Lag0041328 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0041328 vs. NCBI nr
Match: XP_023527413.1 (ADP-ribosylation factor GTPase-activating protein AGD4-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 644.8 bits (1662), Expect = 4.4e-181
Identity = 324/366 (88.52%), Postives = 343/366 (93.72%), Query Frame = 0

Query: 1   MDWVNKITGAIASLFNSYFLQQPFLEAKHPESDKNNFDNYNVQTRHQERL-DGSQVDSVS 60
           MDWVNKI GAI SLFNSYFLQQPFLEAKHPE+DKN+FDNYNVQT+HQ+R+ DGSQ DSVS
Sbjct: 411 MDWVNKIMGAITSLFNSYFLQQPFLEAKHPENDKNHFDNYNVQTQHQKRIEDGSQGDSVS 470

Query: 61  KILREIPGNDLCAECGSPEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWE 120
           KILREIPGND+CAECG+PEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWE
Sbjct: 471 KILREIPGNDICAECGTPEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWE 530

Query: 121 PSILDLFRNLGNAYCNSIWEGLLLADIGSDDTQ------ISKPSPKDAIQHRERYIQAKY 180
           PSILDLFRNLGNAYCNSIWEGLLLADIGSD+TQ      I KPSPKDAIQ RE+YIQAKY
Sbjct: 531 PSILDLFRNLGNAYCNSIWEGLLLADIGSDNTQHAVVTSIPKPSPKDAIQRREKYIQAKY 590

Query: 181 VEKLLVVKDSEEPGNISYAKGIWEAVKTNDLREVYHLIAVSAVSIVNTTFDHVVSVSSSP 240
           VEKLL+VKD+E  GN SYAK IWEAV+TNDLREVY LIAVSAVSIVN T+DH VSVSSSP
Sbjct: 591 VEKLLLVKDTEASGNNSYAKSIWEAVQTNDLREVYRLIAVSAVSIVNKTYDHAVSVSSSP 650

Query: 241 HLNEEPSGSNRESHSPSLCGRDSDSNESRNNLQGCSLLHLACQNDNQVMLELLLQFGADI 300
           +L+EEPSG NRESH+PS CGRD DSNESR++LQGCSLLHLACQNDNQVMLELLLQFGADI
Sbjct: 651 NLDEEPSGGNRESHNPSSCGRDMDSNESRSSLQGCSLLHLACQNDNQVMLELLLQFGADI 710

Query: 301 NVRDSHGRTPLHQCISQKNNTLAKLLLRRGARPSIKDGGGLSALERAMEMGAITDDELFL 360
           NVRDSHGRTPLHQCISQKNNTLAKLLLRRGAR SIKDGGGLSALERAMEMG+ITDDELFL
Sbjct: 711 NVRDSHGRTPLHQCISQKNNTLAKLLLRRGARQSIKDGGGLSALERAMEMGSITDDELFL 770

BLAST of Lag0041328 vs. NCBI nr
Match: XP_023527405.1 (ADP-ribosylation factor GTPase-activating protein AGD4-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 644.8 bits (1662), Expect = 4.4e-181
Identity = 324/366 (88.52%), Postives = 343/366 (93.72%), Query Frame = 0

Query: 1   MDWVNKITGAIASLFNSYFLQQPFLEAKHPESDKNNFDNYNVQTRHQERL-DGSQVDSVS 60
           MDWVNKI GAI SLFNSYFLQQPFLEAKHPE+DKN+FDNYNVQT+HQ+R+ DGSQ DSVS
Sbjct: 412 MDWVNKIMGAITSLFNSYFLQQPFLEAKHPENDKNHFDNYNVQTQHQKRIEDGSQGDSVS 471

Query: 61  KILREIPGNDLCAECGSPEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWE 120
           KILREIPGND+CAECG+PEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWE
Sbjct: 472 KILREIPGNDICAECGTPEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWE 531

Query: 121 PSILDLFRNLGNAYCNSIWEGLLLADIGSDDTQ------ISKPSPKDAIQHRERYIQAKY 180
           PSILDLFRNLGNAYCNSIWEGLLLADIGSD+TQ      I KPSPKDAIQ RE+YIQAKY
Sbjct: 532 PSILDLFRNLGNAYCNSIWEGLLLADIGSDNTQHAVVTSIPKPSPKDAIQRREKYIQAKY 591

Query: 181 VEKLLVVKDSEEPGNISYAKGIWEAVKTNDLREVYHLIAVSAVSIVNTTFDHVVSVSSSP 240
           VEKLL+VKD+E  GN SYAK IWEAV+TNDLREVY LIAVSAVSIVN T+DH VSVSSSP
Sbjct: 592 VEKLLLVKDTEASGNNSYAKSIWEAVQTNDLREVYRLIAVSAVSIVNKTYDHAVSVSSSP 651

Query: 241 HLNEEPSGSNRESHSPSLCGRDSDSNESRNNLQGCSLLHLACQNDNQVMLELLLQFGADI 300
           +L+EEPSG NRESH+PS CGRD DSNESR++LQGCSLLHLACQNDNQVMLELLLQFGADI
Sbjct: 652 NLDEEPSGGNRESHNPSSCGRDMDSNESRSSLQGCSLLHLACQNDNQVMLELLLQFGADI 711

Query: 301 NVRDSHGRTPLHQCISQKNNTLAKLLLRRGARPSIKDGGGLSALERAMEMGAITDDELFL 360
           NVRDSHGRTPLHQCISQKNNTLAKLLLRRGAR SIKDGGGLSALERAMEMG+ITDDELFL
Sbjct: 712 NVRDSHGRTPLHQCISQKNNTLAKLLLRRGARQSIKDGGGLSALERAMEMGSITDDELFL 771

BLAST of Lag0041328 vs. NCBI nr
Match: KAG7030562.1 (ADP-ribosylation factor GTPase-activating protein AGD4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 644.0 bits (1660), Expect = 7.4e-181
Identity = 323/366 (88.25%), Postives = 342/366 (93.44%), Query Frame = 0

Query: 1   MDWVNKITGAIASLFNSYFLQQPFLEAKHPESDKNNFDNYNVQTRHQERL-DGSQVDSVS 60
           MDWVNKI GAI SLFNSYFLQQPFLEAKHPE+DKN+FDNYNVQT+HQ+R+ DGSQ DSVS
Sbjct: 416 MDWVNKIMGAITSLFNSYFLQQPFLEAKHPENDKNHFDNYNVQTQHQKRIEDGSQGDSVS 475

Query: 61  KILREIPGNDLCAECGSPEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWE 120
           KILREIPGND+CAECG+PEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWE
Sbjct: 476 KILREIPGNDICAECGTPEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWE 535

