Lcy01g001990 (gene) Sponge gourd (P93075) v1

Overview
NameLcy01g001990
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptionbasic 7S globulin-like
LocationChr01: 2341058 .. 2342701 (+)
RNA-Seq ExpressionLcy01g001990
SyntenyLcy01g001990
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGCCCTTATCCAATTCCCACACACATTCCAATCTCTCTCTCTCTCTCTCTCTCTTTCTCTCTTTCTCTCTCTAACCTTATTCCCAAACCGCGGCGAACCTCCGCCGACTGTACCAAATTTCCATGGCTGTTCCCCAATACCCTTCTCTTCTTCTTCTTCTCTGTTTCTTTACCCTCTTCCATGGCGGAACCTTCTCTCTCGTCATTCCGGTCACCAAAGATCCCGTTACTGATCAGTATTTGGCCGCAGTCCACCATGGCTCTCCGATCAAGCCGACCCATCTCGCCGTCGATCTCGGCGGGCCGTCTCTCTGGATGGCCTGTGGCGGATCTTCCTCCAGCCGCCCGATTCCGAGGCGGTCGATTCAGTGCGTTGCGGCCGCGGGCGGTGGTGGTGGATCTGGGTCTGGAGGCGGCGGCGCGTGTGATGTAGTTGCTGGGAATCCGTTTGGGGCTTTGGTAGGGAAGGCCGTGCTGGTTGAGGATACGGTGGCGGTGAGGTCTTTCGACCGGTCAACGGCGGCGGTGATTGTTGCAGTTCATTCTTGTGCTCCGAGGTTTCTTTTGCAGGGTTTGGCTAAATCTGCCAGGGGGATTTTAGGGCTTGGCCGGAATCAGAACTCGCTTCCGGCTCAGATCGCGACGGAGCTCGGTTTTCACCGGCGGTTTTCTGTTTGTCTCTCGTCGACGAACGGCGTCGTCTTTCCGGACAGTGGAAGTCAAGACTCTGTTTATGGATCTGAGATTTCGAGTTCTTTGACTTACACGCCGATTTTGACTAAGAAAATCGACGGCTCGCAGTCGCCGGAGTATTTCATCAACGTTAAGGCCATCAAAGTCGACGGTAACCGTCTCGATCTGAACAAATCGTTACTCGATTTGGACGGCGGCGAAGGCGTCGGAGGGACAAGACTGAGCACGGTGGTTCCGTACACCGCACTAGAGAGTTCGATCTTTAAATCTCTGACGGCAGCGTTCAGGGCGGCCGCGGCGGCGATGAACATAAAAGAAGTGGCTCCGGTGGCGCCGTTCGAGGCGTGTTTTGCGGCGGAGAACATGGAGATGACGGCGACGGGGCCGAAAGTGCCGGAGATTGAACTGATTTTGCAGAGCGAGATGGTGGGATGGAAAATCGCCGGCAGAAATTCGATGGTGAAGGTGAACGATGAAACTTTCTGTTTAGGGTTTGTGGACGGCGGATTGAAGCCGAAAAACGCCGTCGTTTTGGGAGGTTATCAAATGGAAGATATCGTCCTTGACTTCGACATGGGAACTTCGATGCTCGGATTCAGCTCGTCTTTGTTGCAGAGGAAGAGATCTTGCTCGGAATTCTCGCCGGAAAATTTACTGAAAAAAACAGGATGATGATTTGGGGAAGAAGAAACTGAAATTAGGGTAAAATTAAATTTTGGGGAAATTAGGATTTTGCTGTGTACTATTGAGGATATAAATATAAATATATATTTTTTGTTTAATTTTGGGGCAATGTTTTTTAATTTTCTAGTTCCAGCATAACTTTTTTTGGGTTATGACTTGTGACAAATTAAATTAAAAGATGTGTAATGTAATTGAGATCCAATTTTTGTTAAAGCTTTTGGTTGTTCTATGAATTGAATCTTGAATCAATTATCTCTTAATTTTGTTG

mRNA sequence

GGCCCTTATCCAATTCCCACACACATTCCAATCTCTCTCTCTCTCTCTCTCTCTTTCTCTCTTTCTCTCTCTAACCTTATTCCCAAACCGCGGCGAACCTCCGCCGACTGTACCAAATTTCCATGGCTGTTCCCCAATACCCTTCTCTTCTTCTTCTTCTCTGTTTCTTTACCCTCTTCCATGGCGGAACCTTCTCTCTCGTCATTCCGGTCACCAAAGATCCCGTTACTGATCAGTATTTGGCCGCAGTCCACCATGGCTCTCCGATCAAGCCGACCCATCTCGCCGTCGATCTCGGCGGGCCGTCTCTCTGGATGGCCTGTGGCGGATCTTCCTCCAGCCGCCCGATTCCGAGGCGGTCGATTCAGTGCGTTGCGGCCGCGGGCGGTGGTGGTGGATCTGGGTCTGGAGGCGGCGGCGCGTGTGATGTAGTTGCTGGGAATCCGTTTGGGGCTTTGGTAGGGAAGGCCGTGCTGGTTGAGGATACGGTGGCGGTGAGGTCTTTCGACCGGTCAACGGCGGCGGTGATTGTTGCAGTTCATTCTTGTGCTCCGAGGTTTCTTTTGCAGGGTTTGGCTAAATCTGCCAGGGGGATTTTAGGGCTTGGCCGGAATCAGAACTCGCTTCCGGCTCAGATCGCGACGGAGCTCGGTTTTCACCGGCGGTTTTCTGTTTGTCTCTCGTCGACGAACGGCGTCGTCTTTCCGGACAGTGGAAGTCAAGACTCTGTTTATGGATCTGAGATTTCGAGTTCTTTGACTTACACGCCGATTTTGACTAAGAAAATCGACGGCTCGCAGTCGCCGGAGTATTTCATCAACGTTAAGGCCATCAAAGTCGACGGTAACCGTCTCGATCTGAACAAATCGTTACTCGATTTGGACGGCGGCGAAGGCGTCGGAGGGACAAGACTGAGCACGGTGGTTCCGTACACCGCACTAGAGAGTTCGATCTTTAAATCTCTGACGGCAGCGTTCAGGGCGGCCGCGGCGGCGATGAACATAAAAGAAGTGGCTCCGGTGGCGCCGTTCGAGGCGTGTTTTGCGGCGGAGAACATGGAGATGACGGCGACGGGGCCGAAAGTGCCGGAGATTGAACTGATTTTGCAGAGCGAGATGGTGGGATGGAAAATCGCCGGCAGAAATTCGATGGTGAAGGTGAACGATGAAACTTTCTGTTTAGGGTTTGTGGACGGCGGATTGAAGCCGAAAAACGCCGTCGTTTTGGGAGGTTATCAAATGGAAGATATCGTCCTTGACTTCGACATGGGAACTTCGATGCTCGGATTCAGCTCGTCTTTGTTGCAGAGGAAGAGATCTTGCTCGGAATTCTCGCCGGAAAATTTACTGAAAAAAACAGGATGATGATTTGGGGAAGAAGAAACTGAAATTAGGGTAAAATTAAATTTTGGGGAAATTAGGATTTTGCTGTGTACTATTGAGGATATAAATATAAATATATATTTTTTGTTTAATTTTGGGGCAATGTTTTTTAATTTTCTAGTTCCAGCATAACTTTTTTTGGGTTATGACTTGTGACAAATTAAATTAAAAGATGTGTAATGTAATTGAGATCCAATTTTTGTTAAAGCTTTTGGTTGTTCTATGAATTGAATCTTGAATCAATTATCTCTTAATTTTGTTG

Coding sequence (CDS)

ATGGCTGTTCCCCAATACCCTTCTCTTCTTCTTCTTCTCTGTTTCTTTACCCTCTTCCATGGCGGAACCTTCTCTCTCGTCATTCCGGTCACCAAAGATCCCGTTACTGATCAGTATTTGGCCGCAGTCCACCATGGCTCTCCGATCAAGCCGACCCATCTCGCCGTCGATCTCGGCGGGCCGTCTCTCTGGATGGCCTGTGGCGGATCTTCCTCCAGCCGCCCGATTCCGAGGCGGTCGATTCAGTGCGTTGCGGCCGCGGGCGGTGGTGGTGGATCTGGGTCTGGAGGCGGCGGCGCGTGTGATGTAGTTGCTGGGAATCCGTTTGGGGCTTTGGTAGGGAAGGCCGTGCTGGTTGAGGATACGGTGGCGGTGAGGTCTTTCGACCGGTCAACGGCGGCGGTGATTGTTGCAGTTCATTCTTGTGCTCCGAGGTTTCTTTTGCAGGGTTTGGCTAAATCTGCCAGGGGGATTTTAGGGCTTGGCCGGAATCAGAACTCGCTTCCGGCTCAGATCGCGACGGAGCTCGGTTTTCACCGGCGGTTTTCTGTTTGTCTCTCGTCGACGAACGGCGTCGTCTTTCCGGACAGTGGAAGTCAAGACTCTGTTTATGGATCTGAGATTTCGAGTTCTTTGACTTACACGCCGATTTTGACTAAGAAAATCGACGGCTCGCAGTCGCCGGAGTATTTCATCAACGTTAAGGCCATCAAAGTCGACGGTAACCGTCTCGATCTGAACAAATCGTTACTCGATTTGGACGGCGGCGAAGGCGTCGGAGGGACAAGACTGAGCACGGTGGTTCCGTACACCGCACTAGAGAGTTCGATCTTTAAATCTCTGACGGCAGCGTTCAGGGCGGCCGCGGCGGCGATGAACATAAAAGAAGTGGCTCCGGTGGCGCCGTTCGAGGCGTGTTTTGCGGCGGAGAACATGGAGATGACGGCGACGGGGCCGAAAGTGCCGGAGATTGAACTGATTTTGCAGAGCGAGATGGTGGGATGGAAAATCGCCGGCAGAAATTCGATGGTGAAGGTGAACGATGAAACTTTCTGTTTAGGGTTTGTGGACGGCGGATTGAAGCCGAAAAACGCCGTCGTTTTGGGAGGTTATCAAATGGAAGATATCGTCCTTGACTTCGACATGGGAACTTCGATGCTCGGATTCAGCTCGTCTTTGTTGCAGAGGAAGAGATCTTGCTCGGAATTCTCGCCGGAAAATTTACTGAAAAAAACAGGATGA

Protein sequence

MAVPQYPSLLLLLCFFTLFHGGTFSLVIPVTKDPVTDQYLAAVHHGSPIKPTHLAVDLGGPSLWMACGGSSSSRPIPRRSIQCVAAAGGGGGSGSGGGGACDVVAGNPFGALVGKAVLVEDTVAVRSFDRSTAAVIVAVHSCAPRFLLQGLAKSARGILGLGRNQNSLPAQIATELGFHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDGSQSPEYFINVKAIKVDGNRLDLNKSLLDLDGGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIKEVAPVAPFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDETFCLGFVDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLKKTG
Homology
BLAST of Lcy01g001990 vs. ExPASy Swiss-Prot
Match: P0DO21 (Probable aspartic proteinase GIP2 OS=Nicotiana benthamiana OX=4100 GN=GIP2 PE=1 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 3.7e-70
Identity = 163/405 (40.25%), Postives = 235/405 (58.02%), Query Frame = 0

Query: 26  LVIPVTKDPVTDQYLAAVHHGSPIKPTHLAVDLGGPSLWMACGG---SSSSRPIPRRSIQ 85
           L++P+TKD +T QYL  +   +P+ P  L +DLGG  LW+ C     SS+ RP   RS Q
Sbjct: 34  LILPITKDALTLQYLTQIQQRTPLVPVSLTLDLGGQFLWVDCDQGYVSSTYRPARCRSAQ 93

Query: 86  CVAAAGGGGGSG--------SGGGGACDVVAGNPFGALVGKAVLVEDTVAVRSFDRSTAA 145
           C + AG G G G              C ++  N          L  DTV V+S +     
Sbjct: 94  C-SLAGAGSGCGQCFSPPKPGCNNNTCGLLPDNTITRTATSGELASDTVQVQSSNGKNPG 153

Query: 146 VIVA----VHSCAPRFLLQGLAKSARGILGLGRNQNSLPAQIATELGFHRRFSVCLSS-- 205
             V+    +  C   FLL+GLA   +G+ GLGR + SLP+Q + E  F R+F+VCLSS  
Sbjct: 154 RHVSDKDFLFVCGSTFLLEGLASGVKGMAGLGRTRISLPSQFSAEFSFPRKFAVCLSSST 213

Query: 206 -TNGVVFPDSGSQDSVYGSEISSS-LTYTPILTKKIDGSQ-------SPEYFINVKAIKV 265
            + GVV    G    +   E +++  +YTP+    +  +        S EYFI VK+IK+
Sbjct: 214 NSKGVVLFGDGPYTFLPNREFANNDFSYTPLFINPVSTASAFSSREPSSEYFIGVKSIKI 273

Query: 266 DGNRLDLNKSLLDLDGGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIKEVAP 325
           +   + +N +LL +D  +GVGGT++STV PYT LE+SI+ ++T  F      +NI  VA 
Sbjct: 274 NEKVVPINTTLLSID-NQGVGGTKISTVNPYTILETSIYNAVTNFF--VKELVNITRVAS 333

Query: 326 VAPFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDETFCLGFVDGG 385
           VAPF ACF + N+  T  GP VP I+L+LQ+E V W+I G NSMV+V++   CLGFVDGG
Sbjct: 334 VAPFRACFDSRNIASTRVGPAVPSIDLVLQNENVFWRIFGANSMVQVSENVLCLGFVDGG 393

Query: 386 LKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFS 405
           + P+ ++V+GGY +ED +L FD+  S LGF+SS+L R+ +C+ F+
Sbjct: 394 VSPRTSIVVGGYTIEDNLLQFDLARSRLGFTSSILFRQTTCANFN 434

BLAST of Lcy01g001990 vs. ExPASy Swiss-Prot
Match: I1JNS6 (Probable aspartic proteinase GIP1 OS=Glycine max OX=3847 GN=GIP1 PE=1 SV=2)

HSP 1 Score: 228.4 bits (581), Expect = 1.5e-58
Identity = 155/427 (36.30%), Postives = 236/427 (55.27%), Query Frame = 0

Query: 4   PQYPSLL-LLLCFFTLFHGGTFS--LVIPVTKDPVTDQYLAAVHHGSPIKPTHLAVDLGG 63
           P  PSL    L    LF   TF   L+ P++KD  T  Y  +V   +P++PT L + LG 
Sbjct: 3   PPLPSLCNFNLAILFLFLTPTFQIPLIAPISKDDTTQLYTLSVFLKTPLQPTKLHLHLGS 62

Query: 64  PSLWMACGG---SSSSRPIPRRSIQCVAAAGGGGGSGSGGGGACDVVAGNPF--GALVGK 123
              W+ C     SSSS  IP  +  C +       + S     C +   NP     L+  
Sbjct: 63  SLSWVLCDSTYTSSSSHHIPCNTPLCNSFP---SNACSNNSSLCALFPENPVTRNTLLDT 122

Query: 124 AVLVEDTVAVRSFDRSTAAVIVA--VHSCAPRFLLQGLAKSARGILGLGRNQNSLPAQIA 183
           A++  D++A+ ++D S++ V+++  + SCA   LLQGLA +A G+  LGR+  SLPAQI+
Sbjct: 123 ALI--DSLALPTYDASSSLVLISDFIFSCATAHLLQGLAANALGLASLGRSNYSLPAQIS 182

Query: 184 TELGFHRRFSVCL---SSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKI------DG 243
           T L   R F++CL   S+  G     S +   ++ S+I   LTYT ++   +      D 
Sbjct: 183 TSLTSPRSFTLCLPASSANTGAAIFASTASSFLFSSKI--DLTYTQLIVNPVADTVVTDN 242

Query: 244 SQ-SPEYFINVKAIKVDGNRLDLNKSLLDLDGGEGVGGTRLSTVVPYTALESSIFKSLTA 303
            Q S EYFIN+ +IK++G  L +N S+L +D   G GGT++ST  PYT LE+SI++    
Sbjct: 243 PQPSDEYFINLTSIKINGKPLYINSSILTVD-QTGFGGTKISTAEPYTVLETSIYRLFVQ 302

Query: 304 AFRAAAAAMNIKEVAPVAPFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSM 363
            F   ++A N+     V PF  C+ A ++  T  GP VP ++L++ SE V W+I G NSM
Sbjct: 303 RFVNESSAFNLTVTEAVEPFGVCYPAGDLTETRVGPAVPTVDLVMHSEDVFWRIFGGNSM 362

Query: 364 VKV---NDETFCLGFVDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSC 408
           V+V     + +CLGFVDGG + +  +V+GG+Q+ED ++ FD+ ++  GF+S+LL +   C
Sbjct: 363 VRVAKGGVDVWCLGFVDGGTRGRTPIVIGGHQLEDNLMQFDLDSNRFGFTSTLLLQDAKC 421

BLAST of Lcy01g001990 vs. ExPASy Swiss-Prot
Match: P82952 (Gamma conglutin 1 OS=Prunus dulcis OX=3755 GN=Cgamma1 PE=1 SV=2)

HSP 1 Score: 197.2 bits (500), Expect = 3.6e-49
Identity = 138/398 (34.67%), Postives = 206/398 (51.76%), Query Frame = 0

Query: 26  LVIPVTKDPVTDQYLAAVHHGSPIKPTHLAVDLGGPSLWMACGG---SSSSRPIPRRSIQ 85
           LV+ V KD  T+ ++  +H  +P+      +DL G  L + C     SS+ +     S Q
Sbjct: 38  LVLKVQKDRATNLHVVQIHKRTPLVQFPFVIDLTGRFLSVNCENQYTSSTYKAPVCHSSQ 97

Query: 86  CVAAAGGGGGSGSGG-------GGACDVVAGNPFGALVGKAVLVEDTVAVRSFDRSTAAV 145
           C  A      + S           AC ++  NP      +  L ED + + S   S+   
Sbjct: 98  CARANSHTCRTCSSSKTRPGCHTNACGLLTTNPVTQQSAQGELAEDVLKIPSTQGSSPGP 157

Query: 146 IVA----VHSCAPRFLLQ-GLAKSARGILGLGRNQNSLPAQIATELGFHRRFSVCLSST- 205
           +V     + +CAP  +LQ GL K+ +G+ GLG +  SLP Q+A+  GF  +F+VCL+S+ 
Sbjct: 158 MVTYPHFLFACAPSNILQKGLPKNVQGVAGLGHSPISLPYQLASHFGFPPKFAVCLTSSP 217

Query: 206 --NGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDGSQSPEYFINVKAIKVDGNRLDLN 265
             NG VF   G      G ++S  LTY P         Q  EY+INV++ K+       N
Sbjct: 218 GKNGAVFFGEGPYFMKPGIDVSRQLTYAPFTI-----GQQGEYYINVQSFKI-------N 277

Query: 266 KSLLDLDGGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIKEVAPVAPFEACF 325
            ++L      G GG  +ST  PYT L++ IF++L   F        +  V PVAPF ACF
Sbjct: 278 NAMLPSIPKGGFGGAMISTTTPYTTLQTPIFRALNQLF--MNQLRGVPHVKPVAPFGACF 337

Query: 326 AAENMEMTATGPKVPEIELILQSEM-VGWKIAGRNSMVKVNDETFCLGFVDGGLKPKNAV 385
            A  +  +  GP VP I+L+L ++  + W+I G N+M++      CL FVDGG++PK  +
Sbjct: 338 DANRIPTSKMGPTVPSIDLVLDNKKNIMWRIFGANAMIQPRPGVMCLAFVDGGMRPKAPI 397

Query: 386 VLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFS 405
           V+G  Q+ED +L FD+  S LGFSSSLL R+ +C+ F+
Sbjct: 398 VIGTQQLEDNLLQFDLMNSRLGFSSSLLFRRTNCANFN 421

BLAST of Lcy01g001990 vs. ExPASy Swiss-Prot
Match: Q42369 (Gamma conglutin 1 OS=Lupinus angustifolius OX=3871 GN=LOC109345795 PE=1 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 7.1e-37
Identity = 127/407 (31.20%), Postives = 193/407 (47.42%), Query Frame = 0

Query: 26  LVIPVTKDPVTDQYLAAVHHGSPIKPTHLAVDLGGPSLWMACGG--SSSSRPIP------ 85
           LV+PV +D  T  + A +H  +P+    L +DL G  LW+ C    SSS+   P      
Sbjct: 47  LVLPVQEDASTGLHWANIHKRTPLMQVPLLLDLNGKHLWVTCSQHYSSSTYQAPFCHSTQ 106

Query: 86  ---RRSIQCVAAAGGGGGSGSGGGGACDVVAGNPFGALVGKAVLVEDTVAVRSFDRSTAA 145
                + QC                 C +++ NP     G   L +D +A+ S   S   
Sbjct: 107 CSRANTHQCFTCTDSTTTRPGCHNNTCGLLSSNPVTQESGLGELAQDVLAIHSTHGSKLG 166

Query: 146 VIVAV----HSCAPRFLLQ-GLAKSARGILGLGRNQNSLPAQIATELGFHRRFSVCL--- 205
            +V V     SCAP FL Q GL  + +G LGLG+   SL  Q+ +  G  R+FSVCL   
Sbjct: 167 PMVKVPQFLFSCAPSFLAQKGLPNNVQGALGLGQAPISLQNQLFSHFGLKRQFSVCLSRY 226

Query: 206 SSTNGVV----FPDSGSQDSVYGS-EISSSLTYTPILTKKIDGSQSPEYFINVKAIKVDG 265
           S++NG +      D  + + ++ S ++   L YTP+       S+  EYFI V AI+V+ 
Sbjct: 227 STSNGAILFGDINDPNNNNYIHNSLDVLHDLVYTPLTI-----SKQGEYFIQVNAIRVNK 286

Query: 266 NRL------DLNKSLLDLDGGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIK 325
           + +       ++ S     G   +GG  ++T  PYT L  SIF+  T  F  A       
Sbjct: 287 HLVIPTKNPFISPSSTSYHGSGEIGGALITTTHPYTVLSHSIFEVFTQVF--ANNMPKQA 346

Query: 326 EVAPVAPFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDETFCLGF 385
           +V  V PF  C+ +  +   A     P ++LIL      W+I+  N MV+  D   CLGF
Sbjct: 347 QVKAVGPFGLCYDSRKISGGA-----PSVDLILDKNDAVWRISSENFMVQAQDGVSCLGF 406

Query: 386 VDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGF-SSSLLQRKRSCS 402
           VDGG+  +  + LG + +E+ ++ FD+  S +GF S+SL    ++CS
Sbjct: 407 VDGGVHARAGIALGAHHLEENLVVFDLERSRVGFNSNSLKSYGKTCS 441

BLAST of Lcy01g001990 vs. ExPASy Swiss-Prot
Match: Q9FSH9 (Gamma conglutin 1 OS=Lupinus albus OX=3870 GN=Cgamma PE=1 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 1.6e-36
Identity = 123/409 (30.07%), Postives = 193/409 (47.19%), Query Frame = 0

Query: 26  LVIPVTKDPVTDQYLAAVHHGSPIKPTHLAVDLGGPSLWMACGG--SSSSRPIP------ 85
           LV+P+ +D  T  +   +   +P+    + +DL G  LW+ C    SSS+   P      
Sbjct: 48  LVLPIQQDASTKLHWGNILKRTPLMQVPVLLDLNGKHLWVTCSQHYSSSTYQAPFCHSTQ 107

Query: 86  ---RRSIQCVAAAGGGGGSGSGGGGACDVVAGNPFGALVGKAVLVEDTVAVRSFDRSTAA 145
                + QC                 C +++ NP     G   L +D +A+ S   S   
Sbjct: 108 CSRANTHQCFTCTDSTTSRPGCHNNTCGLISSNPVTQESGLGELAQDVLALHSTHGSKLG 167

Query: 146 VIVAV----HSCAPRFLLQ-GLAKSARGILGLGRNQNSLPAQIATELGFHRRFSVCLSS- 205
            +V +     SCAP FL Q GL  + +G LGLG    SLP Q+ +  G  R+F++CLSS 
Sbjct: 168 SLVKIPQFLFSCAPTFLTQKGLPNNVQGALGLGHAPISLPNQLFSHFGLKRQFTMCLSSY 227

Query: 206 --TNGVV----FPDSGSQDSVYGS-EISSSLTYTPILTKKIDGSQSPEYFINVKAIKVDG 265
             +NG +      D  + + ++ S ++   + YTP+       S+  EYFI V AI+V+ 
Sbjct: 228 PTSNGAILFGDINDPNNNNYIHNSLDVLHDMVYTPLTI-----SKQGEYFIQVSAIRVNK 287

Query: 266 NRL--DLNKSLLDLDGGEG------VGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMN 325
           + +    N S+              +GG  ++T  PYT L  SIF+  T  F  A     
Sbjct: 288 HMVIPTKNPSMFPSSSSSSYHESSEIGGAMITTTNPYTVLRHSIFEVFTQVF--ANNVPK 347

Query: 326 IKEVAPVAPFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDETFCL 385
             +V  V PF  C+  + +        VP ++LI+    V W+I+G N MV+  D   CL
Sbjct: 348 QAQVKAVGPFGLCYDTKKI-----SGGVPSVDLIMDKSDVVWRISGENLMVQAQDGVSCL 407

Query: 386 GFVDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGF-SSSLLQRKRSCS 402
           GFVDGG+  +  + LG +Q+E+ ++ FD+  S +GF ++SL    +SCS
Sbjct: 408 GFVDGGVHTRAGIALGTHQLEENLVVFDLARSRVGFNTNSLKSHGKSCS 444

BLAST of Lcy01g001990 vs. ExPASy TrEMBL
Match: A0A6J1EQJ3 (basic 7S globulin-like OS=Cucurbita moschata OX=3662 GN=LOC111436893 PE=4 SV=1)

HSP 1 Score: 687.6 bits (1773), Expect = 3.3e-194
Identity = 364/412 (88.35%), Postives = 380/412 (92.23%), Query Frame = 0

Query: 1   MAVPQYPSLLLLLCFFTLFHGGTFSLVIPVTKDPVTDQYLAAVHHGSPIKPTHLAVDLGG 60
           MAVPQ  SLLLL+CFF+ FHG TFSLVIPVTKD VT+QYLA+V+HGSPIKP HL VDLGG
Sbjct: 1   MAVPQSHSLLLLICFFSFFHGRTFSLVIPVTKDSVTNQYLASVYHGSPIKPIHLVVDLGG 60

Query: 61  PSLWMACGGSSSSRPIPRRSIQCVAAAGGGGGSGSGGGGACDVVAGNPFGALVGKAVLVE 120
           PSLWMACG S+SSR I  RSIQCVAA GGG  SGS GGGAC+VVAGNPFGALVGKA+LVE
Sbjct: 61  PSLWMACGSSTSSRTIQSRSIQCVAATGGGSRSGS-GGGACEVVAGNPFGALVGKALLVE 120

Query: 121 DTVAVRSFDRSTAAVIVAVHSCAPRFLLQGLAKSARGILGLGRNQNSLPAQIATELGFHR 180
           DTVAVR+ +RSTAAVIVAVHSCAPRFLLQGLAKSA+GILGLGRNQ SLPAQIATELGFHR
Sbjct: 121 DTVAVRALERSTAAVIVAVHSCAPRFLLQGLAKSAKGILGLGRNQISLPAQIATELGFHR 180

Query: 181 RFSVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDGSQSPEYFINVKAIKVD 240
           RFSVCLSSTNGVVFPDS SQDSVYGSEISSSLTYTPILTK IDGSQS EYFINVKAIKVD
Sbjct: 181 RFSVCLSSTNGVVFPDSRSQDSVYGSEISSSLTYTPILTKIIDGSQSAEYFINVKAIKVD 240

Query: 241 GNRLDLNKSLLDLDGGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIKEVAPV 300
           GNRLDLNKSLLDLDG +G GGTRLSTVVPYTALESSIFKSLTAAFR AA AMN+KEVAPV
Sbjct: 241 GNRLDLNKSLLDLDGEDG-GGTRLSTVVPYTALESSIFKSLTAAFREAARAMNMKEVAPV 300

Query: 301 APFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDETFCLGFVDGGL 360
           A FEACF++ENMEMTA GPKVP IELILQSEMV WKI GRNSMVKVNDETFCL  VDGGL
Sbjct: 301 ASFEACFSSENMEMTAAGPKVPMIELILQSEMVRWKIDGRNSMVKVNDETFCLAIVDGGL 360

Query: 361 KPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLKKT 413
           KPKNAVVLGGYQMEDIVLDFDMG+SMLGFSSSLLQRKRSCSEFSPENLLK T
Sbjct: 361 KPKNAVVLGGYQMEDIVLDFDMGSSMLGFSSSLLQRKRSCSEFSPENLLKST 410

BLAST of Lcy01g001990 vs. ExPASy TrEMBL
Match: A0A0A0KHJ8 (Basic 7S globulin 2 small subunit OS=Cucumis sativus OX=3659 GN=Csa_6G237600 PE=4 SV=1)

HSP 1 Score: 681.0 bits (1756), Expect = 3.1e-192
Identity = 358/416 (86.06%), Postives = 377/416 (90.62%), Query Frame = 0

Query: 1   MAVPQYPSLLLLLCFFTLFHGGTFSLVIPVTKDPVTDQYLAAVHHGSPIKPTHLAVDLGG 60
           MAVP +P LLLLL   TLFHG TFSLVIP+TKD +T+QY+A V HGSPIKP HLAVDLGG
Sbjct: 1   MAVPHFPFLLLLLSSLTLFHGQTFSLVIPLTKDSLTNQYVATVFHGSPIKPVHLAVDLGG 60

Query: 61  PSLWMACGGSSSSRPIPRRSIQCVAAAGGGGGSGSGGGGACDVVAGNPFGALVGKAVLVE 120
            SLWMACGGSSSSR IP RSIQC+AA  GGG SGS  GGACDV+AGNPFG L GKA+LVE
Sbjct: 61  QSLWMACGGSSSSRSIPSRSIQCIAAT-GGGRSGS-VGGACDVIAGNPFGDLEGKAILVE 120

Query: 121 DTVAVRSFDRSTAAVIVAVHSCAPRFLLQGLAKSARGILGLGRNQNSLPAQIATELGFHR 180
           DTVAVRS DRSTAAVIVA+HSCAPRFLLQGLAKS +G+LGLGRNQ SLPAQIATELG HR
Sbjct: 121 DTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSVKGVLGLGRNQISLPAQIATELGSHR 180

Query: 181 RFSVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDGSQSPEYFINVKAIKVD 240
           RFS+CLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKID  QSPEYFINVKAIKVD
Sbjct: 181 RFSLCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDALQSPEYFINVKAIKVD 240

Query: 241 GNRLDLNKSLLDLDG---GEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIKEV 300
           GNRLDLNKSLLDL+G   GEG GGTRLSTVVPYT LESSIF SLTAAFRAAAAAMN+KEV
Sbjct: 241 GNRLDLNKSLLDLEGVGDGEGGGGTRLSTVVPYTVLESSIFNSLTAAFRAAAAAMNMKEV 300

Query: 301 APVAPFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDETFCLGFVD 360
           APVAPFE CF +ENMEMTA GPKVPEIELILQSEMVGWKI GRNSMVKVNDE +CLGFVD
Sbjct: 301 APVAPFEVCFESENMEMTAAGPKVPEIELILQSEMVGWKIYGRNSMVKVNDEAYCLGFVD 360

Query: 361 GGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLKKTG 414
           GGLKP+NA+VLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSP NLL+ TG
Sbjct: 361 GGLKPRNAIVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPVNLLESTG 414

BLAST of Lcy01g001990 vs. ExPASy TrEMBL
Match: A0A6J1H4R8 (basic 7S globulin-like OS=Cucurbita moschata OX=3662 GN=LOC111460386 PE=4 SV=1)

HSP 1 Score: 679.5 bits (1752), Expect = 8.9e-192
Identity = 359/416 (86.30%), Postives = 375/416 (90.14%), Query Frame = 0

Query: 1   MAVPQYPSLL---LLLCFFTLFHGGTFSLVIPVTKDPVTDQYLAAVHHGSPIKPTHLAVD 60
           MAVP+   LL   LLLCF  LFHGG FSLVIPV KD VT+QYLA ++HGSPIKP HLAVD
Sbjct: 1   MAVPESRLLLLLRLLLCFSALFHGGAFSLVIPVNKDSVTNQYLATLYHGSPIKPVHLAVD 60

Query: 61  LGGPSLWMACGGSSSSRPIPRRSIQCVAAAGGGGGSGSGGGGACDVVAGNPFGALVGKAV 120
           LGG  LWMACGGSSSSRPI  RSIQC+AA GGG    S  G ACDV+AGNPFG L GKAV
Sbjct: 61  LGGQFLWMACGGSSSSRPILSRSIQCIAAVGGGRSGSS--GSACDVIAGNPFGDLEGKAV 120

Query: 121 LVEDTVAVRSFDRSTAAVIVAVHSCAPRFLLQGLAKSARGILGLGRNQNSLPAQIATELG 180
           LVEDTVAVRS DRSTAAVIVA+HSCAPRFLLQGLA+SA+G+LGLGRNQ SLPAQIATELG
Sbjct: 121 LVEDTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAESAKGVLGLGRNQISLPAQIATELG 180

Query: 181 FHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDGSQSPEYFINVKAI 240
           FHRRFSVCLSSTNGVVFPDSG+ DSVYGSEISSSLTYTPILTKKID SQSPEYFINVKAI
Sbjct: 181 FHRRFSVCLSSTNGVVFPDSGNLDSVYGSEISSSLTYTPILTKKIDASQSPEYFINVKAI 240

Query: 241 KVDGNRLDLNKSLLDLDGGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIKEV 300
           KVDGNRLDLNKSLLDLDGG G GGTRLSTVVPYT LESSIF SLTAAFRAAAAAMN+KEV
Sbjct: 241 KVDGNRLDLNKSLLDLDGGSG-GGTRLSTVVPYTTLESSIFNSLTAAFRAAAAAMNMKEV 300

Query: 301 APVAPFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDETFCLGFVD 360
           APVAPFEACF +ENMEMTATGPKVPEIELILQSEMVGWKI GRNSMVKVNDET CLGFVD
Sbjct: 301 APVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFVD 360

Query: 361 GGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLKKTG 414
           GGLKP+NAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRK+SCSEFSPENL  + G
Sbjct: 361 GGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPENLPIRAG 413

BLAST of Lcy01g001990 vs. ExPASy TrEMBL
Match: A0A6J1KM09 (basic 7S globulin-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111494504 PE=4 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 1.3e-190
Identity = 359/412 (87.14%), Postives = 377/412 (91.50%), Query Frame = 0

Query: 1   MAVPQYPSLLLLLCFFTLFHGGTFSLVIPVTKDPVTDQYLAAVHHGSPIKPTHLAVDLGG 60
           MAVPQ  SLLLL+CFF+ FHG TFSLVIPVTKD VT+QYLA+V+HGSPIKP HLAVDLGG
Sbjct: 1   MAVPQSHSLLLLICFFSFFHGRTFSLVIPVTKDSVTNQYLASVYHGSPIKPIHLAVDLGG 60

Query: 61  PSLWMACGGSSSSRPIPRRSIQCVAAAGGGGGSGSGGGGACDVVAGNPFGALVGKAVLVE 120
           PSLWMACG S+SSR I  RSIQCVAA G G  SGS GGGAC+VVA NPFGALVGKA+LVE
Sbjct: 61  PSLWMACGSSTSSRTIQSRSIQCVAATGDGSRSGS-GGGACEVVARNPFGALVGKALLVE 120

Query: 121 DTVAVRSFDRSTAAVIVAVHSCAPRFLLQGLAKSARGILGLGRNQNSLPAQIATELGFHR 180
           DTVAVR+  RSTAAVIVAVHSCAPRFLLQGLAKSA+GILGLGRNQ SLPAQIATELGFHR
Sbjct: 121 DTVAVRALARSTAAVIVAVHSCAPRFLLQGLAKSAKGILGLGRNQISLPAQIATELGFHR 180

Query: 181 RFSVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDGSQSPEYFINVKAIKVD 240
           RFSVCLSSTNGVVFP+SGSQDSVYGSEIS+SLTYTPILTK IDGSQS EYFINVKAIKVD
Sbjct: 181 RFSVCLSSTNGVVFPESGSQDSVYGSEISNSLTYTPILTKIIDGSQSAEYFINVKAIKVD 240

Query: 241 GNRLDLNKSLLDLDGGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIKEVAPV 300
           GNRL L+KSLLDLD  +G GGTRLSTVVPYTALESSIFKSLTAAFR AA AMN+KEVAPV
Sbjct: 241 GNRLVLDKSLLDLDSEDG-GGTRLSTVVPYTALESSIFKSLTAAFREAARAMNMKEVAPV 300

Query: 301 APFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDETFCLGFVDGGL 360
           A FE CFA+ENMEMTATGPKVP+IELILQSEMV WK  GRNSMVKVNDETFCL  VDGGL
Sbjct: 301 ASFEVCFASENMEMTATGPKVPKIELILQSEMVRWKFDGRNSMVKVNDETFCLAIVDGGL 360

Query: 361 KPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLKKT 413
           KPKNAVVLGGYQMEDIVLDFDMG+SMLGFSSSLLQRKRSCSEFSPENLLK T
Sbjct: 361 KPKNAVVLGGYQMEDIVLDFDMGSSMLGFSSSLLQRKRSCSEFSPENLLKST 410

BLAST of Lcy01g001990 vs. ExPASy TrEMBL
Match: A0A6J1KHR1 (basic 7S globulin-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111494504 PE=4 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 1.3e-190
Identity = 359/412 (87.14%), Postives = 377/412 (91.50%), Query Frame = 0

Query: 1   MAVPQYPSLLLLLCFFTLFHGGTFSLVIPVTKDPVTDQYLAAVHHGSPIKPTHLAVDLGG 60
           MAVPQ  SLLLL+CFF+ FHG TFSLVIPVTKD VT+QYLA+V+HGSPIKP HLAVDLGG
Sbjct: 1   MAVPQSHSLLLLICFFSFFHGRTFSLVIPVTKDSVTNQYLASVYHGSPIKPIHLAVDLGG 60

Query: 61  PSLWMACGGSSSSRPIPRRSIQCVAAAGGGGGSGSGGGGACDVVAGNPFGALVGKAVLVE 120
           PSLWMACG S+SSR I  RSIQCVAA G G  SGS GGGAC+VVA NPFGALVGKA+LVE
Sbjct: 61  PSLWMACGSSTSSRTIQSRSIQCVAATGDGSRSGS-GGGACEVVARNPFGALVGKALLVE 120

Query: 121 DTVAVRSFDRSTAAVIVAVHSCAPRFLLQGLAKSARGILGLGRNQNSLPAQIATELGFHR 180
           DTVAVR+  RSTAAVIVAVHSCAPRFLLQGLAKSA+GILGLGRNQ SLPAQIATELGFHR
Sbjct: 121 DTVAVRALARSTAAVIVAVHSCAPRFLLQGLAKSAKGILGLGRNQISLPAQIATELGFHR 180

Query: 181 RFSVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDGSQSPEYFINVKAIKVD 240
           RFSVCLSSTNGVVFP+SGSQDSVYGSEIS+SLTYTPILTK IDGSQS EYFINVKAIKVD
Sbjct: 181 RFSVCLSSTNGVVFPESGSQDSVYGSEISNSLTYTPILTKIIDGSQSAEYFINVKAIKVD 240

Query: 241 GNRLDLNKSLLDLDGGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIKEVAPV 300
           GNRL L+KSLLDLD  +G GGTRLSTVVPYTALESSIFKSLTAAFR AA AMN+KEVAPV
Sbjct: 241 GNRLVLDKSLLDLDSEDG-GGTRLSTVVPYTALESSIFKSLTAAFREAARAMNMKEVAPV 300

Query: 301 APFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDETFCLGFVDGGL 360
           A FE CFA+ENMEMTATGPKVP+IELILQSEMV WK  GRNSMVKVNDETFCL  VDGGL
Sbjct: 301 ASFEVCFASENMEMTATGPKVPKIELILQSEMVRWKFDGRNSMVKVNDETFCLAIVDGGL 360

Query: 361 KPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLKKT 413
           KPKNAVVLGGYQMEDIVLDFDMG+SMLGFSSSLLQRKRSCSEFSPENLLK T
Sbjct: 361 KPKNAVVLGGYQMEDIVLDFDMGSSMLGFSSSLLQRKRSCSEFSPENLLKST 410

BLAST of Lcy01g001990 vs. NCBI nr
Match: XP_022930452.1 (basic 7S globulin-like [Cucurbita moschata])

HSP 1 Score: 687.6 bits (1773), Expect = 6.7e-194
Identity = 364/412 (88.35%), Postives = 380/412 (92.23%), Query Frame = 0

Query: 1   MAVPQYPSLLLLLCFFTLFHGGTFSLVIPVTKDPVTDQYLAAVHHGSPIKPTHLAVDLGG 60
           MAVPQ  SLLLL+CFF+ FHG TFSLVIPVTKD VT+QYLA+V+HGSPIKP HL VDLGG
Sbjct: 1   MAVPQSHSLLLLICFFSFFHGRTFSLVIPVTKDSVTNQYLASVYHGSPIKPIHLVVDLGG 60

Query: 61  PSLWMACGGSSSSRPIPRRSIQCVAAAGGGGGSGSGGGGACDVVAGNPFGALVGKAVLVE 120
           PSLWMACG S+SSR I  RSIQCVAA GGG  SGS GGGAC+VVAGNPFGALVGKA+LVE
Sbjct: 61  PSLWMACGSSTSSRTIQSRSIQCVAATGGGSRSGS-GGGACEVVAGNPFGALVGKALLVE 120

Query: 121 DTVAVRSFDRSTAAVIVAVHSCAPRFLLQGLAKSARGILGLGRNQNSLPAQIATELGFHR 180
           DTVAVR+ +RSTAAVIVAVHSCAPRFLLQGLAKSA+GILGLGRNQ SLPAQIATELGFHR
Sbjct: 121 DTVAVRALERSTAAVIVAVHSCAPRFLLQGLAKSAKGILGLGRNQISLPAQIATELGFHR 180

Query: 181 RFSVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDGSQSPEYFINVKAIKVD 240
           RFSVCLSSTNGVVFPDS SQDSVYGSEISSSLTYTPILTK IDGSQS EYFINVKAIKVD
Sbjct: 181 RFSVCLSSTNGVVFPDSRSQDSVYGSEISSSLTYTPILTKIIDGSQSAEYFINVKAIKVD 240

Query: 241 GNRLDLNKSLLDLDGGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIKEVAPV 300
           GNRLDLNKSLLDLDG +G GGTRLSTVVPYTALESSIFKSLTAAFR AA AMN+KEVAPV
Sbjct: 241 GNRLDLNKSLLDLDGEDG-GGTRLSTVVPYTALESSIFKSLTAAFREAARAMNMKEVAPV 300

Query: 301 APFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDETFCLGFVDGGL 360
           A FEACF++ENMEMTA GPKVP IELILQSEMV WKI GRNSMVKVNDETFCL  VDGGL
Sbjct: 301 ASFEACFSSENMEMTAAGPKVPMIELILQSEMVRWKIDGRNSMVKVNDETFCLAIVDGGL 360

Query: 361 KPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLKKT 413
           KPKNAVVLGGYQMEDIVLDFDMG+SMLGFSSSLLQRKRSCSEFSPENLLK T
Sbjct: 361 KPKNAVVLGGYQMEDIVLDFDMGSSMLGFSSSLLQRKRSCSEFSPENLLKST 410

BLAST of Lcy01g001990 vs. NCBI nr
Match: KAG7026255.1 (Basic 7S globulin, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 686.8 bits (1771), Expect = 1.2e-193
Identity = 366/413 (88.62%), Postives = 382/413 (92.49%), Query Frame = 0

Query: 1   MAVPQYPSLLLLL-CFFTLFHGGTFSLVIPVTKDPVTDQYLAAVHHGSPIKPTHLAVDLG 60
           MAVPQ  SLLLLL CFF+ FHG TFSLVIPVTKD VT+QYLA+V+HGSPIKP HLAVDLG
Sbjct: 1   MAVPQSHSLLLLLICFFSFFHGRTFSLVIPVTKDSVTNQYLASVYHGSPIKPIHLAVDLG 60

Query: 61  GPSLWMACGGSSSSRPIPRRSIQCVAAAGGGGGSGSGGGGACDVVAGNPFGALVGKAVLV 120
           GPSLWMACG S+SSR I  RSIQCVAA GGG  SGS GGGAC+VVAGNPFGALVGKA+LV
Sbjct: 61  GPSLWMACGSSTSSRTIQSRSIQCVAATGGGSRSGS-GGGACEVVAGNPFGALVGKALLV 120

Query: 121 EDTVAVRSFDRSTAAVIVAVHSCAPRFLLQGLAKSARGILGLGRNQNSLPAQIATELGFH 180
           EDTVAVR+ +RSTAAVIVAVHSCAPRFLLQGLAKSA+GILGLGRNQ SLPAQIATELGFH
Sbjct: 121 EDTVAVRALERSTAAVIVAVHSCAPRFLLQGLAKSAKGILGLGRNQISLPAQIATELGFH 180

Query: 181 RRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDGSQSPEYFINVKAIKV 240
           RRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTK IDGSQS EYFINVKAIKV
Sbjct: 181 RRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKIIDGSQSAEYFINVKAIKV 240

Query: 241 DGNRLDLNKSLLDLDGGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIKEVAP 300
           DGNRLDLNKSLLDLDG +G GGTRLSTVVPYTALESSIFKSLTAAFR AA AMN+KEVAP
Sbjct: 241 DGNRLDLNKSLLDLDGEDG-GGTRLSTVVPYTALESSIFKSLTAAFREAARAMNMKEVAP 300

Query: 301 VAPFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDETFCLGFVDGG 360
           VA FEACF++ENME+TA GPKVP IELILQSEMV WKI GRNSMVKVNDETFCL  VDGG
Sbjct: 301 VASFEACFSSENMEITAAGPKVPMIELILQSEMVRWKIDGRNSMVKVNDETFCLAIVDGG 360

Query: 361 LKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLKKT 413
           LKPKNAVVLGGYQMEDIVLDFDMG+SMLGFSSSLLQRKRSCSEFSPENLLK T
Sbjct: 361 LKPKNAVVLGGYQMEDIVLDFDMGSSMLGFSSSLLQRKRSCSEFSPENLLKST 411

BLAST of Lcy01g001990 vs. NCBI nr
Match: XP_023515016.1 (basic 7S globulin-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 683.7 bits (1763), Expect = 9.7e-193
Identity = 363/412 (88.11%), Postives = 379/412 (91.99%), Query Frame = 0

Query: 1   MAVPQYPSLLLLLCFFTLFHGGTFSLVIPVTKDPVTDQYLAAVHHGSPIKPTHLAVDLGG 60
           MAVPQ  SLLLL+CFF+ FHG TFSLVIPVTKD VT+QYLA+V+HGSPIKP  LAVDLGG
Sbjct: 1   MAVPQSHSLLLLICFFSFFHGRTFSLVIPVTKDSVTNQYLASVYHGSPIKPIRLAVDLGG 60

Query: 61  PSLWMACGGSSSSRPIPRRSIQCVAAAGGGGGSGSGGGGACDVVAGNPFGALVGKAVLVE 120
           PSLWMACG S+SSR I  RSIQCVAA GGG  SGS GGGAC+VVAGN FGALVGKA+LVE
Sbjct: 61  PSLWMACGSSTSSRTIQSRSIQCVAATGGGSRSGS-GGGACEVVAGNSFGALVGKALLVE 120

Query: 121 DTVAVRSFDRSTAAVIVAVHSCAPRFLLQGLAKSARGILGLGRNQNSLPAQIATELGFHR 180
           DTVAVR+ +RSTAAVIVAVHSCAPRFLLQGLAKSA+GILGLGRNQ SLPAQIATELGFHR
Sbjct: 121 DTVAVRALERSTAAVIVAVHSCAPRFLLQGLAKSAKGILGLGRNQISLPAQIATELGFHR 180

Query: 181 RFSVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDGSQSPEYFINVKAIKVD 240
           RFSVCLSSTNGVVFPDSGSQDSVYGSEIS+SLTYTPILTK IDGSQS EYFINVKAIKVD
Sbjct: 181 RFSVCLSSTNGVVFPDSGSQDSVYGSEISNSLTYTPILTKIIDGSQSAEYFINVKAIKVD 240

Query: 241 GNRLDLNKSLLDLDGGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIKEVAPV 300
           GNRLDLNKS LDLDG +G GGTRLSTVVPYTALESSIFKSLTAAFR AA AMN+KEVAPV
Sbjct: 241 GNRLDLNKSQLDLDGEDG-GGTRLSTVVPYTALESSIFKSLTAAFREAARAMNMKEVAPV 300

Query: 301 APFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDETFCLGFVDGGL 360
           A FEACFA+ENMEMTA GPKVP IELILQSEMV WKI GRNSMVKVNDETFCL  VDGGL
Sbjct: 301 ASFEACFASENMEMTAAGPKVPMIELILQSEMVRWKIDGRNSMVKVNDETFCLAIVDGGL 360

Query: 361 KPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLKKT 413
           KPKNAVVLGGYQMEDIVLDFDMG+SMLGFSSSLLQRKRSCSEFSPENLLK T
Sbjct: 361 KPKNAVVLGGYQMEDIVLDFDMGSSMLGFSSSLLQRKRSCSEFSPENLLKST 410

BLAST of Lcy01g001990 vs. NCBI nr
Match: KAG6593913.1 (putative aspartic proteinase GIP2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 683.7 bits (1763), Expect = 9.7e-193
Identity = 364/413 (88.14%), Postives = 381/413 (92.25%), Query Frame = 0

Query: 1   MAVPQYPSLLLLL-CFFTLFHGGTFSLVIPVTKDPVTDQYLAAVHHGSPIKPTHLAVDLG 60
           MAVPQ  SLLLLL CFF+ FHG TFSLVIPVTKD VT+QYLA+V+HGSPI+P HLAVDLG
Sbjct: 1   MAVPQSHSLLLLLICFFSFFHGRTFSLVIPVTKDSVTNQYLASVYHGSPIRPIHLAVDLG 60

Query: 61  GPSLWMACGGSSSSRPIPRRSIQCVAAAGGGGGSGSGGGGACDVVAGNPFGALVGKAVLV 120
           GPSLWMACG S+SSR I  RSIQCVAA GGG  SGS GGGAC+VVAGNPFGALVGKA+LV
Sbjct: 61  GPSLWMACGSSTSSRTIQSRSIQCVAATGGGSRSGS-GGGACEVVAGNPFGALVGKALLV 120

Query: 121 EDTVAVRSFDRSTAAVIVAVHSCAPRFLLQGLAKSARGILGLGRNQNSLPAQIATELGFH 180
           EDTVAVR+ +RS AAVIVAVHSCAPRFLLQGLAKSA+GILGLGRNQ SLPAQIATELGFH
Sbjct: 121 EDTVAVRALERSAAAVIVAVHSCAPRFLLQGLAKSAKGILGLGRNQISLPAQIATELGFH 180

Query: 181 RRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDGSQSPEYFINVKAIKV 240
           RRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTK IDGSQS EYFINVKAIKV
Sbjct: 181 RRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKIIDGSQSAEYFINVKAIKV 240

Query: 241 DGNRLDLNKSLLDLDGGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIKEVAP 300
           DGNRLDLNKSLLDLDG +G GGTRLSTVVPYTALESSIFKSLTAAFR AA AMN+KEVAP
Sbjct: 241 DGNRLDLNKSLLDLDGEDG-GGTRLSTVVPYTALESSIFKSLTAAFREAARAMNMKEVAP 300

Query: 301 VAPFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDETFCLGFVDGG 360
           VA FEACF++ENME+TA GPKVP IELILQSEMV WKI GRNSMVKVNDETFCL  VDGG
Sbjct: 301 VASFEACFSSENMEITAAGPKVPMIELILQSEMVRWKIDGRNSMVKVNDETFCLAIVDGG 360

Query: 361 LKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLKKT 413
           LKPKNAVVLGGYQMEDIVLDFDMG+SMLGFSSSLLQRKRSCSEFSPENLLK T
Sbjct: 361 LKPKNAVVLGGYQMEDIVLDFDMGSSMLGFSSSLLQRKRSCSEFSPENLLKST 411

BLAST of Lcy01g001990 vs. NCBI nr
Match: XP_023515015.1 (basic 7S globulin-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 682.6 bits (1760), Expect = 2.2e-192
Identity = 362/412 (87.86%), Postives = 379/412 (91.99%), Query Frame = 0

Query: 1   MAVPQYPSLLLLLCFFTLFHGGTFSLVIPVTKDPVTDQYLAAVHHGSPIKPTHLAVDLGG 60
           MAVPQ  SLLLL+CFF+ FHG TFSLVIPVTKD VT+QYLA+V+HGSPIKP  LAVDLGG
Sbjct: 1   MAVPQSHSLLLLICFFSFFHGRTFSLVIPVTKDSVTNQYLASVYHGSPIKPIRLAVDLGG 60

Query: 61  PSLWMACGGSSSSRPIPRRSIQCVAAAGGGGGSGSGGGGACDVVAGNPFGALVGKAVLVE 120
           PSLWMACG S+SSR I  RSIQCVAA GGG  SGS GGGAC+VVAGN FGALVGKA+LVE
Sbjct: 61  PSLWMACGSSTSSRTIQSRSIQCVAATGGGSRSGS-GGGACEVVAGNSFGALVGKALLVE 120

Query: 121 DTVAVRSFDRSTAAVIVAVHSCAPRFLLQGLAKSARGILGLGRNQNSLPAQIATELGFHR 180
           DTVAVR+ +RSTAAVIVAVHSCAPRFLLQGLAKSA+GILGLGRNQ SLPAQIATELGFHR
Sbjct: 121 DTVAVRALERSTAAVIVAVHSCAPRFLLQGLAKSAKGILGLGRNQISLPAQIATELGFHR 180

Query: 181 RFSVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDGSQSPEYFINVKAIKVD 240
           RFSVCLSSTNGVVFPDSGSQDSVYGSEIS+SLTYTPILTK IDGSQS EYFINVKAIKVD
Sbjct: 181 RFSVCLSSTNGVVFPDSGSQDSVYGSEISNSLTYTPILTKIIDGSQSAEYFINVKAIKVD 240

Query: 241 GNRLDLNKSLLDLDGGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIKEVAPV 300
           GNRLDLNKS LDLDG +G GGTRLSTVVPYTALESSIFKSLTAAFR AA AMN+KEVAPV
Sbjct: 241 GNRLDLNKSQLDLDGEDG-GGTRLSTVVPYTALESSIFKSLTAAFREAARAMNMKEVAPV 300

Query: 301 APFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDETFCLGFVDGGL 360
           A FEACFA+ENMEMTA GPKVP IELILQSE+V WKI GRNSMVKVNDETFCL  VDGGL
Sbjct: 301 ASFEACFASENMEMTAAGPKVPMIELILQSELVRWKIDGRNSMVKVNDETFCLAIVDGGL 360

Query: 361 KPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPENLLKKT 413
           KPKNAVVLGGYQMEDIVLDFDMG+SMLGFSSSLLQRKRSCSEFSPENLLK T
Sbjct: 361 KPKNAVVLGGYQMEDIVLDFDMGSSMLGFSSSLLQRKRSCSEFSPENLLKST 410

BLAST of Lcy01g001990 vs. TAIR 10
Match: AT1G03220.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 268.1 bits (684), Expect = 1.2e-71
Identity = 164/404 (40.59%), Postives = 239/404 (59.16%), Query Frame = 0

Query: 25  SLVIPVTKDPVTDQYLAAVHHGSPIKPTHLAVDLGGPSLWMAC--GGSSSSRPIPRRSIQ 84
           +L++PVTKD  T QY   ++  +P+ P  +  DLGG  LW+ C  G  SS+   PR    
Sbjct: 30  ALLLPVTKDQSTLQYTTVINQRTPLVPASVVFDLGGRELWVDCDKGYVSSTYQSPR---- 89

Query: 85  CVAAAGGGGGSGSGG--------GGACDVVAGNPFGALVGKAVLVE---DTVAVRSFDRS 144
           C +A     GS S G        G + +   G P   + G A   E   D V+++S + S
Sbjct: 90  CNSAVCSRAGSTSCGTCFSPPRPGCSNNTCGGIPDNTVTGTATSGEFALDVVSIQSTNGS 149

Query: 145 TAAVIV----AVHSCAPRFLLQGLAKSARGILGLGRNQNSLPAQIATELGFHRRFSVCLS 204
               +V     +  C   FLL+GLAK   G+ G+GR+   LP+Q A    FHR+F+VCL+
Sbjct: 150 NPGRVVKIPNLIFDCGATFLLKGLAKGTVGMAGMGRHNIGLPSQFAAAFSFHRKFAVCLT 209

Query: 205 STNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKI-------DGSQSPEYFINVKAIKVD 264
           S  GV F  +G    + G +I SSL  TP+L   +        G +S EYFI V AI++ 
Sbjct: 210 SGKGVAFFGNGPYVFLPGIQI-SSLQTTPLLINPVSTASAFSQGEKSSEYFIGVTAIQIV 269

Query: 265 GNRLDLNKSLLDLDGGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIKEVAPV 324
              + +N +LL ++   G+GGT++S+V PYT LESSI+ + T+ F   AAA +IK VA V
Sbjct: 270 EKTVPINPTLLKINASTGIGGTKISSVNPYTVLESSIYNAFTSEFVKQAAARSIKRVASV 329

Query: 325 APFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNSMVKVNDETFCLGFVDGGL 384
            PF ACF+ +N+ +T  G  VPEIEL+L S+ V W+I G NSMV V+D+  CLGFVDGG+
Sbjct: 330 KPFGACFSTKNVGVTRLGYAVPEIELVLHSKDVVWRIFGANSMVSVSDDVICLGFVDGGV 389

Query: 385 KPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFS 405
             + +VV+GG+Q+ED +++FD+ ++  GFSS+LL R+ +C+ F+
Sbjct: 390 NARTSVVIGGFQLEDNLIEFDLASNKFGFSSTLLGRQTNCANFN 428

BLAST of Lcy01g001990 vs. TAIR 10
Match: AT1G03230.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 260.8 bits (665), Expect = 1.9e-69
Identity = 168/422 (39.81%), Postives = 243/422 (57.58%), Query Frame = 0

Query: 10  LLLLCFFTLFHGGTFS-----LVIPVTKDPVTDQYLAAVHHGSPIKPTHLAVDLGGPSLW 69
           +LLL  F+L      S     L++PVTKDP T QY   ++  +P+ P  +  DLGG   W
Sbjct: 11  VLLLSIFSLSSSAQPSFRPKALLLPVTKDPSTLQYTTVINQRTPLVPASVVFDLGGREFW 70

Query: 70  MACGGSSSSRPIPRRSIQCVAAAGGGGGSGSGG--------GGACDVVAGNPFGALVGKA 129
           + C     S     RS +C +A     GS + G        G + +     P  ++ G A
Sbjct: 71  VDCDQGYVSTTY--RSPRCNSAVCSRAGSIACGTCFSPPRPGCSNNTCGAFPDNSITGWA 130

Query: 130 VLVE---DTVAVRSFDRSTAAVIV----AVHSCAPRFLLQGLAKSARGILGLGRNQNSLP 189
              E   D V+++S + S     V     + SC    LL+GLAK A G+ G+GR+   LP
Sbjct: 131 TSGEFALDVVSIQSTNGSNPGRFVKIPNLIFSCGSTSLLKGLAKGAVGMAGMGRHNIGLP 190

Query: 190 AQIATELGFHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKK-------I 249
            Q A    F+R+F+VCL+S  GV F  +G    + G +I S L  TP+L           
Sbjct: 191 LQFAAAFSFNRKFAVCLTSGRGVAFFGNGPYVFLPGIQI-SRLQKTPLLINPGTTVFEFS 250

Query: 250 DGSQSPEYFINVKAIKVDGNRLDLNKSLLDLDGGEGVGGTRLSTVVPYTALESSIFKSLT 309
            G +SPEYFI V AIK+    L ++ +LL ++   G+GGT++S+V PYT LESSI+K+ T
Sbjct: 251 KGEKSPEYFIGVTAIKIVEKTLPIDPTLLKINASTGIGGTKISSVNPYTVLESSIYKAFT 310

Query: 310 AAFRAAAAAMNIKEVAPVAPFEACFAAENMEMTATGPKVPEIELILQSEMVGWKIAGRNS 369
           + F   AAA +IK VA V PF ACF+ +N+ +T  G  VPEI+L+L S+ V W+I G NS
Sbjct: 311 SEFIRQAAARSIKRVASVKPFGACFSTKNVGVTRLGYAVPEIQLVLHSKDVVWRIFGANS 370

Query: 370 MVKVNDETFCLGFVDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSE 405
           MV V+D+  CLGFVDGG+ P  +VV+GG+Q+ED +++FD+ ++  GFSS+LL R+ +C+ 
Sbjct: 371 MVSVSDDVICLGFVDGGVNPGASVVIGGFQLEDNLIEFDLASNKFGFSSTLLGRQTNCAN 429

BLAST of Lcy01g001990 vs. TAIR 10
Match: AT5G19120.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 217.2 bits (552), Expect = 2.4e-56
Identity = 154/385 (40.00%), Postives = 200/385 (51.95%), Query Frame = 0

Query: 26  LVIPVTKDPVTDQYLAAVHHGSPIKPTHLAVDLGGPSLWMACGG---SSSSRPIPRRSIQ 85
           +V PV KD  T QYLA +  G    P  L VDL G  LW  C     SSS   I   S  
Sbjct: 32  VVFPVVKDLPTGQYLAQIRLGDSPDPVKLVVDLAGSILWFDCSSRHVSSSRNLISGSSSG 91

Query: 86  CVAAAGG------GGGSGSGGGGACDVVAGNPFGALVGKAVLVEDTVAVRSFDRSTAAVI 145
           C+ A  G         S       C+++  N    +  +  L  D ++V S   ++   +
Sbjct: 92  CLKAKVGNERVSSSSSSRKDQNADCELLVKNDAFGITARGELFSDVMSVGSV--TSPGTV 151

Query: 146 VAVHSCAPRFLLQGLAKSARGILGLGRNQNSLPAQIATELGFHRRFSVCLSSTNGVVFPD 205
             + +C P +LL+GLA  A+G++GLGR Q SLP+Q+A E    RR +V LS  NGVV   
Sbjct: 152 DLLFACTPPWLLRGLASGAQGVMGLGRAQISLPSQLAAETNERRRLTVYLSPLNGVV--S 211

Query: 206 SGSQDSVYGSEISSSLTYTPILTKKIDGSQSPEYFINVKAIKVDGNRLDLNKSLLDLDGG 265
           + S + V+G   S SL YTP+LT       S  Y INVK+I+V+G +L +          
Sbjct: 212 TSSVEEVFGVAASRSLVYTPLLT-----GSSGNYVINVKSIRVNGEKLSV---------- 271

Query: 266 EGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAMNIKEVAPVAPFEACFAAENMEMTA 325
           EG     LSTVVPYT LESSI+K    A+  A AA     V PVAPF  CF ++      
Sbjct: 272 EGPLAVELSTVVPYTILESSIYKVFAEAY--AKAAGEATSVPPVAPFGLCFTSD------ 331

Query: 326 TGPKVPEIELILQSEMVGWKIAGRNSMVKVNDETFCLGFVDGGLKPKNAVVLGGYQMEDI 385
                P ++L LQSEMV W+I G+N MV V     C G VDGG    N +V+GG Q+E  
Sbjct: 332 --VDFPAVDLALQSEMVRWRIHGKNLMVDVGGGVRCSGIVDGGSSRVNPIVMGGLQLEGF 383

Query: 386 VLDFDMGTSMLGFSSSLLQRKRSCS 402
           +LDFD+G SM+GF     QR RS S
Sbjct: 392 ILDFDLGNSMMGFG----QRTRSDS 383

BLAST of Lcy01g001990 vs. TAIR 10
Match: AT5G19110.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 170.6 bits (431), Expect = 2.6e-42
Identity = 138/412 (33.50%), Postives = 199/412 (48.30%), Query Frame = 0

Query: 8   SLLLLLCFFTLFHG----GTFSLVIPVTKDPVTDQYLAAVHHGSPIK-PTHLAVDLGGPS 67
           SL  LL F ++F           ++P+TK   T+ +    + GS  K P +L +DLG   
Sbjct: 4   SLTRLLVFLSIFAAIALKSNSQYLLPITKHEPTNLFYTTFNVGSAAKSPVNLLLDLGTNL 63

Query: 68  LWMAC---GGSSSSRPIPRRSIQCVAAAGGGGGSGSGGGGACDVVAGNPFG---ALVGKA 127
            W+ C      SS R +  +S  C +  G G       G +C     NP G    + G+ 
Sbjct: 64  TWLDCRKLKSLSSLRLVTCQSSTCKSIPGNG-----CAGKSCLYKQPNPLGQNPVVTGR- 123

Query: 128 VLVEDTVAVRSFDRSTAAVIVAVH----SCAPRFLLQGLAKSARGILGLGRNQNSLPAQI 187
            +V+D  ++ + D       V+V     SCA    LQGL     G+L L    +S   Q+
Sbjct: 124 -VVQDRASLYTTDGGKFLSQVSVRHFTFSCAGEKALQGLPPPVDGVLALSPGSSSFTKQV 183

Query: 188 ATELGFHRRFSVCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDGSQSPEYFI 247
            +      +FS+CL S+    F  +G     +    +SS    P     I G+ S +Y I
Sbjct: 184 TSAFNVIPKFSLCLPSSGTGHFYIAGIH--YFIPPFNSSDNPIPRTLTPIKGTDSGDYLI 243

Query: 248 NVKAIKVDGNRLDLNKSLLDLDGGEGVGGTRLSTVVPYTALESSIFKSLTAAFRAAAAAM 307
            VK+I V G  L LN  LL        GG +LSTVV YT L++ I+ +L  +F   A AM
Sbjct: 244 TVKSIYVGGTALKLNPDLL-------TGGAKLSTVVHYTVLQTDIYNALAQSFTLKAKAM 303

Query: 308 NIKEVAPVAPFEACFAAENMEMTAT-GPKVPEIELILQSEM--VGWKIAGRNSMVKVNDE 367
            I +V  VAPF+ CF +       T GP VP IE+ L   +  V W   G N++VKV + 
Sbjct: 304 GIAKVPSVAPFKHCFDSRTAGKNLTAGPNVPVIEIGLPGRIGEVKWGFYGANTVVKVKET 363

Query: 368 TFCLGFVDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCS 402
             CL F+DGG  PK+ +V+G +Q++D +L+FD   ++L FS SLL    SCS
Sbjct: 364 VMCLAFIDGGKTPKDLMVIGTHQLQDHMLEFDFSGTVLAFSESLLLHNTSCS 399

BLAST of Lcy01g001990 vs. TAIR 10
Match: AT5G19100.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 138.7 bits (348), Expect = 1.1e-32
Identity = 89/250 (35.60%), Postives = 133/250 (53.20%), Query Frame = 0

Query: 159 LGLGRNQNSLPAQIATELGFHRRFSVCLSST------NGVVFPDSGSQDSV-YGSEISSS 218
           +GL     S+P+Q+ +      + ++CL ST      NG ++   G    + Y  ++S  
Sbjct: 160 IGLANTHLSIPSQLISMYQLPHKIALCLPSTERSQSHNGDLWIGKGEYYYLPYDKDVSKI 219

Query: 219 LTYTPILTKKIDGSQSPEYFINVKAIKVDGNRLDLNKSLLDLDGGEGVGGTRLSTVVPYT 278
              TP+    I   +S EY I+VK+I++    + +             G T++ST+ PYT
Sbjct: 220 FASTPL----IGNGKSGEYLIDVKSIQIGAKTVPI-----------PYGATKISTLAPYT 279

Query: 279 ALESSIFKSLTAAFRAAAAAMNIKEVAPVAPFEACFAAENMEMTATGPKVPEIELILQSE 338
             ++S++K+L  AF      + I +   V PF ACF +        G  VP I+L+L S 
Sbjct: 280 VFQTSLYKALLTAF---TENIKIAKAPAVKPFGACFYSNG------GRGVPVIDLVL-SG 339

Query: 339 MVGWKIAGRNSMVKVNDETFCLGFVDGGLKPKNAVVLGGYQMEDIVLDFDMGTSMLGFSS 398
              W+I G NS+VKVN    CLGFVDGG+KPK  +V+GG+QMED +++FD+  S   FSS
Sbjct: 340 GAKWRIYGSNSLVKVNKNVVCLGFVDGGVKPKYPIVIGGFQMEDNLVEFDLEASKFSFSS 384

Query: 399 SLLQRKRSCS 402
           SLL    SCS
Sbjct: 400 SLLLHNTSCS 384

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P0DO213.7e-7040.25Probable aspartic proteinase GIP2 OS=Nicotiana benthamiana OX=4100 GN=GIP2 PE=1 ... [more]
I1JNS61.5e-5836.30Probable aspartic proteinase GIP1 OS=Glycine max OX=3847 GN=GIP1 PE=1 SV=2[more]
P829523.6e-4934.67Gamma conglutin 1 OS=Prunus dulcis OX=3755 GN=Cgamma1 PE=1 SV=2[more]
Q423697.1e-3731.20Gamma conglutin 1 OS=Lupinus angustifolius OX=3871 GN=LOC109345795 PE=1 SV=1[more]
Q9FSH91.6e-3630.07Gamma conglutin 1 OS=Lupinus albus OX=3870 GN=Cgamma PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1EQJ33.3e-19488.35basic 7S globulin-like OS=Cucurbita moschata OX=3662 GN=LOC111436893 PE=4 SV=1[more]
A0A0A0KHJ83.1e-19286.06Basic 7S globulin 2 small subunit OS=Cucumis sativus OX=3659 GN=Csa_6G237600 PE=... [more]
A0A6J1H4R88.9e-19286.30basic 7S globulin-like OS=Cucurbita moschata OX=3662 GN=LOC111460386 PE=4 SV=1[more]
A0A6J1KM091.3e-19087.14basic 7S globulin-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111494504 PE... [more]
A0A6J1KHR11.3e-19087.14basic 7S globulin-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111494504 PE... [more]
Match NameE-valueIdentityDescription
XP_022930452.16.7e-19488.35basic 7S globulin-like [Cucurbita moschata][more]
KAG7026255.11.2e-19388.62Basic 7S globulin, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023515016.19.7e-19388.11basic 7S globulin-like isoform X2 [Cucurbita pepo subsp. pepo][more]
KAG6593913.19.7e-19388.14putative aspartic proteinase GIP2, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023515015.12.2e-19287.86basic 7S globulin-like isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT1G03220.11.2e-7140.59Eukaryotic aspartyl protease family protein [more]
AT1G03230.11.9e-6939.81Eukaryotic aspartyl protease family protein [more]
AT5G19120.12.4e-5640.00Eukaryotic aspartyl protease family protein [more]
AT5G19110.12.6e-4233.50Eukaryotic aspartyl protease family protein [more]
AT5G19100.11.1e-3235.60Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 229..390
e-value: 1.3E-52
score: 177.8
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 23..195
e-value: 6.2E-31
score: 109.8
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 203..406
e-value: 4.4E-60
score: 204.6
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 30..394
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 39..190
e-value: 9.3E-24
score: 84.6
IPR001461Aspartic peptidase A1 familyPANTHERPTHR47965ASPARTYL PROTEASE-RELATEDcoord: 9..403
NoneNo IPR availablePANTHERPTHR47965:SF46BASIC 7S GLOBULIN 2 SMALL SUBUNITcoord: 9..403
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 39..390
score: 17.44318

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy01g001990.1Lcy01g001990.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity