Lcy01g002640 (gene) Sponge gourd (P93075) v1

Overview
NameLcy01g002640
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionChlorophyllase
LocationChr01: 3095120 .. 3100815 (+)
RNA-Seq ExpressionLcy01g002640
SyntenyLcy01g002640
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGAAAGTGACACTGACAAACTCACAAACAGAAAAAGGGGTTGAAAATTGAAATCGGAACTTGAAGCTTCACTCGCCATCGCCATGGCTATGGCTTCTTTTTCTTTTCCTCCGCCGTCGGCCACCGCCGGCTCTGCAACTTCGCCCAATGTGTTCGAGATCGGAAAGTTCAACGCCGTGCTTGAAAAGGTCGATTCGGGATTTTGTTCGTCCGGCCGATATCTTCCGGTTCCGCCGCCAAAGCCGCTGCTGATCGGCCGGCCGTCCGACCCCGGAGAGTTCCCGGTGCTGCTGCTCATCCACGGCTATCTTCTTTACAACACTTTCTACTCTCAGCTCATTCAACAGATCGCTTCTCATGGCTTCATCGTCATCGCCCCTCAGGTTTCTCACTCTCTTCTTTTTCCATTTTTTTTATTTTTCGTATTCCTCATGTTCCCGGTGCTGCTGCTCATTTTTATTTATGGGTTAATCCGGTTAAAAACCACTTCTAGTCTTGAACTTAATTTCATGAAATTAACAATTTAGTTCCTGAATTTTGATTTATAACGATTTAGTCCATGTACTTTCAATTTTGTAGTAATTTAGGCCCCGTTTGATAACCATTTGGTTTTTAGTTTTTGGTTTTTGAAAATTATGCTTGTTTTCTCTAAATTTTCCTACATGGTTTTCATCTTTATCAAGGGCACATTTGAATTCTTAGCCAAATTCTAAAAACAAAAACAAGTTTTTGAAAACTACTTTTTTTAGTTTTCAAAATTTGGCTTGGTTTTTTAAAACATGGAAAATATGTATTAGAAAAACAAAGAAACTTGTAGGTGGAATTAGTGTTTACAAGCTTAATTTTCAAAAACCAAAAACCAAAAACCAAATGGTTATCAAACGAGGCTTAGTCTACATACTTTCAATTTTGTAACAAGTTAGTCTCTGAACTTTAATAAGTAACAATTTAATCATTATACTTTCAAATTTGTAACGATTTAGTCTATATCGTGAAAAATTAATGTAAAGATTTAATAAAATTTCTTAAATGTAAAGATTTAATAAAATTTCTTACTTAGATAGATTATTAAACTAATTTGGGACCCAATATGTTAATAAGCTATAAAGTTTAATCATTAAATAATAAAAATCGACATTTAATTTTGATGAATTTTTTAGCGACAAGGATTAAATAGTTACAAATTCGAATGTATAGGGACTAAATTGTTACAAATTTGAAACTATAGGAATTAAATTGTTACTTATTAAAGTTTAGAGATTAAATTTTTACAAAATTTAAAATACAGAGACTAAATCGTTACAAATTAATTTCAGGGACCAAATTATTACTTTCATGAAAGTTTAGGGATCGAAAGTGATTTTTAGCCTTATTCATTTATTTTGTTTTATTTTGTTTTGGTTATGGATGACTTGACTTGTTGTTCAAGGGAAAGAAAATATCTTTTTCTAAATATCTTTTTTTTTTTTTTTTTGGAGTATGACTTTAGAATTTATTAAGACAAAAACCATATATTTAAAAATATACCACTAAAATAAAGTATAAGATATAGGACTAAAATGATATTTTAACGAAATTTAAAAAATCACTTTAAAAACGTTTTCTAGGCACGCCCATTTTTCAAATGAACCGTTAAAATATTTTCATATTTAAAATTTGTTTCTTAGTACATTTTGATATTTTAATTGAGGTAAAGAAAGAAACAATTTTACCATCAATATCGACACCTAAAAATGAAAATAAATTACAGACATTTATTTAAATTATACATTATATTATAATGATTTTGGCAATAAAATTTCACAATTATGCTATAATGAGATGTTGTCATAAATTTAGTAATTATTATTTCAACTCAGCTTGGCAATGGAATTTGCTAGTGAACTTGAGGAGTCATCCCCAACTTCCTTTACAATGATGCCATAGTGAGATATTTTATCAAAATATCTTCTTTCCAGTCAACCTTTGGTTACAAACTCAATATGCACAAAAATGAAGGTGCTTTTTGAAAATAGTGTTAAAAGGCAATTCTAGACTTTTTGAAACAAAATTTTGCCTTTTCTTTTCCCCAACAATTGAGAATAAATTTTTACTTAAGTAGAAGAGTTAATAAAATTTAGGTTAAATTATCAAAAATACACATGTACTATGGTTGGTTTTAATTATATCCGTGAACTTTTAAAAGTTTCATTTTAGTTCTCAAAGTTTGAATTTTGTTTCAAAACAACCCTCAAAGTCATTTTTTCGTTAAATTTATGGACGAAAAATGACGTGTTTGCATATGTATTGTTGCTTGTGTGAACATATTTGAGCATATACGTGAGAAAAACAATAAAAACATATGCAATAAACACATCATTTTTCTTGTATATTAACATAAAATGTTTCCAATGGTCGTTTTGAAACAAACTTTAAACTTCAAGCGTTAAAATGAAACATTTGAAAGTGCGGGGTTGTAATTGAAACCAACCTCATGATTCAGGGATATTTTTGATAATTTAATCTAAAATTTACTGAACTATTAAATATGATACAATGTAGAAGTGAAATCACAAGGTTAGCCTCTGAACTTTGAAATTTGTGTTTATTTGATGCGACTTTAAAAATACCTAATAGTTCATATATAAACTTGTAATTTTGTGTCTAATATTAAAAAAATGTCTAATAAATTCTTAAACATTCAATTTTATGTCTAAGAGATGGTTTGCCTATTAAAAGATTTTAAAAATTCATTCATCTCTTACAAATATACCTAATATGTAAGTTAGTTTTAAAATATTCAAACATGTCAGAGGTCTATTATATTAAAAAATAAAAGATTGCAAGTTTGAGAGAATATTAGACTCTTCAAAGTTCAGTGACCTTAACCTATGTTATAAAATCTTAAAAGTTTAGGTCCCATTTGATAACCATTTGGTTTTTGGTTTTTTGTTTTTGAAAAACTATGTTTGTTTTCTCCTAGATTTCCTACCATGACTTTCATCTTAGTTAAAGTTGCATTTGAATTCCTAGCCAAATTTTAAGAACAAAAACAAGTTTTTGAAAATTTTTTTTTTTTTTTTTTTTTTTACAAAATTGGACTTGGTTTTTGAAAACAAGGTAGAAGATTGATACTAAAACCAAGAAACTTATAGCTGAAAATATGTATGTATAAGTTTAATTTGCAAAAACAAAAAACCAAATGGTTATTAAACGGAGTCTTAAAAAACCAAACTTGTGATTTAATCTACTATAAATTAATTAACAAAATAGATGCAAAGACTTTAAAAAAAAAGTTCTAGAAAATGTCATTCTAAAAAGCACACAAAATATCTCATATTTGAAGTTTGATAATAATTCATTTTAAGTGAAAATTATAGCTCCACGCCATAGCTTTGACACATAGACACTTGCAAGTTGCAAGTGCTGCGTTTTGTCAACAAAAATAAAGACAACCCCACTTCTTAAACCCATTATAATTTCTCACACAAAACAAAATAAAGGGTTTTTTTTTTCTTATTTAAAAAATGTTTCAAAATCTTTTTAGGAAAAAAAAAAACAATTTTTATCTTTGATCAAGTGGATGAACCTAAACTTTCAATTATCTCATCCAATTGTGTTACTTAAACTTTGATAAAATTTATAATATTTACCCTAATCTTGAACACTTTATAATTTTAACTTCAATTTTTTGTTTGAGTTAAAAAATTCATTACAAGATAATTCTAACCGCATCAAATGAATTATAAAATTTATATATGGTATGAAATTGATTTGACTTCCGAGAAAAATGATATTGGAATTGAATTTCTTTTTTCTTTTTTTTTTTTTGTTTGTAAAAATGGAGAGTTTTAAGAGATTTACTCAAAGATGGATGTTGTGCATCAACCAAAGCATAATTACTTATGGTTAATGCCTAATTACTCACCTTCCCATTAATCAGTAAGTATGGAAAGATAAAAGAGCTTTTTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTTAACATTTTTTAATGGTATATTCTTAATTGATAAATAGGTAAATATTATTCTTAACCAAATTTAGTGGAACACAAAAATAAAAAGGTTAAAAATCACTTTTAGTCCCTAAACTTTCATGAAAGTAACAATTTGGTCCCTAAACTTAGATTTGTAATGATTTAGTCCCTGTACTTTTAATTTTGTAACAATTTAGTCCCTGAACTTTAATAAGTAACAATTTAGTTTCTGTACTTTTAAACTTGCAACAATTTAGTCCATATACTTTCAAATTTGTAACAATTTAATCTTTGTTGCAAAAAAAAATCATCAAAATTAAATGTTGATTTTTATTATTTAATGATTAAACTTTGTAGCATATTAACATATCGGGTCCCAAATTAGTTTATTAATCTATCTAGGTAAGAAATTTCATTAAATCTTTACATTAATTTTTCACGATAGGGACTAAATTGTTACAAATTTGAAAGTACAGGAACTGAATTGTTACTTATTAAAGTTTAAGGGCTAAATAGTTACAAAATTAAAAGTATACAGACTAAATCGTTATAAAATTGAAAGTACAGGGACTAAATCGTTACAAATCAAAGTTTATGGACTAAATTATTACTTTCAAGAAAGTTTATGGACCAAAAGTGATGTTTAACCAAAAAAAAATCAGCTTTTTTTTTTTTTTTTTTTTTTTTTTGAAACTAAAAAAAAATCAGCTTATTTCATTTGTTTTTTTTTTTAAATGAAGTTCCTCTTAAAAAATCCATGTTCATCTCGAACTTGCTCTGTACAGTTGTACACATTGGCCGGACCCGATACAAGCGAAGAAATAAAAGCAACCGCCGCCATAATAAACTGGCTATCGGAAGGTCTCCGACAACTCCTTCCGCCGCACGTGAACCCAAATCTGAACAAAATAGCCCTCGCCGGCCACAGCCGCGGCGGCAAAACCTCTTTCGCCTTAGCCCAACACAAATCAATCCCGAAATCCCCAAAATTCTCAGCCCTAATCGGCCTCGATCCCGTGGACGGAATGGGCTCCGGCAAGCAAACCCATCCCCCGGTCCTCAAATACATCCCGCAATCGTTCGATTTCGAGCTCCCCGTTCTGGTAATCGGGTCGGGTCTGGGCGAATTCAAAAGAAACCCACTGTTCCCCCCTTGCGCGCCAAAGGGAATCAACCACGAGGACTTTTTCAACGAGTGTCGGAGTCCGGCGCACTACTTTGTGGTGAAGGATTACGGGCACTTGGATCTGTTGGACGATGAGACTGGAGGAATCAGAGGGAAGGCGAGTTATTGTCTGTGTAAGAATGGCGAATCCAGAGAGCCGATGCGGCGGTTTGTTGGTGGGGCTGTGGTGGCGTTTCTGAAGGCTTATTTGGATGGAGAAGATGAAGATTTGAGTGGCATTGTAGATGGGGATTTGAGCTTGCCTGTTCAACTTCAGACTGTTGAATCTCTTCTTTGAAATGGGGTTTGTGATTCGAATAAGAACCGAGTGAAACTTGAAGATGCAGATTTCCTATTTGCTTTATTTTTGGTTTACTTCATTTTTGTGTCTTTAATAATGGGTGTGAGAAAATTAAAGTTGATGTAGAATGGAAGAAGAAAGTTTATGGAAATTAGGATAGTTTAATTCCATTTAGGAAAAGACACAAGGTATATATTTGGAATTATCAAATTAGCATTTAGACAATGGTTGAGTGGATGATCG

mRNA sequence

GTGAAAGTGACACTGACAAACTCACAAACAGAAAAAGGGGTTGAAAATTGAAATCGGAACTTGAAGCTTCACTCGCCATCGCCATGGCTATGGCTTCTTTTTCTTTTCCTCCGCCGTCGGCCACCGCCGGCTCTGCAACTTCGCCCAATGTGTTCGAGATCGGAAAGTTCAACGCCGTGCTTGAAAAGGTCGATTCGGGATTTTGTTCGTCCGGCCGATATCTTCCGGTTCCGCCGCCAAAGCCGCTGCTGATCGGCCGGCCGTCCGACCCCGGAGAGTTCCCGGTGCTGCTGCTCATCCACGGCTATCTTCTTTACAACACTTTCTACTCTCAGCTCATTCAACAGATCGCTTCTCATGGCTTCATCGTCATCGCCCCTCAGTTGTACACATTGGCCGGACCCGATACAAGCGAAGAAATAAAAGCAACCGCCGCCATAATAAACTGGCTATCGGAAGGTCTCCGACAACTCCTTCCGCCGCACGTGAACCCAAATCTGAACAAAATAGCCCTCGCCGGCCACAGCCGCGGCGGCAAAACCTCTTTCGCCTTAGCCCAACACAAATCAATCCCGAAATCCCCAAAATTCTCAGCCCTAATCGGCCTCGATCCCGTGGACGGAATGGGCTCCGGCAAGCAAACCCATCCCCCGGTCCTCAAATACATCCCGCAATCGTTCGATTTCGAGCTCCCCGTTCTGGTAATCGGGTCGGGTCTGGGCGAATTCAAAAGAAACCCACTGTTCCCCCCTTGCGCGCCAAAGGGAATCAACCACGAGGACTTTTTCAACGAGTGTCGGAGTCCGGCGCACTACTTTGTGGTGAAGGATTACGGGCACTTGGATCTGTTGGACGATGAGACTGGAGGAATCAGAGGGAAGGCGAGTTATTGTCTGTGTAAGAATGGCGAATCCAGAGAGCCGATGCGGCGGTTTGTTGGTGGGGCTGTGGTGGCGTTTCTGAAGGCTTATTTGGATGGAGAAGATGAAGATTTGAGTGGCATTGTAGATGGGGATTTGAGCTTGCCTGTTCAACTTCAGACTGTTGAATCTCTTCTTTGAAATGGGGTTTGTGATTCGAATAAGAACCGAGTGAAACTTGAAGATGCAGATTTCCTATTTGCTTTATTTTTGGTTTACTTCATTTTTGTGTCTTTAATAATGGGTGTGAGAAAATTAAAGTTGATGTAGAATGGAAGAAGAAAGTTTATGGAAATTAGGATAGTTTAATTCCATTTAGGAAAAGACACAAGGTATATATTTGGAATTATCAAATTAGCATTTAGACAATGGTTGAGTGGATGATCG

Coding sequence (CDS)

ATGGCTATGGCTTCTTTTTCTTTTCCTCCGCCGTCGGCCACCGCCGGCTCTGCAACTTCGCCCAATGTGTTCGAGATCGGAAAGTTCAACGCCGTGCTTGAAAAGGTCGATTCGGGATTTTGTTCGTCCGGCCGATATCTTCCGGTTCCGCCGCCAAAGCCGCTGCTGATCGGCCGGCCGTCCGACCCCGGAGAGTTCCCGGTGCTGCTGCTCATCCACGGCTATCTTCTTTACAACACTTTCTACTCTCAGCTCATTCAACAGATCGCTTCTCATGGCTTCATCGTCATCGCCCCTCAGTTGTACACATTGGCCGGACCCGATACAAGCGAAGAAATAAAAGCAACCGCCGCCATAATAAACTGGCTATCGGAAGGTCTCCGACAACTCCTTCCGCCGCACGTGAACCCAAATCTGAACAAAATAGCCCTCGCCGGCCACAGCCGCGGCGGCAAAACCTCTTTCGCCTTAGCCCAACACAAATCAATCCCGAAATCCCCAAAATTCTCAGCCCTAATCGGCCTCGATCCCGTGGACGGAATGGGCTCCGGCAAGCAAACCCATCCCCCGGTCCTCAAATACATCCCGCAATCGTTCGATTTCGAGCTCCCCGTTCTGGTAATCGGGTCGGGTCTGGGCGAATTCAAAAGAAACCCACTGTTCCCCCCTTGCGCGCCAAAGGGAATCAACCACGAGGACTTTTTCAACGAGTGTCGGAGTCCGGCGCACTACTTTGTGGTGAAGGATTACGGGCACTTGGATCTGTTGGACGATGAGACTGGAGGAATCAGAGGGAAGGCGAGTTATTGTCTGTGTAAGAATGGCGAATCCAGAGAGCCGATGCGGCGGTTTGTTGGTGGGGCTGTGGTGGCGTTTCTGAAGGCTTATTTGGATGGAGAAGATGAAGATTTGAGTGGCATTGTAGATGGGGATTTGAGCTTGCCTGTTCAACTTCAGACTGTTGAATCTCTTCTTTGA

Protein sequence

MAMASFSFPPPSATAGSATSPNVFEIGKFNAVLEKVDSGFCSSGRYLPVPPPKPLLIGRPSDPGEFPVLLLIHGYLLYNTFYSQLIQQIASHGFIVIAPQLYTLAGPDTSEEIKATAAIINWLSEGLRQLLPPHVNPNLNKIALAGHSRGGKTSFALAQHKSIPKSPKFSALIGLDPVDGMGSGKQTHPPVLKYIPQSFDFELPVLVIGSGLGEFKRNPLFPPCAPKGINHEDFFNECRSPAHYFVVKDYGHLDLLDDETGGIRGKASYCLCKNGESREPMRRFVGGAVVAFLKAYLDGEDEDLSGIVDGDLSLPVQLQTVESLL
Homology
BLAST of Lcy01g002640 vs. ExPASy Swiss-Prot
Match: Q9M7I7 (Chlorophyllase-2 OS=Arabidopsis thaliana OX=3702 GN=CLH2 PE=1 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 1.0e-107
Identity = 190/317 (59.94%), Postives = 238/317 (75.08%), Query Frame = 0

Query: 17  SATSPNVFEIGKFNAVLEKVDSG-----FCSSGRYLPVPPPKPLLIGRPSDPGEFPVLLL 76
           S++S N FE GK+ + L  +DS         S R  P  PPK LL+  P + G++PV++L
Sbjct: 3   SSSSRNAFEDGKYKSNLLTLDSSSRCCKITPSSRASP-SPPKQLLVATPVEEGDYPVVML 62

Query: 77  IHGYLLYNTFYSQLIQQIASHGFIVIAPQLYTLAGPDTSEEIKATAAIINWLSEGLRQLL 136
           +HGYLLYN+FYSQL+  ++SHGFI+IAPQLY++AGPDT +EIK+TA I++WLS GL   L
Sbjct: 63  LHGYLLYNSFYSQLMLHVSSHGFILIAPQLYSIAGPDTMDEIKSTAEIMDWLSVGLNHFL 122

Query: 137 PPHVNPNLNKIALAGHSRGGKTSFALAQHK-SIPKSPKFSALIGLDPVDGMGSGKQTHPP 196
           P  V PNL+K AL+GHSRGGKT+FA+A  K     + K S LIG+DPVDG G GKQT PP
Sbjct: 123 PAQVTPNLSKFALSGHSRGGKTAFAVALKKFGYSSNLKISTLIGIDPVDGTGKGKQTPPP 182

Query: 197 VLKYIPQSFDFE-LPVLVIGSGLGEFKRNPLFPPCAPKGINHEDFFNECRSPAHYFVVKD 256
           VL Y+P SFD +  P+LVIGSGLGE  RNPLFPPCAP G+NH +FF EC+ PA +FV KD
Sbjct: 183 VLAYLPNSFDLDKTPILVIGSGLGETARNPLFPPCAPPGVNHREFFRECQGPAWHFVAKD 242

Query: 257 YGHLDLLDDETGGIRGKASYCLCKNGESREPMRRFVGGAVVAFLKAYLDGEDEDLSGIVD 316
           YGHLD+LDD+T GIRGK+SYCLCKNGE R PMRRFVGG VV+FLKAYL+G+D +L  I D
Sbjct: 243 YGHLDMLDDDTKGIRGKSSYCLCKNGEERRPMRRFVGGLVVSFLKAYLEGDDRELVKIKD 302

Query: 317 G-DLSLPVQLQTVESLL 326
           G    +PV++Q  E ++
Sbjct: 303 GCHEDVPVEIQEFEVIM 318

BLAST of Lcy01g002640 vs. ExPASy Swiss-Prot
Match: O22527 (Chlorophyllase-1 OS=Arabidopsis thaliana OX=3702 GN=CLH1 PE=1 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 8.0e-68
Identity = 138/310 (44.52%), Postives = 183/310 (59.03%), Query Frame = 0

Query: 12  SATAGSATSPNVFEIGKFNAVLEKVDSGFCSSGRYLPVPPPKPLLIGRPSDPGEFPVLLL 71
           S T  S  +P  FEIG        VD     S       PPKP+ I  P+  G +PV+L 
Sbjct: 7   SPTFSSVVTPAAFEIGSLPTTEIPVDPVENDS-----TAPPKPVRITCPTVAGTYPVVLF 66

Query: 72  IHGYLLYNTFYSQLIQQIASHGFIVIAPQLYTLAGPDTSEEIKATAAIINWLSEGLRQLL 131
            HG+ L N FYS ++  IASHG+I++APQL  L  P    E+    ++INW SE L+  L
Sbjct: 67  FHGFYLRNYFYSDVLNHIASHGYILVAPQLCKLLPPGGQVEVDDAGSVINWASENLKAHL 126

Query: 132 PPHVNPNLNKIALAGHSRGGKTSFALAQHKSIPKSPK--FSALIGLDPVDGMGSGKQTHP 191
           P  VN N    +L GHSRGGKT+FA+A   +    P   FSALIG+DPV G     +T P
Sbjct: 127 PTSVNANGKYTSLVGHSRGGKTAFAVALGHAATLDPSITFSALIGIDPVAGTNKYIRTDP 186

Query: 192 PVLKYIPQSFDFELPVLVIGSGLGEFKRNPLFPPCAPKGINHEDFFNECRSPAHYFVVKD 251
            +L Y P+SF+ ++PV V+G+GLG  K N + PPCAP  +NHE+F+ EC++   +FV  D
Sbjct: 187 HILTYKPESFELDIPVAVVGTGLGP-KWNNVMPPCAPTDLNHEEFYKECKATKAHFVAAD 246

Query: 252 YGHLDLLDDETGGIRGKASYCLCKNGE-SREPMRRFVGGAVVAFLKAYLDGEDEDLSGIV 311
           YGH+D+LDD+  G  G  + C+CKNG+  +  MR FVGG VVAFLK  L GE  ++  IV
Sbjct: 247 YGHMDMLDDDLPGFVGFMAGCMCKNGQRKKSEMRSFVGGIVVAFLKYSLWGEKAEIRLIV 306

Query: 312 DGDLSLPVQL 319
                 P +L
Sbjct: 307 KDPSVSPAKL 310

BLAST of Lcy01g002640 vs. ExPASy Swiss-Prot
Match: Q94LX1 (Chlorophyllase-1, chloroplastic OS=Citrus unshiu OX=55188 PE=2 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 4.1e-64
Identity = 129/277 (46.57%), Postives = 174/277 (62.82%), Query Frame = 0

Query: 50  PPPKPLLIGRPSDPGEFPVLLLIHGYLLYNTFYSQLIQQIASHGFIVIAPQLYT-LAGPD 109
           PPPKPL+I  P++ G F V+L +HG  L N  YS++   IASHGFIV+APQLYT +  P 
Sbjct: 48  PPPKPLIIVTPAEKGTFNVILFLHGTSLSNKSYSKIFDHIASHGFIVVAPQLYTSIPPPS 107

Query: 110 TSEEIKATAAIINWLSEGLRQLLPPHVNPNLNKIALAGHSRGGKTSFALAQHKSIPKSPK 169
            + E+ + A +  WL +GL+Q LP +   N++ +A+ GHSRGG+T+FAL+          
Sbjct: 108 ATNELNSAAEVAEWLPQGLQQNLPENTEANVSLVAVMGHSRGGQTAFALSLRYG------ 167

Query: 170 FSALIGLDPVDGMGSGKQTHPPVLKYIPQSFDFELPVLVIGSGLGEFKRNPLFPPCAPKG 229
           F A+IGLDPV G        P +L +   SFDF +PV VIG+GLG   R      CAP+G
Sbjct: 168 FGAVIGLDPVAGTSKTTGLDPSILSF--DSFDFSIPVTVIGTGLGGVAR--CITACAPEG 227

Query: 230 INHEDFFNECRSPAH-YFVVKDYGHLDLLDDETGGIRGKA-SYCLCKNG-ESREPMRRFV 289
            NHE+FFN C++ +  +FV  DYGH+D+LDD    ++  A S   CKNG ESR+PMRR V
Sbjct: 228 ANHEEFFNRCKNSSRAHFVATDYGHMDILDDNPSDVKSWALSKYFCKNGNESRDPMRRCV 287

Query: 290 GGAVVAFLKAYLDGEDEDLSGIVDGDLSLPVQLQTVE 323
            G VVAFLK + DG+ ED   I+      P++L +VE
Sbjct: 288 SGIVVAFLKDFFDGDAEDFRQILKDPSFAPIKLDSVE 314

BLAST of Lcy01g002640 vs. ExPASy Swiss-Prot
Match: Q9MV14 (Chlorophyllase-1, chloroplastic OS=Citrus sinensis OX=2711 GN=CHLASE1 PE=1 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 1.0e-62
Identity = 128/277 (46.21%), Postives = 172/277 (62.09%), Query Frame = 0

Query: 50  PPPKPLLIGRPSDPGEFPVLLLIHGYLLYNTFYSQLIQQIASHGFIVIAPQLYT-LAGPD 109
           PPPKPL+I  P+  G F V+L +HG  L N  YS++   IASHGFIV+APQLYT +  P 
Sbjct: 48  PPPKPLIIVTPAGKGTFNVILFLHGTSLSNKSYSKIFDHIASHGFIVVAPQLYTSIPPPS 107

Query: 110 TSEEIKATAAIINWLSEGLRQLLPPHVNPNLNKIALAGHSRGGKTSFALAQHKSIPKSPK 169
            + E+ + A +  WL +GL+Q LP +   N++ +A+ GHSRGG+T+FAL+          
Sbjct: 108 ATNELNSAAEVAEWLPQGLQQNLPENTEANVSLVAVMGHSRGGQTAFALSLRYG------ 167

Query: 170 FSALIGLDPVDGMGSGKQTHPPVLKYIPQSFDFELPVLVIGSGLGEFKRNPLFPPCAPKG 229
           F A+IGLDPV G        P +L +   SFDF +PV VIG+GLG   R      CAP+G
Sbjct: 168 FGAVIGLDPVAGTSKTTGLDPSILSF--DSFDFSIPVTVIGTGLGGVAR--CITACAPEG 227

Query: 230 INHEDFFNECRSPAH-YFVVKDYGHLDLLDDETGGIRGKA-SYCLCKNG-ESREPMRRFV 289
            NHE+FFN C++ +  +FV  DYGH+D+LDD    ++  A S   CKNG ESR+PMRR V
Sbjct: 228 ANHEEFFNRCKNSSRAHFVATDYGHMDILDDNPSDVKSWALSKYFCKNGNESRDPMRRCV 287

Query: 290 GGAVVAFLKAYLDGEDEDLSGIVDGDLSLPVQLQTVE 323
            G VVAFLK +  G+ ED   I+      P++L +VE
Sbjct: 288 SGIVVAFLKDFFYGDAEDFRQILKDPSFAPIKLDSVE 314

BLAST of Lcy01g002640 vs. ExPASy Swiss-Prot
Match: Q9LE89 (Chlorophyllase type 0 OS=Chenopodium album OX=3559 GN=CACLH PE=1 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 4.9e-49
Identity = 110/275 (40.00%), Postives = 157/275 (57.09%), Query Frame = 0

Query: 52  PKPLLIGRPSDPGEFPVLLLIHGYLLYNTFYSQLIQQIASHGFIVIAPQLYTLAG---PD 111
           P+PL+I  P + G +PVLL IHG +L N  YS     IASHGFIV+AP+L+ L     P 
Sbjct: 60  PEPLIIISPKEAGVYPVLLFIHGTMLSNEDYSLFFNYIASHGFIVVAPKLFRLFPPKLPS 119

Query: 112 TSEEIKATAAIINWLSEGLRQLLPPH---VNPNLNKIALAGHSRGGKTSFALA-QHKSIP 171
             +EI   A++ NW+   L+ +L  +   V  +L K+A++GHSRGGK++FALA    +I 
Sbjct: 120 QQDEIDMAASVANWMPLYLQVVLQRYVTGVEGDLEKLAISGHSRGGKSAFALALGFSNIK 179

Query: 172 KSPKFSALIGLDPVDGMGSGKQTHPPVLKYIPQSFDFELPVLVIGSGLGEFKRNPLFPPC 231
               FSALIG+DPV G     +T P VL Y P SF+  +PV VIGSGLG    +     C
Sbjct: 180 LDVTFSALIGVDPVAGRSVDDRTLPHVLTYKPNSFNLSIPVTVIGSGLGNHTIS-----C 239

Query: 232 APKGINHEDFFNECRSPAHYFVVKDYGHLDLLDD-ETGGIRGKASYCLCKNGESREPMRR 291
           AP  ++H+ F++EC+  + +FV+  YGH+D+L++     I    S    ++   +  MRR
Sbjct: 240 APNHVSHQQFYDECKENSSHFVITKYGHMDMLNEFRLSPIAVTMSLMCAQSFRPKATMRR 299

Query: 292 FVGGAVVAFLKAYLDGEDEDLSGIVDGDLSLPVQL 319
            +GG +VAFL AY   +      I+      P  L
Sbjct: 300 TLGGIMVAFLNAYFRDDGRQYYAIIANRSLAPTNL 329

BLAST of Lcy01g002640 vs. ExPASy TrEMBL
Match: A0A6J1H5Z8 (Chlorophyllase OS=Cucurbita moschata OX=3662 GN=LOC111459924 PE=3 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 2.2e-161
Identity = 284/326 (87.12%), Postives = 297/326 (91.10%), Query Frame = 0

Query: 1   MAMASFSFPPPSATAGSATSPNVFEIGKFNAVLEKVDS-GFCSSGRYLPVPPPKPLLIGR 60
           MAMA+ SFPPPSATA SATSP VFEIGKFNAVLEKV+S G CSSGR+LP PPPKPLLI R
Sbjct: 1   MAMATASFPPPSATAASATSPIVFEIGKFNAVLEKVESAGCCSSGRHLPPPPPKPLLIAR 60

Query: 61  PSDPGEFPVLLLIHGYLLYNTFYSQLIQQIASHGFIVIAPQLYTLAGPDTSEEIKATAAI 120
           PSD GEFPVLLLIHGYLLYNTFYSQLI QIASHGFIVIAPQLY++AGPDTSEEIKATAA+
Sbjct: 61  PSDAGEFPVLLLIHGYLLYNTFYSQLIHQIASHGFIVIAPQLYSVAGPDTSEEIKATAAV 120

Query: 121 INWLSEGLRQLLPPHVNPNLNKIALAGHSRGGKTSFALAQHKSIPKSPKFSALIGLDPVD 180
           INWL EGLRQ LPPHVNPNLNKIAL+GHSRGGKTSFALA   +  KSPK SALIGLDPVD
Sbjct: 121 INWLPEGLRQHLPPHVNPNLNKIALSGHSRGGKTSFALALALAEQKSPKLSALIGLDPVD 180

Query: 181 GMGSGKQTHPPVLKYIPQSFDFELPVLVIGSGLGEFKRNPLFPPCAPKGINHEDFFNECR 240
           G GSGKQTHPPVLKYIPQSFD  +PVLVIGSGLGE KRNPLFPPCAPKGINHE+FF ECR
Sbjct: 181 GTGSGKQTHPPVLKYIPQSFDLGVPVLVIGSGLGELKRNPLFPPCAPKGINHEEFFEECR 240

Query: 241 SPAHYFVVKDYGHLDLLDDETGGIRGKASYCLCKNGESREPMRRFVGGAVVAFLKAYLDG 300
           +PAHYFV KDYGHLDLLDDETGG+RGKASYCLCKNGESREPMRRFVGGAVVAFLKAYL+G
Sbjct: 241 NPAHYFVAKDYGHLDLLDDETGGLRGKASYCLCKNGESREPMRRFVGGAVVAFLKAYLNG 300

Query: 301 EDEDLSGIVDGDLSLPVQLQTVESLL 326
           ED DL  I DGDL LPV LQTVESLL
Sbjct: 301 EDGDLRAIEDGDLCLPVHLQTVESLL 326

BLAST of Lcy01g002640 vs. ExPASy TrEMBL
Match: A0A6J1KYL8 (Chlorophyllase OS=Cucurbita maxima OX=3661 GN=LOC111499382 PE=3 SV=1)

HSP 1 Score: 573.5 bits (1477), Expect = 5.4e-160
Identity = 283/326 (86.81%), Postives = 296/326 (90.80%), Query Frame = 0

Query: 1   MAMASFSFPPPSATAGSATSPNVFEIGKFNAVLEKVDS-GFCSSGRYLPVPPPKPLLIGR 60
           MAMA+ SFPPPSATA SATS  VFEIGKFNAVLEKV+S G CSSGR+LP PPPKPLLIGR
Sbjct: 1   MAMATASFPPPSATAFSATSSIVFEIGKFNAVLEKVESAGCCSSGRHLPAPPPKPLLIGR 60

Query: 61  PSDPGEFPVLLLIHGYLLYNTFYSQLIQQIASHGFIVIAPQLYTLAGPDTSEEIKATAAI 120
           PS  GEFPVLLLIHGYLLYNTFYSQLI QIASHGFIVIAPQLY++AGPDTSEEIKATAA+
Sbjct: 61  PSIAGEFPVLLLIHGYLLYNTFYSQLIHQIASHGFIVIAPQLYSVAGPDTSEEIKATAAV 120

Query: 121 INWLSEGLRQLLPPHVNPNLNKIALAGHSRGGKTSFALAQHKSIPKSPKFSALIGLDPVD 180
           INWL EGLRQ LPPHVNPNLNKIAL+GHSRGGKTSFALA   +  KSPK SALIGLDPVD
Sbjct: 121 INWLPEGLRQHLPPHVNPNLNKIALSGHSRGGKTSFALALALAEQKSPKLSALIGLDPVD 180

Query: 181 GMGSGKQTHPPVLKYIPQSFDFELPVLVIGSGLGEFKRNPLFPPCAPKGINHEDFFNECR 240
           G GSGKQTHPPVLKYIPQSFD  LPVLVIGSGLGE KRNPLFPPCAPKGINHE+FF ECR
Sbjct: 181 GTGSGKQTHPPVLKYIPQSFDLGLPVLVIGSGLGELKRNPLFPPCAPKGINHEEFFKECR 240

Query: 241 SPAHYFVVKDYGHLDLLDDETGGIRGKASYCLCKNGESREPMRRFVGGAVVAFLKAYLDG 300
           +PAHYFV KDYGHLDLLDDETGG+RGKASYCLCKNG+SREPMRRFVGGAVVAFLKAYL+G
Sbjct: 241 NPAHYFVAKDYGHLDLLDDETGGLRGKASYCLCKNGDSREPMRRFVGGAVVAFLKAYLNG 300

Query: 301 EDEDLSGIVDGDLSLPVQLQTVESLL 326
           ED DL  I DGDL LPV LQTVESLL
Sbjct: 301 EDGDLRAIEDGDLCLPVHLQTVESLL 326

BLAST of Lcy01g002640 vs. ExPASy TrEMBL
Match: A0A6J1C9F9 (Chlorophyllase OS=Momordica charantia OX=3673 GN=LOC111009609 PE=3 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 1.2e-151
Identity = 269/329 (81.76%), Postives = 294/329 (89.36%), Query Frame = 0

Query: 1   MAMASFSFPPPSATAGSATSPNVFEIGKFNAVLEKVDSGFCSSGRYLPVPPPKPLLIGRP 60
           MAMASFSF PPS TAGSAT+ NVFE+G FNAV+EKV+SGFC  GR+    PPKP++IGRP
Sbjct: 1   MAMASFSFLPPSVTAGSATAANVFEMGSFNAVMEKVESGFCGGGRF---APPKPVVIGRP 60

Query: 61  SDPGEFPVLLLIHGYLLYNTFYSQLIQQIASHGFIVIAPQLYTLAGPDTSEEIKATAAII 120
           SD GEFPVLLLIHGYLLYNTFYSQLIQ IASHGFIV+APQLYT+AGPD+S EIKATAAI 
Sbjct: 61  SDGGEFPVLLLIHGYLLYNTFYSQLIQHIASHGFIVVAPQLYTVAGPDSSGEIKATAAIA 120

Query: 121 NWLSEGLRQLLPPHVNPNLNKIALAGHSRGGKTS--FALAQHKSIPKSPKFSALIGLDPV 180
           +WL EGLRQ LPP+VNPNL+K+ALAGHSRGGKTS  FALA +KSI  + KFSALIGLDPV
Sbjct: 121 DWLPEGLRQHLPPNVNPNLDKLALAGHSRGGKTSFAFALALNKSITTALKFSALIGLDPV 180

Query: 181 DGMGSGKQTHPPVLKYIPQSFDF--ELPVLVIGSGLGEFKRNPLFPPCAPKGINHEDFFN 240
           DG GSGKQTHPPVLK++PQSFDF   LPVLVIGSGLGE KRNPLFPPCAPKG+NHEDFFN
Sbjct: 181 DGTGSGKQTHPPVLKFVPQSFDFGLALPVLVIGSGLGEVKRNPLFPPCAPKGLNHEDFFN 240

Query: 241 ECRSPAHYFVVKDYGHLDLLDDETGGIRGKASYCLCKNGESREPMRRFVGGAVVAFLKAY 300
           ECR+PAHY VVKDYGHLDLLDDETGGIRGKASYCLC+NGESR PMRRFVGGAVVAFLKAY
Sbjct: 241 ECRNPAHYLVVKDYGHLDLLDDETGGIRGKASYCLCRNGESRGPMRRFVGGAVVAFLKAY 300

Query: 301 LDGEDEDLSGIVDGDLSLPVQLQTVESLL 326
           LDGE+ DL+ I DG  SLP+ LQTV+SLL
Sbjct: 301 LDGEEGDLAAIEDGSRSLPLDLQTVKSLL 326

BLAST of Lcy01g002640 vs. ExPASy TrEMBL
Match: A0A5A7UQQ1 (Chlorophyllase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G001310 PE=3 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 2.5e-149
Identity = 266/326 (81.60%), Postives = 285/326 (87.42%), Query Frame = 0

Query: 1   MAMASFSFPPPSATAGSATSPNVFEIGKFNAVLEKVD-SGFCSSGRYLPVPPPKPLLIGR 60
           MAMA+ SFPPP  TAGSAT  NVFEIGKFNAVLEKV+  G CSSGR+L   PPKPLLIGR
Sbjct: 1   MAMATASFPPPLPTAGSATLSNVFEIGKFNAVLEKVEPGGCCSSGRFL---PPKPLLIGR 60

Query: 61  PSDPGEFPVLLLIHGYLLYNTFYSQLIQQIASHGFIVIAPQLYTLAGPDTSEEIKATAAI 120
           PS+ GEFPVLLL+HGYLLYNTFYSQLI  IASHGFIV+APQLYT+AGPD SEEIKATAA+
Sbjct: 61  PSEAGEFPVLLLVHGYLLYNTFYSQLIHHIASHGFIVVAPQLYTVAGPDASEEIKATAAV 120

Query: 121 INWLSEGLRQLLPPHVNPNLNKIALAGHSRGGKTSFALAQHKSIPKSPKFSALIGLDPVD 180
           INWL  GLRQ LPPHVNPNL KIALAGHSRGGKTSFALA    + KSPK SALIGLDPVD
Sbjct: 121 INWLPTGLRQHLPPHVNPNLTKIALAGHSRGGKTSFALA----LQKSPKLSALIGLDPVD 180

Query: 181 GMGSGKQTHPPVLKYIPQSFDFELPVLVIGSGLGEFKRNPLFPPCAPKGINHEDFFNECR 240
           G GSGKQTHPPVLKYIPQS D  +PVLVIGSGLGE K NPLFPPCAPKGINHE+FF ECR
Sbjct: 181 GTGSGKQTHPPVLKYIPQSLDLGIPVLVIGSGLGELKMNPLFPPCAPKGINHEEFFKECR 240

Query: 241 SPAHYFVVKDYGHLDLLDDETGGIRGKASYCLCKNGESREPMRRFVGGAVVAFLKAYLDG 300
           +PA++FVVKDYGH+DLLDDETGGIRGK SYCLCKNGESREPMRRFVGGAVVAFLK+ L+G
Sbjct: 241 NPAYHFVVKDYGHMDLLDDETGGIRGKVSYCLCKNGESREPMRRFVGGAVVAFLKSCLNG 300

Query: 301 EDEDLSGIVDGDLSLPVQLQTVESLL 326
           E+ DL  I  GDL LPV LQT+ESLL
Sbjct: 301 EEGDLKAIEGGDLILPVHLQTIESLL 319

BLAST of Lcy01g002640 vs. ExPASy TrEMBL
Match: A0A1S3BME1 (Chlorophyllase OS=Cucumis melo OX=3656 GN=LOC103491233 PE=3 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 2.5e-149
Identity = 266/326 (81.60%), Postives = 285/326 (87.42%), Query Frame = 0

Query: 1   MAMASFSFPPPSATAGSATSPNVFEIGKFNAVLEKVD-SGFCSSGRYLPVPPPKPLLIGR 60
           MAMA+ SFPPP  TAGSAT  NVFEIGKFNAVLEKV+  G CSSGR+L   PPKPLLIGR
Sbjct: 1   MAMATASFPPPLPTAGSATLSNVFEIGKFNAVLEKVEPGGCCSSGRFL---PPKPLLIGR 60

Query: 61  PSDPGEFPVLLLIHGYLLYNTFYSQLIQQIASHGFIVIAPQLYTLAGPDTSEEIKATAAI 120
           PS+ GEFPVLLL+HGYLLYNTFYSQLI  IASHGFIV+APQLYT+AGPD SEEIKATAA+
Sbjct: 61  PSEAGEFPVLLLVHGYLLYNTFYSQLIHHIASHGFIVVAPQLYTVAGPDASEEIKATAAV 120

Query: 121 INWLSEGLRQLLPPHVNPNLNKIALAGHSRGGKTSFALAQHKSIPKSPKFSALIGLDPVD 180
           INWL  GLRQ LPPHVNPNL KIALAGHSRGGKTSFALA    + KSPK SALIGLDPVD
Sbjct: 121 INWLPTGLRQHLPPHVNPNLTKIALAGHSRGGKTSFALA----LQKSPKLSALIGLDPVD 180

Query: 181 GMGSGKQTHPPVLKYIPQSFDFELPVLVIGSGLGEFKRNPLFPPCAPKGINHEDFFNECR 240
           G GSGKQTHPPVLKYIPQS D  +PVLVIGSGLGE K NPLFPPCAPKGINHE+FF ECR
Sbjct: 181 GTGSGKQTHPPVLKYIPQSLDLGIPVLVIGSGLGELKMNPLFPPCAPKGINHEEFFKECR 240

Query: 241 SPAHYFVVKDYGHLDLLDDETGGIRGKASYCLCKNGESREPMRRFVGGAVVAFLKAYLDG 300
           +PA++FVVKDYGH+DLLDDETGGIRGK SYCLCKNGESREPMRRFVGGAVVAFLK+ L+G
Sbjct: 241 NPAYHFVVKDYGHMDLLDDETGGIRGKVSYCLCKNGESREPMRRFVGGAVVAFLKSCLNG 300

Query: 301 EDEDLSGIVDGDLSLPVQLQTVESLL 326
           E+ DL  I  GDL LPV LQT+ESLL
Sbjct: 301 EEGDLKAIEGGDLILPVHLQTIESLL 319

BLAST of Lcy01g002640 vs. NCBI nr
Match: KAG7013529.1 (Chlorophyllase-2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 578.6 bits (1490), Expect = 3.5e-161
Identity = 284/326 (87.12%), Postives = 297/326 (91.10%), Query Frame = 0

Query: 1   MAMASFSFPPPSATAGSATSPNVFEIGKFNAVLEKVDS-GFCSSGRYLPVPPPKPLLIGR 60
           MAMA+ SFPPPSATA SATSP VFEIGKFNAVLEKV+S G CSSGR+LP PPPKPLLIGR
Sbjct: 1   MAMATASFPPPSATAASATSPIVFEIGKFNAVLEKVESAGCCSSGRHLPPPPPKPLLIGR 60

Query: 61  PSDPGEFPVLLLIHGYLLYNTFYSQLIQQIASHGFIVIAPQLYTLAGPDTSEEIKATAAI 120
           PSD GEFPVLLLIHGYLLYNTFYSQLI QIASHGFIVIAPQLY++AGPDTSEEIKATAA+
Sbjct: 61  PSDAGEFPVLLLIHGYLLYNTFYSQLIHQIASHGFIVIAPQLYSVAGPDTSEEIKATAAV 120

Query: 121 INWLSEGLRQLLPPHVNPNLNKIALAGHSRGGKTSFALAQHKSIPKSPKFSALIGLDPVD 180
           INWL EGLRQ LPPHVNPNLNKIAL+GHSRGGKTSFALA   +  KSPK SALIGLDPVD
Sbjct: 121 INWLPEGLRQHLPPHVNPNLNKIALSGHSRGGKTSFALALALAEQKSPKLSALIGLDPVD 180

Query: 181 GMGSGKQTHPPVLKYIPQSFDFELPVLVIGSGLGEFKRNPLFPPCAPKGINHEDFFNECR 240
           G GSGKQTHPPVLKYIP SFD  +PVLVIGSGLGE KRNPLFPPCAPKGINHE+FF ECR
Sbjct: 181 GTGSGKQTHPPVLKYIPHSFDLGVPVLVIGSGLGELKRNPLFPPCAPKGINHEEFFEECR 240

Query: 241 SPAHYFVVKDYGHLDLLDDETGGIRGKASYCLCKNGESREPMRRFVGGAVVAFLKAYLDG 300
           +PAHYFV KDYGHLDLLDDETGG+RGKASYCLCKNGESREPMRRFVGGAVVAFLKAYL+G
Sbjct: 241 NPAHYFVAKDYGHLDLLDDETGGLRGKASYCLCKNGESREPMRRFVGGAVVAFLKAYLNG 300

Query: 301 EDEDLSGIVDGDLSLPVQLQTVESLL 326
           ED DL  I DGDL LPV LQTVESLL
Sbjct: 301 EDGDLRAIEDGDLCLPVHLQTVESLL 326

BLAST of Lcy01g002640 vs. NCBI nr
Match: XP_022958739.1 (chlorophyllase-2, chloroplastic [Cucurbita moschata])

HSP 1 Score: 578.2 bits (1489), Expect = 4.5e-161
Identity = 284/326 (87.12%), Postives = 297/326 (91.10%), Query Frame = 0

Query: 1   MAMASFSFPPPSATAGSATSPNVFEIGKFNAVLEKVDS-GFCSSGRYLPVPPPKPLLIGR 60
           MAMA+ SFPPPSATA SATSP VFEIGKFNAVLEKV+S G CSSGR+LP PPPKPLLI R
Sbjct: 1   MAMATASFPPPSATAASATSPIVFEIGKFNAVLEKVESAGCCSSGRHLPPPPPKPLLIAR 60

Query: 61  PSDPGEFPVLLLIHGYLLYNTFYSQLIQQIASHGFIVIAPQLYTLAGPDTSEEIKATAAI 120
           PSD GEFPVLLLIHGYLLYNTFYSQLI QIASHGFIVIAPQLY++AGPDTSEEIKATAA+
Sbjct: 61  PSDAGEFPVLLLIHGYLLYNTFYSQLIHQIASHGFIVIAPQLYSVAGPDTSEEIKATAAV 120

Query: 121 INWLSEGLRQLLPPHVNPNLNKIALAGHSRGGKTSFALAQHKSIPKSPKFSALIGLDPVD 180
           INWL EGLRQ LPPHVNPNLNKIAL+GHSRGGKTSFALA   +  KSPK SALIGLDPVD
Sbjct: 121 INWLPEGLRQHLPPHVNPNLNKIALSGHSRGGKTSFALALALAEQKSPKLSALIGLDPVD 180

Query: 181 GMGSGKQTHPPVLKYIPQSFDFELPVLVIGSGLGEFKRNPLFPPCAPKGINHEDFFNECR 240
           G GSGKQTHPPVLKYIPQSFD  +PVLVIGSGLGE KRNPLFPPCAPKGINHE+FF ECR
Sbjct: 181 GTGSGKQTHPPVLKYIPQSFDLGVPVLVIGSGLGELKRNPLFPPCAPKGINHEEFFEECR 240

Query: 241 SPAHYFVVKDYGHLDLLDDETGGIRGKASYCLCKNGESREPMRRFVGGAVVAFLKAYLDG 300
           +PAHYFV KDYGHLDLLDDETGG+RGKASYCLCKNGESREPMRRFVGGAVVAFLKAYL+G
Sbjct: 241 NPAHYFVAKDYGHLDLLDDETGGLRGKASYCLCKNGESREPMRRFVGGAVVAFLKAYLNG 300

Query: 301 EDEDLSGIVDGDLSLPVQLQTVESLL 326
           ED DL  I DGDL LPV LQTVESLL
Sbjct: 301 EDGDLRAIEDGDLCLPVHLQTVESLL 326

BLAST of Lcy01g002640 vs. NCBI nr
Match: KAG6574961.1 (Chlorophyllase-2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 577.0 bits (1486), Expect = 1.0e-160
Identity = 283/326 (86.81%), Postives = 296/326 (90.80%), Query Frame = 0

Query: 1   MAMASFSFPPPSATAGSATSPNVFEIGKFNAVLEKVDS-GFCSSGRYLPVPPPKPLLIGR 60
           MAMA+ SFPPPSATA SATSP VFEIGKFNAVLEKV+S G CSSGR+LP PPPKPLLIGR
Sbjct: 1   MAMATASFPPPSATAASATSPIVFEIGKFNAVLEKVESAGCCSSGRHLPPPPPKPLLIGR 60

Query: 61  PSDPGEFPVLLLIHGYLLYNTFYSQLIQQIASHGFIVIAPQLYTLAGPDTSEEIKATAAI 120
           PSD GEFPVLLLIHGYLLYNTFYSQLI QIASHGFIVIAPQLY++AGPDTSEEIKATAA+
Sbjct: 61  PSDAGEFPVLLLIHGYLLYNTFYSQLIHQIASHGFIVIAPQLYSVAGPDTSEEIKATAAV 120

Query: 121 INWLSEGLRQLLPPHVNPNLNKIALAGHSRGGKTSFALAQHKSIPKSPKFSALIGLDPVD 180
           INWL EGLRQ LPPHVNPNLNKIAL+GHSRGGKTSFALA   +  KSPK SALIGLDPVD
Sbjct: 121 INWLPEGLRQHLPPHVNPNLNKIALSGHSRGGKTSFALALALAEQKSPKLSALIGLDPVD 180

Query: 181 GMGSGKQTHPPVLKYIPQSFDFELPVLVIGSGLGEFKRNPLFPPCAPKGINHEDFFNECR 240
           G GSGKQTHPPVLKYIP SFD  +PVLVIGSGLGE KRNPLFPPCAPKGINHE+FF ECR
Sbjct: 181 GTGSGKQTHPPVLKYIPHSFDLGVPVLVIGSGLGELKRNPLFPPCAPKGINHEEFFEECR 240

Query: 241 SPAHYFVVKDYGHLDLLDDETGGIRGKASYCLCKNGESREPMRRFVGGAVVAFLKAYLDG 300
           +PAHYFV KDYGHLDLLDDETGG+RGKASYCLCKNGESREPMRRFVGGAVVAFLKAYL+G
Sbjct: 241 NPAHYFVAKDYGHLDLLDDETGGLRGKASYCLCKNGESREPMRRFVGGAVVAFLKAYLNG 300

Query: 301 EDEDLSGIVDGDLSLPVQLQTVESLL 326
           ED DL    DGDL LPV LQTVESLL
Sbjct: 301 EDGDLRAFEDGDLCLPVHLQTVESLL 326

BLAST of Lcy01g002640 vs. NCBI nr
Match: XP_023548652.1 (chlorophyllase-2, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 575.1 bits (1481), Expect = 3.8e-160
Identity = 283/326 (86.81%), Postives = 296/326 (90.80%), Query Frame = 0

Query: 1   MAMASFSFPPPSATAGSATSPNVFEIGKFNAVLEKVDS-GFCSSGRYLPVPPPKPLLIGR 60
           MAMA+ SFPPPSATA SATS  VFEIGKFNAVLEKV+S G CSSGR+LP PPPKPLLIGR
Sbjct: 1   MAMATASFPPPSATAASATSSIVFEIGKFNAVLEKVESAGCCSSGRHLPAPPPKPLLIGR 60

Query: 61  PSDPGEFPVLLLIHGYLLYNTFYSQLIQQIASHGFIVIAPQLYTLAGPDTSEEIKATAAI 120
           PSD GEF VLLLIHGYLLYNTFYSQLI QIASHGFIVIAPQLY++AGPDTSEEIKATAA+
Sbjct: 61  PSDAGEFQVLLLIHGYLLYNTFYSQLIHQIASHGFIVIAPQLYSVAGPDTSEEIKATAAV 120

Query: 121 INWLSEGLRQLLPPHVNPNLNKIALAGHSRGGKTSFALAQHKSIPKSPKFSALIGLDPVD 180
           INWL EGLRQ LPPHVNPNLNKIAL+GHSRGGKTSFALA   +  KSPK SALIGLDPVD
Sbjct: 121 INWLPEGLRQHLPPHVNPNLNKIALSGHSRGGKTSFALALALAEQKSPKLSALIGLDPVD 180

Query: 181 GMGSGKQTHPPVLKYIPQSFDFELPVLVIGSGLGEFKRNPLFPPCAPKGINHEDFFNECR 240
           G GSGKQTHPPVLKYIPQSFD  +PVLVIGSGLGE KRNPLFPPCAPKGINHE+FF ECR
Sbjct: 181 GTGSGKQTHPPVLKYIPQSFDLGVPVLVIGSGLGELKRNPLFPPCAPKGINHEEFFEECR 240

Query: 241 SPAHYFVVKDYGHLDLLDDETGGIRGKASYCLCKNGESREPMRRFVGGAVVAFLKAYLDG 300
           +PAHYFV KDYGHLDLLDDETGG+RGKASYCLCKNGESREPMRRFVGGAVVAFLKAYL+G
Sbjct: 241 NPAHYFVAKDYGHLDLLDDETGGLRGKASYCLCKNGESREPMRRFVGGAVVAFLKAYLNG 300

Query: 301 EDEDLSGIVDGDLSLPVQLQTVESLL 326
           ED DL  I DGDL LPV LQTVESLL
Sbjct: 301 EDGDLRAIEDGDLCLPVHLQTVESLL 326

BLAST of Lcy01g002640 vs. NCBI nr
Match: XP_023006741.1 (chlorophyllase-2, chloroplastic [Cucurbita maxima])

HSP 1 Score: 573.5 bits (1477), Expect = 1.1e-159
Identity = 283/326 (86.81%), Postives = 296/326 (90.80%), Query Frame = 0

Query: 1   MAMASFSFPPPSATAGSATSPNVFEIGKFNAVLEKVDS-GFCSSGRYLPVPPPKPLLIGR 60
           MAMA+ SFPPPSATA SATS  VFEIGKFNAVLEKV+S G CSSGR+LP PPPKPLLIGR
Sbjct: 1   MAMATASFPPPSATAFSATSSIVFEIGKFNAVLEKVESAGCCSSGRHLPAPPPKPLLIGR 60

Query: 61  PSDPGEFPVLLLIHGYLLYNTFYSQLIQQIASHGFIVIAPQLYTLAGPDTSEEIKATAAI 120
           PS  GEFPVLLLIHGYLLYNTFYSQLI QIASHGFIVIAPQLY++AGPDTSEEIKATAA+
Sbjct: 61  PSIAGEFPVLLLIHGYLLYNTFYSQLIHQIASHGFIVIAPQLYSVAGPDTSEEIKATAAV 120

Query: 121 INWLSEGLRQLLPPHVNPNLNKIALAGHSRGGKTSFALAQHKSIPKSPKFSALIGLDPVD 180
           INWL EGLRQ LPPHVNPNLNKIAL+GHSRGGKTSFALA   +  KSPK SALIGLDPVD
Sbjct: 121 INWLPEGLRQHLPPHVNPNLNKIALSGHSRGGKTSFALALALAEQKSPKLSALIGLDPVD 180

Query: 181 GMGSGKQTHPPVLKYIPQSFDFELPVLVIGSGLGEFKRNPLFPPCAPKGINHEDFFNECR 240
           G GSGKQTHPPVLKYIPQSFD  LPVLVIGSGLGE KRNPLFPPCAPKGINHE+FF ECR
Sbjct: 181 GTGSGKQTHPPVLKYIPQSFDLGLPVLVIGSGLGELKRNPLFPPCAPKGINHEEFFKECR 240

Query: 241 SPAHYFVVKDYGHLDLLDDETGGIRGKASYCLCKNGESREPMRRFVGGAVVAFLKAYLDG 300
           +PAHYFV KDYGHLDLLDDETGG+RGKASYCLCKNG+SREPMRRFVGGAVVAFLKAYL+G
Sbjct: 241 NPAHYFVAKDYGHLDLLDDETGGLRGKASYCLCKNGDSREPMRRFVGGAVVAFLKAYLNG 300

Query: 301 EDEDLSGIVDGDLSLPVQLQTVESLL 326
           ED DL  I DGDL LPV LQTVESLL
Sbjct: 301 EDGDLRAIEDGDLCLPVHLQTVESLL 326

BLAST of Lcy01g002640 vs. TAIR 10
Match: AT5G43860.1 (chlorophyllase 2 )

HSP 1 Score: 391.3 bits (1004), Expect = 7.3e-109
Identity = 190/317 (59.94%), Postives = 238/317 (75.08%), Query Frame = 0

Query: 17  SATSPNVFEIGKFNAVLEKVDSG-----FCSSGRYLPVPPPKPLLIGRPSDPGEFPVLLL 76
           S++S N FE GK+ + L  +DS         S R  P  PPK LL+  P + G++PV++L
Sbjct: 3   SSSSRNAFEDGKYKSNLLTLDSSSRCCKITPSSRASP-SPPKQLLVATPVEEGDYPVVML 62

Query: 77  IHGYLLYNTFYSQLIQQIASHGFIVIAPQLYTLAGPDTSEEIKATAAIINWLSEGLRQLL 136
           +HGYLLYN+FYSQL+  ++SHGFI+IAPQLY++AGPDT +EIK+TA I++WLS GL   L
Sbjct: 63  LHGYLLYNSFYSQLMLHVSSHGFILIAPQLYSIAGPDTMDEIKSTAEIMDWLSVGLNHFL 122

Query: 137 PPHVNPNLNKIALAGHSRGGKTSFALAQHK-SIPKSPKFSALIGLDPVDGMGSGKQTHPP 196
           P  V PNL+K AL+GHSRGGKT+FA+A  K     + K S LIG+DPVDG G GKQT PP
Sbjct: 123 PAQVTPNLSKFALSGHSRGGKTAFAVALKKFGYSSNLKISTLIGIDPVDGTGKGKQTPPP 182

Query: 197 VLKYIPQSFDFE-LPVLVIGSGLGEFKRNPLFPPCAPKGINHEDFFNECRSPAHYFVVKD 256
           VL Y+P SFD +  P+LVIGSGLGE  RNPLFPPCAP G+NH +FF EC+ PA +FV KD
Sbjct: 183 VLAYLPNSFDLDKTPILVIGSGLGETARNPLFPPCAPPGVNHREFFRECQGPAWHFVAKD 242

Query: 257 YGHLDLLDDETGGIRGKASYCLCKNGESREPMRRFVGGAVVAFLKAYLDGEDEDLSGIVD 316
           YGHLD+LDD+T GIRGK+SYCLCKNGE R PMRRFVGG VV+FLKAYL+G+D +L  I D
Sbjct: 243 YGHLDMLDDDTKGIRGKSSYCLCKNGEERRPMRRFVGGLVVSFLKAYLEGDDRELVKIKD 302

Query: 317 G-DLSLPVQLQTVESLL 326
           G    +PV++Q  E ++
Sbjct: 303 GCHEDVPVEIQEFEVIM 318

BLAST of Lcy01g002640 vs. TAIR 10
Match: AT1G19670.1 (chlorophyllase 1 )

HSP 1 Score: 258.8 bits (660), Expect = 5.7e-69
Identity = 138/310 (44.52%), Postives = 183/310 (59.03%), Query Frame = 0

Query: 12  SATAGSATSPNVFEIGKFNAVLEKVDSGFCSSGRYLPVPPPKPLLIGRPSDPGEFPVLLL 71
           S T  S  +P  FEIG        VD     S       PPKP+ I  P+  G +PV+L 
Sbjct: 7   SPTFSSVVTPAAFEIGSLPTTEIPVDPVENDS-----TAPPKPVRITCPTVAGTYPVVLF 66

Query: 72  IHGYLLYNTFYSQLIQQIASHGFIVIAPQLYTLAGPDTSEEIKATAAIINWLSEGLRQLL 131
            HG+ L N FYS ++  IASHG+I++APQL  L  P    E+    ++INW SE L+  L
Sbjct: 67  FHGFYLRNYFYSDVLNHIASHGYILVAPQLCKLLPPGGQVEVDDAGSVINWASENLKAHL 126

Query: 132 PPHVNPNLNKIALAGHSRGGKTSFALAQHKSIPKSPK--FSALIGLDPVDGMGSGKQTHP 191
           P  VN N    +L GHSRGGKT+FA+A   +    P   FSALIG+DPV G     +T P
Sbjct: 127 PTSVNANGKYTSLVGHSRGGKTAFAVALGHAATLDPSITFSALIGIDPVAGTNKYIRTDP 186

Query: 192 PVLKYIPQSFDFELPVLVIGSGLGEFKRNPLFPPCAPKGINHEDFFNECRSPAHYFVVKD 251
            +L Y P+SF+ ++PV V+G+GLG  K N + PPCAP  +NHE+F+ EC++   +FV  D
Sbjct: 187 HILTYKPESFELDIPVAVVGTGLGP-KWNNVMPPCAPTDLNHEEFYKECKATKAHFVAAD 246

Query: 252 YGHLDLLDDETGGIRGKASYCLCKNGE-SREPMRRFVGGAVVAFLKAYLDGEDEDLSGIV 311
           YGH+D+LDD+  G  G  + C+CKNG+  +  MR FVGG VVAFLK  L GE  ++  IV
Sbjct: 247 YGHMDMLDDDLPGFVGFMAGCMCKNGQRKKSEMRSFVGGIVVAFLKYSLWGEKAEIRLIV 306

Query: 312 DGDLSLPVQL 319
                 P +L
Sbjct: 307 KDPSVSPAKL 310

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M7I71.0e-10759.94Chlorophyllase-2 OS=Arabidopsis thaliana OX=3702 GN=CLH2 PE=1 SV=1[more]
O225278.0e-6844.52Chlorophyllase-1 OS=Arabidopsis thaliana OX=3702 GN=CLH1 PE=1 SV=1[more]
Q94LX14.1e-6446.57Chlorophyllase-1, chloroplastic OS=Citrus unshiu OX=55188 PE=2 SV=1[more]
Q9MV141.0e-6246.21Chlorophyllase-1, chloroplastic OS=Citrus sinensis OX=2711 GN=CHLASE1 PE=1 SV=1[more]
Q9LE894.9e-4940.00Chlorophyllase type 0 OS=Chenopodium album OX=3559 GN=CACLH PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1H5Z82.2e-16187.12Chlorophyllase OS=Cucurbita moschata OX=3662 GN=LOC111459924 PE=3 SV=1[more]
A0A6J1KYL85.4e-16086.81Chlorophyllase OS=Cucurbita maxima OX=3661 GN=LOC111499382 PE=3 SV=1[more]
A0A6J1C9F91.2e-15181.76Chlorophyllase OS=Momordica charantia OX=3673 GN=LOC111009609 PE=3 SV=1[more]
A0A5A7UQQ12.5e-14981.60Chlorophyllase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G00131... [more]
A0A1S3BME12.5e-14981.60Chlorophyllase OS=Cucumis melo OX=3656 GN=LOC103491233 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KAG7013529.13.5e-16187.12Chlorophyllase-2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022958739.14.5e-16187.12chlorophyllase-2, chloroplastic [Cucurbita moschata][more]
KAG6574961.11.0e-16086.81Chlorophyllase-2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023548652.13.8e-16086.81chlorophyllase-2, chloroplastic [Cucurbita pepo subsp. pepo][more]
XP_023006741.11.1e-15986.81chlorophyllase-2, chloroplastic [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G43860.17.3e-10959.94chlorophyllase 2 [more]
AT1G19670.15.7e-6944.52chlorophyllase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017395ChlorophyllasePFAMPF07224Chlorophyllasecoord: 17..319
e-value: 7.4E-120
score: 399.6
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 24..308
e-value: 5.1E-29
score: 103.4
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 57..300
NoneNo IPR availablePANTHERPTHR33428:SF13CHLOROPHYLLASE-2, CHLOROPLASTICcoord: 15..322
NoneNo IPR availablePANTHERPTHR33428CHLOROPHYLLASE-2, CHLOROPLASTICcoord: 15..322

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy01g002640.1Lcy01g002640.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015996 chlorophyll catabolic process
molecular_function GO:0047746 chlorophyllase activity
molecular_function GO:0102293 pheophytinase b activity