Lag0041274 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0041274 vs. NCBI nr
Match: XP_038891596.1 (subtilisin-like protease SBT4.14 [Benincasa hispida])

HSP 1 Score: 910.2 bits (2351), Expect = 8.8e-261
Identity = 450/559 (80.50%), Postives = 507/559 (90.70%), Query Frame = 0

Query: 6    SKIFQARRQPRSLDILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPSARVA 65
            ++ F+    P  LDILSPVD++GHGTHT+S A GN + GA+LSGLA GTARGGVPSARVA
Sbjct: 534  ARYFKLDGLPDPLDILSPVDVNGHGTHTASTAAGNVIAGANLSGLAQGTARGGVPSARVA 593

Query: 66   MYKVCWSGTGCSDMDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAMKKGI 125
            MYKVCW+ TGCSDMDLLAAFDAAI+DGVDVISISIAG GFGNYTED ++IGAF AMKKGI
Sbjct: 594  MYKVCWTSTGCSDMDLLAAFDAAIRDGVDVISISIAGIGFGNYTEDPISIGAFQAMKKGI 653

Query: 126  ITVTAAGNTGPTAGSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISGVGINLFNPNQK 185
            ITV AAGN GP+AG+VVNHAPWI+TVAAS IDR+F+SP +LGNGKNISGVGINLFNP QK
Sbjct: 654  ITVAAAGNNGPSAGTVVNHAPWIVTVAASSIDRRFISPLQLGNGKNISGVGINLFNPQQK 713

Query: 186  MYPLVSGGDVAMNPQSKDSASFCVDGTLDPSKVKGKLVFCKLITWGADSVVKSIGAYGTI 245
            MY LVSGGDVA + +SKDSA FCV+G+LDP KVK  +VFCKL+TWGADSVVKSIGAYG I
Sbjct: 714  MYKLVSGGDVAKDIESKDSAMFCVEGSLDPIKVKDSIVFCKLMTWGADSVVKSIGAYGAI 773

Query: 246  IQSDQFLPNTDLFMAPASLVSSLVGETIDNYIHSTRAPTAVIYKSRQLKSAAPIVAFFSS 305
            +QSDQF+ NTD+FMAP++LVSS VG TID+YIHSTR PTAVIYK+RQL++AAP VA FSS
Sbjct: 774  LQSDQFIDNTDIFMAPSTLVSSFVGLTIDSYIHSTRTPTAVIYKTRQLRAAAPFVAAFSS 833

Query: 306  RGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAA 365
            RGPNPGS RILKPDIAAPGV+ILAGYTPLKSLTGLKGD+QFSKFTLMSGTSMACPHVAAA
Sbjct: 834  RGPNPGSTRILKPDIAAPGVNILAGYTPLKSLTGLKGDSQFSKFTLMSGTSMACPHVAAA 893

Query: 366  AAYVKSFHPLWSPAAIRSALLTTASPVSRRLNPDGEFSYGAGNLSPSRARNPGLIYDLNE 425
            A YVKSFHPLWSPAAIRSALLTTA+P+SRR+NP+GEF YGAGNL+P++A+NPGLIYDL++
Sbjct: 894  ATYVKSFHPLWSPAAIRSALLTTATPISRRMNPNGEFGYGAGNLNPAKAKNPGLIYDLDD 953

Query: 426  MSYIQFLCSEGYTGSSISILTGTKSIDCST-LRGQGYDSLNYPTFQLSLKPTRQPTTAVF 485
            MSYIQFLCSEGYTGSSI+ILTGTKS+DCST + GQGYDSLNYPTFQL+LK T+QPTTAVF
Sbjct: 954  MSYIQFLCSEGYTGSSIAILTGTKSLDCSTIIPGQGYDSLNYPTFQLTLKSTQQPTTAVF 1013

Query: 486  WRQVTNVGRPVSVYNATVRAPPGVEITVVPATLSFSQLLQKQNFKVIVKASPLPSAKMVS 545
            WR+VTNVGRPVS YNAT+RAPPGVEI VVP TLSFSQLLQKQ FKV+VK+ PLPS  MVS
Sbjct: 1014 WREVTNVGRPVSTYNATIRAPPGVEIMVVPTTLSFSQLLQKQRFKVVVKSGPLPSTNMVS 1073

Query: 546  GSLTWAAARYVVRSPIVIY 564
            G ++W   RYVVRSPIV+Y
Sbjct: 1074 GWISWVDVRYVVRSPIVVY 1092

BLAST of Lag0041274 vs. NCBI nr
Match: KAA0056493.1 (subtilisin-like protease SBT4.14 [Cucumis melo var. makuwa] >TYK29030.1 subtilisin-like protease SBT4.14 [Cucumis melo var. makuwa])

HSP 1 Score: 907.1 bits (2343), Expect = 7.4e-260
Identity = 456/547 (83.36%), Postives = 498/547 (91.04%), Query Frame = 0

Query: 18  LDILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPSARVAMYKVCWSGTGCS 77
           LDILSP+DI+GHGTHTSS ATGN + GA+LSGLA GTARGGVPSARVAMYKVCW+ TGCS
Sbjct: 55  LDILSPIDINGHGTHTSSTATGNVITGANLSGLAQGTARGGVPSARVAMYKVCWTSTGCS 114

Query: 78  DMDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAMKKGIITVTAAGNTGPT 137
           DMDLLAAFDAAIQDGVDVISISIAG G+GNYTED ++IGAFHAMKKGIITVTAAGN GP+
Sbjct: 115 DMDLLAAFDAAIQDGVDVISISIAGIGYGNYTEDPISIGAFHAMKKGIITVTAAGNNGPS 174

Query: 138 AGSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISGVGINLFNPNQKMYPLVSGGDVAM 197
           AG+VVNHAPWILTVAAS IDR F+SP ELGNGKNISGVGINLFNP +KMY LVSG DVA 
Sbjct: 175 AGTVVNHAPWILTVAASSIDRTFISPVELGNGKNISGVGINLFNPEKKMYKLVSGEDVAK 234

Query: 198 NPQSKDSASFCVDGTLDPSKVKGKLVFCKLITWGADSVVKSIGAYGTIIQSDQFLPNTDL 257
           N +SKD A++CV+ +LDPSKVK  +VFCKL+TWGADS VKSIGA G I+QSDQFL NTD+
Sbjct: 235 NIESKDDATYCVENSLDPSKVKDSIVFCKLLTWGADSAVKSIGAAGAILQSDQFLDNTDI 294

Query: 258 FMAPASLVSSLVGETIDNYIHSTRAPTAVIYKSRQLKSAAPIVAFFSSRGPNPGSNRILK 317
           FMAP++LVSS VG TID+YIHS+R PTAVIYK+RQ   AAPIVA FSSRGPNPGS RILK
Sbjct: 295 FMAPSTLVSSFVGATIDSYIHSSRTPTAVIYKTRQHIGAAPIVAPFSSRGPNPGSTRILK 354

Query: 318 PDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWS 377
           PDIAAPGV+ILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWS
Sbjct: 355 PDIAAPGVNILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWS 414

Query: 378 PAAIRSALLTTASPVSRRLNPDGEFSYGAGNLSPSRARNPGLIYDLNEMSYIQFLCSEGY 437
           PAAIRSALLTTA P+SRR NPDGEF YGAGNL+P +A+NPGLIYDLNEMSYIQFLCSEGY
Sbjct: 415 PAAIRSALLTTAKPISRRGNPDGEFGYGAGNLNPGKAKNPGLIYDLNEMSYIQFLCSEGY 474

Query: 438 TGSSISILTGTKSIDCST-LRGQGYDSLNYPTFQLSLKPTRQPTTAVFWRQVTNVGRPVS 497
           +GSSISILTGTKSI+C+T +RGQGYDSLNYPTFQL L+ TRQPTTAVFWR+VTNVG PVS
Sbjct: 475 SGSSISILTGTKSINCATIIRGQGYDSLNYPTFQLRLQSTRQPTTAVFWREVTNVGTPVS 534

Query: 498 VYNATVRAPPGVEITVVPATLSFSQLLQKQNFKVIVKASPLPSAKMVSGSLTWAAARYVV 557
           VYN TV APPGVEITV PATLSFS LLQK+ FKV+VKA+PLPS KMVSGS+TW   RYVV
Sbjct: 535 VYNTTVWAPPGVEITVEPATLSFSYLLQKERFKVVVKANPLPSNKMVSGSITWFNPRYVV 594

Query: 558 RSPIVIY 564
           RSPIV+Y
Sbjct: 595 RSPIVVY 601

BLAST of Lag0041274 vs. NCBI nr
Match: XP_008454861.1 (PREDICTED: subtilisin-like protease SBT4.14 [Cucumis melo])

HSP 1 Score: 907.1 bits (2343), Expect = 7.4e-260
Identity = 456/547 (83.36%), Postives = 498/547 (91.04%), Query Frame = 0

Query: 18  LDILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPSARVAMYKVCWSGTGCS 77
           LDILSP+DI+GHGTHTSS ATGN + GA+LSGLA GTARGGVPSARVAMYKVCW+ TGCS
Sbjct: 227 LDILSPIDINGHGTHTSSTATGNVITGANLSGLAQGTARGGVPSARVAMYKVCWTSTGCS 286

Query: 78  DMDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAMKKGIITVTAAGNTGPT 137
           DMDLLAAFDAAIQDGVDVISISIAG G+GNYTED ++IGAFHAMKKGIITVTAAGN GP+
Sbjct: 287 DMDLLAAFDAAIQDGVDVISISIAGIGYGNYTEDPISIGAFHAMKKGIITVTAAGNNGPS 346

Query: 138 AGSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISGVGINLFNPNQKMYPLVSGGDVAM 197
           AG+VVNHAPWILTVAAS IDR F+SP ELGNGKNISGVGINLFNP +KMY LVSG DVA 
Sbjct: 347 AGTVVNHAPWILTVAASSIDRTFISPVELGNGKNISGVGINLFNPEKKMYKLVSGEDVAK 406

Query: 198 NPQSKDSASFCVDGTLDPSKVKGKLVFCKLITWGADSVVKSIGAYGTIIQSDQFLPNTDL 257
           N +SKD A++CV+ +LDPSKVK  +VFCKL+TWGADS VKSIGA G I+QSDQFL NTD+
Sbjct: 407 NIESKDDATYCVENSLDPSKVKDSIVFCKLLTWGADSAVKSIGAAGAILQSDQFLDNTDI 466

Query: 258 FMAPASLVSSLVGETIDNYIHSTRAPTAVIYKSRQLKSAAPIVAFFSSRGPNPGSNRILK 317
           FMAP++LVSS VG TID+YIHS+R PTAVIYK+RQ   AAPIVA FSSRGPNPGS RILK
Sbjct: 467 FMAPSTLVSSFVGATIDSYIHSSRTPTAVIYKTRQHIGAAPIVAPFSSRGPNPGSTRILK 526

Query: 318 PDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWS 377
           PDIAAPGV+ILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWS
Sbjct: 527 PDIAAPGVNILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWS 586

Query: 378 PAAIRSALLTTASPVSRRLNPDGEFSYGAGNLSPSRARNPGLIYDLNEMSYIQFLCSEGY 437
           PAAIRSALLTTA P+SRR NPDGEF YGAGNL+P +A+NPGLIYDLNEMSYIQFLCSEGY
Sbjct: 587 PAAIRSALLTTAKPISRRGNPDGEFGYGAGNLNPGKAKNPGLIYDLNEMSYIQFLCSEGY 646

Query: 438 TGSSISILTGTKSIDCST-LRGQGYDSLNYPTFQLSLKPTRQPTTAVFWRQVTNVGRPVS 497
           +GSSISILTGTKSI+C+T +RGQGYDSLNYPTFQL L+ TRQPTTAVFWR+VTNVG PVS
Sbjct: 647 SGSSISILTGTKSINCATIIRGQGYDSLNYPTFQLRLQSTRQPTTAVFWREVTNVGTPVS 706

Query: 498 VYNATVRAPPGVEITVVPATLSFSQLLQKQNFKVIVKASPLPSAKMVSGSLTWAAARYVV 557
           VYN TV APPGVEITV PATLSFS LLQK+ FKV+VKA+PLPS KMVSGS+TW   RYVV
Sbjct: 707 VYNTTVWAPPGVEITVEPATLSFSYLLQKERFKVVVKANPLPSNKMVSGSITWFNPRYVV 766

Query: 558 RSPIVIY 564
           RSPIV+Y
Sbjct: 767 RSPIVVY 773

BLAST of Lag0041274 vs. NCBI nr
Match: XP_011654432.1 (subtilisin-like protease SBT4.14 [Cucumis sativus] >KGN49552.1 hypothetical protein Csa_018364 [Cucumis sativus])

HSP 1 Score: 898.3 bits (2320), Expect = 3.4e-257
Identity = 448/559 (80.14%), Postives = 503/559 (89.98%), Query Frame = 0

Query: 6   SKIFQARRQPRSLDILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPSARVA 65
           ++ F+    P   DILSPVD  GHGTHTSS ATGNA+ GASLSGLA GTARGGVPSARVA
Sbjct: 183 ARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGVPSARVA 242

Query: 66  MYKVCWSGTGCSDMDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAMKKGI 125
           MYKVCW+ +GCSDMD+LAAFDAAIQDGVDVISISI G GF NY++D+++IGAFHAMKKGI
Sbjct: 243 MYKVCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHAMKKGI 302

Query: 126 ITVTAAGNTGPTAGSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISGVGINLFNPNQK 185
           ITVT+AGN GPTAGSVVNHAPWI+TVAAS IDRKF+SP ELGNGKNISGVGIN+FNP QK
Sbjct: 303 ITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIFNPKQK 362

Query: 186 MYPLVSGGDVAMNPQSKDSASFCVDGTLDPSKVKGKLVFCKLITWGADSVVKSIGAYGTI 245
           MYPLVSGGDVA N +SKD+ASFC++GTLDP+KVKG LVFCKL+TWGADSV+KSIGA G I
Sbjct: 363 MYPLVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSIGANGVI 422

Query: 246 IQSDQFLPNTDLFMAPASLVSSLVGETIDNYIHSTRAPTAVIYKSRQLKSAAPIVAFFSS 305
           IQSD+FL N D+FMAPA++VSSLVG  I  YI STR PTAVIYK++QLK+ AP+VA FSS
Sbjct: 423 IQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQLKAKAPMVASFSS 482

Query: 306 RGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAA 365
           RGPNPGS+RILKPDIAAPGVDILA YTPLKSLTG KGDTQ+SKFTLMSGTSMACPHVAAA
Sbjct: 483 RGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMACPHVAAA 542

Query: 366 AAYVKSFHPLWSPAAIRSALLTTASPVSRRLNPDGEFSYGAGNLSPSRARNPGLIYDLNE 425
           AAYVKSFHPLWSPAAIRSALLTTA+P+SRRLNP+GEF+YGAGNL+PSRA +PGLIYDLNE
Sbjct: 543 AAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGNLNPSRAISPGLIYDLNE 602

Query: 426 MSYIQFLCSEGYTGSSISILTGTKSIDCSTL-RGQGYDSLNYPTFQLSLKPTRQPTTAVF 485
           +SYIQFLCSEGYTGSSI++L+GTKSI+CS L  GQG+DSLNYPTFQLSLK T QP T  F
Sbjct: 603 ISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQPMTTTF 662

Query: 486 WRQVTNVGRPVSVYNATVRAPPGVEITVVPATLSFSQLLQKQNFKVIVKASPLPSAKMVS 545
            R+VTNVG P+SVYNAT+ APPGV ITV P TLSFS+LLQK++FKV+VKASPLPSAKMVS
Sbjct: 663 RRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLPSAKMVS 722

Query: 546 GSLTWAAARYVVRSPIVIY 564
           GSL W  A++VVRSPIV+Y
Sbjct: 723 GSLAWVGAQHVVRSPIVVY 741

BLAST of Lag0041274 vs. NCBI nr
Match: XP_038892956.1 (subtilisin-like protease SBT4.14 [Benincasa hispida])

HSP 1 Score: 890.2 bits (2299), Expect = 9.4e-255
Identity = 443/559 (79.25%), Postives = 499/559 (89.27%), Query Frame = 0

Query: 6   SKIFQARRQPRSLDILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPSARVA 65
           ++ F+    P   DILSPVD  GHGTHTSS ATGNAV GASLSGLA GTARGGVPSARV+
Sbjct: 183 ARYFKLDGSPDPADILSPVDTDGHGTHTSSTATGNAVAGASLSGLAEGTARGGVPSARVS 242

Query: 66  MYKVCWSGTGCSDMDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAMKKGI 125
           MYKVCW+ +GCSDMD+LAAFDAAI DGVDVISISI G GF NY+ED+++IGAFHAMKKGI
Sbjct: 243 MYKVCWASSGCSDMDILAAFDAAIHDGVDVISISIGGGGFNNYSEDSISIGAFHAMKKGI 302

Query: 126 ITVTAAGNTGPTAGSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISGVGINLFNPNQK 185
           ITVT+AGN+GP AGSVVNHAPWI+TVAAS IDRKF+SP ELGNGKNISGVGIN+FNP QK
Sbjct: 303 ITVTSAGNSGPLAGSVVNHAPWIVTVAASAIDRKFISPLELGNGKNISGVGINIFNPKQK 362

Query: 186 MYPLVSGGDVAMNPQSKDSASFCVDGTLDPSKVKGKLVFCKLITWGADSVVKSIGAYGTI 245
           MYPLVSGGDVA N +SKDSAS+C + +LDP+KVKG LVFCKL+TWGADSV+KS+GA G I
Sbjct: 363 MYPLVSGGDVARNSESKDSASYCEEDSLDPNKVKGNLVFCKLMTWGADSVIKSLGANGVI 422

Query: 246 IQSDQFLPNTDLFMAPASLVSSLVGETIDNYIHSTRAPTAVIYKSRQLKSAAPIVAFFSS 305
           IQSDQFL N D+FMAPA++VSS +G  I  YI STR PTAVIYK++QLK+ AP+VA FSS
Sbjct: 423 IQSDQFLDNADIFMAPATMVSSSLGTIIHTYIKSTRTPTAVIYKTKQLKAKAPMVASFSS 482

Query: 306 RGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAA 365
           RGPNPGSNRILKPDIAAPGVDILA YTPLKSLTGLKGDTQ+SKFTLMSGTSMACPHVAAA
Sbjct: 483 RGPNPGSNRILKPDIAAPGVDILASYTPLKSLTGLKGDTQYSKFTLMSGTSMACPHVAAA 542

Query: 366 AAYVKSFHPLWSPAAIRSALLTTASPVSRRLNPDGEFSYGAGNLSPSRARNPGLIYDLNE 425
           AAYVKSFHP WSPAAIRSAL+TTA P+SRRLNPDGEF+YGAGNL+PSRA +PGLIYD+NE
Sbjct: 543 AAYVKSFHPHWSPAAIRSALITTARPISRRLNPDGEFAYGAGNLNPSRAISPGLIYDVNE 602

Query: 426 MSYIQFLCSEGYTGSSISILTGTKSIDCSTL-RGQGYDSLNYPTFQLSLKPTRQPTTAVF 485
           +SY+QFLCSEGYTGSSI++L GTKSI+CS L  GQG+DSLNYPTFQLSLK TRQPTT  F
Sbjct: 603 ISYVQFLCSEGYTGSSIAVLAGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTRQPTTTTF 662

Query: 486 WRQVTNVGRPVSVYNATVRAPPGVEITVVPATLSFSQLLQKQNFKVIVKASPLPSAKMVS 545
            R+VTNV  PVSV+NAT++APPGVEITV P TLSFS+LLQK++FKV+VKASPLPS KMVS
Sbjct: 663 RRRVTNVAHPVSVFNATIKAPPGVEITVTPTTLSFSRLLQKRSFKVVVKASPLPSTKMVS 722

Query: 546 GSLTWAAARYVVRSPIVIY 564
           GS+ W  AR+VVRSPIV+Y
Sbjct: 723 GSIAWIGARHVVRSPIVVY 741

BLAST of Lag0041274 vs. ExPASy Swiss-Prot
Match: Q9LLL8 (Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana OX=3702 GN=SBT4.14 PE=2 SV=1)

HSP 1 Score: 664.1 bits (1712), Expect = 1.4e-189
Identity = 330/546 (60.44%), Postives = 424/546 (77.66%), Query Frame = 0

Query: 19  DILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPSARVAMYKVCWSGTGCSD 78
           ++ SP+DI GHGTHTSS   G  V  ASL G+A GTARG VPSAR+AMYKVCW+ +GC+D
Sbjct: 200 EVRSPIDIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVPSARLAMYKVCWARSGCAD 259

Query: 79  MDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAMKKGIITVTAAGNTGPTA 138
           MD+LA F+AAI DGV++ISISI G    +Y+ D++++G+FHAM+KGI+TV +AGN GP++
Sbjct: 260 MDILAGFEAAIHDGVEIISISI-GGPIADYSSDSISVGSFHAMRKGILTVASAGNDGPSS 319

Query: 139 GSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISGVGINLFNPNQKMYPLVSGGDVAMN 198
           G+V NH PWILTVAASGIDR F S  +LGNGK+ SG+GI++F+P  K YPLVSG D A N
Sbjct: 320 GTVTNHEPWILTVAASGIDRTFKSKIDLGNGKSFSGMGISMFSPKAKSYPLVSGVDAAKN 379

Query: 199 PQSKDSASFCVDGTLDPSKVKGKLVFCKLITWGADSVVKSIGAYGTIIQSDQFLPNTDLF 258
              K  A +C   +LD  KVKGK++ C++   G +S +KS G  G II SDQ+L N  +F
Sbjct: 380 TDDKYLARYCFSDSLDRKKVKGKVMVCRMGGGGVESTIKSYGGAGAIIVSDQYLDNAQIF 439

Query: 259 MAPASLVSSLVGETIDNYIHSTRAPTAVIYKSRQLKSAAPIVAFFSSRGPNPGSNRILKP 318
           MAPA+ V+S VG+ I  YI+STR+ +AVI K+RQ+   AP VA FSSRGPNPGS R+LKP
Sbjct: 440 MAPATSVNSSVGDIIYRYINSTRSASAVIQKTRQVTIPAPFVASFSSRGPNPGSIRLLKP 499

Query: 319 DIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSP 378
           DIAAPG+DILA +T  +SLTGL GDTQFSKFT++SGTSMACPHVA  AAYVKSFHP W+P
Sbjct: 500 DIAAPGIDILAAFTLKRSLTGLDGDTQFSKFTILSGTSMACPHVAGVAAYVKSFHPDWTP 559

Query: 379 AAIRSALLTTASPVSRRLNPDGEFSYGAGNLSPSRARNPGLIYDLNEMSYIQFLCSEGYT 438
           AAI+SA++T+A P+SRR+N D EF+YG G ++P RA +PGL+YD++++SY+QFLC EGY 
Sbjct: 560 AAIKSAIITSAKPISRRVNKDAEFAYGGGQINPRRAASPGLVYDMDDISYVQFLCGEGYN 619

Query: 439 GSSISILTGTKSIDCSTL-RGQGYDSLNYPTFQLSLKPTRQPTTAVFWRQVTNVGRPVSV 498
            ++++ L GT+S+ CS++  G G+DSLNYPT QL+L+  +  T AVF R+VTNVG P SV
Sbjct: 620 ATTLAPLVGTRSVSCSSIVPGLGHDSLNYPTIQLTLRSAKTSTLAVFRRRVTNVGPPSSV 679

Query: 499 YNATVRAPPGVEITVVPATLSFSQLLQKQNFKVIVKASPLPSAKMVSGSLTWAAARYVVR 558
           Y ATVRAP GVEITV P +LSFS+  QK++FKV+VKA  +   K+VSG L W + R+ VR
Sbjct: 680 YTATVRAPKGVEITVEPQSLSFSKASQKRSFKVVVKAKQMTPGKIVSGLLVWKSPRHSVR 739

Query: 559 SPIVIY 564
           SPIVIY
Sbjct: 740 SPIVIY 744

BLAST of Lag0041274 vs. ExPASy Swiss-Prot
Match: Q9LZS6 (Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana OX=3702 GN=SBT4.15 PE=3 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 4.5e-135
Identity = 278/564 (49.29%), Postives = 370/564 (65.60%), Query Frame = 0

Query: 25  DISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPSARVAMYKVCWSGTGCSDMDLLAA 84
           D  GHGTHTSS   G +V  ASL G+A GTARGGVPSAR+A YKVCW  +GC+DMD+LAA
Sbjct: 206 DHDGHGTHTSSTIAGVSVSSASLFGIANGTARGGVPSARIAAYKVCWD-SGCTDMDMLAA 265

Query: 85  FDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAMKKGIITVTAAGNTGPTAGSVVNH 144
           FD AI DGVD+ISISI G     + ED +AIGAFHAMK+GI+T  +AGN GP   +V N 
Sbjct: 266 FDEAISDGVDIISISIGGASL-PFFEDPIAIGAFHAMKRGILTTCSAGNNGPGLFTVSNL 325

Query: 145 APWILTVAASGIDRKFVSPFELGNGKNISGVGINLFNPNQKMYPLVSGGDVA-MNPQSKD 204
           APW++TVAA+ +DRKF +  +LGNG   SG+ +N FNP +KMYPL SG   + ++     
Sbjct: 326 APWVMTVAANSLDRKFETVVKLGNGLTASGISLNGFNPRKKMYPLTSGSLASNLSAGGYG 385

Query: 205 SASFCVDGTLDPSKVKGKLVFCKLIT-------WGADSVVKSIGAYGTIIQSDQFLPNTD 264
             S C  GTL   KV GK+V+C+           G D VV+S+   G I+   Q L  TD
Sbjct: 386 EPSTCEPGTLGEDKVMGKVVYCEAGREEGGNGGQGQDHVVRSLKGAGVIV---QLLEPTD 445

Query: 265 LFMAPASLVSSLV-----GETIDNYIHSTRAPTAVIYKSRQLKSAAPIVAFFSSRGPNPG 324
             MA ++L++        G  I  YI+ST+ P AVI+K++  K  AP ++ FS+RGP   
Sbjct: 446 --MATSTLIAGSYVFFEDGTKITEYINSTKNPQAVIFKTKTTKMLAPSISSFSARGPQRI 505

Query: 325 SNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKS 384
           S  ILKPDI+APG++ILA Y+ L S+TG   D + + F++MSGTSMACPH AAAAAYVKS
Sbjct: 506 SPNILKPDISAPGLNILAAYSKLASVTGYPDDNRRTLFSIMSGTSMACPHAAAAAAYVKS 565

Query: 385 FHPLWSPAAIRSALLTTASPVSRRLNPDGEFSYGAGNLSPSRARNPGLIYDLNEMSYIQF 444
           FHP WSPAAI+SAL+TTA+P+  + N + E SYG+G ++P RA +PGL+YD+ E +Y++F
Sbjct: 566 FHPDWSPAAIKSALMTTATPMRIKGN-EAELSYGSGQINPRRAIHPGLVYDITEDAYLRF 625

Query: 445 LCSEGYTGSSISILTG-------TKSIDCSTL-RGQGYDSLNYPTFQLSLKPTRQPTTAV 504
           LC EGY  +SI +LTG        K  +C  + RG G D LNYP+    +  T    + V
Sbjct: 626 LCKEGYNSTSIGLLTGDNSNNTTKKEYNCENIKRGLGSDGLNYPSLHKQVNSTEAKVSEV 685

Query: 505 FWRQVTNVGRPVSVYNATVRAPPGVEITVVPATLSFSQLLQKQNFKVIVKASPLPSAK-M 564
           F+R VTNVG   S Y A V AP G+ + VVP  +SF +  +K+NFKV++      + K +
Sbjct: 686 FYRTVTNVGYGPSTYVARVWAPKGLRVEVVPKVMSFERPKEKRNFKVVIDGVWDETMKGI 745

Query: 565 VSGSLTWAAAR-YVVRSPIVIYGS 566
           VS S+ W  +R ++VRSPI+++ S
Sbjct: 746 VSASVEWDDSRGHLVRSPILLFRS 761

BLAST of Lag0041274 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 443.4 bits (1139), Expect = 4.0e-123
Identity = 241/545 (44.22%), Postives = 354/545 (64.95%), Query Frame = 0

Query: 19  DILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPSARVAMYKVCWSGTGCSD 78
           D+  P D +GHGTHT+S A G  V  A+L GL  GTARGGVP AR+A YKVCW+  GCSD
Sbjct: 194 DVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAAYKVCWN-DGCSD 253

Query: 79  MDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAMKKGIITVTAAGNTGPTA 138
            D+LAA+D AI DGVD+IS+S+ G    +Y  DA+AIG+FHA+++GI+T  +AGN GP  
Sbjct: 254 TDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILTSNSAGNGGPNF 313

Query: 139 GSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISGVGINLFNPNQKMYPLVSGGDVAMN 198
            +  + +PW+L+VAAS +DRKFV+  ++GNG++  GV IN F  + + YPLVSG D+   
Sbjct: 314 FTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTF--DNQYYPLVSGRDIPNT 373

Query: 199 PQSKDSASFCVDGTLDPSKVKGKLVFCKLITWGADSVVKSIGAYGTIIQSDQFLPNTDLF 258
              K ++ FC D +++P+ +KGK+V C+  ++G     KS+     ++ +       D +
Sbjct: 374 GFDKSTSRFCTDKSVNPNLLKGKIVVCE-ASFGPHEFFKSLDGAAGVLMTSNTRDYADSY 433

Query: 259 MAPASLVSSLVGETIDNYIHSTRAPTAVIYKSRQ-LKSAAPIVAFFSSRGPNPGSNRILK 318
             P+S++          YI+S R+P A I+KS   L ++AP+V  FSSRGPN  +  ++K
Sbjct: 434 PLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIK 493

Query: 319 PDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWS 378
           PDI+ PGV+ILA +  +  + G++ +T F+   ++SGTSM+CPH+   A YVK+++P WS
Sbjct: 494 PDISGPGVEILAAWPSVAPVGGIRRNTLFN---IISGTSMSCPHITGIATYVKTYNPTWS 553

Query: 379 PAAIRSALLTTASPVSRRLNPDGEFSYGAGNLSPSRARNPGLIYDLNEMSYIQFLCSEGY 438
           PAAI+SAL+TTASP++ R NP  EF+YG+G+++P +A  PGL+YD NE  Y++FLC +GY
Sbjct: 554 PAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGY 613

Query: 439 TGSSISILTGTKSIDCSTLRGQGYDSLNYPTFQLSLKPTRQPTTAVFWRQVTNVGRPVSV 498
              ++  +TG  S   S   G+ +D LNYP+F LS+ P+ Q     F R +T+V    S 
Sbjct: 614 NTQAVRRITGDYSACTSGNTGRVWD-LNYPSFGLSVSPS-QTFNQYFNRTLTSVAPQAST 673

Query: 499 YNATVRAPPGVEITVVPATLSFSQLLQKQNFKVIVKASPLPSAKMVSGSLTWAAARYVVR 558
           Y A + AP G+ I+V P  LSF+ L  +++F + V+ S      +VS SL W+   + VR
Sbjct: 674 YRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGS--IKGFVVSASLVWSDGVHYVR 727

Query: 559 SPIVI 563
           SPI I
Sbjct: 734 SPITI 727

BLAST of Lag0041274 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 2.7e-119
Identity = 249/562 (44.31%), Postives = 358/562 (63.70%), Query Frame = 0

Query: 6   SKIFQARRQPRSLDILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPSARVA 65
           +K+  AR   +  D  S  D  GHGTHT+S A GNAV  AS  GLA GTARGGVPSAR+A
Sbjct: 175 NKLIGARFYNKFAD--SARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIA 234

Query: 66  MYKVCWSGTGCSDMDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAMKKGI 125
            YKVC++   C+D+D+LAAFD AI DGVDVISISI+ +   N    +VAIG+FHAM +GI
Sbjct: 235 AYKVCFN--RCNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGI 294

Query: 126 ITVTAAGNTGPTAGSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISGVGINLFNPNQK 185
           IT  +AGN GP  GSV N +PW++TVAASG DR+F+    LGNGK ++G+ +N FN N  
Sbjct: 295 ITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGT 354

Query: 186 MYPLVSGGDVAMNPQSKDSASFCVDGTLDPSKVKGKLVFCKLITWGADSVVKSIGAYGTI 245
            +P+V G +V+ N  S+  A +C  G +D   VKGK+V C       ++ +   GA G I
Sbjct: 355 KFPIVYGQNVSRN-CSQAQAGYCSSGCVDSELVKGKIVLCDDFLGYREAYL--AGAIGVI 414

Query: 246 IQSDQFLPNTDLFMAPASLVSSLVGETIDNYIHSTRAPTAVIYKSRQL-KSAAPIVAFFS 305
           +Q+     +  +   PAS +     ++I +YI S   P A I ++ ++    AP V  FS
Sbjct: 415 VQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFS 474

Query: 306 SRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKG--DTQFSKFTLMSGTSMACPHV 365
           SRGP+     +LKPD++APG++ILA ++P+ S +      D +  ++++MSGTSMACPHV
Sbjct: 475 SRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHV 534

Query: 366 AAAAAYVKSFHPLWSPAAIRSALLTTASPVSRRLNPDGEFSYGAGNLSPSRARNPGLIYD 425
           A  AAYVKSFHP WSP+AI+SA++TTA+P++ + NP+ EF+YG+G ++P++A +PGL+Y+
Sbjct: 535 AGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYE 594

Query: 426 LNEMSYIQFLCSEGYTGSSISILTGTKSIDCSTLRGQGYDSLNYPTFQLSLKPTRQPTTA 485
           +    Y++ LC+EG+  ++++  +G +++ CS  R +  D LNYPT   +   +  P   
Sbjct: 595 VETEDYLKMLCAEGFDSTTLTTTSG-QNVTCSE-RTEVKD-LNYPT-MTTFVSSLDPFNV 654

Query: 486 VFWRQVTNVGRPVSVYNAT-VRAPPGVEITVVPATLSFSQLLQKQNFKVIVKASPLPSAK 545
            F R VTNVG P S Y A+ V   P ++I++ P  L F  L +K++F V +    L    
Sbjct: 655 TFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFVVTISGKELKDGS 714

Query: 546 MVSGSLTWAAARYVVRSPIVIY 564
            VS S+ W+   + VRSPIV Y
Sbjct: 715 FVSSSVVWSDGSHSVRSPIVAY 725

BLAST of Lag0041274 vs. ExPASy Swiss-Prot
Match: Q9STF7 (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 3.3e-117
Identity = 241/544 (44.30%), Postives = 338/544 (62.13%), Query Frame = 0

Query: 25  DISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPSARVAMYKVCWSGT-GCSDMDLLA 84
           D +GHG+HT+SIA GNAV   S  GL  GT RGGVP+AR+A+YKVC  G   C+   +LA
Sbjct: 200 DNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVYKVCDPGVIRCTSDGILA 259

Query: 85  AFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAMKKGIITVTAAGNTGPTAGSVVN 144
           AFD AI D VD+I++S+  +  G + ED +AIGAFHAM KGI+TV  AGN GP   ++V+
Sbjct: 260 AFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGILTVNGAGNNGPERRTIVS 319

Query: 145 HAPWILTVAASGIDRKFVSPFELGNGKNISGVGINLFNPNQKMYPLVSGGDVAMNPQSKD 204
            APW+ TVAAS ++R F++   LGNGK I G  +N F+ N K YPLV G   A +     
Sbjct: 320 MAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKKYPLVYGKS-ASSRCDAS 379

Query: 205 SASFCVDGTLDPSKVKGKLVFCKLITWGADSVVKSIGAYGTIIQSDQFLPNTDLFMAPAS 264
           SA FC  G LD  +VKGK+V C   T       +++GA  +I++ + +     +F  P S
Sbjct: 380 SAGFCSPGCLDSKRVKGKIVLCD--TQRNPGEAQAMGAVASIVR-NPYEDAASVFSFPVS 439

Query: 265 LVSSLVGETIDNYIHSTRAPTAVIYKSRQL-KSAAPIVAFFSSRGPNPGSNRILKPDIAA 324
           ++S      + +Y++ST+ P A + KS  +    AP+VA +SSRGPNP  + ILKPDI A
Sbjct: 440 VLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSRGPNPLIHDILKPDITA 499

Query: 325 PGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIR 384
           PG +ILA Y+P   +   + DT+  K+T++SGTSM+CPHVA  AAY+K+FHPLWSP+ I+
Sbjct: 500 PGSEILAAYSPY--VPPSESDTRHVKYTVISGTSMSCPHVAGVAAYIKTFHPLWSPSMIQ 559

Query: 385 SALLTTASPVSRRLNPD---GEFSYGAGNLSPSRARNPGLIYDLNEMSYIQFLCSEGYTG 444
           SA++TTA P++   +P     EF+YGAG++ P  A +PGL+Y+ N+  +I FLC   YTG
Sbjct: 560 SAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEANKSDHITFLCGFNYTG 619

Query: 445 SSISILTGTKSIDCSTLRGQGYDSLNYPTFQLSLKPTRQPTTAVFWRQVTNVGRPVSVYN 504
             + +++G  S            +LNYP+    +  T+ P    F R VTNVGRP + Y 
Sbjct: 620 KKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQVSGTK-PFKVTFRRTVTNVGRPNATYK 679

Query: 505 ATVRAPPGVEITVVPATLSFSQLLQKQNFKVIVKASPLPSAKMVSGSLTWAAARYVVRSP 564
           A V     +++ VVPA LS   L +K++F V V  +   +  +VS  L W+   + VRSP
Sbjct: 680 AKV-VGSKLKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAENLVSAQLIWSDGVHFVRSP 735

BLAST of Lag0041274 vs. ExPASy TrEMBL
Match: A0A5D3E0J2 (Subtilisin-like protease SBT4.14 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold120G001460 PE=3 SV=1)

HSP 1 Score: 907.1 bits (2343), Expect = 3.6e-260
Identity = 456/547 (83.36%), Postives = 498/547 (91.04%), Query Frame = 0

Query: 18  LDILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPSARVAMYKVCWSGTGCS 77
           LDILSP+DI+GHGTHTSS ATGN + GA+LSGLA GTARGGVPSARVAMYKVCW+ TGCS
Sbjct: 55  LDILSPIDINGHGTHTSSTATGNVITGANLSGLAQGTARGGVPSARVAMYKVCWTSTGCS 114

Query: 78  DMDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAMKKGIITVTAAGNTGPT 137
           DMDLLAAFDAAIQDGVDVISISIAG G+GNYTED ++IGAFHAMKKGIITVTAAGN GP+
Sbjct: 115 DMDLLAAFDAAIQDGVDVISISIAGIGYGNYTEDPISIGAFHAMKKGIITVTAAGNNGPS 174

Query: 138 AGSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISGVGINLFNPNQKMYPLVSGGDVAM 197
           AG+VVNHAPWILTVAAS IDR F+SP ELGNGKNISGVGINLFNP +KMY LVSG DVA 
Sbjct: 175 AGTVVNHAPWILTVAASSIDRTFISPVELGNGKNISGVGINLFNPEKKMYKLVSGEDVAK 234

Query: 198 NPQSKDSASFCVDGTLDPSKVKGKLVFCKLITWGADSVVKSIGAYGTIIQSDQFLPNTDL 257
           N +SKD A++CV+ +LDPSKVK  +VFCKL+TWGADS VKSIGA G I+QSDQFL NTD+
Sbjct: 235 NIESKDDATYCVENSLDPSKVKDSIVFCKLLTWGADSAVKSIGAAGAILQSDQFLDNTDI 294

Query: 258 FMAPASLVSSLVGETIDNYIHSTRAPTAVIYKSRQLKSAAPIVAFFSSRGPNPGSNRILK 317
           FMAP++LVSS VG TID+YIHS+R PTAVIYK+RQ   AAPIVA FSSRGPNPGS RILK
Sbjct: 295 FMAPSTLVSSFVGATIDSYIHSSRTPTAVIYKTRQHIGAAPIVAPFSSRGPNPGSTRILK 354

Query: 318 PDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWS 377
           PDIAAPGV+ILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWS
Sbjct: 355 PDIAAPGVNILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWS 414

Query: 378 PAAIRSALLTTASPVSRRLNPDGEFSYGAGNLSPSRARNPGLIYDLNEMSYIQFLCSEGY 437
           PAAIRSALLTTA P+SRR NPDGEF YGAGNL+P +A+NPGLIYDLNEMSYIQFLCSEGY
Sbjct: 415 PAAIRSALLTTAKPISRRGNPDGEFGYGAGNLNPGKAKNPGLIYDLNEMSYIQFLCSEGY 474

Query: 438 TGSSISILTGTKSIDCST-LRGQGYDSLNYPTFQLSLKPTRQPTTAVFWRQVTNVGRPVS 497
           +GSSISILTGTKSI+C+T +RGQGYDSLNYPTFQL L+ TRQPTTAVFWR+VTNVG PVS
Sbjct: 475 SGSSISILTGTKSINCATIIRGQGYDSLNYPTFQLRLQSTRQPTTAVFWREVTNVGTPVS 534

Query: 498 VYNATVRAPPGVEITVVPATLSFSQLLQKQNFKVIVKASPLPSAKMVSGSLTWAAARYVV 557
           VYN TV APPGVEITV PATLSFS LLQK+ FKV+VKA+PLPS KMVSGS+TW   RYVV
Sbjct: 535 VYNTTVWAPPGVEITVEPATLSFSYLLQKERFKVVVKANPLPSNKMVSGSITWFNPRYVV 594

Query: 558 RSPIVIY 564
           RSPIV+Y
Sbjct: 595 RSPIVVY 601

BLAST of Lag0041274 vs. ExPASy TrEMBL
Match: A0A1S3BZ36 (subtilisin-like protease SBT4.14 OS=Cucumis melo OX=3656 GN=LOC103495168 PE=3 SV=1)

HSP 1 Score: 907.1 bits (2343), Expect = 3.6e-260
Identity = 456/547 (83.36%), Postives = 498/547 (91.04%), Query Frame = 0

Query: 18  LDILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPSARVAMYKVCWSGTGCS 77
           LDILSP+DI+GHGTHTSS ATGN + GA+LSGLA GTARGGVPSARVAMYKVCW+ TGCS
Sbjct: 227 LDILSPIDINGHGTHTSSTATGNVITGANLSGLAQGTARGGVPSARVAMYKVCWTSTGCS 286

Query: 78  DMDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAMKKGIITVTAAGNTGPT 137
           DMDLLAAFDAAIQDGVDVISISIAG G+GNYTED ++IGAFHAMKKGIITVTAAGN GP+
Sbjct: 287 DMDLLAAFDAAIQDGVDVISISIAGIGYGNYTEDPISIGAFHAMKKGIITVTAAGNNGPS 346

Query: 138 AGSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISGVGINLFNPNQKMYPLVSGGDVAM 197
           AG+VVNHAPWILTVAAS IDR F+SP ELGNGKNISGVGINLFNP +KMY LVSG DVA 
Sbjct: 347 AGTVVNHAPWILTVAASSIDRTFISPVELGNGKNISGVGINLFNPEKKMYKLVSGEDVAK 406

Query: 198 NPQSKDSASFCVDGTLDPSKVKGKLVFCKLITWGADSVVKSIGAYGTIIQSDQFLPNTDL 257
           N +SKD A++CV+ +LDPSKVK  +VFCKL+TWGADS VKSIGA G I+QSDQFL NTD+
Sbjct: 407 NIESKDDATYCVENSLDPSKVKDSIVFCKLLTWGADSAVKSIGAAGAILQSDQFLDNTDI 466

Query: 258 FMAPASLVSSLVGETIDNYIHSTRAPTAVIYKSRQLKSAAPIVAFFSSRGPNPGSNRILK 317
           FMAP++LVSS VG TID+YIHS+R PTAVIYK+RQ   AAPIVA FSSRGPNPGS RILK
Sbjct: 467 FMAPSTLVSSFVGATIDSYIHSSRTPTAVIYKTRQHIGAAPIVAPFSSRGPNPGSTRILK 526

Query: 318 PDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWS 377
           PDIAAPGV+ILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWS
Sbjct: 527 PDIAAPGVNILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWS 586

Query: 378 PAAIRSALLTTASPVSRRLNPDGEFSYGAGNLSPSRARNPGLIYDLNEMSYIQFLCSEGY 437
           PAAIRSALLTTA P+SRR NPDGEF YGAGNL+P +A+NPGLIYDLNEMSYIQFLCSEGY
Sbjct: 587 PAAIRSALLTTAKPISRRGNPDGEFGYGAGNLNPGKAKNPGLIYDLNEMSYIQFLCSEGY 646

Query: 438 TGSSISILTGTKSIDCST-LRGQGYDSLNYPTFQLSLKPTRQPTTAVFWRQVTNVGRPVS 497
           +GSSISILTGTKSI+C+T +RGQGYDSLNYPTFQL L+ TRQPTTAVFWR+VTNVG PVS
Sbjct: 647 SGSSISILTGTKSINCATIIRGQGYDSLNYPTFQLRLQSTRQPTTAVFWREVTNVGTPVS 706

Query: 498 VYNATVRAPPGVEITVVPATLSFSQLLQKQNFKVIVKASPLPSAKMVSGSLTWAAARYVV 557
           VYN TV APPGVEITV PATLSFS LLQK+ FKV+VKA+PLPS KMVSGS+TW   RYVV
Sbjct: 707 VYNTTVWAPPGVEITVEPATLSFSYLLQKERFKVVVKANPLPSNKMVSGSITWFNPRYVV 766

Query: 558 RSPIVIY 564
           RSPIV+Y
Sbjct: 767 RSPIVVY 773

BLAST of Lag0041274 vs. ExPASy TrEMBL
Match: A0A0A0KME6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G000010 PE=3 SV=1)

HSP 1 Score: 898.3 bits (2320), Expect = 1.7e-257
Identity = 448/559 (80.14%), Postives = 503/559 (89.98%), Query Frame = 0

Query: 6   SKIFQARRQPRSLDILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPSARVA 65
           ++ F+    P   DILSPVD  GHGTHTSS ATGNA+ GASLSGLA GTARGGVPSARVA
Sbjct: 183 ARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAIAGASLSGLAEGTARGGVPSARVA 242

Query: 66  MYKVCWSGTGCSDMDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAMKKGI 125
           MYKVCW+ +GCSDMD+LAAFDAAIQDGVDVISISI G GF NY++D+++IGAFHAMKKGI
Sbjct: 243 MYKVCWTSSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHAMKKGI 302

Query: 126 ITVTAAGNTGPTAGSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISGVGINLFNPNQK 185
           ITVT+AGN GPTAGSVVNHAPWI+TVAAS IDRKF+SP ELGNGKNISGVGIN+FNP QK
Sbjct: 303 ITVTSAGNGGPTAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIFNPKQK 362

Query: 186 MYPLVSGGDVAMNPQSKDSASFCVDGTLDPSKVKGKLVFCKLITWGADSVVKSIGAYGTI 245
           MYPLVSGGDVA N +SKD+ASFC++GTLDP+KVKG LVFCKL+TWGADSV+KSIGA G I
Sbjct: 363 MYPLVSGGDVARNSESKDTASFCLEGTLDPTKVKGSLVFCKLLTWGADSVIKSIGANGVI 422

Query: 246 IQSDQFLPNTDLFMAPASLVSSLVGETIDNYIHSTRAPTAVIYKSRQLKSAAPIVAFFSS 305
           IQSD+FL N D+FMAPA++VSSLVG  I  YI STR PTAVIYK++QLK+ AP+VA FSS
Sbjct: 423 IQSDEFLDNADIFMAPATMVSSLVGNIIYTYIKSTRTPTAVIYKTKQLKAKAPMVASFSS 482

Query: 306 RGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAA 365
           RGPNPGS+RILKPDIAAPGVDILA YTPLKSLTG KGDTQ+SKFTLMSGTSMACPHVAAA
Sbjct: 483 RGPNPGSHRILKPDIAAPGVDILAAYTPLKSLTGQKGDTQYSKFTLMSGTSMACPHVAAA 542

Query: 366 AAYVKSFHPLWSPAAIRSALLTTASPVSRRLNPDGEFSYGAGNLSPSRARNPGLIYDLNE 425
           AAYVKSFHPLWSPAAIRSALLTTA+P+SRRLNP+GEF+YGAGNL+PSRA +PGLIYDLNE
Sbjct: 543 AAYVKSFHPLWSPAAIRSALLTTATPISRRLNPEGEFAYGAGNLNPSRAISPGLIYDLNE 602

Query: 426 MSYIQFLCSEGYTGSSISILTGTKSIDCSTL-RGQGYDSLNYPTFQLSLKPTRQPTTAVF 485
           +SYIQFLCSEGYTGSSI++L+GTKSI+CS L  GQG+DSLNYPTFQLSLK T QP T  F
Sbjct: 603 ISYIQFLCSEGYTGSSIAVLSGTKSINCSNLIPGQGHDSLNYPTFQLSLKSTNQPMTTTF 662

Query: 486 WRQVTNVGRPVSVYNATVRAPPGVEITVVPATLSFSQLLQKQNFKVIVKASPLPSAKMVS 545
            R+VTNVG P+SVYNAT+ APPGV ITV P TLSFS+LLQK++FKV+VKASPLPSAKMVS
Sbjct: 663 RRRVTNVGHPISVYNATINAPPGVTITVTPPTLSFSRLLQKRSFKVVVKASPLPSAKMVS 722

Query: 546 GSLTWAAARYVVRSPIVIY 564
           GSL W  A++VVRSPIV+Y
Sbjct: 723 GSLAWVGAQHVVRSPIVVY 741

BLAST of Lag0041274 vs. ExPASy TrEMBL
Match: A0A5A7UKY8 (Subtilisin-like protease SBT4.14 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold186G002590 PE=3 SV=1)

HSP 1 Score: 887.1 bits (2291), Expect = 3.8e-254
Identity = 443/559 (79.25%), Postives = 500/559 (89.45%), Query Frame = 0

Query: 6   SKIFQARRQPRSLDILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPSARVA 65
           ++ F+    P   DILSPVD  GHGTHTSS ATGNAV GASLSGLA GTARGGVPSARVA
Sbjct: 186 ARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAVAGASLSGLAEGTARGGVPSARVA 245

Query: 66  MYKVCWSGTGCSDMDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAMKKGI 125
           MYKVCW+ +GCSDMD+LAAFDAAIQDGVDVISISI G GF NY++D+++IGAFHAMKKGI
Sbjct: 246 MYKVCWASSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHAMKKGI 305

Query: 126 ITVTAAGNTGPTAGSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISGVGINLFNPNQK 185
           ITVT+AGN GP AGSVVNHAPWI+TVAAS IDRKF+SP ELGNGKNISGVGIN+FNP +K
Sbjct: 306 ITVTSAGNGGPMAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIFNPKKK 365

Query: 186 MYPLVSGGDVAMNPQSKDSASFCVDGTLDPSKVKGKLVFCKLITWGADSVVKSIGAYGTI 245
           MYPLVSGGDVA N +SKD+ASFC++ +LDP+KVKG LVFCKL+TWGADSV+KS+GA G I
Sbjct: 366 MYPLVSGGDVARNSESKDTASFCLEDSLDPTKVKGSLVFCKLLTWGADSVIKSVGANGVI 425

Query: 246 IQSDQFLPNTDLFMAPASLVSSLVGETIDNYIHSTRAPTAVIYKSRQLKSAAPIVAFFSS 305
           IQSDQFL N D+FMAPA++VSS VG  I  YI STR PTAVIYK+RQLK+ AP+VA FSS
Sbjct: 426 IQSDQFLDNADIFMAPATMVSSSVGTIIYTYIKSTRTPTAVIYKTRQLKAKAPMVASFSS 485

Query: 306 RGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAA 365
           RGPNPGS+RILKPDIAAPGV+ILAGYTPLKSLTG KGDTQ+SKFT+MSGTSMACPHVAAA
Sbjct: 486 RGPNPGSHRILKPDIAAPGVNILAGYTPLKSLTGQKGDTQYSKFTIMSGTSMACPHVAAA 545

Query: 366 AAYVKSFHPLWSPAAIRSALLTTASPVSRRLNPDGEFSYGAGNLSPSRARNPGLIYDLNE 425
           AAYVKSFHPLWSPAAIRSALLTT  P+SRRLNP+GEF+YGAGNL+PS+A +PGLIYDLNE
Sbjct: 546 AAYVKSFHPLWSPAAIRSALLTTTRPISRRLNPEGEFAYGAGNLNPSKAISPGLIYDLNE 605

Query: 426 MSYIQFLCSEGYTGSSISILTGTKSIDCSTL-RGQGYDSLNYPTFQLSLKPTRQPTTAVF 485
           MSYIQFLCSEGYTGSSI++L GTKSI+CSTL  G G+DSLNYPTFQLSL+ T+QP T  F
Sbjct: 606 MSYIQFLCSEGYTGSSIAVLAGTKSINCSTLIPGHGHDSLNYPTFQLSLRSTKQPMTTTF 665

Query: 486 WRQVTNVGRPVSVYNATVRAPPGVEITVVPATLSFSQLLQKQNFKVIVKASPLPSAKMVS 545
            RQVTNVG+PVSVYNAT++APPGV+ITV P TLSFS+LLQK++FKV+VKASPL SAKMVS
Sbjct: 666 RRQVTNVGQPVSVYNATIKAPPGVKITVTPTTLSFSRLLQKRSFKVVVKASPLQSAKMVS 725

Query: 546 GSLTWAAARYVVRSPIVIY 564
           GSL W   R+VVRSPIV+Y
Sbjct: 726 GSLAWVGDRHVVRSPIVVY 744

BLAST of Lag0041274 vs. ExPASy TrEMBL
Match: A0A5D3DZV3 (Subtilisin-like protease SBT4.14 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold120G001620 PE=3 SV=1)

HSP 1 Score: 887.1 bits (2291), Expect = 3.8e-254
Identity = 443/559 (79.25%), Postives = 500/559 (89.45%), Query Frame = 0

Query: 6   SKIFQARRQPRSLDILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPSARVA 65
           ++ F+    P   DILSPVD  GHGTHTSS ATGNAV GASLSGLA GTARGGVPSARVA
Sbjct: 111 ARYFKLDGNPDPSDILSPVDTDGHGTHTSSTATGNAVAGASLSGLAEGTARGGVPSARVA 170

Query: 66  MYKVCWSGTGCSDMDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAMKKGI 125
           MYKVCW+ +GCSDMD+LAAFDAAIQDGVDVISISI G GF NY++D+++IGAFHAMKKGI
Sbjct: 171 MYKVCWASSGCSDMDILAAFDAAIQDGVDVISISIGGGGFNNYSDDSISIGAFHAMKKGI 230

Query: 126 ITVTAAGNTGPTAGSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISGVGINLFNPNQK 185
           ITVT+AGN GP AGSVVNHAPWI+TVAAS IDRKF+SP ELGNGKNISGVGIN+FNP +K
Sbjct: 231 ITVTSAGNGGPMAGSVVNHAPWIVTVAASSIDRKFISPLELGNGKNISGVGINIFNPKKK 290

Query: 186 MYPLVSGGDVAMNPQSKDSASFCVDGTLDPSKVKGKLVFCKLITWGADSVVKSIGAYGTI 245
           MYPLVSGGDVA N +SKD+ASFC++ +LDP+KVKG LVFCKL+TWGADSV+KS+GA G I
Sbjct: 291 MYPLVSGGDVARNSESKDTASFCLEDSLDPTKVKGSLVFCKLLTWGADSVIKSVGANGVI 350

Query: 246 IQSDQFLPNTDLFMAPASLVSSLVGETIDNYIHSTRAPTAVIYKSRQLKSAAPIVAFFSS 305
           IQSDQFL N D+FMAPA++VSS VG  I  YI STR PTAVIYK+RQLK+ AP+VA FSS
Sbjct: 351 IQSDQFLDNADIFMAPATMVSSSVGTIIYTYIKSTRTPTAVIYKTRQLKAKAPMVASFSS 410

Query: 306 RGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAA 365
           RGPNPGS+RILKPDIAAPGV+ILAGYTPLKSLTG KGDTQ+SKFT+MSGTSMACPHVAAA
Sbjct: 411 RGPNPGSHRILKPDIAAPGVNILAGYTPLKSLTGQKGDTQYSKFTIMSGTSMACPHVAAA 470

Query: 366 AAYVKSFHPLWSPAAIRSALLTTASPVSRRLNPDGEFSYGAGNLSPSRARNPGLIYDLNE 425
           AAYVKSFHPLWSPAAIRSALLTT  P+SRRLNP+GEF+YGAGNL+PS+A +PGLIYDLNE
Sbjct: 471 AAYVKSFHPLWSPAAIRSALLTTTRPISRRLNPEGEFAYGAGNLNPSKAISPGLIYDLNE 530

Query: 426 MSYIQFLCSEGYTGSSISILTGTKSIDCSTL-RGQGYDSLNYPTFQLSLKPTRQPTTAVF 485
           MSYIQFLCSEGYTGSSI++L GTKSI+CSTL  G G+DSLNYPTFQLSL+ T+QP T  F
Sbjct: 531 MSYIQFLCSEGYTGSSIAVLAGTKSINCSTLIPGHGHDSLNYPTFQLSLRSTKQPMTTTF 590

Query: 486 WRQVTNVGRPVSVYNATVRAPPGVEITVVPATLSFSQLLQKQNFKVIVKASPLPSAKMVS 545
            RQVTNVG+PVSVYNAT++APPGV+ITV P TLSFS+LLQK++FKV+VKASPL SAKMVS
Sbjct: 591 RRQVTNVGQPVSVYNATIKAPPGVKITVTPTTLSFSRLLQKRSFKVVVKASPLQSAKMVS 650

Query: 546 GSLTWAAARYVVRSPIVIY 564
           GSL W   R+VVRSPIV+Y
Sbjct: 651 GSLAWVGDRHVVRSPIVVY 669

BLAST of Lag0041274 vs. TAIR 10
Match: AT4G00230.1 (xylem serine peptidase 1 )

HSP 1 Score: 664.1 bits (1712), Expect = 1.0e-190
Identity = 330/546 (60.44%), Postives = 424/546 (77.66%), Query Frame = 0

Query: 19  DILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPSARVAMYKVCWSGTGCSD 78
           ++ SP+DI GHGTHTSS   G  V  ASL G+A GTARG VPSAR+AMYKVCW+ +GC+D
Sbjct: 200 EVRSPIDIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVPSARLAMYKVCWARSGCAD 259

Query: 79  MDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAMKKGIITVTAAGNTGPTA 138
           MD+LA F+AAI DGV++ISISI G    +Y+ D++++G+FHAM+KGI+TV +AGN GP++
Sbjct: 260 MDILAGFEAAIHDGVEIISISI-GGPIADYSSDSISVGSFHAMRKGILTVASAGNDGPSS 319

Query: 139 GSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISGVGINLFNPNQKMYPLVSGGDVAMN 198
           G+V NH PWILTVAASGIDR F S  +LGNGK+ SG+GI++F+P  K YPLVSG D A N
Sbjct: 320 GTVTNHEPWILTVAASGIDRTFKSKIDLGNGKSFSGMGISMFSPKAKSYPLVSGVDAAKN 379

Query: 199 PQSKDSASFCVDGTLDPSKVKGKLVFCKLITWGADSVVKSIGAYGTIIQSDQFLPNTDLF 258
              K  A +C   +LD  KVKGK++ C++   G +S +KS G  G II SDQ+L N  +F
Sbjct: 380 TDDKYLARYCFSDSLDRKKVKGKVMVCRMGGGGVESTIKSYGGAGAIIVSDQYLDNAQIF 439

Query: 259 MAPASLVSSLVGETIDNYIHSTRAPTAVIYKSRQLKSAAPIVAFFSSRGPNPGSNRILKP 318
           MAPA+ V+S VG+ I  YI+STR+ +AVI K+RQ+   AP VA FSSRGPNPGS R+LKP
Sbjct: 440 MAPATSVNSSVGDIIYRYINSTRSASAVIQKTRQVTIPAPFVASFSSRGPNPGSIRLLKP 499

Query: 319 DIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSP 378
           DIAAPG+DILA +T  +SLTGL GDTQFSKFT++SGTSMACPHVA  AAYVKSFHP W+P
Sbjct: 500 DIAAPGIDILAAFTLKRSLTGLDGDTQFSKFTILSGTSMACPHVAGVAAYVKSFHPDWTP 559

Query: 379 AAIRSALLTTASPVSRRLNPDGEFSYGAGNLSPSRARNPGLIYDLNEMSYIQFLCSEGYT 438
           AAI+SA++T+A P+SRR+N D EF+YG G ++P RA +PGL+YD++++SY+QFLC EGY 
Sbjct: 560 AAIKSAIITSAKPISRRVNKDAEFAYGGGQINPRRAASPGLVYDMDDISYVQFLCGEGYN 619

Query: 439 GSSISILTGTKSIDCSTL-RGQGYDSLNYPTFQLSLKPTRQPTTAVFWRQVTNVGRPVSV 498
            ++++ L GT+S+ CS++  G G+DSLNYPT QL+L+  +  T AVF R+VTNVG P SV
Sbjct: 620 ATTLAPLVGTRSVSCSSIVPGLGHDSLNYPTIQLTLRSAKTSTLAVFRRRVTNVGPPSSV 679

Query: 499 YNATVRAPPGVEITVVPATLSFSQLLQKQNFKVIVKASPLPSAKMVSGSLTWAAARYVVR 558
           Y ATVRAP GVEITV P +LSFS+  QK++FKV+VKA  +   K+VSG L W + R+ VR
Sbjct: 680 YTATVRAPKGVEITVEPQSLSFSKASQKRSFKVVVKAKQMTPGKIVSGLLVWKSPRHSVR 739

Query: 559 SPIVIY 564
           SPIVIY
Sbjct: 740 SPIVIY 744

BLAST of Lag0041274 vs. TAIR 10
Match: AT5G03620.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 483.0 bits (1242), Expect = 3.2e-136
Identity = 278/564 (49.29%), Postives = 370/564 (65.60%), Query Frame = 0

Query: 25  DISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPSARVAMYKVCWSGTGCSDMDLLAA 84
           D  GHGTHTSS   G +V  ASL G+A GTARGGVPSAR+A YKVCW  +GC+DMD+LAA
Sbjct: 206 DHDGHGTHTSSTIAGVSVSSASLFGIANGTARGGVPSARIAAYKVCWD-SGCTDMDMLAA 265

Query: 85  FDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAMKKGIITVTAAGNTGPTAGSVVNH 144
           FD AI DGVD+ISISI G     + ED +AIGAFHAMK+GI+T  +AGN GP   +V N 
Sbjct: 266 FDEAISDGVDIISISIGGASL-PFFEDPIAIGAFHAMKRGILTTCSAGNNGPGLFTVSNL 325

Query: 145 APWILTVAASGIDRKFVSPFELGNGKNISGVGINLFNPNQKMYPLVSGGDVA-MNPQSKD 204
           APW++TVAA+ +DRKF +  +LGNG   SG+ +N FNP +KMYPL SG   + ++     
Sbjct: 326 APWVMTVAANSLDRKFETVVKLGNGLTASGISLNGFNPRKKMYPLTSGSLASNLSAGGYG 385

Query: 205 SASFCVDGTLDPSKVKGKLVFCKLIT-------WGADSVVKSIGAYGTIIQSDQFLPNTD 264
             S C  GTL   KV GK+V+C+           G D VV+S+   G I+   Q L  TD
Sbjct: 386 EPSTCEPGTLGEDKVMGKVVYCEAGREEGGNGGQGQDHVVRSLKGAGVIV---QLLEPTD 445

Query: 265 LFMAPASLVSSLV-----GETIDNYIHSTRAPTAVIYKSRQLKSAAPIVAFFSSRGPNPG 324
             MA ++L++        G  I  YI+ST+ P AVI+K++  K  AP ++ FS+RGP   
Sbjct: 446 --MATSTLIAGSYVFFEDGTKITEYINSTKNPQAVIFKTKTTKMLAPSISSFSARGPQRI 505

Query: 325 SNRILKPDIAAPGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKS 384
           S  ILKPDI+APG++ILA Y+ L S+TG   D + + F++MSGTSMACPH AAAAAYVKS
Sbjct: 506 SPNILKPDISAPGLNILAAYSKLASVTGYPDDNRRTLFSIMSGTSMACPHAAAAAAYVKS 565

Query: 385 FHPLWSPAAIRSALLTTASPVSRRLNPDGEFSYGAGNLSPSRARNPGLIYDLNEMSYIQF 444
           FHP WSPAAI+SAL+TTA+P+  + N + E SYG+G ++P RA +PGL+YD+ E +Y++F
Sbjct: 566 FHPDWSPAAIKSALMTTATPMRIKGN-EAELSYGSGQINPRRAIHPGLVYDITEDAYLRF 625

Query: 445 LCSEGYTGSSISILTG-------TKSIDCSTL-RGQGYDSLNYPTFQLSLKPTRQPTTAV 504
           LC EGY  +SI +LTG        K  +C  + RG G D LNYP+    +  T    + V
Sbjct: 626 LCKEGYNSTSIGLLTGDNSNNTTKKEYNCENIKRGLGSDGLNYPSLHKQVNSTEAKVSEV 685

Query: 505 FWRQVTNVGRPVSVYNATVRAPPGVEITVVPATLSFSQLLQKQNFKVIVKASPLPSAK-M 564
           F+R VTNVG   S Y A V AP G+ + VVP  +SF +  +K+NFKV++      + K +
Sbjct: 686 FYRTVTNVGYGPSTYVARVWAPKGLRVEVVPKVMSFERPKEKRNFKVVIDGVWDETMKGI 745

Query: 565 VSGSLTWAAAR-YVVRSPIVIYGS 566
           VS S+ W  +R ++VRSPI+++ S
Sbjct: 746 VSASVEWDDSRGHLVRSPILLFRS 761

BLAST of Lag0041274 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 430.6 bits (1106), Expect = 1.9e-120
Identity = 249/562 (44.31%), Postives = 358/562 (63.70%), Query Frame = 0

Query: 6   SKIFQARRQPRSLDILSPVDISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPSARVA 65
           +K+  AR   +  D  S  D  GHGTHT+S A GNAV  AS  GLA GTARGGVPSAR+A
Sbjct: 139 NKLIGARFYNKFAD--SARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIA 198

Query: 66  MYKVCWSGTGCSDMDLLAAFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAMKKGI 125
            YKVC++   C+D+D+LAAFD AI DGVDVISISI+ +   N    +VAIG+FHAM +GI
Sbjct: 199 AYKVCFN--RCNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGI 258

Query: 126 ITVTAAGNTGPTAGSVVNHAPWILTVAASGIDRKFVSPFELGNGKNISGVGINLFNPNQK 185
           IT  +AGN GP  GSV N +PW++TVAASG DR+F+    LGNGK ++G+ +N FN N  
Sbjct: 259 ITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGT 318

Query: 186 MYPLVSGGDVAMNPQSKDSASFCVDGTLDPSKVKGKLVFCKLITWGADSVVKSIGAYGTI 245
            +P+V G +V+ N  S+  A +C  G +D   VKGK+V C       ++ +   GA G I
Sbjct: 319 KFPIVYGQNVSRN-CSQAQAGYCSSGCVDSELVKGKIVLCDDFLGYREAYL--AGAIGVI 378

Query: 246 IQSDQFLPNTDLFMAPASLVSSLVGETIDNYIHSTRAPTAVIYKSRQL-KSAAPIVAFFS 305
           +Q+     +  +   PAS +     ++I +YI S   P A I ++ ++    AP V  FS
Sbjct: 379 VQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFS 438

Query: 306 SRGPNPGSNRILKPDIAAPGVDILAGYTPLKSLTGLKG--DTQFSKFTLMSGTSMACPHV 365
           SRGP+     +LKPD++APG++ILA ++P+ S +      D +  ++++MSGTSMACPHV
Sbjct: 439 SRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHV 498

Query: 366 AAAAAYVKSFHPLWSPAAIRSALLTTASPVSRRLNPDGEFSYGAGNLSPSRARNPGLIYD 425
           A  AAYVKSFHP WSP+AI+SA++TTA+P++ + NP+ EF+YG+G ++P++A +PGL+Y+
Sbjct: 499 AGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYE 558

Query: 426 LNEMSYIQFLCSEGYTGSSISILTGTKSIDCSTLRGQGYDSLNYPTFQLSLKPTRQPTTA 485
           +    Y++ LC+EG+  ++++  +G +++ CS  R +  D LNYPT   +   +  P   
Sbjct: 559 VETEDYLKMLCAEGFDSTTLTTTSG-QNVTCSE-RTEVKD-LNYPT-MTTFVSSLDPFNV 618

Query: 486 VFWRQVTNVGRPVSVYNAT-VRAPPGVEITVVPATLSFSQLLQKQNFKVIVKASPLPSAK 545
            F R VTNVG P S Y A+ V   P ++I++ P  L F  L +K++F V +    L    
Sbjct: 619 TFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFVVTISGKELKDGS 678

Query: 546 MVSGSLTWAAARYVVRSPIVIY 564
            VS S+ W+   + VRSPIV Y
Sbjct: 679 FVSSSVVWSDGSHSVRSPIVAY 689

BLAST of Lag0041274 vs. TAIR 10
Match: AT3G46850.1 (Subtilase family protein )

HSP 1 Score: 423.7 bits (1088), Expect = 2.3e-118
Identity = 241/544 (44.30%), Postives = 338/544 (62.13%), Query Frame = 0

Query: 25  DISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPSARVAMYKVCWSGT-GCSDMDLLA 84
           D +GHG+HT+SIA GNAV   S  GL  GT RGGVP+AR+A+YKVC  G   C+   +LA
Sbjct: 200 DNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVYKVCDPGVIRCTSDGILA 259

Query: 85  AFDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAMKKGIITVTAAGNTGPTAGSVVN 144
           AFD AI D VD+I++S+  +  G + ED +AIGAFHAM KGI+TV  AGN GP   ++V+
Sbjct: 260 AFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGILTVNGAGNNGPERRTIVS 319

Query: 145 HAPWILTVAASGIDRKFVSPFELGNGKNISGVGINLFNPNQKMYPLVSGGDVAMNPQSKD 204
            APW+ TVAAS ++R F++   LGNGK I G  +N F+ N K YPLV G   A +     
Sbjct: 320 MAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKKYPLVYGKS-ASSRCDAS 379

Query: 205 SASFCVDGTLDPSKVKGKLVFCKLITWGADSVVKSIGAYGTIIQSDQFLPNTDLFMAPAS 264
           SA FC  G LD  +VKGK+V C   T       +++GA  +I++ + +     +F  P S
Sbjct: 380 SAGFCSPGCLDSKRVKGKIVLCD--TQRNPGEAQAMGAVASIVR-NPYEDAASVFSFPVS 439

Query: 265 LVSSLVGETIDNYIHSTRAPTAVIYKSRQL-KSAAPIVAFFSSRGPNPGSNRILKPDIAA 324
           ++S      + +Y++ST+ P A + KS  +    AP+VA +SSRGPNP  + ILKPDI A
Sbjct: 440 VLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSRGPNPLIHDILKPDITA 499

Query: 325 PGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIR 384
           PG +ILA Y+P   +   + DT+  K+T++SGTSM+CPHVA  AAY+K+FHPLWSP+ I+
Sbjct: 500 PGSEILAAYSPY--VPPSESDTRHVKYTVISGTSMSCPHVAGVAAYIKTFHPLWSPSMIQ 559

Query: 385 SALLTTASPVSRRLNPD---GEFSYGAGNLSPSRARNPGLIYDLNEMSYIQFLCSEGYTG 444
           SA++TTA P++   +P     EF+YGAG++ P  A +PGL+Y+ N+  +I FLC   YTG
Sbjct: 560 SAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEANKSDHITFLCGFNYTG 619

Query: 445 SSISILTGTKSIDCSTLRGQGYDSLNYPTFQLSLKPTRQPTTAVFWRQVTNVGRPVSVYN 504
             + +++G  S            +LNYP+    +  T+ P    F R VTNVGRP + Y 
Sbjct: 620 KKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQVSGTK-PFKVTFRRTVTNVGRPNATYK 679

Query: 505 ATVRAPPGVEITVVPATLSFSQLLQKQNFKVIVKASPLPSAKMVSGSLTWAAARYVVRSP 564
           A V     +++ VVPA LS   L +K++F V V  +   +  +VS  L W+   + VRSP
Sbjct: 680 AKV-VGSKLKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAENLVSAQLIWSDGVHFVRSP 735

BLAST of Lag0041274 vs. TAIR 10
Match: AT5G59100.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 413.3 bits (1061), Expect = 3.1e-115
Identity = 245/549 (44.63%), Postives = 340/549 (61.93%), Query Frame = 0

Query: 25  DISGHGTHTSSIATGNAVPGASLSGLAAGTARGGVPSARVAMYKVCWSGTGCSDMDLLAA 84
           D SGHGTHT+SIA GNAV  ++  GL  GTARGGVP+AR+A+YKVC    GC    +++A
Sbjct: 200 DYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYKVC-DNEGCDGEAMMSA 259

Query: 85  FDAAIQDGVDVISISIAGNGFGNYTEDAVAIGAFHAMKKGIITVTAAGNTGPTAGSVVNH 144
           FD AI DGVDVISISI  +    + ED +AIGAFHAM  G++TV AAGN GP   +V + 
Sbjct: 260 FDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTVNAAGNNGPKISTVTST 319

Query: 145 APWILTVAASGIDRKFVSPFELGNGKNISGVGINLFNPNQKMYPLVSGGDVAMNPQSKDS 204
           APW+ +VAAS  +R F++   LG+GK + G  +N ++ N   YPLV G   A++  S D 
Sbjct: 320 APWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPLVYGKSAALSTCSVDK 379

Query: 205 ASFCVDGTLDPSKVKGKLVFCKLITWGADSVVKSIGAYGTIIQSDQFLPNTDLFMA-PAS 264
           A  C    LD   VKGK+V C   T G     K +GA G+I+++ +  P+     + P S
Sbjct: 380 ARLCEPKCLDGKLVKGKIVLCD-STKGLIEAQK-LGAVGSIVKNPE--PDRAFIRSFPVS 439

Query: 265 LVSSLVGETIDNYIHSTRAPTAVIYKSRQLKS-AAPIVAFFSSRGPNPGSNRILKPDIAA 324
            +S+   +++ +Y++ST+ P A + KS ++ +  AP+VA FSSRGP+   + ILKPDI A
Sbjct: 440 FLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSRGPSSIVSDILKPDITA 499

Query: 325 PGVDILAGYTPLKSLTGLKGDTQFSKFTLMSGTSMACPHVAAAAAYVKSFHPLWSPAAIR 384
           PGV+ILA Y+P  S T  + DT+  K++++SGTSMACPHVA  AAYVK+FHP WSP+ I+
Sbjct: 500 PGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYVKTFHPQWSPSMIQ 559

Query: 385 SALLTTASPVSRRLNPDG------EFSYGAGNLSPSRARNPGLIYDLNEMSYIQFLCSEG 444
           SA++TTA P    +N  G      EF+YG+G++ P  A NPGL+Y+L +  +I FLC   
Sbjct: 560 SAIMTTAWP----MNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKADHINFLCGLN 619

Query: 445 YTGSSISILTGTKSIDCSTLRGQGYDSLNYPTFQLSLKPTRQPTTAVFWRQVTNVGRPVS 504
           YT   + I++G  S     +      +LNYPT    +  T+ P    F R VTNVG   S
Sbjct: 620 YTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGTK-PFNITFQRTVTNVGMQKS 679

Query: 505 VYNATVRAPPG--VEITVVPATLSFSQLLQKQNFKVIVKASPLPSAKMVSGSLTWAAARY 564
            YNA V   PG  + I V P  LS   + +KQ+F V V +  + + + VS +L W+   +
Sbjct: 680 TYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQPVSANLIWSDGTH 738