Lag0041267 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0041267 vs. NCBI nr
Match: XP_022941552.1 (subtilisin-like protease SBT1.1 [Cucurbita moschata] >XP_022941553.1 subtilisin-like protease SBT1.1 [Cucurbita moschata])

HSP 1 Score: 1192.2 bits (3083), Expect = 0.0e+00
Identity = 597/779 (76.64%), Postives = 675/779 (86.65%), Query Frame = 0

Query: 1   MAFREVWVLLCIVFAASTAALDQQTYIIHMDTTKMPTTNPQQWYTSMIDSVNELASLDED 60
           M FREV + L I  A STAA+DQQ+YIIHMDTTKM    P+QWYT++IDS+NE++SL ED
Sbjct: 1   MGFREVCLFLSIFLATSTAAVDQQSYIIHMDTTKMAAPTPEQWYTALIDSINEISSL-ED 60

Query: 61  EEEVSNAAEIFYVYKNAISGKELKCVLDKYKNNGFAAKLSTKKLNSLSQTPGFLTAIPDQ 120
           +EE SNAA+I YVYK AIS              GFAAKLSTKKL+SLS+TPGFL A P++
Sbjct: 61  QEEASNAAQILYVYKTAIS--------------GFAAKLSTKKLHSLSKTPGFLAATPNE 120

Query: 121 LLQLHTTHSPHFLGLKTGHGLWNATNLASDVIVGVVDTGIWPEHVSFEDKGLPPVPRRWK 180
           LLQLHTTHSP FLGL+  HGLWN++NLASD+++G++DTGIWPEH+SF+DKGLPPVP++WK
Sbjct: 121 LLQLHTTHSPQFLGLQREHGLWNSSNLASDIVIGLLDTGIWPEHISFQDKGLPPVPKKWK 180

Query: 181 GGCEEGQKFSASNCNRKLIGARAFIKAYEASFGRLNESGVFRSPRDWNGHGTHTASTAAG 240
           G C+ GQKFS SNCNRKLIGA A+IK YEA  GRLN +G FRSPRD +GHGTHTASTAAG
Sbjct: 181 GTCQAGQKFSPSNCNRKLIGAIAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAG 240

Query: 241 NLVFKAGFYNHALGNAAGIRFTSRIAAYKVCWPLGCAIADVLAAMDRAVADGVDVLSLSL 300
           N+V KA F+N A+G A G+RFTSRIAAYKVCW  GCA AD+LAA+DRAVADGVDVLSLSL
Sbjct: 241 NIVNKASFFNQAMGVATGMRFTSRIAAYKVCWTEGCANADILAAIDRAVADGVDVLSLSL 300

Query: 301 GGNPVPFYNDGLAIAAFGATQKGVFVSCAAGNSGPAPSTAGNLAPWIMTVGASYTDRIFP 360
           GG+   FY D +AIA FGA + GVFVSC+AGNSGP+ ST  N+APWIMTV ASYTDR FP
Sbjct: 301 GGSASAFYKDDIAIATFGAVRNGVFVSCSAGNSGPSSSTVSNIAPWIMTVAASYTDRSFP 360

Query: 361 TTVKLGNGQVFEGSSLFSGNNMGQLPLVYKQ--GRQGANLCLAGSLVPSMVRGKIVICER 420
            TVKLGNGQVFEGSSL+SGNN+GQLPLVY    G + AN+C AGSLVPSMV+GKIV+CER
Sbjct: 361 ATVKLGNGQVFEGSSLYSGNNIGQLPLVYNNTAGGEDANVCTAGSLVPSMVKGKIVVCER 420

Query: 421 GTNTRIGKGEQVKLAGGAGMILINTQLEGEELFADPHVLPATSLGAAAGKAIKNYIASSK 480
           GTN+R  KGEQVKLAGGAGMILINTQLEGEELFADPHVLPA +LGA+AG+AI  YI+SSK
Sbjct: 421 GTNSRTAKGEQVKLAGGAGMILINTQLEGEELFADPHVLPAANLGASAGQAIIRYISSSK 480

Query: 481 -QPKASIAFQGTKYGSRAPRVAAFSSRGPSSIEPNIIKPDIIAPGVNILAAWPSIVSPSE 540
            QPKA IAF+GTK+G+RAPRVAAFSSRGPS I P++IKPD+ APGVNILAAWP I SPSE
Sbjct: 481 HQPKALIAFEGTKFGNRAPRVAAFSSRGPSLIAPDVIKPDVTAPGVNILAAWPLIASPSE 540

Query: 541 LEFDKRRVLFNIVSGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTNDNKMSPI 600
           +E DKRRVLFN++SGTSMSCPHVSGLAALLK+AHKDWSPAAIKSALMTTAYTNDN+MSPI
Sbjct: 541 VESDKRRVLFNVISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNRMSPI 600

Query: 601 SDVGSANGKPANPFAFGSGHVDPEKASDPGLVYDITPEDYLNYLCSLNYNSTQIALVSRG 660
           SDVGSA+GKPANPFAFGSGHVDPEKASDPGL+YDITP+DYLNYLCSLNYNSTQI LVSRG
Sbjct: 601 SDVGSASGKPANPFAFGSGHVDPEKASDPGLIYDITPQDYLNYLCSLNYNSTQIGLVSRG 660

Query: 661 SFTCPSKRTFLQPGDLNYPSFSVFMKKKAKNVTVTLKRTVTNVGSPMSDYTVQINNPKGI 720
           +FTCPSKR   Q G LNYPSFSVFMKKKAKNV+VTLKRTVTNVG P SDY+V+I NPKGI
Sbjct: 661 NFTCPSKRRVGQAGKLNYPSFSVFMKKKAKNVSVTLKRTVTNVGRPRSDYSVKIKNPKGI 720

Query: 721 KISVKPEKLSFGRLGEKLSYRVSFVAFGKEEALSEFSFGSLVWLSGKYVVKSPIAVTWQ 777
            ISVKPEKLSF R G+KLSY+VSFVA GK E +S FSFGSLVW+SG Y V+SPIAVTW+
Sbjct: 721 GISVKPEKLSFRRYGQKLSYQVSFVALGKREGVSGFSFGSLVWVSGTYAVRSPIAVTWK 764

BLAST of Lag0041267 vs. NCBI nr
Match: XP_023544740.1 (subtilisin-like protease SBT1.1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1190.6 bits (3079), Expect = 0.0e+00
Identity = 594/779 (76.25%), Postives = 674/779 (86.52%), Query Frame = 0

Query: 1   MAFREVWVLLCIVFAASTAALDQQTYIIHMDTTKMPTTNPQQWYTSMIDSVNELASLDED 60
           M FREV + L I  A STAA+DQQ+YIIHMDTTKM    P+QWYT++IDS+NE++SL  D
Sbjct: 1   MGFREVCLFLSIFLATSTAAVDQQSYIIHMDTTKMAAPTPEQWYTALIDSINEISSL-HD 60

Query: 61  EEEVSNAAEIFYVYKNAISGKELKCVLDKYKNNGFAAKLSTKKLNSLSQTPGFLTAIPDQ 120
           ++E SNAA+I YVYK AIS              GFAAKLSTKKL+SLS+TPGFL A P++
Sbjct: 61  QQEASNAAQILYVYKTAIS--------------GFAAKLSTKKLHSLSKTPGFLAATPNE 120

Query: 121 LLQLHTTHSPHFLGLKTGHGLWNATNLASDVIVGVVDTGIWPEHVSFEDKGLPPVPRRWK 180
           LLQLHTTHSP FLGL+  HGLWN++NLASD+++G++DTGIWPEH+SF+DKGLPPVP++WK
Sbjct: 121 LLQLHTTHSPQFLGLQRQHGLWNSSNLASDIVIGLLDTGIWPEHISFQDKGLPPVPKKWK 180

Query: 181 GGCEEGQKFSASNCNRKLIGARAFIKAYEASFGRLNESGVFRSPRDWNGHGTHTASTAAG 240
           G C+ GQKFS SNCNRKLIGA A+IK YEA  GRLN +G FRSPRD +GHGTHTASTAAG
Sbjct: 181 GTCQPGQKFSPSNCNRKLIGAMAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAG 240

Query: 241 NLVFKAGFYNHALGNAAGIRFTSRIAAYKVCWPLGCAIADVLAAMDRAVADGVDVLSLSL 300
           N+V KA F+N A+G A G+RFTSRIAAYKVCW  GCA AD+LAA+DRAVADGVDVLSLSL
Sbjct: 241 NIVNKASFFNQAMGVATGMRFTSRIAAYKVCWTEGCANADILAAIDRAVADGVDVLSLSL 300

Query: 301 GGNPVPFYNDGLAIAAFGATQKGVFVSCAAGNSGPAPSTAGNLAPWIMTVGASYTDRIFP 360
           GG+   FY D +AIA FGA + GVFVSC+AGNSGP+ ST  N+APWIMTV ASYTDR FP
Sbjct: 301 GGSASAFYKDDIAIATFGAVRNGVFVSCSAGNSGPSSSTVSNIAPWIMTVAASYTDRTFP 360

Query: 361 TTVKLGNGQVFEGSSLFSGNNMGQLPLVYKQ--GRQGANLCLAGSLVPSMVRGKIVICER 420
            TVKLGNGQVFEGSSL++GNN+GQLPLVY    G Q AN+C  GSLVPSMV+GKIV+CER
Sbjct: 361 ATVKLGNGQVFEGSSLYTGNNIGQLPLVYNNTAGGQEANVCTPGSLVPSMVKGKIVVCER 420

Query: 421 GTNTRIGKGEQVKLAGGAGMILINTQLEGEELFADPHVLPATSLGAAAGKAIKNYIASSK 480
           GTN+R  KGEQVKLAGGAGMILINTQLEGEELFADPHVLPA +LGA+AG+AI  YI+SSK
Sbjct: 421 GTNSRTAKGEQVKLAGGAGMILINTQLEGEELFADPHVLPAANLGASAGQAIIRYISSSK 480

Query: 481 -QPKASIAFQGTKYGSRAPRVAAFSSRGPSSIEPNIIKPDIIAPGVNILAAWPSIVSPSE 540
            QPKA I F+GTK+G+RAPRVAAFSSRGPS I P++IKPD+ APGVNILAAWP I SPSE
Sbjct: 481 HQPKALIGFEGTKFGNRAPRVAAFSSRGPSLIAPDVIKPDVTAPGVNILAAWPLIASPSE 540

Query: 541 LEFDKRRVLFNIVSGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTNDNKMSPI 600
           LE DKRRVLFN++SGTSMSCPHVSGLAALLK+AHKDWSPAAIKSALMTTAYTNDN+MSPI
Sbjct: 541 LESDKRRVLFNVISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNRMSPI 600

Query: 601 SDVGSANGKPANPFAFGSGHVDPEKASDPGLVYDITPEDYLNYLCSLNYNSTQIALVSRG 660
           SDVGSA+GKPANPFAFGSGHVDPEKASDPGL+YDITP+DYL+Y CSLNYNSTQI LVSRG
Sbjct: 601 SDVGSASGKPANPFAFGSGHVDPEKASDPGLIYDITPQDYLDYFCSLNYNSTQIGLVSRG 660

Query: 661 SFTCPSKRTFLQPGDLNYPSFSVFMKKKAKNVTVTLKRTVTNVGSPMSDYTVQINNPKGI 720
           +FTCPSKR   QPG+LNYPSFSVFMKKKAKNV+VTLKRTVTNVG P SDY+V+I NPKGI
Sbjct: 661 NFTCPSKRRVGQPGNLNYPSFSVFMKKKAKNVSVTLKRTVTNVGGPRSDYSVKIKNPKGI 720

Query: 721 KISVKPEKLSFGRLGEKLSYRVSFVAFGKEEALSEFSFGSLVWLSGKYVVKSPIAVTWQ 777
            ISVKPEKLSF R G+KLSY+VSFVA GK E +S FSFGSLVW+SGKY V+SPIAVTW+
Sbjct: 721 VISVKPEKLSFRRYGQKLSYQVSFVALGKREGVSGFSFGSLVWVSGKYAVRSPIAVTWK 764

BLAST of Lag0041267 vs. NCBI nr
Match: XP_022990941.1 (subtilisin-like protease SBT1.1 [Cucurbita maxima] >XP_022990948.1 subtilisin-like protease SBT1.1 [Cucurbita maxima])

HSP 1 Score: 1186.4 bits (3068), Expect = 0.0e+00
Identity = 593/779 (76.12%), Postives = 674/779 (86.52%), Query Frame = 0

Query: 1   MAFREVWVLLCIVFAASTAALDQQTYIIHMDTTKMPTTNPQQWYTSMIDSVNELASLDED 60
           M FREV + L I  A S AA+DQQ+YIIHMDTTKM   +P+QWYT++IDS+N+++SLD D
Sbjct: 1   MGFREVCLFLSIFLATSAAAVDQQSYIIHMDTTKMAAPSPEQWYTALIDSINKISSLD-D 60

Query: 61  EEEVSNAAEIFYVYKNAISGKELKCVLDKYKNNGFAAKLSTKKLNSLSQTPGFLTAIPDQ 120
           +EE S+AA+I YVYK AIS              GFAAKLSTKKL+SLS+TPGFL A P++
Sbjct: 61  QEEASSAAQILYVYKTAIS--------------GFAAKLSTKKLHSLSKTPGFLAATPNE 120

Query: 121 LLQLHTTHSPHFLGLKTGHGLWNATNLASDVIVGVVDTGIWPEHVSFEDKGLPPVPRRWK 180
           LLQLHTTHSP FLGL+  HGLWN++NLASD+++G++DTGIWPEH+SF+DKGLPPVP++WK
Sbjct: 121 LLQLHTTHSPQFLGLQREHGLWNSSNLASDIVIGLLDTGIWPEHISFQDKGLPPVPKKWK 180

Query: 181 GGCEEGQKFSASNCNRKLIGARAFIKAYEASFGRLNESGVFRSPRDWNGHGTHTASTAAG 240
           G C+ GQKFS SNCNRKLIGA A+IK YEA  GRLN +G FRSPRD +GHGTHTASTAAG
Sbjct: 181 GTCQAGQKFSPSNCNRKLIGATAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAG 240

Query: 241 NLVFKAGFYNHALGNAAGIRFTSRIAAYKVCWPLGCAIADVLAAMDRAVADGVDVLSLSL 300
           N+V KA F+N A+G A G+RFTSRIAAYKVCW  GCA AD+LAA+DRAVADGVDVLSLSL
Sbjct: 241 NIVNKASFFNQAMGVATGMRFTSRIAAYKVCWTEGCANADILAAIDRAVADGVDVLSLSL 300

Query: 301 GGNPVPFYNDGLAIAAFGATQKGVFVSCAAGNSGPAPSTAGNLAPWIMTVGASYTDRIFP 360
           GG+   FY D +AIA FGA + GVFVSC+AGNSGP+ ST  N+APWIMTV ASYTDR FP
Sbjct: 301 GGSASAFYKDDIAIATFGAVRNGVFVSCSAGNSGPSSSTVSNIAPWIMTVAASYTDRTFP 360

Query: 361 TTVKLGNGQVFEGSSLFSGNNMGQLPLVYKQ--GRQGANLCLAGSLVPSMVRGKIVICER 420
            TVKLGNGQVFEGSSL+SGN++GQLPLVY    G + AN+C AGSLVPS+V+GKIV+CER
Sbjct: 361 ATVKLGNGQVFEGSSLYSGNSIGQLPLVYNNTAGGEEANVCTAGSLVPSLVKGKIVVCER 420

Query: 421 GTNTRIGKGEQVKLAGGAGMILINTQLEGEELFADPHVLPATSLGAAAGKAIKNYIASSK 480
           GTN+R  KGEQVKLAGGAGMILINTQLEGEELFADPHVLPA +LGA+AG+AI  YI+SSK
Sbjct: 421 GTNSRTAKGEQVKLAGGAGMILINTQLEGEELFADPHVLPAANLGASAGQAIIRYISSSK 480

Query: 481 -QPKASIAFQGTKYGSRAPRVAAFSSRGPSSIEPNIIKPDIIAPGVNILAAWPSIVSPSE 540
            QPKA IAF+GTK+G+RAPRVAAFSSRGPS I P++IKPD+ APGVNILAAWP I SPSE
Sbjct: 481 HQPKALIAFEGTKFGNRAPRVAAFSSRGPSLIAPDVIKPDVTAPGVNILAAWPLIASPSE 540

Query: 541 LEFDKRRVLFNIVSGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTNDNKMSPI 600
           LE DKRRVLFNI+SGTSMSCPHVSGLAALLK+AHKDWSPAAIKSALMTTAYTNDN+MSPI
Sbjct: 541 LESDKRRVLFNIISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNRMSPI 600

Query: 601 SDVGSANGKPANPFAFGSGHVDPEKASDPGLVYDITPEDYLNYLCSLNYNSTQIALVSRG 660
           SDVGS +GKPANPFAFGSGHVDPEKASDPGL+YDITP+DYLNY CSLNYNSTQI LVSRG
Sbjct: 601 SDVGSESGKPANPFAFGSGHVDPEKASDPGLIYDITPQDYLNYFCSLNYNSTQIGLVSRG 660

Query: 661 SFTCPSKRTFLQPGDLNYPSFSVFMKKKAKNVTVTLKRTVTNVGSPMSDYTVQINNPKGI 720
           +FTCPSKR   QPG+LNYPSFSVFMKKKAKNV+VTLKRTVTNVG P SDYTV+I NPKGI
Sbjct: 661 NFTCPSKRRVGQPGNLNYPSFSVFMKKKAKNVSVTLKRTVTNVGRPRSDYTVKIKNPKGI 720

Query: 721 KISVKPEKLSFGRLGEKLSYRVSFVAFGKEEALSEFSFGSLVWLSGKYVVKSPIAVTWQ 777
            I V+PEKLSF R G+KLSY+VSFVA GK E L  FSFGSLVW+SGKY V+SPIAVTW+
Sbjct: 721 GIRVEPEKLSFRRYGQKLSYQVSFVALGKREGLGGFSFGSLVWVSGKYGVRSPIAVTWK 764

BLAST of Lag0041267 vs. NCBI nr
Match: XP_038893069.1 (subtilisin-like protease SBT1.1 [Benincasa hispida] >XP_038893070.1 subtilisin-like protease SBT1.1 [Benincasa hispida])

HSP 1 Score: 1173.3 bits (3034), Expect = 0.0e+00
Identity = 591/781 (75.67%), Postives = 667/781 (85.40%), Query Frame = 0

Query: 1   MAFREVWVLLCIVFAASTAALDQQTYIIHMDTTKMPTTNPQQWYTSMIDSVNELASL-DE 60
           M FREVW+ L I+ A +TAA+DQQTYIIHMDTTKM TTNP+QWYT+MIDS+NEL SL DE
Sbjct: 1   MGFREVWMFLSIMLAIATAAVDQQTYIIHMDTTKMTTTNPEQWYTAMIDSLNELPSLDDE 60

Query: 61  DEEEVSNAAEIFYVYKNAISGKELKCVLDKYKNNGFAAKLSTKKLNSLSQTPGFLTAIPD 120
           ++EE S+ AEI YVYK A+S              GFAAKLS KKL+SLS+ PGFL A P+
Sbjct: 61  NKEEASDTAEILYVYKTALS--------------GFAAKLSRKKLDSLSKIPGFLAATPN 120

Query: 121 QLLQLHTTHSPHFLGLKTGHGLWNATNLASDVIVGVVDTGIWPEHVSFEDKGLPPVPRRW 180
           +LLQLHTTHSP FLGL+  HGLWN++NLASD+I+G++DTGIWPEH+SF+DKGL PVP +W
Sbjct: 121 ELLQLHTTHSPQFLGLERDHGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLSPVPSKW 180

Query: 181 KGGCEEGQKFSASNCNRKLIGARAFIKAYEASFGRLNESGVFRSPRDWNGHGTHTASTAA 240
           KG C+ G KFS SNCN+KLIGA A+IK YEA  G LNE+G FRSPRD +GHGTHTASTAA
Sbjct: 181 KGICQAGPKFSPSNCNKKLIGASAYIKGYEAIVGTLNETGTFRSPRDSDGHGTHTASTAA 240

Query: 241 GNLVFKAGFYNHALGNAAGIRFTSRIAAYKVCWPLGCAIADVLAAMDRAVADGVDVLSLS 300
           G++V KA F+N  +G A G+ +TSRIAAYKVCWPLGCA AD+LAAMD AVADGVDVLSLS
Sbjct: 241 GSMVNKASFFNQGMGVATGMMYTSRIAAYKVCWPLGCANADILAAMDSAVADGVDVLSLS 300

Query: 301 LGGNPVPFYNDGLAIAAFGATQKGVFVSCAAGNSGPAPSTAGNLAPWIMTVGASYTDRIF 360
           LGG    FY D +AIAAFGA Q GVFVSC+AGNSGP  ST GN APWIMTV ASYTDR F
Sbjct: 301 LGGGAGSFYRDNIAIAAFGAIQNGVFVSCSAGNSGPFISTVGNAAPWIMTVAASYTDRTF 360

Query: 361 PTTVKLGNGQVFEGSSLFSGNNMGQLPLVYKQ---GRQGANLCLAGSLVPSMVRGKIVIC 420
           PTTVKLGNGQVFEGSSL+ G N+  LPLVY       Q  N C AGSL P+MV+GKIV+C
Sbjct: 361 PTTVKLGNGQVFEGSSLYYGKNINYLPLVYNNTAGDGQETNFCTAGSLDPTMVKGKIVLC 420

Query: 421 ERGTNTRIGKGEQVKLAGGAGMILINTQLEGEELFADPHVLPATSLGAAAGKAIKNYIAS 480
           ERG+N+R  KGEQVKLAGGAGMILINTQ EGEELFADPHVLPAT+LGA+AGKAI +YIAS
Sbjct: 421 ERGSNSRTDKGEQVKLAGGAGMILINTQFEGEELFADPHVLPATTLGASAGKAILDYIAS 480

Query: 481 SK-QPKASIAFQGTKYGSRAPRVAAFSSRGPSSIEPNIIKPDIIAPGVNILAAWPSIVSP 540
           SK Q KAS+AF+GTKYGSRAPRVAAFSSRGPS + P+++KPD+ APGVNILAAWP IVSP
Sbjct: 481 SKSQAKASMAFEGTKYGSRAPRVAAFSSRGPSFVGPDVMKPDVTAPGVNILAAWPPIVSP 540

Query: 541 SELEFDKRRVLFNIVSGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTNDNKMS 600
           SEL+ DKRRVLFNI+SGTSMSCPHVSGLAALLK+AHKDWSPAAIKSALMTTAY  DNKM+
Sbjct: 541 SELDSDKRRVLFNIISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVTDNKMN 600

Query: 601 PISDVGSANGKPANPFAFGSGHVDPEKASDPGLVYDITPEDYLNYLCSLNYNSTQIALVS 660
            +SDVG  +G PA+PFAFGSGHVDPEKASDPGLVYDITP+DY+NYLCSL YNSTQIALVS
Sbjct: 601 LVSDVGHPSGGPADPFAFGSGHVDPEKASDPGLVYDITPQDYINYLCSLKYNSTQIALVS 660

Query: 661 RGSFTCPSKRTFLQPGDLNYPSFSVFMKKKAKNVTVTLKRTVTNVGSPMSDYTVQINNPK 720
           RG+FTC SKRT LQP DLNYPSFSVFMKKKAKNV++T KRTVTNVG P SDYTV+INNPK
Sbjct: 661 RGNFTCSSKRTLLQPRDLNYPSFSVFMKKKAKNVSITSKRTVTNVGIPRSDYTVKINNPK 720

Query: 721 GIKISVKPEKLSFGRLGEKLSYRVSFVAFGKEEALSEFSFGSLVWLSGKYVVKSPIAVTW 777
           GI+I+VKPEKLSFG LGEKLS++VSFVA G +EALS+FSFG LVWLSGKY V+SPIAVTW
Sbjct: 721 GIRITVKPEKLSFGSLGEKLSFQVSFVALGGKEALSKFSFGDLVWLSGKYAVRSPIAVTW 767

BLAST of Lag0041267 vs. NCBI nr
Match: XP_022150014.1 (subtilisin-like protease SBT1.1 [Momordica charantia] >XP_022150015.1 subtilisin-like protease SBT1.1 [Momordica charantia] >XP_022150016.1 subtilisin-like protease SBT1.1 [Momordica charantia])

HSP 1 Score: 1162.9 bits (3007), Expect = 0.0e+00
Identity = 583/782 (74.55%), Postives = 670/782 (85.68%), Query Frame = 0

Query: 1   MAFREVWVLLCIVFAAST--AALDQQTYIIHMDTTKMPTTNPQQWYTSMIDSVNELASLD 60
           M  REVWV+L I+ A S+  AA+DQ+T+IIHMDTTKMP T+P+QWYTSMI SVNEL SL+
Sbjct: 1   MGLREVWVVLSIMVATSSAAAAVDQRTFIIHMDTTKMPATDPEQWYTSMIHSVNELPSLN 60

Query: 61  EDEEEVSNAAEIFYVYKNAISGKELKCVLDKYKNNGFAAKLSTKKLNSLSQTPGFLTAIP 120
            DE E SN AE+ YVYK A+S               FAAKL+T+KL+++S+  GF+ AIP
Sbjct: 61  TDEGEASNTAEVLYVYKTALS--------------XFAAKLTTRKLDAISKIRGFVAAIP 120

Query: 121 DQLLQLHTTHSPHFLGLKTGHGLWNATNLASDVIVGVVDTGIWPEHVSFEDKGLPPVPRR 180
           D+LLQLHTTHS +FLGL+  HGLWN++ LASD+IVGV+DTGIWPEHVSF DKG+P VPRR
Sbjct: 121 DELLQLHTTHSSNFLGLELDHGLWNSSTLASDIIVGVLDTGIWPEHVSFHDKGVPRVPRR 180

Query: 181 WKGGCEEGQKFSASNCNRKLIGARAFIKAYEASFGRLNESGVFRSPRDWNGHGTHTASTA 240
           WKG CE+G KFS SNCNRKLIGA AF+K YE+  GRLN++  +RSPRD +GHGTHTASTA
Sbjct: 181 WKGVCEQGPKFSPSNCNRKLIGAAAFLKGYESIVGRLNQTATYRSPRDSDGHGTHTASTA 240

Query: 241 AGNLVFKAGFYNHALGNAAGIRFTSRIAAYKVCWPLGCAIADVLAAMDRAVADGVDVLSL 300
           AGNLV+KAGFY+ A+G AAG+RFTSRIAAYKVCWP GCA AD+LAA+DRAVADGVDVLSL
Sbjct: 241 AGNLVYKAGFYDQAMGAAAGMRFTSRIAAYKVCWPAGCANADILAAIDRAVADGVDVLSL 300

Query: 301 SLGGNPVPFYNDGLAIAAFGATQKGVFVSCAAGNSGPAPSTAGNLAPWIMTVGASYTDRI 360
           SLGG   PFY DG++IAAFGA + GVFVSC+AGNSGP+PST  N+APWIMTV ASYTDR 
Sbjct: 301 SLGGRAAPFYRDGISIAAFGAVRNGVFVSCSAGNSGPSPSTVSNVAPWIMTVAASYTDRT 360

Query: 361 FPTTVKLGNGQVFEGSSLFSGNNMGQLPLVYKQ--GRQGANLCLAGSLVPSMVRGKIVIC 420
           FPTTVKLG+GQVF+GSSL+SG N  QLPLVY +  G Q ANLC  GSL+P+MV+GKIV+C
Sbjct: 361 FPTTVKLGSGQVFQGSSLYSGKNTNQLPLVYNKTAGPQEANLCTPGSLIPAMVKGKIVVC 420

Query: 421 ERGTNTRIGKGEQVKLAGGAGMILINTQLEGEELFADPHVLPATSLGAAAGKAIKNYIAS 480
           ERGT +R  KGEQVKLAGGA MILINT+LEGEELFADPHVLPATSLGA+AGKAIK +IAS
Sbjct: 421 ERGTISRTAKGEQVKLAGGAAMILINTELEGEELFADPHVLPATSLGASAGKAIKKFIAS 480

Query: 481 SK-QPKASIAFQGTKYGSRAPRVAAFSSRGPSSIEPNIIKPDIIAPGVNILAAWPSIVSP 540
           SK QPK SI F GT+YGSRAPRVAAFSSRGP+ I+  I+KPD+ APGVNILAAWP+I+SP
Sbjct: 481 SKDQPKGSITFGGTRYGSRAPRVAAFSSRGPNPIQRTIMKPDVTAPGVNILAAWPAIISP 540

Query: 541 SELEFDKRRVLFNIVSGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTNDNKMS 600
           SELE DKRRVLFNI+SGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYT DNKM 
Sbjct: 541 SELESDKRRVLFNIISGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTTDNKMR 600

Query: 601 PISDVGSANGKPANPFAFGSGHVDPEKASDPGLVYDITPEDYLNYLCSLNYNSTQIALVS 660
            ISDVGS +G+PAN FAFGSGHVDPEKASDPGLVYDITP DYLNYLCSLNY S+QIAL+S
Sbjct: 601 LISDVGSTSGRPANAFAFGSGHVDPEKASDPGLVYDITPRDYLNYLCSLNYTSSQIALLS 660

Query: 661 RGSFTCPSKRTFLQPGDLNYPSFSVFM-KKKAKNVTVTLKRTVTNVGSPMSDYTVQINNP 720
           R +F+CPS+  FL+PGDLNYPSFSV M +KKA NV+VTLKRTVTNVGSP  DYTV+IN+P
Sbjct: 661 RENFSCPSRGAFLKPGDLNYPSFSVIMDQKKATNVSVTLKRTVTNVGSPRGDYTVKINSP 720

Query: 721 KGIKISVKPEKLSFGRLGEKLSYRVSFVAFGKEEALSEFSFGSLVWLSGKYVVKSPIAVT 777
           KG+ I VKP+KLSF RLGEKLSY+VSF++ GK ++  + SFGSLVWLSGKY V+SP+AVT
Sbjct: 721 KGVAIIVKPQKLSFKRLGEKLSYKVSFISLGKGKSFGDSSFGSLVWLSGKYSVRSPVAVT 768

BLAST of Lag0041267 vs. ExPASy Swiss-Prot
Match: Q84WS0 (Subtilisin-like protease SBT1.1 OS=Arabidopsis thaliana OX=3702 GN=SBTI1.1 PE=1 SV=1)

HSP 1 Score: 808.5 bits (2087), Expect = 6.5e-233
Identity = 423/780 (54.23%), Postives = 541/780 (69.36%), Query Frame = 0

Query: 1   MAFREVWVLLCIVFAASTAALDQQTYIIHMDTTKMPTTNPQQWYTSMIDSVNELASLDED 60
           M FR   V   ++F AS  +  +QTY+IH       TT+ +   TS+ +S+ +  ++++D
Sbjct: 19  MFFRSFIVFFFLIFFASNVSSRKQTYVIH-----TVTTSTKHIVTSLFNSL-QTENINDD 78

Query: 61  EEEVSNAAEIFYVYKNAISGKELKCVLDKYKNNGFAAKLSTKKLNSLSQTPGFLTAIPDQ 120
           +    +  EI Y+Y+NA+S              GF+A L+  +L+++  T GF++A PD+
Sbjct: 79  D---FSLPEIHYIYENAMS--------------GFSATLTDDQLDTVKNTKGFISAYPDE 138

Query: 121 LLQLHTTHSPHFLGLKTGHGLWNATNLASDVIVGVVDTGIWPEHVSFEDKGLPPVPRRWK 180
           LL LHTT+S  FLGL+ G GLWN T+L+SDVI+G+VDTGI PEHVSF D  + PVP RW+
Sbjct: 139 LLSLHTTYSHEFLGLEFGIGLWNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWR 198

Query: 181 GGCEEGQKFSASNCNRKLIGARAFIKAYEASFGRLNESGVFRSPRDWNGHGTHTASTAAG 240
           G C+EG  FS+S CN+K+IGA AF K YE+  G++NE+  FRS RD  GHGTHTASTAAG
Sbjct: 199 GSCDEGTNFSSSECNKKIIGASAFYKGYESIVGKINETTDFRSTRDAQGHGTHTASTAAG 258

Query: 241 NLVFKAGFYNHALGNAAGIRFTSRIAAYKVCWPLGCAIADVLAAMDRAVADGVDVLSLSL 300
           ++V KA ++  A G A+G+RFTSRIAAYK CW LGCA  DV+AA+DRA+ DGVDV+SLSL
Sbjct: 259 DIVPKANYFGQAKGLASGMRFTSRIAAYKACWALGCASTDVIAAIDRAILDGVDVISLSL 318

Query: 301 GGNPVPFYNDGLAIAAFGATQKGVFVSCAAGNSGPAPSTAGNLAPWIMTVGASYTDRIFP 360
           GG+  PFY D +AIA FGA QK +FVSC+AGNSGP  ST  N APW+MTV ASYTDR FP
Sbjct: 319 GGSSRPFYVDPIAIAGFGAMQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFP 378

Query: 361 TTVKLGNGQVFEGSSLFSGNNMGQLPLVYKQ---GRQGANLCLAGSLVPSMVRGKIVICE 420
             V++GN +   GSSL+ G ++  LPL + +      GA  C+  SL   +V GKIVIC 
Sbjct: 379 AIVRIGNRKSLVGSSLYKGKSLKNLPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVICL 438

Query: 421 RGTNTRIGKGEQVKLAGGAGMILINTQLEGEELFADPHVLPATSLGAAAGKAIKNYIASS 480
           RG + R  KGE+VK +GGA M+L++T+ EGEEL ADPHVLPA SLG + GK + NY+A +
Sbjct: 439 RGASGRTAKGEEVKRSGGAAMLLVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLAGA 498

Query: 481 KQPKASIAFQGTKYGSRAPRVAAFSSRGPSSIEPNIIKPDIIAPGVNILAAWPSIVSPSE 540
               AS+ F+GT YG+ AP VAAFSSRGPS   P I KPDI APG+NILA W    SPS 
Sbjct: 499 ANATASVRFRGTAYGATAPMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSL 558

Query: 541 LEFDKRRVLFNIVSGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTNDNKMSPI 600
           L  D RRV FNI+SGTSM+CPH+SG+AAL+K+ H DWSPA IKSA+MTTA   DN+  PI
Sbjct: 559 LRSDPRRVQFNIISGTSMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPI 618

Query: 601 SDVGSANGK-PANPFAFGSGHVDPEKASDPGLVYDITPEDYLNYLCSLNYNSTQIALVSR 660
            D G+A  +  A  FAFG+G+VDP +A DPGLVYD +  DYLNYLCSLNY S +I L S 
Sbjct: 619 GDRGAAGAESAATAFAFGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSG 678

Query: 661 GSFTCPSKRTFLQPGDLNYPSFSVFMKKKAKNVTVTLKRTVTNVGSPMSDYTVQINNPKG 720
            ++TC S    L PGDLNYPSF+V +   A   TV  KRTVTNVGSP  +Y V +  PKG
Sbjct: 679 TNYTCASNAVVLSPGDLNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKG 738

Query: 721 IKISVKPEKLSFGRLGEKLSYRVSFVAFGKEEALSEFSFGSLVWLSGKYVVKSPIAVTWQ 777
           +K+ V+P+ L F +  E+LSY V++ A     + S  SFG LVW+  KY V+SPIAVTW+
Sbjct: 739 VKVRVEPKVLKFQKARERLSYTVTYDAEASRNS-SSSSFGVLVWICDKYNVRSPIAVTWE 774

BLAST of Lag0041267 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 704.5 bits (1817), Expect = 1.3e-201
Identity = 382/780 (48.97%), Postives = 513/780 (65.77%), Query Frame = 0

Query: 7   WVLLCIVFA-ASTAALDQQTYIIHMDTTKMPTTNP--QQWYTSMIDSVNELASLDEDEEE 66
           ++LLC+ F   S+++ DQ TYI+HM  ++MP++      WY S + S             
Sbjct: 12  FLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRS------------- 71

Query: 67  VSNAAEIFYVYKNAISGKELKCVLDKYKNNGFAAKLSTKKLNSLSQTPGFLTAIPDQLLQ 126
           +S++AE+ Y Y+NAI              +GF+ +L+ ++ +SL   PG ++ +P+   +
Sbjct: 72  ISDSAELLYTYENAI--------------HGFSTRLTQEEADSLMTQPGVISVLPEHRYE 131

Query: 127 LHTTHSPHFLGL-KTGHGLWNATNLASDVIVGVVDTGIWPEHVSFEDKGLPPVPRRWKGG 186
           LHTT +P FLGL +    L+      SDV+VGV+DTG+WPE  S+ D+G  P+P  WKGG
Sbjct: 132 LHTTRTPLFLGLDEHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGG 191

Query: 187 CEEGQKFSASNCNRKLIGARAFIKAYEASFGRLNESGVFRSPRDWNGHGTHTASTAAGNL 246
           CE G  F+AS CNRKLIGAR F + YE++ G ++ES   RSPRD +GHGTHT+STAAG++
Sbjct: 192 CEAGTNFTASLCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSV 251

Query: 247 VFKAGFYNHALGNAAGIRFTSRIAAYKVCWPLGCAIADVLAAMDRAVADGVDVLSLSLGG 306
           V  A    +A G A G+   +R+A YKVCW  GC  +D+LAA+D+A+AD V+VLS+SLGG
Sbjct: 252 VEGASLLGYASGTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGG 311

Query: 307 NPVPFYNDGLAIAAFGATQKGVFVSCAAGNSGPAPSTAGNLAPWIMTVGASYTDRIFPTT 366
               +Y DG+AI AF A ++G+ VSC+AGN+GP+ S+  N+APWI TVGA   DR FP  
Sbjct: 312 GMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPAL 371

Query: 367 VKLGNGQVFEGSSLFSGNNMGQ--LPLVYKQGRQGA---NLCLAGSLVPSMVRGKIVICE 426
             LGNG+ F G SLF G  +    LP +Y      A   NLC+ G+L+P  V+GKIV+C+
Sbjct: 372 AILGNGKNFTGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCD 431

Query: 427 RGTNTRIGKGEQVKLAGGAGMILINTQLEGEELFADPHVLPATSLGAAAGKAIKNYIASS 486
           RG N R+ KG+ VK AGG GMIL NT   GEEL AD H+LPAT++G  AG  I++Y+ + 
Sbjct: 432 RGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTD 491

Query: 487 KQPKASIAFQGTKYGSR-APRVAAFSSRGPSSIEPNIIKPDIIAPGVNILAAWPSIVSPS 546
             P ASI+  GT  G + +P VAAFSSRGP+SI PNI+KPD+IAPGVNILAAW     P+
Sbjct: 492 PNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPT 551

Query: 547 ELEFDKRRVLFNIVSGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTNDNKMSP 606
            L  D RRV FNI+SGTSMSCPHVSGLAALLK+ H +WSPAAI+SALMTTAY       P
Sbjct: 552 GLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKP 611

Query: 607 ISDVGSANGKPANPFAFGSGHVDPEKASDPGLVYDITPEDYLNYLCSLNYNSTQIALVSR 666
           + D+  A GKP+ PF  G+GHV P  A++PGL+YD+T EDYL +LC+LNY S QI  VSR
Sbjct: 612 LLDI--ATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSR 671

Query: 667 GSFTCPSKRTFLQPGDLNYPSFSVFMKKKAKNVTVTLKRTVTNVGSPMSDYTVQI-NNPK 726
            ++TC   +++    DLNYPSF+V +            RTVT+VG     Y+V++ +   
Sbjct: 672 RNYTCDPSKSY-SVADLNYPSFAVNVDGVG---AYKYTRTVTSVGG-AGTYSVKVTSETT 731

Query: 727 GIKISVKPEKLSFGRLGEKLSYRVSFVAFGKEEALSEFSFGSLVWLSGKYVVKSPIAVTW 776
           G+KISV+P  L+F    EK SY V+F     + + S  SFGS+ W  GK+VV SP+A++W
Sbjct: 732 GVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSN-SFGSIEWSDGKHVVGSPVAISW 756

BLAST of Lag0041267 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 3.4e-197
Identity = 382/784 (48.72%), Postives = 504/784 (64.29%), Query Frame = 0

Query: 8   VLLCIVFAASTAALDQQTYIIHMDTTKMPT--TNPQQWYTSMIDSVNELASLDEDEEEVS 67
           +LLC    +S+++   ++YI+H+  +  P+  ++   W+ S++ S+              
Sbjct: 15  LLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSL----------PSSP 74

Query: 68  NAAEIFYVYKNAISGKELKCVLDKYKNNGFAAKLSTKKLNSLSQTPGFLTAIPDQLLQLH 127
             A + Y Y  A+              +GF+A+LS  +  +L + P  ++ IPDQ  ++H
Sbjct: 75  QPATLLYSYSRAV--------------HGFSARLSPIQTAALRRHPSVISVIPDQAREIH 134

Query: 128 TTHSPHFLGLKTGHGLWNATNLASDVIVGVVDTGIWPEHVSFEDKGLPPVPRRWKGGCEE 187
           TTH+P FLG     GLW+ +N   DVIVGV+DTGIWPEH SF D GL P+P  WKG CE 
Sbjct: 135 TTHTPAFLGFSQNSGLWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEI 194

Query: 188 GQKFSASNCNRKLIGARAFIKAY--EASFGRLNESGVFRSPRDWNGHGTHTASTAAGNLV 247
           G  F AS+CNRKLIGARAF + Y  + +  + + +   RSPRD  GHGTHTASTAAG++V
Sbjct: 195 GPDFPASSCNRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVV 254

Query: 248 FKAGFYNHALGNAAGIRFTSRIAAYKVCWPLGCAIADVLAAMDRAVADGVDVLSLSLG-- 307
             A  Y +A G A G+   +RIAAYK+CW  GC  +D+LAAMD+AVADGV V+SLS+G  
Sbjct: 255 ANASLYQYARGTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGAS 314

Query: 308 GNPVPFYNDGLAIAAFGATQKGVFVSCAAGNSGPAPSTAGNLAPWIMTVGASYTDRIFPT 367
           G+   ++ D +AI AFGAT+ G+ VSC+AGNSGP P TA N+APWI+TVGAS  DR F  
Sbjct: 315 GSAPEYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAA 374

Query: 368 TVKLGNGQVFEGSSLFSGNNM--GQLPLVYKQGRQGANLCLAGSLVPSMVRGKIVICERG 427
               G+G+VF G+SL++G ++   QL LVY  G  G+ LC  G L  S+V GKIV+C+RG
Sbjct: 375 NAITGDGKVFTGTSLYAGESLPDSQLSLVY-SGDCGSRLCYPGKLNSSLVEGKIVLCDRG 434

Query: 428 TNTRIGKGEQVKLAGGAGMILINTQLEGEELFADPHVLPATSLGAAAGKAIKNYIASSKQ 487
            N R+ KG  VKLAGGAGMIL NT   GEEL AD H++PAT +GA AG  I++YI +S  
Sbjct: 435 GNARVEKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDS 494

Query: 488 PKASIAFQGTKYGSR--APRVAAFSSRGPSSIEPNIIKPDIIAPGVNILAAWPSIVSPSE 547
           P A I+F GT  G    +PRVAAFSSRGP+ + P I+KPD+IAPGVNILA W  +V P++
Sbjct: 495 PTAKISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTD 554

Query: 548 LEFDKRRVLFNIVSGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTNDNKMSPI 607
           L+ D RRV FNI+SGTSMSCPHVSGLAALL+ AH DWSPAAIKSAL+TTAY  +N   PI
Sbjct: 555 LDIDPRRVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPI 614

Query: 608 SDVGSANGKPANPFAFGSGHVDPEKASDPGLVYDITPEDYLNYLCSLNYNSTQIALVSRG 667
            D+  A GK +N F  G+GHVDP KA +PGLVYDI  ++Y+ +LC++ Y    I +  + 
Sbjct: 615 EDL--ATGKSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQD 674

Query: 668 SFTCPSKRT--FLQPGDLNYPSFSVFMKKKAKNVTVTLKRTVTNVGSPM-SDYTVQINNP 727
                +  T      GDLNYPSFSV      +   V  KR V NVGS + + Y V + +P
Sbjct: 675 PTLYDACETSKLRTAGDLNYPSFSVVFASTGE--VVKYKRVVKNVGSNVDAVYEVGVKSP 734

Query: 728 KGIKISVKPEKLSFGRLGEKLSYRVSF---VAFGKEEALSEFSFGSLVWLSGKYVVKSPI 776
             ++I V P KL+F +    L Y V+F   V  G   ++    FGS+ W  G++VVKSP+
Sbjct: 735 ANVEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPV 769

BLAST of Lag0041267 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 3.4e-197
Identity = 383/784 (48.85%), Postives = 499/784 (63.65%), Query Frame = 0

Query: 15  AASTAALDQQTYIIHMDTTKMPTTNPQ--QWYTSMIDSVNELASLDEDEEEVSNAAEIFY 74
           ++S ++ +  TYI+H+D    P+  P    WYTS       LASL       S+   I +
Sbjct: 17  SSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTS------SLASL------TSSPPSIIH 76

Query: 75  VYKNAISGKELKCVLDKYKNNGFAAKLSTKKLNSLSQTPGFLTAIPDQLLQLHTTHSPHF 134
            Y                  +GF+A+L+++  + L   P  ++ IP+Q+  LHTT SP F
Sbjct: 77  TYDTVF--------------HGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEF 136

Query: 135 LGLKTGH--GLWNATNLASDVIVGVVDTGIWPEHVSFEDKGLPPVPRRWKGGCEEGQKFS 194
           LGL++    GL   ++  SD+++GV+DTG+WPE  SF+D+GL PVP +WKG C   Q F 
Sbjct: 137 LGLRSTDKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFP 196

Query: 195 ASNCNRKLIGARAFIKAYEASFGRLNESGVFRSPRDWNGHGTHTASTAAGNLVFKAGFYN 254
            S CNRKL+GAR F   YEA+ G++NE+  FRSPRD +GHGTHTAS +AG  VF A    
Sbjct: 197 ESACNRKLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLG 256

Query: 255 HALGNAAGIRFTSRIAAYKVCWPLGCAIADVLAAMDRAVADGVDVLSLSLGGNPVPFYND 314
           +A G AAG+   +R+AAYKVCW  GC  +D+LAA D AVADGVDV+SLS+GG  VP+Y D
Sbjct: 257 YAHGVAAGMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLD 316

Query: 315 GLAIAAFGATQKGVFVSCAAGNSGPAPSTAGNLAPWIMTVGASYTDRIFPTTVKLGNGQV 374
            +AI AFGA  +G+FVS +AGN GP   T  N+APW+ TVGA   DR FP  VKLGNG++
Sbjct: 317 AIAIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKM 376

Query: 375 FEGSSLFSGNNMG---QLPLVYKQGRQG-----ANLCLAGSLVPSMVRGKIVICERGTNT 434
             G S++ G  +      PLVY     G     ++LCL GSL P++V+GKIV+C+RG N+
Sbjct: 377 ISGVSVYGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINS 436

Query: 435 RIGKGEQVKLAGGAGMILINTQLEGEELFADPHVLPATSLGAAAGKAIKNYIA------S 494
           R  KGE V+  GG GMI+ N   +GE L AD HVLPATS+GA+ G  I+ YI+      S
Sbjct: 437 RATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRS 496

Query: 495 SKQPKASIAFQGTKYGSR-APRVAAFSSRGPSSIEPNIIKPDIIAPGVNILAAWPSIVSP 554
           SK P A+I F+GT+ G R AP VA+FS+RGP+   P I+KPD+IAPG+NILAAWP  + P
Sbjct: 497 SKHPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGP 556

Query: 555 SELEFDKRRVLFNIVSGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTNDNKMS 614
           S +  D RR  FNI+SGTSM+CPHVSGLAALLKAAH DWSPAAI+SAL+TTAYT DN   
Sbjct: 557 SGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGE 616

Query: 615 PISDVGSANGKPANPFAFGSGHVDPEKASDPGLVYDITPEDYLNYLCSLNYNSTQIALVS 674
           P+ D   + G  ++   +GSGHV P KA DPGLVYDIT  DY+N+LC+ NY  T I  ++
Sbjct: 617 PMMD--ESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTIT 676

Query: 675 RGSFTCPSKRTFLQPGDLNYPSFS-VFMKKKAKNVTVTLKRTVTNVGSPMSDYTVQINNP 734
           R    C   R     G+LNYPSFS VF +     ++    RTVTNVG   S Y ++I  P
Sbjct: 677 RRQADCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPP 736

Query: 735 KGIKISVKPEKLSFGRLGEKLSY--RVSFVAFGKEEALSEFSFGSLVWLSGKYVVKSPIA 777
           +G  ++V+PEKLSF R+G+KLS+  RV           +    G +VW  GK  V SP+ 
Sbjct: 737 RGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLV 772

BLAST of Lag0041267 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 676.0 bits (1743), Expect = 5.0e-193
Identity = 367/762 (48.16%), Postives = 484/762 (63.52%), Query Frame = 0

Query: 23  QQTYIIHMDTTKMPTT--NPQQWYTSMIDSVNELASLDEDEEEVSNAAEIFYVYKNAISG 82
           ++TYII ++ +  P +      WYTS ++S + L                 Y Y  +   
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSESSL----------------LYTYTTSF-- 86

Query: 83  KELKCVLDKYKNNGFAAKLSTKKLNS-LSQTPGFLTAIPDQLLQLHTTHSPHFLGLKTGH 142
                       +GF+A L + + +S LS +   L    D L  LHTT +P FLGL +  
Sbjct: 87  ------------HGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEF 146

Query: 143 GLWNATNLASDVIVGVVDTGIWPEHVSFEDKGLPPVPRRWKGGCEEGQKFSASNCNRKLI 202
           G+ +  + ++ VI+GV+DTG+WPE  SF+D  +P +P +WKG CE G  F +  CN+KLI
Sbjct: 147 GVHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLI 206

Query: 203 GARAFIKAYE-ASFGRLNESGVFRSPRDWNGHGTHTASTAAGNLVFKAGFYNHALGNAAG 262
           GAR+F K ++ AS G  +      SPRD +GHGTHT++TAAG+ V  A F  +A G A G
Sbjct: 207 GARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARG 266

Query: 263 IRFTSRIAAYKVCWPLGCAIADVLAAMDRAVADGVDVLSLSLGGNPVPFYNDGLAIAAFG 322
           +   +R+A YKVCW  GC  +D+LAAMDRA+ DGVDVLSLSLGG   P+Y D +AI AF 
Sbjct: 267 MATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFS 326

Query: 323 ATQKGVFVSCAAGNSGPAPSTAGNLAPWIMTVGASYTDRIFPTTVKLGNGQVFEGSSLFS 382
           A ++GVFVSC+AGNSGP  ++  N+APW+MTVGA   DR FP    LGNG+   G SL+S
Sbjct: 327 AMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYS 386

Query: 383 GNNMGQLP--LVYKQGR-QGANLCLAGSLVPSMVRGKIVICERGTNTRIGKGEQVKLAGG 442
           G  MG  P  LVY +G    +NLCL GSL  S+VRGKIV+C+RG N R+ KG  V+ AGG
Sbjct: 387 GVGMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGG 446

Query: 443 AGMILINTQLEGEELFADPHVLPATSLGAAAGKAIKNYIASSKQPKASIAFQGTKYGSR- 502
            GMI+ NT   GEEL AD H+LPA ++G   G  ++ Y+ S  +P A + F+GT    + 
Sbjct: 447 LGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKP 506

Query: 503 APRVAAFSSRGPSSIEPNIIKPDIIAPGVNILAAWPSIVSPSELEFDKRRVLFNIVSGTS 562
           +P VAAFSSRGP+++ P I+KPD+I PGVNILA W   + P+ L+ D RR  FNI+SGTS
Sbjct: 507 SPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTS 566

Query: 563 MSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTNDNKMSPISDVGSANGKPANPFAFG 622
           MSCPH+SGLA LLKAAH +WSP+AIKSALMTTAY  DN  +P+ D  +A+   +NP+A G
Sbjct: 567 MSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHD--AADNSLSNPYAHG 626

Query: 623 SGHVDPEKASDPGLVYDITPEDYLNYLCSLNYNSTQI-ALVSRGSFTCPSKRTFLQPGDL 682
           SGHVDP+KA  PGLVYDI+ E+Y+ +LCSL+Y    I A+V R S  C  K  F  PG L
Sbjct: 627 SGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKK--FSDPGQL 686

Query: 683 NYPSFSVFMKKKAKNVTVTLKRTVTNVGSPMSDYTVQINNPKGIKISVKPEKLSFGRLGE 742
           NYPSFSV    K     V   R VTNVG+  S Y V +N    + ISVKP KLSF  +GE
Sbjct: 687 NYPSFSVLFGGKR---VVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGE 746

Query: 743 KLSYRVSFVAFGKEEALSEFSFGSLVWLSGKYVVKSPIAVTW 776
           K  Y V+FV+       ++  FGS+ W + ++ V+SP+A +W
Sbjct: 747 KKRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSW 751

BLAST of Lag0041267 vs. ExPASy TrEMBL
Match: A0A6J1FMR4 (subtilisin-like protease SBT1.1 OS=Cucurbita moschata OX=3662 GN=LOC111446877 PE=3 SV=1)

HSP 1 Score: 1192.2 bits (3083), Expect = 0.0e+00
Identity = 597/779 (76.64%), Postives = 675/779 (86.65%), Query Frame = 0

Query: 1   MAFREVWVLLCIVFAASTAALDQQTYIIHMDTTKMPTTNPQQWYTSMIDSVNELASLDED 60
           M FREV + L I  A STAA+DQQ+YIIHMDTTKM    P+QWYT++IDS+NE++SL ED
Sbjct: 1   MGFREVCLFLSIFLATSTAAVDQQSYIIHMDTTKMAAPTPEQWYTALIDSINEISSL-ED 60

Query: 61  EEEVSNAAEIFYVYKNAISGKELKCVLDKYKNNGFAAKLSTKKLNSLSQTPGFLTAIPDQ 120
           +EE SNAA+I YVYK AIS              GFAAKLSTKKL+SLS+TPGFL A P++
Sbjct: 61  QEEASNAAQILYVYKTAIS--------------GFAAKLSTKKLHSLSKTPGFLAATPNE 120

Query: 121 LLQLHTTHSPHFLGLKTGHGLWNATNLASDVIVGVVDTGIWPEHVSFEDKGLPPVPRRWK 180
           LLQLHTTHSP FLGL+  HGLWN++NLASD+++G++DTGIWPEH+SF+DKGLPPVP++WK
Sbjct: 121 LLQLHTTHSPQFLGLQREHGLWNSSNLASDIVIGLLDTGIWPEHISFQDKGLPPVPKKWK 180

Query: 181 GGCEEGQKFSASNCNRKLIGARAFIKAYEASFGRLNESGVFRSPRDWNGHGTHTASTAAG 240
           G C+ GQKFS SNCNRKLIGA A+IK YEA  GRLN +G FRSPRD +GHGTHTASTAAG
Sbjct: 181 GTCQAGQKFSPSNCNRKLIGAIAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAG 240

Query: 241 NLVFKAGFYNHALGNAAGIRFTSRIAAYKVCWPLGCAIADVLAAMDRAVADGVDVLSLSL 300
           N+V KA F+N A+G A G+RFTSRIAAYKVCW  GCA AD+LAA+DRAVADGVDVLSLSL
Sbjct: 241 NIVNKASFFNQAMGVATGMRFTSRIAAYKVCWTEGCANADILAAIDRAVADGVDVLSLSL 300

Query: 301 GGNPVPFYNDGLAIAAFGATQKGVFVSCAAGNSGPAPSTAGNLAPWIMTVGASYTDRIFP 360
           GG+   FY D +AIA FGA + GVFVSC+AGNSGP+ ST  N+APWIMTV ASYTDR FP
Sbjct: 301 GGSASAFYKDDIAIATFGAVRNGVFVSCSAGNSGPSSSTVSNIAPWIMTVAASYTDRSFP 360

Query: 361 TTVKLGNGQVFEGSSLFSGNNMGQLPLVYKQ--GRQGANLCLAGSLVPSMVRGKIVICER 420
            TVKLGNGQVFEGSSL+SGNN+GQLPLVY    G + AN+C AGSLVPSMV+GKIV+CER
Sbjct: 361 ATVKLGNGQVFEGSSLYSGNNIGQLPLVYNNTAGGEDANVCTAGSLVPSMVKGKIVVCER 420

Query: 421 GTNTRIGKGEQVKLAGGAGMILINTQLEGEELFADPHVLPATSLGAAAGKAIKNYIASSK 480
           GTN+R  KGEQVKLAGGAGMILINTQLEGEELFADPHVLPA +LGA+AG+AI  YI+SSK
Sbjct: 421 GTNSRTAKGEQVKLAGGAGMILINTQLEGEELFADPHVLPAANLGASAGQAIIRYISSSK 480

Query: 481 -QPKASIAFQGTKYGSRAPRVAAFSSRGPSSIEPNIIKPDIIAPGVNILAAWPSIVSPSE 540
            QPKA IAF+GTK+G+RAPRVAAFSSRGPS I P++IKPD+ APGVNILAAWP I SPSE
Sbjct: 481 HQPKALIAFEGTKFGNRAPRVAAFSSRGPSLIAPDVIKPDVTAPGVNILAAWPLIASPSE 540

Query: 541 LEFDKRRVLFNIVSGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTNDNKMSPI 600
           +E DKRRVLFN++SGTSMSCPHVSGLAALLK+AHKDWSPAAIKSALMTTAYTNDN+MSPI
Sbjct: 541 VESDKRRVLFNVISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNRMSPI 600

Query: 601 SDVGSANGKPANPFAFGSGHVDPEKASDPGLVYDITPEDYLNYLCSLNYNSTQIALVSRG 660
           SDVGSA+GKPANPFAFGSGHVDPEKASDPGL+YDITP+DYLNYLCSLNYNSTQI LVSRG
Sbjct: 601 SDVGSASGKPANPFAFGSGHVDPEKASDPGLIYDITPQDYLNYLCSLNYNSTQIGLVSRG 660

Query: 661 SFTCPSKRTFLQPGDLNYPSFSVFMKKKAKNVTVTLKRTVTNVGSPMSDYTVQINNPKGI 720
           +FTCPSKR   Q G LNYPSFSVFMKKKAKNV+VTLKRTVTNVG P SDY+V+I NPKGI
Sbjct: 661 NFTCPSKRRVGQAGKLNYPSFSVFMKKKAKNVSVTLKRTVTNVGRPRSDYSVKIKNPKGI 720

Query: 721 KISVKPEKLSFGRLGEKLSYRVSFVAFGKEEALSEFSFGSLVWLSGKYVVKSPIAVTWQ 777
            ISVKPEKLSF R G+KLSY+VSFVA GK E +S FSFGSLVW+SG Y V+SPIAVTW+
Sbjct: 721 GISVKPEKLSFRRYGQKLSYQVSFVALGKREGVSGFSFGSLVWVSGTYAVRSPIAVTWK 764

BLAST of Lag0041267 vs. ExPASy TrEMBL
Match: A0A6J1JPC3 (subtilisin-like protease SBT1.1 OS=Cucurbita maxima OX=3661 GN=LOC111487676 PE=3 SV=1)

HSP 1 Score: 1186.4 bits (3068), Expect = 0.0e+00
Identity = 593/779 (76.12%), Postives = 674/779 (86.52%), Query Frame = 0

Query: 1   MAFREVWVLLCIVFAASTAALDQQTYIIHMDTTKMPTTNPQQWYTSMIDSVNELASLDED 60
           M FREV + L I  A S AA+DQQ+YIIHMDTTKM   +P+QWYT++IDS+N+++SLD D
Sbjct: 1   MGFREVCLFLSIFLATSAAAVDQQSYIIHMDTTKMAAPSPEQWYTALIDSINKISSLD-D 60

Query: 61  EEEVSNAAEIFYVYKNAISGKELKCVLDKYKNNGFAAKLSTKKLNSLSQTPGFLTAIPDQ 120
           +EE S+AA+I YVYK AIS              GFAAKLSTKKL+SLS+TPGFL A P++
Sbjct: 61  QEEASSAAQILYVYKTAIS--------------GFAAKLSTKKLHSLSKTPGFLAATPNE 120

Query: 121 LLQLHTTHSPHFLGLKTGHGLWNATNLASDVIVGVVDTGIWPEHVSFEDKGLPPVPRRWK 180
           LLQLHTTHSP FLGL+  HGLWN++NLASD+++G++DTGIWPEH+SF+DKGLPPVP++WK
Sbjct: 121 LLQLHTTHSPQFLGLQREHGLWNSSNLASDIVIGLLDTGIWPEHISFQDKGLPPVPKKWK 180

Query: 181 GGCEEGQKFSASNCNRKLIGARAFIKAYEASFGRLNESGVFRSPRDWNGHGTHTASTAAG 240
           G C+ GQKFS SNCNRKLIGA A+IK YEA  GRLN +G FRSPRD +GHGTHTASTAAG
Sbjct: 181 GTCQAGQKFSPSNCNRKLIGATAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAG 240

Query: 241 NLVFKAGFYNHALGNAAGIRFTSRIAAYKVCWPLGCAIADVLAAMDRAVADGVDVLSLSL 300
           N+V KA F+N A+G A G+RFTSRIAAYKVCW  GCA AD+LAA+DRAVADGVDVLSLSL
Sbjct: 241 NIVNKASFFNQAMGVATGMRFTSRIAAYKVCWTEGCANADILAAIDRAVADGVDVLSLSL 300

Query: 301 GGNPVPFYNDGLAIAAFGATQKGVFVSCAAGNSGPAPSTAGNLAPWIMTVGASYTDRIFP 360
           GG+   FY D +AIA FGA + GVFVSC+AGNSGP+ ST  N+APWIMTV ASYTDR FP
Sbjct: 301 GGSASAFYKDDIAIATFGAVRNGVFVSCSAGNSGPSSSTVSNIAPWIMTVAASYTDRTFP 360

Query: 361 TTVKLGNGQVFEGSSLFSGNNMGQLPLVYKQ--GRQGANLCLAGSLVPSMVRGKIVICER 420
            TVKLGNGQVFEGSSL+SGN++GQLPLVY    G + AN+C AGSLVPS+V+GKIV+CER
Sbjct: 361 ATVKLGNGQVFEGSSLYSGNSIGQLPLVYNNTAGGEEANVCTAGSLVPSLVKGKIVVCER 420

Query: 421 GTNTRIGKGEQVKLAGGAGMILINTQLEGEELFADPHVLPATSLGAAAGKAIKNYIASSK 480
           GTN+R  KGEQVKLAGGAGMILINTQLEGEELFADPHVLPA +LGA+AG+AI  YI+SSK
Sbjct: 421 GTNSRTAKGEQVKLAGGAGMILINTQLEGEELFADPHVLPAANLGASAGQAIIRYISSSK 480

Query: 481 -QPKASIAFQGTKYGSRAPRVAAFSSRGPSSIEPNIIKPDIIAPGVNILAAWPSIVSPSE 540
            QPKA IAF+GTK+G+RAPRVAAFSSRGPS I P++IKPD+ APGVNILAAWP I SPSE
Sbjct: 481 HQPKALIAFEGTKFGNRAPRVAAFSSRGPSLIAPDVIKPDVTAPGVNILAAWPLIASPSE 540

Query: 541 LEFDKRRVLFNIVSGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTNDNKMSPI 600
           LE DKRRVLFNI+SGTSMSCPHVSGLAALLK+AHKDWSPAAIKSALMTTAYTNDN+MSPI
Sbjct: 541 LESDKRRVLFNIISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNRMSPI 600

Query: 601 SDVGSANGKPANPFAFGSGHVDPEKASDPGLVYDITPEDYLNYLCSLNYNSTQIALVSRG 660
           SDVGS +GKPANPFAFGSGHVDPEKASDPGL+YDITP+DYLNY CSLNYNSTQI LVSRG
Sbjct: 601 SDVGSESGKPANPFAFGSGHVDPEKASDPGLIYDITPQDYLNYFCSLNYNSTQIGLVSRG 660

Query: 661 SFTCPSKRTFLQPGDLNYPSFSVFMKKKAKNVTVTLKRTVTNVGSPMSDYTVQINNPKGI 720
           +FTCPSKR   QPG+LNYPSFSVFMKKKAKNV+VTLKRTVTNVG P SDYTV+I NPKGI
Sbjct: 661 NFTCPSKRRVGQPGNLNYPSFSVFMKKKAKNVSVTLKRTVTNVGRPRSDYTVKIKNPKGI 720

Query: 721 KISVKPEKLSFGRLGEKLSYRVSFVAFGKEEALSEFSFGSLVWLSGKYVVKSPIAVTWQ 777
            I V+PEKLSF R G+KLSY+VSFVA GK E L  FSFGSLVW+SGKY V+SPIAVTW+
Sbjct: 721 GIRVEPEKLSFRRYGQKLSYQVSFVALGKREGLGGFSFGSLVWVSGKYGVRSPIAVTWK 764

BLAST of Lag0041267 vs. ExPASy TrEMBL
Match: A0A6J1D8S5 (subtilisin-like protease SBT1.1 OS=Momordica charantia OX=3673 GN=LOC111018293 PE=3 SV=1)

HSP 1 Score: 1162.9 bits (3007), Expect = 0.0e+00
Identity = 583/782 (74.55%), Postives = 670/782 (85.68%), Query Frame = 0

Query: 1   MAFREVWVLLCIVFAAST--AALDQQTYIIHMDTTKMPTTNPQQWYTSMIDSVNELASLD 60
           M  REVWV+L I+ A S+  AA+DQ+T+IIHMDTTKMP T+P+QWYTSMI SVNEL SL+
Sbjct: 1   MGLREVWVVLSIMVATSSAAAAVDQRTFIIHMDTTKMPATDPEQWYTSMIHSVNELPSLN 60

Query: 61  EDEEEVSNAAEIFYVYKNAISGKELKCVLDKYKNNGFAAKLSTKKLNSLSQTPGFLTAIP 120
            DE E SN AE+ YVYK A+S               FAAKL+T+KL+++S+  GF+ AIP
Sbjct: 61  TDEGEASNTAEVLYVYKTALS--------------XFAAKLTTRKLDAISKIRGFVAAIP 120

Query: 121 DQLLQLHTTHSPHFLGLKTGHGLWNATNLASDVIVGVVDTGIWPEHVSFEDKGLPPVPRR 180
           D+LLQLHTTHS +FLGL+  HGLWN++ LASD+IVGV+DTGIWPEHVSF DKG+P VPRR
Sbjct: 121 DELLQLHTTHSSNFLGLELDHGLWNSSTLASDIIVGVLDTGIWPEHVSFHDKGVPRVPRR 180

Query: 181 WKGGCEEGQKFSASNCNRKLIGARAFIKAYEASFGRLNESGVFRSPRDWNGHGTHTASTA 240
           WKG CE+G KFS SNCNRKLIGA AF+K YE+  GRLN++  +RSPRD +GHGTHTASTA
Sbjct: 181 WKGVCEQGPKFSPSNCNRKLIGAAAFLKGYESIVGRLNQTATYRSPRDSDGHGTHTASTA 240

Query: 241 AGNLVFKAGFYNHALGNAAGIRFTSRIAAYKVCWPLGCAIADVLAAMDRAVADGVDVLSL 300
           AGNLV+KAGFY+ A+G AAG+RFTSRIAAYKVCWP GCA AD+LAA+DRAVADGVDVLSL
Sbjct: 241 AGNLVYKAGFYDQAMGAAAGMRFTSRIAAYKVCWPAGCANADILAAIDRAVADGVDVLSL 300

Query: 301 SLGGNPVPFYNDGLAIAAFGATQKGVFVSCAAGNSGPAPSTAGNLAPWIMTVGASYTDRI 360
           SLGG   PFY DG++IAAFGA + GVFVSC+AGNSGP+PST  N+APWIMTV ASYTDR 
Sbjct: 301 SLGGRAAPFYRDGISIAAFGAVRNGVFVSCSAGNSGPSPSTVSNVAPWIMTVAASYTDRT 360

Query: 361 FPTTVKLGNGQVFEGSSLFSGNNMGQLPLVYKQ--GRQGANLCLAGSLVPSMVRGKIVIC 420
           FPTTVKLG+GQVF+GSSL+SG N  QLPLVY +  G Q ANLC  GSL+P+MV+GKIV+C
Sbjct: 361 FPTTVKLGSGQVFQGSSLYSGKNTNQLPLVYNKTAGPQEANLCTPGSLIPAMVKGKIVVC 420

Query: 421 ERGTNTRIGKGEQVKLAGGAGMILINTQLEGEELFADPHVLPATSLGAAAGKAIKNYIAS 480
           ERGT +R  KGEQVKLAGGA MILINT+LEGEELFADPHVLPATSLGA+AGKAIK +IAS
Sbjct: 421 ERGTISRTAKGEQVKLAGGAAMILINTELEGEELFADPHVLPATSLGASAGKAIKKFIAS 480

Query: 481 SK-QPKASIAFQGTKYGSRAPRVAAFSSRGPSSIEPNIIKPDIIAPGVNILAAWPSIVSP 540
           SK QPK SI F GT+YGSRAPRVAAFSSRGP+ I+  I+KPD+ APGVNILAAWP+I+SP
Sbjct: 481 SKDQPKGSITFGGTRYGSRAPRVAAFSSRGPNPIQRTIMKPDVTAPGVNILAAWPAIISP 540

Query: 541 SELEFDKRRVLFNIVSGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTNDNKMS 600
           SELE DKRRVLFNI+SGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYT DNKM 
Sbjct: 541 SELESDKRRVLFNIISGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTTDNKMR 600

Query: 601 PISDVGSANGKPANPFAFGSGHVDPEKASDPGLVYDITPEDYLNYLCSLNYNSTQIALVS 660
            ISDVGS +G+PAN FAFGSGHVDPEKASDPGLVYDITP DYLNYLCSLNY S+QIAL+S
Sbjct: 601 LISDVGSTSGRPANAFAFGSGHVDPEKASDPGLVYDITPRDYLNYLCSLNYTSSQIALLS 660

Query: 661 RGSFTCPSKRTFLQPGDLNYPSFSVFM-KKKAKNVTVTLKRTVTNVGSPMSDYTVQINNP 720
           R +F+CPS+  FL+PGDLNYPSFSV M +KKA NV+VTLKRTVTNVGSP  DYTV+IN+P
Sbjct: 661 RENFSCPSRGAFLKPGDLNYPSFSVIMDQKKATNVSVTLKRTVTNVGSPRGDYTVKINSP 720

Query: 721 KGIKISVKPEKLSFGRLGEKLSYRVSFVAFGKEEALSEFSFGSLVWLSGKYVVKSPIAVT 777
           KG+ I VKP+KLSF RLGEKLSY+VSF++ GK ++  + SFGSLVWLSGKY V+SP+AVT
Sbjct: 721 KGVAIIVKPQKLSFKRLGEKLSYKVSFISLGKGKSFGDSSFGSLVWLSGKYSVRSPVAVT 768

BLAST of Lag0041267 vs. ExPASy TrEMBL
Match: A0A1S3C0J8 (subtilisin-like protease SBT1.1 OS=Cucumis melo OX=3656 GN=LOC103495084 PE=3 SV=1)

HSP 1 Score: 1156.4 bits (2990), Expect = 0.0e+00
Identity = 582/780 (74.62%), Postives = 661/780 (84.74%), Query Frame = 0

Query: 1   MAFREVWVLLCIVFAASTAALDQQTYIIHMDTTKMPTTNPQQWYTSMIDSVNELASLDED 60
           M FREVWV L I+ A S+A +DQQTYIIHMDTTKM T NP+QWYT +IDSVNEL+SLD D
Sbjct: 1   MGFREVWV-LSIMLAISSAVVDQQTYIIHMDTTKMVTPNPEQWYTDIIDSVNELSSLD-D 60

Query: 61  EEEVSNAAEIFYVYKNAISGKELKCVLDKYKNNGFAAKLSTKKLNSLSQTPGFLTAIPDQ 120
            EE SNAAEI YVYK A+S              GFAAKL++KKL+SLS+ PGFL A P++
Sbjct: 61  NEEASNAAEILYVYKTALS--------------GFAAKLTSKKLHSLSKIPGFLAATPNE 120

Query: 121 LLQLHTTHSPHFLGLKTGHGLWNATNLASDVIVGVVDTGIWPEHVSFEDKGLPPVPRRWK 180
           LLQLHTTHSP FLGL+  HGLWN +NLASD+I+G++DTGIWPEH+SF+DKGL  VP +WK
Sbjct: 121 LLQLHTTHSPQFLGLQRDHGLWNFSNLASDIIIGLLDTGIWPEHISFQDKGLSSVPLKWK 180

Query: 181 GGCEEGQKFSASNCNRKLIGARAFIKAYEASFGRLNESGVFRSPRDWNGHGTHTASTAAG 240
           G C+ G +FS+SNCN+KLIGA A+IK YEA  GRLNE+G FRSPRD +GHGTHTASTAAG
Sbjct: 181 GICQTGPRFSSSNCNKKLIGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAG 240

Query: 241 NLVFKAGFYNHALGNAAGIRFTSRIAAYKVCWPLGCAIADVLAAMDRAVADGVDVLSLSL 300
           ++V  A FYN  +G A+G+RFTSRI AYKVCWPLGCA AD+LAAMD AVADGVDVLSLSL
Sbjct: 241 SIVDNASFYNQGMGVASGMRFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSL 300

Query: 301 GGNPVPFYNDGLAIAAFGATQKGVFVSCAAGNSGPAPSTAGNLAPWIMTVGASYTDRIFP 360
           GG    FY D +AIAAFGA QKGVFVSC+AGNSGP+PST GN APWIMTV ASYTDR FP
Sbjct: 301 GGGSSSFYKDNIAIAAFGAIQKGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFP 360

Query: 361 TTVKLGNGQVFEGSSLFSGNNMGQLPLVYKQ---GRQGANLCLAGSLVPSMVRGKIVICE 420
           TTVKLGNGQVFEGSSL+ G ++ +LPLVY       Q  N+C+AGSL PSMV+GKIVICE
Sbjct: 361 TTVKLGNGQVFEGSSLYYGKSINELPLVYNNTAGDGQETNVCIAGSLDPSMVKGKIVICE 420

Query: 421 RGTNTRIGKGEQVKLAGGAGMILINTQLEGEELFADPHVLPATSLGAAAGKAIKNYIASS 480
           RGT +R  KGEQVKLAGG GMILINTQ EGEELFADPHVLPAT+LGA+AGKAI +YIASS
Sbjct: 421 RGTISRTEKGEQVKLAGGTGMILINTQFEGEELFADPHVLPATTLGASAGKAILDYIASS 480

Query: 481 K-QPKASIAFQGTKYGSRAPRVAAFSSRGPSSIEPNIIKPDIIAPGVNILAAWPSIVSPS 540
           K Q KASI F+GTKYGS+APRVAAFSSRGPS + P++IKPD+ APGVNILAAWP IVSPS
Sbjct: 481 KTQAKASIVFEGTKYGSQAPRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPS 540

Query: 541 ELEFDKRRVLFNIVSGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTNDNKMSP 600
           EL  D RRV+FNI+SGTSMSCPHVSGLAALLK+AH DWSPAAIKSALMTTAY  D+KMS 
Sbjct: 541 ELASDTRRVMFNIISGTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYVTDSKMSL 600

Query: 601 ISDVGSANGKPANPFAFGSGHVDPEKASDPGLVYDITPEDYLNYLCSLNYNSTQIALVSR 660
           ISDVG ANG+PA PF FGSGHVDPEKASDPGL+YDITP+DY+NYLCSL YNS+QIALVSR
Sbjct: 601 ISDVGQANGEPATPFTFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNSSQIALVSR 660

Query: 661 GSFTCPSKRTFLQPGDLNYPSFSVFMKKKAKNVTVTLKRTVTNVGSPMSDYTVQINNPKG 720
           G+ TC SKRT ++PGDLNYPSFSVFMKKKAK V++TLKRTVTNVG   SDYTV+INNPKG
Sbjct: 661 GNLTCSSKRTVVKPGDLNYPSFSVFMKKKAKKVSITLKRTVTNVGITRSDYTVKINNPKG 720

Query: 721 IKISVKPEKLSFGRLGEKLSYRVSFVAFGKEEALSEFSFGSLVWLSGKYVVKSPIAVTWQ 777
           + + VKPEKLSFG LGE+LSY+VSFV+ G +EAL +FSFGSLVW+SGKY V+SPI VTWQ
Sbjct: 721 VTVIVKPEKLSFGSLGEQLSYKVSFVSLGGKEALDKFSFGSLVWISGKYAVRSPIVVTWQ 764

BLAST of Lag0041267 vs. ExPASy TrEMBL
Match: A0A6J1JN56 (subtilisin-like protease SBT1.1 OS=Cucurbita maxima OX=3661 GN=LOC111486706 PE=3 SV=1)

HSP 1 Score: 1152.5 bits (2980), Expect = 0.0e+00
Identity = 574/780 (73.59%), Postives = 660/780 (84.62%), Query Frame = 0

Query: 1   MAFREVW-VLLCIVFAASTAALDQQTYIIHMDTTKMPTTNPQQWYTSMIDSVNELASLDE 60
           M   E+W +LL I+ A S AA+DQQTYIIHMD TKM TTNP+QWYTS+I S+N+L+S+++
Sbjct: 1   MGITELWLLLLSIMLATSAAAVDQQTYIIHMDATKMATTNPEQWYTSIIHSINQLSSIND 60

Query: 61  DEEEVSNAAEIFYVYKNAISGKELKCVLDKYKNNGFAAKLSTKKLNSLSQTPGFLTAIPD 120
           D+ E SNAAEI Y+YK AIS              GF+AKLST+KL+SLS+ PGFL A PD
Sbjct: 61  DQNEASNAAEILYIYKTAIS--------------GFSAKLSTRKLHSLSKLPGFLAATPD 120

Query: 121 QLLQLHTTHSPHFLGLKTGHGLWNATNLASDVIVGVVDTGIWPEHVSFEDKGLPPVPRRW 180
           +LLQLHTTH+P FLGL+ GHGLWNA+NLASD+I+GV+DTGIWPEH+SF+DKGLPPVP++W
Sbjct: 121 KLLQLHTTHTPQFLGLQRGHGLWNASNLASDIIIGVIDTGIWPEHISFQDKGLPPVPKKW 180

Query: 181 KGGCEEGQKFSASNCNRKLIGARAFIKAYEASFGRLNESGVFRSPRDWNGHGTHTASTAA 240
           KG C+ G KFS SNCN+KL+GARA+IK YE   GRLN +G FRS RD +GHGTHTASTAA
Sbjct: 181 KGTCQAGPKFSRSNCNKKLVGARAYIKGYETILGRLNTTGTFRSARDSDGHGTHTASTAA 240

Query: 241 GNLVFKAGFYNHALGNAAGIRFTSRIAAYKVCWPLGCAIADVLAAMDRAVADGVDVLSLS 300
           GN+V+KA  YN  +G A G+RFTSRIAAYKVCWP GCA  D+LAA+DRAV DGVDVLSLS
Sbjct: 241 GNIVYKASLYNQGMGAATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVVDGVDVLSLS 300

Query: 301 LGGNPVPFYNDGLAIAAFGATQKGVFVSCAAGNSGPAPSTAGNLAPWIMTVGASYTDRIF 360
           LGG    FY D +AIAAFGA + GVFVSC+AGNSGP  ST GN+APWIMTV ASYTDR F
Sbjct: 301 LGGGDGFFYQDEIAIAAFGAVRNGVFVSCSAGNSGPFMSTVGNVAPWIMTVAASYTDRTF 360

Query: 361 PTTVKLGNGQVFEGSSLFSGNNMGQLPLVYKQ--GRQGANLCLAGSLVPSMVRGKIVICE 420
           P +VKLGNGQ+FEGSSL SGN++G+LPLVY +  G + A++C AGSLVPSMV+GKIV+CE
Sbjct: 361 PGSVKLGNGQIFEGSSLHSGNSIGELPLVYNKTAGGEEASVCTAGSLVPSMVKGKIVVCE 420

Query: 421 RGTNTRIGKGEQVKLAGGAGMILINTQLEGEELFADPHVLPATSLGAAAGKAIKNYIASS 480
           RGTN+R  KGEQVKLAGG GMILINTQLEGEELF D HVLPA +LGA+AGKAI NYIASS
Sbjct: 421 RGTNSRFEKGEQVKLAGGVGMILINTQLEGEELFGDSHVLPAINLGASAGKAIINYIASS 480

Query: 481 KQ-PKASIAFQGTKYGSRAPRVAAFSSRGPSSIEPNIIKPDIIAPGVNILAAWPSIVSPS 540
           K  PKASI F+GT+YGSRAPR+AAFSSRGPS  EP +IKPDI APGVNILAAWP +VSPS
Sbjct: 481 KHPPKASILFEGTRYGSRAPRMAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPPVVSPS 540

Query: 541 ELEFDKRRVLFNIVSGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTNDNKMSP 600
           EL+ DKRRVLFNI+SGTSMSCPHVSG+AALLK+AHK+WSPAAIKSALMTTAY NDNK S 
Sbjct: 541 ELKSDKRRVLFNIISGTSMSCPHVSGIAALLKSAHKNWSPAAIKSALMTTAYVNDNKRSL 600

Query: 601 ISDVGSANGKPANPFAFGSGHVDPEKASDPGLVYDITPEDYLNYLCSLNYNSTQIALVSR 660
           ISDVG  +G PA+P+AFGSGHVDPEKA DPGLVYDI P+DYLNYLCSLNY S Q+ LVSR
Sbjct: 601 ISDVGRPSGGPADPYAFGSGHVDPEKAVDPGLVYDIVPQDYLNYLCSLNYTSKQVGLVSR 660

Query: 661 GSFTCPSKRTFLQPGDLNYPSFSVFMKKKAKNVTVTLKRTVTNVGSPMSDYTVQINNPKG 720
           G+F+CPSKRT LQPGDLNYPSFSV MK KAKN  V  KRTVTNVG+P SDYTV+INNP G
Sbjct: 661 GNFSCPSKRTVLQPGDLNYPSFSVSMKNKAKN--VRFKRTVTNVGTPSSDYTVKINNPSG 720

Query: 721 IKISVKPEKLSFGRLGEKLSYRVSFVAFGKEEALSEFSFGSLVWLSGKYVVKSPIAVTWQ 777
           I++SVKPEKLSF R G+KLSY+VSFVA GK E LS+FSFGSLVW+SGKY V+SPIAV W+
Sbjct: 721 IRVSVKPEKLSFRRSGQKLSYQVSFVALGKREGLSDFSFGSLVWVSGKYSVRSPIAVNWE 764

BLAST of Lag0041267 vs. TAIR 10
Match: AT1G01900.1 (subtilase family protein )

HSP 1 Score: 808.5 bits (2087), Expect = 4.6e-234
Identity = 423/780 (54.23%), Postives = 541/780 (69.36%), Query Frame = 0

Query: 1   MAFREVWVLLCIVFAASTAALDQQTYIIHMDTTKMPTTNPQQWYTSMIDSVNELASLDED 60
           M FR   V   ++F AS  +  +QTY+IH       TT+ +   TS+ +S+ +  ++++D
Sbjct: 19  MFFRSFIVFFFLIFFASNVSSRKQTYVIH-----TVTTSTKHIVTSLFNSL-QTENINDD 78

Query: 61  EEEVSNAAEIFYVYKNAISGKELKCVLDKYKNNGFAAKLSTKKLNSLSQTPGFLTAIPDQ 120
           +    +  EI Y+Y+NA+S              GF+A L+  +L+++  T GF++A PD+
Sbjct: 79  D---FSLPEIHYIYENAMS--------------GFSATLTDDQLDTVKNTKGFISAYPDE 138

Query: 121 LLQLHTTHSPHFLGLKTGHGLWNATNLASDVIVGVVDTGIWPEHVSFEDKGLPPVPRRWK 180
           LL LHTT+S  FLGL+ G GLWN T+L+SDVI+G+VDTGI PEHVSF D  + PVP RW+
Sbjct: 139 LLSLHTTYSHEFLGLEFGIGLWNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWR 198

Query: 181 GGCEEGQKFSASNCNRKLIGARAFIKAYEASFGRLNESGVFRSPRDWNGHGTHTASTAAG 240
           G C+EG  FS+S CN+K+IGA AF K YE+  G++NE+  FRS RD  GHGTHTASTAAG
Sbjct: 199 GSCDEGTNFSSSECNKKIIGASAFYKGYESIVGKINETTDFRSTRDAQGHGTHTASTAAG 258

Query: 241 NLVFKAGFYNHALGNAAGIRFTSRIAAYKVCWPLGCAIADVLAAMDRAVADGVDVLSLSL 300
           ++V KA ++  A G A+G+RFTSRIAAYK CW LGCA  DV+AA+DRA+ DGVDV+SLSL
Sbjct: 259 DIVPKANYFGQAKGLASGMRFTSRIAAYKACWALGCASTDVIAAIDRAILDGVDVISLSL 318

Query: 301 GGNPVPFYNDGLAIAAFGATQKGVFVSCAAGNSGPAPSTAGNLAPWIMTVGASYTDRIFP 360
           GG+  PFY D +AIA FGA QK +FVSC+AGNSGP  ST  N APW+MTV ASYTDR FP
Sbjct: 319 GGSSRPFYVDPIAIAGFGAMQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFP 378

Query: 361 TTVKLGNGQVFEGSSLFSGNNMGQLPLVYKQ---GRQGANLCLAGSLVPSMVRGKIVICE 420
             V++GN +   GSSL+ G ++  LPL + +      GA  C+  SL   +V GKIVIC 
Sbjct: 379 AIVRIGNRKSLVGSSLYKGKSLKNLPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVICL 438

Query: 421 RGTNTRIGKGEQVKLAGGAGMILINTQLEGEELFADPHVLPATSLGAAAGKAIKNYIASS 480
           RG + R  KGE+VK +GGA M+L++T+ EGEEL ADPHVLPA SLG + GK + NY+A +
Sbjct: 439 RGASGRTAKGEEVKRSGGAAMLLVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLAGA 498

Query: 481 KQPKASIAFQGTKYGSRAPRVAAFSSRGPSSIEPNIIKPDIIAPGVNILAAWPSIVSPSE 540
               AS+ F+GT YG+ AP VAAFSSRGPS   P I KPDI APG+NILA W    SPS 
Sbjct: 499 ANATASVRFRGTAYGATAPMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSL 558

Query: 541 LEFDKRRVLFNIVSGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTNDNKMSPI 600
           L  D RRV FNI+SGTSM+CPH+SG+AAL+K+ H DWSPA IKSA+MTTA   DN+  PI
Sbjct: 559 LRSDPRRVQFNIISGTSMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPI 618

Query: 601 SDVGSANGK-PANPFAFGSGHVDPEKASDPGLVYDITPEDYLNYLCSLNYNSTQIALVSR 660
            D G+A  +  A  FAFG+G+VDP +A DPGLVYD +  DYLNYLCSLNY S +I L S 
Sbjct: 619 GDRGAAGAESAATAFAFGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSG 678

Query: 661 GSFTCPSKRTFLQPGDLNYPSFSVFMKKKAKNVTVTLKRTVTNVGSPMSDYTVQINNPKG 720
            ++TC S    L PGDLNYPSF+V +   A   TV  KRTVTNVGSP  +Y V +  PKG
Sbjct: 679 TNYTCASNAVVLSPGDLNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKG 738

Query: 721 IKISVKPEKLSFGRLGEKLSYRVSFVAFGKEEALSEFSFGSLVWLSGKYVVKSPIAVTWQ 777
           +K+ V+P+ L F +  E+LSY V++ A     + S  SFG LVW+  KY V+SPIAVTW+
Sbjct: 739 VKVRVEPKVLKFQKARERLSYTVTYDAEASRNS-SSSSFGVLVWICDKYNVRSPIAVTWE 774

BLAST of Lag0041267 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 704.5 bits (1817), Expect = 9.3e-203
Identity = 382/780 (48.97%), Postives = 513/780 (65.77%), Query Frame = 0

Query: 7   WVLLCIVFA-ASTAALDQQTYIIHMDTTKMPTTNP--QQWYTSMIDSVNELASLDEDEEE 66
           ++LLC+ F   S+++ DQ TYI+HM  ++MP++      WY S + S             
Sbjct: 12  FLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRS------------- 71

Query: 67  VSNAAEIFYVYKNAISGKELKCVLDKYKNNGFAAKLSTKKLNSLSQTPGFLTAIPDQLLQ 126
           +S++AE+ Y Y+NAI              +GF+ +L+ ++ +SL   PG ++ +P+   +
Sbjct: 72  ISDSAELLYTYENAI--------------HGFSTRLTQEEADSLMTQPGVISVLPEHRYE 131

Query: 127 LHTTHSPHFLGL-KTGHGLWNATNLASDVIVGVVDTGIWPEHVSFEDKGLPPVPRRWKGG 186
           LHTT +P FLGL +    L+      SDV+VGV+DTG+WPE  S+ D+G  P+P  WKGG
Sbjct: 132 LHTTRTPLFLGLDEHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGG 191

Query: 187 CEEGQKFSASNCNRKLIGARAFIKAYEASFGRLNESGVFRSPRDWNGHGTHTASTAAGNL 246
           CE G  F+AS CNRKLIGAR F + YE++ G ++ES   RSPRD +GHGTHT+STAAG++
Sbjct: 192 CEAGTNFTASLCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSV 251

Query: 247 VFKAGFYNHALGNAAGIRFTSRIAAYKVCWPLGCAIADVLAAMDRAVADGVDVLSLSLGG 306
           V  A    +A G A G+   +R+A YKVCW  GC  +D+LAA+D+A+AD V+VLS+SLGG
Sbjct: 252 VEGASLLGYASGTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGG 311

Query: 307 NPVPFYNDGLAIAAFGATQKGVFVSCAAGNSGPAPSTAGNLAPWIMTVGASYTDRIFPTT 366
               +Y DG+AI AF A ++G+ VSC+AGN+GP+ S+  N+APWI TVGA   DR FP  
Sbjct: 312 GMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPAL 371

Query: 367 VKLGNGQVFEGSSLFSGNNMGQ--LPLVYKQGRQGA---NLCLAGSLVPSMVRGKIVICE 426
             LGNG+ F G SLF G  +    LP +Y      A   NLC+ G+L+P  V+GKIV+C+
Sbjct: 372 AILGNGKNFTGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCD 431

Query: 427 RGTNTRIGKGEQVKLAGGAGMILINTQLEGEELFADPHVLPATSLGAAAGKAIKNYIASS 486
           RG N R+ KG+ VK AGG GMIL NT   GEEL AD H+LPAT++G  AG  I++Y+ + 
Sbjct: 432 RGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTD 491

Query: 487 KQPKASIAFQGTKYGSR-APRVAAFSSRGPSSIEPNIIKPDIIAPGVNILAAWPSIVSPS 546
             P ASI+  GT  G + +P VAAFSSRGP+SI PNI+KPD+IAPGVNILAAW     P+
Sbjct: 492 PNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPT 551

Query: 547 ELEFDKRRVLFNIVSGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTNDNKMSP 606
            L  D RRV FNI+SGTSMSCPHVSGLAALLK+ H +WSPAAI+SALMTTAY       P
Sbjct: 552 GLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKP 611

Query: 607 ISDVGSANGKPANPFAFGSGHVDPEKASDPGLVYDITPEDYLNYLCSLNYNSTQIALVSR 666
           + D+  A GKP+ PF  G+GHV P  A++PGL+YD+T EDYL +LC+LNY S QI  VSR
Sbjct: 612 LLDI--ATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSR 671

Query: 667 GSFTCPSKRTFLQPGDLNYPSFSVFMKKKAKNVTVTLKRTVTNVGSPMSDYTVQI-NNPK 726
            ++TC   +++    DLNYPSF+V +            RTVT+VG     Y+V++ +   
Sbjct: 672 RNYTCDPSKSY-SVADLNYPSFAVNVDGVG---AYKYTRTVTSVGG-AGTYSVKVTSETT 731

Query: 727 GIKISVKPEKLSFGRLGEKLSYRVSFVAFGKEEALSEFSFGSLVWLSGKYVVKSPIAVTW 776
           G+KISV+P  L+F    EK SY V+F     + + S  SFGS+ W  GK+VV SP+A++W
Sbjct: 732 GVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSN-SFGSIEWSDGKHVVGSPVAISW 756

BLAST of Lag0041267 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 689.9 bits (1779), Expect = 2.4e-198
Identity = 383/784 (48.85%), Postives = 499/784 (63.65%), Query Frame = 0

Query: 15  AASTAALDQQTYIIHMDTTKMPTTNPQ--QWYTSMIDSVNELASLDEDEEEVSNAAEIFY 74
           ++S ++ +  TYI+H+D    P+  P    WYTS       LASL       S+   I +
Sbjct: 17  SSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTS------SLASL------TSSPPSIIH 76

Query: 75  VYKNAISGKELKCVLDKYKNNGFAAKLSTKKLNSLSQTPGFLTAIPDQLLQLHTTHSPHF 134
            Y                  +GF+A+L+++  + L   P  ++ IP+Q+  LHTT SP F
Sbjct: 77  TYDTVF--------------HGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEF 136

Query: 135 LGLKTGH--GLWNATNLASDVIVGVVDTGIWPEHVSFEDKGLPPVPRRWKGGCEEGQKFS 194
           LGL++    GL   ++  SD+++GV+DTG+WPE  SF+D+GL PVP +WKG C   Q F 
Sbjct: 137 LGLRSTDKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFP 196

Query: 195 ASNCNRKLIGARAFIKAYEASFGRLNESGVFRSPRDWNGHGTHTASTAAGNLVFKAGFYN 254
            S CNRKL+GAR F   YEA+ G++NE+  FRSPRD +GHGTHTAS +AG  VF A    
Sbjct: 197 ESACNRKLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLG 256

Query: 255 HALGNAAGIRFTSRIAAYKVCWPLGCAIADVLAAMDRAVADGVDVLSLSLGGNPVPFYND 314
           +A G AAG+   +R+AAYKVCW  GC  +D+LAA D AVADGVDV+SLS+GG  VP+Y D
Sbjct: 257 YAHGVAAGMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLD 316

Query: 315 GLAIAAFGATQKGVFVSCAAGNSGPAPSTAGNLAPWIMTVGASYTDRIFPTTVKLGNGQV 374
            +AI AFGA  +G+FVS +AGN GP   T  N+APW+ TVGA   DR FP  VKLGNG++
Sbjct: 317 AIAIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKM 376

Query: 375 FEGSSLFSGNNMG---QLPLVYKQGRQG-----ANLCLAGSLVPSMVRGKIVICERGTNT 434
             G S++ G  +      PLVY     G     ++LCL GSL P++V+GKIV+C+RG N+
Sbjct: 377 ISGVSVYGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINS 436

Query: 435 RIGKGEQVKLAGGAGMILINTQLEGEELFADPHVLPATSLGAAAGKAIKNYIA------S 494
           R  KGE V+  GG GMI+ N   +GE L AD HVLPATS+GA+ G  I+ YI+      S
Sbjct: 437 RATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRS 496

Query: 495 SKQPKASIAFQGTKYGSR-APRVAAFSSRGPSSIEPNIIKPDIIAPGVNILAAWPSIVSP 554
           SK P A+I F+GT+ G R AP VA+FS+RGP+   P I+KPD+IAPG+NILAAWP  + P
Sbjct: 497 SKHPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGP 556

Query: 555 SELEFDKRRVLFNIVSGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTNDNKMS 614
           S +  D RR  FNI+SGTSM+CPHVSGLAALLKAAH DWSPAAI+SAL+TTAYT DN   
Sbjct: 557 SGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGE 616

Query: 615 PISDVGSANGKPANPFAFGSGHVDPEKASDPGLVYDITPEDYLNYLCSLNYNSTQIALVS 674
           P+ D   + G  ++   +GSGHV P KA DPGLVYDIT  DY+N+LC+ NY  T I  ++
Sbjct: 617 PMMD--ESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTIT 676

Query: 675 RGSFTCPSKRTFLQPGDLNYPSFS-VFMKKKAKNVTVTLKRTVTNVGSPMSDYTVQINNP 734
           R    C   R     G+LNYPSFS VF +     ++    RTVTNVG   S Y ++I  P
Sbjct: 677 RRQADCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPP 736

Query: 735 KGIKISVKPEKLSFGRLGEKLSY--RVSFVAFGKEEALSEFSFGSLVWLSGKYVVKSPIA 777
           +G  ++V+PEKLSF R+G+KLS+  RV           +    G +VW  GK  V SP+ 
Sbjct: 737 RGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLV 772

BLAST of Lag0041267 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 689.9 bits (1779), Expect = 2.4e-198
Identity = 382/784 (48.72%), Postives = 504/784 (64.29%), Query Frame = 0

Query: 8   VLLCIVFAASTAALDQQTYIIHMDTTKMPT--TNPQQWYTSMIDSVNELASLDEDEEEVS 67
           +LLC    +S+++   ++YI+H+  +  P+  ++   W+ S++ S+              
Sbjct: 15  LLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSL----------PSSP 74

Query: 68  NAAEIFYVYKNAISGKELKCVLDKYKNNGFAAKLSTKKLNSLSQTPGFLTAIPDQLLQLH 127
             A + Y Y  A+              +GF+A+LS  +  +L + P  ++ IPDQ  ++H
Sbjct: 75  QPATLLYSYSRAV--------------HGFSARLSPIQTAALRRHPSVISVIPDQAREIH 134

Query: 128 TTHSPHFLGLKTGHGLWNATNLASDVIVGVVDTGIWPEHVSFEDKGLPPVPRRWKGGCEE 187
           TTH+P FLG     GLW+ +N   DVIVGV+DTGIWPEH SF D GL P+P  WKG CE 
Sbjct: 135 TTHTPAFLGFSQNSGLWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEI 194

Query: 188 GQKFSASNCNRKLIGARAFIKAY--EASFGRLNESGVFRSPRDWNGHGTHTASTAAGNLV 247
           G  F AS+CNRKLIGARAF + Y  + +  + + +   RSPRD  GHGTHTASTAAG++V
Sbjct: 195 GPDFPASSCNRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVV 254

Query: 248 FKAGFYNHALGNAAGIRFTSRIAAYKVCWPLGCAIADVLAAMDRAVADGVDVLSLSLG-- 307
             A  Y +A G A G+   +RIAAYK+CW  GC  +D+LAAMD+AVADGV V+SLS+G  
Sbjct: 255 ANASLYQYARGTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGAS 314

Query: 308 GNPVPFYNDGLAIAAFGATQKGVFVSCAAGNSGPAPSTAGNLAPWIMTVGASYTDRIFPT 367
           G+   ++ D +AI AFGAT+ G+ VSC+AGNSGP P TA N+APWI+TVGAS  DR F  
Sbjct: 315 GSAPEYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAA 374

Query: 368 TVKLGNGQVFEGSSLFSGNNM--GQLPLVYKQGRQGANLCLAGSLVPSMVRGKIVICERG 427
               G+G+VF G+SL++G ++   QL LVY  G  G+ LC  G L  S+V GKIV+C+RG
Sbjct: 375 NAITGDGKVFTGTSLYAGESLPDSQLSLVY-SGDCGSRLCYPGKLNSSLVEGKIVLCDRG 434

Query: 428 TNTRIGKGEQVKLAGGAGMILINTQLEGEELFADPHVLPATSLGAAAGKAIKNYIASSKQ 487
            N R+ KG  VKLAGGAGMIL NT   GEEL AD H++PAT +GA AG  I++YI +S  
Sbjct: 435 GNARVEKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDS 494

Query: 488 PKASIAFQGTKYGSR--APRVAAFSSRGPSSIEPNIIKPDIIAPGVNILAAWPSIVSPSE 547
           P A I+F GT  G    +PRVAAFSSRGP+ + P I+KPD+IAPGVNILA W  +V P++
Sbjct: 495 PTAKISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTD 554

Query: 548 LEFDKRRVLFNIVSGTSMSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTNDNKMSPI 607
           L+ D RRV FNI+SGTSMSCPHVSGLAALL+ AH DWSPAAIKSAL+TTAY  +N   PI
Sbjct: 555 LDIDPRRVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPI 614

Query: 608 SDVGSANGKPANPFAFGSGHVDPEKASDPGLVYDITPEDYLNYLCSLNYNSTQIALVSRG 667
            D+  A GK +N F  G+GHVDP KA +PGLVYDI  ++Y+ +LC++ Y    I +  + 
Sbjct: 615 EDL--ATGKSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQD 674

Query: 668 SFTCPSKRT--FLQPGDLNYPSFSVFMKKKAKNVTVTLKRTVTNVGSPM-SDYTVQINNP 727
                +  T      GDLNYPSFSV      +   V  KR V NVGS + + Y V + +P
Sbjct: 675 PTLYDACETSKLRTAGDLNYPSFSVVFASTGE--VVKYKRVVKNVGSNVDAVYEVGVKSP 734

Query: 728 KGIKISVKPEKLSFGRLGEKLSYRVSF---VAFGKEEALSEFSFGSLVWLSGKYVVKSPI 776
             ++I V P KL+F +    L Y V+F   V  G   ++    FGS+ W  G++VVKSP+
Sbjct: 735 ANVEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPV 769

BLAST of Lag0041267 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 676.0 bits (1743), Expect = 3.6e-194
Identity = 367/762 (48.16%), Postives = 484/762 (63.52%), Query Frame = 0

Query: 23  QQTYIIHMDTTKMPTT--NPQQWYTSMIDSVNELASLDEDEEEVSNAAEIFYVYKNAISG 82
           ++TYII ++ +  P +      WYTS ++S + L                 Y Y  +   
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSESSL----------------LYTYTTSF-- 86

Query: 83  KELKCVLDKYKNNGFAAKLSTKKLNS-LSQTPGFLTAIPDQLLQLHTTHSPHFLGLKTGH 142
                       +GF+A L + + +S LS +   L    D L  LHTT +P FLGL +  
Sbjct: 87  ------------HGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEF 146

Query: 143 GLWNATNLASDVIVGVVDTGIWPEHVSFEDKGLPPVPRRWKGGCEEGQKFSASNCNRKLI 202
           G+ +  + ++ VI+GV+DTG+WPE  SF+D  +P +P +WKG CE G  F +  CN+KLI
Sbjct: 147 GVHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLI 206

Query: 203 GARAFIKAYE-ASFGRLNESGVFRSPRDWNGHGTHTASTAAGNLVFKAGFYNHALGNAAG 262
           GAR+F K ++ AS G  +      SPRD +GHGTHT++TAAG+ V  A F  +A G A G
Sbjct: 207 GARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARG 266

Query: 263 IRFTSRIAAYKVCWPLGCAIADVLAAMDRAVADGVDVLSLSLGGNPVPFYNDGLAIAAFG 322
           +   +R+A YKVCW  GC  +D+LAAMDRA+ DGVDVLSLSLGG   P+Y D +AI AF 
Sbjct: 267 MATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFS 326

Query: 323 ATQKGVFVSCAAGNSGPAPSTAGNLAPWIMTVGASYTDRIFPTTVKLGNGQVFEGSSLFS 382
           A ++GVFVSC+AGNSGP  ++  N+APW+MTVGA   DR FP    LGNG+   G SL+S
Sbjct: 327 AMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYS 386

Query: 383 GNNMGQLP--LVYKQGR-QGANLCLAGSLVPSMVRGKIVICERGTNTRIGKGEQVKLAGG 442
           G  MG  P  LVY +G    +NLCL GSL  S+VRGKIV+C+RG N R+ KG  V+ AGG
Sbjct: 387 GVGMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGG 446

Query: 443 AGMILINTQLEGEELFADPHVLPATSLGAAAGKAIKNYIASSKQPKASIAFQGTKYGSR- 502
            GMI+ NT   GEEL AD H+LPA ++G   G  ++ Y+ S  +P A + F+GT    + 
Sbjct: 447 LGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKP 506

Query: 503 APRVAAFSSRGPSSIEPNIIKPDIIAPGVNILAAWPSIVSPSELEFDKRRVLFNIVSGTS 562
           +P VAAFSSRGP+++ P I+KPD+I PGVNILA W   + P+ L+ D RR  FNI+SGTS
Sbjct: 507 SPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTS 566

Query: 563 MSCPHVSGLAALLKAAHKDWSPAAIKSALMTTAYTNDNKMSPISDVGSANGKPANPFAFG 622
           MSCPH+SGLA LLKAAH +WSP+AIKSALMTTAY  DN  +P+ D  +A+   +NP+A G
Sbjct: 567 MSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHD--AADNSLSNPYAHG 626

Query: 623 SGHVDPEKASDPGLVYDITPEDYLNYLCSLNYNSTQI-ALVSRGSFTCPSKRTFLQPGDL 682
           SGHVDP+KA  PGLVYDI+ E+Y+ +LCSL+Y    I A+V R S  C  K  F  PG L
Sbjct: 627 SGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKK--FSDPGQL 686

Query: 683 NYPSFSVFMKKKAKNVTVTLKRTVTNVGSPMSDYTVQINNPKGIKISVKPEKLSFGRLGE 742
           NYPSFSV    K     V   R VTNVG+  S Y V +N    + ISVKP KLSF  +GE
Sbjct: 687 NYPSFSVLFGGKR---VVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGE 746

Query: 743 KLSYRVSFVAFGKEEALSEFSFGSLVWLSGKYVVKSPIAVTW 776
           K  Y V+FV+       ++  FGS+ W + ++ V+SP+A +W
Sbjct: 747 KKRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSW 751