Lcy01g003380 (gene) Sponge gourd (P93075) v1

Overview
NameLcy01g003380
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description11S globulin subunit beta
LocationChr01: 4066927 .. 4069789 (+)
RNA-Seq ExpressionLcy01g003380
SyntenyLcy01g003380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGATTCCTACATACCGATATGTAGCAGTGGAAAAATTTCTAGTACTACGATGGAAAATTTCATCCATTGTTTATCATGTAAGAACTATTTAAAAATGGTGTGCGACCCATGAAATAAAATATTATGTTAGTAATGCTCATTAAATGATGTGTCAAATGCTGAATGGATAGAGTAAATACTACAGAAAGTTTTTTCCTTGTATTTTTCTCTAACATTATTCTCTCTCCACTCCTTCTTCTTCATTCTTTCATCTCTCCCCTCCTACTTTGTGCCTTCTCTTCTCCATTTGTTGTCTCTTATTTGAAAAAAAAAAAAAAACCAATGTAAAAGAAAAACATGCAAAATTTACCAAGTTATAAATCACGTACCTTTAAAGTGGACAAAGATTGAGCTGGATGACCCTGAAGAACAACGAGTCAGAACCGCCATGGCAACTGGTGCGTCTCTTCATGAATCCCTCCACCACGTATTCTCTTACCGCCACCTCCATCCATTTGTCACCGCCATTCCTCCTCCGGCCACCTATAAATACTCCTCCGCCTTCTTCTCCGTTCCTCACATTCACAACAATTACCGATCAGCCTTCTCTTCTCCATACTCATGGCTCGCCCTTCTTTTCTTTCCTTCTTATGTCTAGCAGTTTTCATCCATGCCTGCCTCGCTCAGATTGAGCAGATCCCTTGGGAGTTCCAAGGCGGCGAAATGAGGCAACAGCACCGCTTCCAATCTTCCAGAGCCTGTCGTCTCGAGAATCTCCGAGCTCACGAGCCCGTTCGCAGGGCGGAGGCGGAGGCCGGTTTCACCGAAGTGTGGGACGAGAACAACGCGGAGTTCCAGTGCGCCGGAGTCAATATGGTCCGCCATACAATCCGGCCGAGAGGTATGCTTCAGCCAGGATACTCTAATGCTCCTAAACTCATCTTCGTTGTCCGAGGCTCCGGCTTGCGAGGCGTTGCCATTCCCGGCTGTCCAGAGACTTACCAGACCGATATGCGGAGATCGGAATCCGCGGGATCCGCGTTCAGAGACCAACACCAGAAGATCCGCGAGTTCAGAGAGGGAGATCTCCTCGTTCTCCCGGCCGGAGTTTCTCACTGGATCTATAACCGAGGACAATCTGATCTCATCTTGATCGTATTCGCCGACACTCGGAATGTCGCCAACCAAATCGATCCCTACCTCAGAGTAAGTTGAACAAAAACAGAGTATAACGATTTAATCACTGATTTGAAAAAGAAGTCGCTGAGATTCTTTGGTGAATTTGTTTCAGAAATTCCATCTCGCCGGTAGGCCAGAGCAGATAGAAAGAGGCGTGGAGGAATGGGAAAGAAGAGGCCGCAGGGGATCCGCCAGCGAGAAATCAGGCAATGTGTTCAGCGGATTTGCAGACGAACTTCTGGAGGAAGCTTTCCAGATCGACAGTGGATTGGTGAGGCGGCTGAAGGGAGAAGACGACGAGAGGGACAGGATCGTGCAAGTCGAGGAAGATTTCGAGGTGCTCATGCCGGAGAAAGATGAACAAGAGAGACAAAGAGGAAGATACATCGAATCAGAATCGGACAATGGCTTAGAAGAAACCATCTGCACACTCCGATTGAAGCACAACATCGGCCGATCTGAACGCGCCGACGTGTTCAACCCTCGCGGCGGCCGCATCTCCACCGCCAACTACCATAACCTCCCTGCCCTCCGCCAAGTCCGCCTCAGCGCCGAACGAGGAGTCCTCTACAGCGTAAGCAGAACCGAATTTAACTCTCAAATATTTCCCCTGATTCTGAAACCCCAACAATGGTAATTTCATCAAATCATCCATTTCAAAAACCCTAACAATGATTTGATTTCTTCAAATCTCCAATTTCTAAAACCCTAACACAACAACACTAATTTCTTCAAAACCCTAACCATGATGATTTCTTCAAATTTCCAATTAAAAAAAAAAAAAAACCCCTAACAACGATTCGTCAACGCTAAAATCTTCCAAATCTCGAATTTCAACACAAAGCCTAATACCTTAAAATTTCCACCTGCAGAACGCGCTCGTAGCACCGCACTACACAGTGAACAGCCACACGGTGGTGTACGCGACGAGAGGCAGCGCGAGAGTGCAAGTCGTGGACAACTACGGACAGTCGGTGTTCGACGGCGAGCTCCGGGAAGGACAGGTACTGACGATTCCACAGAACTTCGTGGTGATAAAACGGGCGAGCGACAGAGGATTCGAGTGGATCGCATTCAAGACGAACGACAACGCGGTGACTAACCTGCTGGCGGGGCGAGCTTCGCAGATGAGGCTGTTGCCGTTGGGAGTTCTGTCGAGCTCGTACCGGATCTCGAGGGAGGAGGCGCAGAGGCTCAAGTACGGGCAGCAGGAGATGAGGGTGCTGAGCCCCGGAAGGTCGCAGGGAAGAAGAGACTGAAGCGAAGAGAAGAAGAAGAAGAAATGGAAATATATGTAGTAGTAATGTAATTTAGGGAATAAAGAGCGAGCGAGCTTTCAGATGACGCCGCCGACGAGAGGTGAAATGTGAGCCAGCTTGTTACCGGCCGGAAAAAATGGAGGAATCTCTCAGAAAGACACTGAGTTTTAATAATAAAAATAATAATATTTGCCTTCTTTTCTTCAACCTCACGCTTTCTAATTTCAAATTAAAATATAATATTCCATGTTTTGCGGGCTTTCTTGGTCGTTGTTTTTTTTTTATTTGGAGAGAAAAAAAAAATGTCATATCATTTTGCCTACCTCATAAGACTCGCATTTAAGAGCGAGGTGGAGCGAGAGCAAGATTGGAGAGACATTGTGGTCGTTAGTACAATTTTATGTCGTTGATTTATGCTCTTGTTGGCAAATAACATATATCCTTAA

mRNA sequence

AGATTCCTACATACCGATATGTAGCAGTGGAAAAATTTCTAGTACTACGATGGAAAATTTCATCCATTGTTTATCATGTAAGAACTATTTAAAAATGGTGTGCGACCCATGAAATAAAATATTATGTTAGTAATGCTCATTAAATGATGTGTCAAATGCTGAATGGATAGAGTAAATACTACAGAAAGTTTTTTCCTTGTATTTTTCTCTAACATTATTCTCTCTCCACTCCTTCTTCTTCATTCTTTCATCTCTCCCCTCCTACTTTGTGCCTTCTCTTCTCCATTTGTTGTCTCTTATTTGAAAAAAAAAAAAAAACCAATGTAAAAGAAAAACATGCAAAATTTACCAAGTTATAAATCACGTACCTTTAAAGTGGACAAAGATTGAGCTGGATGACCCTGAAGAACAACGAGTCAGAACCGCCATGGCAACTGGTGCGTCTCTTCATGAATCCCTCCACCACGTATTCTCTTACCGCCACCTCCATCCATTTGTCACCGCCATTCCTCCTCCGGCCACCTATAAATACTCCTCCGCCTTCTTCTCCGTTCCTCACATTCACAACAATTACCGATCAGCCTTCTCTTCTCCATACTCATGGCTCGCCCTTCTTTTCTTTCCTTCTTATGTCTAGCAGTTTTCATCCATGCCTGCCTCGCTCAGATTGAGCAGATCCCTTGGGAGTTCCAAGGCGGCGAAATGAGGCAACAGCACCGCTTCCAATCTTCCAGAGCCTGTCGTCTCGAGAATCTCCGAGCTCACGAGCCCGTTCGCAGGGCGGAGGCGGAGGCCGGTTTCACCGAAGTGTGGGACGAGAACAACGCGGAGTTCCAGTGCGCCGGAGTCAATATGGTCCGCCATACAATCCGGCCGAGAGGTATGCTTCAGCCAGGATACTCTAATGCTCCTAAACTCATCTTCGTTGTCCGAGGCTCCGGCTTGCGAGGCGTTGCCATTCCCGGCTGTCCAGAGACTTACCAGACCGATATGCGGAGATCGGAATCCGCGGGATCCGCGTTCAGAGACCAACACCAGAAGATCCGCGAGTTCAGAGAGGGAGATCTCCTCGTTCTCCCGGCCGGAGTTTCTCACTGGATCTATAACCGAGGACAATCTGATCTCATCTTGATCGTATTCGCCGACACTCGGAATGTCGCCAACCAAATCGATCCCTACCTCAGAAAATTCCATCTCGCCGGTAGGCCAGAGCAGATAGAAAGAGGCGTGGAGGAATGGGAAAGAAGAGGCCGCAGGGGATCCGCCAGCGAGAAATCAGGCAATGTGTTCAGCGGATTTGCAGACGAACTTCTGGAGGAAGCTTTCCAGATCGACAGTGGATTGGTGAGGCGGCTGAAGGGAGAAGACGACGAGAGGGACAGGATCGTGCAAGTCGAGGAAGATTTCGAGGTGCTCATGCCGGAGAAAGATGAACAAGAGAGACAAAGAGGAAGATACATCGAATCAGAATCGGACAATGGCTTAGAAGAAACCATCTGCACACTCCGATTGAAGCACAACATCGGCCGATCTGAACGCGCCGACGTGTTCAACCCTCGCGGCGGCCGCATCTCCACCGCCAACTACCATAACCTCCCTGCCCTCCGCCAAGTCCGCCTCAGCGCCGAACGAGGAGTCCTCTACAGCAACGCGCTCGTAGCACCGCACTACACAGTGAACAGCCACACGGTGGTGTACGCGACGAGAGGCAGCGCGAGAGTGCAAGTCGTGGACAACTACGGACAGTCGGTGTTCGACGGCGAGCTCCGGGAAGGACAGGTACTGACGATTCCACAGAACTTCGTGGTGATAAAACGGGCGAGCGACAGAGGATTCGAGTGGATCGCATTCAAGACGAACGACAACGCGGTGACTAACCTGCTGGCGGGGCGAGCTTCGCAGATGAGGCTGTTGCCGTTGGGAGTTCTGTCGAGCTCGTACCGGATCTCGAGGGAGGAGGCGCAGAGGCTCAAGTACGGGCAGCAGGAGATGAGGGTGCTGAGCCCCGGAAGGTCGCAGGGAAGAAGAGACTGAAGCGAAGAGAAGAAGAAGAAGAAATGGAAATATATGTAGTAGTAATGTAATTTAGGGAATAAAGAGCGAGCGAGCTTTCAGATGACGCCGCCGACGAGAGGTGAAATGTGAGCCAGCTTGTTACCGGCCGGAAAAAATGGAGGAATCTCTCAGAAAGACACTGAGTTTTAATAATAAAAATAATAATATTTGCCTTCTTTTCTTCAACCTCACGCTTTCTAATTTCAAATTAAAATATAATATTCCATGTTTTGCGGGCTTTCTTGGTCGTTGTTTTTTTTTTATTTGGAGAGAAAAAAAAAATGTCATATCATTTTGCCTACCTCATAAGACTCGCATTTAAGAGCGAGGTGGAGCGAGAGCAAGATTGGAGAGACATTGTGGTCGTTAGTACAATTTTATGTCGTTGATTTATGCTCTTGTTGGCAAATAACATATATCCTTAA

Coding sequence (CDS)

ATGGCTCGCCCTTCTTTTCTTTCCTTCTTATGTCTAGCAGTTTTCATCCATGCCTGCCTCGCTCAGATTGAGCAGATCCCTTGGGAGTTCCAAGGCGGCGAAATGAGGCAACAGCACCGCTTCCAATCTTCCAGAGCCTGTCGTCTCGAGAATCTCCGAGCTCACGAGCCCGTTCGCAGGGCGGAGGCGGAGGCCGGTTTCACCGAAGTGTGGGACGAGAACAACGCGGAGTTCCAGTGCGCCGGAGTCAATATGGTCCGCCATACAATCCGGCCGAGAGGTATGCTTCAGCCAGGATACTCTAATGCTCCTAAACTCATCTTCGTTGTCCGAGGCTCCGGCTTGCGAGGCGTTGCCATTCCCGGCTGTCCAGAGACTTACCAGACCGATATGCGGAGATCGGAATCCGCGGGATCCGCGTTCAGAGACCAACACCAGAAGATCCGCGAGTTCAGAGAGGGAGATCTCCTCGTTCTCCCGGCCGGAGTTTCTCACTGGATCTATAACCGAGGACAATCTGATCTCATCTTGATCGTATTCGCCGACACTCGGAATGTCGCCAACCAAATCGATCCCTACCTCAGAAAATTCCATCTCGCCGGTAGGCCAGAGCAGATAGAAAGAGGCGTGGAGGAATGGGAAAGAAGAGGCCGCAGGGGATCCGCCAGCGAGAAATCAGGCAATGTGTTCAGCGGATTTGCAGACGAACTTCTGGAGGAAGCTTTCCAGATCGACAGTGGATTGGTGAGGCGGCTGAAGGGAGAAGACGACGAGAGGGACAGGATCGTGCAAGTCGAGGAAGATTTCGAGGTGCTCATGCCGGAGAAAGATGAACAAGAGAGACAAAGAGGAAGATACATCGAATCAGAATCGGACAATGGCTTAGAAGAAACCATCTGCACACTCCGATTGAAGCACAACATCGGCCGATCTGAACGCGCCGACGTGTTCAACCCTCGCGGCGGCCGCATCTCCACCGCCAACTACCATAACCTCCCTGCCCTCCGCCAAGTCCGCCTCAGCGCCGAACGAGGAGTCCTCTACAGCAACGCGCTCGTAGCACCGCACTACACAGTGAACAGCCACACGGTGGTGTACGCGACGAGAGGCAGCGCGAGAGTGCAAGTCGTGGACAACTACGGACAGTCGGTGTTCGACGGCGAGCTCCGGGAAGGACAGGTACTGACGATTCCACAGAACTTCGTGGTGATAAAACGGGCGAGCGACAGAGGATTCGAGTGGATCGCATTCAAGACGAACGACAACGCGGTGACTAACCTGCTGGCGGGGCGAGCTTCGCAGATGAGGCTGTTGCCGTTGGGAGTTCTGTCGAGCTCGTACCGGATCTCGAGGGAGGAGGCGCAGAGGCTCAAGTACGGGCAGCAGGAGATGAGGGTGCTGAGCCCCGGAAGGTCGCAGGGAAGAAGAGACTGA

Protein sequence

MARPSFLSFLCLAVFIHACLAQIEQIPWEFQGGEMRQQHRFQSSRACRLENLRAHEPVRRAEAEAGFTEVWDENNAEFQCAGVNMVRHTIRPRGMLQPGYSNAPKLIFVVRGSGLRGVAIPGCPETYQTDMRRSESAGSAFRDQHQKIREFREGDLLVLPAGVSHWIYNRGQSDLILIVFADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSASEKSGNVFSGFADELLEEAFQIDSGLVRRLKGEDDERDRIVQVEEDFEVLMPEKDEQERQRGRYIESESDNGLEETICTLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSAERGVLYSNALVAPHYTVNSHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFVVIKRASDRGFEWIAFKTNDNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQEMRVLSPGRSQGRRD
Homology
BLAST of Lcy01g003380 vs. ExPASy Swiss-Prot
Match: P13744 (11S globulin subunit beta OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 823.9 bits (2127), Expect = 9.1e-238
Identity = 407/480 (84.79%), Postives = 448/480 (93.33%), Query Frame = 0

Query: 1   MARPSFLSFLCLAVFIHACLAQIE-QIPWEFQGGEMRQQHRFQSSRACRLENLRAHEPVR 60
           MAR S  +FLCLAVFI+ CL+QIE Q PWEFQG E+ QQHR+QS RACRLENLRA +PVR
Sbjct: 1   MARSSLFTFLCLAVFINGCLSQIEQQSPWEFQGSEVWQQHRYQSPRACRLENLRAQDPVR 60

Query: 61  RAEAEAGFTEVWDENNAEFQCAGVNMVRHTIRPRGMLQPGYSNAPKLIFVVRGSGLRGVA 120
           RAEAEA FTEVWD++N EFQCAGVNM+RHTIRP+G+L PG+SNAPKLIFV +G G+RG+A
Sbjct: 61  RAEAEAIFTEVWDQDNDEFQCAGVNMIRHTIRPKGLLLPGFSNAPKLIFVAQGFGIRGIA 120

Query: 121 IPGCPETYQTDMRRSESAGSAFRDQHQKIREFREGDLLVLPAGVSHWIYNRGQSDLILIV 180
           IPGC ETYQTD+RRS+SAGSAF+DQHQKIR FREGDLLV+PAGVSHW+YNRGQSDL+LIV
Sbjct: 121 IPGCAETYQTDLRRSQSAGSAFKDQHQKIRPFREGDLLVVPAGVSHWMYNRGQSDLVLIV 180

Query: 181 FADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSASEKSGNVFSGFADELLE 240
           FADTRNVANQIDPYLRKF+LAGRPEQ+ERGVEEWER  R+GS+ EKSGN+FSGFADE LE
Sbjct: 181 FADTRNVANQIDPYLRKFYLAGRPEQVERGVEEWERSSRKGSSGEKSGNIFSGFADEFLE 240

Query: 241 EAFQIDSGLVRRLKGEDDERDRIVQVEEDFEVLMPEKDEQERQRGRYI--ESESDNGLEE 300
           EAFQID GLVR+LKGEDDERDRIVQV+EDFEVL+PEKDE+ER RGRYI  ESES+NGLEE
Sbjct: 241 EAFQIDGGLVRKLKGEDDERDRIVQVDEDFEVLLPEKDEEERSRGRYIESESESENGLEE 300

Query: 301 TICTLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSAERGVLYSNALVAPHY 360
           TICTLRLK NIGRS RADVFNPRGGRISTANYH LP LRQVRLSAERGVLYSNA+VAPHY
Sbjct: 301 TICTLRLKQNIGRSVRADVFNPRGGRISTANYHTLPILRQVRLSAERGVLYSNAMVAPHY 360

Query: 361 TVNSHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFVVIKRASDRGFEWIAF 420
           TVNSH+V+YATRG+ARVQVVDN+GQSVFDGE+REGQVL IPQNFVVIKRASDRGFEWIAF
Sbjct: 361 TVNSHSVMYATRGNARVQVVDNFGQSVFDGEVREGQVLMIPQNFVVIKRASDRGFEWIAF 420

Query: 421 KTNDNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQEMRVLSPGRSQGRRD 478
           KTNDNA+TNLLAGR SQMR+LPLGVLS+ YRISREEAQRLKYGQQEMRVLSPGRSQGRR+
Sbjct: 421 KTNDNAITNLLAGRVSQMRMLPLGVLSNMYRISREEAQRLKYGQQEMRVLSPGRSQGRRE 480

BLAST of Lcy01g003380 vs. ExPASy Swiss-Prot
Match: A0A1L6K371 (11S globulin OS=Juglans nigra OX=16719 PE=1 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 5.5e-126
Identity = 257/513 (50.10%), Postives = 330/513 (64.33%), Query Frame = 0

Query: 1   MARPSFLSF-LCLAVFIHACLAQIEQIPWEFQGGEMRQQHRFQSSRACRLENLRAHEPVR 60
           MA+P  LS  LCL   ++ CLAQ         GG  RQQ RF     C+L+ L A EP  
Sbjct: 1   MAKPILLSISLCLVALVNGCLAQ--------SGG--RQQPRFGE---CKLKRLVALEPSN 60

Query: 61  RAEAEAGFTEVWDENNAEFQCAGVNMVRHTIRPRGMLQPGYSNAPKLIFVVRGSGLRGVA 120
           R EAEAG  E WD NN +FQCAGV +VR TI P G+L P YSNAP+L+++V+G G+ GV 
Sbjct: 61  RIEAEAGVIESWDPNNQQFQCAGVAVVRRTIEPNGLLLPQYSNAPQLLYIVKGRGITGVL 120

Query: 121 IPGCPETY------QTDMRRSESAGSAFRDQHQKIREFREGDLLVLPAGVSHWIYNRGQS 180
            PGCPET+      Q+ +R S  + S  RD+HQKIR FREGD++  PAGV+HW YN G +
Sbjct: 121 FPGCPETFEESQQGQSRIRPSLRSASFQRDRHQKIRHFREGDVIAFPAGVAHWCYNDGDT 180

Query: 181 DLILIVFADTRNVANQIDPYLRKFHLAGRP------------EQIERGVEEWERRGRRGS 240
            ++ +   DT N ANQ+D   R F+LAG P            EQ  R  +  +R G  G 
Sbjct: 181 PVVAVALMDTTNNANQLDQNPRNFYLAGNPDDEFRPQGQQEYEQHRRQQQHQQRHGEPGQ 240

Query: 241 ASEKSG-NVFSGFADELLEEAFQIDSGLVRRLKGEDDERDRIVQVE-EDFEVLMP--EKD 300
               SG NVFSGF  + L +AF +D+   RRL+ E+D R  IV+VE    +V+ P   ++
Sbjct: 241 QQRGSGNNVFSGFDADFLADAFNVDTETARRLQSENDHRRSIVRVEGRQLQVIRPRWSRE 300

Query: 301 EQER----QRGRYIESES-----------DNGLEETICTLRLKHNIGRSERADVFNPRGG 360
           EQER    +R R  ESES           DNGLEETICTLRL+ NIG   RAD++    G
Sbjct: 301 EQEREERKERERERESESERRQSRRGGRDDNGLEETICTLRLRENIGDPSRADIYTEEAG 360

Query: 361 RISTANYHNLPALRQVRLSAERGVLYSNALVAPHYTVNSHTVVYATRGSARVQVVDNYGQ 420
           RISTAN H LP LR ++LSAERG LYS+AL  PH+ +N+H+VVYA RG A VQVVDN+GQ
Sbjct: 361 RISTANSHTLPVLRWLQLSAERGALYSDALYVPHWNLNAHSVVYALRGRAEVQVVDNFGQ 420

Query: 421 SVFDGELREGQVLTIPQNFVVIKRASDRGFEWIAFKTNDNAVTNLLAGRASQMRLLPLGV 476
           +VFD ELREGQ+LTIPQNF V+KRA + GFEW++FKTN+NA+ + LAGR S +R LP  V
Sbjct: 421 TVFDDELREGQLLTIPQNFAVVKRARNEGFEWVSFKTNENAMVSPLAGRTSAIRALPEEV 480

BLAST of Lcy01g003380 vs. ExPASy Swiss-Prot
Match: B5KVH4 (11S globulin seed storage protein 1 OS=Carya illinoinensis OX=32201 PE=1 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 4.7e-125
Identity = 253/511 (49.51%), Postives = 326/511 (63.80%), Query Frame = 0

Query: 1   MARPSFLS-FLCLAV--FIHACLAQIEQIPWEFQGGEMRQQHRFQSSRACRLENLRAHEP 60
           MA+P  LS +LCL +    + CLAQ         GG  RQQH+F     C+L  L A EP
Sbjct: 1   MAKPILLSIYLCLIIVALFNGCLAQ--------SGG--RQQHKFGQ---CQLNRLDALEP 60

Query: 61  VRRAEAEAGFTEVWDENNAEFQCAGVNMVRHTIRPRGMLQPGYSNAPKLIFVVRGSGLRG 120
             R EAEAG  E WD N+ + QCAGV +VR TI P G+L P YSNAP+L+++ RG G+ G
Sbjct: 61  TNRIEAEAGVIESWDPNHQQLQCAGVAVVRRTIEPNGLLLPHYSNAPQLVYIARGRGITG 120

Query: 121 VAIPGCPETYQTDMRRSESA--GSAFRDQHQKIREFREGDLLVLPAGVSHWIYNRGQSDL 180
           V  PGCPET++   R+S+        +D+HQKIR FREGD++  PAGV+HW YN G S +
Sbjct: 121 VLFPGCPETFEESQRQSQQGQRREFQQDRHQKIRHFREGDIIAFPAGVAHWCYNDGSSPV 180

Query: 181 ILIVFADTRNVANQIDPYLRKFHLAGRP------------EQIERGVEEWERRGRRGSAS 240
           + I   DT N ANQ+D   R F+LAG P            EQ  R  +  +RRG  G   
Sbjct: 181 VAIFLLDTHNNANQLDQNPRNFYLAGNPDDEFRPQGQQEYEQHRRQQQHQQRRGEHGEQQ 240

Query: 241 EKSG-NVFSGFADELLEEAFQIDSGLVRRLKGEDDERDRIVQVE-EDFEVLMP------- 300
              G NVFSGF  E L +AF +D+   RRL+ E+D R  IV+VE    +V+ P       
Sbjct: 241 RDLGNNVFSGFDAEFLADAFNVDTETARRLQSENDHRGSIVRVEGRQLQVIRPRWSREEQ 300

Query: 301 -EKDEQERQRGRYIESE---------SDNGLEETICTLRLKHNIGRSERADVFNPRGGRI 360
             ++ +ER+R R  ESE          DNGLEETICTL L+ NIG   RAD++    GRI
Sbjct: 301 EHEERKERERERESESERRQSRRGGRDDNGLEETICTLSLRENIGDPSRADIYTEEAGRI 360

Query: 361 STANYHNLPALRQVRLSAERGVLYSNALVAPHYTVNSHTVVYATRGSARVQVVDNYGQSV 420
           ST N HNLP LR ++LSAERG LYS+AL  PH+ +N+H+VVYA RG A VQVVDN+GQ+V
Sbjct: 361 STVNSHNLPILRWLQLSAERGALYSDALYVPHWNLNAHSVVYALRGRAEVQVVDNFGQTV 420

Query: 421 FDGELREGQVLTIPQNFVVIKRASDRGFEWIAFKTNDNAVTNLLAGRASQMRLLPLGVLS 476
           FD ELREGQ+LTIPQNF V+KRA D GFEW++FKTN+NA+ + LAGR S +R LP  VL 
Sbjct: 421 FDDELREGQLLTIPQNFAVVKRARDEGFEWVSFKTNENAMVSPLAGRTSAIRALPEEVLV 480

BLAST of Lcy01g003380 vs. ExPASy Swiss-Prot
Match: Q2TPW5 (11S globulin seed storage protein Jug r 4 OS=Juglans regia OX=51240 PE=1 SV=1)

HSP 1 Score: 448.7 bits (1153), Expect = 8.0e-125
Identity = 255/514 (49.61%), Postives = 335/514 (65.18%), Query Frame = 0

Query: 1   MARPSFLS---FLCLAVFIHACLAQIEQIPWEFQGGEMRQQHRFQSSRACRLENLRAHEP 60
           MA+P  LS   FL +A+F + CLAQ         GG  RQQ +F     C+L  L A EP
Sbjct: 1   MAKPILLSIYLFLIVALF-NGCLAQ--------SGG--RQQQQFGQ---CQLNRLDALEP 60

Query: 61  VRRAEAEAGFTEVWDENNAEFQCAGVNMVRHTIRPRGMLQPGYSNAPKLIFVVRGSGLRG 120
             R EAEAG  E WD NN +FQCAGV +VR TI P G+L P YSNAP+L+++ RG G+ G
Sbjct: 61  TNRIEAEAGVIESWDPNNQQFQCAGVAVVRRTIEPNGLLLPQYSNAPQLVYIARGRGITG 120

Query: 121 VAIPGCPETYQTDMRRSESAGSA--FRDQHQKIREFREGDLLVLPAGVSHWIYNRGQSDL 180
           V  PGCPET++   R+S+   S    +D+HQKIR FREGD++  PAGV+HW YN G + +
Sbjct: 121 VLFPGCPETFEESQRQSQQGQSREFQQDRHQKIRHFREGDIIAFPAGVAHWSYNDGSNPV 180

Query: 181 ILIVFADTRNVANQIDPYLRKFHLAGRPEQIER--GVEEWERRGRRGSASEKSG------ 240
           + I   DT N ANQ+D   R F+LAG P+   R  G +E+E+  R+    ++ G      
Sbjct: 181 VAISLLDTNNNANQLDQNPRNFYLAGNPDDEFRPQGQQEYEQHRRQQQRQQRPGEHGQQQ 240

Query: 241 -----NVFSGFADELLEEAFQIDSGLVRRLKGEDDERDRIVQVE-EDFEVLMP--EKDEQ 300
                NVFSGF  + L +AF +D+   RRL+ E+D R  IV+VE    +V+ P   ++EQ
Sbjct: 241 RGLGNNVFSGFDADFLADAFNVDTETARRLQSENDHRRSIVRVEGRQLQVIRPRWSREEQ 300

Query: 301 ER----QRGRYIESES-----------DNGLEETICTLRLKHNIGRSERADVFNPRGGRI 360
           ER    +R R  ESES           DNGLEETICTLRL+ NIG   RAD++    GRI
Sbjct: 301 EREERKERERERESESERRQSRRGGRDDNGLEETICTLRLRENIGDPSRADIYTEEAGRI 360

Query: 361 STANYHNLPALRQVRLSAERGVLYSNALVAPHYTVNSHTVVYATRGSARVQVVDNYGQSV 420
           ST N H LP LR ++LSAERG LYS+AL  PH+ +N+H+VVYA RG A VQVVDN+GQ+V
Sbjct: 361 STVNSHTLPVLRWLQLSAERGALYSDALYVPHWNLNAHSVVYALRGRAEVQVVDNFGQTV 420

Query: 421 FDGELREGQVLTIPQNFVVIKRASDRGFEWIAFKTNDNAVTNLLAGRASQMRLLPLGVLS 477
           FD ELREGQ+LTIPQNF V+KRA + GFEW++FKTN+NA+ + LAGR S +R LP  VL+
Sbjct: 421 FDDELREGQLLTIPQNFAVVKRARNEGFEWVSFKTNENAMVSPLAGRTSAIRALPEEVLA 480

BLAST of Lcy01g003380 vs. ExPASy Swiss-Prot
Match: Q8GZP6 (11S globulin seed storage protein Ana o 2.0101 (Fragment) OS=Anacardium occidentale OX=171929 PE=1 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 2.0e-120
Identity = 221/478 (46.23%), Postives = 310/478 (64.85%), Query Frame = 0

Query: 10  LCLAVFIHACLAQIEQIPWEFQGGEMRQQHRFQSSRACRLENLRAHEPVRRAEAEAGFTE 69
           +C  +  H CLA                +  +Q    C+++ L A EP  R E EAG  E
Sbjct: 3   VCFLILFHGCLA---------------SRQEWQQQDECQIDRLDALEPDNRVEYEAGTVE 62

Query: 70  VWDENNAEFQCAGVNMVRHTIRPRGMLQPGYSNAPKLIFVVRGSGLRGVAIPGCPETYQT 129
            WD N+ +F+CAGV +VRHTI+P G+L P YSNAP+LI+VV+G G+ G++ PGCPETYQ 
Sbjct: 63  AWDPNHEQFRCAGVALVRHTIQPNGLLLPQYSNAPQLIYVVQGEGMTGISYPGCPETYQA 122

Query: 130 DMR-RSESAGSAFRDQHQKIREFREGDLLVLPAGVSHWIYNRGQSDLILIVFADTRNVAN 189
             + R +     F+D+HQKIR FR GD++ +PAGV+HW YN G S ++ +   D  N  N
Sbjct: 123 PQQGRQQGQSGRFQDRHQKIRRFRRGDIIAIPAGVAHWCYNEGNSPVVTVTLLDVSNSQN 182

Query: 190 QIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSASEKSGNVFSGFADELLEEAFQIDSGL 249
           Q+D   RKFHLAG P+ + +  ++ + RGR         N+FSGF  ELL EAFQ+D  L
Sbjct: 183 QLDRTPRKFHLAGNPKDVFQQQQQHQSRGR---------NLFSGFDTELLAEAFQVDERL 242

Query: 250 VRRLKGEDDERDRIVQVEED-FEVLMPEKDEQERQRGRYIESES--------DNGLEETI 309
           +++LK ED+ R  IV+V++D   V+ P + + ER      ESE         DNG+EETI
Sbjct: 243 IKQLKSEDN-RGGIVKVKDDELRVIRPSRSQSERGSESEEESEDEKRRWGQRDNGIEETI 302

Query: 310 CTLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSAERGVLYSNALVAPHYTV 369
           CT+RLK NI    RAD++ P  GR++T N  NLP L+ ++LS E+GVLY NALV PH+ +
Sbjct: 303 CTMRLKENINDPARADIYTPEVGRLTTLNSLNLPILKWLQLSVEKGVLYKNALVLPHWNL 362

Query: 370 NSHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFVVIKRASDRGFEWIAFKT 429
           NSH+++Y  +G  +VQVVDN+G  VFDGE+REGQ+L +PQNF V+KRA +  FEWI+FKT
Sbjct: 363 NSHSIIYGCKGKGQVQVVDNFGNRVFDGEVREGQMLVVPQNFAVVKRAREERFEWISFKT 422

Query: 430 NDNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQEMRVLSPGRSQGRRD 478
           ND A+T+ LAGR S +  +P  VL+++++ISRE+A+++K+  Q+  + S   S   RD
Sbjct: 423 NDRAMTSPLAGRTSVLGGMPEEVLANAFQISREDARKIKFNNQQTTLTSGESSHHMRD 455

BLAST of Lcy01g003380 vs. ExPASy TrEMBL
Match: A0A6J1HL28 (11S globulin subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111464525 PE=3 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 9.5e-238
Identity = 410/480 (85.42%), Postives = 449/480 (93.54%), Query Frame = 0

Query: 1   MARPSFLSFLCLAVFIHACLAQIE-QIPWEFQGGEMRQQHRFQSSRACRLENLRAHEPVR 60
           MAR S  +FLCLAV I+ CL+QIE Q PWEFQG E+ QQHR+QS RACRLENLRA EPVR
Sbjct: 1   MARSSLFTFLCLAVLINGCLSQIEQQSPWEFQGSEVWQQHRYQSPRACRLENLRAQEPVR 60

Query: 61  RAEAEAGFTEVWDENNAEFQCAGVNMVRHTIRPRGMLQPGYSNAPKLIFVVRGSGLRGVA 120
           RAEAEAGFTEVWD++N EFQCAGVNM+RHTIRP+G+L PG+SNAPKL+FV +G G+RG+A
Sbjct: 61  RAEAEAGFTEVWDQDNDEFQCAGVNMIRHTIRPKGLLLPGFSNAPKLVFVAQGFGIRGIA 120

Query: 121 IPGCPETYQTDMRRSESAGSAFRDQHQKIREFREGDLLVLPAGVSHWIYNRGQSDLILIV 180
           IPGC ETYQTD+RRS+SAGSAFRDQHQKIR FREGDLLV+PAGVSHW+YNRGQSDL+LIV
Sbjct: 121 IPGCAETYQTDLRRSQSAGSAFRDQHQKIRPFREGDLLVVPAGVSHWMYNRGQSDLVLIV 180

Query: 181 FADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSASEKSGNVFSGFADELLE 240
           FADTRNVANQIDPYLRKF+LAGRPEQ+ERGVEEWER  R+GS+ EKSGN+FSGFADE LE
Sbjct: 181 FADTRNVANQIDPYLRKFYLAGRPEQVERGVEEWERSSRKGSSGEKSGNLFSGFADEFLE 240

Query: 241 EAFQIDSGLVRRLKGEDDERDRIVQVEEDFEVLMPEKDEQERQRGRYI--ESESDNGLEE 300
           EAFQID GLVR+LKGEDDERDRIVQV+EDFEVL+PEKDE+ER RGRYI  ESES+NGLEE
Sbjct: 241 EAFQIDGGLVRKLKGEDDERDRIVQVDEDFEVLLPEKDEEERSRGRYIESESESENGLEE 300

Query: 301 TICTLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSAERGVLYSNALVAPHY 360
           TICTLRLKHNIGRSERADVFNPRGGRISTANYH LP LRQVRLSAERGVLYSNA+VAPHY
Sbjct: 301 TICTLRLKHNIGRSERADVFNPRGGRISTANYHTLPVLRQVRLSAERGVLYSNAMVAPHY 360

Query: 361 TVNSHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFVVIKRASDRGFEWIAF 420
           TVNSH+V+YATRGSARVQVVDN+GQSVFDGE+REGQVL IPQNFV IKRASDRGFEWIAF
Sbjct: 361 TVNSHSVMYATRGSARVQVVDNFGQSVFDGEVREGQVLMIPQNFVAIKRASDRGFEWIAF 420

Query: 421 KTNDNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQEMRVLSPGRSQGRRD 478
           KTNDNA+TNLLAGR SQMR+LPLGVLS+ YRISREEAQRLKYGQQEMRVLSPGRSQGRR+
Sbjct: 421 KTNDNAITNLLAGRVSQMRMLPLGVLSNMYRISREEAQRLKYGQQEMRVLSPGRSQGRRE 480

BLAST of Lcy01g003380 vs. ExPASy TrEMBL
Match: A0A6J1KMS5 (11S globulin subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111494748 PE=3 SV=1)

HSP 1 Score: 830.5 bits (2144), Expect = 3.6e-237
Identity = 409/480 (85.21%), Postives = 450/480 (93.75%), Query Frame = 0

Query: 1   MARPSFLSFLCLAVFIHACLAQIE-QIPWEFQGGEMRQQHRFQSSRACRLENLRAHEPVR 60
           MAR S  +FLCLAVFI+ CL+QIE Q PWEFQG E+ QQHR+QS RACRLENLRA +PVR
Sbjct: 1   MARSSLFTFLCLAVFINGCLSQIEQQSPWEFQGSEVWQQHRYQSPRACRLENLRAQDPVR 60

Query: 61  RAEAEAGFTEVWDENNAEFQCAGVNMVRHTIRPRGMLQPGYSNAPKLIFVVRGSGLRGVA 120
           RAEAEAGFTEVWD++N EFQCAGVNM+RHTIRP+G+L PG+SNAPKLIFV +G G+RG+A
Sbjct: 61  RAEAEAGFTEVWDQDNDEFQCAGVNMIRHTIRPKGLLLPGFSNAPKLIFVAQGFGIRGIA 120

Query: 121 IPGCPETYQTDMRRSESAGSAFRDQHQKIREFREGDLLVLPAGVSHWIYNRGQSDLILIV 180
           IPGC ETYQTD+RRS+SAGSAF+DQHQKIR FREGDLLV+PAGVSHW+YNRGQSDL+LIV
Sbjct: 121 IPGCAETYQTDLRRSQSAGSAFKDQHQKIRPFREGDLLVVPAGVSHWMYNRGQSDLVLIV 180

Query: 181 FADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSASEKSGNVFSGFADELLE 240
           FADTRNVANQIDPYLRKF+LAGRPEQ+ERGVEEWER  R+GS+ EKSGN+FSGFADE LE
Sbjct: 181 FADTRNVANQIDPYLRKFYLAGRPEQVERGVEEWERSSRKGSSGEKSGNIFSGFADEFLE 240

Query: 241 EAFQIDSGLVRRLKGEDDERDRIVQVEEDFEVLMPEKDEQERQRGRYI--ESESDNGLEE 300
           EAFQID GLVR+LKGEDDERDRIVQV+EDFEVL+PEKDE+ER RGRYI  ESES+NGLEE
Sbjct: 241 EAFQIDGGLVRKLKGEDDERDRIVQVDEDFEVLLPEKDEEERSRGRYIESESESENGLEE 300

Query: 301 TICTLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSAERGVLYSNALVAPHY 360
           TICTLRLK NIGRSERADVFNPRGGRISTANYH LP LRQVRLSAERGVLYSNA+VAPHY
Sbjct: 301 TICTLRLKQNIGRSERADVFNPRGGRISTANYHTLPILRQVRLSAERGVLYSNAMVAPHY 360

Query: 361 TVNSHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFVVIKRASDRGFEWIAF 420
           TVNSH+V+YATRG+ARVQVVDN+GQSVFDGE+REGQVL IPQNFVVIKRASDRGFEWIAF
Sbjct: 361 TVNSHSVMYATRGNARVQVVDNFGQSVFDGEVREGQVLMIPQNFVVIKRASDRGFEWIAF 420

Query: 421 KTNDNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQEMRVLSPGRSQGRRD 478
           KTNDNA+TNLLAGR SQMR+LPLGVLS+ YRISREEAQRLKYGQQEMRVLSPGRSQGRR+
Sbjct: 421 KTNDNAITNLLAGRVSQMRMLPLGVLSNMYRISREEAQRLKYGQQEMRVLSPGRSQGRRE 480

BLAST of Lcy01g003380 vs. ExPASy TrEMBL
Match: A0A6J1KVP5 (11S globulin subunit beta-like OS=Cucurbita maxima OX=3661 GN=LOC111499130 PE=3 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 1.2e-232
Identity = 405/477 (84.91%), Postives = 437/477 (91.61%), Query Frame = 0

Query: 1   MARPSFLSFLCLAVFIHACLAQIEQIPWEFQGGEMRQQHRFQSSRACRLENLRAHEPVRR 60
           MAR S L   CL VFI+ C AQ E  P EFQGGE  QQHRF S RACRLENLRA EPVRR
Sbjct: 63  MARSSLLGLSCLLVFINVCFAQFEHFPQEFQGGEAMQQHRFHSPRACRLENLRAQEPVRR 122

Query: 61  AEAEAGFTEVWDENNAEFQCAGVNMVRHTIRPRGMLQPGYSNAPKLIFVVRGSGLRGVAI 120
           AEAEAG TEVWD+ + EFQCAGVNM+RH IR RG+L PG+SNAPKLIFVV+G+G+RGVA+
Sbjct: 123 AEAEAGSTEVWDQYSEEFQCAGVNMLRHKIRSRGLLLPGFSNAPKLIFVVQGTGIRGVAL 182

Query: 121 PGCPETYQTDMRRSESAGSAFRDQHQKIREFREGDLLVLPAGVSHWIYNRGQSDLILIVF 180
           PGC ETYQTD+RRS+SAGSAFRDQHQKIR+FREGDLLV+PAGVSHWIYNRGQSDLILIVF
Sbjct: 183 PGCAETYQTDLRRSQSAGSAFRDQHQKIRQFREGDLLVVPAGVSHWIYNRGQSDLILIVF 242

Query: 181 ADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSASEKSGNVFSGFADELLEE 240
            DTRNVANQIDPYLRKF LAGRPE +ERG EE E R RRGS  EKSGN+FSGFADELLEE
Sbjct: 243 TDTRNVANQIDPYLRKFFLAGRPEMVERGAEEMEGRSRRGSVGEKSGNMFSGFADELLEE 302

Query: 241 AFQIDSGLVRRLKGEDDERDRIVQVEEDFEVLMPEKDEQERQRGRYIESESDNGLEETIC 300
           AF++DSGLVRRLKGEDDERDRIVQV+EDF+VL+PE+DEQER RGRYIESES+NG EETIC
Sbjct: 303 AFKVDSGLVRRLKGEDDERDRIVQVDEDFDVLLPERDEQERSRGRYIESESENGFEETIC 362

Query: 301 TLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSAERGVLYSNALVAPHYTVN 360
           TLRLKHNIGRSERADVFNPRGGRISTANYHNLP LRQVRLSAERGVLYSNALVAPHYTVN
Sbjct: 363 TLRLKHNIGRSERADVFNPRGGRISTANYHNLPFLRQVRLSAERGVLYSNALVAPHYTVN 422

Query: 361 SHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFVVIKRASDRGFEWIAFKTN 420
           SHTVVYATRGSARVQVVD+ GQSVF+GELREGQVL IPQNFVV+K+ASD+GFEWIAFKTN
Sbjct: 423 SHTVVYATRGSARVQVVDDSGQSVFNGELREGQVLMIPQNFVVMKQASDKGFEWIAFKTN 482

Query: 421 DNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQEMRVLSPGRSQGRRD 478
           DNA+TNLLAGRASQMR+ PLGVLS+ YRISREEAQRLKYGQQEMR+LSPGRSQGRRD
Sbjct: 483 DNAITNLLAGRASQMRMFPLGVLSNMYRISREEAQRLKYGQQEMRILSPGRSQGRRD 539

BLAST of Lcy01g003380 vs. ExPASy TrEMBL
Match: A0A6J1H609 (11S globulin subunit beta-like OS=Cucurbita moschata OX=3662 GN=LOC111460434 PE=3 SV=1)

HSP 1 Score: 807.0 bits (2083), Expect = 4.3e-230
Identity = 402/477 (84.28%), Postives = 435/477 (91.19%), Query Frame = 0

Query: 1   MARPSFLSFLCLAVFIHACLAQIEQIPWEFQGGEMRQQHRFQSSRACRLENLRAHEPVRR 60
           MAR S L   CL VFI+ C AQ    P EFQGGE  QQHRF S RACRLENLRA EPVRR
Sbjct: 1   MARSSLLGLSCLLVFINVCFAQFGHFPQEFQGGEAMQQHRFHSPRACRLENLRAQEPVRR 60

Query: 61  AEAEAGFTEVWDENNAEFQCAGVNMVRHTIRPRGMLQPGYSNAPKLIFVVRGSGLRGVAI 120
           AEAEAG TEVWD+ + EFQCAGVNM+RH IR RG+L PG+SNAPKLIFVV+G+G+RGVAI
Sbjct: 61  AEAEAGSTEVWDQYSEEFQCAGVNMLRHKIRSRGLLLPGFSNAPKLIFVVQGTGIRGVAI 120

Query: 121 PGCPETYQTDMRRSESAGSAFRDQHQKIREFREGDLLVLPAGVSHWIYNRGQSDLILIVF 180
           PGC ETYQTD+RRS+SAGSAFRDQHQKIR+FREGDLLV+PAGVSHWIYNRGQSDLILIVF
Sbjct: 121 PGCAETYQTDLRRSQSAGSAFRDQHQKIRQFREGDLLVVPAGVSHWIYNRGQSDLILIVF 180

Query: 181 ADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSASEKSGNVFSGFADELLEE 240
            DTRNVANQIDPYLRKF LAGRPE +ERG EE E R RRGS  EKSGN+FSGFADELLEE
Sbjct: 181 TDTRNVANQIDPYLRKFFLAGRPEMVERGAEEMEGRSRRGSVGEKSGNMFSGFADELLEE 240

Query: 241 AFQIDSGLVRRLKGEDDERDRIVQVEEDFEVLMPEKDEQERQRGRYIESESDNGLEETIC 300
           AF+I SGLVRRLKGEDDERDRIVQV+EDF+VL+PEKDEQER RGRYIESES+NG EETIC
Sbjct: 241 AFKIGSGLVRRLKGEDDERDRIVQVDEDFDVLLPEKDEQERSRGRYIESESENGFEETIC 300

Query: 301 TLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSAERGVLYSNALVAPHYTVN 360
           TLRLKHNIGRSERADVFNPRGGRISTANYHNLP LRQVRLSAERGVLYSNALVAPHYTVN
Sbjct: 301 TLRLKHNIGRSERADVFNPRGGRISTANYHNLPFLRQVRLSAERGVLYSNALVAPHYTVN 360

Query: 361 SHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFVVIKRASDRGFEWIAFKTN 420
           SHTV+YATRGSARVQVVD+ GQSVF+GE+R+GQVL IPQNFVV+K+ASD+GFEWIAFKTN
Sbjct: 361 SHTVLYATRGSARVQVVDDLGQSVFNGEVRKGQVLMIPQNFVVMKQASDKGFEWIAFKTN 420

Query: 421 DNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQEMRVLSPGRSQGRRD 478
           DNA+TNLLAGRAS+MR+LPLGVLS+ YRISREEAQRLKYGQQEMR+LSPG SQGRRD
Sbjct: 421 DNAITNLLAGRASEMRMLPLGVLSNMYRISREEAQRLKYGQQEMRILSPGMSQGRRD 477

BLAST of Lcy01g003380 vs. ExPASy TrEMBL
Match: A0A1S3CIE1 (11S globulin subunit beta-like OS=Cucumis melo OX=3656 GN=LOC103500763 PE=3 SV=1)

HSP 1 Score: 775.8 bits (2002), Expect = 1.1e-220
Identity = 384/479 (80.17%), Postives = 428/479 (89.35%), Query Frame = 0

Query: 1   MARPSFLSFLCLAVFIHACLAQIEQIPWE--FQGGEMRQQHRFQSSRACRLENLRAHEPV 60
           MAR S L+FLCLAVFI+ CL+Q +Q PW   FQG E RQQHRF S +AC LENLRA EP 
Sbjct: 1   MARSSLLAFLCLAVFINGCLSQTDQFPWSWGFQGSEARQQHRFHSPKACHLENLRAEEPA 60

Query: 61  RRAEAEAGFTEVWDENNAEFQCAGVNMVRHTIRPRGMLQPGYSNAPKLIFVVRGSGLRGV 120
           RR EAEAG+TE+W+ NN EFQCAGVNMVRHTIRP+G+L PG++NAPKLIFVV+G+G+RGV
Sbjct: 61  RRIEAEAGYTELWEPNNEEFQCAGVNMVRHTIRPKGLLLPGFTNAPKLIFVVQGAGIRGV 120

Query: 121 AIPGCPETYQTDMRRSESAGSAFRDQHQKIREFREGDLLVLPAGVSHWIYNRGQSDLILI 180
           A+PGCPETY+TD+RRS+S+G+ FRDQHQKIR FREGDLLVLPAGVS WIYNRGQSDLIL+
Sbjct: 121 AMPGCPETYETDLRRSQSSGN-FRDQHQKIRHFREGDLLVLPAGVSQWIYNRGQSDLILV 180

Query: 181 VFADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSASEKSGNVFSGFADELL 240
           VF DTRNVANQIDP+ RKF+LAGRPE + RG  E E R  R S  EKSGNVFSGFADE L
Sbjct: 181 VFVDTRNVANQIDPFARKFYLAGRPEMMRRG--EREGRSFRESTGEKSGNVFSGFADEFL 240

Query: 241 EEAFQIDSGLVRRLKGEDDERDRIVQVEEDFEVLMPEKDEQERQRGRYIESESDNGLEET 300
           EEAFQ+DSGLVRRLKGE+D+RDRIV  EEDF+VLMPE+D+QER RGRY+E ES+NG EET
Sbjct: 241 EEAFQVDSGLVRRLKGENDDRDRIVFTEEDFDVLMPERDDQERSRGRYVEKESENGFEET 300

Query: 301 ICTLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSAERGVLYSNALVAPHYT 360
           +CTLRLKHNIGRSE ADVFNPRGGRISTAN++NLP LRQVRLSAERGVLYSNA+ APHYT
Sbjct: 301 VCTLRLKHNIGRSEHADVFNPRGGRISTANFNNLPFLRQVRLSAERGVLYSNAISAPHYT 360

Query: 361 VNSHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFVVIKRASDRGFEWIAFK 420
           VN+HTV YATRGSARVQVVDNYGQSVFDGE+REGQVL IPQNFVV+ RASDRGFEWIAFK
Sbjct: 361 VNAHTVAYATRGSARVQVVDNYGQSVFDGEVREGQVLVIPQNFVVMTRASDRGFEWIAFK 420

Query: 421 TNDNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQEMRVLSPGRSQGRRD 478
           TNDNA+TNLLAGR SQMRLLPLGVLS+ YRISREEAQRLKYGQ EMR+ SPGRSQGRR+
Sbjct: 421 TNDNAITNLLAGRVSQMRLLPLGVLSNMYRISREEAQRLKYGQPEMRIFSPGRSQGRRE 476

BLAST of Lcy01g003380 vs. NCBI nr
Match: XP_022964523.1 (11S globulin subunit beta [Cucurbita moschata])

HSP 1 Score: 832.4 bits (2149), Expect = 2.0e-237
Identity = 410/480 (85.42%), Postives = 449/480 (93.54%), Query Frame = 0

Query: 1   MARPSFLSFLCLAVFIHACLAQIE-QIPWEFQGGEMRQQHRFQSSRACRLENLRAHEPVR 60
           MAR S  +FLCLAV I+ CL+QIE Q PWEFQG E+ QQHR+QS RACRLENLRA EPVR
Sbjct: 1   MARSSLFTFLCLAVLINGCLSQIEQQSPWEFQGSEVWQQHRYQSPRACRLENLRAQEPVR 60

Query: 61  RAEAEAGFTEVWDENNAEFQCAGVNMVRHTIRPRGMLQPGYSNAPKLIFVVRGSGLRGVA 120
           RAEAEAGFTEVWD++N EFQCAGVNM+RHTIRP+G+L PG+SNAPKL+FV +G G+RG+A
Sbjct: 61  RAEAEAGFTEVWDQDNDEFQCAGVNMIRHTIRPKGLLLPGFSNAPKLVFVAQGFGIRGIA 120

Query: 121 IPGCPETYQTDMRRSESAGSAFRDQHQKIREFREGDLLVLPAGVSHWIYNRGQSDLILIV 180
           IPGC ETYQTD+RRS+SAGSAFRDQHQKIR FREGDLLV+PAGVSHW+YNRGQSDL+LIV
Sbjct: 121 IPGCAETYQTDLRRSQSAGSAFRDQHQKIRPFREGDLLVVPAGVSHWMYNRGQSDLVLIV 180

Query: 181 FADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSASEKSGNVFSGFADELLE 240
           FADTRNVANQIDPYLRKF+LAGRPEQ+ERGVEEWER  R+GS+ EKSGN+FSGFADE LE
Sbjct: 181 FADTRNVANQIDPYLRKFYLAGRPEQVERGVEEWERSSRKGSSGEKSGNLFSGFADEFLE 240

Query: 241 EAFQIDSGLVRRLKGEDDERDRIVQVEEDFEVLMPEKDEQERQRGRYI--ESESDNGLEE 300
           EAFQID GLVR+LKGEDDERDRIVQV+EDFEVL+PEKDE+ER RGRYI  ESES+NGLEE
Sbjct: 241 EAFQIDGGLVRKLKGEDDERDRIVQVDEDFEVLLPEKDEEERSRGRYIESESESENGLEE 300

Query: 301 TICTLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSAERGVLYSNALVAPHY 360
           TICTLRLKHNIGRSERADVFNPRGGRISTANYH LP LRQVRLSAERGVLYSNA+VAPHY
Sbjct: 301 TICTLRLKHNIGRSERADVFNPRGGRISTANYHTLPVLRQVRLSAERGVLYSNAMVAPHY 360

Query: 361 TVNSHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFVVIKRASDRGFEWIAF 420
           TVNSH+V+YATRGSARVQVVDN+GQSVFDGE+REGQVL IPQNFV IKRASDRGFEWIAF
Sbjct: 361 TVNSHSVMYATRGSARVQVVDNFGQSVFDGEVREGQVLMIPQNFVAIKRASDRGFEWIAF 420

Query: 421 KTNDNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQEMRVLSPGRSQGRRD 478
           KTNDNA+TNLLAGR SQMR+LPLGVLS+ YRISREEAQRLKYGQQEMRVLSPGRSQGRR+
Sbjct: 421 KTNDNAITNLLAGRVSQMRMLPLGVLSNMYRISREEAQRLKYGQQEMRVLSPGRSQGRRE 480

BLAST of Lcy01g003380 vs. NCBI nr
Match: XP_023515280.1 (11S globulin subunit beta [Cucurbita pepo subsp. pepo] >KAG7026167.1 hypothetical protein SDJN02_12666, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 832.0 bits (2148), Expect = 2.6e-237
Identity = 410/480 (85.42%), Postives = 449/480 (93.54%), Query Frame = 0

Query: 1   MARPSFLSFLCLAVFIHACLAQIE-QIPWEFQGGEMRQQHRFQSSRACRLENLRAHEPVR 60
           MAR S  +FLCLAVFI+ CL+QIE Q PWEFQG E  QQHR+QS RACRLENLRA EPVR
Sbjct: 1   MARSSLFTFLCLAVFINGCLSQIEQQSPWEFQGSEEWQQHRYQSPRACRLENLRAQEPVR 60

Query: 61  RAEAEAGFTEVWDENNAEFQCAGVNMVRHTIRPRGMLQPGYSNAPKLIFVVRGSGLRGVA 120
           RAEAEAGFTEVWD++N EFQCAGVNM+RHTIRP+G+L PG+SNAPKL+FV +G G+RG+A
Sbjct: 61  RAEAEAGFTEVWDQDNDEFQCAGVNMIRHTIRPKGLLLPGFSNAPKLVFVAQGFGIRGIA 120

Query: 121 IPGCPETYQTDMRRSESAGSAFRDQHQKIREFREGDLLVLPAGVSHWIYNRGQSDLILIV 180
           IPGC ETYQTD+RRS+SAGSAFRDQHQKIR FREGDLLV+PAGVSHW+YNRGQSDL+LIV
Sbjct: 121 IPGCAETYQTDLRRSQSAGSAFRDQHQKIRPFREGDLLVVPAGVSHWMYNRGQSDLVLIV 180

Query: 181 FADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSASEKSGNVFSGFADELLE 240
           FADTRNVANQIDPYLRKF+LAGRPEQ+ERGVEEWER  R+GS+ EKSGN+FSGFADE LE
Sbjct: 181 FADTRNVANQIDPYLRKFYLAGRPEQVERGVEEWERSSRKGSSGEKSGNLFSGFADEFLE 240

Query: 241 EAFQIDSGLVRRLKGEDDERDRIVQVEEDFEVLMPEKDEQERQRGRYI--ESESDNGLEE 300
           EAFQID GLVR+LKGEDDERDRIVQV+EDFEVL+PEKDE+ER RGRYI  ESES+NGLEE
Sbjct: 241 EAFQIDGGLVRKLKGEDDERDRIVQVDEDFEVLLPEKDEEERSRGRYIESESESENGLEE 300

Query: 301 TICTLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSAERGVLYSNALVAPHY 360
           TICTLRLKHNIGRSERADVFNPRGGRISTANYH LP LRQVRLSAERGVLYSNA+VAPHY
Sbjct: 301 TICTLRLKHNIGRSERADVFNPRGGRISTANYHTLPVLRQVRLSAERGVLYSNAMVAPHY 360

Query: 361 TVNSHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFVVIKRASDRGFEWIAF 420
           TVNSH+V+YATRGSARVQVVDN+GQSVFDGE++EGQVL IPQNFVVIKRASDRGFEWIAF
Sbjct: 361 TVNSHSVMYATRGSARVQVVDNFGQSVFDGEVQEGQVLMIPQNFVVIKRASDRGFEWIAF 420

Query: 421 KTNDNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQEMRVLSPGRSQGRRD 478
           KTNDNA+TNLLAGR SQMR+LPLGVLS+ YRISREEAQRLKYGQQEMRV SPGRSQGRR+
Sbjct: 421 KTNDNAITNLLAGRVSQMRMLPLGVLSNMYRISREEAQRLKYGQQEMRVFSPGRSQGRRE 480

BLAST of Lcy01g003380 vs. NCBI nr
Match: XP_023000493.1 (11S globulin subunit beta [Cucurbita maxima])

HSP 1 Score: 830.5 bits (2144), Expect = 7.4e-237
Identity = 409/480 (85.21%), Postives = 450/480 (93.75%), Query Frame = 0

Query: 1   MARPSFLSFLCLAVFIHACLAQIE-QIPWEFQGGEMRQQHRFQSSRACRLENLRAHEPVR 60
           MAR S  +FLCLAVFI+ CL+QIE Q PWEFQG E+ QQHR+QS RACRLENLRA +PVR
Sbjct: 1   MARSSLFTFLCLAVFINGCLSQIEQQSPWEFQGSEVWQQHRYQSPRACRLENLRAQDPVR 60

Query: 61  RAEAEAGFTEVWDENNAEFQCAGVNMVRHTIRPRGMLQPGYSNAPKLIFVVRGSGLRGVA 120
           RAEAEAGFTEVWD++N EFQCAGVNM+RHTIRP+G+L PG+SNAPKLIFV +G G+RG+A
Sbjct: 61  RAEAEAGFTEVWDQDNDEFQCAGVNMIRHTIRPKGLLLPGFSNAPKLIFVAQGFGIRGIA 120

Query: 121 IPGCPETYQTDMRRSESAGSAFRDQHQKIREFREGDLLVLPAGVSHWIYNRGQSDLILIV 180
           IPGC ETYQTD+RRS+SAGSAF+DQHQKIR FREGDLLV+PAGVSHW+YNRGQSDL+LIV
Sbjct: 121 IPGCAETYQTDLRRSQSAGSAFKDQHQKIRPFREGDLLVVPAGVSHWMYNRGQSDLVLIV 180

Query: 181 FADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSASEKSGNVFSGFADELLE 240
           FADTRNVANQIDPYLRKF+LAGRPEQ+ERGVEEWER  R+GS+ EKSGN+FSGFADE LE
Sbjct: 181 FADTRNVANQIDPYLRKFYLAGRPEQVERGVEEWERSSRKGSSGEKSGNIFSGFADEFLE 240

Query: 241 EAFQIDSGLVRRLKGEDDERDRIVQVEEDFEVLMPEKDEQERQRGRYI--ESESDNGLEE 300
           EAFQID GLVR+LKGEDDERDRIVQV+EDFEVL+PEKDE+ER RGRYI  ESES+NGLEE
Sbjct: 241 EAFQIDGGLVRKLKGEDDERDRIVQVDEDFEVLLPEKDEEERSRGRYIESESESENGLEE 300

Query: 301 TICTLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSAERGVLYSNALVAPHY 360
           TICTLRLK NIGRSERADVFNPRGGRISTANYH LP LRQVRLSAERGVLYSNA+VAPHY
Sbjct: 301 TICTLRLKQNIGRSERADVFNPRGGRISTANYHTLPILRQVRLSAERGVLYSNAMVAPHY 360

Query: 361 TVNSHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFVVIKRASDRGFEWIAF 420
           TVNSH+V+YATRG+ARVQVVDN+GQSVFDGE+REGQVL IPQNFVVIKRASDRGFEWIAF
Sbjct: 361 TVNSHSVMYATRGNARVQVVDNFGQSVFDGEVREGQVLMIPQNFVVIKRASDRGFEWIAF 420

Query: 421 KTNDNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQEMRVLSPGRSQGRRD 478
           KTNDNA+TNLLAGR SQMR+LPLGVLS+ YRISREEAQRLKYGQQEMRVLSPGRSQGRR+
Sbjct: 421 KTNDNAITNLLAGRVSQMRMLPLGVLSNMYRISREEAQRLKYGQQEMRVLSPGRSQGRRE 480

BLAST of Lcy01g003380 vs. NCBI nr
Match: KAG6593835.1 (hypothetical protein SDJN03_13311, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 824.3 bits (2128), Expect = 5.3e-235
Identity = 406/474 (85.65%), Postives = 443/474 (93.46%), Query Frame = 0

Query: 1   MARPSFLSFLCLAVFIHACLAQIE-QIPWEFQGGEMRQQHRFQSSRACRLENLRAHEPVR 60
           MAR S  +FLCLAVFI+ CL+QIE Q PWEFQG E  QQHR+QS RACRLENLRA EPVR
Sbjct: 1   MARSSLFTFLCLAVFINGCLSQIEQQSPWEFQGSEEWQQHRYQSPRACRLENLRAQEPVR 60

Query: 61  RAEAEAGFTEVWDENNAEFQCAGVNMVRHTIRPRGMLQPGYSNAPKLIFVVRGSGLRGVA 120
           RAEAEAGFTEVWD++N EFQCAGVNM+RHTIRP+G+L PG+SNAPKL+FV +G G+RG+A
Sbjct: 61  RAEAEAGFTEVWDQDNDEFQCAGVNMIRHTIRPKGLLLPGFSNAPKLVFVAQGFGIRGIA 120

Query: 121 IPGCPETYQTDMRRSESAGSAFRDQHQKIREFREGDLLVLPAGVSHWIYNRGQSDLILIV 180
           IPGC ETYQTD+RRS+SAGSAFRDQHQKIR FREGDLLV+PAGVSHW+YNRGQSDL+LIV
Sbjct: 121 IPGCAETYQTDLRRSQSAGSAFRDQHQKIRPFREGDLLVVPAGVSHWMYNRGQSDLVLIV 180

Query: 181 FADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSASEKSGNVFSGFADELLE 240
           FADTRNVANQIDPYLRKF+LAGRPEQ+ERGVEEWER  R+GS+ EKSGN+FSGFADE LE
Sbjct: 181 FADTRNVANQIDPYLRKFYLAGRPEQVERGVEEWERSSRKGSSGEKSGNLFSGFADEFLE 240

Query: 241 EAFQIDSGLVRRLKGEDDERDRIVQVEEDFEVLMPEKDEQERQRGRYI--ESESDNGLEE 300
           EAFQID GLVR+LKGEDDERDRIVQV+EDFEVL+PEKDE+ER RGRYI  ESES+NGLEE
Sbjct: 241 EAFQIDGGLVRKLKGEDDERDRIVQVDEDFEVLLPEKDEEERSRGRYIESESESENGLEE 300

Query: 301 TICTLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSAERGVLYSNALVAPHY 360
           TICTLRLKHNIGRSERADVFNPRGGRISTANYH LP LRQVRLSAERGVLYSNA+VAPHY
Sbjct: 301 TICTLRLKHNIGRSERADVFNPRGGRISTANYHTLPVLRQVRLSAERGVLYSNAMVAPHY 360

Query: 361 TVNSHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFVVIKRASDRGFEWIAF 420
           TVNSH+V+YATRGSARVQVVDN+GQSVFDGE+REGQVL IPQNFVVIKRASDRGFEWIAF
Sbjct: 361 TVNSHSVMYATRGSARVQVVDNFGQSVFDGEVREGQVLMIPQNFVVIKRASDRGFEWIAF 420

Query: 421 KTNDNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQEMRVLSPGR 472
           KTNDNA+TNLLAGR SQMR+LPLGVLS+ YRISREEAQRLKYGQQEMRV SPGR
Sbjct: 421 KTNDNAITNLLAGRVSQMRMLPLGVLSNMYRISREEAQRLKYGQQEMRVFSPGR 474

BLAST of Lcy01g003380 vs. NCBI nr
Match: P13744.1 (RecName: Full=11S globulin subunit beta; Contains: RecName: Full=11S globulin gamma chain; AltName: Full=11S globulin acidic chain; Contains: RecName: Full=11S globulin delta chain; AltName: Full=11S globulin basic chain; Flags: Precursor [Cucurbita maxima] >pir|FWPU1B| 11S globulin beta subunit precursor - cucurbita cv. Kurokawa Amakuri [Cucurbita hybrid cultivar] >AAA33110.1 11-S globulin beta-subunit precursor [Cucurbita pepo])

HSP 1 Score: 823.9 bits (2127), Expect = 7.0e-235
Identity = 407/480 (84.79%), Postives = 448/480 (93.33%), Query Frame = 0

Query: 1   MARPSFLSFLCLAVFIHACLAQIE-QIPWEFQGGEMRQQHRFQSSRACRLENLRAHEPVR 60
           MAR S  +FLCLAVFI+ CL+QIE Q PWEFQG E+ QQHR+QS RACRLENLRA +PVR
Sbjct: 1   MARSSLFTFLCLAVFINGCLSQIEQQSPWEFQGSEVWQQHRYQSPRACRLENLRAQDPVR 60

Query: 61  RAEAEAGFTEVWDENNAEFQCAGVNMVRHTIRPRGMLQPGYSNAPKLIFVVRGSGLRGVA 120
           RAEAEA FTEVWD++N EFQCAGVNM+RHTIRP+G+L PG+SNAPKLIFV +G G+RG+A
Sbjct: 61  RAEAEAIFTEVWDQDNDEFQCAGVNMIRHTIRPKGLLLPGFSNAPKLIFVAQGFGIRGIA 120

Query: 121 IPGCPETYQTDMRRSESAGSAFRDQHQKIREFREGDLLVLPAGVSHWIYNRGQSDLILIV 180
           IPGC ETYQTD+RRS+SAGSAF+DQHQKIR FREGDLLV+PAGVSHW+YNRGQSDL+LIV
Sbjct: 121 IPGCAETYQTDLRRSQSAGSAFKDQHQKIRPFREGDLLVVPAGVSHWMYNRGQSDLVLIV 180

Query: 181 FADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSASEKSGNVFSGFADELLE 240
           FADTRNVANQIDPYLRKF+LAGRPEQ+ERGVEEWER  R+GS+ EKSGN+FSGFADE LE
Sbjct: 181 FADTRNVANQIDPYLRKFYLAGRPEQVERGVEEWERSSRKGSSGEKSGNIFSGFADEFLE 240

Query: 241 EAFQIDSGLVRRLKGEDDERDRIVQVEEDFEVLMPEKDEQERQRGRYI--ESESDNGLEE 300
           EAFQID GLVR+LKGEDDERDRIVQV+EDFEVL+PEKDE+ER RGRYI  ESES+NGLEE
Sbjct: 241 EAFQIDGGLVRKLKGEDDERDRIVQVDEDFEVLLPEKDEEERSRGRYIESESESENGLEE 300

Query: 301 TICTLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSAERGVLYSNALVAPHY 360
           TICTLRLK NIGRS RADVFNPRGGRISTANYH LP LRQVRLSAERGVLYSNA+VAPHY
Sbjct: 301 TICTLRLKQNIGRSVRADVFNPRGGRISTANYHTLPILRQVRLSAERGVLYSNAMVAPHY 360

Query: 361 TVNSHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFVVIKRASDRGFEWIAF 420
           TVNSH+V+YATRG+ARVQVVDN+GQSVFDGE+REGQVL IPQNFVVIKRASDRGFEWIAF
Sbjct: 361 TVNSHSVMYATRGNARVQVVDNFGQSVFDGEVREGQVLMIPQNFVVIKRASDRGFEWIAF 420

Query: 421 KTNDNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQEMRVLSPGRSQGRRD 478
           KTNDNA+TNLLAGR SQMR+LPLGVLS+ YRISREEAQRLKYGQQEMRVLSPGRSQGRR+
Sbjct: 421 KTNDNAITNLLAGRVSQMRMLPLGVLSNMYRISREEAQRLKYGQQEMRVLSPGRSQGRRE 480

BLAST of Lcy01g003380 vs. TAIR 10
Match: AT1G03890.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 334.3 bits (856), Expect = 1.6e-91
Identity = 181/449 (40.31%), Postives = 254/449 (56.57%), Query Frame = 0

Query: 30  FQGGEMRQQHRFQSSRACRLENLRAHEPVRRAEAEAGFTEVWDENNAEFQCAGVNMVRHT 89
           F G E RQ+       AC    + +  P +  + EAG  EVWD  + E +CAGV + R T
Sbjct: 20  FHGAEARQREA-PFPNACHFSQINSLAPAQATKFEAGQMEVWDHMSPELRCAGVTVARIT 79

Query: 90  IRPRGMLQPGYSNAPKLIFVVRGSGLRGVAIPGCPETYQTDMRRSESAGSA-----FRDQ 149
           ++P  +  P + + P L +VV+G G+ G    GCPET+      S   G       F D 
Sbjct: 80  LQPNSIFLPAFFSPPALAYVVQGEGVMGTIASGCPETFAEVEGSSGRGGGGDPGRRFEDM 139

Query: 150 HQKIREFREGDLLVLPAGVSHWIYNRGQSDLILIVFADTRNVANQIDPYLRKFHLAGRPE 209
           HQK+  FR GD+    AGVS W YNRG SD ++++  D  N  NQ+D   R F LAG   
Sbjct: 140 HQKLENFRRGDVFASLAGVSQWWYNRGDSDAVIVIVLDVTNRENQLDQVPRMFQLAGSRT 199

Query: 210 QIERGVEEWERRGRRGSASEKSGNVFSGFADELLEEAFQIDSGLVRRLKGEDDERDRIVQ 269
           Q E     W              N FSGF   ++ EAF+I+    ++L+ + D R  I++
Sbjct: 200 QEEEQPLTW----------PSGNNAFSGFDPNIIAEAFKINIETAKQLQNQKDNRGNIIR 259

Query: 270 VEEDFEVLMPEKDEQERQRGRYIESESDNGLEETICTLRLKHNIGRSERADVFNPRGGRI 329
                  ++P   E +       +    NG+EET CT ++  NI   ER+D F+ R GRI
Sbjct: 260 ANGPLHFVIPPPREWQ-------QDGIANGIEETYCTAKIHENIDDPERSDHFSTRAGRI 319

Query: 330 STANYHNLPALRQVRLSAERGVLYSNALVAPHYTVNSHTVVYATRGSARVQVVDNYGQSV 389
           ST N  NLP LR VRL+A RG LYS  +V P +T N+HTV+Y T G A++QVVD+ GQSV
Sbjct: 320 STLNSLNLPVLRLVRLNALRGYLYSGGMVLPQWTANAHTVLYVTGGQAKIQVVDDNGQSV 379

Query: 390 FDGELREGQVLTIPQNFVVIKRASDRGFEWIAFKTNDNAVTNLLAGRASQMRLLPLGVLS 449
           F+ ++ +GQ++ IPQ F V K A + GFEWI+FKTNDNA  N L+G+ S +R +P+ V+ 
Sbjct: 380 FNEQVGQGQIIVIPQGFAVSKTAGETGFEWISFKTNDNAYINTLSGQTSYLRAVPVDVIK 439

Query: 450 SSYRISREEAQRLKYGQQE-MRVLSPGRS 473
           +SY ++ EEA+R+K+ QQE M  ++P  S
Sbjct: 440 ASYGVNEEEAKRIKFSQQETMLSMTPSSS 450

BLAST of Lcy01g003380 vs. TAIR 10
Match: AT1G03880.1 (cruciferin 2 )

HSP 1 Score: 329.3 bits (843), Expect = 5.0e-90
Identity = 181/421 (42.99%), Postives = 246/421 (58.43%), Query Frame = 0

Query: 47  CRLENLRAHEPVRRAEAEAGFTEVWDENNAEFQCAGVNMVRHTIRPRGMLQPGYSNAPKL 106
           C+L+ L A EP +  ++E G  EVWD +  + +C+G    R  I P+G+  P + NA KL
Sbjct: 30  CQLDQLNALEPSQIIKSEGGRIEVWDHHAPQLRCSGFAFERFVIEPQGLFLPTFLNAGKL 89

Query: 107 IFVVRGSGLRGVAIPGCPETYQTDMRRSESAG----SAFRDQHQKIREFREGDLLVLPAG 166
            FVV G GL G  IPGC ET+       E  G      FRD HQK+   R GD +  P+G
Sbjct: 90  TFVVHGRGLMGRVIPGCAETFMESPVFGEGQGQGQSQGFRDMHQKVEHLRCGDTIATPSG 149

Query: 167 VSHWIYNRGQSDLILIVFADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSA 226
           V+ W YN G   LIL+  AD  +  NQ+D  LR F +AG   Q     +EW     +G  
Sbjct: 150 VAQWFYNNGNEPLILVAAADLASNQNQLDRNLRPFLIAGNNPQ----GQEW----LQGRK 209

Query: 227 SEKSGNVFSGFADELLEEAFQIDSGLVRRLKGEDDERDRIVQVEEDFEVLMPEKDEQERQ 286
            +K  N+F+GFA E+L +AF+I+    ++L+ + D R  IV+V   F V+ P      R 
Sbjct: 210 QQKQNNIFNGFAPEILAQAFKINVETAQQLQNQQDNRGNIVKVNGPFGVIRP---PLRRG 269

Query: 287 RGRYIESESDNGLEETICTLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSA 346
            G     E  NGLEET+CT+R   N+     ADV+ P  G IST N +NLP LR +RLSA
Sbjct: 270 EGGQQPHEIANGLEETLCTMRCTENLDDPSDADVYKPSLGYISTLNSYNLPILRLLRLSA 329

Query: 347 ERGVLYSNALVAPHYTVNSHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFV 406
            RG +  NA+V P + VN++  +Y T G A +Q+V++ G+ VFD E+  GQ+L +PQ F 
Sbjct: 330 LRGSIRKNAMVLPQWNVNANAALYVTNGKAHIQMVNDNGERVFDQEISSGQLLVVPQGFS 389

Query: 407 VIKRASDRGFEWIAFKTNDNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQ 464
           V+K A    FEWI FKTN+NA  N LAGR S MR LPL V+++ Y+IS EEA+R+K+   
Sbjct: 390 VMKHAIGEQFEWIEFKTNENAQVNTLAGRTSVMRGLPLEVITNGYQISPEEAKRVKFSTI 439

BLAST of Lcy01g003380 vs. TAIR 10
Match: AT5G44120.3 (RmlC-like cupins superfamily protein )

HSP 1 Score: 325.9 bits (834), Expect = 5.6e-89
Identity = 188/478 (39.33%), Postives = 273/478 (57.11%), Query Frame = 0

Query: 1   MARPSFLSFLCLAVFI--HACLAQIEQIPWEFQGGEMRQQHRFQSSRACRLENLRAHEPV 60
           MAR S L   CL + I  H   AQ        QG     Q   Q    C+L+ L A EP 
Sbjct: 1   MARVSSLLSFCLTLLILFHGYAAQ--------QG-----QQGQQFPNECQLDQLNALEPS 60

Query: 61  RRAEAEAGFTEVWDENNAEFQCAGVNMVRHTIRPRGMLQPGYSNAPKLIFVVRGSGLRGV 120
              ++EAG  EVWD +  + +C+GV+  R+ I  +G+  P + N  KL FV +G GL G 
Sbjct: 61  HVLKSEAGRIEVWDHHAPQLRCSGVSFARYIIESKGLYLPSFFNTAKLSFVAKGRGLMGK 120

Query: 121 AIPGCPETYQTDMR---RSESAGSA--FRDQHQKIREFREGDLLVLPAGVSHWIYNRGQS 180
            IPGC ET+Q       R E  G +  FRD HQK+   R GD +    GV+ W YN GQ 
Sbjct: 121 VIPGCAETFQDSSEFQPRFEGQGQSQRFRDMHQKVEHIRSGDTIATTPGVAQWFYNDGQE 180

Query: 181 DLILIVFADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSASEKSGNVFSGF 240
            L+++   D  +  NQ+D   R F+LAG   Q     + W     +G   +   N+F+GF
Sbjct: 181 PLVIVSVFDLASHQNQLDRNPRPFYLAGNNPQ----GQVW----LQGREQQPQKNIFNGF 240

Query: 241 ADELLEEAFQIDSGLVRRLKGEDDERDRIVQVEEDFEVLMP--------EKDEQERQRGR 300
             E++ +A +ID    ++L+ +DD R  IV+V+  F V+ P        E++E+E + GR
Sbjct: 241 GPEVIAQALKIDLQTAQQLQNQDDNRGNIVRVQGPFGVIRPPLRGQRPQEEEEEEGRHGR 300

Query: 301 YIESESDNGLEETICTLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSAERG 360
           +      NGLEETIC+ R   N+    RADV+ P+ G IST N ++LP LR +RLSA RG
Sbjct: 301 H-----GNGLEETICSARCTDNLDDPSRADVYKPQLGYISTLNSYDLPILRFIRLSALRG 360

Query: 361 VLYSNALVAPHYTVNSHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFVVIK 420
            +  NA+V P +  N++ ++Y T G A++Q+V++ G  VFDG++ +GQ++ +PQ F V+K
Sbjct: 361 SIRQNAMVLPQWNANANAILYVTDGEAQIQIVNDNGNRVFDGQVSQGQLIAVPQGFSVVK 420

Query: 421 RASDRGFEWIAFKTNDNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQE 464
           RA+   F+W+ FKTN NA  N LAGR S +R LPL V+++ ++IS EEA+R+K+   E
Sbjct: 421 RATSNRFQWVEFKTNANAQINTLAGRTSVLRGLPLEVITNGFQISPEEARRVKFNTLE 452

BLAST of Lcy01g003380 vs. TAIR 10
Match: AT4G28520.1 (cruciferin 3 )

HSP 1 Score: 300.8 bits (769), Expect = 1.9e-81
Identity = 167/492 (33.94%), Postives = 260/492 (52.85%), Query Frame = 0

Query: 42  QSSRACRLENLRAHEPVRRAEAEAGFTEVWDENNAEFQCAGVNMVRHTIRPRGMLQPGYS 101
           Q    C L+NL   +     ++EAG  E WD N+ + +C GV++ R+ I   G+  P + 
Sbjct: 32  QLQNECNLDNLDVLQATETIKSEAGQIEYWDHNHPQLRCVGVSVARYVIEQGGLYLPTFF 91

Query: 102 NAPKLIFVVRGSGLRGVAIPGCPETYQTDM------------------------------ 161
            +PK+ +VV+G+G+ G  +PGC ET+                                  
Sbjct: 92  TSPKISYVVQGTGISGRVVPGCAETFMDSQPMQGQQQGQPWQGRQGQQGQPWEGQGQQGQ 151

Query: 162 ----------------------------RRSESAGSAFRDQHQKIREFREGDLLVLPAGV 221
                                        + +     FRD HQK+   R GD+     G 
Sbjct: 152 QGRQGQPWEGQGQQGQQGRQGQQGQPWEGQGQQGQQGFRDMHQKVEHVRRGDVFANTPGS 211

Query: 222 SHWIYNRGQSDLILIVFADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSAS 281
           +HWIYN G+  L++I   D  N  NQ+D   R FHLAG  +Q   G  + +         
Sbjct: 212 AHWIYNSGEQPLVIIALLDIANYQNQLDRNPRVFHLAGNNQQGGFGGSQQQ--------- 271

Query: 282 EKSGNVFSGFADELLEEAFQIDSGLVRRLKGEDDERDRIVQVEEDFEVLMPE-KDEQERQ 341
           ++  N++SGF  +++ +A +ID  L ++L+ + D R  IV+V+  F+V+ P  +   E +
Sbjct: 272 QEQKNLWSGFDAQVIAQALKIDVQLAQQLQNQQDSRGNIVRVKGPFQVVRPPLRQPYESE 331

Query: 342 RGRYIESESDNGLEETICTLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSA 401
             R+  S   NGLEETIC++R   NI    RADV+ P  GR+++ N + LP L  VRLSA
Sbjct: 332 EWRHPRSPQGNGLEETICSMRSHENIDDPARADVYKPSLGRVTSVNSYTLPILEYVRLSA 391

Query: 402 ERGVLYSNALVAPHYTVNSHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFV 461
            RGVL  NA+V P Y +N++ ++Y T G  R+QVV++ GQ+V D ++++GQ++ IPQ F 
Sbjct: 392 TRGVLQGNAMVLPKYNMNANEILYCTGGQGRIQVVNDNGQNVLDQQVQKGQLVVIPQGFA 451

Query: 462 VIKRASDRGFEWIAFKTNDNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQ 474
            + ++    FEWI+FKTN+NA+ + LAGR S +R LPL V+S+ ++IS EEA+++K+   
Sbjct: 452 YVVQSHGNKFEWISFKTNENAMISTLAGRTSLLRALPLEVISNGFQISPEEARKIKFNTL 511

BLAST of Lcy01g003380 vs. TAIR 10
Match: AT5G44120.2 (RmlC-like cupins superfamily protein )

HSP 1 Score: 265.4 bits (677), Expect = 8.9e-71
Identity = 145/360 (40.28%), Postives = 215/360 (59.72%), Query Frame = 0

Query: 117 GVAIPGCPETYQTDMR---RSESAGSA--FRDQHQKIREFREGDLLVLPAGVSHWIYNRG 176
           G  IPGC ET+Q       R E  G +  FRD HQK+   R GD +    GV+ W YN G
Sbjct: 2   GKVIPGCAETFQDSSEFQPRFEGQGQSQRFRDMHQKVEHIRSGDTIATTPGVAQWFYNDG 61

Query: 177 QSDLILIVFADTRNVANQIDPYLRKFHLAGRPEQIERGVEEWERRGRRGSASEKSGNVFS 236
           Q  L+++   D  +  NQ+D   R F+LAG   Q     + W     +G   +   N+F+
Sbjct: 62  QEPLVIVSVFDLASHQNQLDRNPRPFYLAGNNPQ----GQVW----LQGREQQPQKNIFN 121

Query: 237 GFADELLEEAFQIDSGLVRRLKGEDDERDRIVQVEEDFEVLMP--------EKDEQERQR 296
           GF  E++ +A +ID    ++L+ +DD R  IV+V+  F V+ P        E++E+E + 
Sbjct: 122 GFGPEVIAQALKIDLQTAQQLQNQDDNRGNIVRVQGPFGVIRPPLRGQRPQEEEEEEGRH 181

Query: 297 GRYIESESDNGLEETICTLRLKHNIGRSERADVFNPRGGRISTANYHNLPALRQVRLSAE 356
           GR+      NGLEETIC+ R   N+    RADV+ P+ G IST N ++LP LR +RLSA 
Sbjct: 182 GRH-----GNGLEETICSARCTDNLDDPSRADVYKPQLGYISTLNSYDLPILRFIRLSAL 241

Query: 357 RGVLYSNALVAPHYTVNSHTVVYATRGSARVQVVDNYGQSVFDGELREGQVLTIPQNFVV 416
           RG +  NA+V P +  N++ ++Y T G A++Q+V++ G  VFDG++ +GQ++ +PQ F V
Sbjct: 242 RGSIRQNAMVLPQWNANANAILYVTDGEAQIQIVNDNGNRVFDGQVSQGQLIAVPQGFSV 301

Query: 417 IKRASDRGFEWIAFKTNDNAVTNLLAGRASQMRLLPLGVLSSSYRISREEAQRLKYGQQE 464
           +KRA+   F+W+ FKTN NA  N LAGR S +R LPL V+++ ++IS EEA+R+K+   E
Sbjct: 302 VKRATSNRFQWVEFKTNANAQINTLAGRTSVLRGLPLEVITNGFQISPEEARRVKFNTLE 348

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P137449.1e-23884.7911S globulin subunit beta OS=Cucurbita maxima OX=3661 PE=1 SV=1[more]
A0A1L6K3715.5e-12650.1011S globulin OS=Juglans nigra OX=16719 PE=1 SV=1[more]
B5KVH44.7e-12549.5111S globulin seed storage protein 1 OS=Carya illinoinensis OX=32201 PE=1 SV=1[more]
Q2TPW58.0e-12549.6111S globulin seed storage protein Jug r 4 OS=Juglans regia OX=51240 PE=1 SV=1[more]
Q8GZP62.0e-12046.2311S globulin seed storage protein Ana o 2.0101 (Fragment) OS=Anacardium occident... [more]
Match NameE-valueIdentityDescription
A0A6J1HL289.5e-23885.4211S globulin subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111464525 PE=3 SV=... [more]
A0A6J1KMS53.6e-23785.2111S globulin subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111494748 PE=3 SV=1[more]
A0A6J1KVP51.2e-23284.9111S globulin subunit beta-like OS=Cucurbita maxima OX=3661 GN=LOC111499130 PE=3 ... [more]
A0A6J1H6094.3e-23084.2811S globulin subunit beta-like OS=Cucurbita moschata OX=3662 GN=LOC111460434 PE=... [more]
A0A1S3CIE11.1e-22080.1711S globulin subunit beta-like OS=Cucumis melo OX=3656 GN=LOC103500763 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022964523.12.0e-23785.4211S globulin subunit beta [Cucurbita moschata][more]
XP_023515280.12.6e-23785.4211S globulin subunit beta [Cucurbita pepo subsp. pepo] >KAG7026167.1 hypothetica... [more]
XP_023000493.17.4e-23785.2111S globulin subunit beta [Cucurbita maxima][more]
KAG6593835.15.3e-23585.65hypothetical protein SDJN03_13311, partial [Cucurbita argyrosperma subsp. sorori... [more]
P13744.17.0e-23584.79RecName: Full=11S globulin subunit beta; Contains: RecName: Full=11S globulin ga... [more]
Match NameE-valueIdentityDescription
AT1G03890.11.6e-9140.31RmlC-like cupins superfamily protein [more]
AT1G03880.15.0e-9042.99cruciferin 2 [more]
AT5G44120.35.6e-8939.33RmlC-like cupins superfamily protein [more]
AT4G28520.11.9e-8133.94cruciferin 3 [more]
AT5G44120.28.9e-7140.28RmlC-like cupins superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR00604411-S seed storage protein, plantPRINTSPR0043911SGLOBULINcoord: 305..322
score: 47.69
coord: 328..348
score: 51.53
coord: 374..390
score: 59.54
coord: 392..407
score: 58.91
coord: 410..428
score: 49.29
coord: 432..449
score: 37.96
IPR006045Cupin 1SMARTSM00835Cupin_1_3coord: 306..455
e-value: 3.2E-54
score: 196.1
coord: 50..250
e-value: 1.6E-33
score: 127.4
IPR006045Cupin 1PFAMPF00190Cupin_1coord: 312..454
e-value: 2.0E-32
score: 111.8
coord: 54..204
e-value: 6.2E-27
score: 94.0
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 309..477
e-value: 2.3E-73
score: 247.0
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 46..308
e-value: 2.2E-110
score: 370.1
NoneNo IPR availablePANTHERPTHR31189OS03G0336100 PROTEIN-RELATEDcoord: 8..472
NoneNo IPR availablePANTHERPTHR31189:SF48LEGUMIN Bcoord: 8..472
NoneNo IPR availableCDDcd02242cupin_11S_legumin_Ncoord: 49..267
e-value: 4.87894E-98
score: 291.796
NoneNo IPR availableCDDcd02243cupin_11S_legumin_Ccoord: 315..469
e-value: 2.30322E-84
score: 254.706
IPR02237911-S seed storage protein, conserved sitePROSITEPS0030511S_SEED_STORAGEcoord: 293..315
IPR011051RmlC-like cupin domain superfamilySUPERFAMILY51182RmlC-like cupinscoord: 37..463

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy01g003380.1Lcy01g003380.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding
molecular_function GO:0045735 nutrient reservoir activity