Lag0041638 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0041638
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	GDSL esterase/lipase
Location	chr13: 22550011 .. 22550570 (+)
RNA-Seq Expression	Lag0041638
Synteny	Lag0041638

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCTGTTGCCCTAAACAACCTTGCCATTCAGCTCAAAGGCTTCAAATACACCATCTTTGATTACTACACTACGTTACTCCAAAGAATGCAAAACCCTTCAAAATATGGTAACCAAATTCTCGTCTACTTTTCTTTATATACTTCCTTTTTTTTAAGTACAAGAAACAATGGTATGAAGTTTATGATTTTGAGTCACTCATCCATGACATTTAGAAAACTTTCTTTCATATTTAAATTATTAAGTTATTGAGAATATAGTATACTCTAACTAAAAACACAAGTGATGACGTGTACAATGAAGGTTTCAAGGAAGTGAGAACATCATGTTGTGGGAGTAAAAGGTTCAGAGGATTGTATAACTGTGGAGGGATGAGCTTTTCAAAAGAATACGAGTTGTGCGAAAATCCCAACGACTATTTGTTTTTTGATTCTTATCATCCTACTGAAAAAGGTTACGAGCAACTTGCAAAGTTAATGTGGAGTGGAGATGCTCAAGTCATGAAGCCTTATAACCTCAAAGAACTCTTTGAAGATGAGACCTTGTTGGCTTTTGAGTGA

mRNA sequence

ATGCCTGTTGCCCTAAACAACCTTGCCATTCAGCTCAAAGGCTTCAAATACACCATCTTTGATTACTACACTACGTTACTCCAAAGAATGCAAAACCCTTCAAAATATGGTTTCAAGGAAGTGAGAACATCATGTTGTGGGAGTAAAAGGTTCAGAGGATTGTATAACTGTGGAGGGATGAGCTTTTCAAAAGAATACGAGTTGTGCGAAAATCCCAACGACTATTTGTTTTTTGATTCTTATCATCCTACTGAAAAAGGTTACGAGCAACTTGCAAAGTTAATGTGGAGTGGAGATGCTCAAGTCATGAAGCCTTATAACCTCAAAGAACTCTTTGAAGATGAGACCTTGTTGGCTTTTGAGTGA

Coding sequence (CDS)

ATGCCTGTTGCCCTAAACAACCTTGCCATTCAGCTCAAAGGCTTCAAATACACCATCTTTGATTACTACACTACGTTACTCCAAAGAATGCAAAACCCTTCAAAATATGGTTTCAAGGAAGTGAGAACATCATGTTGTGGGAGTAAAAGGTTCAGAGGATTGTATAACTGTGGAGGGATGAGCTTTTCAAAAGAATACGAGTTGTGCGAAAATCCCAACGACTATTTGTTTTTTGATTCTTATCATCCTACTGAAAAAGGTTACGAGCAACTTGCAAAGTTAATGTGGAGTGGAGATGCTCAAGTCATGAAGCCTTATAACCTCAAAGAACTCTTTGAAGATGAGACCTTGTTGGCTTTTGAGTGA

Protein sequence

MPVALNNLAIQLKGFKYTIFDYYTTLLQRMQNPSKYGFKEVRTSCCGSKRFRGLYNCGGMSFSKEYELCENPNDYLFFDSYHPTEKGYEQLAKLMWSGDAQVMKPYNLKELFEDETLLAFE

Homology

[Close]

BLAST of Lag0041638 vs. NCBI nr
Match: XP_038897092.1 (GDSL lipase-like [Benincasa hispida])

HSP 1 Score: 178.3 bits (451), Expect = 3.9e-41
Identity = 78/113 (69.03%), Postives = 93/113 (82.30%), Query Frame = 0


Query: 1   MPVALNNLAIQLKGFKYTIFDYYTTLLQRMQNPSKYGFKEVRTSCCGSKRFRGLYNCGGM 60
           +P+AL  LA +L+GFKYT+ D YT L  R+ NPSKYGFKE + +CCGS   RG+Y+CGGM
Sbjct: 255 LPIALQKLADKLQGFKYTVADTYTLLQNRIDNPSKYGFKEGKKACCGSGELRGIYSCGGM 314

Query: 61  SFSKEYELCENPNDYLFFDSYHPTEKGYEQLAKLMWSGDAQVMKPYNLKELFE 114
              KE+ELCENPN+YLFFDSYHP EK YEQ AKLMWSGDAQV+KPYNLK+LF+
Sbjct: 315 RGQKEFELCENPNEYLFFDSYHPNEKAYEQFAKLMWSGDAQVIKPYNLKQLFQ 367

[Close]

BLAST of Lag0041638 vs. NCBI nr
Match: XP_004141726.1 (GDSL lipase isoform X1 [Cucumis sativus] >KGN45451.1 hypothetical protein Csa_016520 [Cucumis sativus])

HSP 1 Score: 172.9 bits (437), Expect = 1.6e-39
Identity = 75/113 (66.37%), Postives = 93/113 (82.30%), Query Frame = 0


Query: 1   MPVALNNLAIQLKGFKYTIFDYYTTLLQRMQNPSKYGFKEVRTSCCGSKRFRGLYNCGGM 60
           +P AL  LA +L+GFKYT+ D YT L  R+ NPSKYGFKE +T+CCGS +FRG+Y+CGGM
Sbjct: 256 LPGALQKLADKLQGFKYTVGDTYTMLQNRIDNPSKYGFKEEKTACCGSGKFRGIYSCGGM 315

Query: 61  SFSKEYELCENPNDYLFFDSYHPTEKGYEQLAKLMWSGDAQVMKPYNLKELFE 114
              KE+ELCENPN+YLFFDSYHP E+ YEQ AKLMWSGD+QV+ PY+LK+ F+
Sbjct: 316 RGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQVINPYSLKQFFQ 368

[Close]

BLAST of Lag0041638 vs. NCBI nr
Match: XP_016902174.1 (PREDICTED: GDSL esterase/lipase 5-like [Cucumis melo] >KAA0045893.1 GDSL esterase/lipase 5-like [Cucumis melo var. makuwa])

HSP 1 Score: 172.2 bits (435), Expect = 2.8e-39
Identity = 77/122 (63.11%), Postives = 96/122 (78.69%), Query Frame = 0


Query: 1   MPVALNNLAIQLKGFKYTIFDYYTTLLQRMQNPSKYGFKEVRTSCCGSKRFRGLYNCGGM 60
           +P+AL +LA QL GFKY   D    LLQR+QNPSKYGFKEV T+CCGS  +RG+Y+CGG 
Sbjct: 253 LPIALQSLATQLNGFKYAFADANNLLLQRIQNPSKYGFKEVETACCGSGEYRGIYSCGGK 312

Query: 61  SFSKEYELCENPNDYLFFDSYHPTEKGYEQLAKLMWSGDAQVMKPYNLKELFE-DETLLA 120
              KE+++CE+P  YLFFDSYHP +K YEQLA+LMWSGD QV+KPYNLK+LF+    LLA
Sbjct: 313 RGEKEFKICEDPTKYLFFDSYHPNQKAYEQLARLMWSGDEQVIKPYNLKQLFQYGSPLLA 372

Query: 121 FE 122
           ++
Sbjct: 373 YQ 374

[Close]

BLAST of Lag0041638 vs. NCBI nr
Match: XP_038902952.1 (LOW QUALITY PROTEIN: GDSL lipase-like [Benincasa hispida])

HSP 1 Score: 171.0 bits (432), Expect = 6.2e-39
Identity = 75/113 (66.37%), Postives = 91/113 (80.53%), Query Frame = 0


Query: 1   MPVALNNLAIQLKGFKYTIFDYYTTLLQRMQNPSKYGFKEVRTSCCGSKRFRGLYNCGGM 60
           +P+AL NLAIQL  FKY   D  T LLQR++NP+KYGFKEV T+CCGS  + G+Y+CGG 
Sbjct: 273 LPIALQNLAIQLNDFKYAFADANTLLLQRIRNPTKYGFKEVETACCGSGEYGGIYSCGGK 332

Query: 61  SFSKEYELCENPNDYLFFDSYHPTEKGYEQLAKLMWSGDAQVMKPYNLKELFE 114
              KE++LCE+P  YLFFDSYHP +K YEQLAKLMWSGD QV+KPYNLK+LF+
Sbjct: 333 RGKKEFKLCEDPTQYLFFDSYHPNQKAYEQLAKLMWSGDEQVIKPYNLKQLFQ 385

[Close]

BLAST of Lag0041638 vs. NCBI nr
Match: XP_022146472.1 (GDSL esterase/lipase 1-like [Momordica charantia])

HSP 1 Score: 171.0 bits (432), Expect = 6.2e-39
Identity = 74/116 (63.79%), Postives = 91/116 (78.45%), Query Frame = 0


Query: 1   MPVALNNLAIQLKGFKYTIFDYYTTLLQRMQNPSKYGFKEVRTSCCGSKRFRGLYNCGGM 60
           +P AL  LAI+LKGFKYT+ D YT L  R+ NPSKYGFKE ++ CCGS  +RG+Y+CGG 
Sbjct: 256 LPGALKKLAIKLKGFKYTVADTYTLLQNRIDNPSKYGFKEGKSGCCGSGEYRGIYSCGGK 315

Query: 61  SFSKEYELCENPNDYLFFDSYHPTEKGYEQLAKLMWSGDAQVMKPYNLKELFEDET 117
              KE+ELC+NPN YLFFDSYHP ++ YEQ AK MWSGD QV+ PYNLK+LF+ +T
Sbjct: 316 RGQKEFELCKNPNQYLFFDSYHPNQRAYEQFAKHMWSGDTQVINPYNLKQLFQHDT 371

[Close]

BLAST of Lag0041638 vs. ExPASy Swiss-Prot
Match: Q9SYF0 (GDSL esterase/lipase 2 OS=Arabidopsis thaliana OX=3702 GN=GLIP2 PE=2 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 3.8e-31
Identity = 62/110 (56.36%), Postives = 78/110 (70.91%), Query Frame = 0


Query: 5   LNNLAIQLKGFKYTIFDYYTTLLQRMQNPSKYGFKEVRTSCCGSKRFRGLYNCGG-MSFS 64
           L  L  +L GFKY + DY+T+L  RM NPSKYGFKE + +CCG+   RG+  CGG M  S
Sbjct: 265 LRRLERELSGFKYALHDYHTSLSVRMNNPSKYGFKEGKMACCGTGPLRGINTCGGRMGVS 324

Query: 65  KEYELCENPNDYLFFDSYHPTEKGYEQLAKLMWSGDAQVMKPYNLKELFE 114
           + YELCE   DYLFFD +H TEK ++Q+A+L+WSG   V KPYNL+ LFE
Sbjct: 325 QSYELCEKVTDYLFFDHFHLTEKAHQQIAELIWSGPTNVTKPYNLQALFE 374

[Close]

BLAST of Lag0041638 vs. ExPASy Swiss-Prot
Match: Q9FLN0 (GDSL esterase/lipase 1 OS=Arabidopsis thaliana OX=3702 GN=GLIP1 PE=1 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 6.5e-31
Identity = 62/110 (56.36%), Postives = 77/110 (70.00%), Query Frame = 0


Query: 5   LNNLAIQLKGFKYTIFDYYTTLLQRMQNPSKYGFKEVRTSCCGSKRFRGLYNCGG-MSFS 64
           L  L  +L GFKY + DY+T+L +RM +PSKYGFKE + +CCGS   RG+  CGG M  S
Sbjct: 263 LRRLNHELSGFKYALHDYHTSLSERMNDPSKYGFKEGKKACCGSGPLRGINTCGGRMGLS 322

Query: 65  KEYELCENPNDYLFFDSYHPTEKGYEQLAKLMWSGDAQVMKPYNLKELFE 114
           + YELCEN  DYLFFD +H TEK   Q+A+L+WSG   +  PYNLK LFE
Sbjct: 323 QSYELCENVTDYLFFDPFHLTEKANRQIAELIWSGPTNITGPYNLKALFE 372

[Close]

BLAST of Lag0041638 vs. ExPASy Swiss-Prot
Match: Q9SYF5 (GDSL esterase/lipase 3 OS=Arabidopsis thaliana OX=3702 GN=GLIP3 PE=2 SV=2)

HSP 1 Score: 127.5 bits (319), Expect = 1.0e-28
Identity = 59/113 (52.21%), Postives = 78/113 (69.03%), Query Frame = 0


Query: 2   PVALNNLAIQLKGFKYTIFDYYTTLLQRMQNPSKYGFKEVRTSCCGSKRFRGLYNCGG-M 61
           P  L  L  QL GF+Y + DY+T+L +R+ +PSKYGFKE + +CCGS   RG+  CG  +
Sbjct: 251 PDVLRRLQRQLSGFRYALHDYHTSLSERINSPSKYGFKEGKKACCGSGPLRGINTCGNRI 310

Query: 62  SFSKEYELCENPNDYLFFDSYHPTEKGYEQLAKLMWSGDAQVMKPYNLKELFE 114
             S+ Y LCEN  DYLF+DS H TEK + Q+A+L+W+G   V +PYNLK LFE
Sbjct: 311 GPSQGYGLCENVTDYLFYDSSHLTEKAHRQIAELIWNGPPNVTRPYNLKALFE 363

[Close]

BLAST of Lag0041638 vs. ExPASy Swiss-Prot
Match: Q9SSA7 (GDSL esterase/lipase 5 OS=Arabidopsis thaliana OX=3702 GN=GLIP5 PE=2 SV=2)

HSP 1 Score: 127.1 bits (318), Expect = 1.4e-28
Identity = 58/118 (49.15%), Postives = 82/118 (69.49%), Query Frame = 0


Query: 4   ALNNLAIQL----KGFKYTIFDYYTTLLQRMQNPSKYGFKEVRTSCCGSKRFRGLYNCGG 63
           AL NL  Q+    KGFK+++FD   +L  RMQ+PSK+GFKE   +CCG+ ++RG+++CGG
Sbjct: 266 ALTNLLFQMQRQVKGFKFSLFDMNKSLRLRMQHPSKFGFKEGEEACCGTGKWRGVFSCGG 325

Query: 64  MSFSKEYELCENPNDYLFFDSYHPTEKGYEQLAKLMWSG----DAQVMKPYNLKELFE 114
               KEY+LCENP DY+F+DS H T+  Y Q A L+W+G    D+ V+ PYN+  LF+
Sbjct: 326 KRIVKEYQLCENPKDYIFWDSLHLTQNTYNQFANLIWNGGHMSDSLVVGPYNINNLFQ 383

[Close]

BLAST of Lag0041638 vs. ExPASy Swiss-Prot
Match: H6U1I8 (GDSL lipase OS=Tanacetum cinerariifolium OX=118510 GN=GLIP PE=1 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 2.0e-27
Identity = 57/109 (52.29%), Postives = 72/109 (66.06%), Query Frame = 0


Query: 5   LNNLAIQLKGFKYTIFDYYTTLLQRMQNPSKYGFKEVRTSCCGSKRFRGLYNCGGMSFSK 64
           L  L  QL+GF Y  FD  T +L RM+NPSKYGFKE  ++CCGS  F G Y+CG +   K
Sbjct: 247 LEQLEKQLEGFVYAKFDLSTAILNRMKNPSKYGFKEGESACCGSGPFGGNYDCGRI---K 306

Query: 65  EYELCENPNDYLFFDSYHPTEKGYEQLAKLMWSGDAQVMKPYNLKELFE 114
           E+ LC+N  +Y FFD +HP E    Q A++ W GD+ V +PYNLK LFE
Sbjct: 307 EFGLCDNATEYFFFDPFHPNELASRQFAEMFWDGDSMVTQPYNLKALFE 352

The following BLAST results are available for this feature:

BLAST of Lag0041638 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038897092.1	3.9e-41	69.03	GDSL lipase-like [Benincasa hispida]	[more]
XP_004141726.1	1.6e-39	66.37	GDSL lipase isoform X1 [Cucumis sativus] >KGN45451.1 hypothetical protein Csa_01...	[more]
XP_016902174.1	2.8e-39	63.11	PREDICTED: GDSL esterase/lipase 5-like [Cucumis melo] >KAA0045893.1 GDSL esteras...	[more]
XP_038902952.1	6.2e-39	66.37	LOW QUALITY PROTEIN: GDSL lipase-like [Benincasa hispida]	[more]
XP_022146472.1	6.2e-39	63.79	GDSL esterase/lipase 1-like [Momordica charantia]	[more]

BLAST of Lag0041638 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SYF0	3.8e-31	56.36	GDSL esterase/lipase 2 OS=Arabidopsis thaliana OX=3702 GN=GLIP2 PE=2 SV=1	[more]
Q9FLN0	6.5e-31	56.36	GDSL esterase/lipase 1 OS=Arabidopsis thaliana OX=3702 GN=GLIP1 PE=1 SV=1	[more]
Q9SYF5	1.0e-28	52.21	GDSL esterase/lipase 3 OS=Arabidopsis thaliana OX=3702 GN=GLIP3 PE=2 SV=2	[more]
Q9SSA7	1.4e-28	49.15	GDSL esterase/lipase 5 OS=Arabidopsis thaliana OX=3702 GN=GLIP5 PE=2 SV=2	[more]
H6U1I8	2.0e-27	52.29	GDSL lipase OS=Tanacetum cinerariifolium OX=118510 GN=GLIP PE=1 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036514	SGNH hydrolase superfamily	GENE3D	3.40.50.1110	SGNH hydrolase	coord: 3..100 e-value: 3.6E-21 score: 77.9
IPR001087	GDSL lipase/esterase	PFAM	PF00657	Lipase_GDSL	coord: 9..94 e-value: 2.7E-6 score: 28.0
None	No IPR available	PANTHER	PTHR45966:SF4	GDSL ESTERASE/LIPASE 5	coord: 3..113
None	No IPR available	SUPERFAMILY	52266	SGNH hydrolase	coord: 70..97
IPR044552	GDSL esterase/lipase GLIP1-5/GLL25	PANTHER	PTHR45966	GDSL-LIKE LIPASE/ACYLHYDROLASE	coord: 3..113

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0041638.1	Lag0041638.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0016298	lipase activity
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds