Lag0041646 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0041646
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRVT_3 domain-containing protein
Locationchr13: 22926674 .. 22927123 (-)
RNA-Seq ExpressionLag0041646
SyntenyLag0041646
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCATAATCAACATGACAAGCATGAGGTTCATGTTTCTACTGATGCAACAGTGCATCCTCAAGCTTATGGGTCCGGGTATGGGGTGTTAATGACAGCAAAAGATGGAAACATTATGGGAGCTATGTCAAAATTTGATCTGACTTCGTTCTCCCTATTAGCGGCAGAAGTTCATGCCCTCCTGCATGGTATCAGATTATTATATCACCTACAACATGAAGGTGTTTCACTTTCATCGGACTCCTTAATAGTTGTGCAAATGATTAATGGGGAACACCAATCTTCATCAGATGTGTACCATTGGATAGTGCAAATCCAGTGTTTGAGCAAGTCATTTTCATCTTTTAGGTGCCATTTTATTCCTTGGAATAGAAACATTCGTGCAAACGTTTTGGCTAGAAGAGCATTAGCTCATATAAACTCTATTTTGTGGTTATCTAATTTTCCATAA

mRNA sequence

ATGCATAATCAACATGACAAGCATGAGGTTCATGTTTCTACTGATGCAACAGTGCATCCTCAAGCTTATGGGTCCGGGTATGGGGTGTTAATGACAGCAAAAGATGGAAACATTATGGGAGCTATGTCAAAATTTGATCTGACTTCGTTCTCCCTATTAGCGGCAGAAGTTCATGCCCTCCTGCATGGTATCAGATTATTATATCACCTACAACATGAAGGTGTTTCACTTTCATCGGACTCCTTAATAGTTGTGCAAATGATTAATGGGGAACACCAATCTTCATCAGATGTGTACCATTGGATAGTGCAAATCCAGTGTTTGAGCAAGTCATTTTCATCTTTTAGGTGCCATTTTATTCCTTGGAATAGAAACATTCGTGCAAACGTTTTGGCTAGAAGAGCATTAGCTCATATAAACTCTATTTTGTGGTTATCTAATTTTCCATAA

Coding sequence (CDS)

ATGCATAATCAACATGACAAGCATGAGGTTCATGTTTCTACTGATGCAACAGTGCATCCTCAAGCTTATGGGTCCGGGTATGGGGTGTTAATGACAGCAAAAGATGGAAACATTATGGGAGCTATGTCAAAATTTGATCTGACTTCGTTCTCCCTATTAGCGGCAGAAGTTCATGCCCTCCTGCATGGTATCAGATTATTATATCACCTACAACATGAAGGTGTTTCACTTTCATCGGACTCCTTAATAGTTGTGCAAATGATTAATGGGGAACACCAATCTTCATCAGATGTGTACCATTGGATAGTGCAAATCCAGTGTTTGAGCAAGTCATTTTCATCTTTTAGGTGCCATTTTATTCCTTGGAATAGAAACATTCGTGCAAACGTTTTGGCTAGAAGAGCATTAGCTCATATAAACTCTATTTTGTGGTTATCTAATTTTCCATAA

Protein sequence

MHNQHDKHEVHVSTDATVHPQAYGSGYGVLMTAKDGNIMGAMSKFDLTSFSLLAAEVHALLHGIRLLYHLQHEGVSLSSDSLIVVQMINGEHQSSSDVYHWIVQIQCLSKSFSSFRCHFIPWNRNIRANVLARRALAHINSILWLSNFP
Homology
BLAST of Lag0041646 vs. NCBI nr
Match: XP_015383853.1 (uncharacterized protein LOC107176237 [Citrus sinensis])

HSP 1 Score: 63.9 bits (154), Expect = 1.3e-06
Identity = 41/138 (29.71%), Postives = 71/138 (51.45%), Query Frame = 0

Query: 12  VSTDATVHPQAYGSGYGVLMTAKDGNIMGAMSKFDLTSFSLLAAEVHALLHGIRLLYHLQ 71
           V+ DA V+ +   +G GV++      +  A  K  L +  +  AE  A+  G+ +     
Sbjct: 343 VNVDAAVNFERQKAGLGVVIRDSCNKVKVAAVKTTLFTGDVKTAEAEAVEWGLVVAKSAA 402

Query: 72  HEGVSLSSDSLIVVQMINGEHQSSSDVYHWIVQIQCLSKSFSSFRCHFIPWNRNIRANVL 131
            + + + SD   VV++IN +  S ++V   I +IQ LSK F +F C+  P + N  A+ L
Sbjct: 403 FQCIMVESDCQEVVKLINNKEGSRTEVMWVISEIQSLSKDFQNFSCYHTPRSCNTHAHSL 462

Query: 132 ARRALAHINSILWLSNFP 150
           A+ AL +  +++WL   P
Sbjct: 463 AKLALRNNETVVWLEPIP 480

BLAST of Lag0041646 vs. NCBI nr
Match: GAY61101.1 (hypothetical protein CUMW_207140, partial [Citrus unshiu])

HSP 1 Score: 62.8 bits (151), Expect = 2.9e-06
Identity = 37/140 (26.43%), Postives = 71/140 (50.71%), Query Frame = 0

Query: 10  VHVSTDATVHPQAYGSGYGVLMTAKDGNIMGAMSKFDLTSFSLLAAEVHALLHGIRLLYH 69
           V ++TDA  + +   +G G ++  ++G +     K      S+  AE  A+  G+++   
Sbjct: 311 VKINTDAATNSEKNLAGLGAVIRDENGQVTATAIKVSKFHGSVAYAEAEAMEWGLQVAKD 370

Query: 70  LQHEGVSLSSDSLIVVQMINGEHQSSSDVYHWIVQIQCLSKSFSSFRCHFIPWNRNIRAN 129
              + V + SDS  VV ++N    S S++Y  +++IQ L +SF    C +   + N  A+
Sbjct: 371 AHVKDVIMESDSQEVVSLVNNRQGSRSEIYWVVLEIQKLKESFDHVSCVYTHRSCNAIAH 430

Query: 130 VLARRALAHINSILWLSNFP 150
            LA+ AL    +++W  ++P
Sbjct: 431 SLAKLALEKCETVVWKGSYP 450

BLAST of Lag0041646 vs. NCBI nr
Match: GAV61531.1 (RVT_3 domain-containing protein [Cephalotus follicularis])

HSP 1 Score: 62.8 bits (151), Expect = 2.9e-06
Identity = 37/115 (32.17%), Postives = 69/115 (60.00%), Query Frame = 0

Query: 24  GSGYGVLMTAKDGNIMGAMSKFDLTSFSLLAAEVHALLHGIRLLYHLQHEGVSLSSDSLI 83
           GSG G+++T+ DG  +    +F   + +   AE  AL+ G+++  HL+ + +  SSDS +
Sbjct: 25  GSGTGLVLTSPDGWTLEYALRFGFKATN-NEAEWEALIVGLKISKHLEVQKIEASSDSQL 84

Query: 84  VVQMINGEHQSSSD-VYHWIVQIQCLSKSFSSFRCHFIPWNRNIRANVLARRALA 138
           VV ++NGE+++  D +  ++  +Q L  +F  FR   +P  +N+RA+ L++ A A
Sbjct: 85  VVGLVNGEYEAREDSMAKYLTHVQSLKSAFQVFRVLKVPRAKNVRADQLSKLATA 138

BLAST of Lag0041646 vs. NCBI nr
Match: XP_015381856.1 (uncharacterized protein LOC107175052 [Citrus sinensis])

HSP 1 Score: 62.0 bits (149), Expect = 5.0e-06
Identity = 38/140 (27.14%), Postives = 75/140 (53.57%), Query Frame = 0

Query: 10  VHVSTDATVHPQAYGSGYGVLMTAKDGNIMGAMSKFDLTSFSLLAAEVHALLHGIRLLYH 69
           + ++ DA +  +   +G GV++    G I+ A  K +  S ++  AE  A+L G+++   
Sbjct: 121 IKINVDAAMDEENRLAGLGVVIRNFKGEIIAAAVKTEKNSGNVEMAEAKAVLWGLQVAIM 180

Query: 70  LQHEGVSLSSDSLIVVQMINGEHQSSSDVYHWIVQIQCLSKSFSSFRCHFIPWNRNIRAN 129
            +   V +  DS  V+++IN +  + +++   I  I    KSF +F+   +  + N+ A+
Sbjct: 181 AEATQVIVEYDSKGVIELINNKMGTLTEIVWVIFDILETKKSFQNFKAQHVSRSCNVSAH 240

Query: 130 VLARRALAHINSILWLSNFP 150
            LA+ AL  ++S +WL+ FP
Sbjct: 241 SLAKLALRKLDSFVWLNEFP 260

BLAST of Lag0041646 vs. NCBI nr
Match: XP_015385025.1 (uncharacterized protein LOC107176703 [Citrus sinensis])

HSP 1 Score: 61.6 bits (148), Expect = 6.5e-06
Identity = 38/138 (27.54%), Postives = 64/138 (46.38%), Query Frame = 0

Query: 12  VSTDATVHPQAYGSGYGVLMTAKDGNIMGAMSKFDLTSFSLLAAEVHALLHGIRLLYHLQ 71
           V+ DA VH +   +G GV++    G I+ A  K      ++ AAE  A+  G+ +    +
Sbjct: 115 VNVDAAVHTEQQLTGLGVVIRNPQGQIIVAAIKSTKFQDNVTAAEAEAVKWGLEIALEAR 174

Query: 72  HEGVSLSSDSLIVVQMINGEHQSSSDVYHWIVQIQCLSKSFSSFRCHFIPWNRNIRANVL 131
              V + +D + V  + N +  S  ++   I  I    + F S     +P   N  A+ L
Sbjct: 175 LAAVIIETDCIEVANLANSKTSSKKEIMWTISDIHSYKEKFQSLSIQHVPRCCNSCAHSL 234

Query: 132 ARRALAHINSILWLSNFP 150
           A+RAL  + ++ W  NFP
Sbjct: 235 AKRALRSLETVTWKDNFP 252

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_015383853.11.3e-0629.71uncharacterized protein LOC107176237 [Citrus sinensis][more]
GAY61101.12.9e-0626.43hypothetical protein CUMW_207140, partial [Citrus unshiu][more]
GAV61531.12.9e-0632.17RVT_3 domain-containing protein [Cephalotus follicularis][more]
XP_015381856.15.0e-0627.14uncharacterized protein LOC107175052 [Citrus sinensis][more]
XP_015385025.16.5e-0627.54uncharacterized protein LOC107176703 [Citrus sinensis][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 14..135
e-value: 8.7E-19
score: 67.5
IPR002156Ribonuclease H domainPROSITEPS50879RNASE_Hcoord: 26..137
score: 8.615859
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 8..141
e-value: 6.8E-15
score: 57.2
NoneNo IPR availablePANTHERPTHR33033:SF67SUBFAMILY NOT NAMEDcoord: 9..142
NoneNo IPR availablePANTHERPTHR33033POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 9..142
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 14..133
e-value: 1.49029E-13
score: 61.176
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 9..133

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0041646.1Lag0041646.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity