Lcy01g002960 (gene) Sponge gourd (P93075) v1

BLAST of Lcy01g002960 vs. ExPASy Swiss-Prot
Match: Q9SS87 (Protein SIEVE ELEMENT OCCLUSION B OS=Arabidopsis thaliana OX=3702 GN=SEOB PE=1 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 7.4e-55
Identity = 185/712 (25.98%), Postives = 317/712 (44.52%), Query Frame = 0

Query: 32  SDDVIMNQMYTKHNDDDTIKIDVNNYILFIESVIKTADEIALALHGAQGSKGHFVFSDD- 91
           SD+ +M ++  + +  D  ++ V   +  +E ++   D   L       S       D  
Sbjct: 38  SDESMMLKLIQQTHSPDAREVQVRGLLSLVEDIL---DRATLDSEDTNASMLPLPTEDKL 97

Query: 92  -SSKFPSSIDPPICTLHEISRELACKAPGIETAHKTTLDILSKLTRYPWDAKAVLIFAAF 151
             S   S +D     +  ++ E+A K+     +H+ T+ +   L+ + WD K VL  AAF
Sbjct: 98  MQSSMMSVLDSVSYAIDRVACEIAYKSLTGSDSHEITMSVFEHLSSFQWDGKLVLTLAAF 157

Query: 152 ATNYGVMWHLDNYSHTDDPLAKSLAMIKRVALL-RKELDSLKYGQVFFDSNSLIYSCLKA 211
           A NYG  W L  + ++ + LAKSLAM+K V +  R  L+S+  G      N LI      
Sbjct: 158 ALNYGEFWLLVQF-YSKNQLAKSLAMLKLVPVQNRVTLESVSQG-----LNDLIREMKSV 217

Query: 212 IKYMNEIKN-SSKYDTKEVPELSAALRQIPLVSYWIIHTLVASSTELHCYLS-GAE---- 271
              + E+     +Y T +VP+LS  L  IP+  YW I +++A  ++++   + G E    
Sbjct: 218 TACVVELSELPDRYITPDVPQLSRILSTIPIAVYWTIRSVIACISQINMITAMGHEMMNT 277

Query: 272 ----GQTQKYLNELTEKFQTLLYTLEKHLQFIREQQGEVELYRWLVDQTDHFPTDITLFL 331
                +T    N+L      L  TL    + I E+Q   E  + L    D    D    L
Sbjct: 278 QMDLWETSMLANKLKNIHDHLAETLRLCYRHI-EKQRSSESLKVLHSLFDTTHIDNMKIL 337

Query: 332 SKLIDGRHKARPLINCSTQMEEYIEDFLKDKNLILVVSKSVEISKEDLESLNLIYSELKK 391
           + L+  +    PL +  T+ + ++ D L+ K ++L++S  + I +++L     IY+E ++
Sbjct: 338 TALVHPKPHITPLQDGLTKRKVHL-DVLRRKTVLLLIS-DLNILQDELSIFEQIYTESRR 397

Query: 392 G---------NNYEIVWIPVISDPPNDGD-----EEAYEYLKSKMKWYAM--PFTTKIAG 451
                       YE+VW+PV+ DP  D +     ++ +E L+  M WY++  P   +   
Sbjct: 398 NLVGVDGKSHMPYEVVWVPVV-DPIEDFERSPILQKKFEDLRDPMPWYSVDSPKLIERHV 457

Query: 452 VRFLEEKWELREDLLVVVLNTQSKIEFTNAIHLIRVWEKEAIPLTYERAKALLKKNWID- 511
           V F+  +W      ++VV++ Q      NA+H+I +W  EA P T  R + L ++     
Sbjct: 458 VEFMRGRWHFMNKPILVVIDPQGNEASLNALHMIWIWGTEAFPFTRSREEELWRRETFSL 517

Query: 512 STVVKFSDEPRLNSLVMINQERNIIFYGGHNPRWIKNFEETADAIKRDPLTREEGISFEI 571
           + +V   D    N    I  +  I  YGG +  WI+ F   A A  +D       ++ E+
Sbjct: 518 NLIVDGIDSVIFN---WIKPDNYIFLYGGDDLDWIRRFTMAAKATAKD-----SNVNLEM 577

Query: 572 LPVGLNNKGEQ--------------------DPAVMFRFWMAQRSFFILKHQLQGSTASE 631
             VG  N   +                    +PA+M+ FW    S    K QL  +   +
Sbjct: 578 AYVGKRNHSHREQIRRISEVIRSENLSHSWAEPALMWFFWTRLESMLYSKIQLGKADDHD 637

Query: 632 D----ISRLISYENENGWAIIAKGPSVVFVGGGDLIIKAMEEFKLWKKNIRRVGFSGSFK 686
           D    I +++SY+   GWA+++KGP +V +  G +        + WK ++   G++ +  
Sbjct: 638 DVMQGIKKILSYDKLGGWALLSKGPEIVMIAHGAIERTMSVYDRTWKTHVPTKGYTKAMS 697

BLAST of Lcy01g002960 vs. ExPASy Swiss-Prot
Match: Q93XX2 (Protein SIEVE ELEMENT OCCLUSION A OS=Arabidopsis thaliana OX=3702 GN=SEOA PE=1 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 4.2e-42
Identity = 184/758 (24.27%), Postives = 320/758 (42.22%), Query Frame = 0

Query: 6   PKTPAAQLAISLSQQVDKEERSLRHLSDDVIMNQMYTKHNDDDTIKIDVNNYILFIESVI 65
           P +   +      Q   +  R +  LSDD +M     K +  D I  DV + +  +  + 
Sbjct: 111 PNSLGGRFGPGKKQAFHRNGRPMFSLSDDRVMADRVLKTHSPDMIFFDVTSLLSVVNDIF 170

Query: 66  KTADEIALALHGAQGSKGHFVFSD--DSSKFPSSIDPPICTLHEISRELACK-------- 125
           K+     +    +   K   VF D  D + F +  D     + +IS E+ CK        
Sbjct: 171 KS----HVPSIDSSAPKPSLVFKDYADHTSFETFAD----LIDQISCEIDCKCLHGGESH 230

Query: 126 -----APGIETAHKTTLDILSKLTRYPWDAKAVLIFAAFATNYGVMWHLDNYSHTDDPLA 185
                   +++ + TT  +LS +++Y WDAK VL+ +A A  YGV   L   +H  + L 
Sbjct: 231 GMMTSGLHLDSRNTTTFSVLSLVSKYRWDAKLVLVLSALAVKYGVFLLLAE-THATNQLT 290

Query: 186 KSLAMIKRVALLRKELDSLKYGQVFFDSNSLIYSCLKAIKYMNEIKN--SSKYDTKEVP- 245
           KSLA+IK++              +F   N+L     K    M ++ +  ++  D  ++P 
Sbjct: 291 KSLALIKQLP------------SIFSRQNALHQRLDKTRILMQDMVDLTTTIIDIYQLPP 350

Query: 246 -ELSAALR-QIPLVSYWIIHTLVASSTELHCYLSGAEGQTQ---------KYLNELTEKF 305
             ++AA    IP   YWI+  ++   +    ++SGA G  Q           ++E +E+ 
Sbjct: 351 NHITAAFTDHIPTAVYWIVRCVLICVS----HISGASGFKQDQIMSFMEVSEIHENSERL 410

Query: 306 QT----LLYTLEKHLQFIREQQGEVELYRWLVDQTDHFPTDITLFLSKLI---------D 365
           +     LL   +K    I E   E E    +   T     D+   L +L+          
Sbjct: 411 RKINAYLLEQFKKSKMTIEEGIIEEEYQELIQTFTTIIHVDVVPPLLRLLRPIDFLYHGA 470

Query: 366 GRHKARPLINCSTQMEEYIEDFLKDKNLILVVSKSVEISKEDLESLNLIYSELKKGNNYE 425
           G  K R  IN  TQ           K+++L++S    I KE L  L  +Y+E  +  ++E
Sbjct: 471 GVSKRRVGINVLTQ-----------KHVLLLISDLENIEKE-LYILESLYTEAWQ-QSFE 530

Query: 426 IVWIPVISDPPNDGDEEAYEYLKSKMKWYAM--PFTTKIAGVRFLEEKWELREDLLVVVL 485
           I+W+PV  D   + D+  +E L   M+WY +  P   + A +RF+ E W  +   ++V L
Sbjct: 531 ILWVPV-QDFWTEADDAKFEALHMNMRWYVLGEPRKLRRAAIRFVREWWGFKNRPILVAL 590

Query: 486 NTQSKIEFTNAIHLIRVWEKEAIPLTYERAKAL-LKKNWIDSTVVKFSDEPRLNSLVMIN 545
           + + ++  TNA  ++ +W+  A P T  R + L  ++ W    ++  +D   LN LV   
Sbjct: 591 DPKGQVMSTNAFPMVWIWQPFAHPFTTARERDLWSEQEWNLEFLIDGTDPHSLNQLV--- 650

Query: 546 QERNIIFYGGHNPRWIKNFEETADAIKRDPLTREEGISFEILPVGLNN-KGEQDPAV--- 605
             + I  YGG + +WIKNF     ++ R+ + +   I  E++ VG  N K    P +   
Sbjct: 651 DGKYICLYGGEDMQWIKNF----TSLWRN-VAKAANIQLEMVYVGKRNPKNGIQPIINTI 710

Query: 606 -----------MFRFWM---------AQRSFFILKHQLQG---------STASEDISRLI 665
                      +F+ W            +   +  H ++G             +++  ++
Sbjct: 711 REENLSHTLPDLFQIWFFWTRVESMWESKQRMLKAHGIKGREGFKEEEKDLVLQEVVAML 770

Query: 666 SYENE-NGWAIIAKGPSVVFVGGGDLIIKAMEEFKLWKKNIRRVGFSGSFKDYFDELTAT 685
            Y  E +GW +++K   ++    G+L  + + EF  W+ NI   GF  +  D+   +   
Sbjct: 771 GYGGEGDGWGLVSKASDMMVRAKGNLFSRGLAEFNEWEVNIPTKGFLTALNDHL-LMRLP 820

BLAST of Lcy01g002960 vs. ExPASy Swiss-Prot
Match: Q9FXE2 (Protein SIEVE ELEMENT OCCLUSION C OS=Arabidopsis thaliana OX=3702 GN=SEOC PE=4 SV=2)

HSP 1 Score: 102.8 bits (255), Expect = 1.6e-20
Identity = 146/696 (20.98%), Postives = 284/696 (40.80%), Query Frame = 0

Query: 26  RSLRHLSDDVIMNQMYTKHNDDDTIKIDVNNYILFIESVIK--TADEIALALHGAQGSKG 85
           R +  L++D+I+ Q+   H D D   +D    +  +E+++     ++++  L        
Sbjct: 5   RDISALNEDIIVEQLLRSH-DPDGRWLDSEMLLQEVETILSFVLQNDVSRPLLTENCITT 64

Query: 86  HFVFSDDSSKFPSSIDPPICTLHEISRELACKAPGIETAHKTTLDILSKLTRYPWDAKAV 145
             VF D     P +I         IS ++ C   G     K T+ +   L  Y WDAKAV
Sbjct: 65  IEVF-DSKETLPYAI-------FRISVQMLCPCTGENEIRKRTMVLFDLLKEYRWDAKAV 124

Query: 146 LIFAAFATNYGVMWHLDNYSHTDDPLAKSLAMIKRVALLRKE----LDSLKYGQVFFDSN 205
           L+    A  YG +  L  +    DP+A S+A + ++ + R +    L+SL    +   + 
Sbjct: 125 LVLGVLAATYGGLL-LPVHLAICDPVAASIAKLNQLPIERTKFRPWLESL---NLLIKAM 184

Query: 206 SLIYSCLKAIKYMNEIKNSSKYDTKEVPELSAALRQIPLVSYWIIHTLVASSTELHCY-- 265
             +  C+  IK+       +K D   + E    L  I L +Y ++ + +    ++  +  
Sbjct: 185 VDVTKCI--IKFEKIPFKQAKLDNNILGE---TLSNIYLTTYRVVKSALTCMQQIPYFKQ 244

Query: 266 -------------LSGAEGQTQKYLNELTEKFQTLLYTLEKHLQFIREQQGEV--ELYRW 325
                        LS    +    L+ L  +   +   L K ++    Q  E   +  R 
Sbjct: 245 TQQAKKSRKTAAELSIESRRAAGELSSLGYQLLNIHTRLNKQVEDCSTQIEEEINQRLRN 304

Query: 326 LVDQTDHFPTDITLFLSKLIDGRHKARPLINCSTQMEEYIEDFLKDKNLILVVSK-SVEI 385
           +  +T     D+   L  L D      PL   S Q+   I + ++DK  +L++SK  VE 
Sbjct: 305 INIETHQDNQDVLHLLFSLQDD----LPLQQYSRQIS--ITE-VQDKVTLLLLSKPPVEP 364

Query: 386 SKEDLESLNLIYSELKKGNNYEIVWIPVISDPP-NDGDEEAYEYLKSKMKWYAM--PFTT 445
               L+ L    S      NYEI+W+P+ S     D ++E +++  + + W ++  P+  
Sbjct: 365 LFFLLQQLYDHPSNTNTEQNYEIIWVPIPSSQKWTDEEKEIFDFYSNSLPWISVRQPWLM 424

Query: 446 KIAGVRFLEEKWELRE-DLLVVVLNTQSKIEFTNAIHLIRVWEKEAIPLTYERAKALLKK 505
               + F +++W  ++ + ++VV+++  +    NA+ ++ +W  +A P +  R   L K+
Sbjct: 425 SSTILNFFKQEWHYKDNEAMLVVIDSNGRFVNMNAMDMVLIWGVKAYPFSVSREDELWKE 484

Query: 506 NWIDSTVVKFSDEPRLNSLVMINQERNIIFYGGHNPRWIKNFEETADAIKRDPLTREEGI 565
           +        +S    L+ +    + R I  +G  N  WI  F   A  I        + +
Sbjct: 485 H-------GWSINLLLDGIHPTFEGREICIFGSENLDWIDEFVSLARKI--------QNL 544

Query: 566 SFEILPVGLNNKGEQD-----------PAVMFRFWMAQRSF------FILKHQLQGSTAS 625
            F++  + L+N+   +           P +   FW+   S        I+    +     
Sbjct: 545 GFQLELIYLSNQRRDERAMEESSILFSPTLQQLFWLRLESIERSKLKRIVIEPSKPDRVF 604

Query: 626 EDISRLI--SYENENGWAIIAKGPSVVFVGGGDLIIKAMEEFKLWKKNIRRVGFSGSFKD 673
           E++  L+   Y    GW II  G +   V  G+ + + M +   W +  + +GF+ +   
Sbjct: 605 EEVRNLLDFDYGKHRGWGIIGNGSTAETV-DGEKMTERMRKIVRWGEYAKGLGFTEAI-- 654

BLAST of Lcy01g002960 vs. ExPASy TrEMBL
Match: A0A6J1H3Z4 (protein SIEVE ELEMENT OCCLUSION B-like OS=Cucurbita moschata OX=3662 GN=LOC111459939 PE=4 SV=1)

HSP 1 Score: 1143.3 bits (2956), Expect = 0.0e+00
Identity = 533/691 (77.13%), Postives = 626/691 (90.59%), Query Frame = 0

Query: 1   MSTARPKTPAAQLAISLSQQVDKEERSLRHLSDDVIMNQMYTKHNDDDTIKIDVNNYILF 60
           MS  RPK+ AA LA++LSQQ DKEE  L+HLSDDVI N+++    D++  KID++NY+LF
Sbjct: 1   MSLLRPKSSAAALALALSQQTDKEEPGLKHLSDDVITNRIFDFTTDEEMTKIDLDNYVLF 60

Query: 61  IESVIKTADEIALALHGAQGSKGHFVFSDDSSKFPSSIDPPICTLHEISRELACKAPGIE 120
           IE+V+K +D+I +A H AQGSKGHFV ++D+  +PS IDPPICTLH+IS E+ACK PG+E
Sbjct: 61  IENVLKASDQICVASHWAQGSKGHFVLAEDTMNYPSPIDPPICTLHQISSEMACKPPGVE 120

Query: 121 TAHKTTLDILSKLTRYPWDAKAVLIFAAFATNYGVMWHLDNYSHTDDPLAKSLAMIKRVA 180
           T HKTTLDILSKLTRYPW+AKAVLIF AF TNYGV+WHLDN+SH+ DPLAKSLAMIKRV 
Sbjct: 121 TVHKTTLDILSKLTRYPWEAKAVLIFTAFVTNYGVLWHLDNHSHS-DPLAKSLAMIKRVG 180

Query: 181 LLRKELDSLKYGQVFFDSNSLIYSCLKAIKYMNEIKNSSKYDTKEVPELSAALRQIPLVS 240
           LLRKEL+S+KYGQVFF+ NS+IY+CLKA+KY+NE K+ SKYDTK+VPELSAALRQIPLVS
Sbjct: 181 LLRKELESVKYGQVFFNQNSMIYNCLKAVKYINEFKSLSKYDTKDVPELSAALRQIPLVS 240

Query: 241 YWIIHTLVASSTELHCYLSGAEGQTQKYLNELTEKFQTLLYTLEKHLQFIREQQGEVELY 300
           YWIIH LVASS ELHCYLSG EGQTQKYLNE+TEK  +L+ TLE H+QFIREQQ EVELY
Sbjct: 241 YWIIHILVASSIELHCYLSGVEGQTQKYLNEMTEKVSSLVLTLENHMQFIREQQEEVELY 300

Query: 301 RWLVDQTDHFPTDITLFLSKLIDGRHKARPLINCSTQMEEYIEDFLKDKNLILVVSKSVE 360
           RWLVDQTDHFPTD+TLFL+KLI+G+HKARPLINCSTQ+EE+IE+F+K+K LIL+VS+S+ 
Sbjct: 301 RWLVDQTDHFPTDVTLFLAKLINGKHKARPLINCSTQLEEHIEEFMKEKKLILIVSRSLN 360

Query: 361 ISKEDLESLNLIYSELKKGNNYEIVWIPVISDPPNDGDEEAYEYLKSKMKWYAMPFTTKI 420
            S+EDLE L  +Y E+KK N +E+VWIPVISDPPNDGDEEAYE LKS+MKW+ +PF  KI
Sbjct: 361 FSREDLEILQSVYKEVKKENKFEMVWIPVISDPPNDGDEEAYEALKSEMKWFVVPFAAKI 420

Query: 421 AGVRFLEEKWELREDLLVVVLNTQSKIEFTNAIHLIRVWEKEAIPLTYERAKALLKKNWI 480
           AGVRFLEE+WELREDLL+VVL+TQSK+EF+NAIHL RVWEKEAIP +Y+R KAL+KKNWI
Sbjct: 421 AGVRFLEERWELREDLLLVVLDTQSKVEFSNAIHLTRVWEKEAIPFSYDRVKALMKKNWI 480

Query: 481 DSTVVKFSDEPRLNSLVMINQERNIIFYGGHNPRWIKNFEETADAIKRDPLTREEGISFE 540
           DSTV+K++D+PRL SLV+INQERN+IFYGGHNPRW+KNFE++A+A+KRDP+TREEGI+FE
Sbjct: 481 DSTVLKYTDQPRLKSLVVINQERNVIFYGGHNPRWVKNFEDSAEAMKRDPVTREEGITFE 540

Query: 541 ILPVGLNNKGEQDPAVMFRFWMAQRSFFILKHQLQGSTASEDISRLISYENENGWAIIAK 600
           ++PVG NNKGE DPAVMF FWMAQRS+FILKHQL GSTASEDISRLISYE E GWAII K
Sbjct: 541 LVPVGRNNKGEHDPAVMFNFWMAQRSYFILKHQLHGSTASEDISRLISYETEKGWAIITK 600

Query: 601 GPSVVFVGGGDLIIKAMEEFKLWKKNIRRVGFSGSFKDYFDELTATSLHCTHVNIIGFSG 660
           GP VVF+GGGDLI+KA++EF++WKKN+RRVGFSGSFKDYFDELTA+SLHCTHVNIIGFSG
Sbjct: 601 GPIVVFIGGGDLILKAIDEFQVWKKNLRRVGFSGSFKDYFDELTASSLHCTHVNIIGFSG 660

Query: 661 WIPLIITCPVCRRYMGSGIRFTCCHGGPDVL 692
           WIPLIITCPVCRRYMGSGIRFTCCHGGPDVL
Sbjct: 661 WIPLIITCPVCRRYMGSGIRFTCCHGGPDVL 690

BLAST of Lcy01g002960 vs. ExPASy TrEMBL
Match: A0A6J1KWJ8 (protein SIEVE ELEMENT OCCLUSION B-like OS=Cucurbita maxima OX=3661 GN=LOC111499359 PE=4 SV=1)

HSP 1 Score: 1133.6 bits (2931), Expect = 0.0e+00
Identity = 531/692 (76.73%), Postives = 628/692 (90.75%), Query Frame = 0

Query: 1   MSTARPKTPAAQLAISLSQQVDKEERSLRHLSDDVIMNQMYTKHNDDDTIKIDVNNYILF 60
           MS  R K+ A  LA++LSQQ DKEE  L+HLSDDVI N+++    D++ +KID++NY++F
Sbjct: 1   MSLLRSKSSATALALALSQQTDKEEPDLKHLSDDVITNRIFNFTTDEEMMKIDLDNYVVF 60

Query: 61  IESVIKTADEIALALHGAQGSKGHFVFSDDSSKFPSS-IDPPICTLHEISRELACKAPGI 120
           IE+V+K +D+I +A H AQGSKG+FV ++D+  +PSS IDPPICTLH+IS E+ACK PG+
Sbjct: 61  IENVLKASDQICVASHWAQGSKGNFVLAEDTMNYPSSPIDPPICTLHQISSEMACKPPGV 120

Query: 121 ETAHKTTLDILSKLTRYPWDAKAVLIFAAFATNYGVMWHLDNYSHTDDPLAKSLAMIKRV 180
           ET HKTTLDILSKLTRYPW+AKA+LIFAAF TNYGV+WHLDN+SH+ DPLAKSLAMIKRV
Sbjct: 121 ETVHKTTLDILSKLTRYPWEAKAMLIFAAFMTNYGVLWHLDNHSHS-DPLAKSLAMIKRV 180

Query: 181 ALLRKELDSLKYGQVFFDSNSLIYSCLKAIKYMNEIKNSSKYDTKEVPELSAALRQIPLV 240
            LLRKEL+S+KYGQVFF+ NS+IY+CLKA+KY+ E K+ SKYDTK+VPELSAALR+IPLV
Sbjct: 181 GLLRKELESVKYGQVFFNQNSMIYNCLKAVKYIIEFKSLSKYDTKDVPELSAALRRIPLV 240

Query: 241 SYWIIHTLVASSTELHCYLSGAEGQTQKYLNELTEKFQTLLYTLEKHLQFIREQQGEVEL 300
           SYWIIH LVASS ELHCYLSG EGQTQKYLNE+TEK  TL+ TLE H+QFIREQQ EVEL
Sbjct: 241 SYWIIHILVASSIELHCYLSGVEGQTQKYLNEMTEKVSTLVLTLENHMQFIREQQEEVEL 300

Query: 301 YRWLVDQTDHFPTDITLFLSKLIDGRHKARPLINCSTQMEEYIEDFLKDKNLILVVSKSV 360
           YRWLVDQTDHFPTD+TLFL+KLI+G+HKARPLINCSTQ+EE+IE+F+K+K LIL+VS+S+
Sbjct: 301 YRWLVDQTDHFPTDVTLFLAKLINGKHKARPLINCSTQLEEHIEEFMKEKKLILIVSRSL 360

Query: 361 EISKEDLESLNLIYSELKKGNNYEIVWIPVISDPPNDGDEEAYEYLKSKMKWYAMPFTTK 420
             S+EDLE L  IY E+KK N +E+VWIPVISDPPNDGDEEAYE LKS+MKW+ +PF TK
Sbjct: 361 NFSREDLEILQSIYKEVKKENKFEMVWIPVISDPPNDGDEEAYEALKSEMKWFVVPFATK 420

Query: 421 IAGVRFLEEKWELREDLLVVVLNTQSKIEFTNAIHLIRVWEKEAIPLTYERAKALLKKNW 480
           IAGVRFLEE+WELREDLL+VVL+TQSK+EF+NAIHL RVWEKEAIPL+Y+R K L+KKNW
Sbjct: 421 IAGVRFLEERWELREDLLLVVLDTQSKVEFSNAIHLTRVWEKEAIPLSYDRVKVLMKKNW 480

Query: 481 IDSTVVKFSDEPRLNSLVMINQERNIIFYGGHNPRWIKNFEETADAIKRDPLTREEGISF 540
           IDSTV+KF+D+PRL SLV+INQERN+IFYGGHNPRW+KNFEE+A+A+KRDP+TREEGI+F
Sbjct: 481 IDSTVLKFTDQPRLKSLVVINQERNVIFYGGHNPRWVKNFEESAEAMKRDPVTREEGITF 540

Query: 541 EILPVGLNNKGEQDPAVMFRFWMAQRSFFILKHQLQGSTASEDISRLISYENENGWAIIA 600
           E++PVG NNKGE DPAVMF FWMAQRS+FILKHQL GSTASEDISR+ISYE E GWAII 
Sbjct: 541 ELVPVGRNNKGEHDPAVMFNFWMAQRSYFILKHQLHGSTASEDISRMISYETEKGWAIIT 600

Query: 601 KGPSVVFVGGGDLIIKAMEEFKLWKKNIRRVGFSGSFKDYFDELTATSLHCTHVNIIGFS 660
           KGP+VVF+GGGDLI+KA++EF++WKKN+R+VGFSGSFKDYFDELTA+SLHCTHVNIIGFS
Sbjct: 601 KGPTVVFIGGGDLILKAIDEFQIWKKNLRQVGFSGSFKDYFDELTASSLHCTHVNIIGFS 660

Query: 661 GWIPLIITCPVCRRYMGSGIRFTCCHGGPDVL 692
           GWIPLIITCPVCRRYMGSGIRFTCCHGGPDVL
Sbjct: 661 GWIPLIITCPVCRRYMGSGIRFTCCHGGPDVL 691

BLAST of Lcy01g002960 vs. ExPASy TrEMBL
Match: A0A5A7UTQ8 (Protein SIEVE ELEMENT OCCLUSION B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1503G00130 PE=4 SV=1)

HSP 1 Score: 1118.2 bits (2891), Expect = 0.0e+00
Identity = 533/692 (77.02%), Postives = 622/692 (89.88%), Query Frame = 0

Query: 1   MSTARPKTPAAQLAISLSQQVDK-EERSLRHLSDDVIMNQMYTKHNDDDTIKIDVNNYIL 60
           MS  RPKT A  LA++L Q++DK EE+SL+HLSDD I N+++T ++D +T+KID+++YIL
Sbjct: 1   MSIFRPKTSATALALALPQEIDKEEEKSLKHLSDDFITNRIFTINSDAETMKIDIDSYIL 60

Query: 61  FIESVIKTADEIALALHGAQGSKGHFVFSDDSSKFPSSIDPPICTLHEISRELACKAPGI 120
           FIESVIK++D+IA+A H A+GSKGHF  +D++ K+P+ IDPPICTLH+IS E+ CK PG 
Sbjct: 61  FIESVIKSSDKIAVASHWAKGSKGHFELADETLKYPTQIDPPICTLHQISTEMICKDPGA 120

Query: 121 ETAHKTTLDILSKLTRYPWDAKAVLIFAAFATNYGVMWHLDNYSHTDDPLAKSLAMIKRV 180
           ETAH+TTLDIL KL +Y WDAKAVLIF AFAT YGV+WHLDNYSH+ D LAKSLA IKRV
Sbjct: 121 ETAHQTTLDILQKLAKYSWDAKAVLIFTAFATTYGVLWHLDNYSHS-DTLAKSLATIKRV 180

Query: 181 ALLRKELDSLKYGQVFFDSNSLIYSCLKAIKYMNEIKNSSKYDTKEVPELSAALRQIPLV 240
           ALLRKELDS+KYGQVFF+ NS+IY+C+KAIKY+NE +N SKYDTK+VPELSAALRQIPLV
Sbjct: 181 ALLRKELDSVKYGQVFFNPNSMIYNCMKAIKYINEFRNLSKYDTKDVPELSAALRQIPLV 240

Query: 241 SYWIIHTLVASSTELHCYLSGAEGQTQKYLNELTEKFQTLLYTLEKHLQFIREQQGEVEL 300
           SYWIIHTLVASS ELHCYLSG +GQT KYLNEL+EK +++L TLE HLQ IREQ  EVEL
Sbjct: 241 SYWIIHTLVASSIELHCYLSGVQGQTHKYLNELSEKSESVLLTLENHLQLIREQIEEVEL 300

Query: 301 YRWLVDQTDHFPTDITLFLSKLIDGRHKARPLINCSTQMEEYIEDFLKDKNLILVVSKSV 360
           YRWLVDQTDHFPTDITLFLSKLIDG+HKARPLINCSTQ+EEYIEDFLK+K LIL+VSK +
Sbjct: 301 YRWLVDQTDHFPTDITLFLSKLIDGKHKARPLINCSTQLEEYIEDFLKEKKLILIVSKRL 360

Query: 361 EISKEDLESLNLIYSELKKGNNYEIVWIPVISDPPNDGDEEAYEYLKSKMKWYAMPFTTK 420
           +IS EDLE L  IY+E+KK N +E+VWIPVI DPP DGDEE YEYLKS MKWY +PF TK
Sbjct: 361 DISTEDLEILYSIYNEVKKENKFEMVWIPVIPDPPMDGDEEGYEYLKSVMKWYMVPFNTK 420

Query: 421 IAGVRFLEEKWELREDLLVVVLNTQSKIEFTNAIHLIRVWEKEAIPLTYERAKALLKKNW 480
           IAG+RFLEE+WELRED+L+VVLNTQSK+EF+N IHL R+WEKEA+P TY+RAKALLKKNW
Sbjct: 421 IAGMRFLEERWELREDILMVVLNTQSKVEFSNGIHLARIWEKEALPFTYDRAKALLKKNW 480

Query: 481 IDSTVVKFSDEPRLNSLVMINQERNIIFYGGHNPRWIKNFEETADAIKRDPLTREEGISF 540
           IDSTVVKF+D+PRL SLV+INQERN+IFYGGHN RWIK FEE+A+ +KRDP TREEGI+F
Sbjct: 481 IDSTVVKFTDQPRLRSLVVINQERNVIFYGGHNLRWIKKFEESAEVMKRDPTTREEGITF 540

Query: 541 EILPVGLNNKGEQDPAVMFRFWMAQRSFFILKHQLQGSTASEDISRLISYENENGWAIIA 600
           E+ PVGL NKGEQDPA+MFRFWMAQRS+FILKHQLQGSTA+EDISRLISYE E+GWAII 
Sbjct: 541 ELAPVGL-NKGEQDPALMFRFWMAQRSYFILKHQLQGSTATEDISRLISYETEDGWAIIT 600

Query: 601 KGPSVVFVGGGDLIIKAMEEFKLWKKNIRRVGFSGSFKDYFDELTATSLHCTHVNIIGFS 660
           KGP+VVFV GGDLI+KAM+EF LWKKN+RR+GFSGSFK++FDELTATSLHCT+VN+IGFS
Sbjct: 601 KGPTVVFVAGGDLILKAMDEFHLWKKNMRRLGFSGSFKEHFDELTATSLHCTNVNLIGFS 660

Query: 661 GWIPLIITCPVCRRYMGSGIRFTCCHGGPDVL 692
           GWIPL +TCP+CRRYMGSGIRFTCCHGGPDVL
Sbjct: 661 GWIPLFLTCPMCRRYMGSGIRFTCCHGGPDVL 690

BLAST of Lcy01g002960 vs. ExPASy TrEMBL
Match: A0A6J1C9F2 (protein SIEVE ELEMENT OCCLUSION B-like OS=Momordica charantia OX=3673 GN=LOC111009603 PE=4 SV=1)

HSP 1 Score: 1102.0 bits (2849), Expect = 0.0e+00
Identity = 535/692 (77.31%), Postives = 614/692 (88.73%), Query Frame = 0

Query: 1   MSTARPKTPAAQLAISLSQQVDKEERSLRHLSDDVIMNQMYTKHNDDDTIKIDVNNYILF 60
           MST RPKTPAA LA++ +     EERSL++ SDDVI + +YTKH+DD+ +KID+NNYI F
Sbjct: 1   MSTPRPKTPAAPLALAAN-----EERSLKYFSDDVITSHIYTKHSDDEAMKIDLNNYISF 60

Query: 61  IESVIKTADEIA-LALHGAQGSKGHFVFSDDSSKFPSSIDPPICTLHEISRELACKAPGI 120
           IE+V+KTAD+IA   L   QGSKGHFVFSDD  K+PSSIDPP+CTLH+IS+ELACKAPGI
Sbjct: 61  IENVVKTADQIAETHLGRVQGSKGHFVFSDDPLKYPSSIDPPLCTLHQISKELACKAPGI 120

Query: 121 ETAHKTTLDILSKLTRYPWDAKAVLIFAAFATNYGVMWHLDNYSHTDDPLAKSLAMIKRV 180
           ETAHKTTLDIL+KL  YPW+AKA L FAAFAT+YGV+WHLD+YSH+ DPLAKSLAMIKRV
Sbjct: 121 ETAHKTTLDILNKLISYPWEAKAALTFAAFATDYGVIWHLDHYSHS-DPLAKSLAMIKRV 180

Query: 181 ALLRKELDSLKYGQVFFDSNSLIYSCLKAIKYMNEIKNSSKYDTKEVPELSAALRQIPLV 240
           A L+KEL+S KY  V    NSLIYSCL+AIK +NE+KN SKYDTKEVPELSAALRQIPLV
Sbjct: 181 AWLKKELESFKYRHV----NSLIYSCLQAIKNINELKNISKYDTKEVPELSAALRQIPLV 240

Query: 241 SYWIIHTLVASSTELHCYLSGAEGQTQKYLNELTEKFQTLLYTLEKHLQFIREQQGEVEL 300
           SYWIIHTLVASSTELH YLSGAEGQT KYLNEL EKF++LL+T EK LQFIREQQ EVEL
Sbjct: 241 SYWIIHTLVASSTELHSYLSGAEGQTLKYLNELDEKFRSLLFTFEKQLQFIREQQEEVEL 300

Query: 301 YRWLVDQTDHFPTDITLFLSKLIDGRHKARPLINCSTQMEEYIEDFLKDKNLILVVSKSV 360
           Y+ LV QTDHFPTD+TLFLSKLIDG+HK +PLINCSTQ+EE+IE+FLK+KNLILVVSK +
Sbjct: 301 YKSLVGQTDHFPTDVTLFLSKLIDGKHKPQPLINCSTQLEEHIEEFLKEKNLILVVSKGL 360

Query: 361 EISKEDLESLNLIYSELKKGNNYEIVWIPVISDPPNDGDEEAYEYLKSKMKWYAMPFTTK 420
           + S EDLE L++IY ELK+ N YEIVW+PVISDPPN+GDEEAYE LKSKMKWYA+PFTTK
Sbjct: 361 KFSMEDLEILHVIYDELKRENKYEIVWVPVISDPPNEGDEEAYESLKSKMKWYALPFTTK 420

Query: 421 IAGVRFLEEKWELREDLLVVVLNTQSKIEFTNAIHLIRVWEKEAIPLTYERAKALLKKNW 480
           +AGVRFLEEKW+LREDL+VVVL+++SKIEFTNA+HL RVW++EAIP TYERAKALL+KNW
Sbjct: 421 VAGVRFLEEKWQLREDLMVVVLDSKSKIEFTNAVHLTRVWKREAIPFTYERAKALLRKNW 480

Query: 481 IDSTVVKFSDEPRLNSLVMINQERNIIFYGGHNPRWIKNFEETADAIKRDPLTREEGISF 540
           IDSTVVKFSD+PRL S   INQERNIIFYGGHNP W++ FEETA+AIKRDPL REEG +F
Sbjct: 481 IDSTVVKFSDQPRLRS--WINQERNIIFYGGHNPNWVRKFEETAEAIKRDPLAREEGATF 540

Query: 541 EILPVGLNNKGEQDPAVMFRFWMAQRSFFILKHQLQGSTASEDISRLISYENENGWAIIA 600
           E++P+G N+KGE DPAVMFRFWM QRSFF +KHQL GS+ASED+S+LISYENENGWAII 
Sbjct: 541 EMVPIGRNDKGEHDPAVMFRFWMVQRSFFNIKHQLHGSSASEDVSQLISYENENGWAIIV 600

Query: 601 KGPSVVFVGGGDLIIKAMEEFKLWKKNIRRVGFSGSFKDYFDELTATSLHCTHVNIIGFS 660
           KGP+V+FVGGGDLI+KAMEEF+ WKKN+R  GFS SFKDY+ ELTA S HCTHVNI+GFS
Sbjct: 601 KGPTVIFVGGGDLILKAMEEFQAWKKNLRWEGFSASFKDYYHELTAKSPHCTHVNILGFS 660

Query: 661 GWIPLIITCPVCRRYMGSGIRFTCCHGGPDVL 692
           GWIPLII CP+C RYMGSGIRFTCCHGGPDVL
Sbjct: 661 GWIPLIIVCPMCHRYMGSGIRFTCCHGGPDVL 680

BLAST of Lcy01g002960 vs. ExPASy TrEMBL
Match: A0A6J1EQ44 (protein SIEVE ELEMENT OCCLUSION B-like OS=Cucurbita moschata OX=3662 GN=LOC111436801 PE=4 SV=1)

HSP 1 Score: 1101.7 bits (2848), Expect = 0.0e+00
Identity = 521/687 (75.84%), Postives = 609/687 (88.65%), Query Frame = 0

Query: 5   RPKTPAAQLAISLSQQVDKEERSLRHLSDDVIMNQMYTKHNDDDTIKIDVNNYILFIESV 64
           RPK     LA++L Q+ DKE+ +L+HLSDDVIMN ++TKH DD+TIKID+N+YI FIE+V
Sbjct: 8   RPKISVVPLALALMQKTDKEQTNLKHLSDDVIMNHLFTKHGDDETIKIDLNDYISFIENV 67

Query: 65  IKTADEIALALHGAQGSKGHFVFSDDSSKFPSSIDPPICTLHEISRELACKAPGIETAHK 124
           IK++D+I+ A H AQGSK H   SDDS  + SSI+PP+CTLH+IS+++ CKA GI+ AHK
Sbjct: 68  IKSSDQISAASHWAQGSKVHVELSDDSFTYLSSIEPPVCTLHDISKQMGCKAAGIDIAHK 127

Query: 125 TTLDILSKLTRYPWDAKAVLIFAAFATNYGVMWHLDNYSHTDDPLAKSLAMIKRVALLRK 184
           TTLDILSKLT+Y W+AKAVLIFAAFA NYG +WHLDNYS + DPLAKSLAMIKRV  LRK
Sbjct: 128 TTLDILSKLTKYSWEAKAVLIFAAFAMNYGALWHLDNYSRS-DPLAKSLAMIKRVGSLRK 187

Query: 185 ELDSLKYGQVFFDSNSLIYSCLKAIKYMNEIKNSSKYDTKEVPELSAALRQIPLVSYWII 244
           ELDS++YGQVFF  NSLIYSCLKAIKYMNE KN SKYD KEVPELSAALR+IPLVSYWI+
Sbjct: 188 ELDSVQYGQVFFGPNSLIYSCLKAIKYMNEFKNLSKYDIKEVPELSAALRRIPLVSYWIV 247

Query: 245 HTLVASSTELHCYLSGAEGQTQKYLNELTEKFQTLLYTLEKHLQFIREQQGEVELYRWLV 304
           H LVASS +LHCYLSG EGQT KYLNEL+EK +++L TLEKH QFI++QQ EVELYRWLV
Sbjct: 248 HALVASSIDLHCYLSGTEGQTHKYLNELSEKKRSILVTLEKHHQFIQKQQEEVELYRWLV 307

Query: 305 DQTDHFPTDITLFLSKLIDGRHKARPLINCSTQMEEYIEDFLKDKNLILVVSKSVEISKE 364
           DQTDHFP DITLFLSKLI G+HKARPLINCST++EEYIEDFLK+KNLIL+VSK +++SKE
Sbjct: 308 DQTDHFPIDITLFLSKLIHGKHKARPLINCSTRLEEYIEDFLKEKNLILIVSKRLDVSKE 367

Query: 365 DLESLNLIYSELKKGNNYEIVWIPVISDPPNDGDEEAYEYLKSKMKWYAMPFTTKIAGVR 424
           DL+SLN++Y+E+KKGN +EIVW+PVISDPP +GDEEAYE L SKMKWYA+PF TK+AG+R
Sbjct: 368 DLDSLNVVYNEVKKGNKHEIVWVPVISDPPAEGDEEAYEALISKMKWYAVPFDTKVAGLR 427

Query: 425 FLEEKWELREDLLVVVLNTQSKIEFTNAIHLIRVWEKEAIPLTYERAKALLKKNWIDSTV 484
           FLEEKWE+REDLL+VVLNTQSKIEF NA+HL R+WEKEAIP TYERA ALLK++WIDSTV
Sbjct: 428 FLEEKWEVREDLLIVVLNTQSKIEFPNAVHLTRIWEKEAIPFTYERANALLKRSWIDSTV 487

Query: 485 VKFSDEPRLNSLVMINQERNIIFYGGHNPRWIKNFEETADAIKRDPLTREEGISFEILPV 544
           VKF+D+PRLNSL MIN+ER +IFYGGHN  WIK FE+TA+AIKRD + REEGI+FE++P+
Sbjct: 488 VKFTDQPRLNSLAMINKERTMIFYGGHNQGWIKRFEDTAEAIKRDLMLREEGITFELVPL 547

Query: 545 GLNNKGEQDPAVMFRFWMAQRSFFILKHQLQGSTASEDISRLISYENENGWAIIAKGPSV 604
           GLN KGE DP +M RFW AQRSFFILKHQLQGSTA+EDISRLISYENE GWAI+ KG +V
Sbjct: 548 GLNQKGEPDPTIMSRFWTAQRSFFILKHQLQGSTATEDISRLISYENEKGWAIVTKGQTV 607

Query: 605 VFVGGGDLIIKAMEEFKLWKKNIRRVGFSGSFKDYFDELTATSLHCTHVNIIGFSGWIPL 664
           V VGGGDLI+KA+EEF+ WKKN+RRVGFSGSFKDYFDELT+ SL CTHVNI+G+SGWIPL
Sbjct: 608 VMVGGGDLIVKALEEFQTWKKNLRRVGFSGSFKDYFDELTSKSLECTHVNIMGYSGWIPL 667

Query: 665 IITCPVCRRYMGSGIRFTCCHGGPDVL 692
            + CPVCRRYMGSGIRFTCCHG P+VL
Sbjct: 668 TVMCPVCRRYMGSGIRFTCCHGRPNVL 693

BLAST of Lcy01g002960 vs. NCBI nr
Match: XP_023548586.1 (protein SIEVE ELEMENT OCCLUSION B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1144.0 bits (2958), Expect = 0.0e+00
Identity = 533/691 (77.13%), Postives = 626/691 (90.59%), Query Frame = 0

Query: 1   MSTARPKTPAAQLAISLSQQVDKEERSLRHLSDDVIMNQMYTKHNDDDTIKIDVNNYILF 60
           MS  RPK+ AA LA++LSQQ DKEE  L+HLSDDVI N+++    D++  KID++NY+LF
Sbjct: 1   MSLLRPKSSAAALALALSQQTDKEEPGLKHLSDDVITNRIFDFMTDEEMTKIDLDNYVLF 60

Query: 61  IESVIKTADEIALALHGAQGSKGHFVFSDDSSKFPSSIDPPICTLHEISRELACKAPGIE 120
           IE+V+K +D+I +A H AQGSKGHFV ++D+  +PS IDPPICTLH+IS E+ACK PG+E
Sbjct: 61  IENVLKASDQICVASHWAQGSKGHFVLAEDTMNYPSPIDPPICTLHQISSEMACKPPGVE 120

Query: 121 TAHKTTLDILSKLTRYPWDAKAVLIFAAFATNYGVMWHLDNYSHTDDPLAKSLAMIKRVA 180
           T HKTTLDILSKLTRYPW+AKAVLIF AF TNYGV+WHLDN+SH+ DPLAKSLA IKRV 
Sbjct: 121 TVHKTTLDILSKLTRYPWEAKAVLIFTAFVTNYGVLWHLDNHSHS-DPLAKSLATIKRVG 180

Query: 181 LLRKELDSLKYGQVFFDSNSLIYSCLKAIKYMNEIKNSSKYDTKEVPELSAALRQIPLVS 240
           LLRKELDS+KYGQVFF+ NS+IY+CLKA+KY+NE K+ SKYDTK+VPELSAALRQIPLVS
Sbjct: 181 LLRKELDSVKYGQVFFNQNSMIYNCLKAVKYINEFKSLSKYDTKDVPELSAALRQIPLVS 240

Query: 241 YWIIHTLVASSTELHCYLSGAEGQTQKYLNELTEKFQTLLYTLEKHLQFIREQQGEVELY 300
           YWIIH LVASS ELHCYLSG EGQTQKYLNE+TEK  +L+ TLE H+QFIREQQ EVELY
Sbjct: 241 YWIIHILVASSIELHCYLSGVEGQTQKYLNEMTEKVSSLVLTLENHMQFIREQQEEVELY 300

Query: 301 RWLVDQTDHFPTDITLFLSKLIDGRHKARPLINCSTQMEEYIEDFLKDKNLILVVSKSVE 360
           RWLVDQTDHFPTD+TLFL+KLI+G+HKARPLINCSTQ+EE+IE+F+K+K LIL+VS+S+ 
Sbjct: 301 RWLVDQTDHFPTDVTLFLAKLINGKHKARPLINCSTQLEEHIEEFMKEKKLILIVSRSLN 360

Query: 361 ISKEDLESLNLIYSELKKGNNYEIVWIPVISDPPNDGDEEAYEYLKSKMKWYAMPFTTKI 420
            S+EDLE L  +Y E+KK N +E+VWIPVISDPPNDGDEEAYE LKS+MKW+ +PF TKI
Sbjct: 361 FSREDLEILQSVYKEVKKENKFEMVWIPVISDPPNDGDEEAYEALKSEMKWFVVPFDTKI 420

Query: 421 AGVRFLEEKWELREDLLVVVLNTQSKIEFTNAIHLIRVWEKEAIPLTYERAKALLKKNWI 480
           AGVRFLEE+WELREDLL+VVL+TQSK+EF+NAIHL RVWEKEAIP +Y+R KAL+KKNWI
Sbjct: 421 AGVRFLEERWELREDLLLVVLDTQSKVEFSNAIHLTRVWEKEAIPFSYDRVKALMKKNWI 480

Query: 481 DSTVVKFSDEPRLNSLVMINQERNIIFYGGHNPRWIKNFEETADAIKRDPLTREEGISFE 540
           DSTV+KF+D+PRL SLV+INQERN+IFYGGHNPRW+KNFE++A+A+KRDP+TREEGI+FE
Sbjct: 481 DSTVLKFTDQPRLKSLVVINQERNVIFYGGHNPRWVKNFEDSAEAMKRDPVTREEGITFE 540

Query: 541 ILPVGLNNKGEQDPAVMFRFWMAQRSFFILKHQLQGSTASEDISRLISYENENGWAIIAK 600
           ++PVG NNKGE DP VMF FWMAQRS+FILKHQL GSTASEDISRLISYE E GWAII K
Sbjct: 541 LVPVGRNNKGEHDPTVMFNFWMAQRSYFILKHQLHGSTASEDISRLISYETEKGWAIITK 600

Query: 601 GPSVVFVGGGDLIIKAMEEFKLWKKNIRRVGFSGSFKDYFDELTATSLHCTHVNIIGFSG 660
           GP+VVF+GGGDLI+KA++EF++WKKN+RRVGFSGS+KDYFDELTA+SLHCTHVNIIGFSG
Sbjct: 601 GPTVVFIGGGDLILKAIDEFQIWKKNLRRVGFSGSYKDYFDELTASSLHCTHVNIIGFSG 660

Query: 661 WIPLIITCPVCRRYMGSGIRFTCCHGGPDVL 692
           WIPLIITCPVCRRYMGSGIRFTCCHGGPDVL
Sbjct: 661 WIPLIITCPVCRRYMGSGIRFTCCHGGPDVL 690

BLAST of Lcy01g002960 vs. NCBI nr
Match: XP_022958758.1 (protein SIEVE ELEMENT OCCLUSION B-like [Cucurbita moschata])

HSP 1 Score: 1143.3 bits (2956), Expect = 0.0e+00
Identity = 533/691 (77.13%), Postives = 626/691 (90.59%), Query Frame = 0

Query: 1   MSTARPKTPAAQLAISLSQQVDKEERSLRHLSDDVIMNQMYTKHNDDDTIKIDVNNYILF 60
           MS  RPK+ AA LA++LSQQ DKEE  L+HLSDDVI N+++    D++  KID++NY+LF
Sbjct: 1   MSLLRPKSSAAALALALSQQTDKEEPGLKHLSDDVITNRIFDFTTDEEMTKIDLDNYVLF 60

Query: 61  IESVIKTADEIALALHGAQGSKGHFVFSDDSSKFPSSIDPPICTLHEISRELACKAPGIE 120
           IE+V+K +D+I +A H AQGSKGHFV ++D+  +PS IDPPICTLH+IS E+ACK PG+E
Sbjct: 61  IENVLKASDQICVASHWAQGSKGHFVLAEDTMNYPSPIDPPICTLHQISSEMACKPPGVE 120

Query: 121 TAHKTTLDILSKLTRYPWDAKAVLIFAAFATNYGVMWHLDNYSHTDDPLAKSLAMIKRVA 180
           T HKTTLDILSKLTRYPW+AKAVLIF AF TNYGV+WHLDN+SH+ DPLAKSLAMIKRV 
Sbjct: 121 TVHKTTLDILSKLTRYPWEAKAVLIFTAFVTNYGVLWHLDNHSHS-DPLAKSLAMIKRVG 180

Query: 181 LLRKELDSLKYGQVFFDSNSLIYSCLKAIKYMNEIKNSSKYDTKEVPELSAALRQIPLVS 240
           LLRKEL+S+KYGQVFF+ NS+IY+CLKA+KY+NE K+ SKYDTK+VPELSAALRQIPLVS
Sbjct: 181 LLRKELESVKYGQVFFNQNSMIYNCLKAVKYINEFKSLSKYDTKDVPELSAALRQIPLVS 240

Query: 241 YWIIHTLVASSTELHCYLSGAEGQTQKYLNELTEKFQTLLYTLEKHLQFIREQQGEVELY 300
           YWIIH LVASS ELHCYLSG EGQTQKYLNE+TEK  +L+ TLE H+QFIREQQ EVELY
Sbjct: 241 YWIIHILVASSIELHCYLSGVEGQTQKYLNEMTEKVSSLVLTLENHMQFIREQQEEVELY 300

Query: 301 RWLVDQTDHFPTDITLFLSKLIDGRHKARPLINCSTQMEEYIEDFLKDKNLILVVSKSVE 360
           RWLVDQTDHFPTD+TLFL+KLI+G+HKARPLINCSTQ+EE+IE+F+K+K LIL+VS+S+ 
Sbjct: 301 RWLVDQTDHFPTDVTLFLAKLINGKHKARPLINCSTQLEEHIEEFMKEKKLILIVSRSLN 360

Query: 361 ISKEDLESLNLIYSELKKGNNYEIVWIPVISDPPNDGDEEAYEYLKSKMKWYAMPFTTKI 420
            S+EDLE L  +Y E+KK N +E+VWIPVISDPPNDGDEEAYE LKS+MKW+ +PF  KI
Sbjct: 361 FSREDLEILQSVYKEVKKENKFEMVWIPVISDPPNDGDEEAYEALKSEMKWFVVPFAAKI 420

Query: 421 AGVRFLEEKWELREDLLVVVLNTQSKIEFTNAIHLIRVWEKEAIPLTYERAKALLKKNWI 480
           AGVRFLEE+WELREDLL+VVL+TQSK+EF+NAIHL RVWEKEAIP +Y+R KAL+KKNWI
Sbjct: 421 AGVRFLEERWELREDLLLVVLDTQSKVEFSNAIHLTRVWEKEAIPFSYDRVKALMKKNWI 480

Query: 481 DSTVVKFSDEPRLNSLVMINQERNIIFYGGHNPRWIKNFEETADAIKRDPLTREEGISFE 540
           DSTV+K++D+PRL SLV+INQERN+IFYGGHNPRW+KNFE++A+A+KRDP+TREEGI+FE
Sbjct: 481 DSTVLKYTDQPRLKSLVVINQERNVIFYGGHNPRWVKNFEDSAEAMKRDPVTREEGITFE 540

Query: 541 ILPVGLNNKGEQDPAVMFRFWMAQRSFFILKHQLQGSTASEDISRLISYENENGWAIIAK 600
           ++PVG NNKGE DPAVMF FWMAQRS+FILKHQL GSTASEDISRLISYE E GWAII K
Sbjct: 541 LVPVGRNNKGEHDPAVMFNFWMAQRSYFILKHQLHGSTASEDISRLISYETEKGWAIITK 600

Query: 601 GPSVVFVGGGDLIIKAMEEFKLWKKNIRRVGFSGSFKDYFDELTATSLHCTHVNIIGFSG 660
           GP VVF+GGGDLI+KA++EF++WKKN+RRVGFSGSFKDYFDELTA+SLHCTHVNIIGFSG
Sbjct: 601 GPIVVFIGGGDLILKAIDEFQVWKKNLRRVGFSGSFKDYFDELTASSLHCTHVNIIGFSG 660

Query: 661 WIPLIITCPVCRRYMGSGIRFTCCHGGPDVL 692
           WIPLIITCPVCRRYMGSGIRFTCCHGGPDVL
Sbjct: 661 WIPLIITCPVCRRYMGSGIRFTCCHGGPDVL 690

BLAST of Lcy01g002960 vs. NCBI nr
Match: KAG7013505.1 (Protein SIEVE ELEMENT OCCLUSION B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1140.6 bits (2949), Expect = 0.0e+00
Identity = 531/691 (76.85%), Postives = 625/691 (90.45%), Query Frame = 0

Query: 1   MSTARPKTPAAQLAISLSQQVDKEERSLRHLSDDVIMNQMYTKHNDDDTIKIDVNNYILF 60
           MS  RPK+ A  LA++LSQQ DKEE  L+HLSDDVI N+++    D++  KID++NY+LF
Sbjct: 1   MSLLRPKSSATALALALSQQTDKEEPGLKHLSDDVITNRIFDFMTDEEMTKIDLDNYVLF 60

Query: 61  IESVIKTADEIALALHGAQGSKGHFVFSDDSSKFPSSIDPPICTLHEISRELACKAPGIE 120
           IE+V+K +D+I +A H AQGSKGHFV ++D+  +PS IDPPICTLH+IS E+ACK PG+E
Sbjct: 61  IENVLKASDQICVASHWAQGSKGHFVLAEDTMNYPSPIDPPICTLHQISSEMACKPPGVE 120

Query: 121 TAHKTTLDILSKLTRYPWDAKAVLIFAAFATNYGVMWHLDNYSHTDDPLAKSLAMIKRVA 180
           T HKTTL+ILSKLTRYPW+AKAVLIF AF TNYGV+WHLDN+SH+ DPLAKSLA IKRVA
Sbjct: 121 TVHKTTLNILSKLTRYPWEAKAVLIFTAFVTNYGVLWHLDNHSHS-DPLAKSLATIKRVA 180

Query: 181 LLRKELDSLKYGQVFFDSNSLIYSCLKAIKYMNEIKNSSKYDTKEVPELSAALRQIPLVS 240
           LLRKELDS+KYGQVFF+ NS+IY+CLKA+KY+NE K+ SKYDTK+VPELSAALRQIPLVS
Sbjct: 181 LLRKELDSVKYGQVFFNQNSMIYNCLKAVKYINEFKSLSKYDTKDVPELSAALRQIPLVS 240

Query: 241 YWIIHTLVASSTELHCYLSGAEGQTQKYLNELTEKFQTLLYTLEKHLQFIREQQGEVELY 300
           YWIIH LVASS ELHCYLSG EGQTQKYLNE+TEK  +L+ TLE H+QFIREQQ EVELY
Sbjct: 241 YWIIHILVASSIELHCYLSGVEGQTQKYLNEMTEKVSSLVLTLENHMQFIREQQEEVELY 300

Query: 301 RWLVDQTDHFPTDITLFLSKLIDGRHKARPLINCSTQMEEYIEDFLKDKNLILVVSKSVE 360
           RWLVDQTDHFPTD+TLFL+KLI+G+HKARPLINCSTQ+EE+IE+F+K+K LIL+VS+S+ 
Sbjct: 301 RWLVDQTDHFPTDVTLFLAKLINGKHKARPLINCSTQLEEHIEEFMKEKKLILIVSRSLN 360

Query: 361 ISKEDLESLNLIYSELKKGNNYEIVWIPVISDPPNDGDEEAYEYLKSKMKWYAMPFTTKI 420
            S+EDLE L  +Y E+KK N +E+VWIPVISDPPNDGDEEAYE LKS+MKW+ +PF  KI
Sbjct: 361 FSREDLEILQSVYKEVKKENKFEMVWIPVISDPPNDGDEEAYEALKSEMKWFVVPFAAKI 420

Query: 421 AGVRFLEEKWELREDLLVVVLNTQSKIEFTNAIHLIRVWEKEAIPLTYERAKALLKKNWI 480
           AGVRFLEE+WELREDLL+VVL+TQSK+EF+NAIHL RVWEKEAIP +Y+R KAL+KKNWI
Sbjct: 421 AGVRFLEERWELREDLLLVVLDTQSKVEFSNAIHLTRVWEKEAIPFSYDRVKALMKKNWI 480

Query: 481 DSTVVKFSDEPRLNSLVMINQERNIIFYGGHNPRWIKNFEETADAIKRDPLTREEGISFE 540
           DSTV+K++D+PRL SLV+INQERN+IFYGGHNPRW+KNFE++A+A+KRDP+TREEGI+FE
Sbjct: 481 DSTVLKYTDQPRLKSLVVINQERNVIFYGGHNPRWVKNFEDSAEAMKRDPVTREEGITFE 540

Query: 541 ILPVGLNNKGEQDPAVMFRFWMAQRSFFILKHQLQGSTASEDISRLISYENENGWAIIAK 600
           ++PVG NNKGE DPAVMF FWMAQRS+FILKHQL GSTASEDISRLISYE E GWAII K
Sbjct: 541 LVPVGRNNKGEHDPAVMFNFWMAQRSYFILKHQLHGSTASEDISRLISYETEKGWAIITK 600

Query: 601 GPSVVFVGGGDLIIKAMEEFKLWKKNIRRVGFSGSFKDYFDELTATSLHCTHVNIIGFSG 660
           GP+VVF+GGGDLI+KA++EF++WKKN+RRVGFSGSFKDYFDELTA+ LHCTHVNIIGFSG
Sbjct: 601 GPTVVFIGGGDLILKAIDEFQIWKKNLRRVGFSGSFKDYFDELTASGLHCTHVNIIGFSG 660

Query: 661 WIPLIITCPVCRRYMGSGIRFTCCHGGPDVL 692
           WIPLIITCPVCRRYMGSGIRFTCCHGGPDVL
Sbjct: 661 WIPLIITCPVCRRYMGSGIRFTCCHGGPDVL 690

BLAST of Lcy01g002960 vs. NCBI nr
Match: KAG6574937.1 (Protein SIEVE ELEMENT OCCLUSION B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1140.2 bits (2948), Expect = 0.0e+00
Identity = 531/691 (76.85%), Postives = 625/691 (90.45%), Query Frame = 0

Query: 1   MSTARPKTPAAQLAISLSQQVDKEERSLRHLSDDVIMNQMYTKHNDDDTIKIDVNNYILF 60
           MS  RPK+ A  LA++LSQQ DKEE  L+HLSDDVI N+++    D++  KID++NY+LF
Sbjct: 1   MSLLRPKSSATALALALSQQTDKEEPGLKHLSDDVITNRIFDFMTDEEMTKIDLDNYVLF 60

Query: 61  IESVIKTADEIALALHGAQGSKGHFVFSDDSSKFPSSIDPPICTLHEISRELACKAPGIE 120
           IE+V+K +D+I +A H AQGSKGHFV ++D+  +PS IDPPICTLH+IS E+ACK PG+E
Sbjct: 61  IENVLKASDQICVASHWAQGSKGHFVLAEDTMNYPSPIDPPICTLHQISSEMACKPPGVE 120

Query: 121 TAHKTTLDILSKLTRYPWDAKAVLIFAAFATNYGVMWHLDNYSHTDDPLAKSLAMIKRVA 180
           T HKTTLDILSKLTRYPW+AKAVLIF AF TNYGV+WHLDN+SH+ DPLAKSLA IKRVA
Sbjct: 121 TVHKTTLDILSKLTRYPWEAKAVLIFTAFVTNYGVLWHLDNHSHS-DPLAKSLATIKRVA 180

Query: 181 LLRKELDSLKYGQVFFDSNSLIYSCLKAIKYMNEIKNSSKYDTKEVPELSAALRQIPLVS 240
           LLRKELDS+KYGQVFF+ NS+IY+CLKA+KY+NE K+ SKYDTK+VPELSAALRQIPLVS
Sbjct: 181 LLRKELDSVKYGQVFFNQNSMIYNCLKAVKYINEFKSLSKYDTKDVPELSAALRQIPLVS 240

Query: 241 YWIIHTLVASSTELHCYLSGAEGQTQKYLNELTEKFQTLLYTLEKHLQFIREQQGEVELY 300
           YWIIH LVASS ELHCYLSG EGQTQKYLNE+TEK  +L+ TLE H+QFIREQQ EVELY
Sbjct: 241 YWIIHILVASSIELHCYLSGVEGQTQKYLNEMTEKVSSLVLTLENHMQFIREQQEEVELY 300

Query: 301 RWLVDQTDHFPTDITLFLSKLIDGRHKARPLINCSTQMEEYIEDFLKDKNLILVVSKSVE 360
           RWLVDQTDHFPTD+TLFL+KLI+G+HKARPLINCSTQ+EE+IE+F+K+K LIL+VS+S+ 
Sbjct: 301 RWLVDQTDHFPTDVTLFLAKLINGKHKARPLINCSTQLEEHIEEFMKEKKLILIVSRSLN 360

Query: 361 ISKEDLESLNLIYSELKKGNNYEIVWIPVISDPPNDGDEEAYEYLKSKMKWYAMPFTTKI 420
            S+EDLE L  +Y E+KK N +E+VWIPVISDPPNDGDEEAYE LKS+MKW+ +PF  KI
Sbjct: 361 FSREDLEILQSVYKEVKKENKFEMVWIPVISDPPNDGDEEAYEALKSEMKWFVVPFAAKI 420

Query: 421 AGVRFLEEKWELREDLLVVVLNTQSKIEFTNAIHLIRVWEKEAIPLTYERAKALLKKNWI 480
           AGVRFLEE+WELREDLL+VVL+TQSK+EF+NAIHL RVWEKEAIP +Y+R KAL+KKNWI
Sbjct: 421 AGVRFLEERWELREDLLLVVLDTQSKVEFSNAIHLTRVWEKEAIPFSYDRVKALMKKNWI 480

Query: 481 DSTVVKFSDEPRLNSLVMINQERNIIFYGGHNPRWIKNFEETADAIKRDPLTREEGISFE 540
           DSTV+K++D+PRL SLV+INQERN+IFYGGHNPRW+KNFE++A+A+KRDP+TREEGI+FE
Sbjct: 481 DSTVLKYTDQPRLKSLVVINQERNVIFYGGHNPRWVKNFEDSAEAMKRDPVTREEGITFE 540

Query: 541 ILPVGLNNKGEQDPAVMFRFWMAQRSFFILKHQLQGSTASEDISRLISYENENGWAIIAK 600
           ++PVG NNKGE DPAVMF FWMAQRS+FILKHQL GSTASEDIS+LISYE E GWAII K
Sbjct: 541 LVPVGRNNKGEHDPAVMFNFWMAQRSYFILKHQLHGSTASEDISQLISYETEKGWAIITK 600

Query: 601 GPSVVFVGGGDLIIKAMEEFKLWKKNIRRVGFSGSFKDYFDELTATSLHCTHVNIIGFSG 660
           GP+VVF+GGGD I+KA++EF++WKKN+RRVGFSGSFKDYFDELTA+SLHCTHVNIIGFSG
Sbjct: 601 GPTVVFIGGGDSILKAIDEFQIWKKNLRRVGFSGSFKDYFDELTASSLHCTHVNIIGFSG 660

Query: 661 WIPLIITCPVCRRYMGSGIRFTCCHGGPDVL 692
           WIPLIITCPVCRRYMGSGIRFTCCHGGPDVL
Sbjct: 661 WIPLIITCPVCRRYMGSGIRFTCCHGGPDVL 690

BLAST of Lcy01g002960 vs. NCBI nr
Match: XP_023006702.1 (protein SIEVE ELEMENT OCCLUSION B-like [Cucurbita maxima])

HSP 1 Score: 1133.6 bits (2931), Expect = 0.0e+00
Identity = 531/692 (76.73%), Postives = 628/692 (90.75%), Query Frame = 0

Query: 1   MSTARPKTPAAQLAISLSQQVDKEERSLRHLSDDVIMNQMYTKHNDDDTIKIDVNNYILF 60
           MS  R K+ A  LA++LSQQ DKEE  L+HLSDDVI N+++    D++ +KID++NY++F
Sbjct: 1   MSLLRSKSSATALALALSQQTDKEEPDLKHLSDDVITNRIFNFTTDEEMMKIDLDNYVVF 60

Query: 61  IESVIKTADEIALALHGAQGSKGHFVFSDDSSKFPSS-IDPPICTLHEISRELACKAPGI 120
           IE+V+K +D+I +A H AQGSKG+FV ++D+  +PSS IDPPICTLH+IS E+ACK PG+
Sbjct: 61  IENVLKASDQICVASHWAQGSKGNFVLAEDTMNYPSSPIDPPICTLHQISSEMACKPPGV 120

Query: 121 ETAHKTTLDILSKLTRYPWDAKAVLIFAAFATNYGVMWHLDNYSHTDDPLAKSLAMIKRV 180
           ET HKTTLDILSKLTRYPW+AKA+LIFAAF TNYGV+WHLDN+SH+ DPLAKSLAMIKRV
Sbjct: 121 ETVHKTTLDILSKLTRYPWEAKAMLIFAAFMTNYGVLWHLDNHSHS-DPLAKSLAMIKRV 180

Query: 181 ALLRKELDSLKYGQVFFDSNSLIYSCLKAIKYMNEIKNSSKYDTKEVPELSAALRQIPLV 240
            LLRKEL+S+KYGQVFF+ NS+IY+CLKA+KY+ E K+ SKYDTK+VPELSAALR+IPLV
Sbjct: 181 GLLRKELESVKYGQVFFNQNSMIYNCLKAVKYIIEFKSLSKYDTKDVPELSAALRRIPLV 240

Query: 241 SYWIIHTLVASSTELHCYLSGAEGQTQKYLNELTEKFQTLLYTLEKHLQFIREQQGEVEL 300
           SYWIIH LVASS ELHCYLSG EGQTQKYLNE+TEK  TL+ TLE H+QFIREQQ EVEL
Sbjct: 241 SYWIIHILVASSIELHCYLSGVEGQTQKYLNEMTEKVSTLVLTLENHMQFIREQQEEVEL 300

Query: 301 YRWLVDQTDHFPTDITLFLSKLIDGRHKARPLINCSTQMEEYIEDFLKDKNLILVVSKSV 360
           YRWLVDQTDHFPTD+TLFL+KLI+G+HKARPLINCSTQ+EE+IE+F+K+K LIL+VS+S+
Sbjct: 301 YRWLVDQTDHFPTDVTLFLAKLINGKHKARPLINCSTQLEEHIEEFMKEKKLILIVSRSL 360

Query: 361 EISKEDLESLNLIYSELKKGNNYEIVWIPVISDPPNDGDEEAYEYLKSKMKWYAMPFTTK 420
             S+EDLE L  IY E+KK N +E+VWIPVISDPPNDGDEEAYE LKS+MKW+ +PF TK
Sbjct: 361 NFSREDLEILQSIYKEVKKENKFEMVWIPVISDPPNDGDEEAYEALKSEMKWFVVPFATK 420

Query: 421 IAGVRFLEEKWELREDLLVVVLNTQSKIEFTNAIHLIRVWEKEAIPLTYERAKALLKKNW 480
           IAGVRFLEE+WELREDLL+VVL+TQSK+EF+NAIHL RVWEKEAIPL+Y+R K L+KKNW
Sbjct: 421 IAGVRFLEERWELREDLLLVVLDTQSKVEFSNAIHLTRVWEKEAIPLSYDRVKVLMKKNW 480

Query: 481 IDSTVVKFSDEPRLNSLVMINQERNIIFYGGHNPRWIKNFEETADAIKRDPLTREEGISF 540
           IDSTV+KF+D+PRL SLV+INQERN+IFYGGHNPRW+KNFEE+A+A+KRDP+TREEGI+F
Sbjct: 481 IDSTVLKFTDQPRLKSLVVINQERNVIFYGGHNPRWVKNFEESAEAMKRDPVTREEGITF 540

Query: 541 EILPVGLNNKGEQDPAVMFRFWMAQRSFFILKHQLQGSTASEDISRLISYENENGWAIIA 600
           E++PVG NNKGE DPAVMF FWMAQRS+FILKHQL GSTASEDISR+ISYE E GWAII 
Sbjct: 541 ELVPVGRNNKGEHDPAVMFNFWMAQRSYFILKHQLHGSTASEDISRMISYETEKGWAIIT 600

Query: 601 KGPSVVFVGGGDLIIKAMEEFKLWKKNIRRVGFSGSFKDYFDELTATSLHCTHVNIIGFS 660
           KGP+VVF+GGGDLI+KA++EF++WKKN+R+VGFSGSFKDYFDELTA+SLHCTHVNIIGFS
Sbjct: 601 KGPTVVFIGGGDLILKAIDEFQIWKKNLRQVGFSGSFKDYFDELTASSLHCTHVNIIGFS 660

Query: 661 GWIPLIITCPVCRRYMGSGIRFTCCHGGPDVL 692
           GWIPLIITCPVCRRYMGSGIRFTCCHGGPDVL
Sbjct: 661 GWIPLIITCPVCRRYMGSGIRFTCCHGGPDVL 691

BLAST of Lcy01g002960 vs. TAIR 10
Match: AT3G01680.1 (CONTAINS InterPro DOMAIN/s: Mediator complex subunit Med28 (InterPro:IPR021640); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G01670.1); Has 122 Blast hits to 112 proteins in 13 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 122; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 216.9 bits (551), Expect = 5.3e-56
Identity = 185/712 (25.98%), Postives = 317/712 (44.52%), Query Frame = 0

Query: 32  SDDVIMNQMYTKHNDDDTIKIDVNNYILFIESVIKTADEIALALHGAQGSKGHFVFSDD- 91
           SD+ +M ++  + +  D  ++ V   +  +E ++   D   L       S       D  
Sbjct: 38  SDESMMLKLIQQTHSPDAREVQVRGLLSLVEDIL---DRATLDSEDTNASMLPLPTEDKL 97

Query: 92  -SSKFPSSIDPPICTLHEISRELACKAPGIETAHKTTLDILSKLTRYPWDAKAVLIFAAF 151
             S   S +D     +  ++ E+A K+     +H+ T+ +   L+ + WD K VL  AAF
Sbjct: 98  MQSSMMSVLDSVSYAIDRVACEIAYKSLTGSDSHEITMSVFEHLSSFQWDGKLVLTLAAF 157

Query: 152 ATNYGVMWHLDNYSHTDDPLAKSLAMIKRVALL-RKELDSLKYGQVFFDSNSLIYSCLKA 211
           A NYG  W L  + ++ + LAKSLAM+K V +  R  L+S+  G      N LI      
Sbjct: 158 ALNYGEFWLLVQF-YSKNQLAKSLAMLKLVPVQNRVTLESVSQG-----LNDLIREMKSV 217

Query: 212 IKYMNEIKN-SSKYDTKEVPELSAALRQIPLVSYWIIHTLVASSTELHCYLS-GAE---- 271
              + E+     +Y T +VP+LS  L  IP+  YW I +++A  ++++   + G E    
Sbjct: 218 TACVVELSELPDRYITPDVPQLSRILSTIPIAVYWTIRSVIACISQINMITAMGHEMMNT 277

Query: 272 ----GQTQKYLNELTEKFQTLLYTLEKHLQFIREQQGEVELYRWLVDQTDHFPTDITLFL 331
                +T    N+L      L  TL    + I E+Q   E  + L    D    D    L
Sbjct: 278 QMDLWETSMLANKLKNIHDHLAETLRLCYRHI-EKQRSSESLKVLHSLFDTTHIDNMKIL 337

Query: 332 SKLIDGRHKARPLINCSTQMEEYIEDFLKDKNLILVVSKSVEISKEDLESLNLIYSELKK 391
           + L+  +    PL +  T+ + ++ D L+ K ++L++S  + I +++L     IY+E ++
Sbjct: 338 TALVHPKPHITPLQDGLTKRKVHL-DVLRRKTVLLLIS-DLNILQDELSIFEQIYTESRR 397

Query: 392 G---------NNYEIVWIPVISDPPNDGD-----EEAYEYLKSKMKWYAM--PFTTKIAG 451
                       YE+VW+PV+ DP  D +     ++ +E L+  M WY++  P   +   
Sbjct: 398 NLVGVDGKSHMPYEVVWVPVV-DPIEDFERSPILQKKFEDLRDPMPWYSVDSPKLIERHV 457

Query: 452 VRFLEEKWELREDLLVVVLNTQSKIEFTNAIHLIRVWEKEAIPLTYERAKALLKKNWID- 511
           V F+  +W      ++VV++ Q      NA+H+I +W  EA P T  R + L ++     
Sbjct: 458 VEFMRGRWHFMNKPILVVIDPQGNEASLNALHMIWIWGTEAFPFTRSREEELWRRETFSL 517

Query: 512 STVVKFSDEPRLNSLVMINQERNIIFYGGHNPRWIKNFEETADAIKRDPLTREEGISFEI 571
           + +V   D    N    I  +  I  YGG +  WI+ F   A A  +D       ++ E+
Sbjct: 518 NLIVDGIDSVIFN---WIKPDNYIFLYGGDDLDWIRRFTMAAKATAKD-----SNVNLEM 577

Query: 572 LPVGLNNKGEQ--------------------DPAVMFRFWMAQRSFFILKHQLQGSTASE 631
             VG  N   +                    +PA+M+ FW    S    K QL  +   +
Sbjct: 578 AYVGKRNHSHREQIRRISEVIRSENLSHSWAEPALMWFFWTRLESMLYSKIQLGKADDHD 637

Query: 632 D----ISRLISYENENGWAIIAKGPSVVFVGGGDLIIKAMEEFKLWKKNIRRVGFSGSFK 686
           D    I +++SY+   GWA+++KGP +V +  G +        + WK ++   G++ +  
Sbjct: 638 DVMQGIKKILSYDKLGGWALLSKGPEIVMIAHGAIERTMSVYDRTWKTHVPTKGYTKAMS 697

BLAST of Lcy01g002960 vs. TAIR 10
Match: AT3G01670.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G01680.1); Has 121 Blast hits to 111 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 121; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 174.5 bits (441), Expect = 3.0e-43
Identity = 184/758 (24.27%), Postives = 320/758 (42.22%), Query Frame = 0

Query: 6   PKTPAAQLAISLSQQVDKEERSLRHLSDDVIMNQMYTKHNDDDTIKIDVNNYILFIESVI 65
           P +   +      Q   +  R +  LSDD +M     K +  D I  DV + +  +  + 
Sbjct: 111 PNSLGGRFGPGKKQAFHRNGRPMFSLSDDRVMADRVLKTHSPDMIFFDVTSLLSVVNDIF 170

Query: 66  KTADEIALALHGAQGSKGHFVFSD--DSSKFPSSIDPPICTLHEISRELACK-------- 125
           K+     +    +   K   VF D  D + F +  D     + +IS E+ CK        
Sbjct: 171 KS----HVPSIDSSAPKPSLVFKDYADHTSFETFAD----LIDQISCEIDCKCLHGGESH 230

Query: 126 -----APGIETAHKTTLDILSKLTRYPWDAKAVLIFAAFATNYGVMWHLDNYSHTDDPLA 185
                   +++ + TT  +LS +++Y WDAK VL+ +A A  YGV   L   +H  + L 
Sbjct: 231 GMMTSGLHLDSRNTTTFSVLSLVSKYRWDAKLVLVLSALAVKYGVFLLLAE-THATNQLT 290

Query: 186 KSLAMIKRVALLRKELDSLKYGQVFFDSNSLIYSCLKAIKYMNEIKN--SSKYDTKEVP- 245
           KSLA+IK++              +F   N+L     K    M ++ +  ++  D  ++P 
Sbjct: 291 KSLALIKQLP------------SIFSRQNALHQRLDKTRILMQDMVDLTTTIIDIYQLPP 350

Query: 246 -ELSAALR-QIPLVSYWIIHTLVASSTELHCYLSGAEGQTQ---------KYLNELTEKF 305
             ++AA    IP   YWI+  ++   +    ++SGA G  Q           ++E +E+ 
Sbjct: 351 NHITAAFTDHIPTAVYWIVRCVLICVS----HISGASGFKQDQIMSFMEVSEIHENSERL 410

Query: 306 QT----LLYTLEKHLQFIREQQGEVELYRWLVDQTDHFPTDITLFLSKLI---------D 365
           +     LL   +K    I E   E E    +   T     D+   L +L+          
Sbjct: 411 RKINAYLLEQFKKSKMTIEEGIIEEEYQELIQTFTTIIHVDVVPPLLRLLRPIDFLYHGA 470

Query: 366 GRHKARPLINCSTQMEEYIEDFLKDKNLILVVSKSVEISKEDLESLNLIYSELKKGNNYE 425
           G  K R  IN  TQ           K+++L++S    I KE L  L  +Y+E  +  ++E
Sbjct: 471 GVSKRRVGINVLTQ-----------KHVLLLISDLENIEKE-LYILESLYTEAWQ-QSFE 530

Query: 426 IVWIPVISDPPNDGDEEAYEYLKSKMKWYAM--PFTTKIAGVRFLEEKWELREDLLVVVL 485
           I+W+PV  D   + D+  +E L   M+WY +  P   + A +RF+ E W  +   ++V L
Sbjct: 531 ILWVPV-QDFWTEADDAKFEALHMNMRWYVLGEPRKLRRAAIRFVREWWGFKNRPILVAL 590

Query: 486 NTQSKIEFTNAIHLIRVWEKEAIPLTYERAKAL-LKKNWIDSTVVKFSDEPRLNSLVMIN 545
           + + ++  TNA  ++ +W+  A P T  R + L  ++ W    ++  +D   LN LV   
Sbjct: 591 DPKGQVMSTNAFPMVWIWQPFAHPFTTARERDLWSEQEWNLEFLIDGTDPHSLNQLV--- 650

Query: 546 QERNIIFYGGHNPRWIKNFEETADAIKRDPLTREEGISFEILPVGLNN-KGEQDPAV--- 605
             + I  YGG + +WIKNF     ++ R+ + +   I  E++ VG  N K    P +   
Sbjct: 651 DGKYICLYGGEDMQWIKNF----TSLWRN-VAKAANIQLEMVYVGKRNPKNGIQPIINTI 710

Query: 606 -----------MFRFWM---------AQRSFFILKHQLQG---------STASEDISRLI 665
                      +F+ W            +   +  H ++G             +++  ++
Sbjct: 711 REENLSHTLPDLFQIWFFWTRVESMWESKQRMLKAHGIKGREGFKEEEKDLVLQEVVAML 770

Query: 666 SYENE-NGWAIIAKGPSVVFVGGGDLIIKAMEEFKLWKKNIRRVGFSGSFKDYFDELTAT 685
            Y  E +GW +++K   ++    G+L  + + EF  W+ NI   GF  +  D+   +   
Sbjct: 771 GYGGEGDGWGLVSKASDMMVRAKGNLFSRGLAEFNEWEVNIPTKGFLTALNDHL-LMRLP 820

BLAST of Lcy01g002960 vs. TAIR 10
Match: AT1G67790.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G01680.1); Has 208 Blast hits to 125 proteins in 13 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 208; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 91.7 bits (226), Expect = 2.6e-18
Identity = 131/679 (19.29%), Postives = 262/679 (38.59%), Query Frame = 0

Query: 26  RSLRHLSDDVIMNQMYTKHNDDDTIKIDVNNYILFIESVIK--TADEIALALHGAQGSKG 85
           R +  L++D+I+ Q+   H D D   +D    +  +E+++     ++++  L        
Sbjct: 5   RDISALNEDIIVEQLLRSH-DPDGRWLDSEMLLQEVETILSFVLQNDVSRPLLTENCITT 64

Query: 86  HFVFSDDSSKFPSSIDPPICTLHEISRELACKAPGIETAHKTTLDILSKLTRYPWDAKAV 145
             VF D     P +I         IS ++ C   G     K T+ +   L  Y WDAKAV
Sbjct: 65  IEVF-DSKETLPYAI-------FRISVQMLCPCTGENEIRKRTMVLFDLLKEYRWDAKAV 124

Query: 146 LIFAAFATNYGVMWHLDNYSHTDDPLAKSLAMIKRVALLRKE----LDSLKYGQVFFDSN 205
           L+    A  YG +  L  +    DP+A S+A + ++ + R +    L+SL    +   + 
Sbjct: 125 LVLGVLAATYGGLL-LPVHLAICDPVAASIAKLNQLPIERTKFRPWLESL---NLLIKAM 184

Query: 206 SLIYSCLKAIKYMNEIKNSSKYDTKEVPELSAALRQIPLVSYWIIHTLVASSTELHCYLS 265
             +  C+  IK+       +K D   + E    L  I L +Y ++ + +    ++  +  
Sbjct: 185 VDVTKCI--IKFEKIPFKQAKLDNNILGE---TLSNIYLTTYRVVKSALTCMQQIPYF-- 244

Query: 266 GAEGQTQKY-LNELTEKFQTLLYTLEKHLQFIREQQGEVELYRWLVDQTDHFPTDITLFL 325
               QTQ+  + E+ +K   LL +           +  VE   +L+ Q    P+      
Sbjct: 245 ---KQTQQISITEVQDKVTLLLLS-----------KPPVEPLFFLLQQLYDHPS------ 304

Query: 326 SKLIDGRHKARPLINCSTQMEEYIEDFLKDKNLILVVSKSVEISKEDLESLNLIYSELKK 385
                         N +T+                                         
Sbjct: 305 --------------NTNTE----------------------------------------- 364

Query: 386 GNNYEIVWIPVISDPP-NDGDEEAYEYLKSKMKWYAM--PFTTKIAGVRFLEEKWELRE- 445
             NYEI+W+P+ S     D ++E +++  + + W ++  P+      + F +++W  ++ 
Sbjct: 365 -QNYEIIWVPIPSSQKWTDEEKEIFDFYSNSLPWISVRQPWLMSSTILNFFKQEWHYKDN 424

Query: 446 DLLVVVLNTQSKIEFTNAIHLIRVWEKEAIPLTYERAKALLKKNWIDSTVVKFSDEPRLN 505
           + ++VV+++  +    NA+ ++ +W  +A P +  R   L K++        +S    L+
Sbjct: 425 EAMLVVIDSNGRFVNMNAMDMVLIWGVKAYPFSVSREDELWKEH-------GWSINLLLD 484

Query: 506 SLVMINQERNIIFYGGHNPRWIKNFEETADAIKRDPLTREEGISFEILPVGLNNKGEQD- 565
            +    + R I  +G  N  WI  F   A  I        + + F++  + L+N+   + 
Sbjct: 485 GIHPTFEGREICIFGSENLDWIDEFVSLARKI--------QNLGFQLELIYLSNQRRDER 544

Query: 566 ----------PAVMFRFWMAQRSF------FILKHQLQGSTASEDISRLI--SYENENGW 625
                     P +   FW+   S        I+    +     E++  L+   Y    GW
Sbjct: 545 AMEESSILFSPTLQQLFWLRLESIERSKLKRIVIEPSKPDRVFEEVRNLLDFDYGKHRGW 566

Query: 626 AIIAKGPSVVFVGGGDLIIKAMEEFKLWKKNIRRVGFSGSFKDYFDELTATS-LHCTHVN 673
            II  G +   V  G+ + + M +   W +  + +GF+ +      E+ A      +H  
Sbjct: 605 GIIGNGSTAETV-DGEKMTERMRKIVRWGEYAKGLGFTEAI-----EIAAEKPCELSHTA 566