Lag0041495 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0041495
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionNuclear transcription factor Y subunit B
Locationchr13: 18959104 .. 18959454 (+)
RNA-Seq ExpressionLag0041495
SyntenyLag0041495
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAGATGATAAGAAGGAAGAGCTTCAATTGCCGATTGCCAATGTGGGCAGATTAATGAAGAAGATACTTCCTCAAACGGCGAAGATTTCAAAGGCTGCGAAACAGACGATGCAAGAATGTGCGACAGAGTTCATTGGGTTCGTCACAAGTGAAGCTGCAAACAAATGTCACACACAGAATCGTAGGACTCTCAATGGAGACGACATTCATTCGGCTTTTGCTGCTCTTGGCTTGGACAACTATGCACAGGCTTCTGCCATGTACTTGCTCAAGTACAGGCAACTCGAAGCTCACGACAACAACGAAGCTAGTCGAACTCAACGACGACGTCGAAATGAAGACGAGTAA

mRNA sequence

ATGGGAGATGATAAGAAGGAAGAGCTTCAATTGCCGATTGCCAATGTGGGCAGATTAATGAAGAAGATACTTCCTCAAACGGCGAAGATTTCAAAGGCTGCGAAACAGACGATGCAAGAATGTGCGACAGAGTTCATTGGGTTCGTCACAAGTGAAGCTGCAAACAAATGTCACACACAGAATCGTAGGACTCTCAATGGAGACGACATTCATTCGGCTTTTGCTGCTCTTGGCTTGGACAACTATGCACAGGCTTCTGCCATGTACTTGCTCAAGTACAGGCAACTCGAAGCTCACGACAACAACGAAGCTAGTCGAACTCAACGACGACGTCGAAATGAAGACGAGTAA

Coding sequence (CDS)

ATGGGAGATGATAAGAAGGAAGAGCTTCAATTGCCGATTGCCAATGTGGGCAGATTAATGAAGAAGATACTTCCTCAAACGGCGAAGATTTCAAAGGCTGCGAAACAGACGATGCAAGAATGTGCGACAGAGTTCATTGGGTTCGTCACAAGTGAAGCTGCAAACAAATGTCACACACAGAATCGTAGGACTCTCAATGGAGACGACATTCATTCGGCTTTTGCTGCTCTTGGCTTGGACAACTATGCACAGGCTTCTGCCATGTACTTGCTCAAGTACAGGCAACTCGAAGCTCACGACAACAACGAAGCTAGTCGAACTCAACGACGACGTCGAAATGAAGACGAGTAA

Protein sequence

MGDDKKEELQLPIANVGRLMKKILPQTAKISKAAKQTMQECATEFIGFVTSEAANKCHTQNRRTLNGDDIHSAFAALGLDNYAQASAMYLLKYRQLEAHDNNEASRTQRRRRNEDE
Homology
[Close]
BLAST of Lag0041495 vs. NCBI nr
Match: KAG6590402.1 (Nuclear transcription factor Y subunit B-4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 149.4 bits (376), Expect = 1.9e-32
Identity = 76/97 (78.35%), Postives = 82/97 (84.54%), Query Frame = 0 

Query: 1  MGDDKKEELQLPIANVGRLMKKILPQTAKISKAAKQTMQECATEFIGFVTSEAANKCHTQ 60
          MG++  +ELQLPIANVGRLMKKILPQ AKISK AK TMQECATEFI FVTSEAA KCH  
Sbjct: 1  MGENNGDELQLPIANVGRLMKKILPQKAKISKEAKATMQECATEFISFVTSEAAEKCHKD 60

Query: 61 NRRTLNGDDIHSAFAALGLDNYAQASAMYLLKYRQLE 98
          NRRTLNGDDI  AF + GLDNYA+ASA YLLKYR +E
Sbjct: 61 NRRTLNGDDICWAFGSFGLDNYAEASAKYLLKYRDVE 97

[Close]
BLAST of Lag0041495 vs. NCBI nr
Match: XP_022961414.1 (nuclear transcription factor Y subunit B-4-like [Cucurbita moschata])

HSP 1 Score: 149.4 bits (376), Expect = 1.9e-32
Identity = 76/97 (78.35%), Postives = 82/97 (84.54%), Query Frame = 0 

Query: 1  MGDDKKEELQLPIANVGRLMKKILPQTAKISKAAKQTMQECATEFIGFVTSEAANKCHTQ 60
          MG++  +ELQLPIANVGRLMKKILPQ AKISK AK TMQECATEFI FVTSEAA KCH  
Sbjct: 1  MGENNGDELQLPIANVGRLMKKILPQKAKISKEAKATMQECATEFISFVTSEAAEKCHKD 60

Query: 61 NRRTLNGDDIHSAFAALGLDNYAQASAMYLLKYRQLE 98
          NRRTLNGDDI  AF + GLDNYA+ASA YLLKYR +E
Sbjct: 61 NRRTLNGDDICWAFGSFGLDNYAEASAKYLLKYRDVE 97

[Close]
BLAST of Lag0041495 vs. NCBI nr
Match: XP_022968852.1 (nuclear transcription factor Y subunit B-4-like [Cucurbita maxima])

HSP 1 Score: 144.8 bits (364), Expect = 4.6e-31
Identity = 81/119 (68.07%), Postives = 89/119 (74.79%), Query Frame = 0 

Query: 1   MGDDKKEELQLPIANVGRLMKKILPQTAKISKAAKQTMQECATEFIGFVTSEAANKCHTQ 60
           MG++  +ELQLPIANVGRLMKKILPQ AKISK AK TMQECATEFI FVTSEAA KCH  
Sbjct: 1   MGENNGDELQLPIANVGRLMKKILPQKAKISKEAKATMQECATEFISFVTSEAAEKCHKD 60

Query: 61  NRRTLNGDDIHSAFAALGLDNYAQASAMYLLKYRQLE---AHDNNEASRTQRRRRNEDE 117
           NRRTLNGDDI  AF + GLDNYA+ASA YLLKYR +E   A      +R Q+    E E
Sbjct: 61  NRRTLNGDDICWAFGSFGLDNYAEASAKYLLKYRDIERINAQFKTNIAREQQEHGQERE 119

[Close]
BLAST of Lag0041495 vs. NCBI nr
Match: XP_022145916.1 (nuclear transcription factor Y subunit B-4-like [Momordica charantia])

HSP 1 Score: 144.8 bits (364), Expect = 4.6e-31
Identity = 78/119 (65.55%), Postives = 92/119 (77.31%), Query Frame = 0 

Query: 1   MGDDKKEELQLPIANVGRLMKKILPQTAKISKAAKQTMQECATEFIGFVTSEAANKCHTQ 60
           MGDD  E+LQLP ANVGRLMKKI+PQT+KISK AKQTMQECATEFI FVTSEAA KC+ +
Sbjct: 1   MGDDNWEKLQLPSANVGRLMKKIIPQTSKISKEAKQTMQECATEFISFVTSEAAEKCNRE 60

Query: 61  NRRTLNGDDIHSAFAALGLDNYAQASAMYLLKYRQ---LEAHDNNEASRTQRRRRNEDE 117
           NRRTLNG+DI+ AF ALGLDNYA A+A YLL YR    ++ H+ +   + Q    N D+
Sbjct: 61  NRRTLNGEDIYWAFGALGLDNYADATAKYLLNYRDAQTMKIHNKHCGFQQQGEHNNHDD 119

[Close]
BLAST of Lag0041495 vs. NCBI nr
Match: XP_023546470.1 (nuclear transcription factor Y subunit B-4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 144.4 bits (363), Expect = 6.0e-31
Identity = 80/120 (66.67%), Postives = 91/120 (75.83%), Query Frame = 0 

Query: 1   MGDDKKEELQLPIANVGRLMKKILPQTAKISKAAKQTMQECATEFIGFVTSEAANKCHTQ 60
           MG++  +ELQLPIANVGRLMKKILPQ AKISK AK TMQECATEFI FVTSEAA KCH  
Sbjct: 1   MGENNGDELQLPIANVGRLMKKILPQKAKISKEAKATMQECATEFISFVTSEAAEKCHKD 60

Query: 61  NRRTLNGDDIHSAFAALGLDNYAQASAMYLLKYRQLE----AHDNNEASRTQRRRRNEDE 117
           NRRTLNGDDI  AF + GLDNYA+ASA YLLKYR +E        N A   QR  +++++
Sbjct: 61  NRRTLNGDDICWAFGSFGLDNYAEASAKYLLKYRDVERINAQFKTNIAPEQQRHDQDQEQ 120

[Close]
BLAST of Lag0041495 vs. ExPASy Swiss-Prot
Match: O04027 (Nuclear transcription factor Y subunit B-4 OS=Arabidopsis thaliana OX=3702 GN=NFYB4 PE=1 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 4.6e-26
Identity = 66/110 (60.00%), Postives = 79/110 (71.82%), Query Frame = 0 

Query: 7   EELQLPIANVGRLMKKILPQTAKISKAAKQTMQECATEFIGFVTSEAANKCHTQNRRTLN 66
           E+  LPIANVGRLMK+ILP  AKISK AKQT+QECATEFI FVT EA+ KCH +NR+T+N
Sbjct: 4   EDRLLPIANVGRLMKQILPSNAKISKEAKQTVQECATEFISFVTCEASEKCHRENRKTVN 63

Query: 67  GDDIHSAFAALGLDNYAQASAMYLLKYRQLEAHDNNEASRTQRRRRNEDE 117
           GDDI  A + LGLDNYA A   +L KYR+ E  +  E ++      NE E
Sbjct: 64  GDDIWWALSTLGLDNYADAVGRHLHKYREAE-RERTEHNKGSNDSGNEKE 112

[Close]
BLAST of Lag0041495 vs. ExPASy Swiss-Prot
Match: Q54WV0 (Nuclear transcription factor Y subunit beta OS=Dictyostelium discoideum OX=44689 GN=nfyB PE=3 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 1.1e-24
Identity = 51/102 (50.00%), Postives = 78/102 (76.47%), Query Frame = 0 

Query: 4   DKKEELQLPIANVGRLMKKILPQTAKISKAAKQTMQECATEFIGFVTSEAANKCHTQNRR 63
           D++++  LPIAN+ R+MKK LP  AK++K AK+T+Q+C +EFI F+TSEA++KC  + R+
Sbjct: 46  DREQDRYLPIANIIRIMKKALPNNAKVAKDAKETVQDCVSEFISFITSEASDKCQQEKRK 105

Query: 64  TLNGDDIHSAFAALGLDNYAQASAMYLLKYRQLEAHDNNEAS 106
           T+NG+DI +A  +LG +NY +   +YLLKYR+ E + NN+ S
Sbjct: 106 TINGEDIIAAMVSLGFENYVEPLKVYLLKYRETEKNSNNKRS 147

[Close]
BLAST of Lag0041495 vs. ExPASy Swiss-Prot
Match: O82248 (Nuclear transcription factor Y subunit B-5 OS=Arabidopsis thaliana OX=3702 GN=NFYB5 PE=1 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 1.9e-24
Identity = 57/98 (58.16%), Postives = 71/98 (72.45%), Query Frame = 0 

Query: 5   KKEELQLPIANVGRLMKKILPQTAKISKAAKQTMQECATEFIGFVTSEAANKCHTQNRRT 64
           K+++  LPIANVGR+MK ILP  AK+SK AK+TMQEC +EFI FVT EA++KCH + R+T
Sbjct: 50  KEQDRLLPIANVGRIMKNILPANAKVSKEAKETMQECVSEFISFVTGEASDKCHKEKRKT 109

Query: 65  LNGDDIHSAFAALGLDNYAQASAMYLLKYRQLEAHDNN 103
           +NGDDI  A A LG D+YA     YL +YR LE    N
Sbjct: 110 VNGDDICWAMANLGFDDYAAQLKKYLHRYRVLEGEKPN 147

[Close]
BLAST of Lag0041495 vs. ExPASy Swiss-Prot
Match: Q9SIT9 (Nuclear transcription factor Y subunit B-7 OS=Arabidopsis thaliana OX=3702 GN=NFYB7 PE=1 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.1e-22
Identity = 54/112 (48.21%), Postives = 77/112 (68.75%), Query Frame = 0 

Query: 3   DDKKEELQLPIANVGRLMKKILPQTAKISKAAKQTMQECATEFIGFVTSEAANKCHTQNR 62
           ++K+++  LPIANVGR+MKK+LP   KISK AK+T+QEC +EFI FVT EA++KC  + R
Sbjct: 33  NNKEQDRFLPIANVGRIMKKVLPGNGKISKDAKETVQECVSEFISFVTGEASDKCQREKR 92

Query: 63  RTLNGDDIHSAFAALGLDNYAQASAMYLLKYRQLEAHDNNEASRTQRRRRNE 115
           +T+NGDDI  A   LG ++Y     +YL KYR  E    N   + Q+R++ +
Sbjct: 93  KTINGDDIIWAITTLGFEDYVAPLKVYLCKYRDTEGEKVNSPKQQQQRQQQQ 144

[Close]
BLAST of Lag0041495 vs. ExPASy Swiss-Prot
Match: O23310 (Nuclear transcription factor Y subunit B-3 OS=Arabidopsis thaliana OX=3702 GN=NFYB3 PE=1 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 6.9e-22
Identity = 50/102 (49.02%), Postives = 71/102 (69.61%), Query Frame = 0 

Query: 5   KKEELQLPIANVGRLMKKILPQTAKISKAAKQTMQECATEFIGFVTSEAANKCHTQNRRT 64
           ++++  LPIANV R+MKK LP  AKISK AK+T+QEC +EFI F+T EA++KC  + R+T
Sbjct: 20  REQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVSEFISFITGEASDKCQREKRKT 79

Query: 65  LNGDDIHSAFAALGLDNYAQASAMYLLKYRQLEAHDNNEASR 107
           +NGDD+  A   LG ++Y +   +YL KYR++E      A R
Sbjct: 80  INGDDLLWAMTTLGFEDYVEPLKVYLQKYREVEGEKTTTAGR 121

The following BLAST results are available for this feature:
BLAST of Lag0041495 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG6590402.11.9e-3278.35Nuclear transcription factor Y subunit B-4, partial [Cucurbita argyrosperma subs... [more]
XP_022961414.11.9e-3278.35nuclear transcription factor Y subunit B-4-like [Cucurbita moschata][more]
XP_022968852.14.6e-3168.07nuclear transcription factor Y subunit B-4-like [Cucurbita maxima][more]
XP_022145916.14.6e-3165.55nuclear transcription factor Y subunit B-4-like [Momordica charantia][more]
XP_023546470.16.0e-3166.67nuclear transcription factor Y subunit B-4-like [Cucurbita pepo subsp. pepo][more]
BLAST of Lag0041495 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O040274.6e-2660.00Nuclear transcription factor Y subunit B-4 OS=Arabidopsis thaliana OX=3702 GN=NF... [more]
Q54WV01.1e-2450.00Nuclear transcription factor Y subunit beta OS=Dictyostelium discoideum OX=44689... [more]
O822481.9e-2458.16Nuclear transcription factor Y subunit B-5 OS=Arabidopsis thaliana OX=3702 GN=NF... [more]
Q9SIT91.1e-2248.21Nuclear transcription factor Y subunit B-7 OS=Arabidopsis thaliana OX=3702 GN=NF... [more]
O233106.9e-2249.02Nuclear transcription factor Y subunit B-3 OS=Arabidopsis thaliana OX=3702 GN=NF... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00615CCAATSUBUNTAcoord: 76..94
score: 27.63
coord: 38..56
score: 44.14
coord: 57..75
score: 25.24
NoneNo IPR availablePANTHERPTHR11064:SF83NUCLEAR TRANSCRIPTION FACTOR Y SUBUNIT B-4coord: 4..113
IPR003958Transcription factor CBF/NF-Y/archaeal histone domainPFAMPF00808CBFD_NFYB_HMFcoord: 10..73
e-value: 9.8E-23
score: 80.2
IPR009072Histone-foldGENE3D1.10.20.10Histone, subunit Acoord: 2..116
e-value: 1.0E-34
score: 121.3
IPR009072Histone-foldSUPERFAMILY47113Histone-foldcoord: 7..116
IPR027113Transcription factor NFYB/HAP3PANTHERPTHR11064CCAAT-BINDING TRANSCRIPTION FACTOR-RELATEDcoord: 4..113

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0041495.1Lag0041495.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045944 positive regulation of transcription by RNA polymerase II
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0016602 CCAAT-binding factor complex
molecular_function GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific
molecular_function GO:0046982 protein heterodimerization activity
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding