Lcy01g003740 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy01g003740
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial
Location	Chr01: 4502694 .. 4503380 (-)
RNA-Seq Expression	Lcy01g003740
Synteny	Lcy01g003740

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCATTCGCAGGATTGAGATGCATTAGGACTCAACCTTTGTTGCTTCACCCTGCTGCTTTGTTTACCAGAAGGAGGAGAGTTTTGTATTTTTCTGTGAAAGCCTCCCAATCCCAAACAGCTACTCAAGAAAAGCAAAGGCCTACCGCGATTTCACCACATTTTGATAAACAGATTATTGATAAAATCTCCAAGGACTATGAAGCAATTATTGGTATTGAAACCCATGTTCAGCTTTCCACTCTCACCAAGGCCTTCTGCAGCTGCCCTTACAATTATGGTTCTTCACCAAACACTAATATCTGCCCCATCTGTATGGGGCTTCCGGGTGCCTTACCTGTTTTGAACTCAAAAGTGATTGACTTTGCTGTGAAGTTGGGTCTTGCACTAAACTGCAGCTTGTCTTTCAACTCAAAGTTTGATAGGAAACAATACTTTTACCCAGACCTTCCCAAGGGCTATCAAATATCTCAGTTTGATGTCCCGATTGCGAGAGGCGGTTTTATTGATCTTGATCTTCCTATTGAGTATGGTGGGGGGCATAGGCGGTTCGGTATCACCAGGGTTCACATGGAAGAGGATGCAGGAAAGCTGCTTCACTCTGAAAGTGGAAGCTACTCTCAGGAAATAATTTTGCATCCCTTGTCTCATATTTTATATGATCCTCATTCCAAGCAAATATTTTAG

mRNA sequence

ATGGCATTCGCAGGATTGAGATGCATTAGGACTCAACCTTTGTTGCTTCACCCTGCTGCTTTGTTTACCAGAAGGAGGAGAGTTTTGTATTTTTCTGTGAAAGCCTCCCAATCCCAAACAGCTACTCAAGAAAAGCAAAGGCCTACCGCGATTTCACCACATTTTGATAAACAGATTATTGATAAAATCTCCAAGGACTATGAAGCAATTATTGGTATTGAAACCCATGTTCAGCTTTCCACTCTCACCAAGGCCTTCTGCAGCTGCCCTTACAATTATGGTTCTTCACCAAACACTAATATCTGCCCCATCTGTATGGGGCTTCCGGGTGCCTTACCTGTTTTGAACTCAAAAGTGATTGACTTTGCTGTGAAGTTGGGTCTTGCACTAAACTGCAGCTTGTCTTTCAACTCAAAGTTTGATAGGAAACAATACTTTTACCCAGACCTTCCCAAGGGCTATCAAATATCTCAGTTTGATGTCCCGATTGCGAGAGGCGGTTTTATTGATCTTGATCTTCCTATTGAGTATGGTGGGGGGCATAGGCGGTTCGGTATCACCAGGGTTCACATGGAAGAGGATGCAGGAAAGCTGCTTCACTCTGAAAGTGGAAGCTACTCTCAGGAAATAATTTTGCATCCCTTGTCTCATATTTTATATGATCCTCATTCCAAGCAAATATTTTAG

Coding sequence (CDS)

ATGGCATTCGCAGGATTGAGATGCATTAGGACTCAACCTTTGTTGCTTCACCCTGCTGCTTTGTTTACCAGAAGGAGGAGAGTTTTGTATTTTTCTGTGAAAGCCTCCCAATCCCAAACAGCTACTCAAGAAAAGCAAAGGCCTACCGCGATTTCACCACATTTTGATAAACAGATTATTGATAAAATCTCCAAGGACTATGAAGCAATTATTGGTATTGAAACCCATGTTCAGCTTTCCACTCTCACCAAGGCCTTCTGCAGCTGCCCTTACAATTATGGTTCTTCACCAAACACTAATATCTGCCCCATCTGTATGGGGCTTCCGGGTGCCTTACCTGTTTTGAACTCAAAAGTGATTGACTTTGCTGTGAAGTTGGGTCTTGCACTAAACTGCAGCTTGTCTTTCAACTCAAAGTTTGATAGGAAACAATACTTTTACCCAGACCTTCCCAAGGGCTATCAAATATCTCAGTTTGATGTCCCGATTGCGAGAGGCGGTTTTATTGATCTTGATCTTCCTATTGAGTATGGTGGGGGGCATAGGCGGTTCGGTATCACCAGGGTTCACATGGAAGAGGATGCAGGAAAGCTGCTTCACTCTGAAAGTGGAAGCTACTCTCAGGAAATAATTTTGCATCCCTTGTCTCATATTTTATATGATCCTCATTCCAAGCAAATATTTTAG

Protein sequence

MAFAGLRCIRTQPLLLHPAALFTRRRRVLYFSVKASQSQTATQEKQRPTAISPHFDKQIIDKISKDYEAIIGIETHVQLSTLTKAFCSCPYNYGSSPNTNICPICMGLPGALPVLNSKVIDFAVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDVPIARGGFIDLDLPIEYGGGHRRFGITRVHMEEDAGKLLHSESGSYSQEIILHPLSHILYDPHSKQIF

Homology

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BLAST of Lcy01g003740 vs. ExPASy Swiss-Prot
Match: B9SQR9 (Glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial OS=Ricinus communis OX=3988 GN=GATB PE=3 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 8.1e-83
Identity = 155/228 (67.98%), Postives = 179/228 (78.51%), Query Frame = 0


Query: 1   MAFAGLRCIRTQPLLLHPAALFTRRRRVLYFSVKASQSQTATQEKQR--PTAISPHFDKQ 60
           MA    R I+T PLLL+P + F R+  V Y +V  S S TATQEKQ+     +SP    +
Sbjct: 1   MASTLFRNIQTHPLLLYPTSFFRRKSSVFYCTVN-STSPTATQEKQQCPRIKVSPQTQSK 60

Query: 61  IIDKISKDYEAIIGIETHVQLSTLTKAFCSCPYNYGSSPNTNICPICMGLPGALPVLNSK 120
            +DK++KDYE IIGIETHVQLSTLTKAFCSCPYNYG+ PN+ ICP+CMGLPGALPVLNSK
Sbjct: 61  SLDKLTKDYETIIGIETHVQLSTLTKAFCSCPYNYGAQPNSTICPVCMGLPGALPVLNSK 120

Query: 121 VIDFAVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDVPIARGGFIDLDLPIEYG 180
           VI+FAVKLGLALNC LS NSKFDRKQYFYPDLPKGYQISQFD+PIA  G++DLDLP+E+G
Sbjct: 121 VIEFAVKLGLALNCKLSLNSKFDRKQYFYPDLPKGYQISQFDIPIATSGYVDLDLPVEFG 180

Query: 181 GGHRRFGITRVHMEEDAGKLLHSESGSYSQEIILH----PLSHILYDP 223
           GGHRRFGITRVHMEEDAGKLLHS  G     + L+    PL  I+ +P
Sbjct: 181 GGHRRFGITRVHMEEDAGKLLHSGGGISGAIVDLNRAGVPLLEIVSEP 227

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BLAST of Lcy01g003740 vs. ExPASy Swiss-Prot
Match: Q9FV81 (Glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=GATB PE=2 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 9.5e-76
Identity = 145/224 (64.73%), Postives = 174/224 (77.68%), Query Frame = 0


Query: 6   LRCIRTQPLLLHPAALFTRRRRVLYFSVKASQSQTATQEKQR---PTAISPHFDK-QIID 65
           LR I+     L   +L  RRRR   FSV++  SQT T  + +   PT ++P   K   +D
Sbjct: 6   LRTIQLNQFSLLGTSLL-RRRRSNNFSVRSCGSQTTTTHEAKQSSPTRVAPKNHKSNQLD 65

Query: 66  KISKDYEAIIGIETHVQLSTLTKAFCSCPYNYGSSPNTNICPICMGLPGALPVLNSKVID 125
           +I +DYEA+IGIETHVQLSTLTKAFCSC  NYGS PNT+ICP+CMGLPGALPVLNSKV++
Sbjct: 66  EILRDYEAVIGIETHVQLSTLTKAFCSCSNNYGSYPNTSICPVCMGLPGALPVLNSKVVE 125

Query: 126 FAVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDVPIARGGFIDLDLPIEYGGGH 185
           F V+LGLALNC LS  SKFDRKQYFYPDLPKGYQISQFD+PIA GG++D+D+P+E+GGGH
Sbjct: 126 FGVRLGLALNCDLSLKSKFDRKQYFYPDLPKGYQISQFDIPIASGGYVDVDIPLEFGGGH 185

Query: 186 RRFGITRVHMEEDAGKLLHSESGSYSQEIILH---PLSHILYDP 223
           RRFGITRVHMEEDAGKLLHS++G YSQ  +     PL  I+ +P
Sbjct: 186 RRFGITRVHMEEDAGKLLHSDTGDYSQVDLNRAGVPLLEIVSEP 228

[Close]

BLAST of Lcy01g003740 vs. ExPASy Swiss-Prot
Match: A2ZF53 (Glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial OS=Oryza sativa subsp. indica OX=39946 GN=GATB PE=3 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 5.1e-69
Identity = 130/225 (57.78%), Postives = 167/225 (74.22%), Query Frame = 0


Query: 1   MAFAGLRCIRTQPLLLHPAALFTRRRRVLYFSVKASQSQTATQEKQRPTAISPHFDKQII 60
           MA   LR +RT  +    A LF    +    S   +++++A     +   ++    K+  
Sbjct: 1   MALTLLRGMRTPVVARRNAGLFFTTLQTPLLSRFTTRAESARAAAHKSIQLA---TKEAA 60

Query: 61  DKISKDYEAIIGIETHVQLSTLTKAFCSCPYNYGSSPNTNICPICMGLPGALPVLNSKVI 120
           ++ ++ +EA+IGIETHVQLST+TKAFCSCPY+YGS PN+ +CP CMG PG LPVLN+KV+
Sbjct: 61  EQKTQSFEAVIGIETHVQLSTVTKAFCSCPYSYGSQPNSTVCPTCMGHPGTLPVLNAKVV 120

Query: 121 DFAVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDVPIARGGFIDLDLPIEYGGG 180
           + AVKLGLALNC ++  SKFDRKQYFYPDLPKGYQISQFD+PIA+ G++DLDLP+E+GGG
Sbjct: 121 ECAVKLGLALNCEIATTSKFDRKQYFYPDLPKGYQISQFDIPIAKEGYLDLDLPVEFGGG 180

Query: 181 HRRFGITRVHMEEDAGKLLHSESGSYSQEIILH---PLSHILYDP 223
           HRRFG+TRVHMEEDAGKLLHSESGSYSQ  +     PL  I+ +P
Sbjct: 181 HRRFGVTRVHMEEDAGKLLHSESGSYSQVDLNRAGVPLLEIVSEP 222

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BLAST of Lcy01g003740 vs. ExPASy Swiss-Prot
Match: C5Y3V8 (Glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial OS=Sorghum bicolor OX=4558 GN=GATB PE=3 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 1.9e-68
Identity = 136/228 (59.65%), Postives = 166/228 (72.81%), Query Frame = 0


Query: 1   MAFAGLRCIRTQPLLLHPAALF--TRRRRVLYFSVKASQSQTATQEKQRPTAISPHF-DK 60
           MA   LR +RT         LF    R R+  F+ +A  +Q AT+ K  P   S     K
Sbjct: 1   MALTLLRGMRTPVSSGSNPGLFFAVLRPRLSRFTARAESAQ-ATEPKAAPPPRSIQLATK 60

Query: 61  QIIDKISKDYEAIIGIETHVQLSTLTKAFCSCPYNYGSSPNTNICPICMGLPGALPVLNS 120
           +  ++ ++ +EA+IGIETHVQLST+TKAFCSCPY+YG+ PN+ +CP CMG PG LPVLN 
Sbjct: 61  EAAEQKTQGFEAVIGIETHVQLSTVTKAFCSCPYSYGAQPNSTVCPTCMGHPGTLPVLNE 120

Query: 121 KVIDFAVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDVPIARGGFIDLDLPIEY 180
           KV++ AVKLGLALNC +S  SKFDRKQYFYPDLPKGYQISQFD+PIA+ G +DLDLP+E+
Sbjct: 121 KVVECAVKLGLALNCEISMTSKFDRKQYFYPDLPKGYQISQFDIPIAKKGHVDLDLPVEF 180

Query: 181 GGGHRRFGITRVHMEEDAGKLLHSESGSYSQEIILH---PLSHILYDP 223
           GGGHR+FGITRVHMEEDAGKLLHSES SYSQ  +     PL  I+ +P
Sbjct: 181 GGGHRKFGITRVHMEEDAGKLLHSESSSYSQVDLNRAGVPLLEIVSEP 227

[Close]

BLAST of Lcy01g003740 vs. ExPASy Swiss-Prot
Match: Q2R2Z0 (Glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=GATB PE=2 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 2.5e-68
Identity = 133/227 (58.59%), Postives = 166/227 (73.13%), Query Frame = 0


Query: 1   MAFAGLRCIRTQPLLLHPAALF--TRRRRVLYFSVKASQSQTATQEKQRPTAISPHFDKQ 60
           MA   LR +RT  +    A LF  T +  +L      ++S  A   K    A      K+
Sbjct: 1   MALTLLRGMRTPVVARRNAGLFFTTLQSPLLSRFTMRAESARAAAPKSIQLA-----TKE 60

Query: 61  IIDKISKDYEAIIGIETHVQLSTLTKAFCSCPYNYGSSPNTNICPICMGLPGALPVLNSK 120
             ++ ++ +EA+IGIETHVQLST+TKAFCSCPY+YGS PN+ +CP CMG PG LPVLN+K
Sbjct: 61  AAEQKAQGFEAVIGIETHVQLSTVTKAFCSCPYSYGSQPNSTVCPTCMGHPGTLPVLNAK 120

Query: 121 VIDFAVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDVPIARGGFIDLDLPIEYG 180
           V++ AV+LGLALNC ++  SKFDRKQYFYPDLPKGYQISQFD+PIA+ G++DLDLP+E+G
Sbjct: 121 VVECAVRLGLALNCEIAMTSKFDRKQYFYPDLPKGYQISQFDIPIAKEGYLDLDLPVEFG 180

Query: 181 GGHRRFGITRVHMEEDAGKLLHSESGSYSQEIILH---PLSHILYDP 223
           GGHRRFG+TRVHMEEDAGKLLHSESGSYSQ  +     PL  I+ +P
Sbjct: 181 GGHRRFGVTRVHMEEDAGKLLHSESGSYSQVDLNRAGVPLLEIVSEP 222

[Close]

BLAST of Lcy01g003740 vs. ExPASy TrEMBL
Match: A0A6J1HL33 (Glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111464529 PE=3 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 7.3e-111
Identity = 203/225 (90.22%), Postives = 209/225 (92.89%), Query Frame = 0


Query: 1   MAFAGLRCIRTQPLLLHPAALFTRRRRVLYFSVKASQSQTATQEKQRPTAISPHFDKQII 60
           MAF GLRCIRTQP LLHPAALFTRRRRVLYFSVK+SQSQTATQEKQRP AISPHFDKQII
Sbjct: 1   MAFVGLRCIRTQPFLLHPAALFTRRRRVLYFSVKSSQSQTATQEKQRPIAISPHFDKQII 60

Query: 61  DKISKDYEAIIGIETHVQLSTLTKAFCSCPYNYGSSPNTNICPICMGLPGALPVLNSKVI 120
           DKISKDYEAIIGIETHVQLSTLTKAFCSCPYNYG SPNTNICPICMGLPGALPVLNSKVI
Sbjct: 61  DKISKDYEAIIGIETHVQLSTLTKAFCSCPYNYGVSPNTNICPICMGLPGALPVLNSKVI 120

Query: 121 DFAVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDVPIARGGFIDLDLPIEYGGG 180
           +FAVKLG ALNCSLSFNSKFDRKQYFYPDLPKGYQISQFD+PIA GGFIDLDLPIEYGGG
Sbjct: 121 EFAVKLGFALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDIPIASGGFIDLDLPIEYGGG 180

Query: 181 HRRFGITRVHMEEDAGKLLHSESGSYSQEIILH---PLSHILYDP 223
           HRRFGITRVHMEEDAGKLLHSESGSYSQ  +     PL  I+ +P
Sbjct: 181 HRRFGITRVHMEEDAGKLLHSESGSYSQVDLNRAGVPLLEIVSEP 225

[Close]

BLAST of Lcy01g003740 vs. ExPASy TrEMBL
Match: A0A6J1CA53 (Glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial OS=Momordica charantia OX=3673 GN=LOC111009788 PE=3 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 2.1e-110
Identity = 200/225 (88.89%), Postives = 212/225 (94.22%), Query Frame = 0


Query: 1   MAFAGLRCIRTQPLLLHPAALFTRRRRVLYFSVKASQSQTATQEKQRPTAISPHFDKQII 60
           MAFAGLRC RTQPLL++PAALF+RR RVLYFSVKASQSQTATQEKQRPTAISPHFDK++I
Sbjct: 1   MAFAGLRCFRTQPLLIYPAALFSRRNRVLYFSVKASQSQTATQEKQRPTAISPHFDKKVI 60

Query: 61  DKISKDYEAIIGIETHVQLSTLTKAFCSCPYNYGSSPNTNICPICMGLPGALPVLNSKVI 120
           DKISKDYEAIIGIETHVQLSTLTKAFCSCPYNYGSSPNTNICPICMGLPGALPVLNSKV+
Sbjct: 61  DKISKDYEAIIGIETHVQLSTLTKAFCSCPYNYGSSPNTNICPICMGLPGALPVLNSKVV 120

Query: 121 DFAVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDVPIARGGFIDLDLPIEYGGG 180
           DFAVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDVPIA GG+IDLD+PIE+GGG
Sbjct: 121 DFAVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDVPIASGGYIDLDIPIEFGGG 180

Query: 181 HRRFGITRVHMEEDAGKLLHSESGSYSQEIILH---PLSHILYDP 223
           HRRFGITRVHMEEDAGKLLHSESGSYSQ  +     PL  I+ +P
Sbjct: 181 HRRFGITRVHMEEDAGKLLHSESGSYSQVDLNRAGVPLLEIVSEP 225

[Close]

BLAST of Lcy01g003740 vs. ExPASy TrEMBL
Match: A0A6J1KDS0 (Glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111494743 PE=3 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 2.8e-110
Identity = 201/225 (89.33%), Postives = 210/225 (93.33%), Query Frame = 0


Query: 1   MAFAGLRCIRTQPLLLHPAALFTRRRRVLYFSVKASQSQTATQEKQRPTAISPHFDKQII 60
           MAF GLRCIRTQP LLHPAALFT+RRRVLYFSVK+SQSQTATQEKQRP AISPHFDKQII
Sbjct: 1   MAFVGLRCIRTQPFLLHPAALFTKRRRVLYFSVKSSQSQTATQEKQRPIAISPHFDKQII 60

Query: 61  DKISKDYEAIIGIETHVQLSTLTKAFCSCPYNYGSSPNTNICPICMGLPGALPVLNSKVI 120
           D+ISKDYEAIIGIETHVQLSTLTKAFCSCPYNYG SPNTNICPICMGLPGALPVLN+KVI
Sbjct: 61  DRISKDYEAIIGIETHVQLSTLTKAFCSCPYNYGVSPNTNICPICMGLPGALPVLNTKVI 120

Query: 121 DFAVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDVPIARGGFIDLDLPIEYGGG 180
           +FAVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFD+PIA GGFIDLDLPIEYGGG
Sbjct: 121 EFAVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDIPIASGGFIDLDLPIEYGGG 180

Query: 181 HRRFGITRVHMEEDAGKLLHSESGSYSQEIILH---PLSHILYDP 223
           HRRFGITRVHMEEDAGKLLHSESGSYSQ  +     PL  I+ +P
Sbjct: 181 HRRFGITRVHMEEDAGKLLHSESGSYSQVDLNRAGVPLLEIVSEP 225

[Close]

BLAST of Lcy01g003740 vs. ExPASy TrEMBL
Match: A0A0A0LIG1 (Glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial OS=Cucumis sativus OX=3659 GN=GATB PE=3 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 8.0e-110
Identity = 197/210 (93.81%), Postives = 204/210 (97.14%), Query Frame = 0


Query: 1   MAFAGLRCIRTQPLLLHPAALFTRRRRVLYFSVKASQSQTATQEKQRPTAISPHFDKQII 60
           MAF GLRCIRT PLL HPAALF+RR++VLYFSVKASQSQTATQEKQRP AISPHFDKQII
Sbjct: 1   MAFVGLRCIRTLPLLFHPAALFSRRKKVLYFSVKASQSQTATQEKQRPMAISPHFDKQII 60

Query: 61  DKISKDYEAIIGIETHVQLSTLTKAFCSCPYNYGSSPNTNICPICMGLPGALPVLNSKVI 120
           DKISKDYEA+IGIETHVQLSTLTKAFCSCPYNYGSSPNTNICPICMGLPGALPVLNSKVI
Sbjct: 61  DKISKDYEAVIGIETHVQLSTLTKAFCSCPYNYGSSPNTNICPICMGLPGALPVLNSKVI 120

Query: 121 DFAVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDVPIARGGFIDLDLPIEYGGG 180
           + AVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDVPIA GG+IDLDLPIEYGGG
Sbjct: 121 ESAVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDVPIASGGYIDLDLPIEYGGG 180

Query: 181 HRRFGITRVHMEEDAGKLLHSESGSYSQEI 211
           HRRFGITRVHMEEDAGKLLHSESGSYSQE+
Sbjct: 181 HRRFGITRVHMEEDAGKLLHSESGSYSQEM 210

[Close]

BLAST of Lcy01g003740 vs. ExPASy TrEMBL
Match: A0A6J1H4Z2 (Glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111460195 PE=3 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 2.6e-108
Identity = 198/225 (88.00%), Postives = 210/225 (93.33%), Query Frame = 0


Query: 1   MAFAGLRCIRTQPLLLHPAALFTRRRRVLYFSVKASQSQTATQEKQRPTAISPHFDKQII 60
           MAFA LRCIR+Q LLLHPAALFT+RRRVLYFSVKASQSQTATQEK+RPTAISPHFDKQII
Sbjct: 1   MAFAALRCIRSQSLLLHPAALFTQRRRVLYFSVKASQSQTATQEKKRPTAISPHFDKQII 60

Query: 61  DKISKDYEAIIGIETHVQLSTLTKAFCSCPYNYGSSPNTNICPICMGLPGALPVLNSKVI 120
           D+ISK+YEAIIGIETHVQLST TKAFCSCPYNYGSSPNTNICPICMGLPGALPVLNSKVI
Sbjct: 61  DQISKNYEAIIGIETHVQLSTFTKAFCSCPYNYGSSPNTNICPICMGLPGALPVLNSKVI 120

Query: 121 DFAVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDVPIARGGFIDLDLPIEYGGG 180
           +FAVKLGLALNC LSFNSKFDRKQYFYPDLPKGYQISQFD+PIA GG+IDLDLP+EYGGG
Sbjct: 121 EFAVKLGLALNCRLSFNSKFDRKQYFYPDLPKGYQISQFDIPIASGGYIDLDLPLEYGGG 180

Query: 181 HRRFGITRVHMEEDAGKLLHSESGSYSQEIILH---PLSHILYDP 223
           HRRFGITRVHMEEDAGKLLHSESGSYSQ  +     PL  I+ +P
Sbjct: 181 HRRFGITRVHMEEDAGKLLHSESGSYSQVDLNRAGVPLLEIVSEP 225

[Close]

BLAST of Lcy01g003740 vs. NCBI nr
Match: KAG6593809.1 (Glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 432.6 bits (1111), Expect = 2.2e-117
Identity = 211/219 (96.35%), Postives = 214/219 (97.72%), Query Frame = 0


Query: 1   MAFAGLRCIRTQPLLLHPAALFTRRRRVLYFSVKASQSQTATQEKQRPTAISPHFDKQII 60
           MAF GLRCIRTQP LLHPAALFTRRRRVLYFSVK+SQSQTATQEKQRP AISPHFDKQII
Sbjct: 1   MAFVGLRCIRTQPFLLHPAALFTRRRRVLYFSVKSSQSQTATQEKQRPIAISPHFDKQII 60

Query: 61  DKISKDYEAIIGIETHVQLSTLTKAFCSCPYNYGSSPNTNICPICMGLPGALPVLNSKVI 120
           DKISKDYEAIIGIETHVQLSTLTKAFCSCPYNYG SPNTNICPICMGLPGALPVLNSKVI
Sbjct: 61  DKISKDYEAIIGIETHVQLSTLTKAFCSCPYNYGVSPNTNICPICMGLPGALPVLNSKVI 120

Query: 121 DFAVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDVPIARGGFIDLDLPIEYGGG 180
           +FAVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFD+PIA GGFIDLDLPIEYGGG
Sbjct: 121 EFAVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDIPIASGGFIDLDLPIEYGGG 180

Query: 181 HRRFGITRVHMEEDAGKLLHSESGSYSQEIILHPLSHIL 220
           HRRFGITRVHMEEDAGKLLHSESGSYSQEIILHPLSHIL
Sbjct: 181 HRRFGITRVHMEEDAGKLLHSESGSYSQEIILHPLSHIL 219

[Close]

BLAST of Lcy01g003740 vs. NCBI nr
Match: KAG7026143.1 (Glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 432.6 bits (1111), Expect = 2.2e-117
Identity = 211/219 (96.35%), Postives = 214/219 (97.72%), Query Frame = 0


Query: 1   MAFAGLRCIRTQPLLLHPAALFTRRRRVLYFSVKASQSQTATQEKQRPTAISPHFDKQII 60
           MAF GLRCIRTQP LLHPAALFTRRRRVLYFSVK+SQSQTATQEKQRP AISPHFDKQII
Sbjct: 1   MAFVGLRCIRTQPFLLHPAALFTRRRRVLYFSVKSSQSQTATQEKQRPIAISPHFDKQII 60

Query: 61  DKISKDYEAIIGIETHVQLSTLTKAFCSCPYNYGSSPNTNICPICMGLPGALPVLNSKVI 120
           DKISKDYEAIIGIETHVQLSTLTKAFCSCPYNYG SPNTNICPICMGLPGALPVLNSKVI
Sbjct: 61  DKISKDYEAIIGIETHVQLSTLTKAFCSCPYNYGVSPNTNICPICMGLPGALPVLNSKVI 120

Query: 121 DFAVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDVPIARGGFIDLDLPIEYGGG 180
           +FAVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFD+PIA GGFIDLDLPIEYGGG
Sbjct: 121 EFAVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDIPIASGGFIDLDLPIEYGGG 180

Query: 181 HRRFGITRVHMEEDAGKLLHSESGSYSQEIILHPLSHIL 220
           HRRFGITRVHMEEDAGKLLHSESGSYSQEIILHPLSHIL
Sbjct: 181 HRRFGITRVHMEEDAGKLLHSESGSYSQEIILHPLSHIL 219

[Close]

BLAST of Lcy01g003740 vs. NCBI nr
Match: XP_038876097.1 (glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial [Benincasa hispida] >XP_038876098.1 glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial [Benincasa hispida])

HSP 1 Score: 411.4 bits (1056), Expect = 5.2e-111
Identity = 204/225 (90.67%), Postives = 212/225 (94.22%), Query Frame = 0


Query: 1   MAFAGLRCIRTQPLLLHPAALFTRRRRVLYFSVKASQSQTATQEKQRPTAISPHFDKQII 60
           MAFAG+RCIRTQPLL HPAALFTRR+RVLYFSVKASQ+QTATQEKQRPTAISPHFDKQII
Sbjct: 1   MAFAGVRCIRTQPLLFHPAALFTRRKRVLYFSVKASQAQTATQEKQRPTAISPHFDKQII 60

Query: 61  DKISKDYEAIIGIETHVQLSTLTKAFCSCPYNYGSSPNTNICPICMGLPGALPVLNSKVI 120
           DKISKDYEAIIGIETHVQLSTLTKAFCSCPYNYGSSPNTNICPICMGLPGALPVLNSKVI
Sbjct: 61  DKISKDYEAIIGIETHVQLSTLTKAFCSCPYNYGSSPNTNICPICMGLPGALPVLNSKVI 120

Query: 121 DFAVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDVPIARGGFIDLDLPIEYGGG 180
           + AVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDVPIA GG+IDLDLPIEYGGG
Sbjct: 121 ESAVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDVPIASGGYIDLDLPIEYGGG 180

Query: 181 HRRFGITRVHMEEDAGKLLHSESGSYSQEIILH---PLSHILYDP 223
           HRRFGITRVHMEEDAGKLLHSESGSYSQ  +     PL  I+ +P
Sbjct: 181 HRRFGITRVHMEEDAGKLLHSESGSYSQVDLNRAGVPLLEIVSEP 225

[Close]

BLAST of Lcy01g003740 vs. NCBI nr
Match: KAE8651796.1 (hypothetical protein Csa_005907 [Cucumis sativus])

HSP 1 Score: 411.0 bits (1055), Expect = 6.7e-111
Identity = 199/214 (92.99%), Postives = 207/214 (96.73%), Query Frame = 0


Query: 1   MAFAGLRCIRTQPLLLHPAALFTRRRRVLYFSVKASQSQTATQEKQRPTAISPHFDKQII 60
           MAF GLRCIRT PLL HPAALF+RR++VLYFSVKASQSQTATQEKQRP AISPHFDKQII
Sbjct: 1   MAFVGLRCIRTLPLLFHPAALFSRRKKVLYFSVKASQSQTATQEKQRPMAISPHFDKQII 60

Query: 61  DKISKDYEAIIGIETHVQLSTLTKAFCSCPYNYGSSPNTNICPICMGLPGALPVLNSKVI 120
           DKISKDYEA+IGIETHVQLSTLTKAFCSCPYNYGSSPNTNICPICMGLPGALPVLNSKVI
Sbjct: 61  DKISKDYEAVIGIETHVQLSTLTKAFCSCPYNYGSSPNTNICPICMGLPGALPVLNSKVI 120

Query: 121 DFAVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDVPIARGGFIDLDLPIEYGGG 180
           + AVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDVPIA GG+IDLDLPIEYGGG
Sbjct: 121 ESAVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDVPIASGGYIDLDLPIEYGGG 180

Query: 181 HRRFGITRVHMEEDAGKLLHSESGSYSQEIILHP 215
           HRRFGITRVHMEEDAGKLLHSESGSYSQE+ L+P
Sbjct: 181 HRRFGITRVHMEEDAGKLLHSESGSYSQEMTLYP 214

[Close]

BLAST of Lcy01g003740 vs. NCBI nr
Match: XP_022964528.1 (glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial-like [Cucurbita moschata] >XP_022964529.1 glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 409.8 bits (1052), Expect = 1.5e-110
Identity = 203/225 (90.22%), Postives = 209/225 (92.89%), Query Frame = 0


Query: 1   MAFAGLRCIRTQPLLLHPAALFTRRRRVLYFSVKASQSQTATQEKQRPTAISPHFDKQII 60
           MAF GLRCIRTQP LLHPAALFTRRRRVLYFSVK+SQSQTATQEKQRP AISPHFDKQII
Sbjct: 1   MAFVGLRCIRTQPFLLHPAALFTRRRRVLYFSVKSSQSQTATQEKQRPIAISPHFDKQII 60

Query: 61  DKISKDYEAIIGIETHVQLSTLTKAFCSCPYNYGSSPNTNICPICMGLPGALPVLNSKVI 120
           DKISKDYEAIIGIETHVQLSTLTKAFCSCPYNYG SPNTNICPICMGLPGALPVLNSKVI
Sbjct: 61  DKISKDYEAIIGIETHVQLSTLTKAFCSCPYNYGVSPNTNICPICMGLPGALPVLNSKVI 120

Query: 121 DFAVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDVPIARGGFIDLDLPIEYGGG 180
           +FAVKLG ALNCSLSFNSKFDRKQYFYPDLPKGYQISQFD+PIA GGFIDLDLPIEYGGG
Sbjct: 121 EFAVKLGFALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDIPIASGGFIDLDLPIEYGGG 180

Query: 181 HRRFGITRVHMEEDAGKLLHSESGSYSQEIILH---PLSHILYDP 223
           HRRFGITRVHMEEDAGKLLHSESGSYSQ  +     PL  I+ +P
Sbjct: 181 HRRFGITRVHMEEDAGKLLHSESGSYSQVDLNRAGVPLLEIVSEP 225

[Close]

BLAST of Lcy01g003740 vs. TAIR 10
Match: AT1G48520.2 (GLU-ADT subunit B )

HSP 1 Score: 284.6 bits (727), Expect = 6.8e-77
Identity = 145/224 (64.73%), Postives = 174/224 (77.68%), Query Frame = 0


Query: 6   LRCIRTQPLLLHPAALFTRRRRVLYFSVKASQSQTATQEKQR---PTAISPHFDK-QIID 65
           LR I+     L   +L  RRRR   FSV++  SQT T  + +   PT ++P   K   +D
Sbjct: 6   LRTIQLNQFSLLGTSLL-RRRRSNNFSVRSCGSQTTTTHEAKQSSPTRVAPKNHKSNQLD 65

Query: 66  KISKDYEAIIGIETHVQLSTLTKAFCSCPYNYGSSPNTNICPICMGLPGALPVLNSKVID 125
           +I +DYEA+IGIETHVQLSTLTKAFCSC  NYGS PNT+ICP+CMGLPGALPVLNSKV++
Sbjct: 66  EILRDYEAVIGIETHVQLSTLTKAFCSCSNNYGSYPNTSICPVCMGLPGALPVLNSKVVE 125

Query: 126 FAVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDVPIARGGFIDLDLPIEYGGGH 185
           F V+LGLALNC LS  SKFDRKQYFYPDLPKGYQISQFD+PIA GG++D+D+P+E+GGGH
Sbjct: 126 FGVRLGLALNCDLSLKSKFDRKQYFYPDLPKGYQISQFDIPIASGGYVDVDIPLEFGGGH 185

Query: 186 RRFGITRVHMEEDAGKLLHSESGSYSQEIILH---PLSHILYDP 223
           RRFGITRVHMEEDAGKLLHS++G YSQ  +     PL  I+ +P
Sbjct: 186 RRFGITRVHMEEDAGKLLHSDTGDYSQVDLNRAGVPLLEIVSEP 228

[Close]

BLAST of Lcy01g003740 vs. TAIR 10
Match: AT1G48520.3 (GLU-ADT subunit B )

HSP 1 Score: 284.6 bits (727), Expect = 6.8e-77
Identity = 145/224 (64.73%), Postives = 174/224 (77.68%), Query Frame = 0


Query: 6   LRCIRTQPLLLHPAALFTRRRRVLYFSVKASQSQTATQEKQR---PTAISPHFDK-QIID 65
           LR I+     L   +L  RRRR   FSV++  SQT T  + +   PT ++P   K   +D
Sbjct: 6   LRTIQLNQFSLLGTSLL-RRRRSNNFSVRSCGSQTTTTHEAKQSSPTRVAPKNHKSNQLD 65

Query: 66  KISKDYEAIIGIETHVQLSTLTKAFCSCPYNYGSSPNTNICPICMGLPGALPVLNSKVID 125
           +I +DYEA+IGIETHVQLSTLTKAFCSC  NYGS PNT+ICP+CMGLPGALPVLNSKV++
Sbjct: 66  EILRDYEAVIGIETHVQLSTLTKAFCSCSNNYGSYPNTSICPVCMGLPGALPVLNSKVVE 125

Query: 126 FAVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDVPIARGGFIDLDLPIEYGGGH 185
           F V+LGLALNC LS  SKFDRKQYFYPDLPKGYQISQFD+PIA GG++D+D+P+E+GGGH
Sbjct: 126 FGVRLGLALNCDLSLKSKFDRKQYFYPDLPKGYQISQFDIPIASGGYVDVDIPLEFGGGH 185

Query: 186 RRFGITRVHMEEDAGKLLHSESGSYSQEIILH---PLSHILYDP 223
           RRFGITRVHMEEDAGKLLHS++G YSQ  +     PL  I+ +P
Sbjct: 186 RRFGITRVHMEEDAGKLLHSDTGDYSQVDLNRAGVPLLEIVSEP 228

[Close]

BLAST of Lcy01g003740 vs. TAIR 10
Match: AT1G48520.1 (GLU-ADT subunit B )

HSP 1 Score: 284.6 bits (727), Expect = 6.8e-77
Identity = 145/224 (64.73%), Postives = 174/224 (77.68%), Query Frame = 0


Query: 6   LRCIRTQPLLLHPAALFTRRRRVLYFSVKASQSQTATQEKQR---PTAISPHFDK-QIID 65
           LR I+     L   +L  RRRR   FSV++  SQT T  + +   PT ++P   K   +D
Sbjct: 6   LRTIQLNQFSLLGTSLL-RRRRSNNFSVRSCGSQTTTTHEAKQSSPTRVAPKNHKSNQLD 65

Query: 66  KISKDYEAIIGIETHVQLSTLTKAFCSCPYNYGSSPNTNICPICMGLPGALPVLNSKVID 125
           +I +DYEA+IGIETHVQLSTLTKAFCSC  NYGS PNT+ICP+CMGLPGALPVLNSKV++
Sbjct: 66  EILRDYEAVIGIETHVQLSTLTKAFCSCSNNYGSYPNTSICPVCMGLPGALPVLNSKVVE 125

Query: 126 FAVKLGLALNCSLSFNSKFDRKQYFYPDLPKGYQISQFDVPIARGGFIDLDLPIEYGGGH 185
           F V+LGLALNC LS  SKFDRKQYFYPDLPKGYQISQFD+PIA GG++D+D+P+E+GGGH
Sbjct: 126 FGVRLGLALNCDLSLKSKFDRKQYFYPDLPKGYQISQFDIPIASGGYVDVDIPLEFGGGH 185

Query: 186 RRFGITRVHMEEDAGKLLHSESGSYSQEIILH---PLSHILYDP 223
           RRFGITRVHMEEDAGKLLHS++G YSQ  +     PL  I+ +P
Sbjct: 186 RRFGITRVHMEEDAGKLLHSDTGDYSQVDLNRAGVPLLEIVSEP 228

The following BLAST results are available for this feature:

BLAST of Lcy01g003740 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B9SQR9	8.1e-83	67.98	Glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial OS=Ri...	[more]
Q9FV81	9.5e-76	64.73	Glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial OS=Ar...	[more]
A2ZF53	5.1e-69	57.78	Glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial OS=Or...	[more]
C5Y3V8	1.9e-68	59.65	Glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial OS=So...	[more]
Q2R2Z0	2.5e-68	58.59	Glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial OS=Or...	[more]

BLAST of Lcy01g003740 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HL33	7.3e-111	90.22	Glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial OS=Cu...	[more]
A0A6J1CA53	2.1e-110	88.89	Glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial OS=Mo...	[more]
A0A6J1KDS0	2.8e-110	89.33	Glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial OS=Cu...	[more]
A0A0A0LIG1	8.0e-110	93.81	Glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial OS=Cu...	[more]
A0A6J1H4Z2	2.6e-108	88.00	Glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial OS=Cu...	[more]

BLAST of Lcy01g003740 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6593809.1	2.2e-117	96.35	Glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial, part...	[more]
KAG7026143.1	2.2e-117	96.35	Glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial, part...	[more]
XP_038876097.1	5.2e-111	90.67	glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial [Beni...	[more]
KAE8651796.1	6.7e-111	92.99	hypothetical protein Csa_005907 [Cucumis sativus]	[more]
XP_022964528.1	1.5e-110	90.22	glutamyl-tRNA(Gln) amidotransferase subunit B, chloroplastic/mitochondrial-like ...	[more]

BLAST of Lcy01g003740 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G48520.2	6.8e-77	64.73	GLU-ADT subunit B	[more]
AT1G48520.3	6.8e-77	64.73	GLU-ADT subunit B	[more]
AT1G48520.1	6.8e-77	64.73	GLU-ADT subunit B	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006075	Aspartyl/Glutamyl-tRNA(Gln) amidotransferase, subunit B/E, catalytic	PFAM	PF02934	GatB_N	coord: 70..203 e-value: 4.6E-48 score: 163.9
None	No IPR available	PANTHER	PTHR11659:SF0	GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT B, MITOCHONDRIAL	coord: 57..208
IPR017959	Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, subunit B /E	PANTHER	PTHR11659	GLUTAMYL-TRNA GLN AMIDOTRANSFERASE SUBUNIT B MITOCHONDRIAL AND PROKARYOTIC PET112-RELATED	coord: 57..208
IPR014746	Glutamine synthetase/guanido kinase, catalytic domain	SUPERFAMILY	55931	Glutamine synthetase/guanido kinase	coord: 68..206

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy01g003740.1	Lcy01g003740.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0070681	glutaminyl-tRNAGln biosynthesis via transamidation
biological_process	GO:0032543	mitochondrial translation
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0030956	glutamyl-tRNA(Gln) amidotransferase complex
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0050567	glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity
molecular_function	GO:0016740	transferase activity
molecular_function	GO:0016884	carbon-nitrogen ligase activity, with glutamine as amido-N-donor
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0016874	ligase activity