCCCAGCCCTTCAAGGTGGCCCTCACAATCACCAAATTGGTGCTTTAGCTGTTGCCTTGAAGCAGGCTATGTCCCCTGGGTTCAAGACCTATGCAAAACAAGTCAAGGCCAATGCCGTGGCCCTCGGAAATTACCTGATGAGCAAGGGCTACAAGCTTGTCACGGGAGGAACCGAGAACCACCTTGTTCTCTGGGATCTTCGTCCTTTGGGATTGACTGGCAACAAGGTAGAGAAGCTTTGTGACTTGTGCAACATTACTGTAAACAAGAATGCCGTGTTCGGCGACAGCAGTGCATTGGCTCCCGGAGGTGTTCGAATTGGTGCACCTGCCATGACATCGAGAGGTTTGGTCGAAAAAGACTTCGAACAGATCGCAGAGTTCCTACACCGTGCTGTAACCATCACCTTGAGCATCCAGAAGGAATACGGAAAGCTGTTGAAGGACTTCAACAAGGGTCTGGTAAACAACAAGGACATCGAAGAGCTGAAGGCCGACGTCGAGAAATTCTCTGGCTCATTCGACATGCCCGGTTTCCTCATGTCCGAGATGAAGTACAAGGACTAG

Protein sequence

MDPVKAWGNTPLETVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQPHDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDFRPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALAVALKQAMSPGFKTYAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITLSIQKEYGKLLKDFNKGLVNNKDIEELKADVEKFSGSFDMPGFLMSEMKYKD
Homology
BLAST of Lcy01g004630 vs. ExPASy Swiss-Prot
Match: O23254 (Serine hydroxymethyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=SHM4 PE=1 SV=1)

HSP 1 Score: 906.0 bits (2340), Expect = 1.8e-262
Identity = 435/471 (92.36%), Postives = 453/471 (96.18%), Query Frame = 0

Query: 1   MDPVKAWGNTPLETVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+PV +WGNT L +VDPEI DLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MEPVSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EG+PGNRYYGGNEFIDEIENLCRSRALEAFH DPA WGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGIPGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKV+  TGYIDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKL+ICGGSAYPRDWDYARFRAIADK GALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPE AVYDFEDKINF+VFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEGAVYDFEDKINFAVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKTYAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQA +PGFK YAKQVKANAVALGNYLMSKGY++VT GTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLC+IT+NKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQI EFL RAVT+TL
Sbjct: 361 EKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIGEFLSRAVTLTL 420

Query: 421 SIQKEYGKLLKDFNKGLVNNKDIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
            IQK YGKLLKDFNKGLVNNKD+++LKADVEKFS S++MPGFLMSEMKYKD
Sbjct: 421 DIQKTYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMPGFLMSEMKYKD 471

BLAST of Lcy01g004630 vs. ExPASy Swiss-Prot
Match: Q9SVM4 (Serine hydroxymethyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=SHM5 PE=3 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 2.9e-236
Identity = 384/468 (82.05%), Postives = 430/468 (91.88%), Query Frame = 0

Query: 1   MDPVKAWGNTPLETVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+PV +WGNT L+ VDPEI+DLIEKEK RQCRGIELIA+ENFTS AV+EALGS LTNKYS
Sbjct: 1   MEPVYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGG EFIDEIE+LCRSR+LEAFH +P KWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF 180
           HDRIMGLDLPSGGH+THGYY+SGGK ISATSIYFE+LPYKVDS TGYIDYDKLEEKA+DF
Sbjct: 121 HDRIMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGG++YPR+WDYARFRA+ADK GA LLCDMAH S LVAAQEAA+PFEYCDVVTT
Sbjct: 181 RPKLIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           +THKSLRGPRAGMIFYRKGPKP KKGQPE  VYDF+ KIN +VFPALQ GPHN++IGALA
Sbjct: 241 STHKSLRGPRAGMIFYRKGPKPAKKGQPEGEVYDFDAKINSAVFPALQSGPHNNKIGALA 300

Query: 301 VALKQAMSPGFKTYAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQ M+P FK YAKQVKANA  L +YL++KGY LVT GT+NHL+LWDLRPLGLTGNKV
Sbjct: 301 VALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDGTDNHLILWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EK+C+LC IT+N+NAVFGD+S LAPGGVRIG PAMTSRGLVEKDFE+I EFLHRAVTITL
Sbjct: 361 EKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGLVEKDFEKIGEFLHRAVTITL 420

Query: 421 SIQKEYGKLLKDFNKGLVNNKDIEELKADVEKFSGSFDMPGFLMSEMK 469
            IQ++YGK++KDFNKGLVNNK+I+E+KADVE+F+  FDMPGF +SE +
Sbjct: 421 DIQEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMPGFFISESR 468

BLAST of Lcy01g004630 vs. ExPASy Swiss-Prot
Match: Q84WV0 (Serine hydroxymethyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=SHM7 PE=2 SV=1)

HSP 1 Score: 635.2 bits (1637), Expect = 5.9e-181
Identity = 296/466 (63.52%), Postives = 371/466 (79.61%), Query Frame = 0

Query: 4   VKAWGNTPLETVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGM 63
           V+AWG+ P+   DP+I +L+EKEK+RQ RGIELIASENF   AV+EALGS LTNKYSEGM
Sbjct: 130 VRAWGDQPIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALGSHLTNKYSEGM 189

Query: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQPHDR 123
           PG RYY GN++ID+IENLC  RAL AF ++  KWGVNVQPYS + ANFA YT LL P +R
Sbjct: 190 PGARYYTGNQYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTSANFAVYTGLLLPGER 249

Query: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDFRPK 183
           IMGLD PSGGH++HGY T GGKKISA SI+FES PYKV+  TGYIDYDKLE+KALD+RPK
Sbjct: 250 IMGLDSPSGGHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYIDYDKLEDKALDYRPK 309

Query: 184 LIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243
           ++ICGGS+YPRDWD+AR R IADKCGA+L+CDMAHISGLVA +E +NPF++CD+VT+TTH
Sbjct: 310 ILICGGSSYPRDWDFARVRQIADKCGAVLMCDMAHISGLVATKECSNPFDHCDIVTSTTH 369

Query: 244 KSLRGPRAGMIFYRKGPKPPKKGQ-----PEDAVYDFEDKINFSVFPALQGGPHNHQIGA 303
           K LRGPR G+IFYR+GPK  K+G           YD E+KINF+VFP+LQGGPHN+ I A
Sbjct: 370 KGLRGPRGGIIFYRRGPKIRKQGHHSSHCDTSTHYDLEEKINFAVFPSLQGGPHNNHIAA 429

Query: 304 LAVALKQAMSPGFKTYAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGN 363
           LA+ALKQ  +P +K Y +Q+K NA AL   L+ +  +LVTGGT+NHL+LWDL P+GLTG 
Sbjct: 430 LAIALKQVATPEYKAYIQQMKKNAQALAAALLRRKCRLVTGGTDNHLLLWDLTPMGLTGK 489

Query: 364 KVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTI 423
             EK+C++C+IT+NK A+FGD+  ++PGGVRIG PAMT+RG +E DFE +A+FL +A  I
Sbjct: 490 VYEKVCEMCHITLNKTAIFGDNGTISPGGVRIGTPAMTTRGCIESDFETMADFLIKAAQI 549

Query: 424 TLSIQKEYGKLLKDFNKGLVNNKDIEELKADVEKFSGSFDMPGFLM 465
           T ++Q+E+GK  K+F K L  NKDI EL+  VE F+  ++MP  L+
Sbjct: 550 TSALQREHGKSHKEFVKSLCTNKDIAELRNRVEAFALQYEMPASLI 595

BLAST of Lcy01g004630 vs. ExPASy Swiss-Prot
Match: Q9LM59 (Serine hydroxymethyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=SHM6 PE=2 SV=1)

HSP 1 Score: 630.9 bits (1626), Expect = 1.1e-179
Identity = 298/466 (63.95%), Postives = 372/466 (79.83%), Query Frame = 0

Query: 4   VKAWGNTPLETVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGM 63
           V+AWGN  +E  DPEI + +EKEK+RQ RGIELIASENF   AV+EALGS LTNKYSEGM
Sbjct: 134 VRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALGSHLTNKYSEGM 193

Query: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQPHDR 123
           PG RYY GN++ID+IE LC+ RAL AF ++  KWGVNVQPYS + ANFA +T LL P +R
Sbjct: 194 PGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFAVFTGLLMPGER 253

Query: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDFRPK 183
           IMGLD PSGGH++HGYYT GGKK+S  SI+FES PYKVD  TGYIDYDKLEEKALD+RPK
Sbjct: 254 IMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDYDKLEEKALDYRPK 313

Query: 184 LIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243
           ++ICGGS+YPRDW++ RFR IADKCGA+L+ DMA ISGLVAA+E+ NPF+YCD+VT+TTH
Sbjct: 314 ILICGGSSYPRDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPNPFDYCDIVTSTTH 373

Query: 244 KSLRGPRAGMIFYRKGPKPPKKG-----QPEDAVYDFEDKINFSVFPALQGGPHNHQIGA 303
           KSLRGPR G+IFY++G KP K+         +  YDFE+KINFSVFP+LQGGPHN+ I A
Sbjct: 374 KSLRGPRGGIIFYKRGLKPKKQSINLNHCESNIQYDFEEKINFSVFPSLQGGPHNNHIAA 433

Query: 304 LAVALKQAMSPGFKTYAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGN 363
           LA+ALKQA SP +K Y +QVK NA AL + L+S+  KL+TGGT+NHL+LWDL PLGLTG 
Sbjct: 434 LAIALKQAASPEYKLYMRQVKKNAKALASALISRKCKLITGGTDNHLLLWDLTPLGLTGK 493

Query: 364 KVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTI 423
             EK+C++C+ITVNK A+F ++  ++PGGVRIG+PAMTSRG +E +FE +A+FL+RA  I
Sbjct: 494 VYEKVCEMCHITVNKVAIFSENGVISPGGVRIGSPAMTSRGCLEPEFETMADFLYRAAQI 553

Query: 424 TLSIQKEYGKLLKDFNKGLVNNKDIEELKADVEKFSGSFDMPGFLM 465
             + Q+E+GKL K+  K + + K+I +L+  VE F+  F MP F M
Sbjct: 554 ASAAQREHGKLQKEPLKSIYHCKEIADLRNQVEAFATQFAMPAFDM 599

BLAST of Lcy01g004630 vs. ExPASy Swiss-Prot
Match: Q54Z26 (Serine hydroxymethyltransferase 1 OS=Dictyostelium discoideum OX=44689 GN=shmt1 PE=1 SV=1)

HSP 1 Score: 614.4 bits (1583), Expect = 1.1e-174
Identity = 300/454 (66.08%), Postives = 365/454 (80.40%), Query Frame = 0

Query: 8   GNTPLETVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNR 67
           GNTPL+ VD EIF+L+ +EK RQ +G+ELIASENFTS AV+EALGS  TNKY+EG PG+R
Sbjct: 6   GNTPLKEVDNEIFELMNREKDRQFKGLELIASENFTSRAVMEALGSHFTNKYAEGYPGSR 65

Query: 68  YYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQPHDRIMGL 127
           YYGG E +DE+E LC+ RAL+AF +D +KWGVNVQPYSGSPANFA YTALL+PHDRIMGL
Sbjct: 66  YYGGTEVVDELETLCQKRALKAFRLDESKWGVNVQPYSGSPANFAVYTALLRPHDRIMGL 125

Query: 128 DLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDFRPKLIIC 187
           DLPSGGHLTHGY T   KKISA+SI+FES+PY++  A G IDY +LEE AL F+PKLII 
Sbjct: 126 DLPSGGHLTHGYQTD-KKKISASSIFFESMPYQI-GADGLIDYQRLEENALLFKPKLIIS 185

Query: 188 GGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTHKSLR 247
           G SAYPR+WDY R RAIADK GA L+CDMAH SGLVAAQ   +PF+YCDVVT+TTHK+LR
Sbjct: 186 GASAYPREWDYKRMRAIADKVGAYLMCDMAHYSGLVAAQLLDSPFDYCDVVTSTTHKTLR 245

Query: 248 GPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALAVALKQAM 307
           GPR+G+IF+R+G +    G+ E   YD E KINF+VFP+LQGGPH + I  +AVALK+A 
Sbjct: 246 GPRSGIIFFRRGKRVDGNGK-EIEEYDIESKINFAVFPSLQGGPHENVIAGVAVALKEAD 305

Query: 308 SPGFKTYAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLC 367
           S  FK YA QVK NA A+GN LM+KGYKLVT GT+NHL+LWDLRP  LTGNK EK  D+ 
Sbjct: 306 SQEFKEYALQVKKNAAAIGNALMNKGYKLVTNGTDNHLILWDLRPKELTGNKFEKAADIA 365

Query: 368 NITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITLSIQKEYG 427
           NITVNKNAV GD++A++PGG+RIG+ A+TSRGL E DFE+IA+FL R V+I+L IQ   G
Sbjct: 366 NITVNKNAVHGDTNAISPGGIRIGSSALTSRGLKEADFEKIADFLDRIVSISLEIQGRVG 425

Query: 428 KLLKDFNKGLVNNKDIEELKADVEKFSGSFDMPG 462
           K L DF   +  +K++ +L+ +VE+FS  F +PG
Sbjct: 426 KKLVDFVVEINKSKELLDLRKEVEEFSSKFTLPG 456

BLAST of Lcy01g004630 vs. ExPASy TrEMBL
Match: A0A6J1CDF6 (Serine hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111009716 PE=3 SV=1)

HSP 1 Score: 954.5 bits (2466), Expect = 1.6e-274
Identity = 461/471 (97.88%), Postives = 465/471 (98.73%), Query Frame = 0

Query: 1   MDPVKAWGNTPLETVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+PV AWGNTPL+ VDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MEPVNAWGNTPLKAVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRALEAFH DPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHADPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKTYAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFK YAKQVKANAVALGNYLM+KGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 SIQKEYGKLLKDFNKGLVNNKDIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           +IQKEYGKLLKDFNKGLVNNKDIE LKADVEKFSGSFDMPGF MSEMKYKD
Sbjct: 421 NIQKEYGKLLKDFNKGLVNNKDIEGLKADVEKFSGSFDMPGFAMSEMKYKD 471

BLAST of Lcy01g004630 vs. ExPASy TrEMBL
Match: A0A6J1KLX2 (Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111494472 PE=3 SV=1)

HSP 1 Score: 952.6 bits (2461), Expect = 6.2e-274
Identity = 458/471 (97.24%), Postives = 467/471 (99.15%), Query Frame = 0

Query: 1   MDPVKAWGNTPLETVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MDPV AWGNTPLE VDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNAWGNTPLEAVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRAL+AFHVDPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKV+S+TGYIDY+KLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNSSTGYIDYEKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYA+FRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYAKFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKTYAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAM+PGFK YAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMAPGFKVYAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRA+TITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAITITL 420

Query: 421 SIQKEYGKLLKDFNKGLVNNKDIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           +IQKEYGKLLKDFNKGLVNNK+IEELKADVEKFS SFDMPGFLMSEMKYKD
Sbjct: 421 NIQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSASFDMPGFLMSEMKYKD 471

BLAST of Lcy01g004630 vs. ExPASy TrEMBL
Match: A0A0A0LKC8 (Serine hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G145880 PE=3 SV=1)

HSP 1 Score: 952.6 bits (2461), Expect = 6.2e-274
Identity = 458/471 (97.24%), Postives = 466/471 (98.94%), Query Frame = 0

Query: 1   MDPVKAWGNTPLETVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MDPV  WGNTPL TVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRAL+AFH DPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYD+EDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKTYAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAM+PGFK YAKQVKANAVALGNYLM+KGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMAPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 SIQKEYGKLLKDFNKGLVNNKDIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           ++QKEYGKLLKDFNKGLVNNK+IE+LKADVEKFSGSFDMPGFLMSEMKYKD
Sbjct: 421 NVQKEYGKLLKDFNKGLVNNKEIEKLKADVEKFSGSFDMPGFLMSEMKYKD 471

BLAST of Lcy01g004630 vs. ExPASy TrEMBL
Match: A0A6J1HIT2 (Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111464434 PE=3 SV=1)

HSP 1 Score: 952.6 bits (2461), Expect = 6.2e-274
Identity = 458/471 (97.24%), Postives = 467/471 (99.15%), Query Frame = 0

Query: 1   MDPVKAWGNTPLETVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MDPV AWGNTPLE VDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNAWGNTPLEAVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRAL+AFHVDPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKV+S+TGYIDY+KLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNSSTGYIDYEKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYA+FRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYAKFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKTYAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAM+PGFK YAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMAPGFKVYAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRA+TITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAITITL 420

Query: 421 SIQKEYGKLLKDFNKGLVNNKDIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           +IQKEYGKLLKDFNKGLVNNK+IEELKADVEKFS SFDMPGFLMSEMKYKD
Sbjct: 421 NIQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSASFDMPGFLMSEMKYKD 471

BLAST of Lcy01g004630 vs. ExPASy TrEMBL
Match: A0A6J1H4Y7 (Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111460192 PE=3 SV=1)

HSP 1 Score: 949.9 bits (2454), Expect = 4.0e-273
Identity = 458/471 (97.24%), Postives = 466/471 (98.94%), Query Frame = 0

Query: 1   MDPVKAWGNTPLETVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MDPV AWGNTPL  VDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNAWGNTPLVNVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATG+IDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGFIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPE+AVYDFEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPENAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKTYAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFK YAKQVKANAVALGNYLM+KGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKVYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 SIQKEYGKLLKDFNKGLVNNKDIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           ++QKE+GKLLKDFNKGLVNNK+IE LKADVEKFSGSF+MPGFLMSEMKYKD
Sbjct: 421 NVQKEHGKLLKDFNKGLVNNKEIEALKADVEKFSGSFEMPGFLMSEMKYKD 471

BLAST of Lcy01g004630 vs. NCBI nr
Match: XP_022138598.1 (serine hydroxymethyltransferase 4 [Momordica charantia])

HSP 1 Score: 954.5 bits (2466), Expect = 3.4e-274
Identity = 461/471 (97.88%), Postives = 465/471 (98.73%), Query Frame = 0

Query: 1   MDPVKAWGNTPLETVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+PV AWGNTPL+ VDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MEPVNAWGNTPLKAVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRALEAFH DPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHADPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKTYAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFK YAKQVKANAVALGNYLM+KGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 SIQKEYGKLLKDFNKGLVNNKDIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           +IQKEYGKLLKDFNKGLVNNKDIE LKADVEKFSGSFDMPGF MSEMKYKD
Sbjct: 421 NIQKEYGKLLKDFNKGLVNNKDIEGLKADVEKFSGSFDMPGFAMSEMKYKD 471

BLAST of Lcy01g004630 vs. NCBI nr
Match: XP_022964401.1 (serine hydroxymethyltransferase 4 [Cucurbita moschata] >XP_023000183.1 serine hydroxymethyltransferase 4 [Cucurbita maxima] >XP_023514660.1 serine hydroxymethyltransferase 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 952.6 bits (2461), Expect = 1.3e-273
Identity = 458/471 (97.24%), Postives = 467/471 (99.15%), Query Frame = 0

Query: 1   MDPVKAWGNTPLETVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MDPV AWGNTPLE VDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNAWGNTPLEAVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRAL+AFHVDPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKV+S+TGYIDY+KLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNSSTGYIDYEKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYA+FRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYAKFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKTYAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAM+PGFK YAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMAPGFKVYAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRA+TITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAITITL 420

Query: 421 SIQKEYGKLLKDFNKGLVNNKDIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           +IQKEYGKLLKDFNKGLVNNK+IEELKADVEKFS SFDMPGFLMSEMKYKD
Sbjct: 421 NIQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSASFDMPGFLMSEMKYKD 471

BLAST of Lcy01g004630 vs. NCBI nr
Match: XP_004138967.1 (serine hydroxymethyltransferase 4 [Cucumis sativus] >KGN61489.1 hypothetical protein Csa_006275 [Cucumis sativus])

HSP 1 Score: 952.6 bits (2461), Expect = 1.3e-273
Identity = 458/471 (97.24%), Postives = 466/471 (98.94%), Query Frame = 0

Query: 1   MDPVKAWGNTPLETVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MDPV  WGNTPL TVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNDWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRAL+AFH DPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHCDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYD+EDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDYEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKTYAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAM+PGFK YAKQVKANAVALGNYLM+KGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMAPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 SIQKEYGKLLKDFNKGLVNNKDIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           ++QKEYGKLLKDFNKGLVNNK+IE+LKADVEKFSGSFDMPGFLMSEMKYKD
Sbjct: 421 NVQKEYGKLLKDFNKGLVNNKEIEKLKADVEKFSGSFDMPGFLMSEMKYKD 471

BLAST of Lcy01g004630 vs. NCBI nr
Match: KAG6593753.1 (Serine hydroxymethyltransferase 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026085.1 Serine hydroxymethyltransferase 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 951.0 bits (2457), Expect = 3.7e-273
Identity = 457/471 (97.03%), Postives = 467/471 (99.15%), Query Frame = 0

Query: 1   MDPVKAWGNTPLETVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           MDPV AWGNTPLE VDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MDPVNAWGNTPLEAVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRAL+AFHVDPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKV+S+TGYIDY+KLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNSSTGYIDYEKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYA+FRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYAKFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKTYAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAM+PGFK YAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMAPGFKVYAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRA+TITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAITITL 420

Query: 421 SIQKEYGKLLKDFNKGLVNNKDIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           +IQKEYGKLLKDFNKGLVNNK+IEELKADVEKFS SFDMPGFLMS+MKYKD
Sbjct: 421 NIQKEYGKLLKDFNKGLVNNKEIEELKADVEKFSASFDMPGFLMSKMKYKD 471

BLAST of Lcy01g004630 vs. NCBI nr
Match: XP_038875387.1 (serine hydroxymethyltransferase 4 [Benincasa hispida])

HSP 1 Score: 951.0 bits (2457), Expect = 3.7e-273
Identity = 458/471 (97.24%), Postives = 465/471 (98.73%), Query Frame = 0

Query: 1   MDPVKAWGNTPLETVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+ V AWGNTPL TVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MESVNAWGNTPLNTVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGGNEFIDEIENLCRSRAL+AFHVDPAKWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGNEFIDEIENLCRSRALQAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDS TGYIDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSTTGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKTYAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQAMSPGFK YAKQVKANAVALGNYLM+KGYKLVTGGTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQAMSPGFKAYAKQVKANAVALGNYLMNKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLCNITVNKNAVFGDSSALAPGGVRIG PAMTSRGLVEKDFEQIAEFLHRAVTITL
Sbjct: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGTPAMTSRGLVEKDFEQIAEFLHRAVTITL 420

Query: 421 SIQKEYGKLLKDFNKGLVNNKDIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
           ++QKE+GKLLKDFNKGLVNNK+IEELKADVEKFSGSFDMPGFLMSEMKYKD
Sbjct: 421 NVQKEHGKLLKDFNKGLVNNKEIEELKADVEKFSGSFDMPGFLMSEMKYKD 471

BLAST of Lcy01g004630 vs. TAIR 10
Match: AT4G13930.1 (serine hydroxymethyltransferase 4 )

HSP 1 Score: 906.0 bits (2340), Expect = 1.3e-263
Identity = 435/471 (92.36%), Postives = 453/471 (96.18%), Query Frame = 0

Query: 1   MDPVKAWGNTPLETVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+PV +WGNT L +VDPEI DLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS
Sbjct: 1   MEPVSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EG+PGNRYYGGNEFIDEIENLCRSRALEAFH DPA WGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGIPGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF 180
           HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKV+  TGYIDYDKLEEKALDF
Sbjct: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKL+ICGGSAYPRDWDYARFRAIADK GALLLCDMAHISGLVAAQEAANPFEYCDVVTT
Sbjct: 181 RPKLLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           TTHKSLRGPRAGMIFYRKGPKPPKKGQPE AVYDFEDKINF+VFPALQGGPHNHQIGALA
Sbjct: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEGAVYDFEDKINFAVFPALQGGPHNHQIGALA 300

Query: 301 VALKQAMSPGFKTYAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQA +PGFK YAKQVKANAVALGNYLMSKGY++VT GTENHLVLWDLRPLGLTGNKV
Sbjct: 301 VALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EKLCDLC+IT+NKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQI EFL RAVT+TL
Sbjct: 361 EKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIGEFLSRAVTLTL 420

Query: 421 SIQKEYGKLLKDFNKGLVNNKDIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
            IQK YGKLLKDFNKGLVNNKD+++LKADVEKFS S++MPGFLMSEMKYKD
Sbjct: 421 DIQKTYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMPGFLMSEMKYKD 471

BLAST of Lcy01g004630 vs. TAIR 10
Match: AT4G13890.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 818.9 bits (2114), Expect = 2.1e-237
Identity = 384/468 (82.05%), Postives = 430/468 (91.88%), Query Frame = 0

Query: 1   MDPVKAWGNTPLETVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYS 60
           M+PV +WGNT L+ VDPEI+DLIEKEK RQCRGIELIA+ENFTS AV+EALGS LTNKYS
Sbjct: 1   MEPVYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYS 60

Query: 61  EGMPGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQP 120
           EGMPGNRYYGG EFIDEIE+LCRSR+LEAFH +P KWGVNVQPYSGSPANFAAYTALLQP
Sbjct: 61  EGMPGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQP 120

Query: 121 HDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDF 180
           HDRIMGLDLPSGGH+THGYY+SGGK ISATSIYFE+LPYKVDS TGYIDYDKLEEKA+DF
Sbjct: 121 HDRIMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDF 180

Query: 181 RPKLIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTT 240
           RPKLIICGG++YPR+WDYARFRA+ADK GA LLCDMAH S LVAAQEAA+PFEYCDVVTT
Sbjct: 181 RPKLIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTT 240

Query: 241 TTHKSLRGPRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALA 300
           +THKSLRGPRAGMIFYRKGPKP KKGQPE  VYDF+ KIN +VFPALQ GPHN++IGALA
Sbjct: 241 STHKSLRGPRAGMIFYRKGPKPAKKGQPEGEVYDFDAKINSAVFPALQSGPHNNKIGALA 300

Query: 301 VALKQAMSPGFKTYAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGNKV 360
           VALKQ M+P FK YAKQVKANA  L +YL++KGY LVT GT+NHL+LWDLRPLGLTGNKV
Sbjct: 301 VALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDGTDNHLILWDLRPLGLTGNKV 360

Query: 361 EKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITL 420
           EK+C+LC IT+N+NAVFGD+S LAPGGVRIG PAMTSRGLVEKDFE+I EFLHRAVTITL
Sbjct: 361 EKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGLVEKDFEKIGEFLHRAVTITL 420

Query: 421 SIQKEYGKLLKDFNKGLVNNKDIEELKADVEKFSGSFDMPGFLMSEMK 469
            IQ++YGK++KDFNKGLVNNK+I+E+KADVE+F+  FDMPGF +SE +
Sbjct: 421 DIQEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMPGFFISESR 468

BLAST of Lcy01g004630 vs. TAIR 10
Match: AT1G36370.1 (serine hydroxymethyltransferase 7 )

HSP 1 Score: 635.2 bits (1637), Expect = 4.2e-182
Identity = 296/466 (63.52%), Postives = 371/466 (79.61%), Query Frame = 0

Query: 4   VKAWGNTPLETVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGM 63
           V+AWG+ P+   DP+I +L+EKEK+RQ RGIELIASENF   AV+EALGS LTNKYSEGM
Sbjct: 130 VRAWGDQPIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALGSHLTNKYSEGM 189

Query: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQPHDR 123
           PG RYY GN++ID+IENLC  RAL AF ++  KWGVNVQPYS + ANFA YT LL P +R
Sbjct: 190 PGARYYTGNQYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTSANFAVYTGLLLPGER 249

Query: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDFRPK 183
           IMGLD PSGGH++HGY T GGKKISA SI+FES PYKV+  TGYIDYDKLE+KALD+RPK
Sbjct: 250 IMGLDSPSGGHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYIDYDKLEDKALDYRPK 309

Query: 184 LIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243
           ++ICGGS+YPRDWD+AR R IADKCGA+L+CDMAHISGLVA +E +NPF++CD+VT+TTH
Sbjct: 310 ILICGGSSYPRDWDFARVRQIADKCGAVLMCDMAHISGLVATKECSNPFDHCDIVTSTTH 369

Query: 244 KSLRGPRAGMIFYRKGPKPPKKGQ-----PEDAVYDFEDKINFSVFPALQGGPHNHQIGA 303
           K LRGPR G+IFYR+GPK  K+G           YD E+KINF+VFP+LQGGPHN+ I A
Sbjct: 370 KGLRGPRGGIIFYRRGPKIRKQGHHSSHCDTSTHYDLEEKINFAVFPSLQGGPHNNHIAA 429

Query: 304 LAVALKQAMSPGFKTYAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGN 363
           LA+ALKQ  +P +K Y +Q+K NA AL   L+ +  +LVTGGT+NHL+LWDL P+GLTG 
Sbjct: 430 LAIALKQVATPEYKAYIQQMKKNAQALAAALLRRKCRLVTGGTDNHLLLWDLTPMGLTGK 489

Query: 364 KVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTI 423
             EK+C++C+IT+NK A+FGD+  ++PGGVRIG PAMT+RG +E DFE +A+FL +A  I
Sbjct: 490 VYEKVCEMCHITLNKTAIFGDNGTISPGGVRIGTPAMTTRGCIESDFETMADFLIKAAQI 549

Query: 424 TLSIQKEYGKLLKDFNKGLVNNKDIEELKADVEKFSGSFDMPGFLM 465
           T ++Q+E+GK  K+F K L  NKDI EL+  VE F+  ++MP  L+
Sbjct: 550 TSALQREHGKSHKEFVKSLCTNKDIAELRNRVEAFALQYEMPASLI 595

BLAST of Lcy01g004630 vs. TAIR 10
Match: AT1G22020.1 (serine hydroxymethyltransferase 6 )

HSP 1 Score: 630.9 bits (1626), Expect = 8.0e-181
Identity = 298/466 (63.95%), Postives = 372/466 (79.83%), Query Frame = 0

Query: 4   VKAWGNTPLETVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGM 63
           V+AWGN  +E  DPEI + +EKEK+RQ RGIELIASENF   AV+EALGS LTNKYSEGM
Sbjct: 134 VRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALGSHLTNKYSEGM 193

Query: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQPHDR 123
           PG RYY GN++ID+IE LC+ RAL AF ++  KWGVNVQPYS + ANFA +T LL P +R
Sbjct: 194 PGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFAVFTGLLMPGER 253

Query: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDFRPK 183
           IMGLD PSGGH++HGYYT GGKK+S  SI+FES PYKVD  TGYIDYDKLEEKALD+RPK
Sbjct: 254 IMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDYDKLEEKALDYRPK 313

Query: 184 LIICGGSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243
           ++ICGGS+YPRDW++ RFR IADKCGA+L+ DMA ISGLVAA+E+ NPF+YCD+VT+TTH
Sbjct: 314 ILICGGSSYPRDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPNPFDYCDIVTSTTH 373

Query: 244 KSLRGPRAGMIFYRKGPKPPKKG-----QPEDAVYDFEDKINFSVFPALQGGPHNHQIGA 303
           KSLRGPR G+IFY++G KP K+         +  YDFE+KINFSVFP+LQGGPHN+ I A
Sbjct: 374 KSLRGPRGGIIFYKRGLKPKKQSINLNHCESNIQYDFEEKINFSVFPSLQGGPHNNHIAA 433

Query: 304 LAVALKQAMSPGFKTYAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGN 363
           LA+ALKQA SP +K Y +QVK NA AL + L+S+  KL+TGGT+NHL+LWDL PLGLTG 
Sbjct: 434 LAIALKQAASPEYKLYMRQVKKNAKALASALISRKCKLITGGTDNHLLLWDLTPLGLTGK 493

Query: 364 KVEKLCDLCNITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTI 423
             EK+C++C+ITVNK A+F ++  ++PGGVRIG+PAMTSRG +E +FE +A+FL+RA  I
Sbjct: 494 VYEKVCEMCHITVNKVAIFSENGVISPGGVRIGSPAMTSRGCLEPEFETMADFLYRAAQI 553

Query: 424 TLSIQKEYGKLLKDFNKGLVNNKDIEELKADVEKFSGSFDMPGFLM 465
             + Q+E+GKL K+  K + + K+I +L+  VE F+  F MP F M
Sbjct: 554 ASAAQREHGKLQKEPLKSIYHCKEIADLRNQVEAFATQFAMPAFDM 599

BLAST of Lcy01g004630 vs. TAIR 10
Match: AT4G37930.1 (serine transhydroxymethyltransferase 1 )

HSP 1 Score: 562.4 bits (1448), Expect = 3.5e-160
Identity = 277/468 (59.19%), Postives = 351/468 (75.00%), Query Frame = 0

Query: 9   NTPLETVDPEIFDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGMPGNRY 68
           N PLE VDPEI D+IE EK RQ +G+ELI SENFTS +V++A+GS +TNKYSEG PG RY
Sbjct: 52  NAPLEEVDPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARY 111

Query: 69  YGGNEFIDEIENLCRSRALEAFHVDPAKWGVNVQPYSGSPANFAAYTALLQPHDRIMGLD 128
           YGGNE+ID  E LC+ RALEAF +DP KWGVNVQP SGSPANF  YTALL+PH+RIM LD
Sbjct: 112 YGGNEYIDMAETLCQKRALEAFRLDPEKWGVNVQPLSGSPANFHVYTALLKPHERIMALD 171

Query: 129 LPSGGHLTHGYYTSGGKKISATSIYFESLPYKVDSATGYIDYDKLEEKALDFRPKLIICG 188
           LP GGHL+HGY T   KKISA SI+FE++PY++D +TGYIDYD++E+ A  FRPKLI+ G
Sbjct: 172 LPHGGHLSHGYQTD-TKKISAVSIFFETMPYRLDESTGYIDYDQMEKSATLFRPKLIVAG 231

Query: 189 GSAYPRDWDYARFRAIADKCGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTHKSLRG 248
            SAY R +DYAR R + +K  A++L DMAHISGLVAA    +PF+Y DVVTTTTHKSLRG
Sbjct: 232 ASAYARLYDYARIRKVCNKQKAVMLADMAHISGLVAANVIPSPFDYADVVTTTTHKSLRG 291

Query: 249 PRAGMIFYRKGPKPPKKGQPEDAVYDFEDKINFSVFPALQGGPHNHQIGALAVALKQAMS 308
           PR  MIF+RKG K   K Q ++ +YDFEDKIN +VFP LQGGPHNH I  LAVALKQA +
Sbjct: 292 PRGAMIFFRKGVKEINK-QGKEVLYDFEDKINQAVFPGLQGGPHNHTITGLAVALKQATT 351

Query: 309 PGFKTYAKQVKANAVALGNYLMSKGYKLVTGGTENHLVLWDLRPLGLTGNKVEKLCDLCN 368
             +K Y +QV +N+      LM +GY+LV+GGT+NHLVL +L+P G+ G++VEK+ +  +
Sbjct: 352 SEYKAYQEQVLSNSAKFAQTLMERGYELVSGGTDNHLVLVNLKPKGIDGSRVEKVLEAVH 411

Query: 369 ITVNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIAEFLHRAVTITLSIQKE-YG 428
           I  NKN V GD SA+ PGG+R+G PA+TSRG VE+DF ++AE+  +AVTI L ++ E  G
Sbjct: 412 IASNKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEYFDKAVTIALKVKSEAQG 471

Query: 429 KLLKDFNKGLVNN----KDIEELKADVEKFSGSFDMPGFLMSEMKYKD 472
             LKDF   + ++     +I +L+ +VE+F+  F   GF    MKYK+
Sbjct: 472 TKLKDFVSAMESSSTIQSEIAKLRHEVEEFAKQFPTIGFEKETMKYKN 517

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O232541.8e-26292.36Serine hydroxymethyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=SHM4 PE=1 S... [more]
Q9SVM42.9e-23682.05Serine hydroxymethyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=SHM5 PE=3 S... [more]
Q84WV05.9e-18163.52Serine hydroxymethyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=SHM7 PE=2 S... [more]
Q9LM591.1e-17963.95Serine hydroxymethyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=SHM6 PE=2 S... [more]
Q54Z261.1e-17466.08Serine hydroxymethyltransferase 1 OS=Dictyostelium discoideum OX=44689 GN=shmt1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1CDF61.6e-27497.88Serine hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111009716 P... [more]
A0A6J1KLX26.2e-27497.24Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111494472 PE=3... [more]
A0A0A0LKC86.2e-27497.24Serine hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G145880 PE=3 ... [more]
A0A6J1HIT26.2e-27497.24Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111464434 PE... [more]
A0A6J1H4Y74.0e-27397.24Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111460192 PE... [more]
Match NameE-valueIdentityDescription
XP_022138598.13.4e-27497.88serine hydroxymethyltransferase 4 [Momordica charantia][more]
XP_022964401.11.3e-27397.24serine hydroxymethyltransferase 4 [Cucurbita moschata] >XP_023000183.1 serine hy... [more]
XP_004138967.11.3e-27397.24serine hydroxymethyltransferase 4 [Cucumis sativus] >KGN61489.1 hypothetical pro... [more]
KAG6593753.13.7e-27397.03Serine hydroxymethyltransferase 4, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_038875387.13.7e-27397.24serine hydroxymethyltransferase 4 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT4G13930.11.3e-26392.36serine hydroxymethyltransferase 4 [more]
AT4G13890.12.1e-23782.05Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [more]
AT1G36370.14.2e-18263.52serine hydroxymethyltransferase 7 [more]
AT1G22020.18.0e-18163.95serine hydroxymethyltransferase 6 [more]
AT4G37930.13.5e-16059.19serine transhydroxymethyltransferase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR039429Serine hydroxymethyltransferase-like domainPFAMPF00464SHMTcoord: 12..411
e-value: 2.6E-192
score: 638.8
IPR015422Pyridoxal phosphate-dependent transferase, small domainGENE3D3.90.1150.10Aspartate Aminotransferase, domain 1coord: 16..460
e-value: 3.9E-215
score: 716.3
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3D3.40.640.10coord: 41..316
e-value: 3.9E-215
score: 716.3
IPR001085Serine hydroxymethyltransferasePIRSFPIRSF000412SHMTcoord: 1..466
e-value: 2.1E-189
score: 627.4
IPR001085Serine hydroxymethyltransferasePANTHERPTHR11680SERINE HYDROXYMETHYLTRANSFERASEcoord: 1..470
IPR001085Serine hydroxymethyltransferaseHAMAPMF_00051SHMTcoord: 11..461
score: 42.885849
IPR001085Serine hydroxymethyltransferaseCDDcd00378SHMTcoord: 13..453
e-value: 0.0
score: 619.218
NoneNo IPR availablePANTHERPTHR11680:SF33SERINE HYDROXYMETHYLTRANSFERASEcoord: 1..470
IPR019798Serine hydroxymethyltransferase, pyridoxal phosphate binding sitePROSITEPS00096SHMTcoord: 236..252
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 6..463

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy01g004630.1Lcy01g004630.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046655 folic acid metabolic process
biological_process GO:0019264 glycine biosynthetic process from serine
biological_process GO:0006565 L-serine catabolic process
biological_process GO:0032259 methylation
biological_process GO:0035999 tetrahydrofolate interconversion
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004372 glycine hydroxymethyltransferase activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0070905 serine binding
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0003824 catalytic activity