Lcy01g002270 (gene) Sponge gourd (P93075) v1

Overview
NameLcy01g002270
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionLipoxygenase
LocationChr01: 2640495 .. 2644665 (+)
RNA-Seq ExpressionLcy01g002270
SyntenyLcy01g002270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAACCTTCAGATCAATCCAGTTTCAACAAATCTTCCTTCCTTTCTCTGCAATTTTTTTCTTTCCTTCATCTTCTTGAAGAAGACAGAGTTCTGATAGACTGCCATTTTTGCACTCTGTTCCCTTCCTTTTGGTCAAAAATGGGGATCTGTCCTGGTGTGGAGATGCTTCAGAAGATGTTGGAGGCCTTTTTCTCCGGCAATCTGATGAAGTTTGGCTACGGCGGTGGCGGCGGCGGCGGCGGAAGCAGAATTAGAGGCACCGTGGTTCTTATGAAGAAGTGCGTTCTCGACTTCAAAGACGTCAAGGCTTCGGTTCTCGATCGAGTTCATGAATTCCTCGGCAAAGGCGTCTCCATTCAACTTATCAGCTCCGATCCTTCTAGTTCAGGTCCATCCGATTCTAGCTTTTGAATTCAGTGGTGATTTAAGGTAGTATCAGAGTCAATAATTTCTTTGTTGCTGGTACTGGTACTGCAACTCTGATATGAAAACTAATATTTTTTTTAACATGGTATCGGAGCCGATATAATTTTCTACTTTGTTGATTGAACTTGTCAATTCAGTTTTTCTTTTTACTGTAACTTGGTTCTAAAGGTATTTTACTTGTTTCAATTACTACGGTCATTTAAACTTTTGAGTTCAGTGGTAATTTTTGTAGTTGTTGTTACTATAATTTGTTGGTTTAAACTTTCGAATGGTCATCGGGTGCGTGCAGGAGATAGATTGGGGGCGGCGAAGGTGGGGAAGGCGGCGAATTTGGAGCAGTGGATATTGACGATGAAGCCGTCGTTAACTGCGGACGACATCGAGTTCACAGTGTTCTTTGAATGGGAGGAGAGGATGGGAGTACCAGGAGCTTTCATCATCAGAAACCATCACTCGAGCCAGTTTTTCTTGAAGACAGTCACCTTGCACGATGTTCCTGGCCATGGCTCTGTCCATTTCGTCTGCAACTCTTGGGTTTATCCTGCTCATCGTTATACTTACGATCGTGTCTTTTTCTCAAACAAGGTAAAATCTTATGAGAGGTCTGTTTGGAATGACCTTTTAAGTATTTAAAAACACTTTTTTAAATACTTACAAAGTCCAAATGGACTCATATTCTTCTTTGTTTGAAGAAAATTTTATATCGAAAAGATTAAAATTCTTTCGTTAGTATATAGAAATAAGATTATGGTTTCGAGAAAGATTATTAATGCCTTGGTGACTAAGTTAGATGAGTAAACTTTTTCGTTTTAGATATTGTAAATTTTGATTAGTTTTTGTTCTTGATTCCATTATTGTCGGTGGCTGGCTATCTTTATATCTTTTTGCTGTTACCTTCCATGTGAAGTCAATTAATCATTTCTGCAAAATAATCAATGAGGTTAAGGTATTATATACCTTTTCTAAGGTATAAAGATTTGAATCTTTAAATTCAGAAAAGAAAAAAAGAACAAAAAAGGGCTGAAGCATAACAAATCTGTCAACTCTTCAATACAGTTAAAAAAAAAAAAAGGATATACATTGATGTTGATGCCTTTGATTTTCCCTTTTGCAATTTTTTTTAGTAACAGGAAATGATTTTGTTTGATCGTTTTGTTTGTTAGAGTTACCTTCCGAGTGAAACACCAGAGCCACTTCGAAAGTATAGAGAAGAAGAACTTGTTAACCTCCGAGGAAATGGCATTGGTGAGCTCAAGGAGTGGGATAGAGTTTATGACTATGCTTACTACAACGATCTCGGAATGCCAGACATGGGCAAAAGGTTTGTTCGTACTGTTCTTGGTGGTTCGAAGGAGTTTCCCTATCCTCGAAGAGGACGAACCGGTCGAAAACCAGCAAAAGCAGGTTGAGAATATGATCTTTGAATTAATTCTTTCACGTTCATCTTCATTATGGTTTGATTCTGATTTTCGACCTGATTATGTTGTTACAGATCCATGCTGTGAAAGCAGGTTGCCATTGCTGAGTTTAGACATTTACGTGCCGAGAGACGAGCGGTTTCGGAACCTGAAATTTTCGGATTTTTTAGCTTATGCAGTGAAGTCAATTTTCCAGACATTGGTTCCAGAGATCAAATCTTTATGTGATAAAACTATCAATGAGTTTGACTGCTTTCAAGATGTTCTGGATCTTTATGAAGGAGGAATGAGCCTACCAAATGAAATACTAGGCAGCCTAAGGGAACTGGTTCCTTGGCAGCTGTTTAGGGAGCTGATGCGTTCCGATGGTCGCCAATTCCTGAAATTTCCGGTGCCCGACGTGATCAAAGGTACACGAATATATAATCTACGACGTCGAGCTTTCGAAAGAACTCTACTAGAATGCTAAATACTACTTGGCATTGCAATGGCAGAGAGCAAAACAGCTTGGAGGACAGACGAAGAGTTCGGACGAGAAATGCTTGCTGGAGTTAACCCGGTCATCATTCGTCGACTTGAAGTACGACACACTTTGAAATCCATAGCTTCATTTACTAAATTCCCTAGGGTCCCTTTTCATCCATAATTCACTTTAAAATGTTTCTCTGTAGGAGTTCCCTCCAGCCAGCAAGTTGGATCCTAAAGCATATGGAAAGCAGAACAGTTCCATAACAGAAGGGCACATAGCACAGCACTTGAATGGGCTCACTGTTGATCAGGCATTGGAAATGAACAAGCTGTTCATTCTGGATCATCATGATTCACTGATGCCATACATTAGTAGAATAAACTCGACGTCGACGAAGATGTACGCTACCCGAACAGTCCTCCTTCTGCAGGACAATGGCATCTTGAAGCCATTGGCAATTGAACTAAGTCTGCCACACCCACAAGGGGATCACCATGGAGCTGTGAGCAAAGTTTTCACTCCAGCAGAACACGGCGTCGAGGGATCCGTTTGGCAGTTGGCTAAAGCTTACGTCGCTGTGAGCGATTCCGGTTATCACCAACTGATTAGCCATTGGTAAAATCCACAACACTAGAACAATCAATAAACAGGATTGAATCATCTTCCTCAACTTAAATCTAAACAGCTCAAATTCACTGCACTTGCAGGCTGAACACTCATGCTGTGATAGAGCCATTCGTTATAGCAACAAACAGACAATTAAGTGTCATGCATCCAATCTATAAGCTTCTTCACCCACACTTCAGGGACACAATGAACATAAACGCCATGGCTAGGCAGGTTCTCATCAACGCTGGTGGAATTCTAGAAACCACTGTCTTCCCAGGAAAATACGCCTTGGAAATGTCAGCTGTTCTTTACAAGAACTGGATCTTCACAGACCAGGCACTCCCAGCCGATCTAATCAAGAGGTGGGTTCTGGGTTGTGTTCATTTCAGTCAAACTCAAAATCAATTTCCCCTGTTCCTAAATTTCATAAACATGAATCCATGACAATGAAATCAAAATTTTCTTACCCCATTTTACAGAGGAGTGGCAGTTCCCGATCCAGACAGCCCCCATGGCCTCAAGCTTCTGATAGAGGATTATCCTTATGCAGTGGATGGGCTCGAGATCTGGTCCGCAATCGAGACATGGGTTAGAGAATACTGTTGTTTCTACTACAAATCAGACGAAATGGTTGAAGAAGACACCGAAATTCAATCGTGGTGGACAGAGCTTCGCAATGTCGGGCATGGCGATCTGAAAGACGAACCATGGTGGCCGAAAATGAAGACGAGAGAAGAATTAATCCAATCGTGCACCATCGTGATTTGGATAGCTTCGGCCCTCCACGCCGCCGTGAACTTCGGGCAATACCCATACGCCGGGTACCTACCAAACCGGCCGACGGTGAGCCGGCGGTTCATGCCGGAACCAGGGACGCCGGAGTTCAGAGAGCTGGAGACGGACCCGGATTTAGCCTACTTGAAGACGATTACAGCGCAGCTACAGACGATCCTGGGCGTGTCGCTGATCGAGAGCTTATCTCGACATTCGGTGGACGAGATTTATCTGGGGCAGAGAGAAACGGCGGATTGGACGAGCGATGAAGAAGCAGTGGCGGCATTCGAGAGATTTGGGGATCGATTGAAGGAGATTGAGGAGAAGATTATGAGGATGAACAACGATGAGAGATGGAGGAATCGAGTTGGGCCGGTGAAAATGCCGTATACATTGCTGTTTCCGAATACCTCGAATTACTATGAAGAAGAAGGTCTGAATGCCAGAGGAATCCCAAATAGCATTTCGATCTGA

mRNA sequence

AAAACCTTCAGATCAATCCAGTTTCAACAAATCTTCCTTCCTTTCTCTGCAATTTTTTTCTTTCCTTCATCTTCTTGAAGAAGACAGAGTTCTGATAGACTGCCATTTTTGCACTCTGTTCCCTTCCTTTTGGTCAAAAATGGGGATCTGTCCTGGTGTGGAGATGCTTCAGAAGATGTTGGAGGCCTTTTTCTCCGGCAATCTGATGAAGTTTGGCTACGGCGGTGGCGGCGGCGGCGGCGGAAGCAGAATTAGAGGCACCGTGGTTCTTATGAAGAAGTGCGTTCTCGACTTCAAAGACGTCAAGGCTTCGGTTCTCGATCGAGTTCATGAATTCCTCGGCAAAGGCGTCTCCATTCAACTTATCAGCTCCGATCCTTCTAGTTCAGGAGATAGATTGGGGGCGGCGAAGGTGGGGAAGGCGGCGAATTTGGAGCAGTGGATATTGACGATGAAGCCGTCGTTAACTGCGGACGACATCGAGTTCACAGTGTTCTTTGAATGGGAGGAGAGGATGGGAGTACCAGGAGCTTTCATCATCAGAAACCATCACTCGAGCCAGTTTTTCTTGAAGACAGTCACCTTGCACGATGTTCCTGGCCATGGCTCTGTCCATTTCGTCTGCAACTCTTGGGTTTATCCTGCTCATCGTTATACTTACGATCGTGTCTTTTTCTCAAACAAGAGTTACCTTCCGAGTGAAACACCAGAGCCACTTCGAAAGTATAGAGAAGAAGAACTTGTTAACCTCCGAGGAAATGGCATTGGTGAGCTCAAGGAGTGGGATAGAGTTTATGACTATGCTTACTACAACGATCTCGGAATGCCAGACATGGGCAAAAGGTTTGTTCGTACTGTTCTTGGTGGTTCGAAGGAGTTTCCCTATCCTCGAAGAGGACGAACCGGTCGAAAACCAGCAAAAGCAGATCCATGCTGTGAAAGCAGGTTGCCATTGCTGAGTTTAGACATTTACGTGCCGAGAGACGAGCGGTTTCGGAACCTGAAATTTTCGGATTTTTTAGCTTATGCAGTGAAGTCAATTTTCCAGACATTGGTTCCAGAGATCAAATCTTTATGTGATAAAACTATCAATGAGTTTGACTGCTTTCAAGATGTTCTGGATCTTTATGAAGGAGGAATGAGCCTACCAAATGAAATACTAGGCAGCCTAAGGGAACTGGTTCCTTGGCAGCTGTTTAGGGAGCTGATGCGTTCCGATGGTCGCCAATTCCTGAAATTTCCGGTGCCCGACGTGATCAAAGAGAGCAAAACAGCTTGGAGGACAGACGAAGAGTTCGGACGAGAAATGCTTGCTGGAGTTAACCCGGTCATCATTCGTCGACTTGAAGAGTTCCCTCCAGCCAGCAAGTTGGATCCTAAAGCATATGGAAAGCAGAACAGTTCCATAACAGAAGGGCACATAGCACAGCACTTGAATGGGCTCACTGTTGATCAGGCATTGGAAATGAACAAGCTGTTCATTCTGGATCATCATGATTCACTGATGCCATACATTAGTAGAATAAACTCGACGTCGACGAAGATGTACGCTACCCGAACAGTCCTCCTTCTGCAGGACAATGGCATCTTGAAGCCATTGGCAATTGAACTAAGTCTGCCACACCCACAAGGGGATCACCATGGAGCTGTGAGCAAAGTTTTCACTCCAGCAGAACACGGCGTCGAGGGATCCGTTTGGCAGTTGGCTAAAGCTTACGTCGCTGTGAGCGATTCCGGTTATCACCAACTGATTAGCCATTGGCTGAACACTCATGCTGTGATAGAGCCATTCGTTATAGCAACAAACAGACAATTAAGTGTCATGCATCCAATCTATAAGCTTCTTCACCCACACTTCAGGGACACAATGAACATAAACGCCATGGCTAGGCAGGTTCTCATCAACGCTGGTGGAATTCTAGAAACCACTGTCTTCCCAGGAAAATACGCCTTGGAAATGTCAGCTGTTCTTTACAAGAACTGGATCTTCACAGACCAGGCACTCCCAGCCGATCTAATCAAGAGAGGAGTGGCAGTTCCCGATCCAGACAGCCCCCATGGCCTCAAGCTTCTGATAGAGGATTATCCTTATGCAGTGGATGGGCTCGAGATCTGGTCCGCAATCGAGACATGGGTTAGAGAATACTGTTGTTTCTACTACAAATCAGACGAAATGGTTGAAGAAGACACCGAAATTCAATCGTGGTGGACAGAGCTTCGCAATGTCGGGCATGGCGATCTGAAAGACGAACCATGGTGGCCGAAAATGAAGACGAGAGAAGAATTAATCCAATCGTGCACCATCGTGATTTGGATAGCTTCGGCCCTCCACGCCGCCGTGAACTTCGGGCAATACCCATACGCCGGGTACCTACCAAACCGGCCGACGGTGAGCCGGCGGTTCATGCCGGAACCAGGGACGCCGGAGTTCAGAGAGCTGGAGACGGACCCGGATTTAGCCTACTTGAAGACGATTACAGCGCAGCTACAGACGATCCTGGGCGTGTCGCTGATCGAGAGCTTATCTCGACATTCGGTGGACGAGATTTATCTGGGGCAGAGAGAAACGGCGGATTGGACGAGCGATGAAGAAGCAGTGGCGGCATTCGAGAGATTTGGGGATCGATTGAAGGAGATTGAGGAGAAGATTATGAGGATGAACAACGATGAGAGATGGAGGAATCGAGTTGGGCCGGTGAAAATGCCGTATACATTGCTGTTTCCGAATACCTCGAATTACTATGAAGAAGAAGGTCTGAATGCCAGAGGAATCCCAAATAGCATTTCGATCTGA

Coding sequence (CDS)

ATGGGGATCTGTCCTGGTGTGGAGATGCTTCAGAAGATGTTGGAGGCCTTTTTCTCCGGCAATCTGATGAAGTTTGGCTACGGCGGTGGCGGCGGCGGCGGCGGAAGCAGAATTAGAGGCACCGTGGTTCTTATGAAGAAGTGCGTTCTCGACTTCAAAGACGTCAAGGCTTCGGTTCTCGATCGAGTTCATGAATTCCTCGGCAAAGGCGTCTCCATTCAACTTATCAGCTCCGATCCTTCTAGTTCAGGAGATAGATTGGGGGCGGCGAAGGTGGGGAAGGCGGCGAATTTGGAGCAGTGGATATTGACGATGAAGCCGTCGTTAACTGCGGACGACATCGAGTTCACAGTGTTCTTTGAATGGGAGGAGAGGATGGGAGTACCAGGAGCTTTCATCATCAGAAACCATCACTCGAGCCAGTTTTTCTTGAAGACAGTCACCTTGCACGATGTTCCTGGCCATGGCTCTGTCCATTTCGTCTGCAACTCTTGGGTTTATCCTGCTCATCGTTATACTTACGATCGTGTCTTTTTCTCAAACAAGAGTTACCTTCCGAGTGAAACACCAGAGCCACTTCGAAAGTATAGAGAAGAAGAACTTGTTAACCTCCGAGGAAATGGCATTGGTGAGCTCAAGGAGTGGGATAGAGTTTATGACTATGCTTACTACAACGATCTCGGAATGCCAGACATGGGCAAAAGGTTTGTTCGTACTGTTCTTGGTGGTTCGAAGGAGTTTCCCTATCCTCGAAGAGGACGAACCGGTCGAAAACCAGCAAAAGCAGATCCATGCTGTGAAAGCAGGTTGCCATTGCTGAGTTTAGACATTTACGTGCCGAGAGACGAGCGGTTTCGGAACCTGAAATTTTCGGATTTTTTAGCTTATGCAGTGAAGTCAATTTTCCAGACATTGGTTCCAGAGATCAAATCTTTATGTGATAAAACTATCAATGAGTTTGACTGCTTTCAAGATGTTCTGGATCTTTATGAAGGAGGAATGAGCCTACCAAATGAAATACTAGGCAGCCTAAGGGAACTGGTTCCTTGGCAGCTGTTTAGGGAGCTGATGCGTTCCGATGGTCGCCAATTCCTGAAATTTCCGGTGCCCGACGTGATCAAAGAGAGCAAAACAGCTTGGAGGACAGACGAAGAGTTCGGACGAGAAATGCTTGCTGGAGTTAACCCGGTCATCATTCGTCGACTTGAAGAGTTCCCTCCAGCCAGCAAGTTGGATCCTAAAGCATATGGAAAGCAGAACAGTTCCATAACAGAAGGGCACATAGCACAGCACTTGAATGGGCTCACTGTTGATCAGGCATTGGAAATGAACAAGCTGTTCATTCTGGATCATCATGATTCACTGATGCCATACATTAGTAGAATAAACTCGACGTCGACGAAGATGTACGCTACCCGAACAGTCCTCCTTCTGCAGGACAATGGCATCTTGAAGCCATTGGCAATTGAACTAAGTCTGCCACACCCACAAGGGGATCACCATGGAGCTGTGAGCAAAGTTTTCACTCCAGCAGAACACGGCGTCGAGGGATCCGTTTGGCAGTTGGCTAAAGCTTACGTCGCTGTGAGCGATTCCGGTTATCACCAACTGATTAGCCATTGGCTGAACACTCATGCTGTGATAGAGCCATTCGTTATAGCAACAAACAGACAATTAAGTGTCATGCATCCAATCTATAAGCTTCTTCACCCACACTTCAGGGACACAATGAACATAAACGCCATGGCTAGGCAGGTTCTCATCAACGCTGGTGGAATTCTAGAAACCACTGTCTTCCCAGGAAAATACGCCTTGGAAATGTCAGCTGTTCTTTACAAGAACTGGATCTTCACAGACCAGGCACTCCCAGCCGATCTAATCAAGAGAGGAGTGGCAGTTCCCGATCCAGACAGCCCCCATGGCCTCAAGCTTCTGATAGAGGATTATCCTTATGCAGTGGATGGGCTCGAGATCTGGTCCGCAATCGAGACATGGGTTAGAGAATACTGTTGTTTCTACTACAAATCAGACGAAATGGTTGAAGAAGACACCGAAATTCAATCGTGGTGGACAGAGCTTCGCAATGTCGGGCATGGCGATCTGAAAGACGAACCATGGTGGCCGAAAATGAAGACGAGAGAAGAATTAATCCAATCGTGCACCATCGTGATTTGGATAGCTTCGGCCCTCCACGCCGCCGTGAACTTCGGGCAATACCCATACGCCGGGTACCTACCAAACCGGCCGACGGTGAGCCGGCGGTTCATGCCGGAACCAGGGACGCCGGAGTTCAGAGAGCTGGAGACGGACCCGGATTTAGCCTACTTGAAGACGATTACAGCGCAGCTACAGACGATCCTGGGCGTGTCGCTGATCGAGAGCTTATCTCGACATTCGGTGGACGAGATTTATCTGGGGCAGAGAGAAACGGCGGATTGGACGAGCGATGAAGAAGCAGTGGCGGCATTCGAGAGATTTGGGGATCGATTGAAGGAGATTGAGGAGAAGATTATGAGGATGAACAACGATGAGAGATGGAGGAATCGAGTTGGGCCGGTGAAAATGCCGTATACATTGCTGTTTCCGAATACCTCGAATTACTATGAAGAAGAAGGTCTGAATGCCAGAGGAATCCCAAATAGCATTTCGATCTGA

Protein sequence

MGICPGVEMLQKMLEAFFSGNLMKFGYGGGGGGGGSRIRGTVVLMKKCVLDFKDVKASVLDRVHEFLGKGVSIQLISSDPSSSGDRLGAAKVGKAANLEQWILTMKPSLTADDIEFTVFFEWEERMGVPGAFIIRNHHSSQFFLKTVTLHDVPGHGSVHFVCNSWVYPAHRYTYDRVFFSNKSYLPSETPEPLRKYREEELVNLRGNGIGELKEWDRVYDYAYYNDLGMPDMGKRFVRTVLGGSKEFPYPRRGRTGRKPAKADPCCESRLPLLSLDIYVPRDERFRNLKFSDFLAYAVKSIFQTLVPEIKSLCDKTINEFDCFQDVLDLYEGGMSLPNEILGSLRELVPWQLFRELMRSDGRQFLKFPVPDVIKESKTAWRTDEEFGREMLAGVNPVIIRRLEEFPPASKLDPKAYGKQNSSITEGHIAQHLNGLTVDQALEMNKLFILDHHDSLMPYISRINSTSTKMYATRTVLLLQDNGILKPLAIELSLPHPQGDHHGAVSKVFTPAEHGVEGSVWQLAKAYVAVSDSGYHQLISHWLNTHAVIEPFVIATNRQLSVMHPIYKLLHPHFRDTMNINAMARQVLINAGGILETTVFPGKYALEMSAVLYKNWIFTDQALPADLIKRGVAVPDPDSPHGLKLLIEDYPYAVDGLEIWSAIETWVREYCCFYYKSDEMVEEDTEIQSWWTELRNVGHGDLKDEPWWPKMKTREELIQSCTIVIWIASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEFRELETDPDLAYLKTITAQLQTILGVSLIESLSRHSVDEIYLGQRETADWTSDEEAVAAFERFGDRLKEIEEKIMRMNNDERWRNRVGPVKMPYTLLFPNTSNYYEEEGLNARGIPNSISI
Homology
BLAST of Lcy01g002270 vs. ExPASy Swiss-Prot
Match: Q43191 (Probable linoleate 9S-lipoxygenase 5 OS=Solanum tuberosum OX=4113 GN=LOX1.5 PE=2 SV=1)

HSP 1 Score: 1243.8 bits (3217), Expect = 0.0e+00
Identity = 591/878 (67.31%), Postives = 723/878 (82.35%), Query Frame = 0

Query: 9   MLQKMLEAFFSGNLMKFGYGGGGGGGGSRIRGTVVLMKKCVLDFKDVKASVLDRVHEFLG 68
           +L+K++EA            G     G +++GT+VLMKK VLDF DV AS+LD V EFLG
Sbjct: 2   LLEKIVEAI----------SGRSEDNGKKVKGTIVLMKKNVLDFNDVNASLLDGVLEFLG 61

Query: 69  KGVSIQLISSDPSSSGDRLGAAKVGKAANLEQWILTMKPSLTADDIEFTVFFEWEERMGV 128
           K VS+QLIS   +  G+ L   K    A LE+W LT   SL A +  F V F+W+E +GV
Sbjct: 62  KRVSLQLISVVHADPGNSL-QGKRSNPAYLEKW-LTTGTSLVAGESAFDVTFDWDEDIGV 121

Query: 129 PGAFIIRNHHSSQFFLKTVTLHDVPGHGSVHFVCNSWVYPAHRYTYDRVFFSNKSYLPSE 188
           PGAFII N H ++F+LK++TL DVP HG+VHFVCNSWVYPA +Y  +R+FF+N++YLP E
Sbjct: 122 PGAFIINNFHFNEFYLKSLTLEDVPNHGNVHFVCNSWVYPAKKYKSERIFFANQAYLPGE 181

Query: 189 TPEPLRKYREEELVNLRGNGIGELKEWDRVYDYAYYNDLGMPDMGKRFVRTVLGGSKEFP 248
           TPEPLR YRE+ELVNLRGNG G+L+EWDRVYDYA YNDLG P+ GK++ RT+LGGS E+P
Sbjct: 182 TPEPLRNYREKELVNLRGNGNGKLEEWDRVYDYALYNDLGDPEKGKQYARTILGGSAEYP 241

Query: 249 YPRRGRTGRKPAKADPCCESRLPLL-SLDIYVPRDERFRNLKFSDFLAYAVKSIFQTLVP 308
           YPRRGRTGRKP KADP  ESR+PLL SLDIYVPRDERF ++K SDFL YA+KSI Q L+P
Sbjct: 242 YPRRGRTGRKPTKADPKSESRIPLLMSLDIYVPRDERFGHIKLSDFLTYALKSIVQFLIP 301

Query: 309 EIKSLCDKTINEFDCFQDVLDLYEGGMSLP-NEILGSLRELVPWQLFRELMRSDGRQFLK 368
           E ++L D T +EFD F+DVL LYEGG+ LP    L +L + +P ++ +E++R+DG    K
Sbjct: 302 EFQALFDSTPDEFDSFEDVLKLYEGGIKLPQGPFLKALTDSIPLEILKEIIRTDGEGKFK 361

Query: 369 FPVPDVIKESKTAWRTDEEFGREMLAGVNPVIIRRLEEFPPASKLDPKAYGKQNSSITEG 428
           FP P VI+E K++WRTDEEF REMLAGVNPVII RL+EFPP S+LD + YG QNS+IT+ 
Sbjct: 362 FPTPQVIQEDKSSWRTDEEFAREMLAGVNPVIISRLQEFPPKSQLDSEVYGNQNSTITKE 421

Query: 429 HIAQHLNGLTVDQALEMNKLFILDHHDSLMPYISRINSTSTKMYATRTVLLLQDNGILKP 488
           HI   L+GLT+D A++ N+L+IL+HHD LMPY+ RIN+T+TK+YA+RT+L LQD+G +KP
Sbjct: 422 HIENTLDGLTIDDAIKTNRLYILNHHDILMPYVRRINTTNTKLYASRTLLFLQDDGTMKP 481

Query: 489 LAIELSLPHPQGDHHGAVSKVFTPAEHGVEGSVWQLAKAYVAVSDSGYHQLISHWLNTHA 548
           +AIELSLPHP GD  GAVSKV+TPA+ GVEGS+WQLAKAYVAV+DSG HQLISHWLNTHA
Sbjct: 482 VAIELSLPHPDGDELGAVSKVYTPADQGVEGSIWQLAKAYVAVNDSGVHQLISHWLNTHA 541

Query: 549 VIEPFVIATNRQLSVMHPIYKLLHPHFRDTMNINAMARQVLINAGGILETTVFPGKYALE 608
            IEPFVIATNRQLSV+HPI+KLLHPHFRDTMNINA+ARQ+LINAGG+LE TVFP KYA+E
Sbjct: 542 AIEPFVIATNRQLSVLHPIHKLLHPHFRDTMNINALARQILINAGGVLEMTVFPAKYAME 601

Query: 609 MSAVLYKNWIFTDQALPADLIKRGVAVPDPDSPHGLKLLIEDYPYAVDGLEIWSAIETWV 668
           MSAV+YK+W+F +QALPADLIKRGVAV D  SPHG++LLI+DYPYAVDGLEIWSAI++WV
Sbjct: 602 MSAVVYKSWVFPEQALPADLIKRGVAVEDSSSPHGVRLLIQDYPYAVDGLEIWSAIKSWV 661

Query: 669 REYCCFYYKSDEMVEEDTEIQSWWTELRNVGHGDLKDEPWWPKMKTREELIQSCTIVIWI 728
            EYC FYYKSDE+V +D E+Q+WW ELR  GHGD KDEPWWPKM+TR+EL  SCTI+IWI
Sbjct: 662 TEYCNFYYKSDELVLKDNELQAWWKELREEGHGDKKDEPWWPKMQTRQELKDSCTIIIWI 721

Query: 729 ASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEFRELETDPDLAYLKTITAQLQTI 788
           ASALHAAVNFGQYPYAGYLPNRPT+SRRFMPEPGTPE+ EL+T+PD AYLKTIT QLQT+
Sbjct: 722 ASALHAAVNFGQYPYAGYLPNRPTLSRRFMPEPGTPEYEELKTNPDKAYLKTITPQLQTL 781

Query: 789 LGVSLIESLSRHSVDEIYLGQRETADWTSDEEAVAAFERFGDRLKEIEEKIMRMNNDERW 848
           LG+SLIE LSRH+ DEIYLGQR++++WT D+E +AAFERFG +L EIE++I++MN D++W
Sbjct: 782 LGISLIEILSRHASDEIYLGQRDSSEWTKDQEPIAAFERFGKKLSEIEDQIIQMNGDKKW 841

Query: 849 RNRVGPVKMPYTLLFPNTSNYYEEEGLNARGIPNSISI 885
           +NR GPV +PYTLLFP +     E+GL  +GIPNS+SI
Sbjct: 842 KNRSGPVNVPYTLLFPTS-----EQGLTGKGIPNSVSI 862

BLAST of Lcy01g002270 vs. ExPASy Swiss-Prot
Match: Q9LUW0 (Linoleate 9S-lipoxygenase 5 OS=Arabidopsis thaliana OX=3702 GN=LOX5 PE=1 SV=2)

HSP 1 Score: 1236.9 bits (3199), Expect = 0.0e+00
Identity = 580/855 (67.84%), Postives = 714/855 (83.51%), Query Frame = 0

Query: 37  RIRGTVVLMKKCVLDFKDVKASVLDRVHEFLGKGVSIQLISS-DPSSSGDRLGAAKVGKA 96
           +I G VV+MKK +LDFKDV AS+LDRV+E LG+ VS+ LISS  P  + ++ G  ++GKA
Sbjct: 34  KIEGEVVVMKKNLLDFKDVMASLLDRVNELLGRRVSLHLISSHQPDPANEKRG--RLGKA 93

Query: 97  ANLEQWILTMKPSLTADDIEFTVFFEWEERMGVPGAFIIRNHHSSQFFLKTVTLHDVP-- 156
           A+LE+W+  +K S+TA++  F V F+W+E MG P AF+I+NHH SQF+LK++TL   P  
Sbjct: 94  AHLEKWVTKIKTSVTAEETAFGVTFDWDESMGPPAAFVIKNHHHSQFYLKSLTLRGFPDG 153

Query: 157 --GHGSVHFVCNSWVYPAHRYTYDRVFFSNKSYLPSETPEPLRKYREEELVNLRGNGI-G 216
             G  ++HF+CNSW+YP HRY  DRVFFSNK+YLPSETPE +++ REEEL NLRGN   G
Sbjct: 154 EGGATAIHFICNSWIYPNHRYRSDRVFFSNKAYLPSETPELIKELREEELKNLRGNEKGG 213

Query: 217 ELKEWDRVYDYAYYNDLGMPDMGKRFVRTVLGGSKEFPYPRRGRTGRKPAKADPCCESRL 276
           E KEWDRVYDYAYYNDLG PD G   VR VLGGS E PYPRRG+TGRK  K+DP  ESRL
Sbjct: 214 EFKEWDRVYDYAYYNDLGAPDKGPDSVRPVLGGSPELPYPRRGKTGRKSTKSDPKSESRL 273

Query: 277 PLLSLDIYVPRDERFRNLKFSDFLAYAVKSIFQTLVPEIKSLCDKTINEFDCFQDVLDLY 336
            LL+L+IYVPRDERF ++KFSDFLAYA+KS+ Q LVPEI S+CDKTINEFD F+DV  LY
Sbjct: 274 ALLNLNIYVPRDERFSHVKFSDFLAYALKSVTQVLVPEIASVCDKTINEFDSFEDVFHLY 333

Query: 337 EGGMSLPN-EILGSLRELVPWQLFRELMRSDGRQFLKFPVPDVIKESKTAWRTDEEFGRE 396
           +G + L N   +  LR+++PW++FREL+R+DG +FLK+P+PD++KES++AWRTDEEF RE
Sbjct: 334 DGSIKLANGHTISKLRDVIPWEMFRELVRNDGERFLKYPLPDILKESRSAWRTDEEFARE 393

Query: 397 MLAGVNPVIIRRLEEFPPASKLDPKAYGKQNSSITEGHIAQHLNGLTVDQALEMNKLFIL 456
           MLAG+NPV+I RL+EFPP S LD   YG Q+SSI   HI  ++NGL V +ALE NKL+IL
Sbjct: 394 MLAGLNPVVISRLQEFPPKSCLDSAKYGNQHSSIRTEHIESNMNGLNVQEALEQNKLYIL 453

Query: 457 DHHDSLMPYISRINSTSTKMYATRTVLLLQDNGILKPLAIELSLPHPQGDHHGAVSKVFT 516
           DHHD+LMPY++RINST+TK YATRT+LLLQ +G LKPLAIELSLPH QG+ +G+VSKVFT
Sbjct: 454 DHHDALMPYLTRINSTNTKTYATRTLLLLQADGTLKPLAIELSLPHAQGESYGSVSKVFT 513

Query: 517 PAEHGVEGSVWQLAKAYVAVSDSGYHQLISHWLNTHAVIEPFVIATNRQLSVMHPIYKLL 576
           PAE GVEGSVWQLAKAY AV+DSGYHQLISHWL THAVIEPF+IA+NRQLSV+HPI+KLL
Sbjct: 514 PAEKGVEGSVWQLAKAYAAVNDSGYHQLISHWLQTHAVIEPFIIASNRQLSVVHPIHKLL 573

Query: 577 HPHFRDTMNINAMARQVLINAGGILETTVFPGKYALEMSAVLYKNWIFTDQALPADLIKR 636
           HPHFRDTMNINA+AR VLIN+ G+LE TVFP +YA+EMS+ +YKNW+FT+QALP DL+KR
Sbjct: 574 HPHFRDTMNINALARHVLINSDGVLERTVFPSRYAMEMSSSIYKNWVFTEQALPKDLLKR 633

Query: 637 GVAVPDPDSPHGLKLLIEDYPYAVDGLEIWSAIETWVREYCCFYYKSDEMVEEDTEIQSW 696
           GVAV DP+S +G+KLLIEDYP+AVDGLEIWSAI+TWV EYC FYY +D+ V+ DTEIQSW
Sbjct: 634 GVAVEDPNSDNGVKLLIEDYPFAVDGLEIWSAIKTWVTEYCTFYYNNDKTVQTDTEIQSW 693

Query: 697 WTELRNVGHGDLKDEPWWPKMKTREELIQSCTIVIWIASALHAAVNFGQYPYAGYLPNRP 756
           WTELR  GHGD + E WWP M+TR++LI++CTI+IWIASALHAAVNFGQYPYAG+LPNRP
Sbjct: 694 WTELRTKGHGDKRHESWWPSMQTRDDLIETCTIIIWIASALHAAVNFGQYPYAGFLPNRP 753

Query: 757 TVSRRFMPEPGTPEFRELETDPDLAYLKTITAQLQTILGVSLIESLSRHSVDEIYLGQRE 816
           TVSRRFMPEPGT E+ ELE D D+A+LKTIT QLQT+LG+S+IE LS HS DEIYLGQR+
Sbjct: 754 TVSRRFMPEPGTDEYAELEEDADVAFLKTITPQLQTLLGISIIEILSMHSTDEIYLGQRD 813

Query: 817 TADWTSDEEAVAAFERFGDRLKEIEEKIMRMNNDERWRNRVGPVKMPYTLLFPNTSNYYE 876
           + +WT+D+E + AF+RFG  L+ IE  I+R NND+R++NR GPV +PYTLL+PNT++Y  
Sbjct: 814 SPNWTADDEPLEAFKRFGKELELIENNIIRRNNDKRFKNRTGPVNIPYTLLYPNTTDYTR 873

Query: 877 EEGLNARGIPNSISI 885
           E G+  +GIPNS+SI
Sbjct: 874 EGGITGKGIPNSVSI 886

BLAST of Lcy01g002270 vs. ExPASy Swiss-Prot
Match: Q43190 (Probable linoleate 9S-lipoxygenase 4 OS=Solanum tuberosum OX=4113 GN=LOX1.4 PE=2 SV=1)

HSP 1 Score: 1198.0 bits (3098), Expect = 0.0e+00
Identity = 574/857 (66.98%), Postives = 691/857 (80.63%), Query Frame = 0

Query: 30  GGGGGGSRIRGTVVLMKKCVLDFKDVKASVLDRVHEFLGKGVSIQLISSDPSSSGDRLGA 89
           GG     +++GTVV+MKK  LDF D+  S+ D++ E LG+ VS QLISS  S   + L  
Sbjct: 11  GGHHDSKKVKGTVVMMKKNALDFTDLAGSLTDKIFEALGQKVSFQLISSVQSDPANGL-Q 70

Query: 90  AKVGKAANLEQWILTMKPSLTADDIEFTVFFEWEERMGVPGAFIIRNHHSSQFFLKTVTL 149
            K    A LE ++ T+ P L A +  F V F+W E  GVPGAFII+N H ++FFLK++TL
Sbjct: 71  GKHSNPAYLENFLFTLTP-LAAGETAFGVTFDWNEEFGVPGAFIIKNTHINEFFLKSLTL 130

Query: 150 HDVPGHGSVHFVCNSWVYPAHRYTYDRVFFSNKSYLPSETPEPLRKYREEELVNLRGNGI 209
            DVP HG VHFVCNSWVYP+ RY  DR+FF+N+ YLPSETPE LRKYRE EL+ LRG+G 
Sbjct: 131 EDVPNHGKVHFVCNSWVYPSFRYKSDRIFFANQPYLPSETPELLRKYRENELLTLRGDGT 190

Query: 210 GELKEWDRVYDYAYYNDLGMPDMGKRFVRTVLGGSKEFPYPRRGRTGRKPAKADPCCESR 269
           G+ + WDR+YDY  YNDLG PD GK  VRT LGGS ++PYPRRGRTGR P + DP  ESR
Sbjct: 191 GKREAWDRIYDYDVYNDLGNPDQGKENVRTTLGGSADYPYPRRGRTGRPPTRTDPKSESR 250

Query: 270 LPL-LSLDIYVPRDERFRNLKFSDFLAYAVKSIFQTLVPEIKSLCDKTINEFDCFQDVLD 329
           +PL LSLDIYVPRDERF +LK SDFL YA+KSI Q ++PE+ +L D T NEFD F+DVL 
Sbjct: 251 IPLILSLDIYVPRDERFGHLKMSDFLTYALKSIVQFILPELHALFDGTPNEFDSFEDVLR 310

Query: 330 LYEGGMSLP-NEILGSLRELVPWQLFRELMRSDGRQFLKFPVPDVIKESKTAWRTDEEFG 389
           LYEGG+ LP   +  +L + +P ++ REL+R+DG   L+FP P VIK+SKTAWRTDEEF 
Sbjct: 311 LYEGGIRLPQGPLFKALTDAIPLEMIRELLRTDGEGILRFPTPLVIKDSKTAWRTDEEFA 370

Query: 390 REMLAGVNPVIIRRLEEFPPASKLDPKAYGKQNSSITEGHIAQHLNGLTVDQALEMNKLF 449
           REMLAGVNP+II RL+EFPP SKLDP+AYG QNS+IT  HI   L+GLTVD+A+  NKLF
Sbjct: 371 REMLAGVNPIIISRLQEFPPKSKLDPEAYGNQNSTITAEHIEDKLDGLTVDEAMNNNKLF 430

Query: 450 ILDHHDSLMPYISRINSTSTKMYATRTVLLLQDNGILKPLAIELSLPHPQGDHHGAVSKV 509
           IL+HHD L+PY+ RIN+T+TK YA+RT+L LQDNG LKPLAIELSLPHP GD  G  SKV
Sbjct: 431 ILNHHDVLIPYLRRINTTTTKTYASRTLLFLQDNGSLKPLAIELSLPHPDGDQFGVTSKV 490

Query: 510 FTPAEHGVEGSVWQLAKAYVAVSDSGYHQLISHWLNTHAVIEPFVIATNRQLSVMHPIYK 569
           +TP++ GVE S+WQLAKAYVAV+DSG HQLISHWLNTHAVIEPFVIATNRQLSV+HPI+K
Sbjct: 491 YTPSDQGVESSIWQLAKAYVAVNDSGVHQLISHWLNTHAVIEPFVIATNRQLSVLHPIHK 550

Query: 570 LLHPHFRDTMNINAMARQVLINAGGILETTVFPGKYALEMSAVLYKNWIFTDQALPADLI 629
           LL+PHFRDTMNINAMARQ+LINAGG+LE+TVFP K+A+EMSAV+YK+W+F DQALPADL+
Sbjct: 551 LLYPHFRDTMNINAMARQILINAGGVLESTVFPSKFAMEMSAVVYKDWVFPDQALPADLV 610

Query: 630 KRGVAVPDPDSPHGLKLLIEDYPYAVDGLEIWSAIETWVREYCCFYYKSDEMVEEDTEIQ 689
           KRGVAV D  SPHG++LLIEDYPYAVDGLEIWSAI++WV +YC FYY SDE + +D E+Q
Sbjct: 611 KRGVAVEDSSSPHGVRLLIEDYPYAVDGLEIWSAIKSWVTDYCSFYYGSDEEILKDNELQ 670

Query: 690 SWWTELRNVGHGDLKDEPWWPKMKTREELIQSCTIVIWIASALHAAVNFGQYPYAGYLPN 749
           +WW ELR VGHGD K+EPWWP+M+T +ELI SCT +IWIASALHAAVNFGQYPYAGYLPN
Sbjct: 671 AWWKELREVGHGDKKNEPWWPEMETPQELIDSCTTIIWIASALHAAVNFGQYPYAGYLPN 730

Query: 750 RPTVSRRFMPEPGTPEFRELETDPDLAYLKTITAQLQTILGVSLIESLSRHSVDEIYLGQ 809
           RPTVSRRFMPEPGTPE+ EL+ +PD A+LKTITAQLQT+LGVSLIE LSRH+ DEIYLGQ
Sbjct: 731 RPTVSRRFMPEPGTPEYEELKKNPDKAFLKTITAQLQTLLGVSLIEILSRHTTDEIYLGQ 790

Query: 810 RETADWTSDEEAVAAFERFGDRLKEIEEKIMRMNNDERWRNRVGPVKMPYTLLFPNTSNY 869
           RE+ +WT D+E +AAF++FG +L +IE++I++ N D    NR GPV  PYTLLFP +   
Sbjct: 791 RESPEWTKDKEPLAAFDKFGKKLTDIEKQIIQRNGDNILINRSGPVNAPYTLLFPTS--- 850

Query: 870 YEEEGLNARGIPNSISI 885
             E GL  +GIPNS+SI
Sbjct: 851 --EGGLTGKGIPNSVSI 860

BLAST of Lcy01g002270 vs. ExPASy Swiss-Prot
Match: Q41238 (Linoleate 9S-lipoxygenase 6 (Fragment) OS=Solanum tuberosum OX=4113 GN=LOX1.6 PE=1 SV=1)

HSP 1 Score: 1196.8 bits (3095), Expect = 0.0e+00
Identity = 572/857 (66.74%), Postives = 692/857 (80.75%), Query Frame = 0

Query: 30  GGGGGGSRIRGTVVLMKKCVLDFKDVKASVLDRVHEFLGKGVSIQLISSDPSSSGDRLGA 89
           GG     +++GTVV+MKK  LDF D+  S+ D++ E LG+ VS QLISS  S   + L  
Sbjct: 8   GGHHDSKKVKGTVVMMKKNALDFTDLAGSLTDKIFEALGQKVSFQLISSVQSDPANGL-Q 67

Query: 90  AKVGKAANLEQWILTMKPSLTADDIEFTVFFEWEERMGVPGAFIIRNHHSSQFFLKTVTL 149
            K    A LE ++ T+ P L A +  F V F+W E  GVPGAFII+N H ++FFLK++TL
Sbjct: 68  GKHSNPAYLENFLFTLTP-LAAGETAFGVTFDWNEEFGVPGAFIIKNTHINEFFLKSLTL 127

Query: 150 HDVPGHGSVHFVCNSWVYPAHRYTYDRVFFSNKSYLPSETPEPLRKYREEELVNLRGNGI 209
            DVP HG VHFVCNSWVYP+ RY  DR+FF+N+ YLPSETPE LRKYRE EL+ LRG+G 
Sbjct: 128 EDVPNHGKVHFVCNSWVYPSFRYKSDRIFFANQPYLPSETPELLRKYRENELLTLRGDGT 187

Query: 210 GELKEWDRVYDYAYYNDLGMPDMGKRFVRTVLGGSKEFPYPRRGRTGRKPAKADPCCESR 269
           G+ + WDR+YDY  YNDLG PD G++ VRT LGGS ++PYPRRGRTGR P + DP  ESR
Sbjct: 188 GKREAWDRIYDYDVYNDLGNPDQGEQNVRTTLGGSADYPYPRRGRTGRPPTRTDPKSESR 247

Query: 270 LPL-LSLDIYVPRDERFRNLKFSDFLAYAVKSIFQTLVPEIKSLCDKTINEFDCFQDVLD 329
           +PL LSLDIYVPRDERF +LK SDFL YA+KSI Q ++PE+ +L D T NEFD F+DVL 
Sbjct: 248 IPLILSLDIYVPRDERFGHLKMSDFLTYALKSIVQFILPELHALFDGTPNEFDSFEDVLR 307

Query: 330 LYEGGMSLP-NEILGSLRELVPWQLFRELMRSDGRQFLKFPVPDVIKESKTAWRTDEEFG 389
           LYEGG+ LP   +  +L   +P ++ +EL+R+DG   L+FP P VIK+SKTAWRTDEEF 
Sbjct: 308 LYEGGIKLPQGPLFKALTAAIPLEMMKELLRTDGEGILRFPTPLVIKDSKTAWRTDEEFA 367

Query: 390 REMLAGVNPVIIRRLEEFPPASKLDPKAYGKQNSSITEGHIAQHLNGLTVDQALEMNKLF 449
           REMLAGVNP+II RL+EFPP SKLDP+AYG QNS+IT  HI   L+GLTVD+A+  NKLF
Sbjct: 368 REMLAGVNPIIISRLQEFPPKSKLDPEAYGNQNSTITAEHIEDKLDGLTVDEAMNNNKLF 427

Query: 450 ILDHHDSLMPYISRINSTSTKMYATRTVLLLQDNGILKPLAIELSLPHPQGDHHGAVSKV 509
           IL+HHD L+PY+ RIN+T+TK YA+RT+L LQDNG LKPLAIELSLPHP GD  G +SKV
Sbjct: 428 ILNHHDVLIPYLRRINTTTTKTYASRTLLFLQDNGSLKPLAIELSLPHPDGDQFGVISKV 487

Query: 510 FTPAEHGVEGSVWQLAKAYVAVSDSGYHQLISHWLNTHAVIEPFVIATNRQLSVMHPIYK 569
           +TP++ GVE S+WQLAKAYVAV+DSG HQLISHWLNTHAVIEPFVIATNRQLSV+HPI+K
Sbjct: 488 YTPSDQGVESSIWQLAKAYVAVNDSGVHQLISHWLNTHAVIEPFVIATNRQLSVLHPIHK 547

Query: 570 LLHPHFRDTMNINAMARQVLINAGGILETTVFPGKYALEMSAVLYKNWIFTDQALPADLI 629
           LL+PHFRDTMNINAMARQ+LINAGG+LE+TVFP K+A+EMSAV+YK+W+F DQALPADL+
Sbjct: 548 LLYPHFRDTMNINAMARQILINAGGVLESTVFPSKFAMEMSAVVYKDWVFPDQALPADLV 607

Query: 630 KRGVAVPDPDSPHGLKLLIEDYPYAVDGLEIWSAIETWVREYCCFYYKSDEMVEEDTEIQ 689
           KRGVAV D  SPHG++LLIEDYPYAVDGLEIWSAI++WV +YC FYY SDE + +D E+Q
Sbjct: 608 KRGVAVEDSSSPHGVRLLIEDYPYAVDGLEIWSAIKSWVTDYCSFYYGSDEEILKDNELQ 667

Query: 690 SWWTELRNVGHGDLKDEPWWPKMKTREELIQSCTIVIWIASALHAAVNFGQYPYAGYLPN 749
           +WW ELR VGHGD K+EPWWP+M+T +ELI SCT +IWIASALHAAVNFGQYPYAGYLPN
Sbjct: 668 AWWKELREVGHGDKKNEPWWPEMETPQELIDSCTTIIWIASALHAAVNFGQYPYAGYLPN 727

Query: 750 RPTVSRRFMPEPGTPEFRELETDPDLAYLKTITAQLQTILGVSLIESLSRHSVDEIYLGQ 809
           RPTVSRRFMPEPGTPE+ EL+ +PD A+LKTITAQLQT+LGVSLIE LSRH+ DEIYLGQ
Sbjct: 728 RPTVSRRFMPEPGTPEYEELKKNPDKAFLKTITAQLQTLLGVSLIEILSRHTTDEIYLGQ 787

Query: 810 RETADWTSDEEAVAAFERFGDRLKEIEEKIMRMNNDERWRNRVGPVKMPYTLLFPNTSNY 869
           RE+ +WT D+E +AAF++FG +L +IE++I++ N D    NR GPV  PYTLLFP +   
Sbjct: 788 RESPEWTKDKEPLAAFDKFGKKLTDIEKQIIQRNGDNILTNRSGPVNAPYTLLFPTS--- 847

Query: 870 YEEEGLNARGIPNSISI 885
             E GL  +GIPNS+SI
Sbjct: 848 --EGGLTGKGIPNSVSI 857

BLAST of Lcy01g002270 vs. ExPASy Swiss-Prot
Match: P38415 (Linoleate 9S-lipoxygenase A OS=Solanum lycopersicum OX=4081 GN=LOX1.1 PE=2 SV=1)

HSP 1 Score: 1196.4 bits (3094), Expect = 0.0e+00
Identity = 572/857 (66.74%), Postives = 692/857 (80.75%), Query Frame = 0

Query: 30  GGGGGGSRIRGTVVLMKKCVLDFKDVKASVLDRVHEFLGKGVSIQLISSDPSSSGDRLGA 89
           GG     +++GTVV+MKK  LDF D+  S+ D++ E LG+ VS QLISS  S   + L  
Sbjct: 11  GGHHDSKKVKGTVVMMKKNALDFTDLAGSLTDKIFEALGQKVSFQLISSVQSDPANGL-Q 70

Query: 90  AKVGKAANLEQWILTMKPSLTADDIEFTVFFEWEERMGVPGAFIIRNHHSSQFFLKTVTL 149
            K    A LE ++LT+ P L A +  F V F+W E  GVPGAF+I+N H ++FFLK++TL
Sbjct: 71  GKHSNPAYLENFLLTLTP-LAAGETAFGVTFDWNEEFGVPGAFVIKNMHINEFFLKSLTL 130

Query: 150 HDVPGHGSVHFVCNSWVYPAHRYTYDRVFFSNKSYLPSETPEPLRKYREEELVNLRGNGI 209
            DVP HG VHFVCNSWVYP+ RY  DR+FF+N+ YLPSETPE LRKYRE ELV LRG+G 
Sbjct: 131 EDVPNHGKVHFVCNSWVYPSFRYKSDRIFFANQPYLPSETPELLRKYRENELVTLRGDGT 190

Query: 210 GELKEWDRVYDYAYYNDLGMPDMGKRFVRTVLGGSKEFPYPRRGRTGRKPAKADPCCESR 269
           G+ + WDR+YDY  YNDLG PD GK  VRT LGGS ++PYPRRGRTGR P + DP  ESR
Sbjct: 191 GKREAWDRIYDYDVYNDLGNPDQGKENVRTTLGGSADYPYPRRGRTGRPPTRTDPKSESR 250

Query: 270 LPL-LSLDIYVPRDERFRNLKFSDFLAYAVKSIFQTLVPEIKSLCDKTINEFDCFQDVLD 329
           +PL LSLDIYVPRDERF +LK SDFL YA+KSI Q ++PE+ +L D T NEFD F+DVL 
Sbjct: 251 IPLILSLDIYVPRDERFGHLKMSDFLTYALKSIVQFILPELHALFDGTPNEFDSFEDVLR 310

Query: 330 LYEGGMSLP-NEILGSLRELVPWQLFRELMRSDGRQFLKFPVPDVIKESKTAWRTDEEFG 389
           LYEGG+ LP   +  +L + +P ++ REL+R+DG   L+FP P VIK+SKTAWRTDEEF 
Sbjct: 311 LYEGGIKLPQGPLFKALTDAIPLEMIRELLRTDGEGILRFPTPLVIKDSKTAWRTDEEFA 370

Query: 390 REMLAGVNPVIIRRLEEFPPASKLDPKAYGKQNSSITEGHIAQHLNGLTVDQALEMNKLF 449
           REMLAGVNPVII RLEEFPP SKLDP+ YG QNS+IT  HI   L+GLT+D+A+  NKLF
Sbjct: 371 REMLAGVNPVIISRLEEFPPKSKLDPELYGNQNSTITAEHIEGKLDGLTIDEAINSNKLF 430

Query: 450 ILDHHDSLMPYISRINSTSTKMYATRTVLLLQDNGILKPLAIELSLPHPQGDHHGAVSKV 509
           IL+HHD L+PY+ RIN+T+TK YA+RT+L LQDNG LKPLAIELSLPHP GD  G  SKV
Sbjct: 431 ILNHHDVLIPYLRRINTTTTKTYASRTLLFLQDNGSLKPLAIELSLPHPDGDQFGVTSKV 490

Query: 510 FTPAEHGVEGSVWQLAKAYVAVSDSGYHQLISHWLNTHAVIEPFVIATNRQLSVMHPIYK 569
           +TP++ GVEGS+WQLAKAYVAV+DSG HQLISHWLNTHAVIEPFVIATNRQLSV+HPI+K
Sbjct: 491 YTPSDQGVEGSIWQLAKAYVAVNDSGVHQLISHWLNTHAVIEPFVIATNRQLSVLHPIHK 550

Query: 570 LLHPHFRDTMNINAMARQVLINAGGILETTVFPGKYALEMSAVLYKNWIFTDQALPADLI 629
           LL+PHFRDTMNINA+ARQ+LINAGG+LE+TVFP K+A+EMSAV+YK+W+F DQALPADL+
Sbjct: 551 LLYPHFRDTMNINALARQILINAGGVLESTVFPSKFAMEMSAVVYKDWVFPDQALPADLV 610

Query: 630 KRGVAVPDPDSPHGLKLLIEDYPYAVDGLEIWSAIETWVREYCCFYYKSDEMVEEDTEIQ 689
           KRGVAV D  SPHG++LLI+DYPYAVDGLEIWSAI++WV +YC FYY S+E + +D E+Q
Sbjct: 611 KRGVAVEDSSSPHGVRLLIDDYPYAVDGLEIWSAIKSWVTDYCSFYYGSNEEILKDNELQ 670

Query: 690 SWWTELRNVGHGDLKDEPWWPKMKTREELIQSCTIVIWIASALHAAVNFGQYPYAGYLPN 749
           +WW E+R VGHGD K+EPWW +M+T +ELI SCT +IWIASALHAAVNFGQYPYAGYLPN
Sbjct: 671 AWWKEVREVGHGDKKNEPWWAEMETPQELIDSCTTIIWIASALHAAVNFGQYPYAGYLPN 730

Query: 750 RPTVSRRFMPEPGTPEFRELETDPDLAYLKTITAQLQTILGVSLIESLSRHSVDEIYLGQ 809
           RPTVSR+FMPEPGTPE+ EL+ +PD A+LKTITAQLQT+LGVSLIE LSRH+ DEIYLGQ
Sbjct: 731 RPTVSRKFMPEPGTPEYEELKKNPDKAFLKTITAQLQTLLGVSLIEILSRHTTDEIYLGQ 790

Query: 810 RETADWTSDEEAVAAFERFGDRLKEIEEKIMRMNNDERWRNRVGPVKMPYTLLFPNTSNY 869
           RE+ +WT D+E +AAFERFG++L +IE++IM+ N +    NR GPV  PYTLLFP +   
Sbjct: 791 RESPEWTKDKEPLAAFERFGNKLTDIEKQIMQRNGNNILTNRTGPVNAPYTLLFPTS--- 850

Query: 870 YEEEGLNARGIPNSISI 885
             E GL  +GIPNS+SI
Sbjct: 851 --EGGLTGKGIPNSVSI 860

BLAST of Lcy01g002270 vs. ExPASy TrEMBL
Match: A0A6J1EQD3 (Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111436857 PE=3 SV=1)

HSP 1 Score: 1688.3 bits (4371), Expect = 0.0e+00
Identity = 812/886 (91.65%), Postives = 848/886 (95.71%), Query Frame = 0

Query: 1   MGICPGVEMLQKMLEAFFSGNLMKFGYGGGGGGGGS--RIRGTVVLMKKCVLDFKDVKAS 60
           MGICPG+EMLQK+LEAFFS +LMKFGYGGGGGG G+  RIRGTVVLMKK VLDFKDVKAS
Sbjct: 1   MGICPGMEMLQKLLEAFFSSSLMKFGYGGGGGGSGNGRRIRGTVVLMKKSVLDFKDVKAS 60

Query: 61  VLDRVHEFLGKGVSIQLISSDPSSSGDRLGAAKVGKAANLEQWILTMKPSLTADDIEFTV 120
           V+DR+HEFLGKGVSIQLISSDP SS DRLG   VGKAA LEQWI T+KPSLTAD+IEFTV
Sbjct: 61  VVDRIHEFLGKGVSIQLISSDPPSSEDRLGVEMVGKAAMLEQWISTVKPSLTADEIEFTV 120

Query: 121 FFEWEERMGVPGAFIIRNHHSSQFFLKTVTLHDVPGHGSVHFVCNSWVYPAHRYTYDRVF 180
           FFEWEERMGVPGAFIIRNHHSSQF+LKTVTLHDVPG+GSVHFVCNSWVYPAHRYTYDRVF
Sbjct: 121 FFEWEERMGVPGAFIIRNHHSSQFYLKTVTLHDVPGYGSVHFVCNSWVYPAHRYTYDRVF 180

Query: 181 FSNKSYLPSETPEPLRKYREEELVNLRGNGIGELKEWDRVYDYAYYNDLGMPDMGKRFVR 240
           FSNKSYLPSETPEPLRKYRE EL+NLRGNG+GELKEWDRVYDYA YNDLGMPD  KRFVR
Sbjct: 181 FSNKSYLPSETPEPLRKYRENELINLRGNGVGELKEWDRVYDYACYNDLGMPDTSKRFVR 240

Query: 241 TVLGGSKEFPYPRRGRTGRKPAKADPCCESRLPLLSLDIYVPRDERFRNLKFSDFLAYAV 300
           TVLGGSKEFPYPRRGRTGRK AK+DP CESRLPLLSLDIYVPRDERFR+LK SDFLAYAV
Sbjct: 241 TVLGGSKEFPYPRRGRTGRKQAKSDPSCESRLPLLSLDIYVPRDERFRHLKLSDFLAYAV 300

Query: 301 KSIFQTLVPEIKSLCDKTINEFDCFQDVLDLYEGGMSLPNEILGSLRELVPWQLFRELMR 360
           KSI QTL+PEIKSLCDKTINEFDCFQDVLDLYEGGM LPNEILG+LR+LVPWQLFRELMR
Sbjct: 301 KSIVQTLLPEIKSLCDKTINEFDCFQDVLDLYEGGMRLPNEILGNLRDLVPWQLFRELMR 360

Query: 361 SDGRQFLKFPVPDVIKESKTAWRTDEEFGREMLAGVNPVIIRRLEEFPPASKLDPKAYGK 420
           SDG QFLKFPVPDVIKESKTAWRTDEEFGREMLAGVNPVIIRRL+EFPP SKLDPK YGK
Sbjct: 361 SDGHQFLKFPVPDVIKESKTAWRTDEEFGREMLAGVNPVIIRRLQEFPPTSKLDPKLYGK 420

Query: 421 QNSSITEGHIAQHLNGLTVDQALEMNKLFILDHHDSLMPYISRINSTSTKMYATRTVLLL 480
           QNSSITE HIA+HLNGLTVDQALEMNKLFILDHHDSLMPYISRINSTSTK YATRT+L+L
Sbjct: 421 QNSSITEEHIAKHLNGLTVDQALEMNKLFILDHHDSLMPYISRINSTSTKTYATRTLLVL 480

Query: 481 QDNGILKPLAIELSLPHPQGDHHGAVSKVFTPAEHGVEGSVWQLAKAYVAVSDSGYHQLI 540
           QDNGILKP+AIELSLPHPQGDHHGAVSKVFTPAE+G+EGSVWQLAKAYVAV+DSGYHQLI
Sbjct: 481 QDNGILKPIAIELSLPHPQGDHHGAVSKVFTPAENGIEGSVWQLAKAYVAVNDSGYHQLI 540

Query: 541 SHWLNTHAVIEPFVIATNRQLSVMHPIYKLLHPHFRDTMNINAMARQVLINAGGILETTV 600
           SHWLNTHAVIEPF+IATNR LSVMHPIYKLLHPHFRDTMNINAMARQVLINAGGILE TV
Sbjct: 541 SHWLNTHAVIEPFIIATNRHLSVMHPIYKLLHPHFRDTMNINAMARQVLINAGGILEITV 600

Query: 601 FPGKYALEMSAVLYKNWIFTDQALPADLIKRGVAVPDPDSPHGLKLLIEDYPYAVDGLEI 660
           FPGKYALEMSAVLYKNW+FTDQALPADLIKRGVAVPDP S HGLKLLIEDYPYAVDGLEI
Sbjct: 601 FPGKYALEMSAVLYKNWVFTDQALPADLIKRGVAVPDPSSSHGLKLLIEDYPYAVDGLEI 660

Query: 661 WSAIETWVREYCCFYYKSDEMVEEDTEIQSWWTELRNVGHGDLKDEPWWPKMKTREELIQ 720
           WSAIETWVREY  FYYKSDEMV +DTEIQSWWTELRNVGHGDLKDEPWWPKM TRE+LIQ
Sbjct: 661 WSAIETWVREYSYFYYKSDEMVVQDTEIQSWWTELRNVGHGDLKDEPWWPKMNTREDLIQ 720

Query: 721 SCTIVIWIASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEFRELETDPDLAYLKT 780
           SC  +IWIASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEFRELETDPDLAYLKT
Sbjct: 721 SCATIIWIASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEFRELETDPDLAYLKT 780

Query: 781 ITAQLQTILGVSLIESLSRHSVDEIYLGQRETADWTSDEEAVAAFERFGDRLKEIEEKIM 840
           ITAQLQTILGVSLIESLSRHSVDEIYLGQR+TADWT+DEE +AAFERFGDRL+EIEEKI+
Sbjct: 781 ITAQLQTILGVSLIESLSRHSVDEIYLGQRDTADWTTDEEPMAAFERFGDRLREIEEKIL 840

Query: 841 RMNNDERWRNRVGPVKMPYTLLFPNTSNYYEEEGLNARGIPNSISI 885
            MNNDERWRNRVGPVKMPYTLLFPNTSN+YEEEGLNARGIPNSISI
Sbjct: 841 SMNNDERWRNRVGPVKMPYTLLFPNTSNFYEEEGLNARGIPNSISI 886

BLAST of Lcy01g002270 vs. ExPASy TrEMBL
Match: A0A6J1KC57 (Lipoxygenase OS=Cucurbita maxima OX=3661 GN=LOC111494274 PE=3 SV=1)

HSP 1 Score: 1676.8 bits (4341), Expect = 0.0e+00
Identity = 807/886 (91.08%), Postives = 845/886 (95.37%), Query Frame = 0

Query: 1   MGICPGVEMLQKMLEAFFSGNLMKFGYGGGGGGGGS--RIRGTVVLMKKCVLDFKDVKAS 60
           MGICPG+EMLQK+LEAFFS  LMKFGYG GGGG G+  RIRGTVVLMKK VLDFKDVKAS
Sbjct: 1   MGICPGMEMLQKLLEAFFSRCLMKFGYGDGGGGSGNGRRIRGTVVLMKKSVLDFKDVKAS 60

Query: 61  VLDRVHEFLGKGVSIQLISSDPSSSGDRLGAAKVGKAANLEQWILTMKPSLTADDIEFTV 120
           V+DR+HEFLGKGVSIQLISSD  +S DRLG   VGKAA LEQWI T+KPSLTAD+IEFTV
Sbjct: 61  VVDRIHEFLGKGVSIQLISSDLPASEDRLGVEMVGKAAMLEQWISTVKPSLTADEIEFTV 120

Query: 121 FFEWEERMGVPGAFIIRNHHSSQFFLKTVTLHDVPGHGSVHFVCNSWVYPAHRYTYDRVF 180
           FFEWEERMGVPGAFIIRNHHSSQF+LKTVTLHDVPG+GSVHFVCNSWVYPAHRYTYDRVF
Sbjct: 121 FFEWEERMGVPGAFIIRNHHSSQFYLKTVTLHDVPGYGSVHFVCNSWVYPAHRYTYDRVF 180

Query: 181 FSNKSYLPSETPEPLRKYREEELVNLRGNGIGELKEWDRVYDYAYYNDLGMPDMGKRFVR 240
           FSNKSYLPS+TPEPLRKYRE EL+NLRGNG+GELKEWDRVYDYA YNDLGMPDM KRFVR
Sbjct: 181 FSNKSYLPSKTPEPLRKYRENELINLRGNGVGELKEWDRVYDYACYNDLGMPDMSKRFVR 240

Query: 241 TVLGGSKEFPYPRRGRTGRKPAKADPCCESRLPLLSLDIYVPRDERFRNLKFSDFLAYAV 300
           TVLGGSKEFPYPRRGRTGRK AK+DP CESRL LLSLDIYVPRDERF +LK SDFLAYAV
Sbjct: 241 TVLGGSKEFPYPRRGRTGRKQAKSDPSCESRLQLLSLDIYVPRDERFCHLKLSDFLAYAV 300

Query: 301 KSIFQTLVPEIKSLCDKTINEFDCFQDVLDLYEGGMSLPNEILGSLRELVPWQLFRELMR 360
           KSI QTL+PEI+SLCDKTINEFDCFQDVLDLYEGGM LPNEILG+LR+LVPWQLFRELMR
Sbjct: 301 KSIVQTLLPEIRSLCDKTINEFDCFQDVLDLYEGGMRLPNEILGNLRDLVPWQLFRELMR 360

Query: 361 SDGRQFLKFPVPDVIKESKTAWRTDEEFGREMLAGVNPVIIRRLEEFPPASKLDPKAYGK 420
           SDG QFLKFPVPDVIKESKTAWRTDEEFGREMLAGVNPVIIRRL+EFPP SKLDPK YGK
Sbjct: 361 SDGHQFLKFPVPDVIKESKTAWRTDEEFGREMLAGVNPVIIRRLQEFPPTSKLDPKLYGK 420

Query: 421 QNSSITEGHIAQHLNGLTVDQALEMNKLFILDHHDSLMPYISRINSTSTKMYATRTVLLL 480
           QNSSITE HIA+HLNGLTVDQALEMNKLFILDHHDSLMPYISRINSTSTK YATRT+L+L
Sbjct: 421 QNSSITEEHIAKHLNGLTVDQALEMNKLFILDHHDSLMPYISRINSTSTKTYATRTLLVL 480

Query: 481 QDNGILKPLAIELSLPHPQGDHHGAVSKVFTPAEHGVEGSVWQLAKAYVAVSDSGYHQLI 540
           QDNGILKP+AIELSLPHPQGDHHGAVSKVFTPAE+G+EGSVWQLAKAYVAV+DSGYHQLI
Sbjct: 481 QDNGILKPIAIELSLPHPQGDHHGAVSKVFTPAENGIEGSVWQLAKAYVAVNDSGYHQLI 540

Query: 541 SHWLNTHAVIEPFVIATNRQLSVMHPIYKLLHPHFRDTMNINAMARQVLINAGGILETTV 600
           SHWLNTHAVIEPF+IATNR LSVMHPIYKLLHPHFRDTMNINAMARQVLINAGGILE TV
Sbjct: 541 SHWLNTHAVIEPFIIATNRHLSVMHPIYKLLHPHFRDTMNINAMARQVLINAGGILEITV 600

Query: 601 FPGKYALEMSAVLYKNWIFTDQALPADLIKRGVAVPDPDSPHGLKLLIEDYPYAVDGLEI 660
           FPGKYALEMSAVLYKNW+FTDQALPADLI RGVAVPDP S HGLKLLIEDYPYAVDGLEI
Sbjct: 601 FPGKYALEMSAVLYKNWVFTDQALPADLINRGVAVPDPSSSHGLKLLIEDYPYAVDGLEI 660

Query: 661 WSAIETWVREYCCFYYKSDEMVEEDTEIQSWWTELRNVGHGDLKDEPWWPKMKTREELIQ 720
           WSAIETWVREY CFYYKSDEMV++DTEIQSWWTELRNVGHGDLKDEPWWPKM TRE+LIQ
Sbjct: 661 WSAIETWVREYSCFYYKSDEMVQQDTEIQSWWTELRNVGHGDLKDEPWWPKMNTREDLIQ 720

Query: 721 SCTIVIWIASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEFRELETDPDLAYLKT 780
           SC  +IWIASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEFRELETDPDLAYLKT
Sbjct: 721 SCATIIWIASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEFRELETDPDLAYLKT 780

Query: 781 ITAQLQTILGVSLIESLSRHSVDEIYLGQRETADWTSDEEAVAAFERFGDRLKEIEEKIM 840
           ITAQLQTILGVSLIESLSRHSVDEIYLGQR+TADWT+DEE +AAFERFGDRL+EIEEKIM
Sbjct: 781 ITAQLQTILGVSLIESLSRHSVDEIYLGQRDTADWTTDEEPMAAFERFGDRLREIEEKIM 840

Query: 841 RMNNDERWRNRVGPVKMPYTLLFPNTSNYYEEEGLNARGIPNSISI 885
            MNNDERWRNRVGPVKMPYTLLFPNTSN+YEEEGLNARGIPNSISI
Sbjct: 841 SMNNDERWRNRVGPVKMPYTLLFPNTSNFYEEEGLNARGIPNSISI 886

BLAST of Lcy01g002270 vs. ExPASy TrEMBL
Match: Q9M463 (Lipoxygenase OS=Cucumis sativus OX=3659 GN=lox9 PE=2 SV=1)

HSP 1 Score: 1664.8 bits (4310), Expect = 0.0e+00
Identity = 795/884 (89.93%), Postives = 844/884 (95.48%), Query Frame = 0

Query: 1   MGICPGVEMLQKMLEAFFSGNLMKFGYGGGGGGGGSRIRGTVVLMKKCVLDFKDVKASVL 60
           MGICPGVEMLQK+LEAFFSG+LMKFGYGGGGGG  +RIRGTVV+MKKCVLDFKDVKASVL
Sbjct: 1   MGICPGVEMLQKLLEAFFSGSLMKFGYGGGGGGDRTRIRGTVVIMKKCVLDFKDVKASVL 60

Query: 61  DRVHEFLGKGVSIQLISSDPSSSGDRLGAAKVGKAANLEQWILTMKPSLTADDIEFTVFF 120
           DRVHEF GKGV+IQLISS+P  SG RLGA  VGKAANLEQWI T+KPSLTADDIE T+FF
Sbjct: 61  DRVHEFFGKGVTIQLISSNPPQSG-RLGA--VGKAANLEQWISTVKPSLTADDIELTIFF 120

Query: 121 EWEERMGVPGAFIIRNHHSSQFFLKTVTLHDVPGHGSVHFVCNSWVYPAHRYTYDRVFFS 180
           EWEERMGVPGAF IRNHH +QF+LKTVTLHDVPG+GSVHFVCNSWVYPAHRYTYDRVFFS
Sbjct: 121 EWEERMGVPGAFTIRNHHPNQFYLKTVTLHDVPGYGSVHFVCNSWVYPAHRYTYDRVFFS 180

Query: 181 NKSYLPSETPEPLRKYREEELVNLRGNGIGELKEWDRVYDYAYYNDLGMPDMGKRFVRTV 240
           NKSYLPSETPE LRKYREEELVNLRG+  GELKEWDRVYDYA YNDLGMPD GK++VRT 
Sbjct: 181 NKSYLPSETPESLRKYREEELVNLRGDNRGELKEWDRVYDYACYNDLGMPDSGKKYVRTS 240

Query: 241 LGGSKEFPYPRRGRTGRKPAKADPCCESRLPLLSLDIYVPRDERFRNLKFSDFLAYAVKS 300
           LGG++EFPYPRRGRTGRKPAKADP CE RLPLLSLDIYVPRDERFRNLK SDFLAY+VKS
Sbjct: 241 LGGTREFPYPRRGRTGRKPAKADPSCEHRLPLLSLDIYVPRDERFRNLKLSDFLAYSVKS 300

Query: 301 IFQTLVPEIKSLCDKTINEFDCFQDVLDLYEGGMSLPNEILGSLRELVPWQLFRELMRSD 360
           + Q+L+PEIKSLCDKTINEFDCFQDVLDLYEGGMSLP E+LG+LRELVPWQLFRELMRSD
Sbjct: 301 VVQSLLPEIKSLCDKTINEFDCFQDVLDLYEGGMSLPTEVLGTLRELVPWQLFRELMRSD 360

Query: 361 GRQFLKFPVPDVIKESKTAWRTDEEFGREMLAGVNPVIIRRLEEFPPASKLDPKAYGKQN 420
           GRQFLKFPVPDVIKE+KTAWRTDEEFGREMLAGVNPVIIRRL EFPP SKLDPK YGKQN
Sbjct: 361 GRQFLKFPVPDVIKENKTAWRTDEEFGREMLAGVNPVIIRRLLEFPPVSKLDPKTYGKQN 420

Query: 421 SSITEGHIAQHLNGLTVDQALEMNKLFILDHHDSLMPYISRINSTSTKMYATRTVLLLQD 480
           SSITE HIA+HLNGLT+DQALEMNKLFILDHHD+LMPYISRINSTSTK YATRT+LLLQD
Sbjct: 421 SSITEEHIAEHLNGLTIDQALEMNKLFILDHHDALMPYISRINSTSTKTYATRTLLLLQD 480

Query: 481 NGILKPLAIELSLPHPQGDHHGAVSKVFTPAEHGVEGSVWQLAKAYVAVSDSGYHQLISH 540
           NGILKPLAIELSLPHPQG+HHG+VSKVFTPAEHGVEGSVWQLAKAYVAV+DSGYHQLISH
Sbjct: 481 NGILKPLAIELSLPHPQGEHHGSVSKVFTPAEHGVEGSVWQLAKAYVAVNDSGYHQLISH 540

Query: 541 WLNTHAVIEPFVIATNRQLSVMHPIYKLLHPHFRDTMNINAMARQVLINAGGILETTVFP 600
           WLNTHAVIEPF+I TNRQLSV+HPIYKLLHPHFRDTMNINAMARQVLINAGGILETTVFP
Sbjct: 541 WLNTHAVIEPFIIGTNRQLSVVHPIYKLLHPHFRDTMNINAMARQVLINAGGILETTVFP 600

Query: 601 GKYALEMSAVLYKNWIFTDQALPADLIKRGVAVPDPDSPHGLKLLIEDYPYAVDGLEIWS 660
           GKYALEMSAV+YKNW+FTDQA PADLIKRGVA+PD  SPHGLKLLIEDYPYAVDGLEIWS
Sbjct: 601 GKYALEMSAVIYKNWVFTDQAHPADLIKRGVAIPDSSSPHGLKLLIEDYPYAVDGLEIWS 660

Query: 661 AIETWVREYCCFYYKSDEMVEEDTEIQSWWTELRNVGHGDLKDEPWWPKMKTREELIQSC 720
           AIE WVR+Y  FYYKSDEMV++DTEIQSWWTE+R VGHGDLKDEPWWPKM TRE+L+QSC
Sbjct: 661 AIEKWVRDYSYFYYKSDEMVQKDTEIQSWWTEIRTVGHGDLKDEPWWPKMNTREDLVQSC 720

Query: 721 TIVIWIASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEFRELETDPDLAYLKTIT 780
           TI+IWIASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEFRELETDP+LAYLKTIT
Sbjct: 721 TIIIWIASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEFRELETDPELAYLKTIT 780

Query: 781 AQLQTILGVSLIESLSRHSVDEIYLGQRETADWTSDEEAVAAFERFGDRLKEIEEKIMRM 840
           AQLQTILGVSLIESLSRHSVDEIYLGQR+T +WT DEEA+AAFERFGDRL+EIEEKIMRM
Sbjct: 781 AQLQTILGVSLIESLSRHSVDEIYLGQRDTPEWTKDEEALAAFERFGDRLREIEEKIMRM 840

Query: 841 NNDERWRNRVGPVKMPYTLLFPNTSNYYEEEGLNARGIPNSISI 885
           NN+E+WRNRVGPVKMPYT LFPNTSNYYEEEGLNARGIPNSISI
Sbjct: 841 NNEEKWRNRVGPVKMPYTFLFPNTSNYYEEEGLNARGIPNSISI 881

BLAST of Lcy01g002270 vs. ExPASy TrEMBL
Match: A0A5D3DC72 (Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold75860G00450 PE=3 SV=1)

HSP 1 Score: 1656.7 bits (4289), Expect = 0.0e+00
Identity = 794/884 (89.82%), Postives = 839/884 (94.91%), Query Frame = 0

Query: 1   MGICPGVEMLQKMLEAFFSGNLMKFGYGGGGGGGGSRIRGTVVLMKKCVLDFKDVKASVL 60
           MGICPGVEMLQK+LEAFFSGNLMKFGYGGG GG   RIRGTVV+MKKCVLDFKDV+ASVL
Sbjct: 1   MGICPGVEMLQKLLEAFFSGNLMKFGYGGGSGGDRKRIRGTVVIMKKCVLDFKDVRASVL 60

Query: 61  DRVHEFLGKGVSIQLISSDPSSSGDRLGAAKVGKAANLEQWILTMKPSLTADDIEFTVFF 120
           DRVHEF GKGV+I+LISS+P  SG RLGA  VGK ANLEQWI T+KPSLTADDIE T+FF
Sbjct: 61  DRVHEFFGKGVTIELISSNPPQSG-RLGA--VGKGANLEQWISTVKPSLTADDIELTIFF 120

Query: 121 EWEERMGVPGAFIIRNHHSSQFFLKTVTLHDVPGHGSVHFVCNSWVYPAHRYTYDRVFFS 180
           EWEERMGVPGAF+IRN+H +QF+LKTVTLHDVPG+GSVHFVCNSWVYPAHRYTYDRVFFS
Sbjct: 121 EWEERMGVPGAFMIRNNHPNQFYLKTVTLHDVPGYGSVHFVCNSWVYPAHRYTYDRVFFS 180

Query: 181 NKSYLPSETPEPLRKYREEELVNLRGNGIGELKEWDRVYDYAYYNDLGMPDMGKRFVRTV 240
           NKSYLPSETPE LRKYREEELVNLRGN  GELKEWDRVYDYA YNDLGMPD GK++VRT 
Sbjct: 181 NKSYLPSETPESLRKYREEELVNLRGNDKGELKEWDRVYDYACYNDLGMPDSGKKYVRTS 240

Query: 241 LGGSKEFPYPRRGRTGRKPAKADPCCESRLPLLSLDIYVPRDERFRNLKFSDFLAYAVKS 300
           LGGSKEFPYPRRGRTGRKPAKADP CE RLPLLSLDIYVPRDERFRNLK SDFLAY+VKS
Sbjct: 241 LGGSKEFPYPRRGRTGRKPAKADPSCEHRLPLLSLDIYVPRDERFRNLKLSDFLAYSVKS 300

Query: 301 IFQTLVPEIKSLCDKTINEFDCFQDVLDLYEGGMSLPNEILGSLRELVPWQLFRELMRSD 360
           I Q+L+PEIKSLCDKTINEFD FQDVLDLYEGGM+LP EIL +LRELVPWQL RELMRSD
Sbjct: 301 IVQSLLPEIKSLCDKTINEFDSFQDVLDLYEGGMTLPTEILATLRELVPWQLLRELMRSD 360

Query: 361 GRQFLKFPVPDVIKESKTAWRTDEEFGREMLAGVNPVIIRRLEEFPPASKLDPKAYGKQN 420
           GRQFLKFPVPDVIKE+KTAWRTDEEFGREMLAGVNPVIIRRL EFPP SKLDPK YGKQN
Sbjct: 361 GRQFLKFPVPDVIKENKTAWRTDEEFGREMLAGVNPVIIRRLLEFPPVSKLDPKTYGKQN 420

Query: 421 SSITEGHIAQHLNGLTVDQALEMNKLFILDHHDSLMPYISRINSTSTKMYATRTVLLLQD 480
           SSITE HIA HLNGLT+DQALEMNKLFILDHHD+LMPYISRINST+TK YATRT+LLLQD
Sbjct: 421 SSITEEHIAAHLNGLTIDQALEMNKLFILDHHDALMPYISRINSTATKTYATRTLLLLQD 480

Query: 481 NGILKPLAIELSLPHPQGDHHGAVSKVFTPAEHGVEGSVWQLAKAYVAVSDSGYHQLISH 540
           NGILKPLAIELSLPHPQGDHHG+VSKVFTPAEHGVEGSVWQLAKAYVAV+DSGYHQLISH
Sbjct: 481 NGILKPLAIELSLPHPQGDHHGSVSKVFTPAEHGVEGSVWQLAKAYVAVNDSGYHQLISH 540

Query: 541 WLNTHAVIEPFVIATNRQLSVMHPIYKLLHPHFRDTMNINAMARQVLINAGGILETTVFP 600
           WLNTHAVIEPF+I TNRQLSV+HPIYKLLHPHFRDTMNINAMARQVLINAGGILETTVFP
Sbjct: 541 WLNTHAVIEPFIIGTNRQLSVVHPIYKLLHPHFRDTMNINAMARQVLINAGGILETTVFP 600

Query: 601 GKYALEMSAVLYKNWIFTDQALPADLIKRGVAVPDPDSPHGLKLLIEDYPYAVDGLEIWS 660
           GKYALEMSAVLYKNW+FTDQALPADLIKRGVA+PDP SPHGLKLLIEDYP+AVDGLEIWS
Sbjct: 601 GKYALEMSAVLYKNWVFTDQALPADLIKRGVAIPDPSSPHGLKLLIEDYPFAVDGLEIWS 660

Query: 661 AIETWVREYCCFYYKSDEMVEEDTEIQSWWTELRNVGHGDLKDEPWWPKMKTREELIQSC 720
           AIE WVR+Y   YYKSDEMV+EDTEIQSWWTE+R VGHGDLKDEPWWPKM TRE+L+QSC
Sbjct: 661 AIEKWVRDYSYLYYKSDEMVQEDTEIQSWWTEIRTVGHGDLKDEPWWPKMNTREDLVQSC 720

Query: 721 TIVIWIASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEFRELETDPDLAYLKTIT 780
           TI+IWIASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEFRELETDP+LAYLKTIT
Sbjct: 721 TIIIWIASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEFRELETDPELAYLKTIT 780

Query: 781 AQLQTILGVSLIESLSRHSVDEIYLGQRETADWTSDEEAVAAFERFGDRLKEIEEKIMRM 840
           AQLQTILGVSLIESLSRHSVDEIYLGQR+T +WT DEEA+AAFERFGDRL+EIEEKIMRM
Sbjct: 781 AQLQTILGVSLIESLSRHSVDEIYLGQRDTPEWTKDEEALAAFERFGDRLREIEEKIMRM 840

Query: 841 NNDERWRNRVGPVKMPYTLLFPNTSNYYEEEGLNARGIPNSISI 885
           NN+E+WRNRVGPVKMPYT LFPNTSNYYEEEGLNARGIPNSISI
Sbjct: 841 NNEEKWRNRVGPVKMPYTFLFPNTSNYYEEEGLNARGIPNSISI 881

BLAST of Lcy01g002270 vs. ExPASy TrEMBL
Match: A0A1S3BMA3 (Lipoxygenase OS=Cucumis melo OX=3656 GN=LOC103491403 PE=3 SV=1)

HSP 1 Score: 1656.7 bits (4289), Expect = 0.0e+00
Identity = 794/884 (89.82%), Postives = 839/884 (94.91%), Query Frame = 0

Query: 1   MGICPGVEMLQKMLEAFFSGNLMKFGYGGGGGGGGSRIRGTVVLMKKCVLDFKDVKASVL 60
           MGICPGVEMLQK+LEAFFSGNLMKFGYGGG GG   RIRGTVV+MKKCVLDFKDV+ASVL
Sbjct: 1   MGICPGVEMLQKLLEAFFSGNLMKFGYGGGSGGDRKRIRGTVVIMKKCVLDFKDVRASVL 60

Query: 61  DRVHEFLGKGVSIQLISSDPSSSGDRLGAAKVGKAANLEQWILTMKPSLTADDIEFTVFF 120
           DRVHEF GKGV+I+LISS+P  SG RLGA  VGK ANLEQWI T+KPSLTADDIE T+FF
Sbjct: 61  DRVHEFFGKGVTIELISSNPPQSG-RLGA--VGKGANLEQWISTVKPSLTADDIELTIFF 120

Query: 121 EWEERMGVPGAFIIRNHHSSQFFLKTVTLHDVPGHGSVHFVCNSWVYPAHRYTYDRVFFS 180
           EWEERMGVPGAF+IRN+H +QF+LKTVTLHDVPG+GSVHFVCNSWVYPAHRYTYDRVFFS
Sbjct: 121 EWEERMGVPGAFMIRNNHPNQFYLKTVTLHDVPGYGSVHFVCNSWVYPAHRYTYDRVFFS 180

Query: 181 NKSYLPSETPEPLRKYREEELVNLRGNGIGELKEWDRVYDYAYYNDLGMPDMGKRFVRTV 240
           NKSYLPSETPE LRKYREEELVNLRGN  GELKEWDRVYDYA YNDLGMPD GK++VRT 
Sbjct: 181 NKSYLPSETPESLRKYREEELVNLRGNDKGELKEWDRVYDYACYNDLGMPDSGKKYVRTS 240

Query: 241 LGGSKEFPYPRRGRTGRKPAKADPCCESRLPLLSLDIYVPRDERFRNLKFSDFLAYAVKS 300
           LGGSKEFPYPRRGRTGRKPAKADP CE RLPLLSLDIYVPRDERFRNLK SDFLAY+VKS
Sbjct: 241 LGGSKEFPYPRRGRTGRKPAKADPSCEHRLPLLSLDIYVPRDERFRNLKLSDFLAYSVKS 300

Query: 301 IFQTLVPEIKSLCDKTINEFDCFQDVLDLYEGGMSLPNEILGSLRELVPWQLFRELMRSD 360
           I Q+L+PEIKSLCDKTINEFD FQDVLDLYEGGM+LP EIL +LRELVPWQL RELMRSD
Sbjct: 301 IVQSLLPEIKSLCDKTINEFDSFQDVLDLYEGGMTLPTEILATLRELVPWQLLRELMRSD 360

Query: 361 GRQFLKFPVPDVIKESKTAWRTDEEFGREMLAGVNPVIIRRLEEFPPASKLDPKAYGKQN 420
           GRQFLKFPVPDVIKE+KTAWRTDEEFGREMLAGVNPVIIRRL EFPP SKLDPK YGKQN
Sbjct: 361 GRQFLKFPVPDVIKENKTAWRTDEEFGREMLAGVNPVIIRRLLEFPPVSKLDPKTYGKQN 420

Query: 421 SSITEGHIAQHLNGLTVDQALEMNKLFILDHHDSLMPYISRINSTSTKMYATRTVLLLQD 480
           SSITE HIA HLNGLT+DQALEMNKLFILDHHD+LMPYISRINST+TK YATRT+LLLQD
Sbjct: 421 SSITEEHIAAHLNGLTIDQALEMNKLFILDHHDALMPYISRINSTATKTYATRTLLLLQD 480

Query: 481 NGILKPLAIELSLPHPQGDHHGAVSKVFTPAEHGVEGSVWQLAKAYVAVSDSGYHQLISH 540
           NGILKPLAIELSLPHPQGDHHG+VSKVFTPAEHGVEGSVWQLAKAYVAV+DSGYHQLISH
Sbjct: 481 NGILKPLAIELSLPHPQGDHHGSVSKVFTPAEHGVEGSVWQLAKAYVAVNDSGYHQLISH 540

Query: 541 WLNTHAVIEPFVIATNRQLSVMHPIYKLLHPHFRDTMNINAMARQVLINAGGILETTVFP 600
           WLNTHAVIEPF+I TNRQLSV+HPIYKLLHPHFRDTMNINAMARQVLINAGGILETTVFP
Sbjct: 541 WLNTHAVIEPFIIGTNRQLSVVHPIYKLLHPHFRDTMNINAMARQVLINAGGILETTVFP 600

Query: 601 GKYALEMSAVLYKNWIFTDQALPADLIKRGVAVPDPDSPHGLKLLIEDYPYAVDGLEIWS 660
           GKYALEMSAVLYKNW+FTDQALPADLIKRGVA+PDP SPHGLKLLIEDYP+AVDGLEIWS
Sbjct: 601 GKYALEMSAVLYKNWVFTDQALPADLIKRGVAIPDPSSPHGLKLLIEDYPFAVDGLEIWS 660

Query: 661 AIETWVREYCCFYYKSDEMVEEDTEIQSWWTELRNVGHGDLKDEPWWPKMKTREELIQSC 720
           AIE WVR+Y   YYKSDEMV+EDTEIQSWWTE+R VGHGDLKDEPWWPKM TRE+L+QSC
Sbjct: 661 AIEKWVRDYSYLYYKSDEMVQEDTEIQSWWTEIRTVGHGDLKDEPWWPKMNTREDLVQSC 720

Query: 721 TIVIWIASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEFRELETDPDLAYLKTIT 780
           TI+IWIASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEFRELETDP+LAYLKTIT
Sbjct: 721 TIIIWIASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEFRELETDPELAYLKTIT 780

Query: 781 AQLQTILGVSLIESLSRHSVDEIYLGQRETADWTSDEEAVAAFERFGDRLKEIEEKIMRM 840
           AQLQTILGVSLIESLSRHSVDEIYLGQR+T +WT DEEA+AAFERFGDRL+EIEEKIMRM
Sbjct: 781 AQLQTILGVSLIESLSRHSVDEIYLGQRDTPEWTKDEEALAAFERFGDRLREIEEKIMRM 840

Query: 841 NNDERWRNRVGPVKMPYTLLFPNTSNYYEEEGLNARGIPNSISI 885
           NN+E+WRNRVGPVKMPYT LFPNTSNYYEEEGLNARGIPNSISI
Sbjct: 841 NNEEKWRNRVGPVKMPYTFLFPNTSNYYEEEGLNARGIPNSISI 881

BLAST of Lcy01g002270 vs. NCBI nr
Match: XP_022930397.1 (probable linoleate 9S-lipoxygenase 5 [Cucurbita moschata])

HSP 1 Score: 1688.3 bits (4371), Expect = 0.0e+00
Identity = 812/886 (91.65%), Postives = 848/886 (95.71%), Query Frame = 0

Query: 1   MGICPGVEMLQKMLEAFFSGNLMKFGYGGGGGGGGS--RIRGTVVLMKKCVLDFKDVKAS 60
           MGICPG+EMLQK+LEAFFS +LMKFGYGGGGGG G+  RIRGTVVLMKK VLDFKDVKAS
Sbjct: 1   MGICPGMEMLQKLLEAFFSSSLMKFGYGGGGGGSGNGRRIRGTVVLMKKSVLDFKDVKAS 60

Query: 61  VLDRVHEFLGKGVSIQLISSDPSSSGDRLGAAKVGKAANLEQWILTMKPSLTADDIEFTV 120
           V+DR+HEFLGKGVSIQLISSDP SS DRLG   VGKAA LEQWI T+KPSLTAD+IEFTV
Sbjct: 61  VVDRIHEFLGKGVSIQLISSDPPSSEDRLGVEMVGKAAMLEQWISTVKPSLTADEIEFTV 120

Query: 121 FFEWEERMGVPGAFIIRNHHSSQFFLKTVTLHDVPGHGSVHFVCNSWVYPAHRYTYDRVF 180
           FFEWEERMGVPGAFIIRNHHSSQF+LKTVTLHDVPG+GSVHFVCNSWVYPAHRYTYDRVF
Sbjct: 121 FFEWEERMGVPGAFIIRNHHSSQFYLKTVTLHDVPGYGSVHFVCNSWVYPAHRYTYDRVF 180

Query: 181 FSNKSYLPSETPEPLRKYREEELVNLRGNGIGELKEWDRVYDYAYYNDLGMPDMGKRFVR 240
           FSNKSYLPSETPEPLRKYRE EL+NLRGNG+GELKEWDRVYDYA YNDLGMPD  KRFVR
Sbjct: 181 FSNKSYLPSETPEPLRKYRENELINLRGNGVGELKEWDRVYDYACYNDLGMPDTSKRFVR 240

Query: 241 TVLGGSKEFPYPRRGRTGRKPAKADPCCESRLPLLSLDIYVPRDERFRNLKFSDFLAYAV 300
           TVLGGSKEFPYPRRGRTGRK AK+DP CESRLPLLSLDIYVPRDERFR+LK SDFLAYAV
Sbjct: 241 TVLGGSKEFPYPRRGRTGRKQAKSDPSCESRLPLLSLDIYVPRDERFRHLKLSDFLAYAV 300

Query: 301 KSIFQTLVPEIKSLCDKTINEFDCFQDVLDLYEGGMSLPNEILGSLRELVPWQLFRELMR 360
           KSI QTL+PEIKSLCDKTINEFDCFQDVLDLYEGGM LPNEILG+LR+LVPWQLFRELMR
Sbjct: 301 KSIVQTLLPEIKSLCDKTINEFDCFQDVLDLYEGGMRLPNEILGNLRDLVPWQLFRELMR 360

Query: 361 SDGRQFLKFPVPDVIKESKTAWRTDEEFGREMLAGVNPVIIRRLEEFPPASKLDPKAYGK 420
           SDG QFLKFPVPDVIKESKTAWRTDEEFGREMLAGVNPVIIRRL+EFPP SKLDPK YGK
Sbjct: 361 SDGHQFLKFPVPDVIKESKTAWRTDEEFGREMLAGVNPVIIRRLQEFPPTSKLDPKLYGK 420

Query: 421 QNSSITEGHIAQHLNGLTVDQALEMNKLFILDHHDSLMPYISRINSTSTKMYATRTVLLL 480
           QNSSITE HIA+HLNGLTVDQALEMNKLFILDHHDSLMPYISRINSTSTK YATRT+L+L
Sbjct: 421 QNSSITEEHIAKHLNGLTVDQALEMNKLFILDHHDSLMPYISRINSTSTKTYATRTLLVL 480

Query: 481 QDNGILKPLAIELSLPHPQGDHHGAVSKVFTPAEHGVEGSVWQLAKAYVAVSDSGYHQLI 540
           QDNGILKP+AIELSLPHPQGDHHGAVSKVFTPAE+G+EGSVWQLAKAYVAV+DSGYHQLI
Sbjct: 481 QDNGILKPIAIELSLPHPQGDHHGAVSKVFTPAENGIEGSVWQLAKAYVAVNDSGYHQLI 540

Query: 541 SHWLNTHAVIEPFVIATNRQLSVMHPIYKLLHPHFRDTMNINAMARQVLINAGGILETTV 600
           SHWLNTHAVIEPF+IATNR LSVMHPIYKLLHPHFRDTMNINAMARQVLINAGGILE TV
Sbjct: 541 SHWLNTHAVIEPFIIATNRHLSVMHPIYKLLHPHFRDTMNINAMARQVLINAGGILEITV 600

Query: 601 FPGKYALEMSAVLYKNWIFTDQALPADLIKRGVAVPDPDSPHGLKLLIEDYPYAVDGLEI 660
           FPGKYALEMSAVLYKNW+FTDQALPADLIKRGVAVPDP S HGLKLLIEDYPYAVDGLEI
Sbjct: 601 FPGKYALEMSAVLYKNWVFTDQALPADLIKRGVAVPDPSSSHGLKLLIEDYPYAVDGLEI 660

Query: 661 WSAIETWVREYCCFYYKSDEMVEEDTEIQSWWTELRNVGHGDLKDEPWWPKMKTREELIQ 720
           WSAIETWVREY  FYYKSDEMV +DTEIQSWWTELRNVGHGDLKDEPWWPKM TRE+LIQ
Sbjct: 661 WSAIETWVREYSYFYYKSDEMVVQDTEIQSWWTELRNVGHGDLKDEPWWPKMNTREDLIQ 720

Query: 721 SCTIVIWIASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEFRELETDPDLAYLKT 780
           SC  +IWIASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEFRELETDPDLAYLKT
Sbjct: 721 SCATIIWIASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEFRELETDPDLAYLKT 780

Query: 781 ITAQLQTILGVSLIESLSRHSVDEIYLGQRETADWTSDEEAVAAFERFGDRLKEIEEKIM 840
           ITAQLQTILGVSLIESLSRHSVDEIYLGQR+TADWT+DEE +AAFERFGDRL+EIEEKI+
Sbjct: 781 ITAQLQTILGVSLIESLSRHSVDEIYLGQRDTADWTTDEEPMAAFERFGDRLREIEEKIL 840

Query: 841 RMNNDERWRNRVGPVKMPYTLLFPNTSNYYEEEGLNARGIPNSISI 885
            MNNDERWRNRVGPVKMPYTLLFPNTSN+YEEEGLNARGIPNSISI
Sbjct: 841 SMNNDERWRNRVGPVKMPYTLLFPNTSNFYEEEGLNARGIPNSISI 886

BLAST of Lcy01g002270 vs. NCBI nr
Match: XP_023514286.1 (probable linoleate 9S-lipoxygenase 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1681.8 bits (4354), Expect = 0.0e+00
Identity = 807/886 (91.08%), Postives = 847/886 (95.60%), Query Frame = 0

Query: 1   MGICPGVEMLQKMLEAFFSGNLMKFGYGGGGGGGGS--RIRGTVVLMKKCVLDFKDVKAS 60
           MGICPG+EM+QK+LEAFFS +LMKFGYGGGGGG G+  RIRGTVVLMKK +LDFKDVKAS
Sbjct: 1   MGICPGMEMIQKLLEAFFSSSLMKFGYGGGGGGSGNGRRIRGTVVLMKKSILDFKDVKAS 60

Query: 61  VLDRVHEFLGKGVSIQLISSDPSSSGDRLGAAKVGKAANLEQWILTMKPSLTADDIEFTV 120
           V+DR+HEFLGKGVSIQLISSDP +S DRLG   VGKAA LEQWI T+KPSLTAD+IEFTV
Sbjct: 61  VVDRIHEFLGKGVSIQLISSDPPASEDRLGVEMVGKAAMLEQWISTVKPSLTADEIEFTV 120

Query: 121 FFEWEERMGVPGAFIIRNHHSSQFFLKTVTLHDVPGHGSVHFVCNSWVYPAHRYTYDRVF 180
           FFEWEERMGVPGAFIIRNHHSSQF+LKTVTLHDVPG+GSVHFVCNSWVYPAHRYTYDRVF
Sbjct: 121 FFEWEERMGVPGAFIIRNHHSSQFYLKTVTLHDVPGYGSVHFVCNSWVYPAHRYTYDRVF 180

Query: 181 FSNKSYLPSETPEPLRKYREEELVNLRGNGIGELKEWDRVYDYAYYNDLGMPDMGKRFVR 240
           FSNKSYLPSETPEPLRKYRE EL+NLRGNG+GELKEWDRVYDYA YNDLGMPD  KRFVR
Sbjct: 181 FSNKSYLPSETPEPLRKYRENELMNLRGNGVGELKEWDRVYDYACYNDLGMPDTSKRFVR 240

Query: 241 TVLGGSKEFPYPRRGRTGRKPAKADPCCESRLPLLSLDIYVPRDERFRNLKFSDFLAYAV 300
           TVLGG KEFPYPRRGRTGRK AK+DP CESRLPLLSLDIYVPRDERFR+LK SDFLAYAV
Sbjct: 241 TVLGGLKEFPYPRRGRTGRKQAKSDPSCESRLPLLSLDIYVPRDERFRHLKLSDFLAYAV 300

Query: 301 KSIFQTLVPEIKSLCDKTINEFDCFQDVLDLYEGGMSLPNEILGSLRELVPWQLFRELMR 360
           KSI QTL+PEIKSLCDKTINEFDCFQDVLDLYEGGM LPNEILG+LR+LVPWQLFRELMR
Sbjct: 301 KSIVQTLLPEIKSLCDKTINEFDCFQDVLDLYEGGMRLPNEILGNLRDLVPWQLFRELMR 360

Query: 361 SDGRQFLKFPVPDVIKESKTAWRTDEEFGREMLAGVNPVIIRRLEEFPPASKLDPKAYGK 420
           SDG QFLKFPVPDVIKESKTAWRTDEEFGREMLAGVNPVIIRRL+EFPP SKLDPK YGK
Sbjct: 361 SDGHQFLKFPVPDVIKESKTAWRTDEEFGREMLAGVNPVIIRRLQEFPPTSKLDPKLYGK 420

Query: 421 QNSSITEGHIAQHLNGLTVDQALEMNKLFILDHHDSLMPYISRINSTSTKMYATRTVLLL 480
           QNSSITE HIA+HLNGLTVDQALEMNKLFILDHHDSLMPYISRINSTSTK YATRT+L+L
Sbjct: 421 QNSSITEEHIAKHLNGLTVDQALEMNKLFILDHHDSLMPYISRINSTSTKTYATRTLLVL 480

Query: 481 QDNGILKPLAIELSLPHPQGDHHGAVSKVFTPAEHGVEGSVWQLAKAYVAVSDSGYHQLI 540
           QDNGILKP+AIELSLPHPQGDHHGAVSKVFTPAE+G+EGSVWQLAKAYVAV+DSGYHQLI
Sbjct: 481 QDNGILKPIAIELSLPHPQGDHHGAVSKVFTPAENGIEGSVWQLAKAYVAVNDSGYHQLI 540

Query: 541 SHWLNTHAVIEPFVIATNRQLSVMHPIYKLLHPHFRDTMNINAMARQVLINAGGILETTV 600
           SHWLNTHAVIEPF+IATNR LSVMHPIYKLLHPHFRDTMNINAMARQVLINAGGILE TV
Sbjct: 541 SHWLNTHAVIEPFIIATNRHLSVMHPIYKLLHPHFRDTMNINAMARQVLINAGGILEITV 600

Query: 601 FPGKYALEMSAVLYKNWIFTDQALPADLIKRGVAVPDPDSPHGLKLLIEDYPYAVDGLEI 660
           FPGKYALEMSAVLYKNW+FTDQALPADLIKRGVAVPDP S HGLKLLIEDYPYAVDGLEI
Sbjct: 601 FPGKYALEMSAVLYKNWVFTDQALPADLIKRGVAVPDPSSSHGLKLLIEDYPYAVDGLEI 660

Query: 661 WSAIETWVREYCCFYYKSDEMVEEDTEIQSWWTELRNVGHGDLKDEPWWPKMKTREELIQ 720
           WSAIETWVR+Y  FYYKSDEMV +DTEIQSWWTELRNVGHGDLKDEPWWPKM TRE+LIQ
Sbjct: 661 WSAIETWVRDYSYFYYKSDEMVVQDTEIQSWWTELRNVGHGDLKDEPWWPKMNTREDLIQ 720

Query: 721 SCTIVIWIASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEFRELETDPDLAYLKT 780
           SC  +IWIASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEFRELETDPDLAYLKT
Sbjct: 721 SCATIIWIASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEFRELETDPDLAYLKT 780

Query: 781 ITAQLQTILGVSLIESLSRHSVDEIYLGQRETADWTSDEEAVAAFERFGDRLKEIEEKIM 840
           ITAQLQTILGVSLIESLSRHSVDEIYLGQR+TADWT+DEE +AAFERFGDRL+EIEEKI+
Sbjct: 781 ITAQLQTILGVSLIESLSRHSVDEIYLGQRDTADWTTDEEPMAAFERFGDRLREIEEKIL 840

Query: 841 RMNNDERWRNRVGPVKMPYTLLFPNTSNYYEEEGLNARGIPNSISI 885
            MNNDERWRNRVGPVKMPYTLLFPNTSN+YEEEGLNARGIPNSISI
Sbjct: 841 SMNNDERWRNRVGPVKMPYTLLFPNTSNFYEEEGLNARGIPNSISI 886

BLAST of Lcy01g002270 vs. NCBI nr
Match: XP_022999947.1 (probable linoleate 9S-lipoxygenase 5 [Cucurbita maxima])

HSP 1 Score: 1676.8 bits (4341), Expect = 0.0e+00
Identity = 807/886 (91.08%), Postives = 845/886 (95.37%), Query Frame = 0

Query: 1   MGICPGVEMLQKMLEAFFSGNLMKFGYGGGGGGGGS--RIRGTVVLMKKCVLDFKDVKAS 60
           MGICPG+EMLQK+LEAFFS  LMKFGYG GGGG G+  RIRGTVVLMKK VLDFKDVKAS
Sbjct: 1   MGICPGMEMLQKLLEAFFSRCLMKFGYGDGGGGSGNGRRIRGTVVLMKKSVLDFKDVKAS 60

Query: 61  VLDRVHEFLGKGVSIQLISSDPSSSGDRLGAAKVGKAANLEQWILTMKPSLTADDIEFTV 120
           V+DR+HEFLGKGVSIQLISSD  +S DRLG   VGKAA LEQWI T+KPSLTAD+IEFTV
Sbjct: 61  VVDRIHEFLGKGVSIQLISSDLPASEDRLGVEMVGKAAMLEQWISTVKPSLTADEIEFTV 120

Query: 121 FFEWEERMGVPGAFIIRNHHSSQFFLKTVTLHDVPGHGSVHFVCNSWVYPAHRYTYDRVF 180
           FFEWEERMGVPGAFIIRNHHSSQF+LKTVTLHDVPG+GSVHFVCNSWVYPAHRYTYDRVF
Sbjct: 121 FFEWEERMGVPGAFIIRNHHSSQFYLKTVTLHDVPGYGSVHFVCNSWVYPAHRYTYDRVF 180

Query: 181 FSNKSYLPSETPEPLRKYREEELVNLRGNGIGELKEWDRVYDYAYYNDLGMPDMGKRFVR 240
           FSNKSYLPS+TPEPLRKYRE EL+NLRGNG+GELKEWDRVYDYA YNDLGMPDM KRFVR
Sbjct: 181 FSNKSYLPSKTPEPLRKYRENELINLRGNGVGELKEWDRVYDYACYNDLGMPDMSKRFVR 240

Query: 241 TVLGGSKEFPYPRRGRTGRKPAKADPCCESRLPLLSLDIYVPRDERFRNLKFSDFLAYAV 300
           TVLGGSKEFPYPRRGRTGRK AK+DP CESRL LLSLDIYVPRDERF +LK SDFLAYAV
Sbjct: 241 TVLGGSKEFPYPRRGRTGRKQAKSDPSCESRLQLLSLDIYVPRDERFCHLKLSDFLAYAV 300

Query: 301 KSIFQTLVPEIKSLCDKTINEFDCFQDVLDLYEGGMSLPNEILGSLRELVPWQLFRELMR 360
           KSI QTL+PEI+SLCDKTINEFDCFQDVLDLYEGGM LPNEILG+LR+LVPWQLFRELMR
Sbjct: 301 KSIVQTLLPEIRSLCDKTINEFDCFQDVLDLYEGGMRLPNEILGNLRDLVPWQLFRELMR 360

Query: 361 SDGRQFLKFPVPDVIKESKTAWRTDEEFGREMLAGVNPVIIRRLEEFPPASKLDPKAYGK 420
           SDG QFLKFPVPDVIKESKTAWRTDEEFGREMLAGVNPVIIRRL+EFPP SKLDPK YGK
Sbjct: 361 SDGHQFLKFPVPDVIKESKTAWRTDEEFGREMLAGVNPVIIRRLQEFPPTSKLDPKLYGK 420

Query: 421 QNSSITEGHIAQHLNGLTVDQALEMNKLFILDHHDSLMPYISRINSTSTKMYATRTVLLL 480
           QNSSITE HIA+HLNGLTVDQALEMNKLFILDHHDSLMPYISRINSTSTK YATRT+L+L
Sbjct: 421 QNSSITEEHIAKHLNGLTVDQALEMNKLFILDHHDSLMPYISRINSTSTKTYATRTLLVL 480

Query: 481 QDNGILKPLAIELSLPHPQGDHHGAVSKVFTPAEHGVEGSVWQLAKAYVAVSDSGYHQLI 540
           QDNGILKP+AIELSLPHPQGDHHGAVSKVFTPAE+G+EGSVWQLAKAYVAV+DSGYHQLI
Sbjct: 481 QDNGILKPIAIELSLPHPQGDHHGAVSKVFTPAENGIEGSVWQLAKAYVAVNDSGYHQLI 540

Query: 541 SHWLNTHAVIEPFVIATNRQLSVMHPIYKLLHPHFRDTMNINAMARQVLINAGGILETTV 600
           SHWLNTHAVIEPF+IATNR LSVMHPIYKLLHPHFRDTMNINAMARQVLINAGGILE TV
Sbjct: 541 SHWLNTHAVIEPFIIATNRHLSVMHPIYKLLHPHFRDTMNINAMARQVLINAGGILEITV 600

Query: 601 FPGKYALEMSAVLYKNWIFTDQALPADLIKRGVAVPDPDSPHGLKLLIEDYPYAVDGLEI 660
           FPGKYALEMSAVLYKNW+FTDQALPADLI RGVAVPDP S HGLKLLIEDYPYAVDGLEI
Sbjct: 601 FPGKYALEMSAVLYKNWVFTDQALPADLINRGVAVPDPSSSHGLKLLIEDYPYAVDGLEI 660

Query: 661 WSAIETWVREYCCFYYKSDEMVEEDTEIQSWWTELRNVGHGDLKDEPWWPKMKTREELIQ 720
           WSAIETWVREY CFYYKSDEMV++DTEIQSWWTELRNVGHGDLKDEPWWPKM TRE+LIQ
Sbjct: 661 WSAIETWVREYSCFYYKSDEMVQQDTEIQSWWTELRNVGHGDLKDEPWWPKMNTREDLIQ 720

Query: 721 SCTIVIWIASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEFRELETDPDLAYLKT 780
           SC  +IWIASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEFRELETDPDLAYLKT
Sbjct: 721 SCATIIWIASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEFRELETDPDLAYLKT 780

Query: 781 ITAQLQTILGVSLIESLSRHSVDEIYLGQRETADWTSDEEAVAAFERFGDRLKEIEEKIM 840
           ITAQLQTILGVSLIESLSRHSVDEIYLGQR+TADWT+DEE +AAFERFGDRL+EIEEKIM
Sbjct: 781 ITAQLQTILGVSLIESLSRHSVDEIYLGQRDTADWTTDEEPMAAFERFGDRLREIEEKIM 840

Query: 841 RMNNDERWRNRVGPVKMPYTLLFPNTSNYYEEEGLNARGIPNSISI 885
            MNNDERWRNRVGPVKMPYTLLFPNTSN+YEEEGLNARGIPNSISI
Sbjct: 841 SMNNDERWRNRVGPVKMPYTLLFPNTSNFYEEEGLNARGIPNSISI 886

BLAST of Lcy01g002270 vs. NCBI nr
Match: KAG6593895.1 (putative linoleate 9S-lipoxygenase 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1673.3 bits (4332), Expect = 0.0e+00
Identity = 806/880 (91.59%), Postives = 842/880 (95.68%), Query Frame = 0

Query: 7   VEMLQKMLEAFFSGNLMKFGYGGGGGGGGS--RIRGTVVLMKKCVLDFKDVKASVLDRVH 66
           +EMLQK+LEAFFS +LMKFGYGGGGGG G+  RIRGTVVLMKK VLDFKDVKASV+DR+H
Sbjct: 1   MEMLQKLLEAFFSSSLMKFGYGGGGGGSGNGRRIRGTVVLMKKSVLDFKDVKASVVDRIH 60

Query: 67  EFLGKGVSIQLISSDPSSSGDRLGAAKVGKAANLEQWILTMKPSLTADDIEFTVFFEWEE 126
           EFLGKGVSIQLISSDP +S DRLG   VGKAA LEQWI T+KPSLTAD+IEFTVFFEWEE
Sbjct: 61  EFLGKGVSIQLISSDPPASEDRLGVEMVGKAAMLEQWISTVKPSLTADEIEFTVFFEWEE 120

Query: 127 RMGVPGAFIIRNHHSSQFFLKTVTLHDVPGHGSVHFVCNSWVYPAHRYTYDRVFFSNKSY 186
           RMGVPGAFIIRNHHSSQF+LKTVTLHDVPG+GSVHFVCNSWVYPAHRYTYDRVFFSNKSY
Sbjct: 121 RMGVPGAFIIRNHHSSQFYLKTVTLHDVPGYGSVHFVCNSWVYPAHRYTYDRVFFSNKSY 180

Query: 187 LPSETPEPLRKYREEELVNLRGNGIGELKEWDRVYDYAYYNDLGMPDMGKRFVRTVLGGS 246
           LPSETPEPLRKYRE EL+NLRGNGIGELKEWDRVYDYA YNDLGMPD  KRFVRTVLGGS
Sbjct: 181 LPSETPEPLRKYRENELINLRGNGIGELKEWDRVYDYACYNDLGMPDTSKRFVRTVLGGS 240

Query: 247 KEFPYPRRGRTGRKPAKADPCCESRLPLLSLDIYVPRDERFRNLKFSDFLAYAVKSIFQT 306
           KEFPYPRRGRTGRK AK+DP CESRLPLLSLDIYVPRDERFR+LK SDFLAYAVKSI QT
Sbjct: 241 KEFPYPRRGRTGRKQAKSDPSCESRLPLLSLDIYVPRDERFRHLKLSDFLAYAVKSIVQT 300

Query: 307 LVPEIKSLCDKTINEFDCFQDVLDLYEGGMSLPNEILGSLRELVPWQLFRELMRSDGRQF 366
           L+PEIKSLCDKTINEFDCFQDVLDLYEGGM LPNEILG+LR+LVPWQLFRELMRSDG QF
Sbjct: 301 LLPEIKSLCDKTINEFDCFQDVLDLYEGGMRLPNEILGNLRDLVPWQLFRELMRSDGHQF 360

Query: 367 LKFPVPDVIKESKTAWRTDEEFGREMLAGVNPVIIRRLEEFPPASKLDPKAYGKQNSSIT 426
           LKFPVPDVIKESKTAWRTDEEFGREMLAGVNPVIIRRL+EFPP SKLDPK YGKQNSSIT
Sbjct: 361 LKFPVPDVIKESKTAWRTDEEFGREMLAGVNPVIIRRLQEFPPTSKLDPKLYGKQNSSIT 420

Query: 427 EGHIAQHLNGLTVDQALEMNKLFILDHHDSLMPYISRINSTSTKMYATRTVLLLQDNGIL 486
           E HIA+HLNGLTVDQALEMNKLFILDHHDSLMPYISRINSTSTK YATRT+L+LQDNGIL
Sbjct: 421 EEHIAKHLNGLTVDQALEMNKLFILDHHDSLMPYISRINSTSTKTYATRTLLVLQDNGIL 480

Query: 487 KPLAIELSLPHPQGDHHGAVSKVFTPAEHGVEGSVWQLAKAYVAVSDSGYHQLISHWLNT 546
           KP+AIELSLPHPQGDHHGAVSKVFTPAE+G+EGSVWQLAKAYVAV+DSGYHQLISHWLNT
Sbjct: 481 KPIAIELSLPHPQGDHHGAVSKVFTPAENGIEGSVWQLAKAYVAVNDSGYHQLISHWLNT 540

Query: 547 HAVIEPFVIATNRQLSVMHPIYKLLHPHFRDTMNINAMARQVLINAGGILETTVFPGKYA 606
           HAVIEPF+IATNR LSVMHPIYKLLHPHFRDTMNINAMARQVLINAGGILE TVFPGKYA
Sbjct: 541 HAVIEPFIIATNRHLSVMHPIYKLLHPHFRDTMNINAMARQVLINAGGILEITVFPGKYA 600

Query: 607 LEMSAVLYKNWIFTDQALPADLIKRGVAVPDPDSPHGLKLLIEDYPYAVDGLEIWSAIET 666
           LEMSAVLYKNW+FTDQALPADLIKRGVAVPDP S HGLKLLIEDYPYAVDGLEIWSAIET
Sbjct: 601 LEMSAVLYKNWVFTDQALPADLIKRGVAVPDPSSSHGLKLLIEDYPYAVDGLEIWSAIET 660

Query: 667 WVREYCCFYYKSDEMVEEDTEIQSWWTELRNVGHGDLKDEPWWPKMKTREELIQSCTIVI 726
           WVREY  FYYKSDEMV +DTEIQSWWTELRNVGHGDLKDEPWWPKM TRE+LIQSC  +I
Sbjct: 661 WVREYSYFYYKSDEMVVQDTEIQSWWTELRNVGHGDLKDEPWWPKMNTREDLIQSCATII 720

Query: 727 WIASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEFRELETDPDLAYLKTITAQLQ 786
           WIASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEFRELETDPDLAYLKTITAQLQ
Sbjct: 721 WIASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEFRELETDPDLAYLKTITAQLQ 780

Query: 787 TILGVSLIESLSRHSVDEIYLGQRETADWTSDEEAVAAFERFGDRLKEIEEKIMRMNNDE 846
           TILGVSLIESLSRHSVDEIYLGQR+TADWT+DEE +AAFERFGDRL+EIEEKI+ MNNDE
Sbjct: 781 TILGVSLIESLSRHSVDEIYLGQRDTADWTTDEEPMAAFERFGDRLREIEEKILSMNNDE 840

Query: 847 RWRNRVGPVKMPYTLLFPNTSNYYEEEGLNARGIPNSISI 885
           RWRNRVGPVKMPYTLLFPNTSN+YEEEGLNARGIPNSISI
Sbjct: 841 RWRNRVGPVKMPYTLLFPNTSNFYEEEGLNARGIPNSISI 880

BLAST of Lcy01g002270 vs. NCBI nr
Match: XP_038874760.1 (probable linoleate 9S-lipoxygenase 5 [Benincasa hispida])

HSP 1 Score: 1669.4 bits (4322), Expect = 0.0e+00
Identity = 801/884 (90.61%), Postives = 839/884 (94.91%), Query Frame = 0

Query: 1   MGICPGVEMLQKMLEAFFSGNLMKFGYGGGGGGGGSRIRGTVVLMKKCVLDFKDVKASVL 60
           MGICPG+EMLQK+LEAFFSG L+KFGYGGGG G   RIRGTVV+MKKCVLDFKDVKAS+L
Sbjct: 1   MGICPGMEMLQKLLEAFFSGILLKFGYGGGGDGDQKRIRGTVVIMKKCVLDFKDVKASIL 60

Query: 61  DRVHEFLGKGVSIQLISSDPSSSGDRLGAAKVGKAANLEQWILTMKPSLTADDIEFTVFF 120
           DRVHEF GKGV+IQLISS+P  S D L  A VGKAANLEQWI T+KPSLTAD+IE TV F
Sbjct: 61  DRVHEFFGKGVTIQLISSNPPQS-DGLETAIVGKAANLEQWISTVKPSLTADEIELTVLF 120

Query: 121 EWEERMGVPGAFIIRNHHSSQFFLKTVTLHDVPGHGSVHFVCNSWVYPAHRYTYDRVFFS 180
           EWEERMGVPGAFIIRNHH +QF+LKTVTLHDVPG+GSVHFVCNSWVYPAHRYTYDRVFFS
Sbjct: 121 EWEERMGVPGAFIIRNHHPNQFYLKTVTLHDVPGYGSVHFVCNSWVYPAHRYTYDRVFFS 180

Query: 181 NKSYLPSETPEPLRKYREEELVNLRGNGIGELKEWDRVYDYAYYNDLGMPDMGKRFVRTV 240
           NKSYLP+ETPE LRKYREEELVNLRGNG GELKEWDRVYDYA YNDLGMP+ GK++VRT+
Sbjct: 181 NKSYLPNETPESLRKYREEELVNLRGNGNGELKEWDRVYDYACYNDLGMPNSGKKYVRTI 240

Query: 241 LGGSKEFPYPRRGRTGRKPAKADPCCESRLPLLSLDIYVPRDERFRNLKFSDFLAYAVKS 300
           LGGSKEFPYPRRGRT RKPAKADP CE RLPLLSLDIYVPRDERFRNLK SDFLAY VKS
Sbjct: 241 LGGSKEFPYPRRGRTSRKPAKADPSCECRLPLLSLDIYVPRDERFRNLKLSDFLAYGVKS 300

Query: 301 IFQTLVPEIKSLCDKTINEFDCFQDVLDLYEGGMSLPNEILGSLRELVPWQLFRELMRSD 360
           I QTL+PEIKSLCDKTINEFD FQDVLDLYEGGM+LP E+LG+LRELVPWQLFRELMRSD
Sbjct: 301 IVQTLLPEIKSLCDKTINEFDSFQDVLDLYEGGMTLPTEVLGTLRELVPWQLFRELMRSD 360

Query: 361 GRQFLKFPVPDVIKESKTAWRTDEEFGREMLAGVNPVIIRRLEEFPPASKLDPKAYGKQN 420
           GRQFLKFPVPDVIKESKTAWRTDEEFGREMLAGVNPVIIRRLEEFPPASKLDPK YG QN
Sbjct: 361 GRQFLKFPVPDVIKESKTAWRTDEEFGREMLAGVNPVIIRRLEEFPPASKLDPKTYGNQN 420

Query: 421 SSITEGHIAQHLNGLTVDQALEMNKLFILDHHDSLMPYISRINSTSTKMYATRTVLLLQD 480
           SSIT  HIA+HLNGLT+DQALEMNKLFILDHHDSLMPYISRINSTSTK YATRT+LLLQD
Sbjct: 421 SSITAEHIAEHLNGLTIDQALEMNKLFILDHHDSLMPYISRINSTSTKTYATRTLLLLQD 480

Query: 481 NGILKPLAIELSLPHPQGDHHGAVSKVFTPAEHGVEGSVWQLAKAYVAVSDSGYHQLISH 540
           NGILKPLAIELSLPHPQG HHGAVSK+FTPAEHGVEGSVWQLAKAYVAV+DSGYHQLISH
Sbjct: 481 NGILKPLAIELSLPHPQGYHHGAVSKIFTPAEHGVEGSVWQLAKAYVAVNDSGYHQLISH 540

Query: 541 WLNTHAVIEPFVIATNRQLSVMHPIYKLLHPHFRDTMNINAMARQVLINAGGILETTVFP 600
           WLNTHAVIEPF+IATNRQLSV+HPIYKLLHPHFRDTMNINAMARQVLINAGGILETTVFP
Sbjct: 541 WLNTHAVIEPFIIATNRQLSVVHPIYKLLHPHFRDTMNINAMARQVLINAGGILETTVFP 600

Query: 601 GKYALEMSAVLYKNWIFTDQALPADLIKRGVAVPDPDSPHGLKLLIEDYPYAVDGLEIWS 660
           GKYALEMSAVLYKNW+FTDQALP DLIKRGVA+PDP SPHGLKLLIEDYPYAVDGLEIWS
Sbjct: 601 GKYALEMSAVLYKNWVFTDQALPTDLIKRGVAIPDPSSPHGLKLLIEDYPYAVDGLEIWS 660

Query: 661 AIETWVREYCCFYYKSDEMVEEDTEIQSWWTELRNVGHGDLKDEPWWPKMKTREELIQSC 720
           AIE WVR+Y  FYYKSDEMVEEDTEIQSWWTELR VGHGDLKDEPWWPKM TRE+LIQSC
Sbjct: 661 AIEKWVRDYSSFYYKSDEMVEEDTEIQSWWTELRTVGHGDLKDEPWWPKMNTREDLIQSC 720

Query: 721 TIVIWIASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEFRELETDPDLAYLKTIT 780
           TI+IWIASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEF+ELETDPDLAYLKTIT
Sbjct: 721 TIIIWIASALHAAVNFGQYPYAGYLPNRPTVSRRFMPEPGTPEFKELETDPDLAYLKTIT 780

Query: 781 AQLQTILGVSLIESLSRHSVDEIYLGQRETADWTSDEEAVAAFERFGDRLKEIEEKIMRM 840
           AQLQTILGVSLIESLSRHSVDEIYLGQR+TADWT DEEAVAAFERFGDRL+EIEEKIMRM
Sbjct: 781 AQLQTILGVSLIESLSRHSVDEIYLGQRDTADWTKDEEAVAAFERFGDRLREIEEKIMRM 840

Query: 841 NNDERWRNRVGPVKMPYTLLFPNTSNYYEEEGLNARGIPNSISI 885
           NNDE+WRNRVGPVKMPYT LFPNTSN+YEEEGLN RGIPNSISI
Sbjct: 841 NNDEKWRNRVGPVKMPYTFLFPNTSNHYEEEGLNVRGIPNSISI 883

BLAST of Lcy01g002270 vs. TAIR 10
Match: AT3G22400.1 (PLAT/LH2 domain-containing lipoxygenase family protein )

HSP 1 Score: 1236.9 bits (3199), Expect = 0.0e+00
Identity = 580/855 (67.84%), Postives = 714/855 (83.51%), Query Frame = 0

Query: 37  RIRGTVVLMKKCVLDFKDVKASVLDRVHEFLGKGVSIQLISS-DPSSSGDRLGAAKVGKA 96
           +I G VV+MKK +LDFKDV AS+LDRV+E LG+ VS+ LISS  P  + ++ G  ++GKA
Sbjct: 34  KIEGEVVVMKKNLLDFKDVMASLLDRVNELLGRRVSLHLISSHQPDPANEKRG--RLGKA 93

Query: 97  ANLEQWILTMKPSLTADDIEFTVFFEWEERMGVPGAFIIRNHHSSQFFLKTVTLHDVP-- 156
           A+LE+W+  +K S+TA++  F V F+W+E MG P AF+I+NHH SQF+LK++TL   P  
Sbjct: 94  AHLEKWVTKIKTSVTAEETAFGVTFDWDESMGPPAAFVIKNHHHSQFYLKSLTLRGFPDG 153

Query: 157 --GHGSVHFVCNSWVYPAHRYTYDRVFFSNKSYLPSETPEPLRKYREEELVNLRGNGI-G 216
             G  ++HF+CNSW+YP HRY  DRVFFSNK+YLPSETPE +++ REEEL NLRGN   G
Sbjct: 154 EGGATAIHFICNSWIYPNHRYRSDRVFFSNKAYLPSETPELIKELREEELKNLRGNEKGG 213

Query: 217 ELKEWDRVYDYAYYNDLGMPDMGKRFVRTVLGGSKEFPYPRRGRTGRKPAKADPCCESRL 276
           E KEWDRVYDYAYYNDLG PD G   VR VLGGS E PYPRRG+TGRK  K+DP  ESRL
Sbjct: 214 EFKEWDRVYDYAYYNDLGAPDKGPDSVRPVLGGSPELPYPRRGKTGRKSTKSDPKSESRL 273

Query: 277 PLLSLDIYVPRDERFRNLKFSDFLAYAVKSIFQTLVPEIKSLCDKTINEFDCFQDVLDLY 336
            LL+L+IYVPRDERF ++KFSDFLAYA+KS+ Q LVPEI S+CDKTINEFD F+DV  LY
Sbjct: 274 ALLNLNIYVPRDERFSHVKFSDFLAYALKSVTQVLVPEIASVCDKTINEFDSFEDVFHLY 333

Query: 337 EGGMSLPN-EILGSLRELVPWQLFRELMRSDGRQFLKFPVPDVIKESKTAWRTDEEFGRE 396
           +G + L N   +  LR+++PW++FREL+R+DG +FLK+P+PD++KES++AWRTDEEF RE
Sbjct: 334 DGSIKLANGHTISKLRDVIPWEMFRELVRNDGERFLKYPLPDILKESRSAWRTDEEFARE 393

Query: 397 MLAGVNPVIIRRLEEFPPASKLDPKAYGKQNSSITEGHIAQHLNGLTVDQALEMNKLFIL 456
           MLAG+NPV+I RL+EFPP S LD   YG Q+SSI   HI  ++NGL V +ALE NKL+IL
Sbjct: 394 MLAGLNPVVISRLQEFPPKSCLDSAKYGNQHSSIRTEHIESNMNGLNVQEALEQNKLYIL 453

Query: 457 DHHDSLMPYISRINSTSTKMYATRTVLLLQDNGILKPLAIELSLPHPQGDHHGAVSKVFT 516
           DHHD+LMPY++RINST+TK YATRT+LLLQ +G LKPLAIELSLPH QG+ +G+VSKVFT
Sbjct: 454 DHHDALMPYLTRINSTNTKTYATRTLLLLQADGTLKPLAIELSLPHAQGESYGSVSKVFT 513

Query: 517 PAEHGVEGSVWQLAKAYVAVSDSGYHQLISHWLNTHAVIEPFVIATNRQLSVMHPIYKLL 576
           PAE GVEGSVWQLAKAY AV+DSGYHQLISHWL THAVIEPF+IA+NRQLSV+HPI+KLL
Sbjct: 514 PAEKGVEGSVWQLAKAYAAVNDSGYHQLISHWLQTHAVIEPFIIASNRQLSVVHPIHKLL 573

Query: 577 HPHFRDTMNINAMARQVLINAGGILETTVFPGKYALEMSAVLYKNWIFTDQALPADLIKR 636
           HPHFRDTMNINA+AR VLIN+ G+LE TVFP +YA+EMS+ +YKNW+FT+QALP DL+KR
Sbjct: 574 HPHFRDTMNINALARHVLINSDGVLERTVFPSRYAMEMSSSIYKNWVFTEQALPKDLLKR 633

Query: 637 GVAVPDPDSPHGLKLLIEDYPYAVDGLEIWSAIETWVREYCCFYYKSDEMVEEDTEIQSW 696
           GVAV DP+S +G+KLLIEDYP+AVDGLEIWSAI+TWV EYC FYY +D+ V+ DTEIQSW
Sbjct: 634 GVAVEDPNSDNGVKLLIEDYPFAVDGLEIWSAIKTWVTEYCTFYYNNDKTVQTDTEIQSW 693

Query: 697 WTELRNVGHGDLKDEPWWPKMKTREELIQSCTIVIWIASALHAAVNFGQYPYAGYLPNRP 756
           WTELR  GHGD + E WWP M+TR++LI++CTI+IWIASALHAAVNFGQYPYAG+LPNRP
Sbjct: 694 WTELRTKGHGDKRHESWWPSMQTRDDLIETCTIIIWIASALHAAVNFGQYPYAGFLPNRP 753

Query: 757 TVSRRFMPEPGTPEFRELETDPDLAYLKTITAQLQTILGVSLIESLSRHSVDEIYLGQRE 816
           TVSRRFMPEPGT E+ ELE D D+A+LKTIT QLQT+LG+S+IE LS HS DEIYLGQR+
Sbjct: 754 TVSRRFMPEPGTDEYAELEEDADVAFLKTITPQLQTLLGISIIEILSMHSTDEIYLGQRD 813

Query: 817 TADWTSDEEAVAAFERFGDRLKEIEEKIMRMNNDERWRNRVGPVKMPYTLLFPNTSNYYE 876
           + +WT+D+E + AF+RFG  L+ IE  I+R NND+R++NR GPV +PYTLL+PNT++Y  
Sbjct: 814 SPNWTADDEPLEAFKRFGKELELIENNIIRRNNDKRFKNRTGPVNIPYTLLYPNTTDYTR 873

Query: 877 EEGLNARGIPNSISI 885
           E G+  +GIPNS+SI
Sbjct: 874 EGGITGKGIPNSVSI 886

BLAST of Lcy01g002270 vs. TAIR 10
Match: AT1G55020.1 (lipoxygenase 1 )

HSP 1 Score: 1168.3 bits (3021), Expect = 0.0e+00
Identity = 559/868 (64.40%), Postives = 698/868 (80.41%), Query Frame = 0

Query: 20  GNLMKFGYGGGGGGGGSRIRGTVVLMKKCVLDFKDVKASVLDRVHEFLGKGVSIQLISSD 79
           G L     GGG      +++GTVVLMKK VLDF D  AS LDR+HEFLG  ++++L+SSD
Sbjct: 3   GELRDLLTGGGNETTTKKVKGTVVLMKKNVLDFNDFNASFLDRLHEFLGNKITLRLVSSD 62

Query: 80  PSSSGDRLGAAKVGKAANLEQWILTMKPSLTADDIEFTVFFEWEERMGVPGAFIIRNHHS 139
            + S +     K+GKAA+LE WI T+  SLTA +  F V F++E   G PGAF+IRN H 
Sbjct: 63  VTDS-ENGSKGKLGKAAHLEDWITTI-TSLTAGESAFKVTFDYETDFGYPGAFLIRNSHF 122

Query: 140 SQFFLKTVTLHDVPGHGSVHFVCNSWVYPAHRYTYDRVFFSNKSYLPSETPEPLRKYREE 199
           S+F LK++TL DVPGHG VH++CNSW+YPA  YT DRVFFSNK+YLP ETP  L KYREE
Sbjct: 123 SEFLLKSLTLEDVPGHGRVHYICNSWIYPAKHYTTDRVFFSNKTYLPHETPATLLKYREE 182

Query: 200 ELVNLRGNGIGELKEWDRVYDYAYYNDLGMPDMGKRFVRTVLGGSKEFPYPRRGRTGRKP 259
           ELV+LRG G GELKEWDRVYDYAYYNDLG+P       R VLGG++E+PYPRRGRTGRKP
Sbjct: 183 ELVSLRGTGEGELKEWDRVYDYAYYNDLGVPPKNP---RPVLGGTQEYPYPRRGRTGRKP 242

Query: 260 AKADPCCESRLPLL-SLDIYVPRDERFRNLKFSDFLAYAVKSIFQTLVPEIKSLCDKTIN 319
            K DP  ESRLP+  SLDIYVPRDERF +LK SDFLAYA+K+I Q + P ++++ D T  
Sbjct: 243 TKEDPQTESRLPITSSLDIYVPRDERFGHLKMSDFLAYALKAIAQFIQPALEAVFDDTPK 302

Query: 320 EFDCFQDVLDLYEGGMSLPNE-ILGSLRELVPWQLFRELMRSDGRQFLKFPVPDVIKESK 379
           EFD F+DVL +YE G+ LPN+ ++ S+ + +P ++ +E+ R+DG++FLKFPVP VIKE K
Sbjct: 303 EFDSFEDVLKIYEEGIDLPNQALIDSIVKNIPLEMLKEIFRTDGQKFLKFPVPQVIKEDK 362

Query: 380 TAWRTDEEFGREMLAGVNPVIIRRLEEFPPASKLDPKAYGKQNSSITEGHIAQHLNGLTV 439
           TAWRTDEEF REMLAG+NPV+I+ L+EFPP SKLD ++YG QNS+IT+ HI  +L+GLTV
Sbjct: 363 TAWRTDEEFAREMLAGLNPVVIQLLKEFPPKSKLDSESYGNQNSTITKSHIEHNLDGLTV 422

Query: 440 DQALEMNKLFILDHHDSLMPYISRINSTSTKMYATRTVLLLQDNGILKPLAIELSLPHPQ 499
           ++ALE  +LFILDHHD+LMPY+ R+N+T+TK YA+RT+L L+D+G LKPL IELSLPHP 
Sbjct: 423 EEALEKERLFILDHHDTLMPYLGRVNTTTTKTYASRTLLFLKDDGTLKPLVIELSLPHPN 482

Query: 500 GDHHGAVSKVFTPAEHGVEGSVWQLAKAYVAVSDSGYHQLISHWLNTHAVIEPFVIATNR 559
           GD  GAVS+V+TP E GV  S+WQLAKA+V V+DSG HQLISHW+ THA IEPFVIATNR
Sbjct: 483 GDKFGAVSEVYTPGE-GVYDSLWQLAKAFVGVNDSGNHQLISHWMQTHASIEPFVIATNR 542

Query: 560 QLSVMHPIYKLLHPHFRDTMNINAMARQVLINAGGILETTVFPGKYALEMSAVLYKN-WI 619
           QLSV+HP++KLL PHFRDTMNINA+ARQ+LIN GGI E TVFP KYA+EMS+ +YKN W 
Sbjct: 543 QLSVLHPVFKLLEPHFRDTMNINALARQILINGGGIFEITVFPSKYAMEMSSFIYKNHWT 602

Query: 620 FTDQALPADLIKRGVAVPDPDSPHGLKLLIEDYPYAVDGLEIWSAIETWVREYCCFYYKS 679
           F DQALPA+L KRG+AV DP++PHGL+L I+DYPYAVDGLE+W AIE+WVR+Y   +YK 
Sbjct: 603 FPDQALPAELKKRGMAVEDPEAPHGLRLRIKDYPYAVDGLEVWYAIESWVRDYIFLFYKI 662

Query: 680 DEMVEEDTEIQSWWTELRNVGHGDLKDEPWWPKMKTREELIQSCTIVIWIASALHAAVNF 739
           +E ++ DTE+Q+WW E+R  GHGD K EPWWPKM+TREEL++SCTI+IW+ASALHAAVNF
Sbjct: 663 EEDIQTDTELQAWWKEVREEGHGDKKSEPWWPKMQTREELVESCTIIIWVASALHAAVNF 722

Query: 740 GQYPYAGYLPNRPTVSRRFMPEPGTPEFRELETDPDLAYLKTITAQLQTILGVSLIESLS 799
           GQYP AGYLPNRPT+SR++MP+  TPEF ELE +PD  +LKTITAQLQT+LG+SLIE LS
Sbjct: 723 GQYPVAGYLPNRPTISRQYMPKENTPEFEELEKNPDKVFLKTITAQLQTLLGISLIEILS 782

Query: 800 RHSVDEIYLGQRETADWTSDEEAVAAFERFGDRLKEIEEKIMRMNNDERWRNRVGPVKMP 859
            HS DE+YLGQR++ +W +++EA+ AFE+FG+++KEIE+ I   N+DE  +NR G VKMP
Sbjct: 783 THSSDEVYLGQRDSKEWAAEKEALEAFEKFGEKVKEIEKNIDERNDDETLKNRTGLVKMP 842

Query: 860 YTLLFPNTSNYYEEEGLNARGIPNSISI 885
           YTLLFP++     E G+  RGIPNS+SI
Sbjct: 843 YTLLFPSS-----EGGVTGRGIPNSVSI 859

BLAST of Lcy01g002270 vs. TAIR 10
Match: AT1G72520.1 (PLAT/LH2 domain-containing lipoxygenase family protein )

HSP 1 Score: 740.0 bits (1909), Expect = 2.3e-213
Identity = 378/854 (44.26%), Postives = 530/854 (62.06%), Query Frame = 0

Query: 37  RIRGTVVLMKKCVLDFKDVKASVLDRVHEFLGKGVSIQLISS--DPSSSGDRLGAAKVGK 96
           ++R    +  K   DFK+     LD   + +G+ V ++L+S+  DP ++       K  K
Sbjct: 91  KVRAVATVRNKNKEDFKETLVKHLDAFTDKIGRNVVLELMSTQVDPKTN-----EPKKSK 150

Query: 97  AANLEQWILTMKPSLTADDIEFTVFFEWEERMGVPGAFIIRNHHSSQFFLKTVTLHDVPG 156
           AA L+ W  + K +  A+ + +T  F  +   G PGA  + N H  +FFL+++T+     
Sbjct: 151 AAVLKDW--SKKSNSKAERVHYTAEFTVDSAFGSPGAITVTNKHQKEFFLESITIEGF-A 210

Query: 157 HGSVHFVCNSWVYPAHRYTYDRVFFSNKSYLPSETPEPLRKYREEELVNLRGNGIGELKE 216
            G VHF CNSWV     +   R+ F+N+ YLPSETP  LR  RE+EL NLRGNG GE K 
Sbjct: 211 CGPVHFPCNSWVQSQKDHPSKRILFTNQPYLPSETPSGLRTLREKELENLRGNGKGERKL 270

Query: 217 WDRVYDYAYYNDLGMPDMGKRFVRTVLGGSKEFPYPRRGRTGRKPAKADPCCESRLPLLS 276
            DR+YDY  YND+G PD+ +   R  LGG +EFPYPRR RTGR     D   E R+    
Sbjct: 271 SDRIYDYDVYNDIGNPDISRELARPTLGG-REFPYPRRCRTGRSSTDTDMMSERRVE-KP 330

Query: 277 LDIYVPRDERFRNLKFSDFLAYAVKSIFQTLVPEIKSLCDKTINEFDCFQDVLDLYEGGM 336
           L +YVPRDE+F   K + F A  +K++   L+P +K+       +F  F ++  LY+ G+
Sbjct: 331 LPMYVPRDEQFEESKQNTFAACRLKAVLHNLIPSLKA--SILAEDFANFGEIDSLYKEGL 390

Query: 337 SLPNEILGSLRELVPWQLFRELMRSDGRQFLKFPVPDVIKESKTAWRTDEEFGREMLAGV 396
            L       + +  P       ++      L++  P ++ + K AW  D+EF R+ +AG+
Sbjct: 391 LLKLGFQDDMFKKFPLPKIVTTLQKSSEGLLRYDTPKIVSKDKYAWLRDDEFARQAIAGI 450

Query: 397 NPVIIRRLEEFPPASKLDPKAYGK-QNSSITEGHIAQHLNGLTVDQALEMNKLFILDHHD 456
           NPV I R+  +PP S LDP+ YG   +S++TE HI   L+GLTV QALE N+LF++D+HD
Sbjct: 451 NPVNIERVTSYPPVSNLDPEIYGPGLHSALTEDHIIGQLDGLTVQQALETNRLFMVDYHD 510

Query: 457 SLMPYISRINS-TSTKMYATRTVLLLQDNGILKPLAIELSLPHPQGDHHGAVSKVFTPAE 516
             +P++ RIN+    K YATRT+L L   G LKP+AIELSLP  Q   +    +V TP  
Sbjct: 511 IYLPFLDRINALDGRKAYATRTILFLTRLGTLKPIAIELSLP-SQSSSNQKSKRVVTPPV 570

Query: 517 HGVEGSVWQLAKAYVAVSDSGYHQLISHWLNTHAVIEPFVIATNRQLSVMHPIYKLLHPH 576
                 +WQLAKA+V  +D+G HQL++HWL THA +EPF++A +RQLS MHPI+KLL PH
Sbjct: 571 DATSNWMWQLAKAHVGSNDAGVHQLVNHWLRTHACLEPFILAAHRQLSAMHPIFKLLDPH 630

Query: 577 FRDTMNINAMARQVLINAGGILETTVFPGKYALEMSAVLYKN-WIFTDQALPADLIKRGV 636
            R T+ INA+ARQ LI+A G++E+    G+Y LE+S+  YKN W F  + LPADLI+RG+
Sbjct: 631 MRYTLEINAVARQTLISADGVIESCFTAGQYGLEISSAAYKNKWRFDMEGLPADLIRRGM 690

Query: 637 AVPDPDSPHGLKLLIEDYPYAVDGLEIWSAIETWVREYCCFYYKSDEMVEEDTEIQSWWT 696
           AVPDP  PHGLKLL+EDYPYA DGL +WSAI+TWVR Y   YY +  +++ DTE+Q+W++
Sbjct: 691 AVPDPTQPHGLKLLVEDYPYANDGLLLWSAIQTWVRTYVERYYANSNLIQTDTELQAWYS 750

Query: 697 ELRNVGHGDLKDEPWWPKMKTREELIQSCTIVIWIASALHAAVNFGQYPYAGYLPNRPTV 756
           E  NVGH D +D  WWPK+ T E+L+   T +IW+ASA HAA+NFGQYPY GY+PNRP +
Sbjct: 751 ESINVGHADHRDAEWWPKLSTVEDLVSVITTIIWLASAQHAALNFGQYPYGGYVPNRPPL 810

Query: 757 SRRFMPEPGTPEFRELETDPDLAYLKTITAQLQTILGVSLIESLSRHSVDEIYLGQRETA 816
            RR +P+   PEF     DP   +  ++ + LQT   ++++++LS HS DE Y+G+R+  
Sbjct: 811 MRRLIPDESDPEFTSFIEDPQKYFFSSMPSLLQTTKFMAVVDTLSTHSPDEEYIGERQQP 870

Query: 817 D-WTSDEEAVAAFERFGDRLKEIEEKIMRMNNDERWRNRVGPVKMPYTLLFPNTSNYYEE 876
             WT D E V AF  F   +  IE++I + N D   RNR G   +PY L+ P++     E
Sbjct: 871 SIWTGDAEIVDAFYGFSAEIGRIEKEIDKRNRDPSRRNRCGAGVLPYELMAPSS-----E 926

Query: 877 EGLNARGIPNSISI 885
            G+  RG+PNS+SI
Sbjct: 931 PGVTCRGVPNSVSI 926

BLAST of Lcy01g002270 vs. TAIR 10
Match: AT1G17420.1 (lipoxygenase 3 )

HSP 1 Score: 728.8 bits (1880), Expect = 5.3e-210
Identity = 376/854 (44.03%), Postives = 532/854 (62.30%), Query Frame = 0

Query: 37  RIRGTVVLMKKCVLDFKDVKASVLDRVHEFLGKGVSIQLISS--DPSSSGDRLGAAKVGK 96
           ++R  V +  K   D K+     LD   + +G+ + ++LIS+  DP +        K   
Sbjct: 85  KVRAVVTVRNKNKEDLKETLVKHLDAFADKIGRNIVLELISTQLDPKTK-----LPKKSN 144

Query: 97  AANLEQWILTMKPSLTADDIEFTVFFEWEERMGVPGAFIIRNHHSSQFFLKTVTLHDVPG 156
           AA L+ W  + K    A+ + +T  F  +   G PGA  + N H  +FFL+++T+     
Sbjct: 145 AAVLKDW--SKKSKTKAERVHYTAEFTVDAAFGSPGAITVMNKHQKEFFLESITIEGF-A 204

Query: 157 HGSVHFVCNSWVYPAHRYTYDRVFFSNKSYLPSETPEPLRKYREEELVNLRGNGIGELKE 216
            G VHF CNSWV     +   R+FF+N+ YLP+ETP  LR  RE+EL NLRG+G G  K 
Sbjct: 205 LGPVHFPCNSWVQSQKDHPDKRIFFTNQPYLPNETPSGLRVLREKELKNLRGDGSGVRKL 264

Query: 217 WDRVYDYAYYNDLGMPDMGKRFVRTVLGGSKEFPYPRRGRTGRKPAKADPCCESRLPLLS 276
            DR+YD+  YNDLG PD      R  LGG KE PYPRR RTGR+   +D   ESR+    
Sbjct: 265 SDRIYDFDVYNDLGNPDKSSELSRPKLGG-KEVPYPRRCRTGRQSTVSDKDAESRVE-KP 324

Query: 277 LDIYVPRDERFRNLKFSDFLAYAVKSIFQTLVPEIKSLCDKTINEFDCFQDVLDLYEGGM 336
           L +YVPRDE+F   K   F A  +K++   L+P +K+       +F  F ++  LY+ G+
Sbjct: 325 LPMYVPRDEQFEESKQDTFAAGRLKAVLHHLIPSLKA--SIVAEDFADFGEIDRLYKEGL 384

Query: 337 SLPNEILGSLRELVPW-QLFRELMRSDGRQFLKFPVPDVIKESKTAWRTDEEFGREMLAG 396
            L       + +  P  ++  + ++   +  LK+  P ++ + K AW  D+EF R+ +AG
Sbjct: 385 LLKLGFQDDIFKKFPLPKVVVDTLQESTKGLLKYDTPKILSKDKNAWLRDDEFARQAIAG 444

Query: 397 VNPVIIRRLEEFPPASKLDPKAYGKQNSSITEGHIAQHLNGLTVDQALEMNKLFILDHHD 456
           +NPV I R++ FPP S LDPK YG Q+S++T+ HI  HL+G +V QALE N+L++LD+HD
Sbjct: 445 INPVNIERVKTFPPVSNLDPKIYGPQHSALTDDHIIGHLDGFSVQQALEENRLYMLDYHD 504

Query: 457 SLMPYISRINS-TSTKMYATRTVLLLQDNGILKPLAIELSLPHPQGDHHGAVSKVFTPAE 516
             +P++ RIN+    K YATRT+  L   G LKP+AIELSLP P G  H +  +V TP  
Sbjct: 505 IFLPFLDRINALDGRKAYATRTIFFLTRLGTLKPVAIELSLP-PHGPKHRS-KRVLTPPV 564

Query: 517 HGVEGSVWQLAKAYVAVSDSGYHQLISHWLNTHAVIEPFVIATNRQLSVMHPIYKLLHPH 576
                 +WQLAKA+V+ +D+G HQL++HWL THA +EPF++A +RQLS MHPI+KLL PH
Sbjct: 565 DATSNWMWQLAKAHVSSNDAGVHQLVNHWLRTHACLEPFILAAHRQLSAMHPIFKLLDPH 624

Query: 577 FRDTMNINAMARQVLINAGGILETTVFPGKYALEMSAVLYK-NWIFTDQALPADLIKRGV 636
            R T+ INA+ARQ LI+A G++E     G Y +EMSA  YK +W F  + LPADLI+RG+
Sbjct: 625 MRYTLEINALARQSLISADGVIEGGFTAGAYGMEMSAAAYKSSWRFDMEGLPADLIRRGM 684

Query: 637 AVPDPDSPHGLKLLIEDYPYAVDGLEIWSAIETWVREYCCFYYKSDEMVEEDTEIQSWWT 696
           A+PD   PHGLKLLIEDYPYA DGL +WSAI+TWVR Y   YY +  +++ D+E+QSW++
Sbjct: 685 AIPDATQPHGLKLLIEDYPYANDGLLLWSAIQTWVRTYVERYYPNPNLIKTDSELQSWYS 744

Query: 697 ELRNVGHGDLKDEPWWPKMKTREELIQSCTIVIWIASALHAAVNFGQYPYAGYLPNRPTV 756
           E  NVGH DL+D  WWP++ T ++L+   T +IW+ASA HAA+NFGQYPY GY+PNRP +
Sbjct: 745 ESINVGHADLRDADWWPELSTVDDLVSILTTLIWLASAQHAALNFGQYPYGGYVPNRPPL 804

Query: 757 SRRFMPEPGTPEFRELETDPDLAYLKTITAQLQTILGVSLIESLSRHSVDEIYLGQRETA 816
            RR +P+   PE+    + P+  Y  ++ +  QT   ++++++LS HS DE Y+G+R+  
Sbjct: 805 MRRLIPDESDPEYASFISHPEKYYFSSMPSLAQTSKFMAVVDTLSTHSPDEEYIGERQQP 864

Query: 817 D-WTSDEEAVAAFERFGDRLKEIEEKIMRMNNDERWRNRVGPVKMPYTLLFPNTSNYYEE 876
             WT D E V AF  F   +  IE++I + N D   RNR G   +PY LL P++     E
Sbjct: 865 SIWTGDAEIVEAFYGFAAEIGRIEKEIEKRNADPDRRNRCGAGVLPYELLVPSS-----E 919

Query: 877 EGLNARGIPNSISI 885
            G+  RG+PNS+SI
Sbjct: 925 PGVTCRGVPNSVSI 919

BLAST of Lcy01g002270 vs. TAIR 10
Match: AT3G45140.1 (lipoxygenase 2 )

HSP 1 Score: 673.7 bits (1737), Expect = 2.0e-193
Identity = 361/833 (43.34%), Postives = 502/833 (60.26%), Query Frame = 0

Query: 60  LDRVHEFLGKGVSIQLISSDPSSSGDRLGAAKVGKAANLEQWILTMKPSLTADDIEFTVF 119
           LD + +  G+ + ++LIS            AK  +   +E +    +    A D ++   
Sbjct: 95  LDDIADIRGRSLLVELIS------------AKTDQRITVEDY--AQRVWAEAPDEKYECE 154

Query: 120 FEWEERMGVPGAFIIRNHHSSQFFLKTVTLHDVPGHGSVHFVCNSWVYPAHRYTYDRVFF 179
           FE  E  G  GA  I+N +  Q FLK V L  +PG GS+ F C SWV P       R+FF
Sbjct: 155 FEMPEDFGPVGAIKIQNQYHRQLFLKGVEL-KLPG-GSITFTCESWVAPKSVDPTKRIFF 214

Query: 180 SNKSYLPSETPEPLRKYREEELVNLRGNG---IGELKEWDRVYDYAYYNDLGMPDMGKRF 239
           S+KSYLPS+TPEPL+KYR+EEL  L+G     +GE  +++R+YDY  YND+G PD     
Sbjct: 215 SDKSYLPSQTPEPLKKYRKEELETLQGKNREEVGEFTKFERIYDYDVYNDVGDPDNDPEL 274

Query: 240 VRTVLGGSKEFPYPRRGRTGRKPAKADPCCESRLPLLSLDIYVPRDERFRNLKFSDFLAY 299
            R V+GG    PYPRR +TGRKP + DP  E R      + YVPRDE F   K + F   
Sbjct: 275 ARPVIGGLTH-PYPRRCKTGRKPCETDPSSEQR---YGGEFYVPRDEEFSTAKGTSFTGK 334

Query: 300 AVKSIFQTLVPEIKSLCDKTINEFDCFQDVLDLYEGGMSLPNEILGSLRELVPWQLFREL 359
           AV +   ++ P+I+S+       F  F+ + +L+E G+ LP +    L  L+P ++ + L
Sbjct: 335 AVLAALPSIFPQIESVLLSPQEPFPHFKAIQNLFEEGIQLPKD--AGLLPLLP-RIIKAL 394

Query: 360 MRSDGRQFLKFPVPDVIKESKTAWRTDEEFGREMLAGVNPVIIRRLEEFPPASKLDPKAY 419
             +     L+F  P +I   + +W  D+EF R+ LAG+NP  I+ +EE+P  SKLDP  Y
Sbjct: 395 GEAQD-DILQFDAPVLINRDRFSWLRDDEFARQTLAGLNPYSIQLVEEWPLISKLDPAVY 454

Query: 420 GKQNSSITEGHIAQHLNG-LTVDQALEMNKLFILDHHDSLMPYISRINS-TSTKMYATRT 479
           G   S IT   + + + G +TVD+AL+  +LF+LD+HD L+PY++++    +T +YA+RT
Sbjct: 455 GDPTSLITWEIVEREVKGNMTVDEALKNKRLFVLDYHDLLLPYVNKVRELNNTTLYASRT 514

Query: 480 VLLLQDNGILKPLAIELSLPHPQGDHHGAVSKVFTPAEHGVEGSVWQLAKAYVAVSDSGY 539
           +  L D+  L+P+AIEL+   P   +     +VFTP        +W LAK +    D+GY
Sbjct: 515 LFFLSDDSTLRPVAIELTC--PPNINKPQWKQVFTPGYDATSCWLWNLAKTHAISHDAGY 574

Query: 540 HQLISHWLNTHAVIEPFVIATNRQLSVMHPIYKLLHPHFRDTMNINAMARQVLINAGGIL 599
           HQLISHWL THA  EP++IA NRQLS MHPIY+LLHPHFR TM INA ARQ L+N GGI+
Sbjct: 575 HQLISHWLRTHACTEPYIIAANRQLSAMHPIYRLLHPHFRYTMEINARARQSLVNGGGII 634

Query: 600 ETTVFPGKYALEMSAVLY-KNWIFTDQALPADLIKRGVAVPDPDSPHGLKLLIEDYPYAV 659
           ET  +PGKYALE+S+ +Y K W F  + LPADLIKRG+A  D  + HG++L I DYP+A 
Sbjct: 635 ETCFWPGKYALELSSAVYGKLWRFDQEGLPADLIKRGLAEEDKTAEHGVRLTIPDYPFAN 694

Query: 660 DGLEIWSAIETWVREYCCFYYKSDEMVEEDTEIQSWWTELRNVGHGDLKDEPWWPKMKTR 719
           DGL +W AI+ WV +Y   YY  +E++  D E+Q WW+E+RN+GHGD KDEPWWP +KT+
Sbjct: 695 DGLILWDAIKEWVTDYVKHYYPDEELITSDEELQGWWSEVRNIGHGDKKDEPWWPVLKTQ 754

Query: 720 EELIQSCTIVIWIASALHAAVNFGQYPYAGYLPNRPTVSRRFMP--EPGTPEFRELETDP 779
           ++LI   T + W+ S  HAAVNFGQY Y GY PNRPT +R  MP  +P     +E    P
Sbjct: 755 DDLIGVVTTIAWVTSGHHAAVNFGQYGYGGYFPNRPTTTRIRMPTEDPTDEALKEFYESP 814

Query: 780 DLAYLKTITAQLQTILGVSLIESLSRHSVDEIYLGQRETADWTSDEEAVAAFERFGDRLK 839
           +   LKT  +Q Q  L +  ++ LS HS DE Y+G+++ A W ++    AAFERF  +L+
Sbjct: 815 EKVLLKTYPSQKQATLVMVTLDLLSTHSPDEEYIGEQQEASWANEPVINAAFERFKGKLQ 874

Query: 840 EIEEKIMRMNNDERWRNRVGPVKMPYTLLFPNTSNYYEEEGLNARGIPNSISI 885
            +E  I   N +   +NR G   + Y LL P +     E G+   G+P SISI
Sbjct: 875 YLEGVIDERNVNITLKNRAGAGVVKYELLKPTS-----EHGVTGMGVPYSISI 896

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q431910.0e+0067.31Probable linoleate 9S-lipoxygenase 5 OS=Solanum tuberosum OX=4113 GN=LOX1.5 PE=2... [more]
Q9LUW00.0e+0067.84Linoleate 9S-lipoxygenase 5 OS=Arabidopsis thaliana OX=3702 GN=LOX5 PE=1 SV=2[more]
Q431900.0e+0066.98Probable linoleate 9S-lipoxygenase 4 OS=Solanum tuberosum OX=4113 GN=LOX1.4 PE=2... [more]
Q412380.0e+0066.74Linoleate 9S-lipoxygenase 6 (Fragment) OS=Solanum tuberosum OX=4113 GN=LOX1.6 PE... [more]
P384150.0e+0066.74Linoleate 9S-lipoxygenase A OS=Solanum lycopersicum OX=4081 GN=LOX1.1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1EQD30.0e+0091.65Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111436857 PE=3 SV=1[more]
A0A6J1KC570.0e+0091.08Lipoxygenase OS=Cucurbita maxima OX=3661 GN=LOC111494274 PE=3 SV=1[more]
Q9M4630.0e+0089.93Lipoxygenase OS=Cucumis sativus OX=3659 GN=lox9 PE=2 SV=1[more]
A0A5D3DC720.0e+0089.82Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold75860G00450... [more]
A0A1S3BMA30.0e+0089.82Lipoxygenase OS=Cucumis melo OX=3656 GN=LOC103491403 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022930397.10.0e+0091.65probable linoleate 9S-lipoxygenase 5 [Cucurbita moschata][more]
XP_023514286.10.0e+0091.08probable linoleate 9S-lipoxygenase 5 [Cucurbita pepo subsp. pepo][more]
XP_022999947.10.0e+0091.08probable linoleate 9S-lipoxygenase 5 [Cucurbita maxima][more]
KAG6593895.10.0e+0091.59putative linoleate 9S-lipoxygenase 5, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_038874760.10.0e+0090.61probable linoleate 9S-lipoxygenase 5 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G22400.10.0e+0067.84PLAT/LH2 domain-containing lipoxygenase family protein [more]
AT1G55020.10.0e+0064.40lipoxygenase 1 [more]
AT1G72520.12.3e-21344.26PLAT/LH2 domain-containing lipoxygenase family protein [more]
AT1G17420.15.3e-21044.03lipoxygenase 3 [more]
AT3G45140.12.0e-19343.34lipoxygenase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 823..843
NoneNo IPR availableGENE3D3.10.450.60coord: 379..512
e-value: 2.6E-56
score: 191.5
NoneNo IPR availableGENE3D2.60.60.20PLAT/LH2 domaincoord: 33..184
e-value: 1.1E-57
score: 196.7
NoneNo IPR availableGENE3D4.10.375.10coord: 185..288
e-value: 9.3E-42
score: 143.6
NoneNo IPR availableGENE3D1.20.245.10coord: 513..883
e-value: 3.7E-165
score: 551.4
NoneNo IPR availablePANTHERPTHR11771:SF126LINOLEATE 9S-LIPOXYGENASE 1coord: 36..884
IPR013819Lipoxygenase, C-terminalPRINTSPR00087LIPOXYGENASEcoord: 517..534
score: 55.79
coord: 535..552
score: 73.81
coord: 555..575
score: 71.9
IPR013819Lipoxygenase, C-terminalPFAMPF00305Lipoxygenasecoord: 192..862
e-value: 0.0
score: 1051.8
IPR013819Lipoxygenase, C-terminalPROSITEPS51393LIPOXYGENASE_3coord: 183..884
score: 308.979889
IPR001246Lipoxygenase, plantPRINTSPR00468PLTLPOXGNASEcoord: 404..423
score: 64.26
coord: 792..806
score: 70.07
coord: 470..494
score: 72.6
coord: 176..197
score: 68.86
coord: 248..267
score: 68.09
coord: 365..381
score: 57.45
coord: 215..231
score: 73.09
coord: 317..338
score: 54.64
coord: 150..168
score: 67.08
IPR001024PLAT/LH2 domainSMARTSM00308LH2_4coord: 36..181
e-value: 2.5E-51
score: 186.5
IPR001024PLAT/LH2 domainPFAMPF01477PLATcoord: 91..179
e-value: 1.0E-15
score: 58.3
IPR001024PLAT/LH2 domainPROSITEPS50095PLATcoord: 51..180
score: 21.464516
IPR027433Lipoxygenase, domain 3GENE3D4.10.372.10coord: 289..378
e-value: 7.2E-32
score: 111.6
IPR000907LipoxygenasePANTHERPTHR11771LIPOXYGENASEcoord: 36..884
IPR020833Lipoxygenase, iron binding sitePROSITEPS00711LIPOXYGENASE_1coord: 535..549
IPR020834Lipoxygenase, conserved sitePROSITEPS00081LIPOXYGENASE_2coord: 562..572
IPR042057Plant lipoxygenase, PLAT/LH2 domainCDDcd01751PLAT_LH2coord: 37..181
e-value: 6.55302E-57
score: 189.825
IPR036392PLAT/LH2 domain superfamilySUPERFAMILY49723Lipase/lipooxygenase domain (PLAT/LH2 domain)coord: 36..184
IPR036226Lipoxigenase, C-terminal domain superfamilySUPERFAMILY48484Lipoxigenasecoord: 185..884

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy01g002270.1Lcy01g002270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0031408 oxylipin biosynthetic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
molecular_function GO:0005515 protein binding
molecular_function GO:0016491 oxidoreductase activity