Lcy01g002090 (gene) Sponge gourd (P93075) v1

Overview
NameLcy01g002090
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptionmitogen-activated protein kinase kinase kinase NPK1
LocationChr01: 2440864 .. 2445971 (+)
RNA-Seq ExpressionLcy01g002090
SyntenyLcy01g002090
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCGACCGTTGTTTTCTCCATTCAAAATCCCACTCTCCCACAGTGAGAATGGTTCTCATAGAACCCTAAACACTCATTTCCCCCAAATTCCATCTCCACACAGATTCCATCAATTTCTAGAGATCTGAGAGTCTTCACGACGATGCAAGATTTCTTCGGATCCGTTCGCCGATCCTTGGTGTTTCTAGCTCCCGACTGTGACGACGGTGGGAGGTTCGGCGGCCTGGTCGAGAAGATCGGCTCGAGCATCCGTAAATCTCGAAATGGACTGTTTTCTAAGCATTCAGTGCGGGCTCTTCCTCCGGTTGTGAAGGAAGATGCGCCTCCGATTTGGTGGCGGAAGGGAGAGTTGATCGGATGCGGTGCTTTTGGTCGAGTTTATATGGGGATGAATCTTGATTCTGGAGAGCTTCTTGCAGTTAAGCAAGTTAGTAATTGTTTTAGATTTTGTTTGTGAAGTTTATTTAGTTGGTAATCGGTTTTGAACTTTTGAGAACTTTTGATTCTTAAATTCCTCTGTATCATTGCAGGTCTCGATTGCGGCTAATAGTGCTTCAAGGGAGAAAGCGCAAGTAAGGACGTTTACTGATTTGAAGTTTGCTAGATGTGGATCACTTGCAGTAGAAATTAGCTAGCAATTTTTCTATACCTTTGTTGGTTCTGATTGAATTCACCGAACACACTCTCACGCTTTCTGTTTAATATAACTATTCAGGCTCACATACGGGAGCTGGAGGAGGAAGTGAAGCTTTTGAAAAATCTTTCTCACCCTAATATTGTGGTAAGTAAAATCTATTAGTCTTTCATTCTCTTCTAGTGTTGTTGGTAGCTCCTTGCTTATTTTCCTTGTGATTTTAGAGATACCTGGGAACTGCCAGAGAGGAAGATTCATTAAATATTCTATTGGAGTTTGTTCCTGGAGGATCAATATCATCTCTGTTAGGGAAATTCGGATCTTTCCCTGAGTCTGTAAGTGATTGTACATGTGTATCTTTAGTCTTTGACCCTCTCAGTATTGAAATAGAGTAAACTTAATGTTGAAATTTTGCAAATGGTTATTGATTTTGTCTAAAATTAGGTTATAAGAACCTACACGAAGCAGCTATTATTGGGGCTTGAGTACCTTCATAAGAATGGCATTATGCATCGGGATATAAAGGTGATAGTGAAACCCCTTTTTGAGAAAGAAGAGTTTTAACTTTTAACCTTTTTTTTTTTTTTTTTTGTTAGTTTAACTGAATGATGTGCTACTTGACATAGGGTGCAAATATTCTTGTAGACAATAAAGGATGTATTAAACTCGCTGATTTTGGTGCATCCAAGAAAGTGGTTGAACTGGTACTGCCCATTGTTTATTTAATTTAATTATCTAAATTAATTGTTATCATTCTCAACGTAAAAATTAGCATCTATGCAGGCAACTATAAATGGAGCCAAATCAATGAAGGGCACTCCATATTGGATGGCTCCTGAAGTTATTCTCCAGACCGGACATAGCTTGTAAGAATCATCATCCTATTGTGAACAGTTTTATTGGCTTTCACTGTTTCACTCCATTCCATTGACATGGAAACTATTTAAATGCATAGGAAGAATACAGATGTGTTGAAATCTAAACCATGCCATTGATGTGGATCAATATGTTGTAATGCATGGGAGAAATACAAATGTGCTGAAACTTATTCTGAAATAATGGAGATGTAACAAAAGTAGCAGGAGAAAAACATCAAGTTTTCTTGTTAAGAAACTAAATTTTAGATCTCATGTTGTTCTTAATTTGATGTCTTAGCTCTGCTGATATATGGAGTGTAGGGGGGTGTGCTGTTTTTGAAATGGCAACTGGAAAACCTCCATGTGCTGAAACTTATTTCGAAATGATGGAGATGCAACAAAAGTAGCAAGAGAAAAACATGAAGTTTTCTTTATCAAGAAATCTCACGTTGTTCTTAATTTGATGTCTCAGCTCTGCTGATATATGGAGTGTGGGGTGTACTGTTATCGAAATGGCAACGGGGAAACCTCCATGGAGCCAACAGTATCAAGAGGTTAGCAAGTGAATTTTCTTCAATATGTATGTATGTGCTTCTTACATTTTCTTCAAATATTTTATAAATCGAGTGAATGCTATTTTCAGGTTGCTGCTCTTTTCCATATAGGGAATACTAAGTCCCATCCGCCCATTCCTGAGCACCTATCTGCTGAGGCAAAAGATTTTCTGTTAAAATGTTTACAAGAGTAACTTTCGAAACTCTGACTTTAGTTTTTCACTTGACTTTTTTTCCCCTGGAAGCGTCAATATACTTCGCCATTAATAAGATTGATCGAAACATATACCCTATTCTCTGTTTAATTTAACTTCATCTAATTATAACCATCCAAATGTGCTCACAGGGAACCAAACTCAAGGCCCACTGCATCAGACTTGCTGCAGGTATATCTGAATTATTACAGTGTATCTTATCAATTGGGCATAAAGACTTGCATTTATTTTTTGTTGGAGATAGTAGCTCTATTCTTACCCATTTTCTATGGATATGTAGCATCCATTTGTCTCATGTGAGTATCAAGATCCTCATGCAGCAGTCCGTGCCTCTTCCGTGGTCTGTATATTTTCTGTGTAACTTTAGATGTTTTTACCATCTGTATCAAAAAATATATATTTTTACCATCCCATCTTAATTTAATTGCTATTGCAGGAATCTGGGAAACCGATGGTTGATTCTAGATTGAATTCTAATGATCTGTAAGGAGTTCAATTGATGATCATTGAATATCTGATTTCAGTTGATGATTTTTGTTGCCCACTATCCAAACTGACTCTAGACTGATGATTTTTGTTGGCCATGGCAGAACAAATTCTACAATCCAACGTTCAACTGGCTTGAAGGATATTTGTGAAATGGGTAGCTTGAGGTGCTCTTCTGTATTTTCCGGGAATTATGGGTCACGGTCCAACTGGGGCTCCAGCAATTTTGAGGATGACATGTGCCAGATAGATGATAAAGATCTATTTGTTAGTTCATCAATAAAGTACAAATCTATTATATCATCACACGATTTGAATAAGGTACACATCGATTCTCTGATTATGTTATTTGTTGGTTTTATTAGCATCAAGAATAATGAGCAGCTTTTCATTTGAAACAGAGTTTCAATCCTATGTGTGAACCAACTGATGACTGGGCATGTAAATTGGATGAGAGCTCAGAGTTGGGAGGCAATTTAATGGAATTGTCTGGCATTCAAACTGGCCAGGAATCTACTAGTAAGGGGAATGACTTCACTTTTCCATGTGGTCAATCCGCAGCTGAGGATGATGAAGAAGTTACAGAGTCGAAGATTAAAGCCTTTCTGGATGAGAAGGTACTTCTATTCACCAAGATATTTTTAAGTTTGCAAAATGCAGCGTGATGTTTTTTACATCAGAATTAATGTTTCTACTGTGAAATAAACATCAGGCTTTAGAACTGAAGAAGCTGCAAACACCTTTATATGAAGAGTTCCGCAGCACAATCAATGCAGCTAATGCTTTAGGAGCAGTTGGAACTGAGAATAGCAAGAGTGTATCTAATCTTCTGAAATTGCCTCCCAAATGTGGGTCACCAAGTAAGCTGCGTGGTAAAAGACTCTCATCAGTTGATGCTGCTAACTGTTCAAGCCGTCAGAGTCGTATAAAACAAGATAGCATATTGCAAGATCGAGCTCTGCAGGAAATTCAGTCGCCTCAGCTTGGGGAATGGAACAAATTGCTTCATACACAGCCAGAGTCTTGCACGCCAAGGTTGCAATTTAGTAGCTTATATTTCTAGCTCATTTGTGGTAAGTTAATTTAACAGTACAATAATGGAAAACTTGCTTGCAGTTTCTCTGAGAGACAAAGGAAATGGAAAGAAGAGCTTGAAGAAGAACTTAAAAGGAAGCGAGGTTAGTTTACTTAAATCTTATGATATTAAGCCAGAGGTTTTGTCGGAATTTCATCTCGTCTGTTAAGTGAATTCTGTGGTGGTTTATCATCTGTGTGTAGATTAGCATGAACTGAATTGTTCATGCTGAAAGCTACAATACAGTGAAACTAGTTCCCAACTATCTATTGAAATCCCGTTTTCCTTTCATAGTCTAATTGAGTCAACATAAGCTTCAGAGTTTGGATGTTTTGTCCACCTTAAAAATGGGCATGTGAAATCAATGTACTTGTGAACCATGCGCCATTGTGTTACAACCATTATCCAGTATTCCAGTTTTTGATGGTCAGCAGAGGTGGGAAGAAAAGGATATATATGGTTGGTATGACTGTGTGAGGGCACTCCCATATTCTCACCTGAAAACAAGTCTAGAATGTAATGTATTTGAGAAGAAAAGAATAGATATCACATAGAAAGCAATTAAATAAAACAAATAATCATGGCTTATTAAGATACTAATAAATATCATATTAGCCATTAGGTTTCAGTGTTTTCTGCATCTTTGCATAATTATATTCGGATAGTGTGATGATGGAAATGCTGTGCTTATTGCAGAAATGATGCGGAGAGGAATTGTAGGAGCAATTCACTAAAACCATCATCTTCAAAGGATCAGAGAGTAAAAACAACAAAAATCACGGTGAATCTCGCATCCCCTGGCAGTAAAATATTGTGTAGCCTCTATGGATATCTGTTGTACTATGCAGGGGATAAAAGTGTCTTCTCATGGTTACAAGTGTTAGAAATGTATGTACACATAATCATCAGCAGCAAAGTTCAGTAAAGGTGGATGCTCCACATGCTGTTCATGGTCTCAGCACTGAGCACAATTCTCACAAGGGAGTTCAGGCATTTCAACTAGAAAAATCTGATAAGTAATATATTTACATTTATTTCATTATTACATTAATATTTGCCTCCTCCTCCCTCATCTGACCAGCAGCTGTCTGCCTTCCCCACCCCCGCCGCGCCCGGTGGCAGTGGCGGCGGTAGTGGTTCCTATTATTTTCTATGTTATTGGTGGAAATTTGTGAGTTAGGAGAGAGTTTTTTATTTGGTATTATTCCAAGTGTGAATCATATTACAATGTGTATTTTTTAAGTGTCTTCTGTCTATTGTCTCAATGATAGATATCTCTTTTTACAA

mRNA sequence

TTCGACCGTTGTTTTCTCCATTCAAAATCCCACTCTCCCACAGTGAGAATGGTTCTCATAGAACCCTAAACACTCATTTCCCCCAAATTCCATCTCCACACAGATTCCATCAATTTCTAGAGATCTGAGAGTCTTCACGACGATGCAAGATTTCTTCGGATCCGTTCGCCGATCCTTGGTGTTTCTAGCTCCCGACTGTGACGACGGTGGGAGGTTCGGCGGCCTGGTCGAGAAGATCGGCTCGAGCATCCGTAAATCTCGAAATGGACTGTTTTCTAAGCATTCAGTGCGGGCTCTTCCTCCGGTTGTGAAGGAAGATGCGCCTCCGATTTGGTGGCGGAAGGGAGAGTTGATCGGATGCGGTGCTTTTGGTCGAGTTTATATGGGGATGAATCTTGATTCTGGAGAGCTTCTTGCAGTTAAGCAAGTCTCGATTGCGGCTAATAGTGCTTCAAGGGAGAAAGCGCAAGCTCACATACGGGAGCTGGAGGAGGAAGTGAAGCTTTTGAAAAATCTTTCTCACCCTAATATTGTGAGATACCTGGGAACTGCCAGAGAGGAAGATTCATTAAATATTCTATTGGAGTTTGTTCCTGGAGGATCAATATCATCTCTGTTAGGGAAATTCGGATCTTTCCCTGAGTCTGTTATAAGAACCTACACGAAGCAGCTATTATTGGGGCTTGAGTACCTTCATAAGAATGGCATTATGCATCGGGATATAAAGGGTGCAAATATTCTTGTAGACAATAAAGGATGTATTAAACTCGCTGATTTTGGTGCATCCAAGAAAGTGGTTGAACTGGCAACTATAAATGGAGCCAAATCAATGAAGGGCACTCCATATTGGATGGCTCCTGAAGTTATTCTCCAGACCGGACATAGCTTCTCTGCTGATATATGGAGTGTGGGGTGTACTGTTATCGAAATGGCAACGGGGAAACCTCCATGGAGCCAACAGTATCAAGAGGTTGCTGCTCTTTTCCATATAGGGAATACTAAGTCCCATCCGCCCATTCCTGAGCACCTATCTGCTGAGGCAAAAGATTTTCTGTTAAAATGTTTACAAGAGGAACCAAACTCAAGGCCCACTGCATCAGACTTGCTGCAGCATCCATTTGTCTCATGTGAGTATCAAGATCCTCATGCAGCAGTCCGTGCCTCTTCCGTGGAATCTGGGAAACCGATGGTTGATTCTAGATTGAATTCTAATGATCTAACAAATTCTACAATCCAACGTTCAACTGGCTTGAAGGATATTTGTGAAATGGGTAGCTTGAGGTGCTCTTCTGTATTTTCCGGGAATTATGGGTCACGGTCCAACTGGGGCTCCAGCAATTTTGAGGATGACATGTGCCAGATAGATGATAAAGATCTATTTGTTAGTTCATCAATAAAGTACAAATCTATTATATCATCACACGATTTGAATAAGAGTTTCAATCCTATGTGTGAACCAACTGATGACTGGGCATGTAAATTGGATGAGAGCTCAGAGTTGGGAGGCAATTTAATGGAATTGTCTGGCATTCAAACTGGCCAGGAATCTACTAGTAAGGGGAATGACTTCACTTTTCCATGTGGTCAATCCGCAGCTGAGGATGATGAAGAAGTTACAGAGTCGAAGATTAAAGCCTTTCTGGATGAGAAGGCTTTAGAACTGAAGAAGCTGCAAACACCTTTATATGAAGAGTTCCGCAGCACAATCAATGCAGCTAATGCTTTAGGAGCAGTTGGAACTGAGAATAGCAAGAGTGTATCTAATCTTCTGAAATTGCCTCCCAAATGTGGGTCACCAAGTAAGCTGCGTGGTAAAAGACTCTCATCAGTTGATGCTGCTAACTGTTCAAGCCGTCAGAGTCGTATAAAACAAGATAGCATATTGCAAGATCGAGCTCTGCAGGAAATTCAGTCGCCTCAGCTTGGGGAATGGAACAAATTGCTTCATACACAGCCAGAGTCTTGCACGCCAAGTTTCTCTGAGAGACAAAGGAAATGGAAAGAAGAGCTTGAAGAAGAACTTAAAAGGAAGCGAGAAATGATGCGGAGAGGAATTGTAGGAGCAATTCACTAAAACCATCATCTTCAAAGGATCAGAGAGTAAAAACAACAAAAATCACGGTGAATCTCGCATCCCCTGGCAGTAAAATATTGTGTAGCCTCTATGGATATCTGTTGTACTATGCAGGGGATAAAAGTGTCTTCTCATGGTTACAAGTGTTAGAAATGTATGTACACATAATCATCAGCAGCAAAGTTCAGTAAAGGTGGATGCTCCACATGCTGTTCATGGTCTCAGCACTGAGCACAATTCTCACAAGGGAGTTCAGGCATTTCAACTAGAAAAATCTGATAAGTAATATATTTACATTTATTTCATTATTACATTAATATTTGCCTCCTCCTCCCTCATCTGACCAGCAGCTGTCTGCCTTCCCCACCCCCGCCGCGCCCGGTGGCAGTGGCGGCGGTAGTGGTTCCTATTATTTTCTATGTTATTGGTGGAAATTTGTGAGTTAGGAGAGAGTTTTTTATTTGGTATTATTCCAAGTGTGAATCATATTACAATGTGTATTTTTTAAGTGTCTTCTGTCTATTGTCTCAATGATAGATATCTCTTTTTACAA

Coding sequence (CDS)

ATGCAAGATTTCTTCGGATCCGTTCGCCGATCCTTGGTGTTTCTAGCTCCCGACTGTGACGACGGTGGGAGGTTCGGCGGCCTGGTCGAGAAGATCGGCTCGAGCATCCGTAAATCTCGAAATGGACTGTTTTCTAAGCATTCAGTGCGGGCTCTTCCTCCGGTTGTGAAGGAAGATGCGCCTCCGATTTGGTGGCGGAAGGGAGAGTTGATCGGATGCGGTGCTTTTGGTCGAGTTTATATGGGGATGAATCTTGATTCTGGAGAGCTTCTTGCAGTTAAGCAAGTCTCGATTGCGGCTAATAGTGCTTCAAGGGAGAAAGCGCAAGCTCACATACGGGAGCTGGAGGAGGAAGTGAAGCTTTTGAAAAATCTTTCTCACCCTAATATTGTGAGATACCTGGGAACTGCCAGAGAGGAAGATTCATTAAATATTCTATTGGAGTTTGTTCCTGGAGGATCAATATCATCTCTGTTAGGGAAATTCGGATCTTTCCCTGAGTCTGTTATAAGAACCTACACGAAGCAGCTATTATTGGGGCTTGAGTACCTTCATAAGAATGGCATTATGCATCGGGATATAAAGGGTGCAAATATTCTTGTAGACAATAAAGGATGTATTAAACTCGCTGATTTTGGTGCATCCAAGAAAGTGGTTGAACTGGCAACTATAAATGGAGCCAAATCAATGAAGGGCACTCCATATTGGATGGCTCCTGAAGTTATTCTCCAGACCGGACATAGCTTCTCTGCTGATATATGGAGTGTGGGGTGTACTGTTATCGAAATGGCAACGGGGAAACCTCCATGGAGCCAACAGTATCAAGAGGTTGCTGCTCTTTTCCATATAGGGAATACTAAGTCCCATCCGCCCATTCCTGAGCACCTATCTGCTGAGGCAAAAGATTTTCTGTTAAAATGTTTACAAGAGGAACCAAACTCAAGGCCCACTGCATCAGACTTGCTGCAGCATCCATTTGTCTCATGTGAGTATCAAGATCCTCATGCAGCAGTCCGTGCCTCTTCCGTGGAATCTGGGAAACCGATGGTTGATTCTAGATTGAATTCTAATGATCTAACAAATTCTACAATCCAACGTTCAACTGGCTTGAAGGATATTTGTGAAATGGGTAGCTTGAGGTGCTCTTCTGTATTTTCCGGGAATTATGGGTCACGGTCCAACTGGGGCTCCAGCAATTTTGAGGATGACATGTGCCAGATAGATGATAAAGATCTATTTGTTAGTTCATCAATAAAGTACAAATCTATTATATCATCACACGATTTGAATAAGAGTTTCAATCCTATGTGTGAACCAACTGATGACTGGGCATGTAAATTGGATGAGAGCTCAGAGTTGGGAGGCAATTTAATGGAATTGTCTGGCATTCAAACTGGCCAGGAATCTACTAGTAAGGGGAATGACTTCACTTTTCCATGTGGTCAATCCGCAGCTGAGGATGATGAAGAAGTTACAGAGTCGAAGATTAAAGCCTTTCTGGATGAGAAGGCTTTAGAACTGAAGAAGCTGCAAACACCTTTATATGAAGAGTTCCGCAGCACAATCAATGCAGCTAATGCTTTAGGAGCAGTTGGAACTGAGAATAGCAAGAGTGTATCTAATCTTCTGAAATTGCCTCCCAAATGTGGGTCACCAAGTAAGCTGCGTGGTAAAAGACTCTCATCAGTTGATGCTGCTAACTGTTCAAGCCGTCAGAGTCGTATAAAACAAGATAGCATATTGCAAGATCGAGCTCTGCAGGAAATTCAGTCGCCTCAGCTTGGGGAATGGAACAAATTGCTTCATACACAGCCAGAGTCTTGCACGCCAAGTTTCTCTGAGAGACAAAGGAAATGGAAAGAAGAGCTTGAAGAAGAACTTAAAAGGAAGCGAGAAATGATGCGGAGAGGAATTGTAGGAGCAATTCACTAA

Protein sequence

MQDFFGSVRRSLVFLAPDCDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVVKEDAPPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVKLLKNLSHPNIVRYLGTAREEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEAKDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKPMVDSRLNSNDLTNSTIQRSTGLKDICEMGSLRCSSVFSGNYGSRSNWGSSNFEDDMCQIDDKDLFVSSSIKYKSIISSHDLNKSFNPMCEPTDDWACKLDESSELGGNLMELSGIQTGQESTSKGNDFTFPCGQSAAEDDEEVTESKIKAFLDEKALELKKLQTPLYEEFRSTINAANALGAVGTENSKSVSNLLKLPPKCGSPSKLRGKRLSSVDAANCSSRQSRIKQDSILQDRALQEIQSPQLGEWNKLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGIVGAIH
Homology
BLAST of Lcy01g002090 vs. ExPASy Swiss-Prot
Match: Q40541 (Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097 GN=NPK1 PE=1 SV=1)

HSP 1 Score: 788.9 bits (2036), Expect = 4.4e-227
Identity = 422/666 (63.36%), Postives = 499/666 (74.92%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVF-LAPDCDDGGR-----FGGLVEKIGSSIRKSRNGLFSKHSVRALPP 60
           MQDF GSVRRSLVF  + D D G       FGG VEK+GSSIRKS  G+FSK  V ALP 
Sbjct: 1   MQDFIGSVRRSLVFKQSGDFDTGAAGVGSGFGGFVEKLGSSIRKSSIGIFSKAHVPALPS 60

Query: 61  VV---------KEDAPPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASR 120
           +          K+D PPI WRKGE+IGCGAFGRVYMGMN+DSGELLA+K+VSIA N ASR
Sbjct: 61  ISKAELPAKARKDDTPPIRWRKGEMIGCGAFGRVYMGMNVDSGELLAIKEVSIAMNGASR 120

Query: 121 EKAQAHIRELEEEVKLLKNLSHPNIVRYLGTAREEDSLNILLEFVPGGSISSLLGKFGSF 180
           E+AQAH+RELEEEV LLKNLSHPNIVRYLGTARE  SLNILLEFVPGGSISSLLGKFGSF
Sbjct: 121 ERAQAHVRELEEEVNLLKNLSHPNIVRYLGTAREAGSLNILLEFVPGGSISSLLGKFGSF 180

Query: 181 PESVIRTYTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATIN 240
           PESVIR YTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELAT+ 
Sbjct: 181 PESVIRMYTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATMT 240

Query: 241 GAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGN 300
           GAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCT+IEMATGKPPWSQQYQEVAALFHIG 
Sbjct: 241 GAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTIIEMATGKPPWSQQYQEVAALFHIGT 300

Query: 301 TKSHPPIPEHLSAEAKDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVES 360
           TKSHPPIPEHLSAE+KDFLLKCLQ+EP+ R +AS+LLQHPFV+ E+Q+    +R+S + +
Sbjct: 301 TKSHPPIPEHLSAESKDFLLKCLQKEPHLRHSASNLLQHPFVTAEHQEARPFLRSSFMGN 360

Query: 361 GKPMVDSRLNSNDLTNSTIQRS-TGLKDICEMGSLRCSSVFSGN-YGSRSNWGSSNFEDD 420
            + M   R++        ++ S  GLKD+C + ++RCS+V+  N  G  S W   N +DD
Sbjct: 361 PENMAAQRMDVRTSIIPDMRASCNGLKDVCGVSAVRCSTVYPENSLGKESLWKLGNSDDD 420

Query: 421 MCQIDDKDLFVSSSIKYKSIISSHDLNKSFNPMCEPTDDWACKLDESSELGGNLMELSGI 480
           MCQ+D+ D    +S+K  S + S    KSFNPMCEP +DW CK DES EL  +   L   
Sbjct: 421 MCQMDNDDFMFGASVKCSSDLHSPANYKSFNPMCEPDNDWPCKFDESPELTKSQANLHYD 480

Query: 481 QTGQESTS-------KGNDFTFPCGQSAAEDDEEVTESKIKAFLDEKALELKKLQTPLYE 540
           Q   + T+       +   FTFP GQSAAEDD+E+TESKI+AFLDEKA++LKKLQTPLYE
Sbjct: 481 QATIKPTNNPIMSYKEDLAFTFPSGQSAAEDDDELTESKIRAFLDEKAMDLKKLQTPLYE 540

Query: 541 EFRSTINAANALGAVGTENSKSVSNLLKLPPKCGSPSKLRGKRLSSVDAANCS----SRQ 600
            F +++N ++    VGT N ++V + + LPPK  SP ++  +RLS+     C+    +  
Sbjct: 541 GFYNSLNVSSTPSPVGTGNKENVPSNINLPPKSRSPKRMLSRRLSTAIEGACAPSPVTHS 600

Query: 601 SRIKQDSILQDRALQEIQSPQLGEWNKLLHTQPESCTPSFSERQRKWKEELEEELKRKRE 639
            RI     L   A+QE Q P+  EW  LL +Q E+   SFSERQR+WKEEL+EEL+RKRE
Sbjct: 601 KRISNIGGLNGEAIQEAQLPRHNEWKDLLGSQREAVNSSFSERQRRWKEELDEELQRKRE 660

BLAST of Lcy01g002090 vs. ExPASy Swiss-Prot
Match: O22042 (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=ANP3 PE=1 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 1.3e-199
Identity = 395/654 (60.40%), Postives = 461/654 (70.49%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVF----LAPDCDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVV 60
           MQD  GSVRRSLVF       D   GG   G V KI SSIR SR GLFSK     LP   
Sbjct: 1   MQDILGSVRRSLVFRSSLAGDDGTSGGGLSGFVGKINSSIRSSRIGLFSK-PPPGLPAPR 60

Query: 61  KEDAPPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELE 120
           KE+AP I WRKGELIGCGAFGRVYMGMNLDSGELLA+KQV IA +SAS+EK Q HIRELE
Sbjct: 61  KEEAPSIRWRKGELIGCGAFGRVYMGMNLDSGELLAIKQVLIAPSSASKEKTQGHIRELE 120

Query: 121 EEVKLLKNLSHPNIVRYLGTAREEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQ 180
           EEV+LLKNLSHPNIVRYLGT RE DSLNIL+EFVPGGSISSLL KFGSFPE VI  YTKQ
Sbjct: 121 EEVQLLKNLSHPNIVRYLGTVRESDSLNILMEFVPGGSISSLLEKFGSFPEPVIIMYTKQ 180

Query: 181 LLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYW 240
           LLLGLEYLH NGIMHRDIKGANILVDNKGCI+LADFGASKKVVELAT+NGAKSMKGTPYW
Sbjct: 181 LLLGLEYLHNNGIMHRDIKGANILVDNKGCIRLADFGASKKVVELATVNGAKSMKGTPYW 240

Query: 241 MAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHL 300
           MAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWS+QYQ+ AA+ HIG TK+HPPIPE L
Sbjct: 241 MAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIGRTKAHPPIPEDL 300

Query: 301 SAEAKDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKPMVDSRLNS 360
           S EAKDFL+KCL +EP+ R +A++LLQHPFV+ + Q+P+ A R S  E G P+    +N 
Sbjct: 301 SPEAKDFLMKCLHKEPSLRLSATELLQHPFVTGKRQEPYPAYRNSLTECGNPITTQGMNV 360

Query: 361 NDLTNSTIQRST--GLKDICEMGSLRCSSVF---SGNYGSRSNWGSSNFEDDMCQIDDKD 420
               NS I+RST  GLKD+CE+GSLR S ++   S N G     G S   DD+CQ D  D
Sbjct: 361 RSSINSLIRRSTCSGLKDVCELGSLRSSIIYPQKSNNSGFGWRDGDS---DDLCQTDMDD 420

Query: 421 LFVSSSIKYKSIISSHDLNKSFNPMCEPTDDWACKLDESSELGGNLMELSGIQTGQEST- 480
           L    S++   +  S DLNKSFNPMC+ TD+W+CK DES ++  +   L   Q  Q  T 
Sbjct: 421 LCNIESVRNNVLSQSTDLNKSFNPMCDSTDNWSCKFDESPKVMKSKSNLLSYQASQLQTG 480

Query: 481 ---SKGNDFTFPCGQSAAEDDEEVTESKIKAFLDEKALELKKLQTPLYEEFRSTINAANA 540
               +    TF  G S AEDD + TE KIK+FLDEKA +LK+LQTPL EEF + +N    
Sbjct: 481 VPCDEETSLTFAGGSSVAEDDYKGTELKIKSFLDEKAQDLKRLQTPLLEEFHNAMNPGIP 540

Query: 541 LGAVGTENSKSVSNLLKLPPKCGSPSKLRGKRLSSVDAANCSSRQSRIKQDSILQDRALQ 600
            GA+G  N   + NL  LP    +P +L  +RLS++  A  S  +S   + ++   R +Q
Sbjct: 541 QGALGDTN---IYNLPNLPSISKTPKRLPSRRLSAISDAMPSPLKS--SKRTLNTSRVMQ 600

Query: 601 EIQSP-QLGEWNKLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGIVG 641
               P Q+ E  K    +  + +  FSE +RKW+EEL EEL+R RE +R    G
Sbjct: 601 SGTEPTQVNESTK----KGVNNSRCFSEIRRKWEEELYEELERHRENLRHAGAG 641

BLAST of Lcy01g002090 vs. ExPASy Swiss-Prot
Match: O22040 (Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana OX=3702 GN=ANP1 PE=1 SV=2)

HSP 1 Score: 613.2 bits (1580), Expect = 3.3e-174
Identity = 373/671 (55.59%), Postives = 448/671 (66.77%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLAPDCDDGGRF------GGLVEKIGSSIRKSRNGLFSKHSVRALPP 60
           MQDFFGSVRRSLVF  P  DD  +       G L +KI S IRKS+  +F K S    PP
Sbjct: 1   MQDFFGSVRRSLVF-RPSSDDDNQENQPPFPGVLADKITSCIRKSK--IFIKPSFSPPPP 60

Query: 61  VVKED-APPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIR 120
               D APPI WRKG+LIG GAFG VYMGMNLDSGELLAVKQV IAAN AS+EK QAHI+
Sbjct: 61  ANTVDMAPPISWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQ 120

Query: 121 ELEEEVKLLKNLSHPNIVRYLGTAREEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTY 180
           ELEEEVKLLKNLSHPNIVRYLGT RE+D+LNILLEFVPGGSISSLL KFG FPESV+RTY
Sbjct: 121 ELEEEVKLLKNLSHPNIVRYLGTVREDDTLNILLEFVPGGSISSLLEKFGPFPESVVRTY 180

Query: 181 TKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGT 240
           T+QLLLGLEYLH + IMHRDIKGANILVDNKGCIKLADFGASK+V ELAT+ GAKSMKGT
Sbjct: 181 TRQLLLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMTGAKSMKGT 240

Query: 241 PYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIP 300
           PYWMAPEVILQTGHSFSADIWSVGCTVIEM TGK PWSQQY+EVAA+F IG TKSHPPIP
Sbjct: 241 PYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIP 300

Query: 301 EHLSAEAKDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKPMVDSR 360
           + LS++AKDFLLKCLQE PN RPTAS+LL+HPFV  ++++  +    S + +    +  +
Sbjct: 301 DTLSSDAKDFLLKCLQEVPNLRPTASELLKHPFVMGKHKESASTDLGSVLNNLSTPLPLQ 360

Query: 361 LNSNDLTNSTIQRSTGLKDICEMGSLRCS------SVFSGNYGSRSNWGSSNFEDDMCQI 420
           +N+   T  +     G  D+C  GSL  S      S+ + N   +++ G    EDDMC I
Sbjct: 361 INNTKSTPDSTCDDVG--DMCNFGSLNYSLVDPVKSIQNKNLWQQNDNGGD--EDDMCLI 420

Query: 421 DDKDLFVSSSIKYKSIISSHDLNKSFNPMCEPTDDWACKLDESSELGGNLMELSGIQTGQ 480
           DD++          ++     L KS + + + +     K DES   G             
Sbjct: 421 DDENFLTFDGEMSSTLEKDCHLKKSCDDISDMSIALKSKFDESPGNG------------- 480

Query: 481 ESTSKGNDFTFPCGQ-SAAEDDEEVTESKIKAFLDEKALELKKLQTPLYEEF-RSTINAA 540
               K +  +  C Q S +EDD+E+TESKIKAFLDEKA +LKKLQTPLYEEF  S I  +
Sbjct: 481 ---EKESTMSMECDQPSYSEDDDELTESKIKAFLDEKAADLKKLQTPLYEEFYNSLITFS 540

Query: 541 NALGAVGTENSK---SVSNLLKLPPKCGSPSK--LRGKRLSSVDAANCS-------SRQS 600
            +       NSK   +    LKLPPK  SPS+  L G    + DA +CS       SR+ 
Sbjct: 541 PSCMESNLSNSKREDTARGFLKLPPKSRSPSRGPLGGSPSRATDATSCSKSPGSGGSREL 600

Query: 601 RIKQDSILQDRALQEIQSPQLGEWNKLL--HTQPESCTPSFSERQRKWKEELEEELKRKR 642
            I       D A Q+  S ++ +W  L+    Q  S   + SE ++KWKEEL++EL+RKR
Sbjct: 601 NINNGG---DEASQDGVSARVTDWRGLVVDTKQELSQCVALSEIEKKWKEELDQELERKR 645

BLAST of Lcy01g002090 vs. ExPASy Swiss-Prot
Match: Q9FZ36 (Mitogen-activated protein kinase kinase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=ANP2 PE=2 SV=1)

HSP 1 Score: 595.9 bits (1535), Expect = 5.5e-169
Identity = 354/656 (53.96%), Postives = 442/656 (67.38%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLAPDCDDGGR-----FGGLV-EKIGSSIRKSRNGLFSKHSVRALPP 60
           MQD FGSVRRSLVF +   D+        F  L+ +KI S IRKS   +F+K      PP
Sbjct: 1   MQDLFGSVRRSLVFRSTTDDENQENHPPPFPSLLADKITSCIRKSM--VFAKSQ---SPP 60

Query: 61  --VVKEDAPPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHI 120
                +  PPI WRKG+LIG GAFG VYMGMNLDSGELLAVKQV I +N AS+EK QAHI
Sbjct: 61  NNSTVQIKPPIRWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLITSNCASKEKTQAHI 120

Query: 121 RELEEEVKLLKNLSHPNIVRYLGTAREEDSLNILLEFVPGGSISSLLGKFGSFPESVIRT 180
           +ELEEEVKLLKNLSHPNIVRYLGT RE+++LNILLEFVPGGSISSLL KFG+FPESV+RT
Sbjct: 121 QELEEEVKLLKNLSHPNIVRYLGTVREDETLNILLEFVPGGSISSLLEKFGAFPESVVRT 180

Query: 181 YTKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKG 240
           YT QLLLGLEYLH + IMHRDIKGANILVDN+GCIKLADFGASK+V ELATI+GAKSMKG
Sbjct: 181 YTNQLLLGLEYLHNHAIMHRDIKGANILVDNQGCIKLADFGASKQVAELATISGAKSMKG 240

Query: 241 TPYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPI 300
           TPYWMAPEVILQTGHSFSADIWSVGCTVIEM TGK PWSQQY+E+AA+FHIG TKSHPPI
Sbjct: 241 TPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTKSHPPI 300

Query: 301 PEHLSAEAKDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKPMVDS 360
           P+++S++A DFLLKCLQ+EPN RPTAS+LL+HPFV+ + ++  +    S +++    + S
Sbjct: 301 PDNISSDANDFLLKCLQQEPNLRPTASELLKHPFVTGKQKESASKDLTSFMDNSCSPLPS 360

Query: 361 RLNSNDLTNSTIQRSTGLKDICEMGSLRCS------SVFSGNYGSRSNWGSSNFED-DMC 420
            L   ++T+     S  + DIC +GSL C+      S+ + +   +SN G  + +D DMC
Sbjct: 361 EL--TNITSYQTSTSDDVGDICNLGSLTCTLAFPEKSIQNNSLCLKSNNGYDDDDDNDMC 420

Query: 421 QIDDKDLFVSSSIKYKSIISSHDLNKSFNPMCEPTDDWACKLDESSELGGNLMELSGIQT 480
            IDD++    +     S+ ++ D  KS + M E +D   CK DE+S   GN         
Sbjct: 421 LIDDENFLTYNGETGPSLDNNTDAKKSCDTMSEISDILKCKFDENS---GN--------- 480

Query: 481 GQESTSKGNDFTFPCGQSAAEDDEEVTESKIKAFLDEKALELKKLQTPLYEEFRSTINAA 540
           G+  T    +   P   S +ED+ E+TESKIKAFLD+KA ELKKLQTPLYEEF + +   
Sbjct: 481 GETETKVSMEVDHP---SYSEDENELTESKIKAFLDDKAAELKKLQTPLYEEFYNGMITC 540

Query: 541 NALGAVGTENS----KSVSNLLKLPPKCGSPSKLR-GKRLSSVDAANCSSRQSRIKQDSI 600
           + +      N+    ++    LKLPPK  SPS+   G+  S    A C S+       S 
Sbjct: 541 SPICMESNINNNKREEAPRGFLKLPPKSRSPSQGHIGRSPSRATDAACCSKSPESGNSS- 600

Query: 601 LQDRALQEIQSPQLGEWNKLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRR 637
                     +P+    +     +  S + + SE +RKWKEEL++EL+RKR  + R
Sbjct: 601 ---------GAPKNSNASAGAEQESNSQSVALSEIERKWKEELDQELERKRREITR 624

BLAST of Lcy01g002090 vs. ExPASy Swiss-Prot
Match: Q54R82 (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=44689 GN=mkkA PE=1 SV=2)

HSP 1 Score: 306.2 bits (783), Expect = 8.6e-82
Identity = 142/265 (53.58%), Postives = 196/265 (73.96%), Query Frame = 0

Query: 63  IWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVKLL 122
           I W+KG+++G G +G VY+G+N D+GEL AVKQ+ I  +  S  K +  I    +E++++
Sbjct: 168 IKWQKGQILGRGGYGSVYLGLNKDTGELFAVKQLEI-VDINSDPKLKNMILSFSKEIEVM 227

Query: 123 KNLSHPNIVRYLGTAREEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLGLE 182
           ++L H NIVRYLGT+ ++  L++ LE++PGGSISSLLGKFG+F E+VI+ YTKQ+L GL 
Sbjct: 228 RSLRHDNIVRYLGTSLDQSFLSVFLEYIPGGSISSLLGKFGAFSENVIKVYTKQILQGLS 287

Query: 183 YLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPEVI 242
           +LH N I+HRDIKGANIL+D KG +KL+DFG SK    +  ++  KSM+GTPYWMAPEVI
Sbjct: 288 FLHANSIIHRDIKGANILIDTKGIVKLSDFGCSKSFSGI--VSQFKSMQGTPYWMAPEVI 347

Query: 243 LQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEAKD 302
            QTGH  S+DIWS+GC ++EMAT +PPWS   +  A ++HI ++ S P IP H+S EA D
Sbjct: 348 KQTGHGRSSDIWSLGCVIVEMATAQPPWSNITELAAVMYHIASSNSIPNIPSHMSQEAFD 407

Query: 303 FLLKCLQEEPNSRPTASDLLQHPFV 328
           FL  C + +P  RP A+ LL+HPF+
Sbjct: 408 FLNLCFKRDPKERPDANQLLKHPFI 429

BLAST of Lcy01g002090 vs. ExPASy TrEMBL
Match: A0A6J1KXU2 (mitogen-activated protein kinase kinase kinase NPK1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111499179 PE=4 SV=1)

HSP 1 Score: 1213.4 bits (3138), Expect = 0.0e+00
Identity = 607/645 (94.11%), Postives = 625/645 (96.90%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLAPDCDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVVKEDA 60
           MQDFFGSVRRSLVFLAPD DDGG+FGGLVE+IGSSIRKSRNGLFSKHSVRALPPV KEDA
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           PPIWWRKGELIGCGAFGRVYMGMNLDSGEL+A+KQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTAREEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGTAREE SLNILLEFVPGGSISSLLGKFGSFPESV++TYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKPMVDSRLNSNDLT 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQ+PHAAVR SS+ESGK M DSRLNS D+T
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAAVRVSSLESGKQMADSRLNSKDIT 360

Query: 361 NSTIQRST--GLKDICEMGSLRCSSVFSGNYGSRSNWGSSNFEDDMCQIDDKDLFVSSSI 420
            S IQRST  GLKDICEMGSLRCSSVFSGNY  RSNWGSSNFEDDMCQIDDK+LF SSSI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKNLFASSSI 420

Query: 421 KYKSIISSHDLNKSFNPMCEPTDDWACKLDESSELGGNLMELSGIQTGQESTSKGNDFTF 480
           KYKSIISS+DLNKSFNPMCEPTDDWACKLDESSEL GNLMELSGI+TGQESTSKGNDFTF
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDDWACKLDESSELRGNLMELSGIRTGQESTSKGNDFTF 480

Query: 481 PCGQSAAEDDEEVTESKIKAFLDEKALELKKLQTPLYEEFRSTINAANALGAVGTENSKS 540
           PCGQS AEDDEEVT+SKI+AFLDEKALELKKLQTPLYEEFRST+NAANALGAVGTENSKS
Sbjct: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAVGTENSKS 540

Query: 541 VSNLLKLPPKCGSPSKLRGKRLSSVDAANCSSRQSRIKQDSILQDRALQEIQSPQLGEWN 600
           VSN LKLPPKCGSPSKLRGKRLSS+DA NCSSRQSRIKQ SILQDRALQEIQSPQ+GEWN
Sbjct: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWN 600

Query: 601 KLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGIVGAIH 644
           KLLHTQPESCTP+FSERQRKWKEELEEELKRKREMMRRG VGAIH
Sbjct: 601 KLLHTQPESCTPTFSERQRKWKEELEEELKRKREMMRRGTVGAIH 645

BLAST of Lcy01g002090 vs. ExPASy TrEMBL
Match: A0A6J1H661 (mitogen-activated protein kinase kinase kinase NPK1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111459961 PE=4 SV=1)

HSP 1 Score: 1209.5 bits (3128), Expect = 0.0e+00
Identity = 606/645 (93.95%), Postives = 623/645 (96.59%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLAPDCDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVVKEDA 60
           MQDFFGSVRRSLVFLAPD DDGG+FGGLVE+IGSSIRKSRNGLFSKHSVRALPPV KEDA
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           PPIWWRKGELIGCGAFGRVYMGMNLDSGEL+A+KQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTAREEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGTAREE SLNILLEFVPGGSISSLLGKFGSFPESV++TYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKPMVDSRLNSNDLT 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQ+PHAAVRASS+ESGK M  +RLNSND+T
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAAVRASSLESGKQMAGARLNSNDIT 360

Query: 361 NSTIQRST--GLKDICEMGSLRCSSVFSGNYGSRSNWGSSNFEDDMCQIDDKDLFVSSSI 420
            S IQRST  GLKDICEMGSLRCSSVFSGNY  RSNWGSSN EDDMCQIDDKDLF SSSI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNSEDDMCQIDDKDLFASSSI 420

Query: 421 KYKSIISSHDLNKSFNPMCEPTDDWACKLDESSELGGNLMELSGIQTGQESTSKGNDFTF 480
           KYKSIISS+DLNKSFNPMCEPTDDWACKLDESSEL GNLMELSGIQTGQESTSKGNDFTF
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDDWACKLDESSELRGNLMELSGIQTGQESTSKGNDFTF 480

Query: 481 PCGQSAAEDDEEVTESKIKAFLDEKALELKKLQTPLYEEFRSTINAANALGAVGTENSKS 540
           PCGQS AEDDEEVT+SKI+AFLDEKALELKKLQTPLYEEFRST+NAANALGA GTENSKS
Sbjct: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSKS 540

Query: 541 VSNLLKLPPKCGSPSKLRGKRLSSVDAANCSSRQSRIKQDSILQDRALQEIQSPQLGEWN 600
           VSN LKLPPKCGSPSKLRGKRLSS+D  NCSSRQSR+KQ SILQDRALQEIQSPQ+GEWN
Sbjct: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDDGNCSSRQSRVKQASILQDRALQEIQSPQIGEWN 600

Query: 601 KLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGIVGAIH 644
           KLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRG VGAIH
Sbjct: 601 KLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGTVGAIH 645

BLAST of Lcy01g002090 vs. ExPASy TrEMBL
Match: A0A6J1L4Z4 (mitogen-activated protein kinase kinase kinase NPK1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499179 PE=4 SV=1)

HSP 1 Score: 1208.7 bits (3126), Expect = 0.0e+00
Identity = 607/646 (93.96%), Postives = 625/646 (96.75%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLAPDCDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVVKEDA 60
           MQDFFGSVRRSLVFLAPD DDGG+FGGLVE+IGSSIRKSRNGLFSKHSVRALPPV KEDA
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           PPIWWRKGELIGCGAFGRVYMGMNLDSGEL+A+KQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTAREEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGTAREE SLNILLEFVPGGSISSLLGKFGSFPESV++TYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKPMVDSRLNSNDLT 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQ+PHAAVR SS+ESGK M DSRLNS D+T
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAAVRVSSLESGKQMADSRLNSKDIT 360

Query: 361 NSTIQRST--GLKDICEMGSLRCSSVFSGNYGSRSNWGSSNFEDDMCQIDDKDLFVSSSI 420
            S IQRST  GLKDICEMGSLRCSSVFSGNY  RSNWGSSNFEDDMCQIDDK+LF SSSI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKNLFASSSI 420

Query: 421 KYKSIISSHDLNKSFNPMCEPTDDWACKLDESSELGGNLMELSGIQTGQESTSKGNDFTF 480
           KYKSIISS+DLNKSFNPMCEPTDDWACKLDESSEL GNLMELSGI+TGQESTSKGNDFTF
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDDWACKLDESSELRGNLMELSGIRTGQESTSKGNDFTF 480

Query: 481 PCGQSAAEDDEEVTESKIKAFLDEKALELKKLQTPLYEEFRSTINAANALGAVGTENSKS 540
           PCGQS AEDDEEVT+SKI+AFLDEKALELKKLQTPLYEEFRST+NAANALGAVGTENSKS
Sbjct: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAVGTENSKS 540

Query: 541 VSNLLKLPPKCGSPSKLRGKRLSSVDAANCSSRQSRIKQDSILQDRALQEIQSPQLGEWN 600
           VSN LKLPPKCGSPSKLRGKRLSS+DA NCSSRQSRIKQ SILQDRALQEIQSPQ+GEWN
Sbjct: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWN 600

Query: 601 KLLHTQPESCTPS-FSERQRKWKEELEEELKRKREMMRRGIVGAIH 644
           KLLHTQPESCTP+ FSERQRKWKEELEEELKRKREMMRRG VGAIH
Sbjct: 601 KLLHTQPESCTPTFFSERQRKWKEELEEELKRKREMMRRGTVGAIH 646

BLAST of Lcy01g002090 vs. ExPASy TrEMBL
Match: A0A6J1H434 (mitogen-activated protein kinase kinase kinase NPK1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459961 PE=4 SV=1)

HSP 1 Score: 1203.0 bits (3111), Expect = 0.0e+00
Identity = 605/646 (93.65%), Postives = 622/646 (96.28%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLAPDCDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVVKEDA 60
           MQDFFGSVRRSLVFLAPD DDGG+FGGLVE+IGSSIRKSRNGLFSKHSVRALPPV KEDA
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           PPIWWRKGELIGCGAFGRVYMGMNLDSGEL+A+KQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTAREEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGTAREE SLNILLEFVPGGSISSLLGKFGSFPESV++TYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKPMVDSRLNSNDLT 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQ+PHAAVRASS+ESGK M  +RLNSND+T
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAAVRASSLESGKQMAGARLNSNDIT 360

Query: 361 NSTIQRST--GLKDICEMGSLRCSSVFSGNYGSRSNWGSSNFEDDMCQIDDKDLFVSSSI 420
            S IQRST  GLKDICEMGSLRCSSVFSGNY  RSNWGSSN EDDMCQIDDKDLF SSSI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNSEDDMCQIDDKDLFASSSI 420

Query: 421 KYKSIISSHDLNKSFNPMCEPTDDWACKLDESSELGGNLMELSGIQTGQESTSKGNDFTF 480
           KYKSIISS+DLNKSFNPMCEPTDDWACKLDESSEL GNLMELSGIQTGQESTSKGNDFTF
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDDWACKLDESSELRGNLMELSGIQTGQESTSKGNDFTF 480

Query: 481 PCGQSAAEDDEEVTESKIKAFLDEKALELKKLQTPLYEEFRSTINAANALGAVGTENSKS 540
           PCGQS AEDDEEVT+SKI+AFLDEKALELKKLQTPLYEEFRST+NAANALGA GTENSKS
Sbjct: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSKS 540

Query: 541 VSNLLKLPPKCGSPSKLRGKRLSSVDAANCSSRQSRIKQDSILQDRALQEIQSPQLGEWN 600
           VSN LKLPPKCGSPSKLRGKRLSS+D  NCSSRQSR+KQ SILQDRALQEIQSPQ+GEWN
Sbjct: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDDGNCSSRQSRVKQASILQDRALQEIQSPQIGEWN 600

Query: 601 KLLHTQPESCTPS-FSERQRKWKEELEEELKRKREMMRRGIVGAIH 644
           KLLHTQPESCTP  FSERQRKWKEELEEELKRKREMMRRG VGAIH
Sbjct: 601 KLLHTQPESCTPRFFSERQRKWKEELEEELKRKREMMRRGTVGAIH 646

BLAST of Lcy01g002090 vs. ExPASy TrEMBL
Match: A0A6J1CE75 (mitogen-activated protein kinase kinase kinase NPK1 OS=Momordica charantia OX=3673 GN=LOC111009894 PE=4 SV=1)

HSP 1 Score: 1193.7 bits (3087), Expect = 0.0e+00
Identity = 605/642 (94.24%), Postives = 618/642 (96.26%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLAPDCDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVVKEDA 60
           MQDFFGSVRRSLVFL PD DDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPV+KEDA
Sbjct: 1   MQDFFGSVRRSLVFLTPDGDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVMKEDA 60

Query: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           PPIWWR+GELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRRGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTAREEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGT R EDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTTRGEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSF ADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFPADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKPMVDSRLNSNDLT 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGK MV S LNSNDL 
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKQMVGSGLNSNDLA 360

Query: 361 NSTIQRST--GLKDICEMGSLRCSSVFSGNYGSRSNWGSSNFEDDMCQIDDKDLFVSSSI 420
           NSTIQRST  GLKDICEMGSLRCSSV+SGNY S+SNWGSSNFE+DMCQIDDKDL VS SI
Sbjct: 361 NSTIQRSTCVGLKDICEMGSLRCSSVYSGNYTSQSNWGSSNFEEDMCQIDDKDLLVSPSI 420

Query: 421 KYKSIISSHDLNKSFNPMCEPT-DDWACKLDESSELGGNLMELSGIQTGQESTSKGNDFT 480
           KYKSIISS DLNKSFNPMCEPT DDWACKLD SSELGGNLM+LSGIQT QE+T K NDFT
Sbjct: 421 KYKSIISSSDLNKSFNPMCEPTDDDWACKLDGSSELGGNLMDLSGIQTSQETTCKENDFT 480

Query: 481 FPCGQSAAEDDEEVTESKIKAFLDEKALELKKLQTPLYEEFRSTINAANALGAVGTENSK 540
           FPCGQSA EDDEEVTESKI+AFLDEKA+ELKK+QTPLYEEFRSTINAANALGAVGTENSK
Sbjct: 481 FPCGQSAVEDDEEVTESKIRAFLDEKAIELKKMQTPLYEEFRSTINAANALGAVGTENSK 540

Query: 541 SVSNLLKLPPKCGSPSKLRGKRLSSVDAANCSSRQSRIKQDSILQDRALQEIQSPQLGEW 600
           SVSN LKLPPKCGSPSKLRGKRLSS+DA+NCSSRQSRIKQ SIL+DRALQEIQSPQLGEW
Sbjct: 541 SVSNFLKLPPKCGSPSKLRGKRLSSIDASNCSSRQSRIKQASILEDRALQEIQSPQLGEW 600

Query: 601 NKLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGIV 640
           NKLLHTQPES TPSFSERQRKWKEELEEELKRKREMMRRGIV
Sbjct: 601 NKLLHTQPESSTPSFSERQRKWKEELEEELKRKREMMRRGIV 642

BLAST of Lcy01g002090 vs. NCBI nr
Match: XP_023547660.1 (mitogen-activated protein kinase kinase kinase NPK1 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023547661.1 mitogen-activated protein kinase kinase kinase NPK1 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023547662.1 mitogen-activated protein kinase kinase kinase NPK1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1219.1 bits (3153), Expect = 0.0e+00
Identity = 611/645 (94.73%), Postives = 626/645 (97.05%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLAPDCDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVVKEDA 60
           MQDFFGSVRRSLVFLAPD DDGG+FGGLVE+IGSSIRKSRNGLFSKHSVRALPPV KEDA
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           PPIWWRKGELIGCGAFGRVYMGMNLDSGEL+A+KQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTAREEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGTAREE SLNILLEFVPGGSISSLLGKFGSFPESV++TYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKPMVDSRLNSNDLT 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQ+PHAAVRASS+ESGK M DSRLNSND+T
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAAVRASSLESGKQMADSRLNSNDIT 360

Query: 361 NSTIQRST--GLKDICEMGSLRCSSVFSGNYGSRSNWGSSNFEDDMCQIDDKDLFVSSSI 420
            S IQRST  GLKDICEMGSLRCSSVFSGNY  RSNWGSSNFEDDMCQIDDKDLF SSSI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKDLFASSSI 420

Query: 421 KYKSIISSHDLNKSFNPMCEPTDDWACKLDESSELGGNLMELSGIQTGQESTSKGNDFTF 480
           KYKSIISS+DLNKSFNPMCEPTDDWACKLDESSEL GNLMELSGIQTGQESTSKGNDFTF
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDDWACKLDESSELRGNLMELSGIQTGQESTSKGNDFTF 480

Query: 481 PCGQSAAEDDEEVTESKIKAFLDEKALELKKLQTPLYEEFRSTINAANALGAVGTENSKS 540
           PCGQS AEDDEEVT+SKI+AFLDEKALELKKLQTPLYEEFRST+NAANALGA GTENSKS
Sbjct: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSKS 540

Query: 541 VSNLLKLPPKCGSPSKLRGKRLSSVDAANCSSRQSRIKQDSILQDRALQEIQSPQLGEWN 600
           VSN LKLPPKCGSPSKLRGKRLSS+DA NCSSRQSRIKQ SILQDRALQEIQSPQ+GEWN
Sbjct: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWN 600

Query: 601 KLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGIVGAIH 644
           KLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRG VGAIH
Sbjct: 601 KLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGTVGAIH 645

BLAST of Lcy01g002090 vs. NCBI nr
Match: XP_023006461.1 (mitogen-activated protein kinase kinase kinase NPK1 isoform X2 [Cucurbita maxima])

HSP 1 Score: 1213.4 bits (3138), Expect = 0.0e+00
Identity = 607/645 (94.11%), Postives = 625/645 (96.90%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLAPDCDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVVKEDA 60
           MQDFFGSVRRSLVFLAPD DDGG+FGGLVE+IGSSIRKSRNGLFSKHSVRALPPV KEDA
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           PPIWWRKGELIGCGAFGRVYMGMNLDSGEL+A+KQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTAREEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGTAREE SLNILLEFVPGGSISSLLGKFGSFPESV++TYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKPMVDSRLNSNDLT 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQ+PHAAVR SS+ESGK M DSRLNS D+T
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAAVRVSSLESGKQMADSRLNSKDIT 360

Query: 361 NSTIQRST--GLKDICEMGSLRCSSVFSGNYGSRSNWGSSNFEDDMCQIDDKDLFVSSSI 420
            S IQRST  GLKDICEMGSLRCSSVFSGNY  RSNWGSSNFEDDMCQIDDK+LF SSSI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKNLFASSSI 420

Query: 421 KYKSIISSHDLNKSFNPMCEPTDDWACKLDESSELGGNLMELSGIQTGQESTSKGNDFTF 480
           KYKSIISS+DLNKSFNPMCEPTDDWACKLDESSEL GNLMELSGI+TGQESTSKGNDFTF
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDDWACKLDESSELRGNLMELSGIRTGQESTSKGNDFTF 480

Query: 481 PCGQSAAEDDEEVTESKIKAFLDEKALELKKLQTPLYEEFRSTINAANALGAVGTENSKS 540
           PCGQS AEDDEEVT+SKI+AFLDEKALELKKLQTPLYEEFRST+NAANALGAVGTENSKS
Sbjct: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAVGTENSKS 540

Query: 541 VSNLLKLPPKCGSPSKLRGKRLSSVDAANCSSRQSRIKQDSILQDRALQEIQSPQLGEWN 600
           VSN LKLPPKCGSPSKLRGKRLSS+DA NCSSRQSRIKQ SILQDRALQEIQSPQ+GEWN
Sbjct: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWN 600

Query: 601 KLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGIVGAIH 644
           KLLHTQPESCTP+FSERQRKWKEELEEELKRKREMMRRG VGAIH
Sbjct: 601 KLLHTQPESCTPTFSERQRKWKEELEEELKRKREMMRRGTVGAIH 645

BLAST of Lcy01g002090 vs. NCBI nr
Match: KAG6575002.1 (Mitogen-activated protein kinase kinase kinase NPK1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013573.1 Mitogen-activated protein kinase kinase kinase NPK1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1211.1 bits (3132), Expect = 0.0e+00
Identity = 607/645 (94.11%), Postives = 623/645 (96.59%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLAPDCDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVVKEDA 60
           MQDFFGSVRRSLVFLAPD DDGG+FGGLVE+IGSSIRKSRNGLFSKHSVRALPPV KEDA
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           P IWWRKGELIGCGAFGRVYMGMNLDSGEL+A+KQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PSIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTAREEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGTAREE SLNILLEFVPGGSISSLLGKFGSFPESV++TYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKPMVDSRLNSNDLT 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQ+PHA VRASS+ESGK M DSRLNSND+T
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAPVRASSLESGKQMADSRLNSNDIT 360

Query: 361 NSTIQRST--GLKDICEMGSLRCSSVFSGNYGSRSNWGSSNFEDDMCQIDDKDLFVSSSI 420
            S IQRST  GLKDICEMGSLRCSSVFSGNY  RSNWGSSNFEDDMCQIDDKDLF SSSI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKDLFASSSI 420

Query: 421 KYKSIISSHDLNKSFNPMCEPTDDWACKLDESSELGGNLMELSGIQTGQESTSKGNDFTF 480
           KYKSIISS+DLNKSFNPMCEPTDDW CKLDESSEL GNLMELSGIQTGQESTSKGNDFTF
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDDWPCKLDESSELRGNLMELSGIQTGQESTSKGNDFTF 480

Query: 481 PCGQSAAEDDEEVTESKIKAFLDEKALELKKLQTPLYEEFRSTINAANALGAVGTENSKS 540
           PCGQS AEDDEEVT+SKI+AFLDEKALELKKLQTPLYEEFRST+NAANALGA GTENSKS
Sbjct: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSKS 540

Query: 541 VSNLLKLPPKCGSPSKLRGKRLSSVDAANCSSRQSRIKQDSILQDRALQEIQSPQLGEWN 600
           VSN LKLPPKCGSPSKLRGKRLSS+DA NCSSRQSRIKQ SILQDRALQEIQSPQ+GEWN
Sbjct: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWN 600

Query: 601 KLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGIVGAIH 644
           +LLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRG VGAIH
Sbjct: 601 RLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGTVGAIH 645

BLAST of Lcy01g002090 vs. NCBI nr
Match: XP_022958799.1 (mitogen-activated protein kinase kinase kinase NPK1 isoform X2 [Cucurbita moschata])

HSP 1 Score: 1209.5 bits (3128), Expect = 0.0e+00
Identity = 606/645 (93.95%), Postives = 623/645 (96.59%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLAPDCDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVVKEDA 60
           MQDFFGSVRRSLVFLAPD DDGG+FGGLVE+IGSSIRKSRNGLFSKHSVRALPPV KEDA
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           PPIWWRKGELIGCGAFGRVYMGMNLDSGEL+A+KQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTAREEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGTAREE SLNILLEFVPGGSISSLLGKFGSFPESV++TYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKPMVDSRLNSNDLT 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQ+PHAAVRASS+ESGK M  +RLNSND+T
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAAVRASSLESGKQMAGARLNSNDIT 360

Query: 361 NSTIQRST--GLKDICEMGSLRCSSVFSGNYGSRSNWGSSNFEDDMCQIDDKDLFVSSSI 420
            S IQRST  GLKDICEMGSLRCSSVFSGNY  RSNWGSSN EDDMCQIDDKDLF SSSI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNSEDDMCQIDDKDLFASSSI 420

Query: 421 KYKSIISSHDLNKSFNPMCEPTDDWACKLDESSELGGNLMELSGIQTGQESTSKGNDFTF 480
           KYKSIISS+DLNKSFNPMCEPTDDWACKLDESSEL GNLMELSGIQTGQESTSKGNDFTF
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDDWACKLDESSELRGNLMELSGIQTGQESTSKGNDFTF 480

Query: 481 PCGQSAAEDDEEVTESKIKAFLDEKALELKKLQTPLYEEFRSTINAANALGAVGTENSKS 540
           PCGQS AEDDEEVT+SKI+AFLDEKALELKKLQTPLYEEFRST+NAANALGA GTENSKS
Sbjct: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAGGTENSKS 540

Query: 541 VSNLLKLPPKCGSPSKLRGKRLSSVDAANCSSRQSRIKQDSILQDRALQEIQSPQLGEWN 600
           VSN LKLPPKCGSPSKLRGKRLSS+D  NCSSRQSR+KQ SILQDRALQEIQSPQ+GEWN
Sbjct: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDDGNCSSRQSRVKQASILQDRALQEIQSPQIGEWN 600

Query: 601 KLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGIVGAIH 644
           KLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRG VGAIH
Sbjct: 601 KLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGTVGAIH 645

BLAST of Lcy01g002090 vs. NCBI nr
Match: XP_023006458.1 (mitogen-activated protein kinase kinase kinase NPK1 isoform X1 [Cucurbita maxima] >XP_023006459.1 mitogen-activated protein kinase kinase kinase NPK1 isoform X1 [Cucurbita maxima] >XP_023006460.1 mitogen-activated protein kinase kinase kinase NPK1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1208.7 bits (3126), Expect = 0.0e+00
Identity = 607/646 (93.96%), Postives = 625/646 (96.75%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLAPDCDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVVKEDA 60
           MQDFFGSVRRSLVFLAPD DDGG+FGGLVE+IGSSIRKSRNGLFSKHSVRALPPV KEDA
Sbjct: 1   MQDFFGSVRRSLVFLAPDGDDGGKFGGLVERIGSSIRKSRNGLFSKHSVRALPPVAKEDA 60

Query: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           PPIWWRKGELIGCGAFGRVYMGMNLDSGEL+A+KQVSIAANSASREKAQAHIRELEEEVK
Sbjct: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELIAIKQVSIAANSASREKAQAHIRELEEEVK 120

Query: 121 LLKNLSHPNIVRYLGTAREEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGTAREE SLNILLEFVPGGSISSLLGKFGSFPESV++TYTKQLLLG
Sbjct: 121 LLKNLSHPNIVRYLGTAREESSLNILLEFVPGGSISSLLGKFGSFPESVMKTYTKQLLLG 180

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE
Sbjct: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA
Sbjct: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKPMVDSRLNSNDLT 360
           KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQ+PHAAVR SS+ESGK M DSRLNS D+T
Sbjct: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQEPHAAVRVSSLESGKQMADSRLNSKDIT 360

Query: 361 NSTIQRST--GLKDICEMGSLRCSSVFSGNYGSRSNWGSSNFEDDMCQIDDKDLFVSSSI 420
            S IQRST  GLKDICEMGSLRCSSVFSGNY  RSNWGSSNFEDDMCQIDDK+LF SSSI
Sbjct: 361 KSIIQRSTCEGLKDICEMGSLRCSSVFSGNYEVRSNWGSSNFEDDMCQIDDKNLFASSSI 420

Query: 421 KYKSIISSHDLNKSFNPMCEPTDDWACKLDESSELGGNLMELSGIQTGQESTSKGNDFTF 480
           KYKSIISS+DLNKSFNPMCEPTDDWACKLDESSEL GNLMELSGI+TGQESTSKGNDFTF
Sbjct: 421 KYKSIISSNDLNKSFNPMCEPTDDWACKLDESSELRGNLMELSGIRTGQESTSKGNDFTF 480

Query: 481 PCGQSAAEDDEEVTESKIKAFLDEKALELKKLQTPLYEEFRSTINAANALGAVGTENSKS 540
           PCGQS AEDDEEVT+SKI+AFLDEKALELKKLQTPLYEEFRST+NAANALGAVGTENSKS
Sbjct: 481 PCGQSTAEDDEEVTDSKIQAFLDEKALELKKLQTPLYEEFRSTVNAANALGAVGTENSKS 540

Query: 541 VSNLLKLPPKCGSPSKLRGKRLSSVDAANCSSRQSRIKQDSILQDRALQEIQSPQLGEWN 600
           VSN LKLPPKCGSPSKLRGKRLSS+DA NCSSRQSRIKQ SILQDRALQEIQSPQ+GEWN
Sbjct: 541 VSNFLKLPPKCGSPSKLRGKRLSSIDAGNCSSRQSRIKQASILQDRALQEIQSPQIGEWN 600

Query: 601 KLLHTQPESCTPS-FSERQRKWKEELEEELKRKREMMRRGIVGAIH 644
           KLLHTQPESCTP+ FSERQRKWKEELEEELKRKREMMRRG VGAIH
Sbjct: 601 KLLHTQPESCTPTFFSERQRKWKEELEEELKRKREMMRRGTVGAIH 646

BLAST of Lcy01g002090 vs. TAIR 10
Match: AT3G06030.1 (NPK1-related protein kinase 3 )

HSP 1 Score: 697.6 bits (1799), Expect = 9.5e-201
Identity = 395/654 (60.40%), Postives = 461/654 (70.49%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVF----LAPDCDDGGRFGGLVEKIGSSIRKSRNGLFSKHSVRALPPVV 60
           MQD  GSVRRSLVF       D   GG   G V KI SSIR SR GLFSK     LP   
Sbjct: 1   MQDILGSVRRSLVFRSSLAGDDGTSGGGLSGFVGKINSSIRSSRIGLFSK-PPPGLPAPR 60

Query: 61  KEDAPPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELE 120
           KE+AP I WRKGELIGCGAFGRVYMGMNLDSGELLA+KQV IA +SAS+EK Q HIRELE
Sbjct: 61  KEEAPSIRWRKGELIGCGAFGRVYMGMNLDSGELLAIKQVLIAPSSASKEKTQGHIRELE 120

Query: 121 EEVKLLKNLSHPNIVRYLGTAREEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQ 180
           EEV+LLKNLSHPNIVRYLGT RE DSLNIL+EFVPGGSISSLL KFGSFPE VI  YTKQ
Sbjct: 121 EEVQLLKNLSHPNIVRYLGTVRESDSLNILMEFVPGGSISSLLEKFGSFPEPVIIMYTKQ 180

Query: 181 LLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYW 240
           LLLGLEYLH NGIMHRDIKGANILVDNKGCI+LADFGASKKVVELAT+NGAKSMKGTPYW
Sbjct: 181 LLLGLEYLHNNGIMHRDIKGANILVDNKGCIRLADFGASKKVVELATVNGAKSMKGTPYW 240

Query: 241 MAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHL 300
           MAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWS+QYQ+ AA+ HIG TK+HPPIPE L
Sbjct: 241 MAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIGRTKAHPPIPEDL 300

Query: 301 SAEAKDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKPMVDSRLNS 360
           S EAKDFL+KCL +EP+ R +A++LLQHPFV+ + Q+P+ A R S  E G P+    +N 
Sbjct: 301 SPEAKDFLMKCLHKEPSLRLSATELLQHPFVTGKRQEPYPAYRNSLTECGNPITTQGMNV 360

Query: 361 NDLTNSTIQRST--GLKDICEMGSLRCSSVF---SGNYGSRSNWGSSNFEDDMCQIDDKD 420
               NS I+RST  GLKD+CE+GSLR S ++   S N G     G S   DD+CQ D  D
Sbjct: 361 RSSINSLIRRSTCSGLKDVCELGSLRSSIIYPQKSNNSGFGWRDGDS---DDLCQTDMDD 420

Query: 421 LFVSSSIKYKSIISSHDLNKSFNPMCEPTDDWACKLDESSELGGNLMELSGIQTGQEST- 480
           L    S++   +  S DLNKSFNPMC+ TD+W+CK DES ++  +   L   Q  Q  T 
Sbjct: 421 LCNIESVRNNVLSQSTDLNKSFNPMCDSTDNWSCKFDESPKVMKSKSNLLSYQASQLQTG 480

Query: 481 ---SKGNDFTFPCGQSAAEDDEEVTESKIKAFLDEKALELKKLQTPLYEEFRSTINAANA 540
               +    TF  G S AEDD + TE KIK+FLDEKA +LK+LQTPL EEF + +N    
Sbjct: 481 VPCDEETSLTFAGGSSVAEDDYKGTELKIKSFLDEKAQDLKRLQTPLLEEFHNAMNPGIP 540

Query: 541 LGAVGTENSKSVSNLLKLPPKCGSPSKLRGKRLSSVDAANCSSRQSRIKQDSILQDRALQ 600
            GA+G  N   + NL  LP    +P +L  +RLS++  A  S  +S   + ++   R +Q
Sbjct: 541 QGALGDTN---IYNLPNLPSISKTPKRLPSRRLSAISDAMPSPLKS--SKRTLNTSRVMQ 600

Query: 601 EIQSP-QLGEWNKLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRRGIVG 641
               P Q+ E  K    +  + +  FSE +RKW+EEL EEL+R RE +R    G
Sbjct: 601 SGTEPTQVNESTK----KGVNNSRCFSEIRRKWEEELYEELERHRENLRHAGAG 641

BLAST of Lcy01g002090 vs. TAIR 10
Match: AT1G09000.1 (NPK1-related protein kinase 1 )

HSP 1 Score: 613.2 bits (1580), Expect = 2.3e-175
Identity = 373/671 (55.59%), Postives = 448/671 (66.77%), Query Frame = 0

Query: 1   MQDFFGSVRRSLVFLAPDCDDGGRF------GGLVEKIGSSIRKSRNGLFSKHSVRALPP 60
           MQDFFGSVRRSLVF  P  DD  +       G L +KI S IRKS+  +F K S    PP
Sbjct: 1   MQDFFGSVRRSLVF-RPSSDDDNQENQPPFPGVLADKITSCIRKSK--IFIKPSFSPPPP 60

Query: 61  VVKED-APPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIR 120
               D APPI WRKG+LIG GAFG VYMGMNLDSGELLAVKQV IAAN AS+EK QAHI+
Sbjct: 61  ANTVDMAPPISWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQ 120

Query: 121 ELEEEVKLLKNLSHPNIVRYLGTAREEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTY 180
           ELEEEVKLLKNLSHPNIVRYLGT RE+D+LNILLEFVPGGSISSLL KFG FPESV+RTY
Sbjct: 121 ELEEEVKLLKNLSHPNIVRYLGTVREDDTLNILLEFVPGGSISSLLEKFGPFPESVVRTY 180

Query: 181 TKQLLLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGT 240
           T+QLLLGLEYLH + IMHRDIKGANILVDNKGCIKLADFGASK+V ELAT+ GAKSMKGT
Sbjct: 181 TRQLLLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMTGAKSMKGT 240

Query: 241 PYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIP 300
           PYWMAPEVILQTGHSFSADIWSVGCTVIEM TGK PWSQQY+EVAA+F IG TKSHPPIP
Sbjct: 241 PYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIP 300

Query: 301 EHLSAEAKDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKPMVDSR 360
           + LS++AKDFLLKCLQE PN RPTAS+LL+HPFV  ++++  +    S + +    +  +
Sbjct: 301 DTLSSDAKDFLLKCLQEVPNLRPTASELLKHPFVMGKHKESASTDLGSVLNNLSTPLPLQ 360

Query: 361 LNSNDLTNSTIQRSTGLKDICEMGSLRCS------SVFSGNYGSRSNWGSSNFEDDMCQI 420
           +N+   T  +     G  D+C  GSL  S      S+ + N   +++ G    EDDMC I
Sbjct: 361 INNTKSTPDSTCDDVG--DMCNFGSLNYSLVDPVKSIQNKNLWQQNDNGGD--EDDMCLI 420

Query: 421 DDKDLFVSSSIKYKSIISSHDLNKSFNPMCEPTDDWACKLDESSELGGNLMELSGIQTGQ 480
           DD++          ++     L KS + + + +     K DES   G             
Sbjct: 421 DDENFLTFDGEMSSTLEKDCHLKKSCDDISDMSIALKSKFDESPGNG------------- 480

Query: 481 ESTSKGNDFTFPCGQ-SAAEDDEEVTESKIKAFLDEKALELKKLQTPLYEEF-RSTINAA 540
               K +  +  C Q S +EDD+E+TESKIKAFLDEKA +LKKLQTPLYEEF  S I  +
Sbjct: 481 ---EKESTMSMECDQPSYSEDDDELTESKIKAFLDEKAADLKKLQTPLYEEFYNSLITFS 540

Query: 541 NALGAVGTENSK---SVSNLLKLPPKCGSPSK--LRGKRLSSVDAANCS-------SRQS 600
            +       NSK   +    LKLPPK  SPS+  L G    + DA +CS       SR+ 
Sbjct: 541 PSCMESNLSNSKREDTARGFLKLPPKSRSPSRGPLGGSPSRATDATSCSKSPGSGGSREL 600

Query: 601 RIKQDSILQDRALQEIQSPQLGEWNKLL--HTQPESCTPSFSERQRKWKEELEEELKRKR 642
            I       D A Q+  S ++ +W  L+    Q  S   + SE ++KWKEEL++EL+RKR
Sbjct: 601 NINNGG---DEASQDGVSARVTDWRGLVVDTKQELSQCVALSEIEKKWKEELDQELERKR 645

BLAST of Lcy01g002090 vs. TAIR 10
Match: AT1G54960.1 (NPK1-related protein kinase 2 )

HSP 1 Score: 573.2 bits (1476), Expect = 2.7e-163
Identity = 327/588 (55.61%), Postives = 408/588 (69.39%), Query Frame = 0

Query: 61  PPIWWRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVK 120
           PPI WRKG+LIG GAFG VYMGMNLDSGELLAVKQV I +N AS+EK QAHI+ELEEEVK
Sbjct: 19  PPIRWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLITSNCASKEKTQAHIQELEEEVK 78

Query: 121 LLKNLSHPNIVRYLGTAREEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLG 180
           LLKNLSHPNIVRYLGT RE+++LNILLEFVPGGSISSLL KFG+FPESV+RTYT QLLLG
Sbjct: 79  LLKNLSHPNIVRYLGTVREDETLNILLEFVPGGSISSLLEKFGAFPESVVRTYTNQLLLG 138

Query: 181 LEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPE 240
           LEYLH + IMHRDIKGANILVDN+GCIKLADFGASK+V ELATI+GAKSMKGTPYWMAPE
Sbjct: 139 LEYLHNHAIMHRDIKGANILVDNQGCIKLADFGASKQVAELATISGAKSMKGTPYWMAPE 198

Query: 241 VILQTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEA 300
           VILQTGHSFSADIWSVGCTVIEM TGK PWSQQY+E+AA+FHIG TKSHPPIP+++S++A
Sbjct: 199 VILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTKSHPPIPDNISSDA 258

Query: 301 KDFLLKCLQEEPNSRPTASDLLQHPFVSCEYQDPHAAVRASSVESGKPMVDSRLNSNDLT 360
            DFLLKCLQ+EPN RPTAS+LL+HPFV+ + ++  +    S +++    + S L   ++T
Sbjct: 259 NDFLLKCLQQEPNLRPTASELLKHPFVTGKQKESASKDLTSFMDNSCSPLPSEL--TNIT 318

Query: 361 NSTIQRSTGLKDICEMGSLRCS------SVFSGNYGSRSNWGSSNFED-DMCQIDDKDLF 420
           +     S  + DIC +GSL C+      S+ + +   +SN G  + +D DMC IDD++  
Sbjct: 319 SYQTSTSDDVGDICNLGSLTCTLAFPEKSIQNNSLCLKSNNGYDDDDDNDMCLIDDENFL 378

Query: 421 VSSSIKYKSIISSHDLNKSFNPMCEPTDDWACKLDESSELGGNLMELSGIQTGQESTSKG 480
             +     S+ ++ D  KS + M E +D   CK DE+S   GN         G+  T   
Sbjct: 379 TYNGETGPSLDNNTDAKKSCDTMSEISDILKCKFDENS---GN---------GETETKVS 438

Query: 481 NDFTFPCGQSAAEDDEEVTESKIKAFLDEKALELKKLQTPLYEEFRSTINAANALGAVGT 540
            +   P   S +ED+ E+TESKIKAFLD+KA ELKKLQTPLYEEF + +   + +     
Sbjct: 439 MEVDHP---SYSEDENELTESKIKAFLDDKAAELKKLQTPLYEEFYNGMITCSPICMESN 498

Query: 541 ENS----KSVSNLLKLPPKCGSPSKLR-GKRLSSVDAANCSSRQSRIKQDSILQDRALQE 600
            N+    ++    LKLPPK  SPS+   G+  S    A C S+       S         
Sbjct: 499 INNNKREEAPRGFLKLPPKSRSPSQGHIGRSPSRATDAACCSKSPESGNSS--------- 558

Query: 601 IQSPQLGEWNKLLHTQPESCTPSFSERQRKWKEELEEELKRKREMMRR 637
             +P+    +     +  S + + SE +RKWKEEL++EL+RKR  + R
Sbjct: 559 -GAPKNSNASAGAEQESNSQSVALSEIERKWKEELDQELERKRREITR 579

BLAST of Lcy01g002090 vs. TAIR 10
Match: AT1G53570.1 (mitogen-activated protein kinase kinase kinase 3 )

HSP 1 Score: 291.6 bits (745), Expect = 1.6e-78
Identity = 137/264 (51.89%), Postives = 192/264 (72.73%), Query Frame = 0

Query: 65  WRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVKLLKN 124
           W+KG+ +G G FG+VY+G N + G++ A+K+V + ++  + ++    +++L +E+ LL  
Sbjct: 214 WKKGKFLGSGTFGQVYLGFNSEKGKMCAIKEVKVISDDQTSKEC---LKQLNQEINLLNQ 273

Query: 125 LSHPNIVRYLGTAREEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLGLEYL 184
           L HPNIV+Y G+   E++L++ LE+V GGSI  LL  +GSF E VI+ YT+Q+L GL YL
Sbjct: 274 LCHPNIVQYYGSELSEETLSVYLEYVSGGSIHKLLKDYGSFTEPVIQNYTRQILAGLAYL 333

Query: 185 HKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPEVIL- 244
           H    +HRDIKGANILVD  G IKLADFG +K V   +T+    S KG+PYWMAPEV++ 
Sbjct: 334 HGRNTVHRDIKGANILVDPNGEIKLADFGMAKHVTAFSTM---LSFKGSPYWMAPEVVMS 393

Query: 245 QTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEAKDF 304
           Q G++ + DIWS+GCT++EMAT KPPWS Q++ VAA+F IGN+K  P IP+HLS +AK+F
Sbjct: 394 QNGYTHAVDIWSLGCTILEMATSKPPWS-QFEGVAAIFKIGNSKDTPEIPDHLSNDAKNF 453

Query: 305 LLKCLQEEPNSRPTASDLLQHPFV 328
           +  CLQ  P  RPTAS LL+HPF+
Sbjct: 454 IRLCLQRNPTVRPTASQLLEHPFL 470

BLAST of Lcy01g002090 vs. TAIR 10
Match: AT1G53570.2 (mitogen-activated protein kinase kinase kinase 3 )

HSP 1 Score: 291.6 bits (745), Expect = 1.6e-78
Identity = 137/264 (51.89%), Postives = 192/264 (72.73%), Query Frame = 0

Query: 65  WRKGELIGCGAFGRVYMGMNLDSGELLAVKQVSIAANSASREKAQAHIRELEEEVKLLKN 124
           W+KG+ +G G FG+VY+G N + G++ A+K+V + ++  + ++    +++L +E+ LL  
Sbjct: 214 WKKGKFLGSGTFGQVYLGFNSEKGKMCAIKEVKVISDDQTSKEC---LKQLNQEINLLNQ 273

Query: 125 LSHPNIVRYLGTAREEDSLNILLEFVPGGSISSLLGKFGSFPESVIRTYTKQLLLGLEYL 184
           L HPNIV+Y G+   E++L++ LE+V GGSI  LL  +GSF E VI+ YT+Q+L GL YL
Sbjct: 274 LCHPNIVQYYGSELSEETLSVYLEYVSGGSIHKLLKDYGSFTEPVIQNYTRQILAGLAYL 333

Query: 185 HKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATINGAKSMKGTPYWMAPEVIL- 244
           H    +HRDIKGANILVD  G IKLADFG +K V   +T+    S KG+PYWMAPEV++ 
Sbjct: 334 HGRNTVHRDIKGANILVDPNGEIKLADFGMAKHVTAFSTM---LSFKGSPYWMAPEVVMS 393

Query: 245 QTGHSFSADIWSVGCTVIEMATGKPPWSQQYQEVAALFHIGNTKSHPPIPEHLSAEAKDF 304
           Q G++ + DIWS+GCT++EMAT KPPWS Q++ VAA+F IGN+K  P IP+HLS +AK+F
Sbjct: 394 QNGYTHAVDIWSLGCTILEMATSKPPWS-QFEGVAAIFKIGNSKDTPEIPDHLSNDAKNF 453

Query: 305 LLKCLQEEPNSRPTASDLLQHPFV 328
           +  CLQ  P  RPTAS LL+HPF+
Sbjct: 454 IRLCLQRNPTVRPTASQLLEHPFL 470

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q405414.4e-22763.36Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097... [more]
O220421.3e-19960.40Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702... [more]
O220403.3e-17455.59Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana OX=3... [more]
Q9FZ365.5e-16953.96Mitogen-activated protein kinase kinase kinase 2 OS=Arabidopsis thaliana OX=3702... [more]
Q54R828.6e-8253.58Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=... [more]
Match NameE-valueIdentityDescription
A0A6J1KXU20.0e+0094.11mitogen-activated protein kinase kinase kinase NPK1 isoform X2 OS=Cucurbita maxi... [more]
A0A6J1H6610.0e+0093.95mitogen-activated protein kinase kinase kinase NPK1 isoform X2 OS=Cucurbita mosc... [more]
A0A6J1L4Z40.0e+0093.96mitogen-activated protein kinase kinase kinase NPK1 isoform X1 OS=Cucurbita maxi... [more]
A0A6J1H4340.0e+0093.65mitogen-activated protein kinase kinase kinase NPK1 isoform X1 OS=Cucurbita mosc... [more]
A0A6J1CE750.0e+0094.24mitogen-activated protein kinase kinase kinase NPK1 OS=Momordica charantia OX=36... [more]
Match NameE-valueIdentityDescription
XP_023547660.10.0e+0094.73mitogen-activated protein kinase kinase kinase NPK1 isoform X1 [Cucurbita pepo s... [more]
XP_023006461.10.0e+0094.11mitogen-activated protein kinase kinase kinase NPK1 isoform X2 [Cucurbita maxima... [more]
KAG6575002.10.0e+0094.11Mitogen-activated protein kinase kinase kinase NPK1, partial [Cucurbita argyrosp... [more]
XP_022958799.10.0e+0093.95mitogen-activated protein kinase kinase kinase NPK1 isoform X2 [Cucurbita moscha... [more]
XP_023006458.10.0e+0093.96mitogen-activated protein kinase kinase kinase NPK1 isoform X1 [Cucurbita maxima... [more]
Match NameE-valueIdentityDescription
AT3G06030.19.5e-20160.40NPK1-related protein kinase 3 [more]
AT1G09000.12.3e-17555.59NPK1-related protein kinase 1 [more]
AT1G54960.12.7e-16355.61NPK1-related protein kinase 2 [more]
AT1G53570.11.6e-7851.89mitogen-activated protein kinase kinase kinase 3 [more]
AT1G53570.21.6e-7851.89mitogen-activated protein kinase kinase kinase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 98..125
NoneNo IPR availableCOILSCoilCoilcoord: 613..636
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 65..364
e-value: 1.5E-86
score: 292.1
NoneNo IPR availablePIRSRPIRSR037993-2PIRSR037993-2coord: 29..331
e-value: 3.5E-28
score: 96.3
NoneNo IPR availablePIRSRPIRSR000556-2PIRSR000556-2coord: 68..323
e-value: 3.2E-33
score: 112.9
NoneNo IPR availablePIRSRPIRSR037993-1PIRSR037993-1coord: 29..331
e-value: 3.5E-28
score: 96.3
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 71..323
e-value: 5.6E-17
score: 59.5
NoneNo IPR availablePANTHERPTHR48016:SF23MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE NPK1-LIKE ISOFORM X1coord: 1..613
NoneNo IPR availablePANTHERPTHR48016MAP KINASE KINASE KINASE SSK2-RELATED-RELATEDcoord: 1..613
NoneNo IPR availableCDDcd06606STKc_MAPKKKcoord: 65..327
e-value: 9.4817E-173
score: 490.11
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 65..327
e-value: 3.6E-101
score: 352.1
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 66..327
e-value: 3.5E-73
score: 246.2
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 65..327
score: 52.541039
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 189..201
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 71..94
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 65..381

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy01g002090.1Lcy01g002090.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity