Lcy01g002250 (gene) Sponge gourd (P93075) v1

BLAST of Lcy01g002250 vs. ExPASy Swiss-Prot
Match: Q5XF36 (Vacuole membrane protein KMS1 OS=Arabidopsis thaliana OX=3702 GN=KMS1 PE=1 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 2.4e-165
Identity = 286/412 (69.42%), Postives = 343/412 (83.25%), Query Frame = 0

Query: 5   AASIASHDSEVKISEAELRRKQKQELENLTLVTQPFKTLKFFILGVLQYIKCSIAYILAK 64
           +A +AS  S+V IS   LR K ++E+ENLTL TQP  TLK F+   +QYIK SI+Y+LA 
Sbjct: 3   SAGVASSSSDVAIS--ALREKHEKEVENLTLTTQPLNTLKLFVEATIQYIKRSISYLLAH 62

Query: 65  GGWLLLLNLIIGAAGILILTIEGPHEKHVAEIVKYFDYGLWWVILGVASSIGLGSGLHTF 124
           GGW +L+  ++  +G L++T++GPH KHV E+++Y  YGLWW+ LGVASSIGLGSGLHTF
Sbjct: 63  GGWFILITTLLVVSGGLLVTVDGPHGKHVEEVLEYVRYGLWWIALGVASSIGLGSGLHTF 122

Query: 125 VLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPTWLEKDCSEFGPPIFT----SRV 184
           VLYLGPHI  FT+KA  CGRVDLKSA YDTIQLKR P+WL+K CSEFGPP+      SRV
Sbjct: 123 VLYLGPHIALFTLKATLCGRVDLKSAPYDTIQLKRVPSWLDKSCSEFGPPLMISAAGSRV 182

Query: 185 PLSSILPMVQVEAILWGIGTALGELPPYFISRAASLSGGRSEAMEELDASSRETNGLIST 244
           PL+SILP VQ+EAILWGIGTALGELPPYFISRAAS+SG   + MEELD SS E +G ++T
Sbjct: 183 PLTSILPQVQLEAILWGIGTALGELPPYFISRAASISGSTVDGMEELDGSSTEDSGFMAT 242

Query: 245 YLNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 304
           +LNRVKRW L+H+QHLNF T+L+LASVPNPLFDLAGIMCGQFGIPFWEFF ATL+GKAII
Sbjct: 243 HLNRVKRWLLTHSQHLNFFTVLVLASVPNPLFDLAGIMCGQFGIPFWEFFLATLIGKAII 302

Query: 305 KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGLSSILPELIAKLNAVKAKYLKAPSHV 364
           KTHIQTIFII VCNNQLLDW+ENELIWILS VPGL+S+LP L AKL+A+K KY+ APS V
Sbjct: 303 KTHIQTIFIICVCNNQLLDWMENELIWILSHVPGLASMLPGLTAKLHAMKEKYIDAPSPV 362

Query: 365 ATSAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAAL 413
            +  K KKWDFS +SIWN IVWLM++NF +KI+T+TAQR+LKK+Q+KE+A L
Sbjct: 363 PSHIKVKKWDFSFASIWNGIVWLMLLNFFVKIVTATAQRHLKKKQEKEMATL 412

BLAST of Lcy01g002250 vs. ExPASy Swiss-Prot
Match: F4I8Q7 (Vacuole membrane protein KMS2 OS=Arabidopsis thaliana OX=3702 GN=KMS2 PE=1 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 4.7e-153
Identity = 265/394 (67.26%), Postives = 320/394 (81.22%), Query Frame = 0

Query: 22  LRRKQKQELENLTLVTQPFKTLKFFILGVLQYIKCSIAYILAKGGWLLLLNLIIGAAGIL 81
           LR K +Q+LE LTL +QPFKTL+ F++ V  Y++   +Y+LA  GWL+L   I  A   L
Sbjct: 17  LREKHQQDLEKLTLTSQPFKTLRLFVVAVFLYVRRWSSYLLANVGWLILFCSIFVAFAAL 76

Query: 82  ILTIEGPHEKHVAEIVKYFDYGLWWVILGVASSIGLGSGLHTFVLYLGPHIGFFTIKAVQ 141
           ++T++GPH KHV E+ +Y  +GLWW+ LGVASSIGLGSGLHTFVLYLGPHI  FTIK +Q
Sbjct: 77  LVTLDGPHVKHVEELSEYTRFGLWWIFLGVASSIGLGSGLHTFVLYLGPHIALFTIKVMQ 136

Query: 142 CGRVDLKSAVYDTIQLKRGPTWLEKDCSEFGPPIFTSRVPLSSILPMVQVEAILWGIGTA 201
           CGRVDLKSA+YDTIQLKR P+WL+K C EFG P+F+S VPLSSILP VQ+EAILWG+GTA
Sbjct: 137 CGRVDLKSAIYDTIQLKRSPSWLDKPCHEFGSPVFSSGVPLSSILPQVQIEAILWGLGTA 196

Query: 202 LGELPPYFISRAASLSGGRSEAMEELDASSRETNGLISTYLNRVKRWFLSHAQHLNFLTI 261
           LGELPPYFISRAASLSGG+   M+EL+  S + NG I+  +N++K W LSH+Q+LNF TI
Sbjct: 197 LGELPPYFISRAASLSGGK---MKELETCSGDDNGFIAKRVNQIKSWLLSHSQYLNFFTI 256

Query: 262 LLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKTHIQTIFIIAVCNNQLLDWI 321
           L+LASVPNPLFDLAGIMCGQF  PFWEFF ATL+GKAIIKTHIQT+FII VCNNQLLDW+
Sbjct: 257 LILASVPNPLFDLAGIMCGQFEKPFWEFFLATLIGKAIIKTHIQTVFIICVCNNQLLDWV 316

Query: 322 ENELIWILSFVPGLSSILPELIAKLNAVKAKYLKAPSHVATSAKAKKWDFSVSSIWNTIV 381
           ENELI+ILSFVPG +S LPEL AKL  +K KYL A   V++    KKWD S +S+WN +V
Sbjct: 317 ENELIYILSFVPGFASALPELTAKLRLMKEKYLIASPPVSSDINVKKWDLSFASVWNGVV 376

Query: 382 WLMIMNFSIKIMTSTAQRYLKKQQDKELAALMNK 416
           WLM++NF  +I+TSTAQRYLKKQQ++EL AL NK
Sbjct: 377 WLMLLNFFGQIVTSTAQRYLKKQQEEELDALTNK 407

BLAST of Lcy01g002250 vs. ExPASy Swiss-Prot
Match: Q5R9K4 (Vacuole membrane protein 1 OS=Pongo abelii OX=9601 GN=VMP1 PE=2 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 3.8e-54
Identity = 138/389 (35.48%), Postives = 215/389 (55.27%), Query Frame = 0

Query: 21  ELRRKQKQELENLTLVTQPFKTLKFFILGVLQYIKCSIAYILAKGGWLLLLNLIIGAAGI 80
           E +R++++E +N+ L  QP  TL++F L +L  +K   + +  +   +++  L++ A  I
Sbjct: 32  EKKRREREERQNIVLWRQPLITLQYFSLEILVILKEWTSKLWHRQS-IVVSFLLLLAVLI 91

Query: 81  LILTIEGPHEKHVAEIVKYFDYGLWWVILGVASSIGLGSGLHTFVLYLGPHIGFFTIKAV 140
               +EG H+++V  I K F    +W+ LG+ SS+GLG+GLHTF+LYLGPHI   T+ A 
Sbjct: 92  ATYYVEGAHQQYVQRIEKQFLLYAYWIGLGILSSVGLGTGLHTFLLYLGPHIASVTLAAY 151

Query: 141 QCGRVDLKSAVYDTIQLKRGPTWLEKDCSEFGPPIFTSRVPLSSILPMVQVEAILWGIGT 200
           +C  V+     Y            +  C + G    T  + L SI+  V++EA +WGIGT
Sbjct: 152 ECNSVNFPEPPYPD----------QIICPDEGGTEGT--ISLWSIISKVRIEACMWGIGT 211

Query: 201 ALGELPPYFISRAASLSGGR--SEAMEELDASSRETNGLISTYLNRVKRWFLSHAQHLNF 260
           A+GELPPYF++RAA LSG     E  +E +            + +R K       Q + F
Sbjct: 212 AIGELPPYFMARAARLSGAEPDDEEYQEFEEMLEHAES-AQDFASRAKLAVQKLVQKVGF 271

Query: 261 LTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKTHIQTIFIIAVCNNQLL 320
             IL  AS+PNPLFDLAGI CG F +PFW FF ATL+GKAIIK HIQ IF+I   +  ++
Sbjct: 272 FGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAIIKMHIQKIFVIITFSKHIV 331

Query: 321 DWIENELIWILSFVPGLSSILPELIAKLNAVKAKYLKAPSHVATSAKAKKWDFSVSSIWN 380
           +    +++  +  VPG+   L +   +    + + L   S + T  + + W   +S ++ 
Sbjct: 332 E----QMVAFIGAVPGIGPSLQKPFQEYLEAQRQKLHHKSEMGT-PQGENW---LSWMFE 391

Query: 381 TIVWLMIMNFSIKIMTSTAQRYLKKQQDK 408
            +V +M+  F + I+ S AQ Y K+ Q +
Sbjct: 392 KLVVVMVCYFILSIINSMAQSYAKRIQQR 398

BLAST of Lcy01g002250 vs. ExPASy Swiss-Prot
Match: Q99KU0 (Vacuole membrane protein 1 OS=Mus musculus OX=10090 GN=Vmp1 PE=1 SV=2)

HSP 1 Score: 212.6 bits (540), Expect = 8.5e-54
Identity = 139/389 (35.73%), Postives = 214/389 (55.01%), Query Frame = 0

Query: 21  ELRRKQKQELENLTLVTQPFKTLKFFILGVLQYIKCSIAYILAKGGWLLLLNLIIGAAGI 80
           E +R+ ++E +N+ L  QP  TL++F L  L  +K   + +  +   +++  L++ AA +
Sbjct: 32  EKKRRDREERQNIVLWRQPLITLQYFSLETLVVLKEWTSKLWHRQS-IVVSFLLLLAALV 91

Query: 81  LILTIEGPHEKHVAEIVKYFDYGLWWVILGVASSIGLGSGLHTFVLYLGPHIGFFTIKAV 140
               +EG H+++V  I K F    +W+ LG+ SS+GLG+GLHTF+LYLGPHI   T+ A 
Sbjct: 92  ATYYVEGAHQQYVQRIEKQFLLYAYWIGLGILSSVGLGTGLHTFLLYLGPHIASVTLAAY 151

Query: 141 QCGRVDLKSAVY-DTIQLKRGPTWLEKDCSEFGPPIFTSRVPLSSILPMVQVEAILWGIG 200
           +C  V+     Y D I         E++ +E         + L SI+  V++EA +WGIG
Sbjct: 152 ECNSVNFPEPPYPDQIICP------EEEGAE-------GAISLWSIISKVRIEACMWGIG 211

Query: 201 TALGELPPYFISRAASLSGGRSEAMEELDASSR-ETNGLISTYLNRVKRWFLSHAQHLNF 260
           TA+GELPPYF++RAA LSG   +  E  +     E       + +R K       Q + F
Sbjct: 212 TAIGELPPYFMARAARLSGAEPDDEEYQEFEEMLEHAEAAQDFASRAKLAVQKLVQKVGF 271

Query: 261 LTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKTHIQTIFIIAVCNNQLL 320
             IL  AS+PNPLFDLAGI CG F +PFW FF ATL+GKAIIK HIQ IF+I   +  ++
Sbjct: 272 FGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAIIKMHIQKIFVIVTFSKHIV 331

Query: 321 DWIENELIWILSFVPGLSSILPELIAKLNAVKAKYLKAPSHVATSAKAKKWDFSVSSIWN 380
           +    +++  +  VPG+   L +   +    + + L   S   T  + + W   +S ++ 
Sbjct: 332 E----QMVTFIGAVPGIGPSLQKPFQEYLEAQRQKLHHRSEAGT-PQGENW---LSWMFE 391

Query: 381 TIVWLMIMNFSIKIMTSTAQRYLKKQQDK 408
            +V  M+  F + I+ S AQ Y K+ Q +
Sbjct: 392 KLVVAMVCYFVLSIINSMAQNYAKRIQQR 398

BLAST of Lcy01g002250 vs. ExPASy Swiss-Prot
Match: Q96GC9 (Vacuole membrane protein 1 OS=Homo sapiens OX=9606 GN=VMP1 PE=1 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.5e-53
Identity = 138/390 (35.38%), Postives = 217/390 (55.64%), Query Frame = 0

Query: 21  ELRRKQKQELENLTLVTQPFKTLKFFILGVLQYIKCSIAYILAKGGWLLLLNLIIGAAGI 80
           E +R++++E +N+ L  QP  TL++F L +L  +K   + +  +   +++  L++ A  I
Sbjct: 32  EKKRREREERQNIVLWRQPLITLQYFSLEILVILKEWTSKLWHRQS-IVVSFLLLLAVLI 91

Query: 81  LILTIEGPHEKHVAEIVKYFDYGLWWVILGVASSIGLGSGLHTFVLYLGPHIGFFTIKAV 140
               +EG H+++V  I K F    +W+ LG+ SS+GLG+GLHTF+LYLGPHI   T+ A 
Sbjct: 92  ATYYVEGVHQQYVQRIEKQFLLYAYWIGLGILSSVGLGTGLHTFLLYLGPHIASVTLAAY 151

Query: 141 QCGRVDLKSAVY-DTIQLKRGPTWLEKDCSEFGPPIFTSRVPLSSILPMVQVEAILWGIG 200
           +C  V+     Y D I         +++ +E         + L SI+  V++EA +WGIG
Sbjct: 152 ECNSVNFPEPPYPDQIICP------DEEGTE-------GTISLWSIISKVRIEACMWGIG 211

Query: 201 TALGELPPYFISRAASLSGGR--SEAMEELDASSRETNGLISTYLNRVKRWFLSHAQHLN 260
           TA+GELPPYF++RAA LSG     E  +E +            + +R K       Q + 
Sbjct: 212 TAIGELPPYFMARAARLSGAEPDDEEYQEFEEMLEHAES-AQDFASRAKLAVQKLVQKVG 271

Query: 261 FLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKTHIQTIFIIAVCNNQL 320
           F  IL  AS+PNPLFDLAGI CG F +PFW FF ATL+GKAIIK HIQ IF+I   +  +
Sbjct: 272 FFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAIIKMHIQKIFVIITFSKHI 331

Query: 321 LDWIENELIWILSFVPGLSSILPELIAKLNAVKAKYLKAPSHVATSAKAKKWDFSVSSIW 380
           ++    +++  +  VPG+   L +   +    + + L   S + T  + + W   +S ++
Sbjct: 332 VE----QMVAFIGAVPGIGPSLQKPFQEYLEAQRQKLHHKSEMGT-PQGENW---LSWMF 391

Query: 381 NTIVWLMIMNFSIKIMTSTAQRYLKKQQDK 408
             +V +M+  F + I+ S AQ Y K+ Q +
Sbjct: 392 EKLVVVMVCYFILSIINSMAQSYAKRIQQR 398

BLAST of Lcy01g002250 vs. ExPASy TrEMBL
Match: A0A0A0KED1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G424010 PE=4 SV=1)

HSP 1 Score: 763.1 bits (1969), Expect = 6.3e-217
Identity = 391/422 (92.65%), Postives = 401/422 (95.02%), Query Frame = 0

Query: 3   EEAASIASHDSEVKISEAELRRKQKQELENLTLVTQPFKTLKFFILGVLQYIKCSIAYIL 62
           EEAASIASHDSEV IS  ELR+KQKQELENLTLV QPFKTLKFFILGVLQY K SIAYIL
Sbjct: 5   EEAASIASHDSEVSIS--ELRKKQKQELENLTLVNQPFKTLKFFILGVLQYSKRSIAYIL 64

Query: 63  AKGGWLLLLNLIIGAAGILILTIEGPHEKHVAEIVKYFDYGLWWVILGVASSIGLGSGLH 122
           AKGGWLLLLNLIIGAAGILILTIEGPHEKHV EIVKYFDYGLWWVILGVASSIGLGSGLH
Sbjct: 65  AKGGWLLLLNLIIGAAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSGLH 124

Query: 123 TFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPTWLEKDCSEFGPPIFTSRVPL 182
           TFVLYLGPHIGFFTIKAVQCGRVDLKSA YDTIQLKRGP+WLEK+CSEFGPP+FTS+VPL
Sbjct: 125 TFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKECSEFGPPLFTSQVPL 184

Query: 183 SSILPMVQVEAILWGIGTALGELPPYFISRAASLSGGRSEAMEELDASSRETNGLISTYL 242
           SSILPMVQVEAILWGIGTALGELPPYFISRAA LSG RSEAMEELDASSRE+NG I TYL
Sbjct: 185 SSILPMVQVEAILWGIGTALGELPPYFISRAARLSGARSEAMEELDASSRESNGFIPTYL 244

Query: 243 NRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT 302
           NRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT
Sbjct: 245 NRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT 304

Query: 303 HIQTIFIIAVCNNQLLDWIENELIWILSFVPGLSSILPELIAKLNAVKAKYLKAPSH-VA 362
           HIQTIFIIAVCNNQLLDWIENELIWILSFVPG SS+LP LIAKLNA+KAKYLKAPS  + 
Sbjct: 305 HIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPGLIAKLNAIKAKYLKAPSQAMT 364

Query: 363 TSAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALMNKVPASAC 422
           T+ K KKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAAL NKVPAS C
Sbjct: 365 TNPKVKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALTNKVPASTC 424

Query: 423 SN 424
           SN
Sbjct: 425 SN 424

BLAST of Lcy01g002250 vs. ExPASy TrEMBL
Match: A0A6J1H384 (vacuole membrane protein KMS1-like OS=Cucurbita moschata OX=3662 GN=LOC111460087 PE=4 SV=1)

HSP 1 Score: 761.9 bits (1966), Expect = 1.4e-216
Identity = 388/421 (92.16%), Postives = 402/421 (95.49%), Query Frame = 0

Query: 3   EEAASIASHDSEVKISEAELRRKQKQELENLTLVTQPFKTLKFFILGVLQYIKCSIAYIL 62
           EEAASIASHDSEV IS  EL RKQK+ELENLTLVTQPFKTLKFFILGVLQY K SIAYIL
Sbjct: 5   EEAASIASHDSEVSIS--ELSRKQKEELENLTLVTQPFKTLKFFILGVLQYSKRSIAYIL 64

Query: 63  AKGGWLLLLNLIIGAAGILILTIEGPHEKHVAEIVKYFDYGLWWVILGVASSIGLGSGLH 122
           AKGGW LLL+LIIG AGILILTIEGPHEKHV EIVKYFDYGLWWVILGVASSIGLGSGLH
Sbjct: 65  AKGGWFLLLHLIIGVAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSGLH 124

Query: 123 TFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPTWLEKDCSEFGPPIFTSRVPL 182
           TFVLYLGPHIGFFTIKAVQCGRVDLKSA YDTIQLKRGP+WLEKDCSEFGP +FTS+VPL
Sbjct: 125 TFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTSQVPL 184

Query: 183 SSILPMVQVEAILWGIGTALGELPPYFISRAASLSGGRSEAMEELDASSRETNGLISTYL 242
           SSILPMVQ+EAILWGIGTALGELPPYFISRAA LSGGRSEAMEEL+ASSRETNG I TYL
Sbjct: 185 SSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELNASSRETNGFIPTYL 244

Query: 243 NRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT 302
           NRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT
Sbjct: 245 NRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT 304

Query: 303 HIQTIFIIAVCNNQLLDWIENELIWILSFVPGLSSILPELIAKLNAVKAKYLKAPSHVAT 362
           HIQTIFIIAVCNNQLLDWIENELIWILSFVPG SS+LPELI+KLNA+KAKYLKAPSH AT
Sbjct: 305 HIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHAAT 364

Query: 363 SAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALMNKVPASACS 422
           + KA KWDFSVSSIWNTIVWLMIMNFS+KIMTSTAQRYLK+QQD+ELAAL NKVPASACS
Sbjct: 365 NVKA-KWDFSVSSIWNTIVWLMIMNFSVKIMTSTAQRYLKQQQDEELAALTNKVPASACS 422

Query: 423 N 424
           N
Sbjct: 425 N 422

BLAST of Lcy01g002250 vs. ExPASy TrEMBL
Match: A0A5A7V7Q6 (Vacuole membrane protein KMS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold75860G00460 PE=4 SV=1)

HSP 1 Score: 761.5 bits (1965), Expect = 1.8e-216
Identity = 389/422 (92.18%), Postives = 400/422 (94.79%), Query Frame = 0

Query: 3   EEAASIASHDSEVKISEAELRRKQKQELENLTLVTQPFKTLKFFILGVLQYIKCSIAYIL 62
           EEAASIASHDSEV    ++LRR+QKQELENLTLV QPFKTLKFFILGVLQY K SIAYIL
Sbjct: 5   EEAASIASHDSEV----SKLRRQQKQELENLTLVNQPFKTLKFFILGVLQYSKRSIAYIL 64

Query: 63  AKGGWLLLLNLIIGAAGILILTIEGPHEKHVAEIVKYFDYGLWWVILGVASSIGLGSGLH 122
           AKGGWLLLLNLIIGAAGILILTIEGPHEKHV EIVKYFDYGLWWVILGVASSIGLGSGLH
Sbjct: 65  AKGGWLLLLNLIIGAAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSGLH 124

Query: 123 TFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPTWLEKDCSEFGPPIFTSRVPL 182
           TFVLYLGPHIGFFTIKAVQCGRVDLKSA YDTIQLKRGP+WLEKDCSEFGPP+FTSRVPL
Sbjct: 125 TFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPPLFTSRVPL 184

Query: 183 SSILPMVQVEAILWGIGTALGELPPYFISRAASLSGGRSEAMEELDASSRETNGLISTYL 242
           SSILPMVQVEAILWGIGTALGELPPYFISRAA LSG RSEAMEELDASSRE+NG I TYL
Sbjct: 185 SSILPMVQVEAILWGIGTALGELPPYFISRAARLSGARSEAMEELDASSRESNGFIPTYL 244

Query: 243 NRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT 302
           NR+KRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT
Sbjct: 245 NRMKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT 304

Query: 303 HIQTIFIIAVCNNQLLDWIENELIWILSFVPGLSSILPELIAKLNAVKAKYLKAPSH-VA 362
           HIQTIFIIAVCNNQLLDWIENELIWILSFVPG SS LP L+AKLNA+KAKYLKAPSH + 
Sbjct: 305 HIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSALPGLVAKLNAIKAKYLKAPSHAIT 364

Query: 363 TSAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALMNKVPASAC 422
           T+ K KKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAAL NKVPAS C
Sbjct: 365 TNPKVKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALTNKVPASTC 422

Query: 423 SN 424
           SN
Sbjct: 425 SN 422

BLAST of Lcy01g002250 vs. ExPASy TrEMBL
Match: A0A1S3BMX8 (vacuole membrane protein KMS1 OS=Cucumis melo OX=3656 GN=LOC103491404 PE=4 SV=1)

HSP 1 Score: 761.5 bits (1965), Expect = 1.8e-216
Identity = 389/422 (92.18%), Postives = 400/422 (94.79%), Query Frame = 0

Query: 3   EEAASIASHDSEVKISEAELRRKQKQELENLTLVTQPFKTLKFFILGVLQYIKCSIAYIL 62
           EEAASIASHDSEV    ++LRR+QKQELENLTLV QPFKTLKFFILGVLQY K SIAYIL
Sbjct: 5   EEAASIASHDSEV----SKLRRQQKQELENLTLVNQPFKTLKFFILGVLQYSKRSIAYIL 64

Query: 63  AKGGWLLLLNLIIGAAGILILTIEGPHEKHVAEIVKYFDYGLWWVILGVASSIGLGSGLH 122
           AKGGWLLLLNLIIGAAGILILTIEGPHEKHV EIVKYFDYGLWWVILGVASSIGLGSGLH
Sbjct: 65  AKGGWLLLLNLIIGAAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSGLH 124

Query: 123 TFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPTWLEKDCSEFGPPIFTSRVPL 182
           TFVLYLGPHIGFFTIKAVQCGRVDLKSA YDTIQLKRGP+WLEKDCSEFGPP+FTSRVPL
Sbjct: 125 TFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPPLFTSRVPL 184

Query: 183 SSILPMVQVEAILWGIGTALGELPPYFISRAASLSGGRSEAMEELDASSRETNGLISTYL 242
           SSILPMVQVEAILWGIGTALGELPPYFISRAA LSG RSEAMEELDASSRE+NG I TYL
Sbjct: 185 SSILPMVQVEAILWGIGTALGELPPYFISRAARLSGARSEAMEELDASSRESNGFIPTYL 244

Query: 243 NRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT 302
           NR+KRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT
Sbjct: 245 NRMKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT 304

Query: 303 HIQTIFIIAVCNNQLLDWIENELIWILSFVPGLSSILPELIAKLNAVKAKYLKAPSH-VA 362
           HIQTIFIIAVCNNQLLDWIENELIWILSFVPG SS LP L+AKLNA+KAKYLKAPSH + 
Sbjct: 305 HIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSALPGLVAKLNAIKAKYLKAPSHAIT 364

Query: 363 TSAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALMNKVPASAC 422
           T+ K KKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAAL NKVPAS C
Sbjct: 365 TNPKVKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALTNKVPASTC 422

Query: 423 SN 424
           SN
Sbjct: 425 SN 422

BLAST of Lcy01g002250 vs. ExPASy TrEMBL
Match: A0A6J1KZC5 (vacuole membrane protein KMS1-like OS=Cucurbita maxima OX=3661 GN=LOC111498967 PE=4 SV=1)

HSP 1 Score: 760.0 bits (1961), Expect = 5.3e-216
Identity = 387/421 (91.92%), Postives = 399/421 (94.77%), Query Frame = 0

Query: 3   EEAASIASHDSEVKISEAELRRKQKQELENLTLVTQPFKTLKFFILGVLQYIKCSIAYIL 62
           EEAASI SHDSEV IS  ELRRKQK+ELENLTLVTQPFKTLKFFILGVLQY K SIAYIL
Sbjct: 5   EEAASITSHDSEVSIS--ELRRKQKEELENLTLVTQPFKTLKFFILGVLQYSKHSIAYIL 64

Query: 63  AKGGWLLLLNLIIGAAGILILTIEGPHEKHVAEIVKYFDYGLWWVILGVASSIGLGSGLH 122
           AKGGW LLL+LIIG AGILILTIEGPHEKHV EIVKYFDYGLWWVILGVASSIGLGSGLH
Sbjct: 65  AKGGWFLLLHLIIGVAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSGLH 124

Query: 123 TFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPTWLEKDCSEFGPPIFTSRVPL 182
           TFVLYLGPHIGFFTIKAVQCGRVDLKSA YDTIQLKRGP+WLEKDCSEFGP +FTSRVPL
Sbjct: 125 TFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTSRVPL 184

Query: 183 SSILPMVQVEAILWGIGTALGELPPYFISRAASLSGGRSEAMEELDASSRETNGLISTYL 242
           SSILPMVQ+EAILWGIGTALGELPPYFISRAA LSGGRSEAMEELDASSRETNG I TYL
Sbjct: 185 SSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETNGFIPTYL 244

Query: 243 NRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT 302
           NRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT
Sbjct: 245 NRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT 304

Query: 303 HIQTIFIIAVCNNQLLDWIENELIWILSFVPGLSSILPELIAKLNAVKAKYLKAPSHVAT 362
           HIQTIFIIAVCNNQLLDWIENELIWILSFVPG SS+LPELI+KLNA+KAKYLKAPSH AT
Sbjct: 305 HIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHAAT 364

Query: 363 SAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALMNKVPASACS 422
           + KA KWDFSVSSIWNTIVWLMIMNF +KIMTSTAQRYLK+QQD+EL AL NKVPA ACS
Sbjct: 365 NVKA-KWDFSVSSIWNTIVWLMIMNFFVKIMTSTAQRYLKQQQDEELTALTNKVPALACS 422

Query: 423 N 424
           N
Sbjct: 425 N 422

BLAST of Lcy01g002250 vs. NCBI nr
Match: XP_023548712.1 (vacuole membrane protein KMS1-like [Cucurbita pepo subsp. pepo] >XP_023548714.1 vacuole membrane protein KMS1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 767.7 bits (1981), Expect = 5.3e-218
Identity = 391/421 (92.87%), Postives = 403/421 (95.72%), Query Frame = 0

Query: 3   EEAASIASHDSEVKISEAELRRKQKQELENLTLVTQPFKTLKFFILGVLQYIKCSIAYIL 62
           EEAASIASHDSEV IS  ELRRKQK+ELENLTLVTQPFKTLKFFILGVLQY K SIAYIL
Sbjct: 5   EEAASIASHDSEVSIS--ELRRKQKEELENLTLVTQPFKTLKFFILGVLQYSKRSIAYIL 64

Query: 63  AKGGWLLLLNLIIGAAGILILTIEGPHEKHVAEIVKYFDYGLWWVILGVASSIGLGSGLH 122
           AKGGW LLL+LIIG AGILILTIEGPHEKHV EIVKYFDYGLWWVILGVASSIGLGSGLH
Sbjct: 65  AKGGWFLLLHLIIGVAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSGLH 124

Query: 123 TFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPTWLEKDCSEFGPPIFTSRVPL 182
           TFVLYLGPHIGFFTIKAVQCGRVDLKSA YDTIQLKRGP+WLEKDCSEFGP +FTSRVPL
Sbjct: 125 TFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTSRVPL 184

Query: 183 SSILPMVQVEAILWGIGTALGELPPYFISRAASLSGGRSEAMEELDASSRETNGLISTYL 242
           SSILPMVQ+EAILWGIGTALGELPPYFISRAA LSGGRSEAMEELDASSRETNG I TYL
Sbjct: 185 SSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETNGFIPTYL 244

Query: 243 NRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT 302
           NRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT
Sbjct: 245 NRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT 304

Query: 303 HIQTIFIIAVCNNQLLDWIENELIWILSFVPGLSSILPELIAKLNAVKAKYLKAPSHVAT 362
           HIQTIFIIAVCNNQLLDWIENELIWILSFVPG SS+LPELI+KLNA+KAKYLKAPSH AT
Sbjct: 305 HIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHAAT 364

Query: 363 SAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALMNKVPASACS 422
           + KA KWDFSVSSIWNTIVWLMIMNFS+KIMTSTAQRYLK+QQD+ELAAL NKVPASACS
Sbjct: 365 NVKA-KWDFSVSSIWNTIVWLMIMNFSVKIMTSTAQRYLKQQQDEELAALTNKVPASACS 422

Query: 423 N 424
           N
Sbjct: 425 N 422

BLAST of Lcy01g002250 vs. NCBI nr
Match: KAG6574990.1 (Vacuole membrane protein KMS1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013560.1 Vacuole membrane protein KMS1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 766.1 bits (1977), Expect = 1.5e-217
Identity = 390/421 (92.64%), Postives = 403/421 (95.72%), Query Frame = 0

Query: 3   EEAASIASHDSEVKISEAELRRKQKQELENLTLVTQPFKTLKFFILGVLQYIKCSIAYIL 62
           EEAASIASHDSEV IS  ELRRKQK+ELENLTLVTQPFKTLKFFILGVLQY K SIAYIL
Sbjct: 5   EEAASIASHDSEVSIS--ELRRKQKEELENLTLVTQPFKTLKFFILGVLQYSKRSIAYIL 64

Query: 63  AKGGWLLLLNLIIGAAGILILTIEGPHEKHVAEIVKYFDYGLWWVILGVASSIGLGSGLH 122
           AKGGW LLL+LIIG AGILILTIEGPHEKHV EIVKYFDYGLWWVILGVASSIGLGSGLH
Sbjct: 65  AKGGWFLLLHLIIGVAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSGLH 124

Query: 123 TFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPTWLEKDCSEFGPPIFTSRVPL 182
           TFVLYLGPHIGFFTIKAVQCGRVDLKSA YDTIQLKRGP+WLEKDCSEFGP +FTS+VPL
Sbjct: 125 TFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKDCSEFGPAVFTSQVPL 184

Query: 183 SSILPMVQVEAILWGIGTALGELPPYFISRAASLSGGRSEAMEELDASSRETNGLISTYL 242
           SSILPMVQ+EAILWGIGTALGELPPYFISRAA LSGGRSEAMEELDASSRETNG I TYL
Sbjct: 185 SSILPMVQIEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRETNGFIPTYL 244

Query: 243 NRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT 302
           NRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT
Sbjct: 245 NRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT 304

Query: 303 HIQTIFIIAVCNNQLLDWIENELIWILSFVPGLSSILPELIAKLNAVKAKYLKAPSHVAT 362
           HIQTIFIIAVCNNQLLDWIENELIWILSFVPG SS+LPELI+KLNA+KAKYLKAPSH AT
Sbjct: 305 HIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPELISKLNAIKAKYLKAPSHAAT 364

Query: 363 SAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALMNKVPASACS 422
           + KA KWDFSVSSIWNTIVWLMIMNFS+KIMTSTAQRYLK+QQD+ELAAL NKVPASACS
Sbjct: 365 NVKA-KWDFSVSSIWNTIVWLMIMNFSVKIMTSTAQRYLKQQQDEELAALTNKVPASACS 422

Query: 423 N 424
           N
Sbjct: 425 N 422

BLAST of Lcy01g002250 vs. NCBI nr
Match: XP_038874615.1 (vacuole membrane protein KMS1 [Benincasa hispida])

HSP 1 Score: 763.1 bits (1969), Expect = 1.3e-216
Identity = 392/422 (92.89%), Postives = 401/422 (95.02%), Query Frame = 0

Query: 3   EEAASIASHDSEVKISEAELRRKQKQELENLTLVTQPFKTLKFFILGVLQYIKCSIAYIL 62
           EEAASIASHDSEV IS  ELRRKQK+ELENLTLV QPFKTLKFFILGVLQY K SIAYIL
Sbjct: 5   EEAASIASHDSEVSIS--ELRRKQKEELENLTLVNQPFKTLKFFILGVLQYSKRSIAYIL 64

Query: 63  AKGGWLLLLNLIIGAAGILILTIEGPHEKHVAEIVKYFDYGLWWVILGVASSIGLGSGLH 122
           AKGGWLLLLNLIIGAAGILILTIEGPHEKHV EIVKYFDYGLWWVILGVASSIGLGSGLH
Sbjct: 65  AKGGWLLLLNLIIGAAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSGLH 124

Query: 123 TFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPTWLEKDCSEFGPPIFTSRVPL 182
           TFVLYLGPHIGFFTIKAVQCGRVDLKSA YDTIQLKRGP+WLEK CSEFGPP+FTSRVPL
Sbjct: 125 TFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKSCSEFGPPVFTSRVPL 184

Query: 183 SSILPMVQVEAILWGIGTALGELPPYFISRAASLSGGRSEAMEELDASSRETNGLISTYL 242
           SSILPMVQVEAILWGIGTALGELPPYFISRAA LSGGRSEAMEELDASSRE+NG I TYL
Sbjct: 185 SSILPMVQVEAILWGIGTALGELPPYFISRAARLSGGRSEAMEELDASSRESNGFIPTYL 244

Query: 243 NRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT 302
           N+VKRWFLSHAQHLNF TILLLAS+PNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT
Sbjct: 245 NKVKRWFLSHAQHLNFFTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT 304

Query: 303 HIQTIFIIAVCNNQLLDWIENELIWILSFVPGLSSILPELIAKLNAVKAKYLKAPSHVA- 362
           HIQT+FIIAVCNNQLLDWIENELIWILSFVPG SSILP LIAKLNAVKAKYLKAPSHV  
Sbjct: 305 HIQTVFIIAVCNNQLLDWIENELIWILSFVPGFSSILPGLIAKLNAVKAKYLKAPSHVTI 364

Query: 363 TSAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALMNKVPASAC 422
            + KAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAAL +KVPASA 
Sbjct: 365 ANPKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALTDKVPASAF 424

Query: 423 SN 424
           SN
Sbjct: 425 SN 424

BLAST of Lcy01g002250 vs. NCBI nr
Match: XP_004140147.1 (vacuole membrane protein KMS1 [Cucumis sativus] >KGN48010.1 hypothetical protein Csa_003480 [Cucumis sativus])

HSP 1 Score: 763.1 bits (1969), Expect = 1.3e-216
Identity = 391/422 (92.65%), Postives = 401/422 (95.02%), Query Frame = 0

Query: 3   EEAASIASHDSEVKISEAELRRKQKQELENLTLVTQPFKTLKFFILGVLQYIKCSIAYIL 62
           EEAASIASHDSEV IS  ELR+KQKQELENLTLV QPFKTLKFFILGVLQY K SIAYIL
Sbjct: 5   EEAASIASHDSEVSIS--ELRKKQKQELENLTLVNQPFKTLKFFILGVLQYSKRSIAYIL 64

Query: 63  AKGGWLLLLNLIIGAAGILILTIEGPHEKHVAEIVKYFDYGLWWVILGVASSIGLGSGLH 122
           AKGGWLLLLNLIIGAAGILILTIEGPHEKHV EIVKYFDYGLWWVILGVASSIGLGSGLH
Sbjct: 65  AKGGWLLLLNLIIGAAGILILTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSGLH 124

Query: 123 TFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPTWLEKDCSEFGPPIFTSRVPL 182
           TFVLYLGPHIGFFTIKAVQCGRVDLKSA YDTIQLKRGP+WLEK+CSEFGPP+FTS+VPL
Sbjct: 125 TFVLYLGPHIGFFTIKAVQCGRVDLKSATYDTIQLKRGPSWLEKECSEFGPPLFTSQVPL 184

Query: 183 SSILPMVQVEAILWGIGTALGELPPYFISRAASLSGGRSEAMEELDASSRETNGLISTYL 242
           SSILPMVQVEAILWGIGTALGELPPYFISRAA LSG RSEAMEELDASSRE+NG I TYL
Sbjct: 185 SSILPMVQVEAILWGIGTALGELPPYFISRAARLSGARSEAMEELDASSRESNGFIPTYL 244

Query: 243 NRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT 302
           NRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT
Sbjct: 245 NRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKT 304

Query: 303 HIQTIFIIAVCNNQLLDWIENELIWILSFVPGLSSILPELIAKLNAVKAKYLKAPSH-VA 362
           HIQTIFIIAVCNNQLLDWIENELIWILSFVPG SS+LP LIAKLNA+KAKYLKAPS  + 
Sbjct: 305 HIQTIFIIAVCNNQLLDWIENELIWILSFVPGFSSVLPGLIAKLNAIKAKYLKAPSQAMT 364

Query: 363 TSAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALMNKVPASAC 422
           T+ K KKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAAL NKVPAS C
Sbjct: 365 TNPKVKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALTNKVPASTC 424

Query: 423 SN 424
           SN
Sbjct: 425 SN 424

BLAST of Lcy01g002250 vs. NCBI nr
Match: KAG6593896.1 (Vacuole membrane protein KMS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 762.3 bits (1967), Expect = 2.2e-216
Identity = 385/422 (91.23%), Postives = 405/422 (95.97%), Query Frame = 0

Query: 2   GEEAASIASHDSEVKISEAELRRKQKQELENLTLVTQPFKTLKFFILGVLQYIKCSIAYI 61
           GEEAAS+A+HDSEV IS  EL RK KQ+L+NLTLV QPFKTLKFF LGVLQY K SIAYI
Sbjct: 4   GEEAASLAAHDSEVSIS--ELCRKHKQDLDNLTLVNQPFKTLKFFTLGVLQYGKHSIAYI 63

Query: 62  LAKGGWLLLLNLIIGAAGILILTIEGPHEKHVAEIVKYFDYGLWWVILGVASSIGLGSGL 121
           LAKGGWLLLLNLIIGAAGIL+LTIEGPHEKHV EIVKYFDYGLWWVILGVASSIGLGSGL
Sbjct: 64  LAKGGWLLLLNLIIGAAGILVLTIEGPHEKHVEEIVKYFDYGLWWVILGVASSIGLGSGL 123

Query: 122 HTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPTWLEKDCSEFGPPIFTSRVP 181
           HTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGP+WLEKDCSEFGPP+FTS+VP
Sbjct: 124 HTFVLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPSWLEKDCSEFGPPVFTSQVP 183

Query: 182 LSSILPMVQVEAILWGIGTALGELPPYFISRAASLSGGRSEAMEELDASSRETNGLISTY 241
           LSSILPMVQ+EAILWGIGT+LGELPPYFISRAA LSGGRSEAMEELDASSRET+GLISTY
Sbjct: 184 LSSILPMVQIEAILWGIGTSLGELPPYFISRAARLSGGRSEAMEELDASSRETDGLISTY 243

Query: 242 LNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIK 301
           LNR+KRWFLSHAQHLNFLTILLLAS+PNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIK
Sbjct: 244 LNRIKRWFLSHAQHLNFLTILLLASIPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIK 303

Query: 302 THIQTIFIIAVCNNQLLDWIENELIWILSFVPGLSSILPELIAKLNAVKAKYLKAPSHVA 361
           THIQTIFIIAVCNNQLLDWIENEL+WILSFVPG SSILP L+AKLNAVK+KYLKAPS VA
Sbjct: 304 THIQTIFIIAVCNNQLLDWIENELLWILSFVPGFSSILPGLVAKLNAVKSKYLKAPSQVA 363

Query: 362 TSAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAALMNKVPASAC 421
            +AKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQD+ELAA+ +KV ASAC
Sbjct: 364 ANAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDEELAAMTDKVSASAC 423

Query: 422 SN 424
           SN
Sbjct: 424 SN 423

BLAST of Lcy01g002250 vs. TAIR 10
Match: AT4G14950.1 (SNARE associated Golgi protein family )

HSP 1 Score: 583.2 bits (1502), Expect = 1.7e-166
Identity = 286/412 (69.42%), Postives = 343/412 (83.25%), Query Frame = 0

Query: 5   AASIASHDSEVKISEAELRRKQKQELENLTLVTQPFKTLKFFILGVLQYIKCSIAYILAK 64
           +A +AS  S+V IS   LR K ++E+ENLTL TQP  TLK F+   +QYIK SI+Y+LA 
Sbjct: 3   SAGVASSSSDVAIS--ALREKHEKEVENLTLTTQPLNTLKLFVEATIQYIKRSISYLLAH 62

Query: 65  GGWLLLLNLIIGAAGILILTIEGPHEKHVAEIVKYFDYGLWWVILGVASSIGLGSGLHTF 124
           GGW +L+  ++  +G L++T++GPH KHV E+++Y  YGLWW+ LGVASSIGLGSGLHTF
Sbjct: 63  GGWFILITTLLVVSGGLLVTVDGPHGKHVEEVLEYVRYGLWWIALGVASSIGLGSGLHTF 122

Query: 125 VLYLGPHIGFFTIKAVQCGRVDLKSAVYDTIQLKRGPTWLEKDCSEFGPPIFT----SRV 184
           VLYLGPHI  FT+KA  CGRVDLKSA YDTIQLKR P+WL+K CSEFGPP+      SRV
Sbjct: 123 VLYLGPHIALFTLKATLCGRVDLKSAPYDTIQLKRVPSWLDKSCSEFGPPLMISAAGSRV 182

Query: 185 PLSSILPMVQVEAILWGIGTALGELPPYFISRAASLSGGRSEAMEELDASSRETNGLIST 244
           PL+SILP VQ+EAILWGIGTALGELPPYFISRAAS+SG   + MEELD SS E +G ++T
Sbjct: 183 PLTSILPQVQLEAILWGIGTALGELPPYFISRAASISGSTVDGMEELDGSSTEDSGFMAT 242

Query: 245 YLNRVKRWFLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAII 304
           +LNRVKRW L+H+QHLNF T+L+LASVPNPLFDLAGIMCGQFGIPFWEFF ATL+GKAII
Sbjct: 243 HLNRVKRWLLTHSQHLNFFTVLVLASVPNPLFDLAGIMCGQFGIPFWEFFLATLIGKAII 302

Query: 305 KTHIQTIFIIAVCNNQLLDWIENELIWILSFVPGLSSILPELIAKLNAVKAKYLKAPSHV 364
           KTHIQTIFII VCNNQLLDW+ENELIWILS VPGL+S+LP L AKL+A+K KY+ APS V
Sbjct: 303 KTHIQTIFIICVCNNQLLDWMENELIWILSHVPGLASMLPGLTAKLHAMKEKYIDAPSPV 362

Query: 365 ATSAKAKKWDFSVSSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAAL 413
            +  K KKWDFS +SIWN IVWLM++NF +KI+T+TAQR+LKK+Q+KE+A L
Sbjct: 363 PSHIKVKKWDFSFASIWNGIVWLMLLNFFVKIVTATAQRHLKKKQEKEMATL 412

BLAST of Lcy01g002250 vs. TAIR 10
Match: AT4G14950.3 (SNARE associated Golgi protein family )

HSP 1 Score: 580.5 bits (1495), Expect = 1.1e-165
Identity = 281/399 (70.43%), Postives = 336/399 (84.21%), Query Frame = 0

Query: 18  SEAELRRKQKQELENLTLVTQPFKTLKFFILGVLQYIKCSIAYILAKGGWLLLLNLIIGA 77
           S+A LR K ++E+ENLTL TQP  TLK F+   +QYIK SI+Y+LA GGW +L+  ++  
Sbjct: 4   SQATLREKHEKEVENLTLTTQPLNTLKLFVEATIQYIKRSISYLLAHGGWFILITTLLVV 63

Query: 78  AGILILTIEGPHEKHVAEIVKYFDYGLWWVILGVASSIGLGSGLHTFVLYLGPHIGFFTI 137
           +G L++T++GPH KHV E+++Y  YGLWW+ LGVASSIGLGSGLHTFVLYLGPHI  FT+
Sbjct: 64  SGGLLVTVDGPHGKHVEEVLEYVRYGLWWIALGVASSIGLGSGLHTFVLYLGPHIALFTL 123

Query: 138 KAVQCGRVDLKSAVYDTIQLKRGPTWLEKDCSEFGPPIFT----SRVPLSSILPMVQVEA 197
           KA  CGRVDLKSA YDTIQLKR P+WL+K CSEFGPP+      SRVPL+SILP VQ+EA
Sbjct: 124 KATLCGRVDLKSAPYDTIQLKRVPSWLDKSCSEFGPPLMISAAGSRVPLTSILPQVQLEA 183

Query: 198 ILWGIGTALGELPPYFISRAASLSGGRSEAMEELDASSRETNGLISTYLNRVKRWFLSHA 257
           ILWGIGTALGELPPYFISRAAS+SG   + MEELD SS E +G ++T+LNRVKRW L+H+
Sbjct: 184 ILWGIGTALGELPPYFISRAASISGSTVDGMEELDGSSTEDSGFMATHLNRVKRWLLTHS 243

Query: 258 QHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKTHIQTIFIIAVC 317
           QHLNF T+L+LASVPNPLFDLAGIMCGQFGIPFWEFF ATL+GKAIIKTHIQTIFII VC
Sbjct: 244 QHLNFFTVLVLASVPNPLFDLAGIMCGQFGIPFWEFFLATLIGKAIIKTHIQTIFIICVC 303

Query: 318 NNQLLDWIENELIWILSFVPGLSSILPELIAKLNAVKAKYLKAPSHVATSAKAKKWDFSV 377
           NNQLLDW+ENELIWILS VPGL+S+LP L AKL+A+K KY+ APS V +  K KKWDFS 
Sbjct: 304 NNQLLDWMENELIWILSHVPGLASMLPGLTAKLHAMKEKYIDAPSPVPSHIKVKKWDFSF 363

Query: 378 SSIWNTIVWLMIMNFSIKIMTSTAQRYLKKQQDKELAAL 413
           +SIWN IVWLM++NF +KI+T+TAQR+LKK+Q+KE+A L
Sbjct: 364 ASIWNGIVWLMLLNFFVKIVTATAQRHLKKKQEKEMATL 402

BLAST of Lcy01g002250 vs. TAIR 10
Match: AT1G05360.1 (BEST Arabidopsis thaliana protein match is: SNARE associated Golgi protein family (TAIR:AT4G14950.1); Has 332 Blast hits to 326 proteins in 115 species: Archae - 0; Bacteria - 11; Metazoa - 174; Fungi - 0; Plants - 74; Viruses - 0; Other Eukaryotes - 73 (source: NCBI BLink). )

HSP 1 Score: 542.3 bits (1396), Expect = 3.3e-154
Identity = 265/394 (67.26%), Postives = 320/394 (81.22%), Query Frame = 0

Query: 22  LRRKQKQELENLTLVTQPFKTLKFFILGVLQYIKCSIAYILAKGGWLLLLNLIIGAAGIL 81
           LR K +Q+LE LTL +QPFKTL+ F++ V  Y++   +Y+LA  GWL+L   I  A   L
Sbjct: 17  LREKHQQDLEKLTLTSQPFKTLRLFVVAVFLYVRRWSSYLLANVGWLILFCSIFVAFAAL 76

Query: 82  ILTIEGPHEKHVAEIVKYFDYGLWWVILGVASSIGLGSGLHTFVLYLGPHIGFFTIKAVQ 141
           ++T++GPH KHV E+ +Y  +GLWW+ LGVASSIGLGSGLHTFVLYLGPHI  FTIK +Q
Sbjct: 77  LVTLDGPHVKHVEELSEYTRFGLWWIFLGVASSIGLGSGLHTFVLYLGPHIALFTIKVMQ 136

Query: 142 CGRVDLKSAVYDTIQLKRGPTWLEKDCSEFGPPIFTSRVPLSSILPMVQVEAILWGIGTA 201
           CGRVDLKSA+YDTIQLKR P+WL+K C EFG P+F+S VPLSSILP VQ+EAILWG+GTA
Sbjct: 137 CGRVDLKSAIYDTIQLKRSPSWLDKPCHEFGSPVFSSGVPLSSILPQVQIEAILWGLGTA 196

Query: 202 LGELPPYFISRAASLSGGRSEAMEELDASSRETNGLISTYLNRVKRWFLSHAQHLNFLTI 261
           LGELPPYFISRAASLSGG+   M+EL+  S + NG I+  +N++K W LSH+Q+LNF TI
Sbjct: 197 LGELPPYFISRAASLSGGK---MKELETCSGDDNGFIAKRVNQIKSWLLSHSQYLNFFTI 256

Query: 262 LLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKTHIQTIFIIAVCNNQLLDWI 321
           L+LASVPNPLFDLAGIMCGQF  PFWEFF ATL+GKAIIKTHIQT+FII VCNNQLLDW+
Sbjct: 257 LILASVPNPLFDLAGIMCGQFEKPFWEFFLATLIGKAIIKTHIQTVFIICVCNNQLLDWV 316

Query: 322 ENELIWILSFVPGLSSILPELIAKLNAVKAKYLKAPSHVATSAKAKKWDFSVSSIWNTIV 381
           ENELI+ILSFVPG +S LPEL AKL  +K KYL A   V++    KKWD S +S+WN +V
Sbjct: 317 ENELIYILSFVPGFASALPELTAKLRLMKEKYLIASPPVSSDINVKKWDLSFASVWNGVV 376

Query: 382 WLMIMNFSIKIMTSTAQRYLKKQQDKELAALMNK 416
           WLM++NF  +I+TSTAQRYLKKQQ++EL AL NK
Sbjct: 377 WLMLLNFFGQIVTSTAQRYLKKQQEEELDALTNK 407

BLAST of Lcy01g002250 vs. TAIR 10
Match: AT4G14950.2 (SNARE associated Golgi protein family )

HSP 1 Score: 512.7 bits (1319), Expect = 2.8e-145
Identity = 249/352 (70.74%), Postives = 294/352 (83.52%), Query Frame = 0

Query: 18  SEAELRRKQKQELENLTLVTQPFKTLKFFILGVLQYIKCSIAYILAKGGWLLLLNLIIGA 77
           S+A LR K ++E+ENLTL TQP  TLK F+   +QYIK SI+Y+LA GGW +L+  ++  
Sbjct: 4   SQATLREKHEKEVENLTLTTQPLNTLKLFVEATIQYIKRSISYLLAHGGWFILITTLLVV 63

Query: 78  AGILILTIEGPHEKHVAEIVKYFDYGLWWVILGVASSIGLGSGLHTFVLYLGPHIGFFTI 137
           +G L++T++GPH KHV E+++Y  YGLWW+ LGVASSIGLGSGLHTFVLYLGPHI  FT+
Sbjct: 64  SGGLLVTVDGPHGKHVEEVLEYVRYGLWWIALGVASSIGLGSGLHTFVLYLGPHIALFTL 123

Query: 138 KAVQCGRVDLKSAVYDTIQLKRGPTWLEKDCSEFGPPIFT----SRVPLSSILPMVQVEA 197
           KA  CGRVDLKSA YDTIQLKR P+WL+K CSEFGPP+      SRVPL+SILP VQ+EA
Sbjct: 124 KATLCGRVDLKSAPYDTIQLKRVPSWLDKSCSEFGPPLMISAAGSRVPLTSILPQVQLEA 183

Query: 198 ILWGIGTALGELPPYFISRAASLSGGRSEAMEELDASSRETNGLISTYLNRVKRWFLSHA 257
           ILWGIGTALGELPPYFISRAAS+SG   + MEELD SS E +G ++T+LNRVKRW L+H+
Sbjct: 184 ILWGIGTALGELPPYFISRAASISGSTVDGMEELDGSSTEDSGFMATHLNRVKRWLLTHS 243

Query: 258 QHLNFLTILLLASVPNPLFDLAGIMCGQFGIPFWEFFFATLVGKAIIKTHIQTIFIIAVC 317
           QHLNF T+L+LASVPNPLFDLAGIMCGQFGIPFWEFF ATL+GKAIIKTHIQTIFII VC
Sbjct: 244 QHLNFFTVLVLASVPNPLFDLAGIMCGQFGIPFWEFFLATLIGKAIIKTHIQTIFIICVC 303

Query: 318 NNQLLDWIENELIWILSFVPGLSSILPELIAKLNAVKAKYLKAPSHVATSAK 366
           NNQLLDW+ENELIWILS VPGL+S+LP L AKL+A+K KY+ APS V +  K
Sbjct: 304 NNQLLDWMENELIWILSHVPGLASMLPGLTAKLHAMKEKYIDAPSPVPSHIK 355