Lag0041375 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0041375
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Rieske domain-containing protein
Location	chr13: 16842993 .. 16844768 (+)
RNA-Seq Expression	Lag0041375
Synteny	Lag0041375

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTCTACAACAGCTCACATTCAGTCAATTTTCAGAAGAAGAAATTAGGGTTTCTTCGATGCCCAAGTTCTTCAGGAACAAGTACGTTTTCCCTTTTCTTCTTCTTCTAATACACCAGATTTATTTCCAATCAAAACCCTATTAGTATATTAATGTGTTCTTTTGGTTATGCTGATGTTAGGCCTTAATCGTTTCTACAAATAAAATGGGACTTTGGTTACGTTGTCATGTATACATCTCTGCTTGGAAGTTCTTCTTGGACCGCAAACTTTTTTAATGTTATGAATATGGCCTTAATCGTTTTCGCCGCTCCCTTTTCACATCTCCTCCGCCCGCTGCCCGAAAAATCTCCTGCAAAGCGTTTGAGATCTCTGTGGCCGAGGAATCATCGACATCTGGTAACTGGGTGCTGGTGGTTCCGTTGTCGGCGTTGCCGAGAGGGGAGCGACGCGTGATTATTCAGCGAGGTGAGACGATTTTGCTTCTTTGGTATAAGGATAAGATTTTTGCTCTTGAGAATAGGTCTCCTGCTGAAGGTGCTTACACTGAAGGCCTCATCAATGCCAAGCTAACGATGGTTGAGTTCTTCCCTTACCCTTTCTTTTATCTAATTTCTTTTGGTTCTGCAGATTTTTTTTTTCAATCGTTGTACTTGCCAATGCATTGGGCACCTGATTTTGAGAGAACCGTAGAATTCTCAGAATTTCATTGAAAACAAAGAAGTCCTATTTCACTATTTCGTTGCCCCTTCTTTATTTTGGTTCTTGGAGGATGATGCAGACAAATTGCCCAATTGGAGTTGCATTCTTATGAGAAGGAGGTAGAGAGAGAAGCTGCCAAAACAGCTAACATGAAAGAAAAAGGAGCTGACCATTATGACCTTAAGCAACAGGTACATAAATGTTTTGTATTCTTCCTAATCAGAACTTTGAATGATTGTTGTCTGCTGACTGCTTGTGGCTTTGTTTGTGCCATCTTTGAATATCTAAAACATGACTTCTTAGACATTCATAACCTTGTGATAGACTAAAAATATGAACATTAGAATCTGTAAGCGTGAAGATAAGTGATCTAGAATATGAAACGAAGAATGCTCTCAAATTTGGAGTCTAGAGATGGTCATCAGGGTAGAACTTTTGAAATTGTCCATACCCTTTAACTTCCTCATACCGTAGTTGAACTTTATTTTCAAAAAGGTATGAAGTCAAAACAAGCAAATACCAAAACCCGAGAAAACTTAACTGGCCATGAAGTGTCAAAACAAAAAGGAAAAAAAATCACATAGTTTGAATTGACTGTCTCGTTATCATGACAACTTCTAAATTTATCAAGTGTTGGATTGTGGAGGTGTTGATGAATTCATTATCCCCGGTGATTTGATTAGTGTCAATATCTTTCGGCAAAAAGCACGAGTTTTGGTTGTAAGCTTCTACATTTTATTCTTCTCTTTGCCATTTGGTCGTAAGCCTTCAAGCTTCTACCTTTTAATTCTCCGGAGAAAAATAACCCCCCATTTCTGACCATTTGTGAAAACATGCATAAAAACTGATTTAGTTTAATTTTATTCTTCTTGTCTGTTTTGTTTCATATTGAAAAATAAACGGAAATTTCTTTTTTTACCCTTATATTTAGTTAAATGTAATCCAAAAGTGTTTCATTAATAAAATTGAATCTAAAATTCGAAGAAGTGATGGAATTAAGACTCTTCGTATTGTTTGTTTAATAATTTACAGGTAGGTGCAGACATTGTTTTGGCTGCTCAGAGTCACTTGTGA

mRNA sequence

ATGTTCTACAACAGCTCACATTCAGTCAATTTTCAGAAGAAGAAATTAGGGTTTCTTCGATGCCCAAGTTCTTCAGGAACAACGTTTGAGATCTCTGTGGCCGAGGAATCATCGACATCTGGTAACTGGGTGCTGGTGGTTCCGTTGTCGGCGTTGCCGAGAGGGGAGCGACGCGTGATTATTCAGCGAGGTGAGACGATTTTGCTTCTTTGGTATAAGGATAAGATTTTTGCTCTTGAGAATAGGTCTCCTGCTGAAGGTGCTTACACTGAAGGCCTCATCAATGCCAAGCTAACGATGGTTGAACAAATTGCCCAATTGGAGTTGCATTCTTATGAGAAGGAGGTAGAGAGAGAAGCTGCCAAAACAGCTAACATGAAAGAAAAAGGAGCTGACCATTATGACCTTAAGCAACAGGTAGGTGCAGACATTGTTTTGGCTGCTCAGAGTCACTTGTGA

Coding sequence (CDS)

ATGTTCTACAACAGCTCACATTCAGTCAATTTTCAGAAGAAGAAATTAGGGTTTCTTCGATGCCCAAGTTCTTCAGGAACAACGTTTGAGATCTCTGTGGCCGAGGAATCATCGACATCTGGTAACTGGGTGCTGGTGGTTCCGTTGTCGGCGTTGCCGAGAGGGGAGCGACGCGTGATTATTCAGCGAGGTGAGACGATTTTGCTTCTTTGGTATAAGGATAAGATTTTTGCTCTTGAGAATAGGTCTCCTGCTGAAGGTGCTTACACTGAAGGCCTCATCAATGCCAAGCTAACGATGGTTGAACAAATTGCCCAATTGGAGTTGCATTCTTATGAGAAGGAGGTAGAGAGAGAAGCTGCCAAAACAGCTAACATGAAAGAAAAAGGAGCTGACCATTATGACCTTAAGCAACAGGTAGGTGCAGACATTGTTTTGGCTGCTCAGAGTCACTTGTGA

Protein sequence

MFYNSSHSVNFQKKKLGFLRCPSSSGTTFEISVAEESSTSGNWVLVVPLSALPRGERRVIIQRGETILLLWYKDKIFALENRSPAEGAYTEGLINAKLTMVEQIAQLELHSYEKEVEREAAKTANMKEKGADHYDLKQQVGADIVLAAQSHL

Homology

[Close]

BLAST of Lag0041375 vs. NCBI nr
Match: XP_038891275.1 (uncharacterized protein LOC120080616 [Benincasa hispida])

HSP 1 Score: 132.5 bits (332), Expect = 3.1e-27
Identity = 65/70 (92.86%), Postives = 67/70 (95.71%), Query Frame = 0


Query: 30  EISVAEESSTSGNWVLVVPLSALPRGERRVIIQRGETILLLWYKDKIFALENRSPAEGAY 89
           EISVAEESS SGNWV VVPLSALPRGERRVIIQ GETILLLWYKDKIFA+ENRSPAEGAY
Sbjct: 58  EISVAEESSASGNWVPVVPLSALPRGERRVIIQGGETILLLWYKDKIFAIENRSPAEGAY 117

Query: 90  TEGLINAKLT 100
           TEGL+NAKLT
Sbjct: 118 TEGLLNAKLT 127

[Close]

BLAST of Lag0041375 vs. NCBI nr
Match: XP_008459624.1 (PREDICTED: uncharacterized protein LOC103498694 [Cucumis melo] >KAA0039261.1 uncharacterized protein E6C27_scaffold64G00520 [Cucumis melo var. makuwa] >TYK00447.1 uncharacterized protein E5676_scaffold169G00510 [Cucumis melo var. makuwa])

HSP 1 Score: 131.3 bits (329), Expect = 6.9e-27
Identity = 64/70 (91.43%), Postives = 67/70 (95.71%), Query Frame = 0


Query: 30  EISVAEESSTSGNWVLVVPLSALPRGERRVIIQRGETILLLWYKDKIFALENRSPAEGAY 89
           EISVAEESS SGNWV VVPLSALPRGERRVIIQ GETILLLWYKD+IFA+ENRSPAEGAY
Sbjct: 58  EISVAEESSASGNWVPVVPLSALPRGERRVIIQGGETILLLWYKDRIFAIENRSPAEGAY 117

Query: 90  TEGLINAKLT 100
           TEGL+NAKLT
Sbjct: 118 TEGLLNAKLT 127

[Close]

BLAST of Lag0041375 vs. NCBI nr
Match: XP_004141552.1 (uncharacterized protein LOC101206141 [Cucumis sativus] >KGN52681.1 hypothetical protein Csa_008629 [Cucumis sativus])

HSP 1 Score: 131.3 bits (329), Expect = 6.9e-27
Identity = 64/70 (91.43%), Postives = 67/70 (95.71%), Query Frame = 0


Query: 30  EISVAEESSTSGNWVLVVPLSALPRGERRVIIQRGETILLLWYKDKIFALENRSPAEGAY 89
           EISVAEESS SGNWV VVPLSALPRGERRVIIQ GETILLLWYKD+IFA+ENRSPAEGAY
Sbjct: 58  EISVAEESSASGNWVPVVPLSALPRGERRVIIQGGETILLLWYKDRIFAIENRSPAEGAY 117

Query: 90  TEGLINAKLT 100
           TEGL+NAKLT
Sbjct: 118 TEGLLNAKLT 127

[Close]

BLAST of Lag0041375 vs. NCBI nr
Match: XP_022973986.1 (uncharacterized protein LOC111472599 [Cucurbita maxima])

HSP 1 Score: 129.0 bits (323), Expect = 3.4e-26
Identity = 61/70 (87.14%), Postives = 67/70 (95.71%), Query Frame = 0


Query: 30  EISVAEESSTSGNWVLVVPLSALPRGERRVIIQRGETILLLWYKDKIFALENRSPAEGAY 89
           E+SVAEESS SGNWV VVPLSALP+GERRVIIQ GET+LLLWYK+KIFA+ENRSPAEGAY
Sbjct: 58  EVSVAEESSASGNWVPVVPLSALPKGERRVIIQGGETVLLLWYKEKIFAIENRSPAEGAY 117

Query: 90  TEGLINAKLT 100
           TEGL+NAKLT
Sbjct: 118 TEGLLNAKLT 127

[Close]

BLAST of Lag0041375 vs. NCBI nr
Match: KAG7025185.1 (hypothetical protein SDJN02_14008 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 127.9 bits (320), Expect = 7.6e-26
Identity = 60/70 (85.71%), Postives = 67/70 (95.71%), Query Frame = 0


Query: 30  EISVAEESSTSGNWVLVVPLSALPRGERRVIIQRGETILLLWYKDKIFALENRSPAEGAY 89
           E+SVAEESS SGNWV VVPLSALP+GERRVIIQ GET+LLLWYK++IFA+ENRSPAEGAY
Sbjct: 58  EVSVAEESSASGNWVPVVPLSALPKGERRVIIQGGETVLLLWYKERIFAIENRSPAEGAY 117

Query: 90  TEGLINAKLT 100
           TEGL+NAKLT
Sbjct: 118 TEGLLNAKLT 127

[Close]

BLAST of Lag0041375 vs. ExPASy Swiss-Prot
Match: O04350 (Tubulin-folding cofactor A OS=Arabidopsis thaliana OX=3702 GN=TFCA PE=1 SV=2)

HSP 1 Score: 58.9 bits (141), Expect = 5.7e-08
Identity = 29/32 (90.62%), Postives = 31/32 (96.88%), Query Frame = 0


Query: 108 ELHSYEKEVEREAAKTANMKEKGADHYDLKQQ 140
           ELHSYEKEVEREAAKTA+MK+KGAD YDLKQQ
Sbjct: 20  ELHSYEKEVEREAAKTADMKDKGADPYDLKQQ 51

The following BLAST results are available for this feature:

BLAST of Lag0041375 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038891275.1	3.1e-27	92.86	uncharacterized protein LOC120080616 [Benincasa hispida]	[more]
XP_008459624.1	6.9e-27	91.43	PREDICTED: uncharacterized protein LOC103498694 [Cucumis melo] >KAA0039261.1 unc...	[more]
XP_004141552.1	6.9e-27	91.43	uncharacterized protein LOC101206141 [Cucumis sativus] >KGN52681.1 hypothetical ...	[more]
XP_022973986.1	3.4e-26	87.14	uncharacterized protein LOC111472599 [Cucurbita maxima]	[more]
KAG7025185.1	7.6e-26	85.71	hypothetical protein SDJN02_14008 [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Lag0041375 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04350	5.7e-08	90.63	Tubulin-folding cofactor A OS=Arabidopsis thaliana OX=3702 GN=TFCA PE=1 SV=2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 109..129
None	No IPR available	GENE3D	1.20.58.90		coord: 94..147 e-value: 1.9E-8 score: 36.4
None	No IPR available	PANTHER	PTHR43456:SF2	RIESKE (2FE-2S) DOMAIN-CONTAINING PROTEIN	coord: 12..100
None	No IPR available	PANTHER	PTHR43456	RIESKE (2FE-2S) DOMAIN-CONTAINING PROTEIN	coord: 12..100
IPR012748	Rieske-like [2Fe-2S] domain, NirD-type	PFAM	PF13806	Rieske_2	coord: 42..97 e-value: 1.5E-7 score: 31.3
IPR036126	Tubulin binding cofactor A superfamily	SUPERFAMILY	46988	Tubulin chaperone cofactor A	coord: 108..140
IPR036922	Rieske [2Fe-2S] iron-sulphur domain superfamily	SUPERFAMILY	50022	ISP domain	coord: 42..94

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0041375.1	Lag0041375.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042128	nitrate assimilation
biological_process	GO:0007023	post-chaperonin tubulin folding pathway
biological_process	GO:0007021	tubulin complex assembly
cellular_component	GO:0031967	organelle envelope
molecular_function	GO:0051537	2 iron, 2 sulfur cluster binding
molecular_function	GO:0048487	beta-tubulin binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0008942	nitrite reductase [NAD(P)H] activity