Query: 121 PSILDLFRNLGNAYCNSIWEGLLLADIGSDDTQ------ISKPSPKDAIQHRERYIQAKY 180
           PSILDLFRNLGNAYCNSIWEGLLLADIGSD+TQ      I KPSPKDAIQ RE+YI AKY
Sbjct: 536 PSILDLFRNLGNAYCNSIWEGLLLADIGSDNTQHAVVTSIPKPSPKDAIQRREKYIHAKY 595

Query: 181 VEKLLVVKDSEEPGNISYAKGIWEAVKTNDLREVYHLIAVSAVSIVNTTFDHVVSVSSSP 240
           VEKLL+VKD+E  GN SYAK IWEAV+TNDLREVY LIAVSAVSIVN T+DH VSVSSSP
Sbjct: 596 VEKLLLVKDTEASGNNSYAKSIWEAVQTNDLREVYRLIAVSAVSIVNKTYDHAVSVSSSP 655

Query: 241 HLNEEPSGSNRESHSPSLCGRDSDSNESRNNLQGCSLLHLACQNDNQVMLELLLQFGADI 300
           HL+EEPSG NRESH+PS CGRD DSNESR++LQGCSLLHLACQNDNQVMLELLLQFGADI
Sbjct: 656 HLDEEPSGGNRESHNPSSCGRDMDSNESRSSLQGCSLLHLACQNDNQVMLELLLQFGADI 715

Query: 301 NVRDSHGRTPLHQCISQKNNTLAKLLLRRGARPSIKDGGGLSALERAMEMGAITDDELFL 360
           NVRDSHGRTPLHQCIS+KNNTLAKLLLRRGAR SIKDGGGLSALERAMEMG+ITDDELFL
Sbjct: 716 NVRDSHGRTPLHQCISRKNNTLAKLLLRRGARQSIKDGGGLSALERAMEMGSITDDELFL 775

BLAST of Lag0041328 vs. NCBI nr
Match: XP_022942355.1 (ADP-ribosylation factor GTPase-activating protein AGD4-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 644.0 bits (1660), Expect = 7.4e-181
Identity = 323/366 (88.25%), Postives = 342/366 (93.44%), Query Frame = 0

Query: 1   MDWVNKITGAIASLFNSYFLQQPFLEAKHPESDKNNFDNYNVQTRHQERL-DGSQVDSVS 60
           MDWVNKI GAI SLFNSYFLQQPFLEAKHPE+DKN+FDNYNVQT+HQ+R+ DGSQ DSVS
Sbjct: 416 MDWVNKIMGAITSLFNSYFLQQPFLEAKHPENDKNHFDNYNVQTQHQKRIEDGSQGDSVS 475

Query: 61  KILREIPGNDLCAECGSPEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWE 120
           KILREIPGND+CAECG+PEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWE
Sbjct: 476 KILREIPGNDICAECGTPEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWE 535

Query: 121 PSILDLFRNLGNAYCNSIWEGLLLADIGSDDTQ------ISKPSPKDAIQHRERYIQAKY 180
           PSILDLFRNLGNAYCNSIWEGLLLADIGSD+TQ      I KPSPKDAIQ RE+YI AKY
Sbjct: 536 PSILDLFRNLGNAYCNSIWEGLLLADIGSDNTQHAVVTSIPKPSPKDAIQRREKYIHAKY 595

Query: 181 VEKLLVVKDSEEPGNISYAKGIWEAVKTNDLREVYHLIAVSAVSIVNTTFDHVVSVSSSP 240
           VEKLL+VKD+E  GN SYAK IWEAV+TNDLREVY LIAVSAVSIVN T+DH VSVSSSP
Sbjct: 596 VEKLLLVKDTEASGNNSYAKSIWEAVQTNDLREVYRLIAVSAVSIVNKTYDHAVSVSSSP 655

Query: 241 HLNEEPSGSNRESHSPSLCGRDSDSNESRNNLQGCSLLHLACQNDNQVMLELLLQFGADI 300
           HL+EEPSG NRESH+PS CGR+ DSNESR++LQGCSLLHLACQNDNQVMLELLLQFGADI
Sbjct: 656 HLDEEPSGGNRESHNPSSCGREMDSNESRSSLQGCSLLHLACQNDNQVMLELLLQFGADI 715

Query: 301 NVRDSHGRTPLHQCISQKNNTLAKLLLRRGARPSIKDGGGLSALERAMEMGAITDDELFL 360
           NVRDSHGRTPLHQCISQKNNTLAKLLLRRGAR SIKDGGGLSALERAMEMG+ITDDELFL
Sbjct: 716 NVRDSHGRTPLHQCISQKNNTLAKLLLRRGARQSIKDGGGLSALERAMEMGSITDDELFL 775

BLAST of Lag0041328 vs. NCBI nr
Match: XP_022942353.1 (ADP-ribosylation factor GTPase-activating protein AGD4-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 644.0 bits (1660), Expect = 7.4e-181
Identity = 323/366 (88.25%), Postives = 342/366 (93.44%), Query Frame = 0

Query: 1   MDWVNKITGAIASLFNSYFLQQPFLEAKHPESDKNNFDNYNVQTRHQERL-DGSQVDSVS 60
           MDWVNKI GAI SLFNSYFLQQPFLEAKHPE+DKN+FDNYNVQT+HQ+R+ DGSQ DSVS
Sbjct: 417 MDWVNKIMGAITSLFNSYFLQQPFLEAKHPENDKNHFDNYNVQTQHQKRIEDGSQGDSVS 476

Query: 61  KILREIPGNDLCAECGSPEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWE 120
           KILREIPGND+CAECG+PEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWE
Sbjct: 477 KILREIPGNDICAECGTPEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWE 536

Query: 121 PSILDLFRNLGNAYCNSIWEGLLLADIGSDDTQ------ISKPSPKDAIQHRERYIQAKY 180
           PSILDLFRNLGNAYCNSIWEGLLLADIGSD+TQ      I KPSPKDAIQ RE+YI AKY
Sbjct: 537 PSILDLFRNLGNAYCNSIWEGLLLADIGSDNTQHAVVTSIPKPSPKDAIQRREKYIHAKY 596

Query: 181 VEKLLVVKDSEEPGNISYAKGIWEAVKTNDLREVYHLIAVSAVSIVNTTFDHVVSVSSSP 240
           VEKLL+VKD+E  GN SYAK IWEAV+TNDLREVY LIAVSAVSIVN T+DH VSVSSSP
Sbjct: 597 VEKLLLVKDTEASGNNSYAKSIWEAVQTNDLREVYRLIAVSAVSIVNKTYDHAVSVSSSP 656

Query: 241 HLNEEPSGSNRESHSPSLCGRDSDSNESRNNLQGCSLLHLACQNDNQVMLELLLQFGADI 300
           HL+EEPSG NRESH+PS CGR+ DSNESR++LQGCSLLHLACQNDNQVMLELLLQFGADI
Sbjct: 657 HLDEEPSGGNRESHNPSSCGREMDSNESRSSLQGCSLLHLACQNDNQVMLELLLQFGADI 716

Query: 301 NVRDSHGRTPLHQCISQKNNTLAKLLLRRGARPSIKDGGGLSALERAMEMGAITDDELFL 360
           NVRDSHGRTPLHQCISQKNNTLAKLLLRRGAR SIKDGGGLSALERAMEMG+ITDDELFL
Sbjct: 717 NVRDSHGRTPLHQCISQKNNTLAKLLLRRGARQSIKDGGGLSALERAMEMGSITDDELFL 776

BLAST of Lag0041328 vs. ExPASy Swiss-Prot
Match: Q9SMX5 (ADP-ribosylation factor GTPase-activating protein AGD4 OS=Arabidopsis thaliana OX=3702 GN=AGD4 PE=2 SV=2)

HSP 1 Score: 406.0 bits (1042), Expect = 4.5e-112
Identity = 211/365 (57.81%), Postives = 264/365 (72.33%), Query Frame = 0

Query: 1   MDWVNKITGAIASLFNSYFLQQP---FLEAKHPESDKNNFDNYNVQTRHQERLDGSQVDS 60
           MDWVNKIT AI +L NS+FLQQ    +L+  +  S   N      Q RH +       D 
Sbjct: 410 MDWVNKITKAIGTLLNSHFLQQSPVRYLDKDNSSSAPANAVVSGDQIRHNDSRQNIG-DD 469

Query: 61  VSKILREIPGNDLCAECGSPEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKV 120
           VS ILR +PGN+ CAEC +PEP+WASLNLG+LLCI+CSGVHRNLGVHISKVRS+ LDVKV
Sbjct: 470 VSTILRGLPGNNACAECNAPEPDWASLNLGVLLCIQCSGVHRNLGVHISKVRSLSLDVKV 529

Query: 121 WEPSILDLFRNLGNAYCNSIWEGLLLADIGSDD------TQISKPSPKDAIQHRERYIQA 180
           WEP+ILDLFRNLGN YCNS+WEGLL  D   +D        +SKP P+D+   +E+YI  
Sbjct: 530 WEPTILDLFRNLGNVYCNSLWEGLLHLDDDCEDGSALSHASVSKPCPEDSFSVKEKYILG 589

Query: 181 KYVEKLLVVKDSEEPGNISYAKGIWEAVKTNDLREVYHLIAVSA-VSIVNTTFDHVVSVS 240
           KY+EK LV+KD  E  N+S A  IWEAV++ ++RE+Y LI  +  V+I+NT FD +  + 
Sbjct: 590 KYLEKALVIKDESE-ANLSAASRIWEAVQSRNIREIYRLIVTTGDVNIINTKFDDITDID 649

Query: 241 SSPHLNEEPSGSNRESHSPSLCGRDSDSNESRNNLQGCSLLHLACQNDNQVMLELLLQFG 300
           +  H+ +    + ++ H P++C R  +SNE R+ LQGCSLLH+AC   + V+LELLLQFG
Sbjct: 650 AYHHI-DAAEKAVKKRHDPTVCQRIKESNEPRSCLQGCSLLHVACHIGDSVLLELLLQFG 709

Query: 301 ADINVRDSHGRTPLHQCISQKNNTLAKLLLRRGARPSIKDGGGLSALERAMEMGAITDDE 356
           AD+N+RD HGRTPLH CIS  N+  AK+LLRRGARPSI+D GGLS LERAMEMGAITD+E
Sbjct: 710 ADLNIRDYHGRTPLHHCISSGNHKFAKILLRRGARPSIEDDGGLSVLERAMEMGAITDEE 769

BLAST of Lag0041328 vs. ExPASy Swiss-Prot
Match: Q9C6C3 (ADP-ribosylation factor GTPase-activating protein AGD2 OS=Arabidopsis thaliana OX=3702 GN=AGD2 PE=2 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 2.2e-111
Identity = 211/366 (57.65%), Postives = 266/366 (72.68%), Query Frame = 0

Query: 1   MDWVNKITGAIASLFNSYFLQQP---FLEAKHPESDKNNFD-NYNVQTRHQERLDGSQVD 60
           MDWVNKIT AI    NS+FLQQ    +L+ K+  S     +   N +  + +RL+    D
Sbjct: 410 MDWVNKITAAITIRLNSHFLQQSPARYLDKKNTSSGPATENLTLNQKEDYNQRLNVG--D 469

Query: 61  SVSKILREIPGNDLCAECGSPEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVK 120
            V  ILREIPGN+ CAEC +P+P+WASLNLG+L+CIECSGVHRNLGVHISKVRS+ LDVK
Sbjct: 470 DVLTILREIPGNNTCAECNAPDPDWASLNLGVLMCIECSGVHRNLGVHISKVRSLTLDVK 529

Query: 121 VWEPSILDLFRNLGNAYCNSIWEGLLL-----ADIGSDDT--QISKPSPKDAIQHRERYI 180
           VWEP+ILDLFRNLGN YCNS+WE LL      ++ GS DT   +SKPS +D    +E+YI
Sbjct: 530 VWEPTILDLFRNLGNGYCNSVWEELLHHLDDDSEKGSTDTLASVSKPSSEDWFTLKEKYI 589

Query: 181 QAKYVEKLLVVKDSEEPGNISYAKGIWEAVKTNDLREVYHLIAVSAVSIVNTTFDHVVSV 240
             KY+EK LVVKD  E  N + +  IWEAV++ ++R++Y LI  +  +I+NT FD +  +
Sbjct: 590 NGKYLEKALVVKDERE-ANSTASSRIWEAVQSRNIRDIYRLIVKADANIINTKFDDITDL 649

Query: 241 SSSPHLNEEPSGSNRESHSPSLCGRDSDSNESRNNLQGCSLLHLACQNDNQVMLELLLQF 300
               H + +     ++ H P+ C R  +SNE+RN LQGCSLLH+ACQ+ + ++LELLLQF
Sbjct: 650 DVYHHHHVDAPDEVKKRHDPNACQRIKNSNEARNCLQGCSLLHVACQSGDPILLELLLQF 709

Query: 301 GADINVRDSHGRTPLHQCISQKNNTLAKLLLRRGARPSIKDGGGLSALERAMEMGAITDD 356
           GADIN+RD HGRTPLH CI+  NN  AK+LLRRGARPSI+DGGGLS LERAMEMGAITD+
Sbjct: 710 GADINMRDYHGRTPLHHCIASGNNAFAKVLLRRGARPSIEDGGGLSVLERAMEMGAITDE 769

BLAST of Lag0041328 vs. ExPASy Swiss-Prot
Match: Q5W7F2 (ADP-ribosylation factor GTPase-activating protein AGD3 OS=Arabidopsis thaliana OX=3702 GN=AGD3 PE=1 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 9.8e-67
Identity = 169/405 (41.73%), Postives = 227/405 (56.05%), Query Frame = 0

Query: 1   MDWVNKITGAIASLFNSYFLQQPF---------------------LEAKHPESD----KN 60
           MDW+ KITG IASL +S   +Q                        E  HP ++    + 
Sbjct: 419 MDWIEKITGVIASLLSSQVPEQRLPGSPMGSGHHRSASESSSYESSEYDHPTTEEFVCER 478

Query: 61  NFDNYN------VQTRHQERLDGSQVDSVSKILREIPGNDLCAECGSPEPEWASLNLGIL 120
           +F  YN       Q +   R     +D+    LR++ GND CA+CG+PEP+WASLNLG+L
Sbjct: 479 SFLGYNERPSRSFQPQRSIRKGEKPIDA----LRKVCGNDKCADCGAPEPDWASLNLGVL 538

Query: 121 LCIECSGVHRNLGVHISKVRSIILDVKVWEPSILDLFRNLGNAYCNSIWEGLLLA----- 180
           +CIECSGVHRNLGVHISKVRS+ LDVKVWEPS++ LF+ LGN + N++WE LL +     
Sbjct: 539 VCIECSGVHRNLGVHISKVRSLTLDVKVWEPSVISLFQALGNTFANTVWEELLHSRSAIH 598

Query: 181 -DIG---SDDTQI---SKPSPKDAIQHRERYIQAKYVEKLLVVKDSEEPGNISYAKGIWE 240
            D G   SD +++    KPS  D I  +E+YIQAKY EKL V +  +     S A+ +W+
Sbjct: 599 FDPGLTVSDKSRVMVTGKPSYADMISIKEKYIQAKYAEKLFVRRSRDSDFPQSAAQQMWD 658

Query: 241 AVKTNDLREVYHLIAVSAVSIVNTTFDHVVSVS-SSPHLNEEPSGSNRES-----HSPSL 300
           AV  ND + VY LI V+  + VN  +D   S S +   +   P    RE       +  L
Sbjct: 659 AVSGNDKKAVYRLI-VNGDADVNYVYDQTSSSSLTLSRVILVPERPKREDVLLRLRNELL 718

Query: 301 CGRDSDSNESRNNLQGCSLLHLACQNDNQVMLELLLQFGADINVRDSHGRTPLHQCISQK 357
               S SN S     G SLLH AC+  +  M+ELLLQ+GA++N  DS G+TPLH C+ + 
Sbjct: 719 DRTGSSSNISPEGSGGSSLLHCACEKADLGMVELLLQYGANVNASDSSGQTPLHCCLLRG 778

BLAST of Lag0041328 vs. ExPASy Swiss-Prot
Match: Q9FIT8 (ADP-ribosylation factor GTPase-activating protein AGD1 OS=Arabidopsis thaliana OX=3702 GN=AGD1 PE=2 SV=2)

HSP 1 Score: 236.9 bits (603), Expect = 3.6e-61
Identity = 158/417 (37.89%), Postives = 220/417 (52.76%), Query Frame = 0

Query: 1   MDWVNKITGAIASLFNSYFLQQPFLEAKHPE-------SDKNNF-DNYNVQTR------- 60
           MDW+ KITG IASL +    ++  +     +       SD  +  D Y+++         
Sbjct: 414 MDWIEKITGVIASLLSFQTPERAIMRLSTVDGGDTFSASDSGSLADPYDIEQAESGESTV 473

Query: 61  -------HQERLDG--SQVDSVS-----KILREIPGNDLCAECGSPEPEWASLNLGILLC 120
                  ++ R  G   Q D V       +L  + GN+ CA+CG+PEP+WASLNLG+L+C
Sbjct: 474 EHPMTGGNRSRFSGCLQQHDMVKTEKPIDVLTRVLGNERCADCGAPEPDWASLNLGVLIC 533

Query: 121 IECSGVHRNLGVHISKVRSIILDVKVWEPSILDLFRNLGNAYCNSIWEGLLLADIGSDDT 180
           IECSG+HRNLGVHISKVRS+ LDVKVWEPS+L LF++LGN Y NS+WE LL ++  +   
Sbjct: 534 IECSGIHRNLGVHISKVRSLTLDVKVWEPSVLTLFQSLGNVYVNSVWEELLNSESRTSSA 593

Query: 181 QISKPSPK---------------DAIQHRERYIQAKYVEKLLVVKDSEEPGNISYAKGIW 240
             S  +PK               D I  +E +I AKY E++ V K  +     +  + IW
Sbjct: 594 SRSSGTPKSDRPRKLLVRKPGFNDPISVKELFIHAKYSERIFVRKAIDSQHFQAVFQEIW 653

Query: 241 EAVKTNDLREVY-HLIAVSA-VSIVNTTFDHVVSVSSSPHLN------------EEPSGS 300
           E V+ ND + VY H++   A V+ +     + VS+  S  +             EE    
Sbjct: 654 ENVRANDKKSVYRHIVCSEADVNALRGQASYTVSLPLSKMMQMEETLEAKFKSIEEEFQE 713

Query: 301 NRESHSPSLCGRDSDSNESRNNLQGCSLLHLACQNDNQVMLELLLQFGADINVRDSHGRT 360
           N   +S S    +S   E  +N   CSLLHLAC + +  M+ELLLQ+GA IN  DS GRT
Sbjct: 714 NPAGYSNSRGDGESMVREETSN--DCSLLHLACLSADIGMVELLLQYGAKINATDSKGRT 773

BLAST of Lag0041328 vs. ExPASy Swiss-Prot
Match: Q8VHH5 (Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 3 OS=Mus musculus OX=10090 GN=Agap3 PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 1.1e-33
Identity = 90/279 (32.26%), Postives = 135/279 (48.39%), Query Frame = 0

Query: 45  RHQERLDGSQVDSVSKILREIPGNDLCAECGSPEPEWASLNLGILLCIECSGVHRNLGVH 104
           + + RL         + +R + GN LC +C +P P+WASLNLG L+CIECSG+HR+LG H
Sbjct: 650 KDKTRLGNQNTALAVQAVRTVRGNSLCIDCEAPNPDWASLNLGALMCIECSGIHRHLGAH 709

Query: 105 ISKVRSIILDVKVWEPSILDLFRNLGNAYCNSIWEGLLLADIGSDDTQISKPSPKDAIQH 164
           +S+VRS  LD+  W P +L +   +GNA  NS+WEG L           SKP P+   + 
Sbjct: 710 LSRVRS--LDLDDWPPELLAVMTAMGNALANSVWEGAL--------DGYSKPGPEACREE 769

Query: 165 RERYIQAKYVEKLLVVKDSEEPGNISYAKGIWEAVKTNDLREVYHLIAVSAVSIVNTTFD 224
           +ER+I+AKY +KL +        ++   + +  AV  +DLR +  L+A  +   VN T+ 
Sbjct: 770 KERWIRAKYEQKLFLA--PLPSSDVPLGQQLLRAVVEDDLRLLVMLLAHGSKEEVNETYG 829

Query: 225 HVVSVSSSPHLNEEPSGSNRESHSPSLCGRDSDSNESRNNLQGCSLLHLACQNDNQVMLE 284
                                         D D         G + LHL+    N V  +
Sbjct: 830 ------------------------------DGD---------GRTALHLSSAMANVVFTQ 877

Query: 285 LLLQFGADINVRDSHGRTPLHQCISQKNNTLAKLLLRRG 324
           LL+ +G D+  RD+ G TPL       +   A +L++ G
Sbjct: 890 LLIWYGVDVRSRDARGLTPLAYARRAGSQECADILIQHG 877

BLAST of Lag0041328 vs. ExPASy TrEMBL
Match: A0A6J1FW51 (ADP-ribosylation factor GTPase-activating protein AGD4-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111447422 PE=4 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 3.6e-181
Identity = 323/366 (88.25%), Postives = 342/366 (93.44%), Query Frame = 0

Query: 1   MDWVNKITGAIASLFNSYFLQQPFLEAKHPESDKNNFDNYNVQTRHQERL-DGSQVDSVS 60
           MDWVNKI GAI SLFNSYFLQQPFLEAKHPE+DKN+FDNYNVQT+HQ+R+ DGSQ DSVS
Sbjct: 416 MDWVNKIMGAITSLFNSYFLQQPFLEAKHPENDKNHFDNYNVQTQHQKRIEDGSQGDSVS 475

Query: 61  KILREIPGNDLCAECGSPEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWE 120
           KILREIPGND+CAECG+PEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWE
Sbjct: 476 KILREIPGNDICAECGTPEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWE 535

Query: 121 PSILDLFRNLGNAYCNSIWEGLLLADIGSDDTQ------ISKPSPKDAIQHRERYIQAKY 180
           PSILDLFRNLGNAYCNSIWEGLLLADIGSD+TQ      I KPSPKDAIQ RE+YI AKY
Sbjct: 536 PSILDLFRNLGNAYCNSIWEGLLLADIGSDNTQHAVVTSIPKPSPKDAIQRREKYIHAKY 595

Query: 181 VEKLLVVKDSEEPGNISYAKGIWEAVKTNDLREVYHLIAVSAVSIVNTTFDHVVSVSSSP 240
           VEKLL+VKD+E  GN SYAK IWEAV+TNDLREVY LIAVSAVSIVN T+DH VSVSSSP
Sbjct: 596 VEKLLLVKDTEASGNNSYAKSIWEAVQTNDLREVYRLIAVSAVSIVNKTYDHAVSVSSSP 655

Query: 241 HLNEEPSGSNRESHSPSLCGRDSDSNESRNNLQGCSLLHLACQNDNQVMLELLLQFGADI 300
           HL+EEPSG NRESH+PS CGR+ DSNESR++LQGCSLLHLACQNDNQVMLELLLQFGADI
Sbjct: 656 HLDEEPSGGNRESHNPSSCGREMDSNESRSSLQGCSLLHLACQNDNQVMLELLLQFGADI 715

Query: 301 NVRDSHGRTPLHQCISQKNNTLAKLLLRRGARPSIKDGGGLSALERAMEMGAITDDELFL 360
           NVRDSHGRTPLHQCISQKNNTLAKLLLRRGAR SIKDGGGLSALERAMEMG+ITDDELFL
Sbjct: 716 NVRDSHGRTPLHQCISQKNNTLAKLLLRRGARQSIKDGGGLSALERAMEMGSITDDELFL 775

BLAST of Lag0041328 vs. ExPASy TrEMBL
Match: A0A6J1FUL6 (ADP-ribosylation factor GTPase-activating protein AGD4-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447422 PE=4 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 3.6e-181
Identity = 323/366 (88.25%), Postives = 342/366 (93.44%), Query Frame = 0

Query: 1   MDWVNKITGAIASLFNSYFLQQPFLEAKHPESDKNNFDNYNVQTRHQERL-DGSQVDSVS 60
           MDWVNKI GAI SLFNSYFLQQPFLEAKHPE+DKN+FDNYNVQT+HQ+R+ DGSQ DSVS
Sbjct: 417 MDWVNKIMGAITSLFNSYFLQQPFLEAKHPENDKNHFDNYNVQTQHQKRIEDGSQGDSVS 476

Query: 61  KILREIPGNDLCAECGSPEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWE 120
           KILREIPGND+CAECG+PEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWE
Sbjct: 477 KILREIPGNDICAECGTPEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWE 536

Query: 121 PSILDLFRNLGNAYCNSIWEGLLLADIGSDDTQ------ISKPSPKDAIQHRERYIQAKY 180
           PSILDLFRNLGNAYCNSIWEGLLLADIGSD+TQ      I KPSPKDAIQ RE+YI AKY
Sbjct: 537 PSILDLFRNLGNAYCNSIWEGLLLADIGSDNTQHAVVTSIPKPSPKDAIQRREKYIHAKY 596

Query: 181 VEKLLVVKDSEEPGNISYAKGIWEAVKTNDLREVYHLIAVSAVSIVNTTFDHVVSVSSSP 240
           VEKLL+VKD+E  GN SYAK IWEAV+TNDLREVY LIAVSAVSIVN T+DH VSVSSSP
Sbjct: 597 VEKLLLVKDTEASGNNSYAKSIWEAVQTNDLREVYRLIAVSAVSIVNKTYDHAVSVSSSP 656

Query: 241 HLNEEPSGSNRESHSPSLCGRDSDSNESRNNLQGCSLLHLACQNDNQVMLELLLQFGADI 300
           HL+EEPSG NRESH+PS CGR+ DSNESR++LQGCSLLHLACQNDNQVMLELLLQFGADI
Sbjct: 657 HLDEEPSGGNRESHNPSSCGREMDSNESRSSLQGCSLLHLACQNDNQVMLELLLQFGADI 716

Query: 301 NVRDSHGRTPLHQCISQKNNTLAKLLLRRGARPSIKDGGGLSALERAMEMGAITDDELFL 360
           NVRDSHGRTPLHQCISQKNNTLAKLLLRRGAR SIKDGGGLSALERAMEMG+ITDDELFL
Sbjct: 717 NVRDSHGRTPLHQCISQKNNTLAKLLLRRGARQSIKDGGGLSALERAMEMGSITDDELFL 776

BLAST of Lag0041328 vs. ExPASy TrEMBL
Match: A0A6J1FNM8 (ADP-ribosylation factor GTPase-activating protein AGD4-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111447422 PE=4 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 3.6e-181
Identity = 323/366 (88.25%), Postives = 342/366 (93.44%), Query Frame = 0

Query: 1   MDWVNKITGAIASLFNSYFLQQPFLEAKHPESDKNNFDNYNVQTRHQERL-DGSQVDSVS 60
           MDWVNKI GAI SLFNSYFLQQPFLEAKHPE+DKN+FDNYNVQT+HQ+R+ DGSQ DSVS
Sbjct: 412 MDWVNKIMGAITSLFNSYFLQQPFLEAKHPENDKNHFDNYNVQTQHQKRIEDGSQGDSVS 471

Query: 61  KILREIPGNDLCAECGSPEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWE 120
           KILREIPGND+CAECG+PEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWE
Sbjct: 472 KILREIPGNDICAECGTPEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWE 531

Query: 121 PSILDLFRNLGNAYCNSIWEGLLLADIGSDDTQ------ISKPSPKDAIQHRERYIQAKY 180
           PSILDLFRNLGNAYCNSIWEGLLLADIGSD+TQ      I KPSPKDAIQ RE+YI AKY
Sbjct: 532 PSILDLFRNLGNAYCNSIWEGLLLADIGSDNTQHAVVTSIPKPSPKDAIQRREKYIHAKY 591

Query: 181 VEKLLVVKDSEEPGNISYAKGIWEAVKTNDLREVYHLIAVSAVSIVNTTFDHVVSVSSSP 240
           VEKLL+VKD+E  GN SYAK IWEAV+TNDLREVY LIAVSAVSIVN T+DH VSVSSSP
Sbjct: 592 VEKLLLVKDTEASGNNSYAKSIWEAVQTNDLREVYRLIAVSAVSIVNKTYDHAVSVSSSP 651

Query: 241 HLNEEPSGSNRESHSPSLCGRDSDSNESRNNLQGCSLLHLACQNDNQVMLELLLQFGADI 300
           HL+EEPSG NRESH+PS CGR+ DSNESR++LQGCSLLHLACQNDNQVMLELLLQFGADI
Sbjct: 652 HLDEEPSGGNRESHNPSSCGREMDSNESRSSLQGCSLLHLACQNDNQVMLELLLQFGADI 711

Query: 301 NVRDSHGRTPLHQCISQKNNTLAKLLLRRGARPSIKDGGGLSALERAMEMGAITDDELFL 360
           NVRDSHGRTPLHQCISQKNNTLAKLLLRRGAR SIKDGGGLSALERAMEMG+ITDDELFL
Sbjct: 712 NVRDSHGRTPLHQCISQKNNTLAKLLLRRGARQSIKDGGGLSALERAMEMGSITDDELFL 771

BLAST of Lag0041328 vs. ExPASy TrEMBL
Match: A0A6J1JCW8 (ADP-ribosylation factor GTPase-activating protein AGD4-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111483326 PE=4 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 3.0e-180
Identity = 321/366 (87.70%), Postives = 341/366 (93.17%), Query Frame = 0

Query: 1   MDWVNKITGAIASLFNSYFLQQPFLEAKHPESDKNNFDNYNVQTRHQERL-DGSQVDSVS 60
           MDWVNKI GAI SLFNSYFLQQPFLEAKHPE+DKN+FDNYNVQT+HQ+R+ DGSQ DSVS
Sbjct: 412 MDWVNKIMGAITSLFNSYFLQQPFLEAKHPENDKNHFDNYNVQTQHQKRIEDGSQGDSVS 471

Query: 61  KILREIPGNDLCAECGSPEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWE 120
           KILREIPGND+CAECG+PEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWE
Sbjct: 472 KILREIPGNDICAECGTPEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWE 531

Query: 121 PSILDLFRNLGNAYCNSIWEGLLLADIGSDDTQ------ISKPSPKDAIQHRERYIQAKY 180
           PSILDLFRNLGNAYCNSIWEGLLLAD GSD+TQ      I KPSPKDAIQ RE+YIQAKY
Sbjct: 532 PSILDLFRNLGNAYCNSIWEGLLLADTGSDNTQHAVVTSIPKPSPKDAIQRREKYIQAKY 591

Query: 181 VEKLLVVKDSEEPGNISYAKGIWEAVKTNDLREVYHLIAVSAVSIVNTTFDHVVSVSSSP 240
           VEK L+VKD+E  GN SYAK IWEAV+TNDLREVY LIAVSAVSIVN T+DH VSVSSSP
Sbjct: 592 VEKQLLVKDTEASGNNSYAKSIWEAVRTNDLREVYRLIAVSAVSIVNKTYDHAVSVSSSP 651

Query: 241 HLNEEPSGSNRESHSPSLCGRDSDSNESRNNLQGCSLLHLACQNDNQVMLELLLQFGADI 300
           HL+EEPSG NRESH+PS CGR+ DSNESR++LQGCSLLHLACQNDNQVMLELLLQFGADI
Sbjct: 652 HLDEEPSGGNRESHNPSSCGREMDSNESRSSLQGCSLLHLACQNDNQVMLELLLQFGADI 711

Query: 301 NVRDSHGRTPLHQCISQKNNTLAKLLLRRGARPSIKDGGGLSALERAMEMGAITDDELFL 360
           NVRDSHGRTPLHQCISQKNNTLAKLLLRRGAR SIKDGGGLSALERAMEMG+ITDDELFL
Sbjct: 712 NVRDSHGRTPLHQCISQKNNTLAKLLLRRGARQSIKDGGGLSALERAMEMGSITDDELFL 771

BLAST of Lag0041328 vs. ExPASy TrEMBL
Match: A0A6J1JD77 (ADP-ribosylation factor GTPase-activating protein AGD4-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111483326 PE=4 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 3.0e-180
Identity = 321/366 (87.70%), Postives = 341/366 (93.17%), Query Frame = 0

Query: 1   MDWVNKITGAIASLFNSYFLQQPFLEAKHPESDKNNFDNYNVQTRHQERL-DGSQVDSVS 60
           MDWVNKI GAI SLFNSYFLQQPFLEAKHPE+DKN+FDNYNVQT+HQ+R+ DGSQ DSVS
Sbjct: 416 MDWVNKIMGAITSLFNSYFLQQPFLEAKHPENDKNHFDNYNVQTQHQKRIEDGSQGDSVS 475

Query: 61  KILREIPGNDLCAECGSPEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWE 120
           KILREIPGND+CAECG+PEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWE
Sbjct: 476 KILREIPGNDICAECGTPEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKVWE 535

Query: 121 PSILDLFRNLGNAYCNSIWEGLLLADIGSDDTQ------ISKPSPKDAIQHRERYIQAKY 180
           PSILDLFRNLGNAYCNSIWEGLLLAD GSD+TQ      I KPSPKDAIQ RE+YIQAKY
Sbjct: 536 PSILDLFRNLGNAYCNSIWEGLLLADTGSDNTQHAVVTSIPKPSPKDAIQRREKYIQAKY 595

Query: 181 VEKLLVVKDSEEPGNISYAKGIWEAVKTNDLREVYHLIAVSAVSIVNTTFDHVVSVSSSP 240
           VEK L+VKD+E  GN SYAK IWEAV+TNDLREVY LIAVSAVSIVN T+DH VSVSSSP
Sbjct: 596 VEKQLLVKDTEASGNNSYAKSIWEAVRTNDLREVYRLIAVSAVSIVNKTYDHAVSVSSSP 655

Query: 241 HLNEEPSGSNRESHSPSLCGRDSDSNESRNNLQGCSLLHLACQNDNQVMLELLLQFGADI 300
           HL+EEPSG NRESH+PS CGR+ DSNESR++LQGCSLLHLACQNDNQVMLELLLQFGADI
Sbjct: 656 HLDEEPSGGNRESHNPSSCGREMDSNESRSSLQGCSLLHLACQNDNQVMLELLLQFGADI 715

Query: 301 NVRDSHGRTPLHQCISQKNNTLAKLLLRRGARPSIKDGGGLSALERAMEMGAITDDELFL 360
           NVRDSHGRTPLHQCISQKNNTLAKLLLRRGAR SIKDGGGLSALERAMEMG+ITDDELFL
Sbjct: 716 NVRDSHGRTPLHQCISQKNNTLAKLLLRRGARQSIKDGGGLSALERAMEMGSITDDELFL 775

BLAST of Lag0041328 vs. TAIR 10
Match: AT1G10870.1 (ARF-GAP domain 4 )

HSP 1 Score: 406.0 bits (1042), Expect = 3.2e-113
Identity = 211/365 (57.81%), Postives = 264/365 (72.33%), Query Frame = 0

Query: 1   MDWVNKITGAIASLFNSYFLQQP---FLEAKHPESDKNNFDNYNVQTRHQERLDGSQVDS 60
           MDWVNKIT AI +L NS+FLQQ    +L+  +  S   N      Q RH +       D 
Sbjct: 410 MDWVNKITKAIGTLLNSHFLQQSPVRYLDKDNSSSAPANAVVSGDQIRHNDSRQNIG-DD 469

Query: 61  VSKILREIPGNDLCAECGSPEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVKV 120
           VS ILR +PGN+ CAEC +PEP+WASLNLG+LLCI+CSGVHRNLGVHISKVRS+ LDVKV
Sbjct: 470 VSTILRGLPGNNACAECNAPEPDWASLNLGVLLCIQCSGVHRNLGVHISKVRSLSLDVKV 529

Query: 121 WEPSILDLFRNLGNAYCNSIWEGLLLADIGSDD------TQISKPSPKDAIQHRERYIQA 180
           WEP+ILDLFRNLGN YCNS+WEGLL  D   +D        +SKP P+D+   +E+YI  
Sbjct: 530 WEPTILDLFRNLGNVYCNSLWEGLLHLDDDCEDGSALSHASVSKPCPEDSFSVKEKYILG 589

Query: 181 KYVEKLLVVKDSEEPGNISYAKGIWEAVKTNDLREVYHLIAVSA-VSIVNTTFDHVVSVS 240
           KY+EK LV+KD  E  N+S A  IWEAV++ ++RE+Y LI  +  V+I+NT FD +  + 
Sbjct: 590 KYLEKALVIKDESE-ANLSAASRIWEAVQSRNIREIYRLIVTTGDVNIINTKFDDITDID 649

Query: 241 SSPHLNEEPSGSNRESHSPSLCGRDSDSNESRNNLQGCSLLHLACQNDNQVMLELLLQFG 300
           +  H+ +    + ++ H P++C R  +SNE R+ LQGCSLLH+AC   + V+LELLLQFG
Sbjct: 650 AYHHI-DAAEKAVKKRHDPTVCQRIKESNEPRSCLQGCSLLHVACHIGDSVLLELLLQFG 709

Query: 301 ADINVRDSHGRTPLHQCISQKNNTLAKLLLRRGARPSIKDGGGLSALERAMEMGAITDDE 356
           AD+N+RD HGRTPLH CIS  N+  AK+LLRRGARPSI+D GGLS LERAMEMGAITD+E
Sbjct: 710 ADLNIRDYHGRTPLHHCISSGNHKFAKILLRRGARPSIEDDGGLSVLERAMEMGAITDEE 769

BLAST of Lag0041328 vs. TAIR 10
Match: AT1G60860.1 (ARF-GAP domain 2 )

HSP 1 Score: 403.7 bits (1036), Expect = 1.6e-112
Identity = 211/366 (57.65%), Postives = 266/366 (72.68%), Query Frame = 0

Query: 1   MDWVNKITGAIASLFNSYFLQQP---FLEAKHPESDKNNFD-NYNVQTRHQERLDGSQVD 60
           MDWVNKIT AI    NS+FLQQ    +L+ K+  S     +   N +  + +RL+    D
Sbjct: 410 MDWVNKITAAITIRLNSHFLQQSPARYLDKKNTSSGPATENLTLNQKEDYNQRLNVG--D 469

Query: 61  SVSKILREIPGNDLCAECGSPEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDVK 120
            V  ILREIPGN+ CAEC +P+P+WASLNLG+L+CIECSGVHRNLGVHISKVRS+ LDVK
Sbjct: 470 DVLTILREIPGNNTCAECNAPDPDWASLNLGVLMCIECSGVHRNLGVHISKVRSLTLDVK 529

Query: 121 VWEPSILDLFRNLGNAYCNSIWEGLLL-----ADIGSDDT--QISKPSPKDAIQHRERYI 180
           VWEP+ILDLFRNLGN YCNS+WE LL      ++ GS DT   +SKPS +D    +E+YI
Sbjct: 530 VWEPTILDLFRNLGNGYCNSVWEELLHHLDDDSEKGSTDTLASVSKPSSEDWFTLKEKYI 589

Query: 181 QAKYVEKLLVVKDSEEPGNISYAKGIWEAVKTNDLREVYHLIAVSAVSIVNTTFDHVVSV 240
             KY+EK LVVKD  E  N + +  IWEAV++ ++R++Y LI  +  +I+NT FD +  +
Sbjct: 590 NGKYLEKALVVKDERE-ANSTASSRIWEAVQSRNIRDIYRLIVKADANIINTKFDDITDL 649

Query: 241 SSSPHLNEEPSGSNRESHSPSLCGRDSDSNESRNNLQGCSLLHLACQNDNQVMLELLLQF 300
               H + +     ++ H P+ C R  +SNE+RN LQGCSLLH+ACQ+ + ++LELLLQF
Sbjct: 650 DVYHHHHVDAPDEVKKRHDPNACQRIKNSNEARNCLQGCSLLHVACQSGDPILLELLLQF 709

Query: 301 GADINVRDSHGRTPLHQCISQKNNTLAKLLLRRGARPSIKDGGGLSALERAMEMGAITDD 356
           GADIN+RD HGRTPLH CI+  NN  AK+LLRRGARPSI+DGGGLS LERAMEMGAITD+
Sbjct: 710 GADINMRDYHGRTPLHHCIASGNNAFAKVLLRRGARPSIEDGGGLSVLERAMEMGAITDE 769

BLAST of Lag0041328 vs. TAIR 10
Match: AT5G13300.1 (ARF GTPase-activating protein )

HSP 1 Score: 255.4 bits (651), Expect = 6.9e-68
Identity = 169/405 (41.73%), Postives = 227/405 (56.05%), Query Frame = 0

Query: 1   MDWVNKITGAIASLFNSYFLQQPF---------------------LEAKHPESD----KN 60
           MDW+ KITG IASL +S   +Q                        E  HP ++    + 
Sbjct: 419 MDWIEKITGVIASLLSSQVPEQRLPGSPMGSGHHRSASESSSYESSEYDHPTTEEFVCER 478

Query: 61  NFDNYN------VQTRHQERLDGSQVDSVSKILREIPGNDLCAECGSPEPEWASLNLGIL 120
           +F  YN       Q +   R     +D+    LR++ GND CA+CG+PEP+WASLNLG+L
Sbjct: 479 SFLGYNERPSRSFQPQRSIRKGEKPIDA----LRKVCGNDKCADCGAPEPDWASLNLGVL 538

Query: 121 LCIECSGVHRNLGVHISKVRSIILDVKVWEPSILDLFRNLGNAYCNSIWEGLLLA----- 180
           +CIECSGVHRNLGVHISKVRS+ LDVKVWEPS++ LF+ LGN + N++WE LL +     
Sbjct: 539 VCIECSGVHRNLGVHISKVRSLTLDVKVWEPSVISLFQALGNTFANTVWEELLHSRSAIH 598

Query: 181 -DIG---SDDTQI---SKPSPKDAIQHRERYIQAKYVEKLLVVKDSEEPGNISYAKGIWE 240
            D G   SD +++    KPS  D I  +E+YIQAKY EKL V +  +     S A+ +W+
Sbjct: 599 FDPGLTVSDKSRVMVTGKPSYADMISIKEKYIQAKYAEKLFVRRSRDSDFPQSAAQQMWD 658

Query: 241 AVKTNDLREVYHLIAVSAVSIVNTTFDHVVSVS-SSPHLNEEPSGSNRES-----HSPSL 300
           AV  ND + VY LI V+  + VN  +D   S S +   +   P    RE       +  L
Sbjct: 659 AVSGNDKKAVYRLI-VNGDADVNYVYDQTSSSSLTLSRVILVPERPKREDVLLRLRNELL 718

Query: 301 CGRDSDSNESRNNLQGCSLLHLACQNDNQVMLELLLQFGADINVRDSHGRTPLHQCISQK 357
               S SN S     G SLLH AC+  +  M+ELLLQ+GA++N  DS G+TPLH C+ + 
Sbjct: 719 DRTGSSSNISPEGSGGSSLLHCACEKADLGMVELLLQYGANVNASDSSGQTPLHCCLLRG 778

BLAST of Lag0041328 vs. TAIR 10
Match: AT5G61980.1 (ARF-GAP domain 1 )

HSP 1 Score: 236.9 bits (603), Expect = 2.6e-62
Identity = 158/417 (37.89%), Postives = 220/417 (52.76%), Query Frame = 0

Query: 1   MDWVNKITGAIASLFNSYFLQQPFLEAKHPE-------SDKNNF-DNYNVQTR------- 60
           MDW+ KITG IASL +    ++  +     +       SD  +  D Y+++         
Sbjct: 414 MDWIEKITGVIASLLSFQTPERAIMRLSTVDGGDTFSASDSGSLADPYDIEQAESGESTV 473

Query: 61  -------HQERLDG--SQVDSVS-----KILREIPGNDLCAECGSPEPEWASLNLGILLC 120
                  ++ R  G   Q D V       +L  + GN+ CA+CG+PEP+WASLNLG+L+C
Sbjct: 474 EHPMTGGNRSRFSGCLQQHDMVKTEKPIDVLTRVLGNERCADCGAPEPDWASLNLGVLIC 533

Query: 121 IECSGVHRNLGVHISKVRSIILDVKVWEPSILDLFRNLGNAYCNSIWEGLLLADIGSDDT 180
           IECSG+HRNLGVHISKVRS+ LDVKVWEPS+L LF++LGN Y NS+WE LL ++  +   
Sbjct: 534 IECSGIHRNLGVHISKVRSLTLDVKVWEPSVLTLFQSLGNVYVNSVWEELLNSESRTSSA 593

Query: 181 QISKPSPK---------------DAIQHRERYIQAKYVEKLLVVKDSEEPGNISYAKGIW 240
             S  +PK               D I  +E +I AKY E++ V K  +     +  + IW
Sbjct: 594 SRSSGTPKSDRPRKLLVRKPGFNDPISVKELFIHAKYSERIFVRKAIDSQHFQAVFQEIW 653

Query: 241 EAVKTNDLREVY-HLIAVSA-VSIVNTTFDHVVSVSSSPHLN------------EEPSGS 300
           E V+ ND + VY H++   A V+ +     + VS+  S  +             EE    
Sbjct: 654 ENVRANDKKSVYRHIVCSEADVNALRGQASYTVSLPLSKMMQMEETLEAKFKSIEEEFQE 713

Query: 301 NRESHSPSLCGRDSDSNESRNNLQGCSLLHLACQNDNQVMLELLLQFGADINVRDSHGRT 360
           N   +S S    +S   E  +N   CSLLHLAC + +  M+ELLLQ+GA IN  DS GRT
Sbjct: 714 NPAGYSNSRGDGESMVREETSN--DCSLLHLACLSADIGMVELLLQYGAKINATDSKGRT 773

BLAST of Lag0041328 vs. TAIR 10
Match: AT3G17660.1 (ARF-GAP domain 15 )

HSP 1 Score: 97.1 bits (240), Expect = 3.2e-20
Identity = 57/134 (42.54%), Postives = 75/134 (55.97%), Query Frame = 0

Query: 59  SKILREI---PGNDLCAECGSPEPEWASLNLGILLCIECSGVHRNLGVHISKVRSIILDV 118
           SKIL  +   P N  CA+C S  P WAS+NLGI +C++CSG+HR+LGVHIS+VRSI LD 
Sbjct: 16  SKILEALLKHPDNRECADCRSKAPRWASVNLGIFICMQCSGIHRSLGVHISQVRSITLD- 75

Query: 119 KVWEPSILDLFRNLGNAYCNSIWEGLLLADIGSDDTQISKPSPKDAIQHRERYIQAKYVE 178
             W P  +   ++ GNA  N  WE        S+  Q  + S  D       +I+AKY E
Sbjct: 76  -TWLPDQVAFMKSTGNAKGNEYWE--------SELPQHFERSSSDT------FIRAKYSE 133

Query: 179 KLLVVKDSEEPGNI 190
           K  V   + +P  I
Sbjct: 136 KRWVSPGAIQPAPI 133