Lag0041645 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0041645
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	GDSL esterase/lipase 1-like
Location	chr13: 22925594 .. 22926224 (+)
RNA-Seq Expression	Lag0041645
Synteny	Lag0041645

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGAAAGCAGACAAGAACGGAAACTGTTTAGATGAAGCTTCCTCATTATCAAGGCTCAACAACAAGCTCATGCCTGTTGCCCTAAACAACCTTGCCATTCAACTCAAAGGCTTCAAATACACCATATTTGATTCCTACACTACGCTTCTCCAAAGAATGCAAAACCCTTCAAAATATGGTAACCAAATTCTCCTCTACTTTTCTTTATATACTTCCTTTTTTTAAGTACAAAAAACAATGGTATGAAGTGTATGATTTTGACTCACACATCCATGACATTTAGAAAACTTTCTTTCATATTTAAATTATTAAGTTATTGAGAATATAGTATACTCAAACTAAAAACACAAGTGATGATGTGTGTGATGTAGGCTTCAAGGAAGGGATAACAGCATGTTGTGGGAGTAAAAGGTTCAGAGGACTGTATAACTGTGGAGGGAAGAGATTTGTGAAAGAGTACGAGCTGTGCGAAAATCCCAACGACTATTTTTTTTTTGATTCCTATCATCCTACTGAAAAAGGTTACGAGCAGCTTGCAAAGTTAATGTGGAGTGGAGATACTCAAGTCATGAAGCCTTATAACCTCAAACAACTCTTTGAATATGAGACCTCGTTGGCTTCTGAGTGA

mRNA sequence

ATGTTGAAAGCAGACAAGAACGGAAACTGTTTAGATGAAGCTTCCTCATTATCAAGGCTCAACAACAAGCTCATGCCTGTTGCCCTAAACAACCTTGCCATTCAACTCAAAGGCTTCAAATACACCATATTTGATTCCTACACTACGCTTCTCCAAAGAATGCAAAACCCTTCAAAATATGGCTTCAAGGAAGGGATAACAGCATGTTGTGGGAGTAAAAGGTTCAGAGGACTGTATAACTGTGGAGGGAAGAGATTTGTGAAAGAGTACGAGCTGTGCGAAAATCCCAACGACTATTTTTTTTTTGATTCCTATCATCCTACTGAAAAAGGTTACGAGCAGCTTGCAAAGTTAATGTGGAGTGGAGATACTCAAGTCATGAAGCCTTATAACCTCAAACAACTCTTTGAATATGAGACCTCGTTGGCTTCTGAGTGA

Coding sequence (CDS)

ATGTTGAAAGCAGACAAGAACGGAAACTGTTTAGATGAAGCTTCCTCATTATCAAGGCTCAACAACAAGCTCATGCCTGTTGCCCTAAACAACCTTGCCATTCAACTCAAAGGCTTCAAATACACCATATTTGATTCCTACACTACGCTTCTCCAAAGAATGCAAAACCCTTCAAAATATGGCTTCAAGGAAGGGATAACAGCATGTTGTGGGAGTAAAAGGTTCAGAGGACTGTATAACTGTGGAGGGAAGAGATTTGTGAAAGAGTACGAGCTGTGCGAAAATCCCAACGACTATTTTTTTTTTGATTCCTATCATCCTACTGAAAAAGGTTACGAGCAGCTTGCAAAGTTAATGTGGAGTGGAGATACTCAAGTCATGAAGCCTTATAACCTCAAACAACTCTTTGAATATGAGACCTCGTTGGCTTCTGAGTGA

Protein sequence

MLKADKNGNCLDEASSLSRLNNKLMPVALNNLAIQLKGFKYTIFDSYTTLLQRMQNPSKYGFKEGITACCGSKRFRGLYNCGGKRFVKEYELCENPNDYFFFDSYHPTEKGYEQLAKLMWSGDTQVMKPYNLKQLFEYETSLASE

Homology

[Close]

BLAST of Lag0041645 vs. NCBI nr
Match: XP_022146472.1 (GDSL esterase/lipase 1-like [Momordica charantia])

HSP 1 Score: 208.4 bits (529), Expect = 4.2e-50
Identity = 91/143 (63.64%), Postives = 117/143 (81.82%), Query Frame = 0


Query: 1   MLKADKNGNCLDEASSLSRLNNKLMPVALNNLAIQLKGFKYTIFDSYTTLLQRMQNPSKY 60
           + + D++G C +EAS+L+RL+NK++P AL  LAI+LKGFKYT+ D+YT L  R+ NPSKY
Sbjct: 232 LARGDRHGRCWEEASALARLHNKMLPGALKKLAIKLKGFKYTVADTYTLLQNRIDNPSKY 291

Query: 61  GFKEGITACCGSKRFRGLYNCGGKRFVKEYELCENPNDYFFFDSYHPTEKGYEQLAKLMW 120
           GFKEG + CCGS  +RG+Y+CGGKR  KE+ELC+NPN Y FFDSYHP ++ YEQ AK MW
Sbjct: 292 GFKEGKSGCCGSGEYRGIYSCGGKRGQKEFELCKNPNQYLFFDSYHPNQRAYEQFAKHMW 351

Query: 121 SGDTQVMKPYNLKQLFEYETSLA 144
           SGDTQV+ PYNLKQLF+++TS A
Sbjct: 352 SGDTQVINPYNLKQLFQHDTSSA 374

[Close]

BLAST of Lag0041645 vs. NCBI nr
Match: XP_038897092.1 (GDSL lipase-like [Benincasa hispida])

HSP 1 Score: 206.1 bits (523), Expect = 2.1e-49
Identity = 94/143 (65.73%), Postives = 114/143 (79.72%), Query Frame = 0


Query: 1   MLKADKNGNCLDEASSLSRLNNKLMPVALNNLAIQLKGFKYTIFDSYTTLLQRMQNPSKY 60
           ++K   +G+C DEAS+L+RL+NKL+P+AL  LA +L+GFKYT+ D+YT L  R+ NPSKY
Sbjct: 231 LMKKGGHGSCWDEASALTRLHNKLLPIALQKLADKLQGFKYTVADTYTLLQNRIDNPSKY 290

Query: 61  GFKEGITACCGSKRFRGLYNCGGKRFVKEYELCENPNDYFFFDSYHPTEKGYEQLAKLMW 120
           GFKEG  ACCGS   RG+Y+CGG R  KE+ELCENPN+Y FFDSYHP EK YEQ AKLMW
Sbjct: 291 GFKEGKKACCGSGELRGIYSCGGMRGQKEFELCENPNEYLFFDSYHPNEKAYEQFAKLMW 350

Query: 121 SGDTQVMKPYNLKQLFEYETSLA 144
           SGD QV+KPYNLKQLF+   S A
Sbjct: 351 SGDAQVIKPYNLKQLFQCGLSQA 373

[Close]

BLAST of Lag0041645 vs. NCBI nr
Match: XP_008462253.1 (PREDICTED: GDSL esterase/lipase 5-like isoform X1 [Cucumis melo] >KAA0059365.1 GDSL esterase/lipase 5-like isoform X1 [Cucumis melo var. makuwa] >TYK03961.1 GDSL esterase/lipase 5-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 203.4 bits (516), Expect = 1.4e-48
Identity = 92/138 (66.67%), Postives = 111/138 (80.43%), Query Frame = 0


Query: 1   MLKADKNGNCLDEASSLSRLNNKLMPVALNNLAIQLKGFKYTIFDSYTTLLQRMQNPSKY 60
           ++K D +G+CLDEAS+L RL+NKL+  AL  LA +L GFKYTI D+YT L  R+ NPSKY
Sbjct: 231 LMKKDGHGSCLDEASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKY 290

Query: 61  GFKEGITACCGSKRFRGLYNCGGKRFVKEYELCENPNDYFFFDSYHPTEKGYEQLAKLMW 120
           G KEG  ACCGS + RG+Y+CGG R VKE+ELCENPN+Y FFDSYHP E+ YEQ AKLMW
Sbjct: 291 GLKEGKKACCGSGKLRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMW 350

Query: 121 SGDTQVMKPYNLKQLFEY 139
           SGD+QV+ PYNLKQ F+Y
Sbjct: 351 SGDSQVINPYNLKQFFQY 368

[Close]

BLAST of Lag0041645 vs. NCBI nr
Match: XP_004141726.1 (GDSL lipase isoform X1 [Cucumis sativus] >KGN45451.1 hypothetical protein Csa_016520 [Cucumis sativus])

HSP 1 Score: 203.0 bits (515), Expect = 1.8e-48
Identity = 92/139 (66.19%), Postives = 113/139 (81.29%), Query Frame = 0


Query: 3   KADKNGNCLDEASSLSRLNNKLMPVALNNLAIQLKGFKYTIFDSYTTLLQRMQNPSKYGF 62
           KA  +G+C DE S+L RL+NKL+P AL  LA +L+GFKYT+ D+YT L  R+ NPSKYGF
Sbjct: 234 KAGGHGSCWDEPSALVRLHNKLLPGALQKLADKLQGFKYTVGDTYTMLQNRIDNPSKYGF 293

Query: 63  KEGITACCGSKRFRGLYNCGGKRFVKEYELCENPNDYFFFDSYHPTEKGYEQLAKLMWSG 122
           KE  TACCGS +FRG+Y+CGG R VKE+ELCENPN+Y FFDSYHP E+ YEQ AKLMWSG
Sbjct: 294 KEEKTACCGSGKFRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSG 353

Query: 123 DTQVMKPYNLKQLFEYETS 142
           D+QV+ PY+LKQ F+Y +S
Sbjct: 354 DSQVINPYSLKQFFQYASS 372

[Close]

BLAST of Lag0041645 vs. NCBI nr
Match: XP_016902174.1 (PREDICTED: GDSL esterase/lipase 5-like [Cucumis melo] >KAA0045893.1 GDSL esterase/lipase 5-like [Cucumis melo var. makuwa])

HSP 1 Score: 202.6 bits (514), Expect = 2.3e-48
Identity = 91/142 (64.08%), Postives = 114/142 (80.28%), Query Frame = 0


Query: 1   MLKADKNGNCLDEASSLSRLNNKLMPVALNNLAIQLKGFKYTIFDSYTTLLQRMQNPSKY 60
           MLK + +G C++EASS+  L+NKL+P+AL +LA QL GFKY   D+   LLQR+QNPSKY
Sbjct: 229 MLKGEGHGKCVEEASSIVNLHNKLLPIALQSLATQLNGFKYAFADANNLLLQRIQNPSKY 288

Query: 61  GFKEGITACCGSKRFRGLYNCGGKRFVKEYELCENPNDYFFFDSYHPTEKGYEQLAKLMW 120
           GFKE  TACCGS  +RG+Y+CGGKR  KE+++CE+P  Y FFDSYHP +K YEQLA+LMW
Sbjct: 289 GFKEVETACCGSGEYRGIYSCGGKRGEKEFKICEDPTKYLFFDSYHPNQKAYEQLARLMW 348

Query: 121 SGDTQVMKPYNLKQLFEYETSL 143
           SGD QV+KPYNLKQLF+Y + L
Sbjct: 349 SGDEQVIKPYNLKQLFQYGSPL 370

[Close]

BLAST of Lag0041645 vs. ExPASy Swiss-Prot
Match: Q9SSA7 (GDSL esterase/lipase 5 OS=Arabidopsis thaliana OX=3702 GN=GLIP5 PE=2 SV=2)

HSP 1 Score: 148.3 bits (373), Expect = 6.8e-35
Identity = 67/141 (47.52%), Postives = 100/141 (70.92%), Query Frame = 0


Query: 1   MLKADKNGNCLDEASSLSRLNNKLMPVALNNLAIQLKGFKYTIFDSYTTLLQRMQNPSKY 60
           +L+   + +CL +AS L+ ++N+ +   L  +  Q+KGFK+++FD   +L  RMQ+PSK+
Sbjct: 243 ILQPKNDDSCLRDASRLASMHNRALTNLLFQMQRQVKGFKFSLFDMNKSLRLRMQHPSKF 302

Query: 61  GFKEGITACCGSKRFRGLYNCGGKRFVKEYELCENPNDYFFFDSYHPTEKGYEQLAKLMW 120
           GFKEG  ACCG+ ++RG+++CGGKR VKEY+LCENP DY F+DS H T+  Y Q A L+W
Sbjct: 303 GFKEGEEACCGTGKWRGVFSCGGKRIVKEYQLCENPKDYIFWDSLHLTQNTYNQFANLIW 362

Query: 121 SG----DTQVMKPYNLKQLFE 138
           +G    D+ V+ PYN+  LF+
Sbjct: 363 NGGHMSDSLVVGPYNINNLFQ 383

[Close]

BLAST of Lag0041645 vs. ExPASy Swiss-Prot
Match: Q9SYF0 (GDSL esterase/lipase 2 OS=Arabidopsis thaliana OX=3702 GN=GLIP2 PE=2 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 2.2e-33
Identity = 68/133 (51.13%), Postives = 87/133 (65.41%), Query Frame = 0


Query: 6   KNGNCLDEASSLSRLNNKLMPVALNNLAIQLKGFKYTIFDSYTTLLQRMQNPSKYGFKEG 65
           K G C    + L  L+N+ +   L  L  +L GFKY + D +T+L  RM NPSKYGFKEG
Sbjct: 242 KIGTCFKPVTELINLHNEKLESGLRRLERELSGFKYALHDYHTSLSVRMNNPSKYGFKEG 301

Query: 66  ITACCGSKRFRGLYNCGGKRFVKE-YELCENPNDYFFFDSYHPTEKGYEQLAKLMWSGDT 125
             ACCG+   RG+  CGG+  V + YELCE   DY FFD +H TEK ++Q+A+L+WSG T
Sbjct: 302 KMACCGTGPLRGINTCGGRMGVSQSYELCEKVTDYLFFDHFHLTEKAHQQIAELIWSGPT 361

Query: 126 QVMKPYNLKQLFE 138
            V KPYNL+ LFE
Sbjct: 362 NVTKPYNLQALFE 374

[Close]

BLAST of Lag0041645 vs. ExPASy Swiss-Prot
Match: H6U1I8 (GDSL lipase OS=Tanacetum cinerariifolium OX=118510 GN=GLIP PE=1 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 4.9e-33
Identity = 70/141 (49.65%), Postives = 91/141 (64.54%), Query Frame = 0


Query: 2   LKADKNGN-CLDEASSLSRLNNKLMPVALNNLAIQLKGFKYTIFDSYTTLLQRMQNPSKY 61
           ++A + GN C  E   L+RL+N+     L  L  QL+GF Y  FD  T +L RM+NPSKY
Sbjct: 219 MRAKQPGNTCNTEVDELTRLHNQAFAKRLEQLEKQLEGFVYAKFDLSTAILNRMKNPSKY 278

Query: 62  GFKEGITACCGSKRFRGLYNCGGKRFVKEYELCENPNDYFFFDSYHPTEKGYEQLAKLMW 121
           GFKEG +ACCGS  F G Y+CG    +KE+ LC+N  +YFFFD +HP E    Q A++ W
Sbjct: 279 GFKEGESACCGSGPFGGNYDCGR---IKEFGLCDNATEYFFFDPFHPNELASRQFAEMFW 338

Query: 122 SGDTQVMKPYNLKQLFEYETS 142
            GD+ V +PYNLK LFE + S
Sbjct: 339 DGDSMVTQPYNLKALFEGKPS 356

[Close]

BLAST of Lag0041645 vs. ExPASy Swiss-Prot
Match: Q9FLN0 (GDSL esterase/lipase 1 OS=Arabidopsis thaliana OX=3702 GN=GLIP1 PE=1 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 2.0e-31
Identity = 64/130 (49.23%), Postives = 85/130 (65.38%), Query Frame = 0


Query: 9   NCLDEASSLSRLNNKLMPVALNNLAIQLKGFKYTIFDSYTTLLQRMQNPSKYGFKEGITA 68
           +C    + L  ++N+ +   L  L  +L GFKY + D +T+L +RM +PSKYGFKEG  A
Sbjct: 243 SCFQPVTELINMHNEKLLNGLRRLNHELSGFKYALHDYHTSLSERMNDPSKYGFKEGKKA 302

Query: 69  CCGSKRFRGLYNCGGKRFVKE-YELCENPNDYFFFDSYHPTEKGYEQLAKLMWSGDTQVM 128
           CCGS   RG+  CGG+  + + YELCEN  DY FFD +H TEK   Q+A+L+WSG T + 
Sbjct: 303 CCGSGPLRGINTCGGRMGLSQSYELCENVTDYLFFDPFHLTEKANRQIAELIWSGPTNIT 362

Query: 129 KPYNLKQLFE 138
            PYNLK LFE
Sbjct: 363 GPYNLKALFE 372

[Close]

BLAST of Lag0041645 vs. ExPASy Swiss-Prot
Match: Q9LJP1 (GDSL esterase/lipase 4 OS=Arabidopsis thaliana OX=3702 GN=GLIP4 PE=2 SV=2)

HSP 1 Score: 135.2 bits (339), Expect = 5.9e-31
Identity = 64/137 (46.72%), Postives = 88/137 (64.23%), Query Frame = 0


Query: 1   MLKADKNGNCLDEASSLSRLNNKLMPVALNNLAIQLKGFKYTIFDSYTTLLQRMQNPSKY 60
           ++ + K G+C +  + L  L+N+  P  L  L  +L GFKY + D +T+L QR+ NPS+Y
Sbjct: 239 IINSTKIGSCFEPVTELINLHNQEFPKVLRRLERRLSGFKYALHDFHTSLSQRINNPSRY 298

Query: 61  GFKEGITACCGSKRFRGLYNCGGKRFVKE-YELCENPNDYFFFDSYHPTEKGYEQLAKLM 120
           GFKEG  ACCGS   RG+  CG +    + Y+LCEN +DY FFD  H TE  ++Q+A+L+
Sbjct: 299 GFKEGEMACCGSGPLRGINTCGFRNGPSQGYKLCENADDYVFFDPSHLTETAHQQIAELI 358

Query: 121 WSGDTQVMKPYNLKQLF 137
           WSG   V  PYNLK LF
Sbjct: 359 WSGPPNVTAPYNLKTLF 375

The following BLAST results are available for this feature:

BLAST of Lag0041645 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146472.1	4.2e-50	63.64	GDSL esterase/lipase 1-like [Momordica charantia]	[more]
XP_038897092.1	2.1e-49	65.73	GDSL lipase-like [Benincasa hispida]	[more]
XP_008462253.1	1.4e-48	66.67	PREDICTED: GDSL esterase/lipase 5-like isoform X1 [Cucumis melo] >KAA0059365.1 G...	[more]
XP_004141726.1	1.8e-48	66.19	GDSL lipase isoform X1 [Cucumis sativus] >KGN45451.1 hypothetical protein Csa_01...	[more]
XP_016902174.1	2.3e-48	64.08	PREDICTED: GDSL esterase/lipase 5-like [Cucumis melo] >KAA0045893.1 GDSL esteras...	[more]

BLAST of Lag0041645 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SSA7	6.8e-35	47.52	GDSL esterase/lipase 5 OS=Arabidopsis thaliana OX=3702 GN=GLIP5 PE=2 SV=2	[more]
Q9SYF0	2.2e-33	51.13	GDSL esterase/lipase 2 OS=Arabidopsis thaliana OX=3702 GN=GLIP2 PE=2 SV=1	[more]
H6U1I8	4.9e-33	49.65	GDSL lipase OS=Tanacetum cinerariifolium OX=118510 GN=GLIP PE=1 SV=1	[more]
Q9FLN0	2.0e-31	49.23	GDSL esterase/lipase 1 OS=Arabidopsis thaliana OX=3702 GN=GLIP1 PE=1 SV=1	[more]
Q9LJP1	5.9e-31	46.72	GDSL esterase/lipase 4 OS=Arabidopsis thaliana OX=3702 GN=GLIP4 PE=2 SV=2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036514	SGNH hydrolase superfamily	GENE3D	3.40.50.1110	SGNH hydrolase	coord: 2..124 e-value: 4.6E-24 score: 87.4
IPR001087	GDSL lipase/esterase	PFAM	PF00657	Lipase_GDSL	coord: 4..118 e-value: 7.8E-8 score: 33.1
IPR044552	GDSL esterase/lipase GLIP1-5/GLL25	PANTHER	PTHR45966	GDSL-LIKE LIPASE/ACYLHYDROLASE	coord: 1..137
None	No IPR available	PANTHER	PTHR45966:SF4	GDSL ESTERASE/LIPASE 5	coord: 1..137
None	No IPR available	SUPERFAMILY	52266	SGNH hydrolase	coord: 94..121

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0041645.1	Lag0041645.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0016298	lipase activity
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds