Lag0041276 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0041276 vs. NCBI nr
Match: XP_038892956.1 (subtilisin-like protease SBT4.14 [Benincasa hispida])

HSP 1 Score: 1209.5 bits (3128), Expect = 0.0e+00
Identity = 616/743 (82.91%), Postives = 667/743 (89.77%), Query Frame = 0

Query: 1   MSISKSSRLLVFVLFFVVAFVAAVEIDEKKKHFIVFLENKPVLNEVDAVETHLNVLKSVK 60
           MSISKSSRLLVF LFF+V  VAA+++D++K HFIVFLENK VLNEVD VETHLNVL SVK
Sbjct: 1   MSISKSSRLLVFALFFIVVCVAALDVDKEKNHFIVFLENKSVLNEVDGVETHLNVLMSVK 60

Query: 61  ESHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHNVIPNKYRKLQTTRSWDFIG 120
           +SH EAKESMVYSY+KSFNAFAAKLTE+EAK LS REDVH+VIPNKYRKLQTTRSWDF+G
Sbjct: 61  KSHAEAKESMVYSYSKSFNAFAAKLTEDEAKKLSTREDVHHVIPNKYRKLQTTRSWDFLG 120

Query: 121 LPINARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCNNKL 180
           L  NARRNTK ESDIVVGLFDTGITPTA+SFKDDGFGPPPKKWKG+CHH+ANFTGCNNKL
Sbjct: 121 LSSNARRNTKHESDIVVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHYANFTGCNNKL 180

Query: 181 IGARYFKLDGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSDLAKGTARGGVPSAR 240
           IGARYFKLDGS DP DILSP+D DGHGTHTSSTATGNA+AGASLS LA+GTARGGVPSAR
Sbjct: 181 IGARYFKLDGSPDPADILSPVDTDGHGTHTSSTATGNAVAGASLSGLAEGTARGGVPSAR 240

Query: 241 VAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGGFGNYSDDSISIGAFHAMKK 300
           V+MYKVCW S+GC+DMDILAAFDAAIHDGVDVISISIGGGGF NYS+DSISIGAFHAMKK
Sbjct: 241 VSMYKVCWASSGCSDMDILAAFDAAIHDGVDVISISIGGGGFNNYSEDSISIGAFHAMKK 300

Query: 301 GIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFVSPLELGNGNNISGVAINTFNPK 360
           GIITVTSAGNSGP+AGS+VNHAPWIVTVAASAIDRKF+SPLELGNG NISGV IN FNPK
Sbjct: 301 GIITVTSAGNSGPLAGSVVNHAPWIVTVAASAIDRKFISPLELGNGKNISGVGINIFNPK 360

Query: 361 EKMYPLVSGRDVARNSESKESASYCAEESLDPNKVKGNLVLCKFMSWGTDSVVKSLGGSG 420
           +KMYPLVSG DVARNSESK+SASYC E+SLDPNKVKGNLV CK M+WG DSV+KSLG +G
Sbjct: 361 QKMYPLVSGGDVARNSESKDSASYCEEDSLDPNKVKGNLVFCKLMTWGADSVIKSLGANG 420

Query: 421 VIIQSDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQFKATAPMVASF 480
           VIIQSDQFLDNADIFMAPAT+VSS +G  I  YIKST+TP AVIY+T+Q KA APMVASF
Sbjct: 421 VIIQSDQFLDNADIFMAPATMVSSSLGTIIHTYIKSTRTPTAVIYKTKQLKAKAPMVASF 480

Query: 481 SSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTL----SLALPWPA 540
           SSRGPNPGSNRILKPDIAAPGVDILA YTPLKSLTGLKGDTQ+SKFTL    S+A P  A
Sbjct: 481 SSRGPNPGSNRILKPDIAAPGVDILASYTPLKSLTGLKGDTQYSKFTLMSGTSMACPHVA 540

Query: 541 LTSP---------PPPPMSSLSTPTARPISRRLNPDGEFAYGAGNLNPSRAKNPGLIYDL 600
             +           P  + S    TARPISRRLNPDGEFAYGAGNLNPSRA +PGLIYD+
Sbjct: 541 AAAAYVKSFHPHWSPAAIRSALITTARPISRRLNPDGEFAYGAGNLNPSRAISPGLIYDV 600

Query: 601 NEMSYIQFLCREGYTGSSIAVLAGTKSINCSTLIPGLGHDSLNYPTIQLSLKSTQRPMTT 660
           NE+SY+QFLC EGYTGSSIAVLAGTKSINCS LIPG GHDSLNYPT QLSLKST++P TT
Sbjct: 601 NEISYVQFLCSEGYTGSSIAVLAGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTRQPTTT 660

Query: 661 VFRRRVTNVGRAVSVYNATVRAPAGVEITVVPTTLSFSRLLQKRSFKIVVKASPLPSTKM 720
            FRRRVTNV   VSV+NAT++AP GVEITV PTTLSFSRLLQKRSFK+VVKASPLPSTKM
Sbjct: 661 TFRRRVTNVAHPVSVFNATIKAPPGVEITVTPTTLSFSRLLQKRSFKVVVKASPLPSTKM 720

Query: 721 VSGSLAWVGARHVVRSPIVVYSP 731
           VSGS+AW+GARHVVRSPIVVYSP
Sbjct: 721 VSGSIAWIGARHVVRSPIVVYSP 743

BLAST of Lag0041276 vs. NCBI nr
Match: XP_011654432.1 (subtilisin-like protease SBT4.14 [Cucumis sativus] >KGN49552.1 hypothetical protein Csa_018364 [Cucumis sativus])

HSP 1 Score: 1174.1 bits (3036), Expect = 0.0e+00
Identity = 600/743 (80.75%), Postives = 655/743 (88.16%), Query Frame = 0

Query: 1   MSISKSSRLLVFVLFFVVAFVAAVEIDEKKKHFIVFLENKPVLNEVDAVETHLNVLKSVK 60
           MSISKSSRLLVF LF VV  VA ++ DE+K H+IVFLENKPVLNEVD VETHLN+L SVK
Sbjct: 1   MSISKSSRLLVFALFIVVGCVAGLDEDEEKNHYIVFLENKPVLNEVDVVETHLNLLMSVK 60

Query: 61  ESHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHNVIPNKYRKLQTTRSWDFIG 120
           +SH EA ESMVYSYTKSFNAFAAKL+++EAK LS R+DVH+VIPNKYRKLQTTRSWDFIG
Sbjct: 61  KSHAEASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSWDFIG 120

Query: 121 LPINARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCNNKL 180
           L  NARR+TK ESDI+VGLFDTGITPTA+SFKDDGFGPPPKKWKG+CHHFANFT CN KL
Sbjct: 121 LSSNARRSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTACNKKL 180

Query: 181 IGARYFKLDGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSDLAKGTARGGVPSAR 240
           IGARYFKLDG+ DP+DILSP+D DGHGTHTSSTATGNAIAGASLS LA+GTARGGVPSAR
Sbjct: 181 IGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGVPSAR 240

Query: 241 VAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGGFGNYSDDSISIGAFHAMKK 300
           VAMYKVCW S+GC+DMDILAAFDAAI DGVDVISISIGGGGF NYSDDSISIGAFHAMKK
Sbjct: 241 VAMYKVCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHAMKK 300

Query: 301 GIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFVSPLELGNGNNISGVAINTFNPK 360
           GIITVTSAGN GP AGS+VNHAPWIVTVAAS+IDRKF+SPLELGNG NISGV IN FNPK
Sbjct: 301 GIITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIFNPK 360

Query: 361 EKMYPLVSGRDVARNSESKESASYCAEESLDPNKVKGNLVLCKFMSWGTDSVVKSLGGSG 420
           +KMYPLVSG DVARNSESK++AS+C E +LDP KVKG+LV CK ++WG DSV+KS+G +G
Sbjct: 361 QKMYPLVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSIGANG 420

Query: 421 VIIQSDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQFKATAPMVASF 480
           VIIQSD+FLDNADIFMAPAT+VSSLVG+ I  YIKST+TP AVIY+T+Q KA APMVASF
Sbjct: 421 VIIQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQLKAKAPMVASF 480

Query: 481 SSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTL----SLALPWPA 540
           SSRGPNPGS+RILKPDIAAPGVDILA YTPLKSLTG KGDTQ+SKFTL    S+A P  A
Sbjct: 481 SSRGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMACPHVA 540

Query: 541 LTSP---------PPPPMSSLSTPTARPISRRLNPDGEFAYGAGNLNPSRAKNPGLIYDL 600
             +           P  + S    TA PISRRLNP+GEFAYGAGNLNPSRA +PGLIYDL
Sbjct: 541 AAAAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGNLNPSRAISPGLIYDL 600

Query: 601 NEMSYIQFLCREGYTGSSIAVLAGTKSINCSTLIPGLGHDSLNYPTIQLSLKSTQRPMTT 660
           NE+SYIQFLC EGYTGSSIAVL+GTKSINCS LIPG GHDSLNYPT QLSLKST +PMTT
Sbjct: 601 NEISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQPMTT 660

Query: 661 VFRRRVTNVGRAVSVYNATVRAPAGVEITVVPTTLSFSRLLQKRSFKIVVKASPLPSTKM 720
            FRRRVTNVG  +SVYNAT+ AP GV ITV P TLSFSRLLQKRSFK+VVKASPLPS KM
Sbjct: 661 TFRRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLPSAKM 720

Query: 721 VSGSLAWVGARHVVRSPIVVYSP 731
           VSGSLAWVGA+HVVRSPIVVYSP
Sbjct: 721 VSGSLAWVGAQHVVRSPIVVYSP 743

BLAST of Lag0041276 vs. NCBI nr
Match: XP_008454753.1 (PREDICTED: subtilisin-like protease SBT4.14 [Cucumis melo] >KAA0056482.1 subtilisin-like protease SBT4.14 [Cucumis melo var. makuwa])

HSP 1 Score: 1161.4 bits (3003), Expect = 0.0e+00
Identity = 596/746 (79.89%), Postives = 656/746 (87.94%), Query Frame = 0

Query: 1   MSISKSSRLLVFVLFFVVAFVAA---VEIDEKKKHFIVFLENKPVLNEVDAVETHLNVLK 60
           MS+SKSSRLLVF LF +V  V      + +E+K HFIVFL+NKPVLNEVDAVETHL+VL 
Sbjct: 1   MSLSKSSRLLVFALFIIVGCVVQGMDRDENEEKNHFIVFLQNKPVLNEVDAVETHLSVLM 60

Query: 61  SVKESHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHNVIPNKYRKLQTTRSWD 120
           SVK+SH EA ESMVYSYTKSFNAFAAKL++ EAK LS R+DVH+VIPN YRKLQTTRSWD
Sbjct: 61  SVKKSHAEASESMVYSYTKSFNAFAAKLSDHEAKLLSTRKDVHHVIPNTYRKLQTTRSWD 120

Query: 121 FIGLPINARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCN 180
           FIGL  NARR TK ESDI+VGLFDTGITPTA+SF+DDGFGPPPKKWKG+CHHFANFT CN
Sbjct: 121 FIGLSSNARRRTKHESDIIVGLFDTGITPTADSFRDDGFGPPPKKWKGTCHHFANFTACN 180

Query: 181 NKLIGARYFKLDGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSDLAKGTARGGVP 240
            KLIGARYFKLDG+ DP+DILSP+D DGHGTHTSSTATGNA+AGASLS LA+GTARGGVP
Sbjct: 181 KKLIGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAVAGASLSGLAEGTARGGVP 240

Query: 241 SARVAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGGFGNYSDDSISIGAFHA 300
           SARVAMYKVCW S+GC+DMDILAAFDAAI DGVDVISISIGGGGF NYSDDSISIGAFHA
Sbjct: 241 SARVAMYKVCWASSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHA 300

Query: 301 MKKGIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFVSPLELGNGNNISGVAINTF 360
           MKKGIITVTSAGN GP+AGS+VNHAPWIVTVAAS+IDRKF+SPLELGNG NISGV IN F
Sbjct: 301 MKKGIITVTSAGNGGPMAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIF 360

Query: 361 NPKEKMYPLVSGRDVARNSESKESASYCAEESLDPNKVKGNLVLCKFMSWGTDSVVKSLG 420
           NPK+KMYPLVSG DVARNSESK++AS+C E+SLDP KVKG+LV CK ++WG DSV+KS+G
Sbjct: 361 NPKKKMYPLVSGGDVARNSESKDTASFCLEDSLDPTKVKGSLVFCKLLTWGADSVIKSVG 420

Query: 421 GSGVIIQSDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQFKATAPMV 480
            +GVIIQSDQFLDNADIFMAPAT+VSS VG  I  YIKST+TP AVIY+TRQ KA APMV
Sbjct: 421 ANGVIIQSDQFLDNADIFMAPATMVSSSVGTIIYTYIKSTRTPTAVIYKTRQLKAKAPMV 480

Query: 481 ASFSSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTL----SLALP 540
           ASFSSRGPNPGS+RILKPDIAAPGV+ILAGYTPLKSLTG KGDTQ+SKFT+    S+A P
Sbjct: 481 ASFSSRGPNPGSHRILKPDIAAPGVNILAGYTPLKSLTGQKGDTQYSKFTIMSGTSMACP 540

Query: 541 WPALTSP---------PPPPMSSLSTPTARPISRRLNPDGEFAYGAGNLNPSRAKNPGLI 600
             A  +           P  + S    T RPISRRLNP+GEFAYGAGNLNPS+A +PGLI
Sbjct: 541 HVAAAAAYVKSFHPLWSPAAIRSALLTTTRPISRRLNPEGEFAYGAGNLNPSKAISPGLI 600

Query: 601 YDLNEMSYIQFLCREGYTGSSIAVLAGTKSINCSTLIPGLGHDSLNYPTIQLSLKSTQRP 660
           YDLNEMSYIQFLC EGYTGSSIAVLAGTKSINCSTLIPG GHDSLNYPT QLSL+ST++P
Sbjct: 601 YDLNEMSYIQFLCSEGYTGSSIAVLAGTKSINCSTLIPGHGHDSLNYPTFQLSLRSTKQP 660

Query: 661 MTTVFRRRVTNVGRAVSVYNATVRAPAGVEITVVPTTLSFSRLLQKRSFKIVVKASPLPS 720
           MTT FRR+VTNVG+ VSVYNAT++AP GV+ITV PTTLSFSRLLQKRSFK+VVKASPL S
Sbjct: 661 MTTTFRRQVTNVGQPVSVYNATIKAPPGVKITVTPTTLSFSRLLQKRSFKVVVKASPLQS 720

Query: 721 TKMVSGSLAWVGARHVVRSPIVVYSP 731
            KMVSGSLAWVG RHVVRSPIVVYSP
Sbjct: 721 AKMVSGSLAWVGDRHVVRSPIVVYSP 746

BLAST of Lag0041276 vs. NCBI nr
Match: XP_023550181.1 (subtilisin-like protease SBT4.14 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1149.4 bits (2972), Expect = 0.0e+00
Identity = 597/743 (80.35%), Postives = 646/743 (86.94%), Query Frame = 0

Query: 1   MSISKSSRLLVFVLFFVVAFVAAVEIDEKKKHFIVFLENKPVLNEVDAVETHLNVLKSVK 60
           M IS SSR+LV VL FVV  V AVEIDE+KKHFIVFLE KP LNE DAVETHLNVL SVK
Sbjct: 1   MLISISSRVLVCVLLFVVGCVGAVEIDEEKKHFIVFLEMKPDLNEFDAVETHLNVLMSVK 60

Query: 61  ESHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHNVIPNKYRKLQTTRSWDFIG 120
           ES+VEA+ESMVYSYTKSFNAFAAKL EEEA  LSKREDVH+VIPN+YRKLQTTRSWDFIG
Sbjct: 61  ESYVEAEESMVYSYTKSFNAFAAKLNEEEALALSKREDVHHVIPNQYRKLQTTRSWDFIG 120

Query: 121 LPINARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCNNKL 180
           LP  ARRN+K E+DI+VGLFDTGITPTA+SFKDDGFGPPPKKWKG+CHHFANFTGCN KL
Sbjct: 121 LPSKARRNSK-ETDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTGCNRKL 180

Query: 181 IGARYFKLDGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSDLAKGTARGGVPSAR 240
           IGARYFKLDG+ DP DILSPIDVDGHGTHTSSTATGNA+AGASLS LAKGTARGGVPSAR
Sbjct: 181 IGARYFKLDGNPDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSAR 240

Query: 241 VAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGGFGNYSDDSISIGAFHAMKK 300
           VAMYKVCW STGCADMDILAAFDAAIHDGVDVISISIGGG FGNYSDDSISIGAFHA+KK
Sbjct: 241 VAMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHALKK 300

Query: 301 GIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFVSPLELGNGNNISGVAINTFNPK 360
           GIITVTSAGN GP  GS+VNHAPWIVTV ASAIDRKF+S LELGNG NISGV IN FNPK
Sbjct: 301 GIITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINIFNPK 360

Query: 361 EKMYPLVSGRDVARNSESKESASYCAEESLDPNKVKGNLVLCKFMSWGTDSVVKSLGGSG 420
           +KMYPLV G DVAR +E++ESAS+CAE+SLDP+KVKG+LV C+ ++WG DSVV +LG +G
Sbjct: 361 KKMYPLVYGGDVARGAENRESASHCAEDSLDPSKVKGSLVFCELITWGVDSVVNALGANG 420

Query: 421 VIIQSDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQFKATAPMVASF 480
            IIQSD++LDNA+IFMAPAT+VSS VG  I +YIKST+TP AVIY+TRQ KA APM ASF
Sbjct: 421 AIIQSDEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASF 480

Query: 481 SSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTL----SLALPWPA 540
           SSRGPNPG+ RILKPDIAAPGVDILAGYTPLKSLTG +GDTQFSKFTL    S+A P  A
Sbjct: 481 SSRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVA 540

Query: 541 LTSP---------PPPPMSSLSTPTARPISRRLNPDGEFAYGAGNLNPSRAKNPGLIYDL 600
             +           P  + S    TA  ISRRLNPDGEFAYGAGNLNPSRA NPGLIYDL
Sbjct: 541 AAAAYVKSFHPLWSPAAIRSALITTASRISRRLNPDGEFAYGAGNLNPSRAINPGLIYDL 600

Query: 601 NEMSYIQFLCREGYTGSSIAVLAGTKSINCSTLIPGLGHDSLNYPTIQLSLKSTQRPMTT 660
           NEMSYIQFLCREGYTGSSI VLAGTKSINCSTLIPG GHDSLNYPT QL LKS +R M+T
Sbjct: 601 NEMSYIQFLCREGYTGSSIGVLAGTKSINCSTLIPGHGHDSLNYPTFQLRLKSPRRAMST 660

Query: 661 VFRRRVTNVGRAVSVYNATVRAPAGVEITVVPTTLSFSRLLQKRSFKIVVKASPLPSTKM 720
           VFRRRVTNVG  VSVYNAT++AP GVEITV P+TLSF RL QKRSFK+ VKA PL S KM
Sbjct: 661 VFRRRVTNVGHPVSVYNATIKAPPGVEITVTPSTLSFLRLQQKRSFKVAVKAGPLASGKM 720

Query: 721 VSGSLAWVGARHVVRSPIVVYSP 731
           VSGS+AW+GARH VRSPIVVYSP
Sbjct: 721 VSGSVAWIGARHAVRSPIVVYSP 742

BLAST of Lag0041276 vs. NCBI nr
Match: XP_022941706.1 (subtilisin-like protease SBT4.14 [Cucurbita moschata])

HSP 1 Score: 1147.5 bits (2967), Expect = 0.0e+00
Identity = 595/743 (80.08%), Postives = 647/743 (87.08%), Query Frame = 0

Query: 1   MSISKSSRLLVFVLFFVVAFVAAVEIDEKKKHFIVFLENKPVLNEVDAVETHLNVLKSVK 60
           M IS SSR+LV VL FV+  V AVEIDE+KKHFIVFLE KP LNE DAVETHLNVL SVK
Sbjct: 1   MLISISSRVLVCVLLFVIGCVGAVEIDEEKKHFIVFLEMKPDLNEFDAVETHLNVLMSVK 60

Query: 61  ESHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHNVIPNKYRKLQTTRSWDFIG 120
           ES+VEA+ESMVYSYTKSFNAFAAKLTE+EA  LSKREDVH+VIPN+YRKLQTTRSWDFI 
Sbjct: 61  ESYVEAEESMVYSYTKSFNAFAAKLTEDEALALSKREDVHHVIPNRYRKLQTTRSWDFIE 120

Query: 121 LPINARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCNNKL 180
           LP  ARRN+K E+DI+VGLFDTGITPTA+SFKDDGFGPPPKKWKG+CHHFANFTGCN KL
Sbjct: 121 LPSKARRNSK-ETDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTGCNQKL 180

Query: 181 IGARYFKLDGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSDLAKGTARGGVPSAR 240
           IGARYFKLDG+ DP DILSPIDVDGHGTHTSSTATGNA+AGASLS LAKGTARGGVPSAR
Sbjct: 181 IGARYFKLDGNPDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSAR 240

Query: 241 VAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGGFGNYSDDSISIGAFHAMKK 300
           VAMYKVCW STGCADMDILAAFDAAIHDGVDVISISIGGG FGNYSDDSISIGAFHA+KK
Sbjct: 241 VAMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHALKK 300

Query: 301 GIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFVSPLELGNGNNISGVAINTFNPK 360
           GIITVTSAGN GP  GS+VNHAPWIVTV ASAIDRKF+S LELGNG NISGV INTFNPK
Sbjct: 301 GIITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINTFNPK 360

Query: 361 EKMYPLVSGRDVARNSESKESASYCAEESLDPNKVKGNLVLCKFMSWGTDSVVKSLGGSG 420
           +KMYPLV G DVAR +E++ESAS+CAE+SLDP+KVKG+LV C+ ++WG DSVV +LG +G
Sbjct: 361 KKMYPLVYGGDVARGAENRESASHCAEDSLDPSKVKGSLVFCELITWGVDSVVNALGANG 420

Query: 421 VIIQSDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQFKATAPMVASF 480
            IIQSD++LDNA+IFMAPAT+VSS VG  I +YIKST+TP AVIY+TRQ KA APM ASF
Sbjct: 421 AIIQSDEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASF 480

Query: 481 SSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTL----SLALPWPA 540
           SSRGPNPG+ RILKPDIAAPGVDILAGYTPLKSLTG +GDTQFSKFTL    S+A P  A
Sbjct: 481 SSRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVA 540

Query: 541 LTSP---------PPPPMSSLSTPTARPISRRLNPDGEFAYGAGNLNPSRAKNPGLIYDL 600
             +           P  + S    TA  ISR+LNPDGEFAYGAGNLNPSRA NPGLIYDL
Sbjct: 541 AAAAYVKSFHPLWSPGAIRSALITTASRISRQLNPDGEFAYGAGNLNPSRAINPGLIYDL 600

Query: 601 NEMSYIQFLCREGYTGSSIAVLAGTKSINCSTLIPGLGHDSLNYPTIQLSLKSTQRPMTT 660
           NEMSYIQFLC EGYTGSSI VLAGTKSINCSTLIPG GHDSLNYPT QL LKS QR M+T
Sbjct: 601 NEMSYIQFLCSEGYTGSSIGVLAGTKSINCSTLIPGHGHDSLNYPTFQLRLKSPQRAMST 660

Query: 661 VFRRRVTNVGRAVSVYNATVRAPAGVEITVVPTTLSFSRLLQKRSFKIVVKASPLPSTKM 720
           VFRRRVTNVG  VSVYNAT++AP GVEITV P+TLSFS+L QKRSFK+ VKA PL S KM
Sbjct: 661 VFRRRVTNVGHPVSVYNATIKAPPGVEITVTPSTLSFSQLQQKRSFKVAVKAGPLASGKM 720

Query: 721 VSGSLAWVGARHVVRSPIVVYSP 731
           VSGS+AW+GARH VRSPIVVYSP
Sbjct: 721 VSGSVAWIGARHAVRSPIVVYSP 742

BLAST of Lag0041276 vs. ExPASy Swiss-Prot
Match: Q9LLL8 (Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana OX=3702 GN=SBT4.14 PE=2 SV=1)

HSP 1 Score: 840.1 bits (2169), Expect = 1.9e-242
Identity = 430/735 (58.50%), Postives = 546/735 (74.29%), Query Frame = 0

Query: 9   LLVFVLFFVVAFVAAVEIDEKKKHFIVFLENKPVLNEVDAVETHLNVLKSVKESHVEAKE 68
           +LV V+ ++    A+ E +  K  +I++L ++P  N  + ++TH+N+L S+  S  EAKE
Sbjct: 14  VLVMVVLWISPRYASAEDEHAKDFYIIYLGDRPD-NTEETIKTHINLLSSLNISQEEAKE 73

Query: 69  SMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHNVIPNKYRKLQTTRSWDFIGLPINARRN 128
             VYSYTK+FNAFAAKL+  EAK + + E+V +V  N+YRKL TT+SWDF+GLP+ A+R+
Sbjct: 74  RKVYSYTKAFNAFAAKLSPHEAKKMMEMEEVVSVSRNQYRKLHTTKSWDFVGLPLTAKRH 133

Query: 129 TKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCNNKLIGARYFKL 188
            K E D+++G+ DTGITP +ESF D G GPPP KWKGSC  + NFTGCNNK+IGA+YFK 
Sbjct: 134 LKAERDVIIGVLDTGITPDSESFLDHGLGPPPAKWKGSCGPYKNFTGCNNKIIGAKYFKH 193

Query: 189 DGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSDLAKGTARGGVPSARVAMYKVCW 248
           DG+    ++ SPID+DGHGTHTSST  G  +A ASL  +A GTARG VPSAR+AMYKVCW
Sbjct: 194 DGNVPAGEVRSPIDIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVPSARLAMYKVCW 253

Query: 249 ESTGCADMDILAAFDAAIHDGVDVISISIGGGGFGNYSDDSISIGAFHAMKKGIITVTSA 308
             +GCADMDILA F+AAIHDGV++ISISI GG   +YS DSIS+G+FHAM+KGI+TV SA
Sbjct: 254 ARSGCADMDILAGFEAAIHDGVEIISISI-GGPIADYSSDSISVGSFHAMRKGILTVASA 313

Query: 309 GNSGPVAGSIVNHAPWIVTVAASAIDRKFVSPLELGNGNNISGVAINTFNPKEKMYPLVS 368
           GN GP +G++ NH PWI+TVAAS IDR F S ++LGNG + SG+ I+ F+PK K YPLVS
Sbjct: 314 GNDGPSSGTVTNHEPWILTVAASGIDRTFKSKIDLGNGKSFSGMGISMFSPKAKSYPLVS 373

Query: 369 GRDVARNSESKESASYCAEESLDPNKVKGNLVLCKFMSWGTDSVVKSLGGSGVIIQSDQF 428
           G D A+N++ K  A YC  +SLD  KVKG +++C+    G +S +KS GG+G II SDQ+
Sbjct: 374 GVDAAKNTDDKYLARYCFSDSLDRKKVKGKVMVCRMGGGGVESTIKSYGGAGAIIVSDQY 433

Query: 429 LDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQFKATAPMVASFSSRGPNPG 488
           LDNA IFMAPAT V+S VGD I  YI ST++  AVI +TRQ    AP VASFSSRGPNPG
Sbjct: 434 LDNAQIFMAPATSVNSSVGDIIYRYINSTRSASAVIQKTRQVTIPAPFVASFSSRGPNPG 493

Query: 489 SNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTL----SLALPWPALTSP---- 548
           S R+LKPDIAAPG+DILA +T  +SLTGL GDTQFSKFT+    S+A P  A  +     
Sbjct: 494 SIRLLKPDIAAPGIDILAAFTLKRSLTGLDGDTQFSKFTILSGTSMACPHVAGVAAYVKS 553

Query: 549 -----PPPPMSSLSTPTARPISRRLNPDGEFAYGAGNLNPSRAKNPGLIYDLNEMSYIQF 608
                 P  + S    +A+PISRR+N D EFAYG G +NP RA +PGL+YD++++SY+QF
Sbjct: 554 FHPDWTPAAIKSAIITSAKPISRRVNKDAEFAYGGGQINPRRAASPGLVYDMDDISYVQF 613

Query: 609 LCREGYTGSSIAVLAGTKSINCSTLIPGLGHDSLNYPTIQLSLKSTQRPMTTVFRRRVTN 668
           LC EGY  +++A L GT+S++CS+++PGLGHDSLNYPTIQL+L+S +     VFRRRVTN
Sbjct: 614 LCGEGYNATTLAPLVGTRSVSCSSIVPGLGHDSLNYPTIQLTLRSAKTSTLAVFRRRVTN 673

Query: 669 VGRAVSVYNATVRAPAGVEITVVPTTLSFSRLLQKRSFKIVVKASPLPSTKMVSGSLAWV 728
           VG   SVY ATVRAP GVEITV P +LSFS+  QKRSFK+VVKA  +   K+VSG L W 
Sbjct: 674 VGPPSSVYTATVRAPKGVEITVEPQSLSFSKASQKRSFKVVVKAKQMTPGKIVSGLLVWK 733

Query: 729 GARHVVRSPIVVYSP 731
             RH VRSPIV+YSP
Sbjct: 734 SPRHSVRSPIVIYSP 746

BLAST of Lag0041276 vs. ExPASy Swiss-Prot
Match: Q9LZS6 (Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana OX=3702 GN=SBT4.15 PE=3 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 1.7e-158
Identity = 339/764 (44.37%), Postives = 473/764 (61.91%), Query Frame = 0

Query: 6   SSRLLVFVLFFVV---AFVAAVEIDE-KKKHFIVFLENKPVLNEVDAVETHLNVLKSVKE 65
           + R+ +F+L F +   A +AA E +  ++K +IV++      + V+A E H N+L +V  
Sbjct: 4   NQRVRLFMLCFCLVNNAVIAATEDENVERKPYIVYMGEATENSLVEAAENHHNLLMTVIG 63

Query: 66  SHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHNVIPNKYRKLQTTRSWDFIGL 125
              +A+E  +YSY K+ N F A+L   EA+ LS+ E V +V  N  R+L TTRSWDF+GL
Sbjct: 64  DESKARELKIYSYGKNINGFVARLFPHEAEKLSREEGVVSVFKNTQRQLHTTRSWDFLGL 123

Query: 126 -PINARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCNNKL 185
                +R+   ES+I+VG+ DTGI   + SF D G GPPP KWKG C    NFT CNNK+
Sbjct: 124 VESKYKRSVGIESNIIVGVLDTGIDVESPSFNDKGVGPPPAKWKGKCVTGNNFTRCNNKV 183

Query: 186 IGARYFKL------DGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSDLAKGTARG 245
           IGA+YF +      DG  D     +  D DGHGTHTSST  G +++ ASL  +A GTARG
Sbjct: 184 IGAKYFHIQSEGLPDGEGD-----TAADHDGHGTHTSSTIAGVSVSSASLFGIANGTARG 243

Query: 246 GVPSARVAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGGFGNYSDDSISIGA 305
           GVPSAR+A YKVCW+S GC DMD+LAAFD AI DGVD+ISISIGG     + +D I+IGA
Sbjct: 244 GVPSARIAAYKVCWDS-GCTDMDMLAAFDEAISDGVDIISISIGGASL-PFFEDPIAIGA 303

Query: 306 FHAMKKGIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFVSPLELGNGNNISGVAI 365
           FHAMK+GI+T  SAGN+GP   ++ N APW++TVAA+++DRKF + ++LGNG   SG+++
Sbjct: 304 FHAMKRGILTTCSAGNNGPGLFTVSNLAPWVMTVAANSLDRKFETVVKLGNGLTASGISL 363

Query: 366 NTFNPKEKMYPLVSGRDVAR-NSESKESASYCAEESLDPNKVKGNLVLCKF-------MS 425
           N FNP++KMYPL SG   +  ++      S C   +L  +KV G +V C+          
Sbjct: 364 NGFNPRKKMYPLTSGSLASNLSAGGYGEPSTCEPGTLGEDKVMGKVVYCEAGREEGGNGG 423

Query: 426 WGTDSVVKSLGGSGVIIQSDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYR 485
            G D VV+SL G+GVI+Q  +  D A   +   + V    G  I  YI STK P+AVI++
Sbjct: 424 QGQDHVVRSLKGAGVIVQLLEPTDMATSTLIAGSYVFFEDGTKITEYINSTKNPQAVIFK 483

Query: 486 TRQFKATAPMVASFSSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKF 545
           T+  K  AP ++SFS+RGP   S  ILKPDI+APG++ILA Y+ L S+TG   D + + F
Sbjct: 484 TKTTKMLAPSISSFSARGPQRISPNILKPDISAPGLNILAAYSKLASVTGYPDDNRRTLF 543

Query: 546 TL----SLALPWPALTSP---------PPPPMSSLSTPTARPISRRLNPDGEFAYGAGNL 605
           ++    S+A P  A  +           P  + S    TA P+  + N + E +YG+G +
Sbjct: 544 SIMSGTSMACPHAAAAAAYVKSFHPDWSPAAIKSALMTTATPMRIKGN-EAELSYGSGQI 603

Query: 606 NPSRAKNPGLIYDLNEMSYIQFLCREGYTGSSIAVLAG-------TKSINCSTLIPGLGH 665
           NP RA +PGL+YD+ E +Y++FLC+EGY  +SI +L G        K  NC  +  GLG 
Sbjct: 604 NPRRAIHPGLVYDITEDAYLRFLCKEGYNSTSIGLLTGDNSNNTTKKEYNCENIKRGLGS 663

Query: 666 DSLNYPTIQLSLKSTQRPMTTVFRRRVTNVGRAVSVYNATVRAPAGVEITVVPTTLSFSR 725
           D LNYP++   + ST+  ++ VF R VTNVG   S Y A V AP G+ + VVP  +SF R
Sbjct: 664 DGLNYPSLHKQVNSTEAKVSEVFYRTVTNVGYGPSTYVARVWAPKGLRVEVVPKVMSFER 723

Query: 726 LLQKRSFKIVVKASPLPSTK-MVSGSLAWVGAR-HVVRSPIVVY 729
             +KR+FK+V+      + K +VS S+ W  +R H+VRSPI+++
Sbjct: 724 PKEKRNFKVVIDGVWDETMKGIVSASVEWDDSRGHLVRSPILLF 759

BLAST of Lag0041276 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 7.1e-149
Identity = 309/744 (41.53%), Postives = 456/744 (61.29%), Query Frame = 0

Query: 1   MSISKSSRLLVFVLFFVVAFVAAVEIDEKKKH-FIVFLENKPVLNEVDAVETHLNVLKSV 60
           MS S   +L  F LFF     + ++ D+  K+ +IV++  K  L + D+   H   +   
Sbjct: 1   MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRK--LEDPDSAHLHHRAMLEQ 60

Query: 61  KESHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHNVIPNKYRKLQTTRSWDFI 120
                 A ES++++Y +SFN FA KLTEEEA+ ++  E V +V  N+  +L TTRSWDF+
Sbjct: 61  VVGSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFL 120

Query: 121 GLPINARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCNNK 180
           G P+   R ++ ES+IVVG+ DTGI P + SF D+GF PPP KWKG+C    NF  CN K
Sbjct: 121 GFPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFR-CNRK 180

Query: 181 LIGARYFKLDGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSDLAKGTARGGVPSA 240
           +IGAR + +     P D+  P D +GHGTHT+STA G  ++ A+L  L  GTARGGVP A
Sbjct: 181 IIGARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLA 240

Query: 241 RVAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGGFGNYSDDSISIGAFHAMK 300
           R+A YKVCW + GC+D DILAA+D AI DGVD+IS+S+GG    +Y  D+I+IG+FHA++
Sbjct: 241 RIAAYKVCW-NDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVE 300

Query: 301 KGIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFVSPLELGNGNNISGVAINTFNP 360
           +GI+T  SAGN GP   +  + +PW+++VAAS +DRKFV+ +++GNG +  GV+INTF+ 
Sbjct: 301 RGILTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD- 360

Query: 361 KEKMYPLVSGRDVARNSESKESASYCAEESLDPNKVKGNLVLCKFMSWGTDSVVKSLGGS 420
             + YPLVSGRD+      K ++ +C ++S++PN +KG +V+C+  S+G     KSL G+
Sbjct: 361 -NQYYPLVSGRDIPNTGFDKSTSRFCTDKSVNPNLLKGKIVVCE-ASFGPHEFFKSLDGA 420

Query: 421 GVIIQSDQFLDNADIFMAPATVVSSLVGDTIA--NYIKSTKTPKAVIYR-TRQFKATAPM 480
             ++ +    D AD +  P++V+     D +A   YI S ++P A I++ T    A+AP+
Sbjct: 421 AGVLMTSNTRDYADSYPLPSSVLDP--NDLLATLRYIYSIRSPGATIFKSTTILNASAPV 480

Query: 481 VASFSSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLSLALPWPA 540
           V SFSSRGPN  +  ++KPDI+ PGV+ILA +  +  + G++ +T F+  +   ++  P 
Sbjct: 481 VVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTLFNIIS-GTSMSCPH 540

Query: 541 LT-----------SPPPPPMSSLSTPTARPISRRLNPDGEFAYGAGNLNPSRAKNPGLIY 600
           +T           +  P  + S    TA P++ R NP  EFAYG+G++NP +A  PGL+Y
Sbjct: 541 ITGIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVY 600

Query: 601 DLNEMSYIQFLCREGYTGSSIAVLAGTKSINCSTLIPGLGHDSLNYPTIQLSLKSTQRPM 660
           D NE  Y++FLC +GY   ++  + G  S  C++   G   D LNYP+  LS+  +Q   
Sbjct: 601 DANESDYVKFLCGQGYNTQAVRRITGDYSA-CTSGNTGRVWD-LNYPSFGLSVSPSQ-TF 660

Query: 661 TTVFRRRVTNVGRAVSVYNATVRAPAGVEITVVPTTLSFSRLLQKRSFKIVVKASPLPST 720
              F R +T+V    S Y A + AP G+ I+V P  LSF+ L  ++SF + V+ S     
Sbjct: 661 NQYFNRTLTSVAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGS--IKG 720

Query: 721 KMVSGSLAWVGARHVVRSPIVVYS 730
            +VS SL W    H VRSPI + S
Sbjct: 721 FVVSASLVWSDGVHYVRSPITITS 729

BLAST of Lag0041276 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 495.7 bits (1275), Expect = 8.7e-139
Identity = 301/737 (40.84%), Postives = 439/737 (59.57%), Query Frame = 0

Query: 10  LVFVLFFVVAFVAAVEIDEKKKHFIVFLENKPVLNEVDAVETHLNVLKSVKESHVEAKES 69
           L+ + F     V+A +  +    +IV++   P + +      HL++L+ +  + + A   
Sbjct: 10  LICLAFIFTRDVSANDYRQASSVYIVYMGTLPEI-KYSPPSHHLSILQKLVGT-IAASHL 69

Query: 70  MVYSYTKSFNAFAAKLTEEEAKTLSKREDVHNVIPNKYRKLQTTRSWDFIGLPINARRNT 129
           +V SY +SFN FAA L++ E++ L   ++V +V P+K  +L TTRSWDF+G    ARR +
Sbjct: 70  LVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRES 129

Query: 130 KQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCNNKLIGARYFKLD 189
            +ESD++VG+ D+GI P +ESF D+GFGPPPKKWKGSC     F  CNNKLIGAR++   
Sbjct: 130 VKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKF-ACNNKLIGARFYNKF 189

Query: 190 GSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSDLAKGTARGGVPSARVAMYKVCWE 249
                    S  D +GHGTHT+STA GNA+  AS   LA+GTARGGVPSAR+A YKVC+ 
Sbjct: 190 AD-------SARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCFN 249

Query: 250 STGCADMDILAAFDAAIHDGVDVISISIGGGGFGNYSDDSISIGAFHAMKKGIITVTSAG 309
              C D+DILAAFD AI DGVDVISISI      N  + S++IG+FHAM +GIIT  SAG
Sbjct: 250 R--CNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAG 309

Query: 310 NSGPVAGSIVNHAPWIVTVAASAIDRKFVSPLELGNGNNISGVAINTFNPKEKMYPLVSG 369
           N+GP  GS+ N +PW++TVAAS  DR+F+  + LGNG  ++G+++NTFN     +P+V G
Sbjct: 310 NNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVYG 369

Query: 370 RDVARNSESKESASYCAEESLDPNKVKGNLVLCKFMSWGTDSVVKSLGGSGVIIQSDQFL 429
           ++V+RN  S+  A YC+   +D   VKG +VLC       ++ +   G  GVI+Q+    
Sbjct: 370 QNVSRNC-SQAQAGYCSSGCVDSELVKGKIVLCDDFLGYREAYL--AGAIGVIVQNTLLP 429

Query: 430 DNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQF-KATAPMVASFSSRGPNPG 489
           D+A +   PA+ +      +I +YI+S + P+A I RT +     AP V SFSSRGP+  
Sbjct: 430 DSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFV 489

Query: 490 SNRILKPDIAAPGVDILAGYTPLKSLTGL-----KGDTQFSKFT-LSLALPWPALTSP-- 549
              +LKPD++APG++ILA ++P+ S +       K   ++S  +  S+A P  A  +   
Sbjct: 490 IQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYV 549

Query: 550 -------PPPPMSSLSTPTARPISRRLNPDGEFAYGAGNLNPSRAKNPGLIYDLNEMSYI 609
                   P  + S    TA P++ + NP+ EFAYG+G +NP++A +PGL+Y++    Y+
Sbjct: 550 KSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYL 609

Query: 610 QFLCREGYTGSSIAVLAGTKSINCSTLIPGLGHDSLNYPTIQLSLKSTQRPMTTVFRRRV 669
           + LC EG+  +++   +G +++ CS          LNYPT+   + S   P    F+R V
Sbjct: 610 KMLCAEGFDSTTLTTTSG-QNVTCSERTE---VKDLNYPTMTTFVSSLD-PFNVTFKRTV 669

Query: 670 TNVGRAVSVYNAT-VRAPAGVEITVVPTTLSFSRLLQKRSFKIVVKASPLPSTKMVSGSL 729
           TNVG   S Y A+ V     ++I++ P  L F  L +K+SF + +    L     VS S+
Sbjct: 670 TNVGFPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSSV 726

BLAST of Lag0041276 vs. ExPASy Swiss-Prot
Match: Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 5.7e-138
Identity = 311/749 (41.52%), Postives = 453/749 (60.48%), Query Frame = 0

Query: 2   SISKSSRLLVFVLFFVVAFVAAVEIDEKKKHFIVFLENKPVLNEVDAVETHLNVLKSVK- 61
           +++ SS LL  +L   ++ V+AV  D  K+ +IV++ +     +      H+N+L+ V  
Sbjct: 3   TLAASSSLLSCLLVLFLSSVSAVTDD--KQVYIVYMGSLSSRADYTPTSDHMNILQEVTG 62

Query: 62  ESHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHNVIPNKYRKLQTTRSWDFIG 121
           ES +E +  +V SY +SFN FAA+LTE E + ++K   V +V PNK  +LQTT SWDF+G
Sbjct: 63  ESSIEGR--LVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMG 122

Query: 122 LP--INARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCNN 181
           L   I  +RN   ESD ++G+ D+GITP ++SF D GFGPPP+KWKG C    NFT CNN
Sbjct: 123 LKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFT-CNN 182

Query: 182 KLIGARYFKLDGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSDLAKGTARGGVPS 241
           KLIGAR +  +G+          D+DGHGTHT+STA GNA+  AS   +  GT RGGVP+
Sbjct: 183 KLIGARDYTSEGTR---------DMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPA 242

Query: 242 ARVAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGGFGNYSDDSISIGAFHAM 301
           +RVA YKVC   TGC+   +L+AFD AI DGVD+I+ISIG      + +D I+IGAFHAM
Sbjct: 243 SRVAAYKVC-TPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAM 302

Query: 302 KKGIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFVSPLELGNGNNISGVAINTFN 361
            KG++TV SAGNSGP   S+   APWI+TVAAS  +R FV+ + LGNG  + G ++N + 
Sbjct: 303 AKGVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYE 362

Query: 362 PKEKMYPLVSGRDVARNSESKESASYCAEESLDPNKVKGNLVLCKFMSWGTDSVVKSLGG 421
            K K YPLV G+  A ++   ESA  C    +D ++VKG +++C     G   +V+S+G 
Sbjct: 363 MKGKDYPLVYGKSAASSACDAESAGLCELSCVDKSRVKGKILVCG--GPGGLKIVESVGA 422

Query: 422 SGVIIQSDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQ-FKATAPMV 481
            G+I ++ +  D A I   PA  + +   +++ +Y++ST +P+A++ +T   F  T+P++
Sbjct: 423 VGLIYRTPK-PDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVI 482

Query: 482 ASFSSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTL----SLALP 541
           ASFSSRGPN  +  ILKPDI APGV+ILA Y+P    +  + DT+  K+++    S++ P
Sbjct: 483 ASFSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPS--QDDTRHVKYSVLSGTSMSCP 542

Query: 542 WPALTSP---------PPPPMSSLSTPTARPISRRLN--PDGEFAYGAGNLNPSRAKNPG 601
             A  +           P  + S    TA P++         EFAYG+G+++P  A NPG
Sbjct: 543 HVAGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPG 602

Query: 602 LIYDLNEMSYIQFLCREGYTGSSIAVLAGTKSINCSTLIPGLGHDSLNYPTIQLSLKSTQ 661
           L+Y+L++  +I FLC   YT   + V++G +++ CS     L   +LNYP++   L  + 
Sbjct: 603 LVYELDKSDHIAFLCGMNYTSQVLKVISG-ETVTCSEAKKILPR-NLNYPSMSAKLSGSG 662

Query: 662 RPMTTVFRRRVTNVGRAVSVYNATVRAPAG--VEITVVPTTLSFSRLLQKRSFKIVVKAS 721
              T  F R +TNVG   S Y + V A  G  +++ + P+ LSF  + +K+SF + V  S
Sbjct: 663 TTFTVTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGS 722

Query: 722 PLPSTKMVSGSLAWVGARHVVRSPIVVYS 730
            L S    S +L W    H VRSPIVVY+
Sbjct: 723 NLDSEVPSSANLIWSDGTHNVRSPIVVYT 729

BLAST of Lag0041276 vs. ExPASy TrEMBL
Match: A0A0A0KME6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G000010 PE=3 SV=1)

HSP 1 Score: 1174.1 bits (3036), Expect = 0.0e+00
Identity = 600/743 (80.75%), Postives = 655/743 (88.16%), Query Frame = 0

Query: 1   MSISKSSRLLVFVLFFVVAFVAAVEIDEKKKHFIVFLENKPVLNEVDAVETHLNVLKSVK 60
           MSISKSSRLLVF LF VV  VA ++ DE+K H+IVFLENKPVLNEVD VETHLN+L SVK
Sbjct: 1   MSISKSSRLLVFALFIVVGCVAGLDEDEEKNHYIVFLENKPVLNEVDVVETHLNLLMSVK 60

Query: 61  ESHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHNVIPNKYRKLQTTRSWDFIG 120
           +SH EA ESMVYSYTKSFNAFAAKL+++EAK LS R+DVH+VIPNKYRKLQTTRSWDFIG
Sbjct: 61  KSHAEASESMVYSYTKSFNAFAAKLSDDEAKLLSTRKDVHHVIPNKYRKLQTTRSWDFIG 120

Query: 121 LPINARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCNNKL 180
           L  NARR+TK ESDI+VGLFDTGITPTA+SFKDDGFGPPPKKWKG+CHHFANFT CN KL
Sbjct: 121 LSSNARRSTKHESDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTACNKKL 180

Query: 181 IGARYFKLDGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSDLAKGTARGGVPSAR 240
           IGARYFKLDG+ DP+DILSP+D DGHGTHTSSTATGNAIAGASLS LA+GTARGGVPSAR
Sbjct: 181 IGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGVPSAR 240

Query: 241 VAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGGFGNYSDDSISIGAFHAMKK 300
           VAMYKVCW S+GC+DMDILAAFDAAI DGVDVISISIGGGGF NYSDDSISIGAFHAMKK
Sbjct: 241 VAMYKVCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHAMKK 300

Query: 301 GIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFVSPLELGNGNNISGVAINTFNPK 360
           GIITVTSAGN GP AGS+VNHAPWIVTVAAS+IDRKF+SPLELGNG NISGV IN FNPK
Sbjct: 301 GIITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIFNPK 360

Query: 361 EKMYPLVSGRDVARNSESKESASYCAEESLDPNKVKGNLVLCKFMSWGTDSVVKSLGGSG 420
           +KMYPLVSG DVARNSESK++AS+C E +LDP KVKG+LV CK ++WG DSV+KS+G +G
Sbjct: 361 QKMYPLVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSIGANG 420

Query: 421 VIIQSDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQFKATAPMVASF 480
           VIIQSD+FLDNADIFMAPAT+VSSLVG+ I  YIKST+TP AVIY+T+Q KA APMVASF
Sbjct: 421 VIIQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQLKAKAPMVASF 480

Query: 481 SSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTL----SLALPWPA 540
           SSRGPNPGS+RILKPDIAAPGVDILA YTPLKSLTG KGDTQ+SKFTL    S+A P  A
Sbjct: 481 SSRGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMACPHVA 540

Query: 541 LTSP---------PPPPMSSLSTPTARPISRRLNPDGEFAYGAGNLNPSRAKNPGLIYDL 600
             +           P  + S    TA PISRRLNP+GEFAYGAGNLNPSRA +PGLIYDL
Sbjct: 541 AAAAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGNLNPSRAISPGLIYDL 600

Query: 601 NEMSYIQFLCREGYTGSSIAVLAGTKSINCSTLIPGLGHDSLNYPTIQLSLKSTQRPMTT 660
           NE+SYIQFLC EGYTGSSIAVL+GTKSINCS LIPG GHDSLNYPT QLSLKST +PMTT
Sbjct: 601 NEISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQPMTT 660

Query: 661 VFRRRVTNVGRAVSVYNATVRAPAGVEITVVPTTLSFSRLLQKRSFKIVVKASPLPSTKM 720
            FRRRVTNVG  +SVYNAT+ AP GV ITV P TLSFSRLLQKRSFK+VVKASPLPS KM
Sbjct: 661 TFRRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLPSAKM 720

Query: 721 VSGSLAWVGARHVVRSPIVVYSP 731
           VSGSLAWVGA+HVVRSPIVVYSP
Sbjct: 721 VSGSLAWVGAQHVVRSPIVVYSP 743

BLAST of Lag0041276 vs. ExPASy TrEMBL
Match: A0A5A7UKY8 (Subtilisin-like protease SBT4.14 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold186G002590 PE=3 SV=1)

HSP 1 Score: 1161.4 bits (3003), Expect = 0.0e+00
Identity = 596/746 (79.89%), Postives = 656/746 (87.94%), Query Frame = 0

Query: 1   MSISKSSRLLVFVLFFVVAFVAA---VEIDEKKKHFIVFLENKPVLNEVDAVETHLNVLK 60
           MS+SKSSRLLVF LF +V  V      + +E+K HFIVFL+NKPVLNEVDAVETHL+VL 
Sbjct: 1   MSLSKSSRLLVFALFIIVGCVVQGMDRDENEEKNHFIVFLQNKPVLNEVDAVETHLSVLM 60

Query: 61  SVKESHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHNVIPNKYRKLQTTRSWD 120
           SVK+SH EA ESMVYSYTKSFNAFAAKL++ EAK LS R+DVH+VIPN YRKLQTTRSWD
Sbjct: 61  SVKKSHAEASESMVYSYTKSFNAFAAKLSDHEAKLLSTRKDVHHVIPNTYRKLQTTRSWD 120

Query: 121 FIGLPINARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCN 180
           FIGL  NARR TK ESDI+VGLFDTGITPTA+SF+DDGFGPPPKKWKG+CHHFANFT CN
Sbjct: 121 FIGLSSNARRRTKHESDIIVGLFDTGITPTADSFRDDGFGPPPKKWKGTCHHFANFTACN 180

Query: 181 NKLIGARYFKLDGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSDLAKGTARGGVP 240
            KLIGARYFKLDG+ DP+DILSP+D DGHGTHTSSTATGNA+AGASLS LA+GTARGGVP
Sbjct: 181 KKLIGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAVAGASLSGLAEGTARGGVP 240

Query: 241 SARVAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGGFGNYSDDSISIGAFHA 300
           SARVAMYKVCW S+GC+DMDILAAFDAAI DGVDVISISIGGGGF NYSDDSISIGAFHA
Sbjct: 241 SARVAMYKVCWASSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHA 300

Query: 301 MKKGIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFVSPLELGNGNNISGVAINTF 360
           MKKGIITVTSAGN GP+AGS+VNHAPWIVTVAAS+IDRKF+SPLELGNG NISGV IN F
Sbjct: 301 MKKGIITVTSAGNGGPMAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIF 360

Query: 361 NPKEKMYPLVSGRDVARNSESKESASYCAEESLDPNKVKGNLVLCKFMSWGTDSVVKSLG 420
           NPK+KMYPLVSG DVARNSESK++AS+C E+SLDP KVKG+LV CK ++WG DSV+KS+G
Sbjct: 361 NPKKKMYPLVSGGDVARNSESKDTASFCLEDSLDPTKVKGSLVFCKLLTWGADSVIKSVG 420

Query: 421 GSGVIIQSDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQFKATAPMV 480
            +GVIIQSDQFLDNADIFMAPAT+VSS VG  I  YIKST+TP AVIY+TRQ KA APMV
Sbjct: 421 ANGVIIQSDQFLDNADIFMAPATMVSSSVGTIIYTYIKSTRTPTAVIYKTRQLKAKAPMV 480

Query: 481 ASFSSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTL----SLALP 540
           ASFSSRGPNPGS+RILKPDIAAPGV+ILAGYTPLKSLTG KGDTQ+SKFT+    S+A P
Sbjct: 481 ASFSSRGPNPGSHRILKPDIAAPGVNILAGYTPLKSLTGQKGDTQYSKFTIMSGTSMACP 540

Query: 541 WPALTSP---------PPPPMSSLSTPTARPISRRLNPDGEFAYGAGNLNPSRAKNPGLI 600
             A  +           P  + S    T RPISRRLNP+GEFAYGAGNLNPS+A +PGLI
Sbjct: 541 HVAAAAAYVKSFHPLWSPAAIRSALLTTTRPISRRLNPEGEFAYGAGNLNPSKAISPGLI 600

Query: 601 YDLNEMSYIQFLCREGYTGSSIAVLAGTKSINCSTLIPGLGHDSLNYPTIQLSLKSTQRP 660
           YDLNEMSYIQFLC EGYTGSSIAVLAGTKSINCSTLIPG GHDSLNYPT QLSL+ST++P
Sbjct: 601 YDLNEMSYIQFLCSEGYTGSSIAVLAGTKSINCSTLIPGHGHDSLNYPTFQLSLRSTKQP 660

Query: 661 MTTVFRRRVTNVGRAVSVYNATVRAPAGVEITVVPTTLSFSRLLQKRSFKIVVKASPLPS 720
           MTT FRR+VTNVG+ VSVYNAT++AP GV+ITV PTTLSFSRLLQKRSFK+VVKASPL S
Sbjct: 661 MTTTFRRQVTNVGQPVSVYNATIKAPPGVKITVTPTTLSFSRLLQKRSFKVVVKASPLQS 720

Query: 721 TKMVSGSLAWVGARHVVRSPIVVYSP 731
            KMVSGSLAWVG RHVVRSPIVVYSP
Sbjct: 721 AKMVSGSLAWVGDRHVVRSPIVVYSP 746

BLAST of Lag0041276 vs. ExPASy TrEMBL
Match: A0A1S3C036 (subtilisin-like protease SBT4.14 OS=Cucumis melo OX=3656 GN=LOC103495082 PE=3 SV=1)

HSP 1 Score: 1161.4 bits (3003), Expect = 0.0e+00
Identity = 596/746 (79.89%), Postives = 656/746 (87.94%), Query Frame = 0

Query: 1   MSISKSSRLLVFVLFFVVAFVAA---VEIDEKKKHFIVFLENKPVLNEVDAVETHLNVLK 60
           MS+SKSSRLLVF LF +V  V      + +E+K HFIVFL+NKPVLNEVDAVETHL+VL 
Sbjct: 1   MSLSKSSRLLVFALFIIVGCVVQGMDRDENEEKNHFIVFLQNKPVLNEVDAVETHLSVLM 60

Query: 61  SVKESHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHNVIPNKYRKLQTTRSWD 120
           SVK+SH EA ESMVYSYTKSFNAFAAKL++ EAK LS R+DVH+VIPN YRKLQTTRSWD
Sbjct: 61  SVKKSHAEASESMVYSYTKSFNAFAAKLSDHEAKLLSTRKDVHHVIPNTYRKLQTTRSWD 120

Query: 121 FIGLPINARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCN 180
           FIGL  NARR TK ESDI+VGLFDTGITPTA+SF+DDGFGPPPKKWKG+CHHFANFT CN
Sbjct: 121 FIGLSSNARRRTKHESDIIVGLFDTGITPTADSFRDDGFGPPPKKWKGTCHHFANFTACN 180

Query: 181 NKLIGARYFKLDGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSDLAKGTARGGVP 240
            KLIGARYFKLDG+ DP+DILSP+D DGHGTHTSSTATGNA+AGASLS LA+GTARGGVP
Sbjct: 181 KKLIGARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAVAGASLSGLAEGTARGGVP 240

Query: 241 SARVAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGGFGNYSDDSISIGAFHA 300
           SARVAMYKVCW S+GC+DMDILAAFDAAI DGVDVISISIGGGGF NYSDDSISIGAFHA
Sbjct: 241 SARVAMYKVCWASSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHA 300

Query: 301 MKKGIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFVSPLELGNGNNISGVAINTF 360
           MKKGIITVTSAGN GP+AGS+VNHAPWIVTVAAS+IDRKF+SPLELGNG NISGV IN F
Sbjct: 301 MKKGIITVTSAGNGGPMAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIF 360

Query: 361 NPKEKMYPLVSGRDVARNSESKESASYCAEESLDPNKVKGNLVLCKFMSWGTDSVVKSLG 420
           NPK+KMYPLVSG DVARNSESK++AS+C E+SLDP KVKG+LV CK ++WG DSV+KS+G
Sbjct: 361 NPKKKMYPLVSGGDVARNSESKDTASFCLEDSLDPTKVKGSLVFCKLLTWGADSVIKSVG 420

Query: 421 GSGVIIQSDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQFKATAPMV 480
            +GVIIQSDQFLDNADIFMAPAT+VSS VG  I  YIKST+TP AVIY+TRQ KA APMV
Sbjct: 421 ANGVIIQSDQFLDNADIFMAPATMVSSSVGTIIYTYIKSTRTPTAVIYKTRQLKAKAPMV 480

Query: 481 ASFSSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTL----SLALP 540
           ASFSSRGPNPGS+RILKPDIAAPGV+ILAGYTPLKSLTG KGDTQ+SKFT+    S+A P
Sbjct: 481 ASFSSRGPNPGSHRILKPDIAAPGVNILAGYTPLKSLTGQKGDTQYSKFTIMSGTSMACP 540

Query: 541 WPALTSP---------PPPPMSSLSTPTARPISRRLNPDGEFAYGAGNLNPSRAKNPGLI 600
             A  +           P  + S    T RPISRRLNP+GEFAYGAGNLNPS+A +PGLI
Sbjct: 541 HVAAAAAYVKSFHPLWSPAAIRSALLTTTRPISRRLNPEGEFAYGAGNLNPSKAISPGLI 600

Query: 601 YDLNEMSYIQFLCREGYTGSSIAVLAGTKSINCSTLIPGLGHDSLNYPTIQLSLKSTQRP 660
           YDLNEMSYIQFLC EGYTGSSIAVLAGTKSINCSTLIPG GHDSLNYPT QLSL+ST++P
Sbjct: 601 YDLNEMSYIQFLCSEGYTGSSIAVLAGTKSINCSTLIPGHGHDSLNYPTFQLSLRSTKQP 660

Query: 661 MTTVFRRRVTNVGRAVSVYNATVRAPAGVEITVVPTTLSFSRLLQKRSFKIVVKASPLPS 720
           MTT FRR+VTNVG+ VSVYNAT++AP GV+ITV PTTLSFSRLLQKRSFK+VVKASPL S
Sbjct: 661 MTTTFRRQVTNVGQPVSVYNATIKAPPGVKITVTPTTLSFSRLLQKRSFKVVVKASPLQS 720

Query: 721 TKMVSGSLAWVGARHVVRSPIVVYSP 731
            KMVSGSLAWVG RHVVRSPIVVYSP
Sbjct: 721 AKMVSGSLAWVGDRHVVRSPIVVYSP 746

BLAST of Lag0041276 vs. ExPASy TrEMBL
Match: A0A6J1FLU9 (subtilisin-like protease SBT4.14 OS=Cucurbita moschata OX=3662 GN=LOC111446986 PE=3 SV=1)

HSP 1 Score: 1147.5 bits (2967), Expect = 0.0e+00
Identity = 595/743 (80.08%), Postives = 647/743 (87.08%), Query Frame = 0

Query: 1   MSISKSSRLLVFVLFFVVAFVAAVEIDEKKKHFIVFLENKPVLNEVDAVETHLNVLKSVK 60
           M IS SSR+LV VL FV+  V AVEIDE+KKHFIVFLE KP LNE DAVETHLNVL SVK
Sbjct: 1   MLISISSRVLVCVLLFVIGCVGAVEIDEEKKHFIVFLEMKPDLNEFDAVETHLNVLMSVK 60

Query: 61  ESHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHNVIPNKYRKLQTTRSWDFIG 120
           ES+VEA+ESMVYSYTKSFNAFAAKLTE+EA  LSKREDVH+VIPN+YRKLQTTRSWDFI 
Sbjct: 61  ESYVEAEESMVYSYTKSFNAFAAKLTEDEALALSKREDVHHVIPNRYRKLQTTRSWDFIE 120

Query: 121 LPINARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCNNKL 180
           LP  ARRN+K E+DI+VGLFDTGITPTA+SFKDDGFGPPPKKWKG+CHHFANFTGCN KL
Sbjct: 121 LPSKARRNSK-ETDIIVGLFDTGITPTADSFKDDGFGPPPKKWKGTCHHFANFTGCNQKL 180

Query: 181 IGARYFKLDGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSDLAKGTARGGVPSAR 240
           IGARYFKLDG+ DP DILSPIDVDGHGTHTSSTATGNA+AGASLS LAKGTARGGVPSAR
Sbjct: 181 IGARYFKLDGNPDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSAR 240

Query: 241 VAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGGFGNYSDDSISIGAFHAMKK 300
           VAMYKVCW STGCADMDILAAFDAAIHDGVDVISISIGGG FGNYSDDSISIGAFHA+KK
Sbjct: 241 VAMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHALKK 300

Query: 301 GIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFVSPLELGNGNNISGVAINTFNPK 360
           GIITVTSAGN GP  GS+VNHAPWIVTV ASAIDRKF+S LELGNG NISGV INTFNPK
Sbjct: 301 GIITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINTFNPK 360

Query: 361 EKMYPLVSGRDVARNSESKESASYCAEESLDPNKVKGNLVLCKFMSWGTDSVVKSLGGSG 420
           +KMYPLV G DVAR +E++ESAS+CAE+SLDP+KVKG+LV C+ ++WG DSVV +LG +G
Sbjct: 361 KKMYPLVYGGDVARGAENRESASHCAEDSLDPSKVKGSLVFCELITWGVDSVVNALGANG 420

Query: 421 VIIQSDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQFKATAPMVASF 480
            IIQSD++LDNA+IFMAPAT+VSS VG  I +YIKST+TP AVIY+TRQ KA APM ASF
Sbjct: 421 AIIQSDEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASF 480

Query: 481 SSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTL----SLALPWPA 540
           SSRGPNPG+ RILKPDIAAPGVDILAGYTPLKSLTG +GDTQFSKFTL    S+A P  A
Sbjct: 481 SSRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVA 540

Query: 541 LTSP---------PPPPMSSLSTPTARPISRRLNPDGEFAYGAGNLNPSRAKNPGLIYDL 600
             +           P  + S    TA  ISR+LNPDGEFAYGAGNLNPSRA NPGLIYDL
Sbjct: 541 AAAAYVKSFHPLWSPGAIRSALITTASRISRQLNPDGEFAYGAGNLNPSRAINPGLIYDL 600

Query: 601 NEMSYIQFLCREGYTGSSIAVLAGTKSINCSTLIPGLGHDSLNYPTIQLSLKSTQRPMTT 660
           NEMSYIQFLC EGYTGSSI VLAGTKSINCSTLIPG GHDSLNYPT QL LKS QR M+T
Sbjct: 601 NEMSYIQFLCSEGYTGSSIGVLAGTKSINCSTLIPGHGHDSLNYPTFQLRLKSPQRAMST 660

Query: 661 VFRRRVTNVGRAVSVYNATVRAPAGVEITVVPTTLSFSRLLQKRSFKIVVKASPLPSTKM 720
           VFRRRVTNVG  VSVYNAT++AP GVEITV P+TLSFS+L QKRSFK+ VKA PL S KM
Sbjct: 661 VFRRRVTNVGHPVSVYNATIKAPPGVEITVTPSTLSFSQLQQKRSFKVAVKAGPLASGKM 720

Query: 721 VSGSLAWVGARHVVRSPIVVYSP 731
           VSGS+AW+GARH VRSPIVVYSP
Sbjct: 721 VSGSVAWIGARHAVRSPIVVYSP 742

BLAST of Lag0041276 vs. ExPASy TrEMBL
Match: A0A6J1IN55 (subtilisin-like protease SBT4.14 OS=Cucurbita maxima OX=3661 GN=LOC111477941 PE=3 SV=1)

HSP 1 Score: 1135.9 bits (2937), Expect = 0.0e+00
Identity = 591/743 (79.54%), Postives = 644/743 (86.68%), Query Frame = 0

Query: 1   MSISKSSRLLVFVLFFVVAFVAAVEIDEKKKHFIVFLENKPVLNEVDAVETHLNVLKSVK 60
           M IS SSR+LV VL FVV  V AVEIDE+KKHFIVFLE KP LNE DAVETHLNVL SVK
Sbjct: 1   MLISISSRVLVCVLLFVVGCVDAVEIDEEKKHFIVFLEMKPGLNEFDAVETHLNVLMSVK 60

Query: 61  ESHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHNVIPNKYRKLQTTRSWDFIG 120
           ES+VEA+ESMVYSYTKSFNAFAAKLTEEEA  LSKREDVH+VIPN+YRKLQTTRSWDFIG
Sbjct: 61  ESYVEAEESMVYSYTKSFNAFAAKLTEEEALALSKREDVHHVIPNRYRKLQTTRSWDFIG 120

Query: 121 LPINARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCNNKL 180
           L  +A+RN+K E+DI+VGLFDTGITPTA+SFKDDGFGPPP KWKG+CHHFANFTGCN KL
Sbjct: 121 LSSHAKRNSK-ETDIIVGLFDTGITPTADSFKDDGFGPPPTKWKGTCHHFANFTGCNKKL 180

Query: 181 IGARYFKLDGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSDLAKGTARGGVPSAR 240
           IGARYFKLDG++DP DILSPIDVDGHGTHTSSTATGNA+AGASLS LAKGTARGGVPSAR
Sbjct: 181 IGARYFKLDGNTDPADILSPIDVDGHGTHTSSTATGNAVAGASLSGLAKGTARGGVPSAR 240

Query: 241 VAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGGFGNYSDDSISIGAFHAMKK 300
           VAMYKVCW STGCADMDILAAFDAAIHDGVDVISISIGGG FGNYSDDSISIGAFHAMKK
Sbjct: 241 VAMYKVCWASTGCADMDILAAFDAAIHDGVDVISISIGGGAFGNYSDDSISIGAFHAMKK 300

Query: 301 GIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFVSPLELGNGNNISGVAINTFNPK 360
           GIITVTSAGN GP  GS+VNHAPWIVTV ASAIDRKF+S LELGNG NISGV IN FNPK
Sbjct: 301 GIITVTSAGNDGPTPGSVVNHAPWIVTVGASAIDRKFISLLELGNGKNISGVGINIFNPK 360

Query: 361 EKMYPLVSGRDVARNSESKESASYCAEESLDPNKVKGNLVLCKFMSWGTDSVVKSLGGSG 420
           +KMYPLV G DVAR + ++ESAS+C E+SLDP+KVKG+LV C+ ++WG DSVV +LG +G
Sbjct: 361 KKMYPLVYGGDVARGAGNRESASHCEEDSLDPSKVKGSLVFCELITWGVDSVVSALGANG 420

Query: 421 VIIQSDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQFKATAPMVASF 480
            IIQS+++LDNA+IFMAPAT+VSS VG  I +YIKST+TP AVIY+TRQ KA APM ASF
Sbjct: 421 AIIQSNEYLDNANIFMAPATMVSSSVGAVIHSYIKSTRTPTAVIYKTRQLKAAAPMAASF 480

Query: 481 SSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTL----SLALPWPA 540
           SSRGPNPG+ RILKPDIAAPGVDILAGYTPLKSLTG +GDTQFSKFTL    S+A P  A
Sbjct: 481 SSRGPNPGTTRILKPDIAAPGVDILAGYTPLKSLTGQEGDTQFSKFTLMSGTSMACPHVA 540

Query: 541 LTSP---------PPPPMSSLSTPTARPISRRLNPDGEFAYGAGNLNPSRAKNPGLIYDL 600
             +           P  + S    TA  ISRRLNPDGEFAYGAGNLNPSRA NPGLIYDL
Sbjct: 541 AAAAYVKSFHPLWSPGAIRSALITTASQISRRLNPDGEFAYGAGNLNPSRAINPGLIYDL 600

Query: 601 NEMSYIQFLCREGYTGSSIAVLAGTKSINCSTLIPGLGHDSLNYPTIQLSLKSTQRPMTT 660
           NEMSYIQFLC EGYTG SI+VL GTKSINCSTLIPG GHDSLNYPT QL LKS +R M+T
Sbjct: 601 NEMSYIQFLCSEGYTGFSISVLTGTKSINCSTLIPGHGHDSLNYPTFQLRLKSPRRAMST 660

Query: 661 VFRRRVTNVGRAVSVYNATVRAPAGVEITVVPTTLSFSRLLQKRSFKIVVKASPLPSTKM 720
           VFRRRVTNV   VSVYNAT++AP GVEITV P+TLSFSRL QKRSFK+ VKASPL S KM
Sbjct: 661 VFRRRVTNVSHPVSVYNATIKAPPGVEITVTPSTLSFSRLQQKRSFKVAVKASPLASGKM 720

Query: 721 VSGSLAWVGARHVVRSPIVVYSP 731
           VSGS+AW+GARH VRSPIVVYSP
Sbjct: 721 VSGSVAWIGARHAVRSPIVVYSP 742

BLAST of Lag0041276 vs. TAIR 10
Match: AT4G00230.1 (xylem serine peptidase 1 )

HSP 1 Score: 840.1 bits (2169), Expect = 1.3e-243
Identity = 430/735 (58.50%), Postives = 546/735 (74.29%), Query Frame = 0

Query: 9   LLVFVLFFVVAFVAAVEIDEKKKHFIVFLENKPVLNEVDAVETHLNVLKSVKESHVEAKE 68
           +LV V+ ++    A+ E +  K  +I++L ++P  N  + ++TH+N+L S+  S  EAKE
Sbjct: 14  VLVMVVLWISPRYASAEDEHAKDFYIIYLGDRPD-NTEETIKTHINLLSSLNISQEEAKE 73

Query: 69  SMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHNVIPNKYRKLQTTRSWDFIGLPINARRN 128
             VYSYTK+FNAFAAKL+  EAK + + E+V +V  N+YRKL TT+SWDF+GLP+ A+R+
Sbjct: 74  RKVYSYTKAFNAFAAKLSPHEAKKMMEMEEVVSVSRNQYRKLHTTKSWDFVGLPLTAKRH 133

Query: 129 TKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCNNKLIGARYFKL 188
            K E D+++G+ DTGITP +ESF D G GPPP KWKGSC  + NFTGCNNK+IGA+YFK 
Sbjct: 134 LKAERDVIIGVLDTGITPDSESFLDHGLGPPPAKWKGSCGPYKNFTGCNNKIIGAKYFKH 193

Query: 189 DGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSDLAKGTARGGVPSARVAMYKVCW 248
           DG+    ++ SPID+DGHGTHTSST  G  +A ASL  +A GTARG VPSAR+AMYKVCW
Sbjct: 194 DGNVPAGEVRSPIDIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVPSARLAMYKVCW 253

Query: 249 ESTGCADMDILAAFDAAIHDGVDVISISIGGGGFGNYSDDSISIGAFHAMKKGIITVTSA 308
             +GCADMDILA F+AAIHDGV++ISISI GG   +YS DSIS+G+FHAM+KGI+TV SA
Sbjct: 254 ARSGCADMDILAGFEAAIHDGVEIISISI-GGPIADYSSDSISVGSFHAMRKGILTVASA 313

Query: 309 GNSGPVAGSIVNHAPWIVTVAASAIDRKFVSPLELGNGNNISGVAINTFNPKEKMYPLVS 368
           GN GP +G++ NH PWI+TVAAS IDR F S ++LGNG + SG+ I+ F+PK K YPLVS
Sbjct: 314 GNDGPSSGTVTNHEPWILTVAASGIDRTFKSKIDLGNGKSFSGMGISMFSPKAKSYPLVS 373

Query: 369 GRDVARNSESKESASYCAEESLDPNKVKGNLVLCKFMSWGTDSVVKSLGGSGVIIQSDQF 428
           G D A+N++ K  A YC  +SLD  KVKG +++C+    G +S +KS GG+G II SDQ+
Sbjct: 374 GVDAAKNTDDKYLARYCFSDSLDRKKVKGKVMVCRMGGGGVESTIKSYGGAGAIIVSDQY 433

Query: 429 LDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQFKATAPMVASFSSRGPNPG 488
           LDNA IFMAPAT V+S VGD I  YI ST++  AVI +TRQ    AP VASFSSRGPNPG
Sbjct: 434 LDNAQIFMAPATSVNSSVGDIIYRYINSTRSASAVIQKTRQVTIPAPFVASFSSRGPNPG 493

Query: 489 SNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTL----SLALPWPALTSP---- 548
           S R+LKPDIAAPG+DILA +T  +SLTGL GDTQFSKFT+    S+A P  A  +     
Sbjct: 494 SIRLLKPDIAAPGIDILAAFTLKRSLTGLDGDTQFSKFTILSGTSMACPHVAGVAAYVKS 553

Query: 549 -----PPPPMSSLSTPTARPISRRLNPDGEFAYGAGNLNPSRAKNPGLIYDLNEMSYIQF 608
                 P  + S    +A+PISRR+N D EFAYG G +NP RA +PGL+YD++++SY+QF
Sbjct: 554 FHPDWTPAAIKSAIITSAKPISRRVNKDAEFAYGGGQINPRRAASPGLVYDMDDISYVQF 613

Query: 609 LCREGYTGSSIAVLAGTKSINCSTLIPGLGHDSLNYPTIQLSLKSTQRPMTTVFRRRVTN 668
           LC EGY  +++A L GT+S++CS+++PGLGHDSLNYPTIQL+L+S +     VFRRRVTN
Sbjct: 614 LCGEGYNATTLAPLVGTRSVSCSSIVPGLGHDSLNYPTIQLTLRSAKTSTLAVFRRRVTN 673

Query: 669 VGRAVSVYNATVRAPAGVEITVVPTTLSFSRLLQKRSFKIVVKASPLPSTKMVSGSLAWV 728
           VG   SVY ATVRAP GVEITV P +LSFS+  QKRSFK+VVKA  +   K+VSG L W 
Sbjct: 674 VGPPSSVYTATVRAPKGVEITVEPQSLSFSKASQKRSFKVVVKAKQMTPGKIVSGLLVWK 733

Query: 729 GARHVVRSPIVVYSP 731
             RH VRSPIV+YSP
Sbjct: 734 SPRHSVRSPIVIYSP 746

BLAST of Lag0041276 vs. TAIR 10
Match: AT5G03620.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 561.2 bits (1445), Expect = 1.2e-159
Identity = 339/764 (44.37%), Postives = 473/764 (61.91%), Query Frame = 0

Query: 6   SSRLLVFVLFFVV---AFVAAVEIDE-KKKHFIVFLENKPVLNEVDAVETHLNVLKSVKE 65
           + R+ +F+L F +   A +AA E +  ++K +IV++      + V+A E H N+L +V  
Sbjct: 4   NQRVRLFMLCFCLVNNAVIAATEDENVERKPYIVYMGEATENSLVEAAENHHNLLMTVIG 63

Query: 66  SHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHNVIPNKYRKLQTTRSWDFIGL 125
              +A+E  +YSY K+ N F A+L   EA+ LS+ E V +V  N  R+L TTRSWDF+GL
Sbjct: 64  DESKARELKIYSYGKNINGFVARLFPHEAEKLSREEGVVSVFKNTQRQLHTTRSWDFLGL 123

Query: 126 -PINARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCNNKL 185
                +R+   ES+I+VG+ DTGI   + SF D G GPPP KWKG C    NFT CNNK+
Sbjct: 124 VESKYKRSVGIESNIIVGVLDTGIDVESPSFNDKGVGPPPAKWKGKCVTGNNFTRCNNKV 183

Query: 186 IGARYFKL------DGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSDLAKGTARG 245
           IGA+YF +      DG  D     +  D DGHGTHTSST  G +++ ASL  +A GTARG
Sbjct: 184 IGAKYFHIQSEGLPDGEGD-----TAADHDGHGTHTSSTIAGVSVSSASLFGIANGTARG 243

Query: 246 GVPSARVAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGGFGNYSDDSISIGA 305
           GVPSAR+A YKVCW+S GC DMD+LAAFD AI DGVD+ISISIGG     + +D I+IGA
Sbjct: 244 GVPSARIAAYKVCWDS-GCTDMDMLAAFDEAISDGVDIISISIGGASL-PFFEDPIAIGA 303

Query: 306 FHAMKKGIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFVSPLELGNGNNISGVAI 365
           FHAMK+GI+T  SAGN+GP   ++ N APW++TVAA+++DRKF + ++LGNG   SG+++
Sbjct: 304 FHAMKRGILTTCSAGNNGPGLFTVSNLAPWVMTVAANSLDRKFETVVKLGNGLTASGISL 363

Query: 366 NTFNPKEKMYPLVSGRDVAR-NSESKESASYCAEESLDPNKVKGNLVLCKF-------MS 425
           N FNP++KMYPL SG   +  ++      S C   +L  +KV G +V C+          
Sbjct: 364 NGFNPRKKMYPLTSGSLASNLSAGGYGEPSTCEPGTLGEDKVMGKVVYCEAGREEGGNGG 423

Query: 426 WGTDSVVKSLGGSGVIIQSDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYR 485
            G D VV+SL G+GVI+Q  +  D A   +   + V    G  I  YI STK P+AVI++
Sbjct: 424 QGQDHVVRSLKGAGVIVQLLEPTDMATSTLIAGSYVFFEDGTKITEYINSTKNPQAVIFK 483

Query: 486 TRQFKATAPMVASFSSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKF 545
           T+  K  AP ++SFS+RGP   S  ILKPDI+APG++ILA Y+ L S+TG   D + + F
Sbjct: 484 TKTTKMLAPSISSFSARGPQRISPNILKPDISAPGLNILAAYSKLASVTGYPDDNRRTLF 543

Query: 546 TL----SLALPWPALTSP---------PPPPMSSLSTPTARPISRRLNPDGEFAYGAGNL 605
           ++    S+A P  A  +           P  + S    TA P+  + N + E +YG+G +
Sbjct: 544 SIMSGTSMACPHAAAAAAYVKSFHPDWSPAAIKSALMTTATPMRIKGN-EAELSYGSGQI 603

Query: 606 NPSRAKNPGLIYDLNEMSYIQFLCREGYTGSSIAVLAG-------TKSINCSTLIPGLGH 665
           NP RA +PGL+YD+ E +Y++FLC+EGY  +SI +L G        K  NC  +  GLG 
Sbjct: 604 NPRRAIHPGLVYDITEDAYLRFLCKEGYNSTSIGLLTGDNSNNTTKKEYNCENIKRGLGS 663

Query: 666 DSLNYPTIQLSLKSTQRPMTTVFRRRVTNVGRAVSVYNATVRAPAGVEITVVPTTLSFSR 725
           D LNYP++   + ST+  ++ VF R VTNVG   S Y A V AP G+ + VVP  +SF R
Sbjct: 664 DGLNYPSLHKQVNSTEAKVSEVFYRTVTNVGYGPSTYVARVWAPKGLRVEVVPKVMSFER 723

Query: 726 LLQKRSFKIVVKASPLPSTK-MVSGSLAWVGAR-HVVRSPIVVY 729
             +KR+FK+V+      + K +VS S+ W  +R H+VRSPI+++
Sbjct: 724 PKEKRNFKVVIDGVWDETMKGIVSASVEWDDSRGHLVRSPILLF 759

BLAST of Lag0041276 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 496.1 bits (1276), Expect = 4.7e-140
Identity = 294/695 (42.30%), Postives = 422/695 (60.72%), Query Frame = 0

Query: 52  HLNVLKSVKESHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHNVIPNKYRKLQ 111
           HL++L+ +  + + A   +V SY +SFN FAA L++ E++ L   ++V +V P+K  +L 
Sbjct: 15  HLSILQKLVGT-IAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELT 74

Query: 112 TTRSWDFIGLPINARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFA 171
           TTRSWDF+G    ARR + +ESD++VG+ D+GI P +ESF D+GFGPPPKKWKGSC    
Sbjct: 75  TTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGL 134

Query: 172 NFTGCNNKLIGARYFKLDGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSDLAKGT 231
            F  CNNKLIGAR++            S  D +GHGTHT+STA GNA+  AS   LA+GT
Sbjct: 135 KF-ACNNKLIGARFYNKFAD-------SARDEEGHGTHTASTAAGNAVQAASFYGLAQGT 194

Query: 232 ARGGVPSARVAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGGFGNYSDDSIS 291
           ARGGVPSAR+A YKVC+    C D+DILAAFD AI DGVDVISISI      N  + S++
Sbjct: 195 ARGGVPSARIAAYKVCFNR--CNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVA 254

Query: 292 IGAFHAMKKGIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFVSPLELGNGNNISG 351
           IG+FHAM +GIIT  SAGN+GP  GS+ N +PW++TVAAS  DR+F+  + LGNG  ++G
Sbjct: 255 IGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTG 314

Query: 352 VAINTFNPKEKMYPLVSGRDVARNSESKESASYCAEESLDPNKVKGNLVLCKFMSWGTDS 411
           +++NTFN     +P+V G++V+RN  S+  A YC+   +D   VKG +VLC       ++
Sbjct: 315 ISVNTFNLNGTKFPIVYGQNVSRNC-SQAQAGYCSSGCVDSELVKGKIVLCDDFLGYREA 374

Query: 412 VVKSLGGSGVIIQSDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQF- 471
            +   G  GVI+Q+    D+A +   PA+ +      +I +YI+S + P+A I RT +  
Sbjct: 375 YL--AGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIV 434

Query: 472 KATAPMVASFSSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGL-----KGDTQFSK 531
              AP V SFSSRGP+     +LKPD++APG++ILA ++P+ S +       K   ++S 
Sbjct: 435 DREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSV 494

Query: 532 FT-LSLALPWPALTSP---------PPPPMSSLSTPTARPISRRLNPDGEFAYGAGNLNP 591
            +  S+A P  A  +           P  + S    TA P++ + NP+ EFAYG+G +NP
Sbjct: 495 MSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINP 554

Query: 592 SRAKNPGLIYDLNEMSYIQFLCREGYTGSSIAVLAGTKSINCSTLIPGLGHDSLNYPTIQ 651
           ++A +PGL+Y++    Y++ LC EG+  +++   +G +++ CS          LNYPT+ 
Sbjct: 555 TKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSG-QNVTCSERTE---VKDLNYPTMT 614

Query: 652 LSLKSTQRPMTTVFRRRVTNVGRAVSVYNAT-VRAPAGVEITVVPTTLSFSRLLQKRSFK 711
             + S   P    F+R VTNVG   S Y A+ V     ++I++ P  L F  L +K+SF 
Sbjct: 615 TFVSSLD-PFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFV 674

Query: 712 IVVKASPLPSTKMVSGSLAWVGARHVVRSPIVVYS 730
           + +    L     VS S+ W    H VRSPIV YS
Sbjct: 675 VTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYS 690

BLAST of Lag0041276 vs. TAIR 10
Match: AT5G59120.1 (subtilase 4.13 )

HSP 1 Score: 493.0 bits (1268), Expect = 4.0e-139
Identity = 311/749 (41.52%), Postives = 453/749 (60.48%), Query Frame = 0

Query: 2   SISKSSRLLVFVLFFVVAFVAAVEIDEKKKHFIVFLENKPVLNEVDAVETHLNVLKSVK- 61
           +++ SS LL  +L   ++ V+AV  D  K+ +IV++ +     +      H+N+L+ V  
Sbjct: 3   TLAASSSLLSCLLVLFLSSVSAVTDD--KQVYIVYMGSLSSRADYTPTSDHMNILQEVTG 62

Query: 62  ESHVEAKESMVYSYTKSFNAFAAKLTEEEAKTLSKREDVHNVIPNKYRKLQTTRSWDFIG 121
           ES +E +  +V SY +SFN FAA+LTE E + ++K   V +V PNK  +LQTT SWDF+G
Sbjct: 63  ESSIEGR--LVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMG 122

Query: 122 LP--INARRNTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCNN 181
           L   I  +RN   ESD ++G+ D+GITP ++SF D GFGPPP+KWKG C    NFT CNN
Sbjct: 123 LKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFT-CNN 182

Query: 182 KLIGARYFKLDGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSDLAKGTARGGVPS 241
           KLIGAR +  +G+          D+DGHGTHT+STA GNA+  AS   +  GT RGGVP+
Sbjct: 183 KLIGARDYTSEGTR---------DMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPA 242

Query: 242 ARVAMYKVCWESTGCADMDILAAFDAAIHDGVDVISISIGGGGFGNYSDDSISIGAFHAM 301
           +RVA YKVC   TGC+   +L+AFD AI DGVD+I+ISIG      + +D I+IGAFHAM
Sbjct: 243 SRVAAYKVC-TPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAM 302

Query: 302 KKGIITVTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFVSPLELGNGNNISGVAINTFN 361
            KG++TV SAGNSGP   S+   APWI+TVAAS  +R FV+ + LGNG  + G ++N + 
Sbjct: 303 AKGVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYE 362

Query: 362 PKEKMYPLVSGRDVARNSESKESASYCAEESLDPNKVKGNLVLCKFMSWGTDSVVKSLGG 421
            K K YPLV G+  A ++   ESA  C    +D ++VKG +++C     G   +V+S+G 
Sbjct: 363 MKGKDYPLVYGKSAASSACDAESAGLCELSCVDKSRVKGKILVCG--GPGGLKIVESVGA 422

Query: 422 SGVIIQSDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQ-FKATAPMV 481
            G+I ++ +  D A I   PA  + +   +++ +Y++ST +P+A++ +T   F  T+P++
Sbjct: 423 VGLIYRTPK-PDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVI 482

Query: 482 ASFSSRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTL----SLALP 541
           ASFSSRGPN  +  ILKPDI APGV+ILA Y+P    +  + DT+  K+++    S++ P
Sbjct: 483 ASFSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPS--QDDTRHVKYSVLSGTSMSCP 542

Query: 542 WPALTSP---------PPPPMSSLSTPTARPISRRLN--PDGEFAYGAGNLNPSRAKNPG 601
             A  +           P  + S    TA P++         EFAYG+G+++P  A NPG
Sbjct: 543 HVAGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPG 602

Query: 602 LIYDLNEMSYIQFLCREGYTGSSIAVLAGTKSINCSTLIPGLGHDSLNYPTIQLSLKSTQ 661
           L+Y+L++  +I FLC   YT   + V++G +++ CS     L   +LNYP++   L  + 
Sbjct: 603 LVYELDKSDHIAFLCGMNYTSQVLKVISG-ETVTCSEAKKILPR-NLNYPSMSAKLSGSG 662

Query: 662 RPMTTVFRRRVTNVGRAVSVYNATVRAPAG--VEITVVPTTLSFSRLLQKRSFKIVVKAS 721
              T  F R +TNVG   S Y + V A  G  +++ + P+ LSF  + +K+SF + V  S
Sbjct: 663 TTFTVTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGS 722

Query: 722 PLPSTKMVSGSLAWVGARHVVRSPIVVYS 730
            L S    S +L W    H VRSPIVVY+
Sbjct: 723 NLDSEVPSSANLIWSDGTHNVRSPIVVYT 729

BLAST of Lag0041276 vs. TAIR 10
Match: AT3G46850.1 (Subtilase family protein )

HSP 1 Score: 483.0 bits (1242), Expect = 4.2e-136
Identity = 302/742 (40.70%), Postives = 443/742 (59.70%), Query Frame = 0

Query: 11  VFVLFFVVAFVAAVEIDEKKKHFIVFLENKPVLNEVDAVETHLNVLKSVK-ESHVEAKES 70
           +F L  VV+F +A + D+ K+ +IV++   P   +   +  H ++L+ V  ES ++  + 
Sbjct: 13  IFAL-LVVSFASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGESSIQ--DR 72

Query: 71  MVYSYTKSFNAFAAKLTEEEAKTLSKREDVHNVIPNKYRKLQTTRSWDFIGLPINAR--R 130
           +V +Y +SFN FAA+LTE E + L+  ++V +V P+K   LQTT SW+F+GL    R  R
Sbjct: 73  LVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRTKR 132

Query: 131 NTKQESDIVVGLFDTGITPTAESFKDDGFGPPPKKWKGSCHHFANFTGCNNKLIGARYF- 190
           N   ESD ++G+ D+GI P ++SF   GFGPPPKKWKG C    NFT CNNKLIGARY+ 
Sbjct: 133 NPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFT-CNNKLIGARYYT 192

Query: 191 -KLDGSSDPTDILSPIDVDGHGTHTSSTATGNAIAGASLSDLAKGTARGGVPSARVAMYK 250
            KL+G  +     S  D  GHG+HT+S A GNA+   S   L  GT RGGVP+AR+A+YK
Sbjct: 193 PKLEGFPE-----SARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVYK 252

Query: 251 VCWEST-GCADMDILAAFDAAIHDGVDVISISIGGGGFGNYSDDSISIGAFHAMKKGIIT 310
           VC      C    ILAAFD AI D VD+I++S+G    G + +D+++IGAFHAM KGI+T
Sbjct: 253 VCDPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGILT 312

Query: 311 VTSAGNSGPVAGSIVNHAPWIVTVAASAIDRKFVSPLELGNGNNISGVAINTFNPKEKMY 370
           V  AGN+GP   +IV+ APW+ TVAAS ++R F++ + LGNG  I G ++N+F+   K Y
Sbjct: 313 VNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKKY 372

Query: 371 PLVSGRDVARNSESKESASYCAEESLDPNKVKGNLVLCKFMSWGTDSVVKSLGGSGVIIQ 430
           PLV G+  +   ++  SA +C+   LD  +VKG +VLC   +       +++G    I++
Sbjct: 373 PLVYGKSASSRCDA-SSAGFCSPGCLDSKRVKGKIVLCD--TQRNPGEAQAMGAVASIVR 432

Query: 431 SDQFLDNADIFMAPATVVSSLVGDTIANYIKSTKTPKAVIYRTRQ-FKATAPMVASFSSR 490
            + + D A +F  P +V+S    + + +Y+ STK PKA + ++   F   AP+VAS+SSR
Sbjct: 433 -NPYEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSR 492

Query: 491 GPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTL----SLALPWPALTS 550
           GPNP  + ILKPDI APG +ILA Y+P   +   + DT+  K+T+    S++ P  A  +
Sbjct: 493 GPNPLIHDILKPDITAPGSEILAAYSPY--VPPSESDTRHVKYTVISGTSMSCPHVAGVA 552

Query: 551 P---------PPPPMSSLSTPTARPISRRLNPD---GEFAYGAGNLNPSRAKNPGLIYDL 610
                      P  + S    TA P++   +P     EFAYGAG+++P  A +PGL+Y+ 
Sbjct: 553 AYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEA 612

Query: 611 NEMSYIQFLCREGYTGSSIAVLAGTKSINCSTLIPGLGHDSLNYPTIQLSLKSTQRPMTT 670
           N+  +I FLC   YTG  + +++G  S +C+         +LNYP++   +  T +P   
Sbjct: 613 NKSDHITFLCGFNYTGKKLRLISGDSS-SCTKEQTKSLTRNLNYPSMSAQVSGT-KPFKV 672

Query: 671 VFRRRVTNVGRAVSVYNATVRAPAGVEITVVPTTLSFSRLLQKRSFKIVVKASPLPSTKM 730
            FRR VTNVGR  + Y A V   + +++ VVP  LS   L +K+SF + V  +   +  +
Sbjct: 673 TFRRTVTNVGRPNATYKAKV-VGSKLKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAENL 732