Lag0041261 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0041261 vs. NCBI nr
Match: XP_022989707.1 (subtilisin-like protease SBT1.1 [Cucurbita maxima] >XP_022989715.1 subtilisin-like protease SBT1.1 [Cucurbita maxima])

HSP 1 Score: 1290.4 bits (3338), Expect = 0.0e+00
Identity = 642/766 (83.81%), Postives = 698/766 (91.12%), Query Frame = 0

Query: 16  MGFREVW-VLLSIMLATSTAAVDQQTYIIHMDTTKMATTNPEQWYMDMIDSVNKISSL-- 75
           MG  E+W +LLSIMLATS AAVDQQTYIIHMD TKMATTNPEQWY  +I S+N++SS+  
Sbjct: 1   MGITELWLLLLSIMLATSAAAVDQQTYIIHMDATKMATTNPEQWYTSIIHSINQLSSIND 60

Query: 76  DEEEASNAAEILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFL 135
           D+ EASNAAEILY+YKTAISGF+AKLSTRKLHSLSK+PGFLAATPD+LLQLHTTH+PQFL
Sbjct: 61  DQNEASNAAEILYIYKTAISGFSAKLSTRKLHSLSKLPGFLAATPDKLLQLHTTHTPQFL 120

Query: 136 GLQRGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPKKWKGICQAGPKFSRSN 195
           GLQRGHGLWN+SNLASDIIIGV+DTGIWPEHISFQDKGLPPVPKKWKG CQAGPKFSRSN
Sbjct: 121 GLQRGHGLWNASNLASDIIIGVIDTGIWPEHISFQDKGLPPVPKKWKGTCQAGPKFSRSN 180

Query: 196 CNKKLVGARAYIKGYETIVGKLNATGTFRSPRDSDGHGTHTASTAAGNIVYRASLYNQGM 255
           CNKKLVGARAYIKGYETI+G+LN TGTFRS RDSDGHGTHTASTAAGNIVY+ASLYNQGM
Sbjct: 181 CNKKLVGARAYIKGYETILGRLNTTGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQGM 240

Query: 256 GVATGMGFNSRIAAYKVCWQEGCANTDILAAIDRAVADGVDVLSLSLGGGAGFFDRDGIA 315
           G ATGM F SRIAAYKVCW EGCA+TDILAAIDRAV DGVDVLSLSLGGG GFF +D IA
Sbjct: 241 GAATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVVDGVDVLSLSLGGGDGFFYQDEIA 300

Query: 316 IAAFGAVRNGVFVSCSAGNSGPYISTVSNVAPWIMTVAASYTDRTFPAIVKLGNGKVFEG 375
           IAAFGAVRNGVFVSCSAGNSGP++STV NVAPWIMTVAASYTDRTFP  VKLGNG++FEG
Sbjct: 301 IAAFGAVRNGVFVSCSAGNSGPFMSTVGNVAPWIMTVAASYTDRTFPGSVKLGNGQIFEG 360

Query: 376 SSLYSGKNINQLPLVYNNTAGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTEKGEQVK 435
           SSL+SG +I +LPLVYN TAGG+EA+VCTAGSLVPS VKGKIVVCERGTNSR EKGEQVK
Sbjct: 361 SSLHSGNSIGELPLVYNKTAGGEEASVCTAGSLVPSMVKGKIVVCERGTNSRFEKGEQVK 420

Query: 436 LAGGAGMIVINTELEGEELLADLHVLPATALGFSAGKAIINYIASSKHQPRASITFEGTK 495
           LAGG GMI+INT+LEGEEL  D HVLPA  LG SAGKAIINYIASSKH P+ASI FEGT+
Sbjct: 421 LAGGVGMILINTQLEGEELFGDSHVLPAINLGASAGKAIINYIASSKHPPKASILFEGTR 480

Query: 496 FGSQAPRVAAFSSRGPTLFKPYVIKPDITAPGVNILAAWPPITSPTELESDKRRVLFNII 555
           +GS+APR+AAFSSRGP+ F+PYVIKPDITAPGVNILAAWPP+ SP+EL+SDKRRVLFNII
Sbjct: 481 YGSRAPRMAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPPVVSPSELKSDKRRVLFNII 540

Query: 556 SGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYITDNKMSLIPDAGRASGRPADP 615
           SGTSMSCPHVSG+AALLKSAHK+WSPAAIKSALMTTAY+ DNK SLI D GR SG PADP
Sbjct: 541 SGTSMSCPHVSGIAALLKSAHKNWSPAAIKSALMTTAYVNDNKRSLISDVGRPSGGPADP 600

Query: 616 FAYGSGHVDPEKASDPGLIYDIAPQDYLKYLCSLNYTSSQVALVSRGNFACPSKRKLLKP 675
           +A+GSGHVDPEKA DPGL+YDI PQDYL YLCSLNYTS QV LVSRGNF+CPSKR +L+P
Sbjct: 601 YAFGSGHVDPEKAVDPGLVYDIVPQDYLNYLCSLNYTSKQVGLVSRGNFSCPSKRTVLQP 660

Query: 676 GDLNYPSFSVLMKKKAKNVIVTFKRTVTNVGSPSSDYTIIVNNPKGVRVIVKPEMLSFRR 735
           GDLNYPSFSV MK KAKN  V FKRTVTNVG+PSSDYT+ +NNP G+RV VKPE LSFRR
Sbjct: 661 GDLNYPSFSVSMKNKAKN--VRFKRTVTNVGTPSSDYTVKINNPSGIRVSVKPEKLSFRR 720

Query: 736 LGQKLSYQVSFVALGKGEALSDFSFGSLIWFSGKYVVRSPIAVTWQ 779
            GQKLSYQVSFVALGK E LSDFSFGSL+W SGKY VRSPIAV W+
Sbjct: 721 SGQKLSYQVSFVALGKREGLSDFSFGSLVWVSGKYSVRSPIAVNWE 764

BLAST of Lag0041261 vs. NCBI nr
Match: KAG6599889.1 (Subtilisin-like protease 1.1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1271.5 bits (3289), Expect = 0.0e+00
Identity = 630/766 (82.25%), Postives = 694/766 (90.60%), Query Frame = 0

Query: 16  MGFREVWVLLSIMLATS-TAAVDQQTYIIHMDTTKMATTNPEQWYMDMIDSVNKISSL-- 75
           MG  E+W+LLSIMLATS  AA DQQ+YIIHMD TKMATTNP+QWY  +ID++N++SS+  
Sbjct: 1   MGITELWLLLSIMLATSAAAAADQQSYIIHMDATKMATTNPQQWYTSIIDTINQLSSIND 60

Query: 76  DEEEASNAAEILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFL 135
           D+ EASNAAEILY+YKTAISGF+AKLSTRKLHSLSK+PGFL+ATPD+LLQLHTTH+P+FL
Sbjct: 61  DQNEASNAAEILYIYKTAISGFSAKLSTRKLHSLSKLPGFLSATPDKLLQLHTTHTPKFL 120

Query: 136 GLQRGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPKKWKGICQAGPKFSRSN 195
           GLQRGHGLWN+SNLASDIIIGV+DTGIWPEHISFQDKGLP VPKKWKG CQAGPKFSRSN
Sbjct: 121 GLQRGHGLWNASNLASDIIIGVIDTGIWPEHISFQDKGLPAVPKKWKGTCQAGPKFSRSN 180

Query: 196 CNKKLVGARAYIKGYETIVGKLNATGTFRSPRDSDGHGTHTASTAAGNIVYRASLYNQGM 255
           CNKKL+GA AYIKGYETI+G+LN TGTFRS RDSDGHGTHTASTAAGNIVY+ASLYNQGM
Sbjct: 181 CNKKLIGATAYIKGYETILGRLNTTGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQGM 240

Query: 256 GVATGMGFNSRIAAYKVCWQEGCANTDILAAIDRAVADGVDVLSLSLGGGAGFFDRDGIA 315
           G ATGM F SRIAAYKVCW EGCA+TDILAAIDRAV DGVDVLSLSLGGG GFF +D IA
Sbjct: 241 GAATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVVDGVDVLSLSLGGGDGFFYQDEIA 300

Query: 316 IAAFGAVRNGVFVSCSAGNSGPYISTVSNVAPWIMTVAASYTDRTFPAIVKLGNGKVFEG 375
           IAAFGAVRNGVFVSCSAGNSGP++STV NVAPWIMTVAASYTDRTF   VKLGNG++FEG
Sbjct: 301 IAAFGAVRNGVFVSCSAGNSGPFMSTVGNVAPWIMTVAASYTDRTFAGSVKLGNGQIFEG 360

Query: 376 SSLYSGKNINQLPLVYNNTAGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTEKGEQVK 435
           SSL+SG +I QLPLVYN TAGG+EANVCTAGSLVPS VKGKIVVCERGTNSR EKGEQVK
Sbjct: 361 SSLHSGNSIGQLPLVYNKTAGGEEANVCTAGSLVPSMVKGKIVVCERGTNSRFEKGEQVK 420

Query: 436 LAGGAGMIVINTELEGEELLADLHVLPATALGFSAGKAIINYIASSKHQPRASITFEGTK 495
           LAGG GMI+INT+LEGEEL AD HVLPA  LG SAGKAIINYIASSK  P+ASI FEGT+
Sbjct: 421 LAGGVGMILINTQLEGEELFADSHVLPAVNLGASAGKAIINYIASSKQPPKASILFEGTR 480

Query: 496 FGSQAPRVAAFSSRGPTLFKPYVIKPDITAPGVNILAAWPPITSPTELESDKRRVLFNII 555
           +GS+APR+AAFSSRGP+ F+PYVIKPDITAPGVNILAAWPP+ SP+EL+SDKRRVLFNII
Sbjct: 481 YGSRAPRMAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPPVVSPSELKSDKRRVLFNII 540

Query: 556 SGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYITDNKMSLIPDAGRASGRPADP 615
           SGTSMSCPHVSG+AALLKSAH +WSPAAIKSALMTTAY+ DNK S+I D GR SG PADP
Sbjct: 541 SGTSMSCPHVSGIAALLKSAHNNWSPAAIKSALMTTAYVNDNKRSVISDVGRPSGGPADP 600

Query: 616 FAYGSGHVDPEKASDPGLIYDIAPQDYLKYLCSLNYTSSQVALVSRGNFACPSKRKLLKP 675
           +A+GSGHVDPEKA DPGL+YDI PQDYL YLCSLNYTS QV LVSRGNF+CPSKR L++P
Sbjct: 601 YAFGSGHVDPEKAVDPGLVYDIEPQDYLNYLCSLNYTSKQVGLVSRGNFSCPSKRNLVQP 660

Query: 676 GDLNYPSFSVLMKKKAKNVIVTFKRTVTNVGSPSSDYTIIVNNPKGVRVIVKPEMLSFRR 735
           GDLNYPSFSV MK +AKN  VTFKRTVTNVG+P+SDYT+ +NNP G+RV VKP+ LSFRR
Sbjct: 661 GDLNYPSFSVSMKNRAKN--VTFKRTVTNVGTPTSDYTVKINNPSGIRVSVKPKKLSFRR 720

Query: 736 LGQKLSYQVSFVALGKGEALSDFSFGSLIWFSGKYVVRSPIAVTWQ 779
            GQKL YQVSFVALGK E LSDFSFGSL+W SGKY VRSPIAV W+
Sbjct: 721 SGQKLRYQVSFVALGKREGLSDFSFGSLVWVSGKYSVRSPIAVNWE 764

BLAST of Lag0041261 vs. NCBI nr
Match: XP_022943077.1 (subtilisin-like protease SBT1.1 [Cucurbita moschata] >XP_022943079.1 subtilisin-like protease SBT1.1 [Cucurbita moschata])

HSP 1 Score: 1269.6 bits (3284), Expect = 0.0e+00
Identity = 629/766 (82.11%), Postives = 693/766 (90.47%), Query Frame = 0

Query: 16  MGFREVWVLLSIMLATS-TAAVDQQTYIIHMDTTKMATTNPEQWYMDMIDSVNKISSL-- 75
           MG  E+W+LLSIMLATS  AA DQQ+YIIHMD TKMATTNP+QWY  +ID++N++SS+  
Sbjct: 1   MGITELWLLLSIMLATSAAAAADQQSYIIHMDATKMATTNPQQWYTSIIDTINQLSSIND 60

Query: 76  DEEEASNAAEILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFL 135
           D+ EASNAAEILY+YKTAISGF+AKLSTRKLHSLSK+PGFL+ATPD+LLQLHTTH+P+FL
Sbjct: 61  DQNEASNAAEILYIYKTAISGFSAKLSTRKLHSLSKLPGFLSATPDKLLQLHTTHTPKFL 120

Query: 136 GLQRGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPKKWKGICQAGPKFSRSN 195
           GLQRGHGLWN+SNLASDIIIGV+DTGIWPEHISFQDKGLP VPKKWKG CQAGPKFSRSN
Sbjct: 121 GLQRGHGLWNASNLASDIIIGVIDTGIWPEHISFQDKGLPAVPKKWKGTCQAGPKFSRSN 180

Query: 196 CNKKLVGARAYIKGYETIVGKLNATGTFRSPRDSDGHGTHTASTAAGNIVYRASLYNQGM 255
           CNKKL+GA AYIKGYETI+G+LN TGTFRS RDSDGHGTHTASTAAGNIVY+ASLYNQGM
Sbjct: 181 CNKKLIGATAYIKGYETILGRLNTTGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQGM 240

Query: 256 GVATGMGFNSRIAAYKVCWQEGCANTDILAAIDRAVADGVDVLSLSLGGGAGFFDRDGIA 315
           G ATGM F SRIAAYKVCW EGCA+TDILAAIDRAV DGVDVLSLSLGGG GFF +D IA
Sbjct: 241 GAATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVVDGVDVLSLSLGGGDGFFYQDEIA 300

Query: 316 IAAFGAVRNGVFVSCSAGNSGPYISTVSNVAPWIMTVAASYTDRTFPAIVKLGNGKVFEG 375
           IAAFGAVRNGVFVSCSAGNSGP++STV NVAPWIMTVAASYTDRTF   VKLGNG++FEG
Sbjct: 301 IAAFGAVRNGVFVSCSAGNSGPFMSTVGNVAPWIMTVAASYTDRTFAGSVKLGNGQIFEG 360

Query: 376 SSLYSGKNINQLPLVYNNTAGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTEKGEQVK 435
           SSL+SG +I QLPLVYN TAGG+EANVCTAGSLVPS VKGKIVVCERGTNSR EKGEQVK
Sbjct: 361 SSLHSGNSIGQLPLVYNKTAGGEEANVCTAGSLVPSMVKGKIVVCERGTNSRFEKGEQVK 420

Query: 436 LAGGAGMIVINTELEGEELLADLHVLPATALGFSAGKAIINYIASSKHQPRASITFEGTK 495
           LAGG GMI+INT+LEGEEL AD HVLPA  LG SAGKAIINYIASSK  P+ASI FEGT+
Sbjct: 421 LAGGVGMILINTQLEGEELFADSHVLPAVNLGASAGKAIINYIASSKQPPKASILFEGTR 480

Query: 496 FGSQAPRVAAFSSRGPTLFKPYVIKPDITAPGVNILAAWPPITSPTELESDKRRVLFNII 555
           +GS+APR+AAFSSRGP+ F+PYVIKPDITAPGVNILAAWPP+ SP+EL+SDKRRVLFNII
Sbjct: 481 YGSRAPRMAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPPVVSPSELKSDKRRVLFNII 540

Query: 556 SGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYITDNKMSLIPDAGRASGRPADP 615
           SGTSMSCPHVSG+AALLKSAH +WSPAAIKSALMTTAY+ DNK S+I D GR SG PADP
Sbjct: 541 SGTSMSCPHVSGIAALLKSAHNNWSPAAIKSALMTTAYVNDNKRSVISDVGRPSGGPADP 600

Query: 616 FAYGSGHVDPEKASDPGLIYDIAPQDYLKYLCSLNYTSSQVALVSRGNFACPSKRKLLKP 675
           +A+GSGHVDPEKA DPGL+YDI PQDYL YLCSLNYTS QV LVSRGNF+CPS R L++P
Sbjct: 601 YAFGSGHVDPEKAVDPGLVYDIEPQDYLNYLCSLNYTSKQVGLVSRGNFSCPSNRNLVQP 660

Query: 676 GDLNYPSFSVLMKKKAKNVIVTFKRTVTNVGSPSSDYTIIVNNPKGVRVIVKPEMLSFRR 735
           GDLNYPSFSV MK +AKN  VTFKRTVTNVG+P+SDYT+ +NNP G+RV VKP+ LSFRR
Sbjct: 661 GDLNYPSFSVSMKNRAKN--VTFKRTVTNVGTPTSDYTVKINNPSGIRVSVKPKKLSFRR 720

Query: 736 LGQKLSYQVSFVALGKGEALSDFSFGSLIWFSGKYVVRSPIAVTWQ 779
            GQKL YQVSFVALGK E LSDFSFGSL+W SGKY VRSPIAV W+
Sbjct: 721 SGQKLRYQVSFVALGKREGLSDFSFGSLVWVSGKYSVRSPIAVNWE 764

BLAST of Lag0041261 vs. NCBI nr
Match: XP_023544752.1 (subtilisin-like protease SBT1.1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1267.3 bits (3278), Expect = 0.0e+00
Identity = 629/765 (82.22%), Postives = 693/765 (90.59%), Query Frame = 0

Query: 16  MGFREVWVLLSIMLATSTAAVDQQTYIIHMDTTKMATTNPEQWYMDMIDSVNKISSL--D 75
           MG  E+W+LLSIMLATS AAVDQQ+YIIHMDTTKMATTNP+QWY  +ID+VN++SS+  D
Sbjct: 1   MGITELWLLLSIMLATSAAAVDQQSYIIHMDTTKMATTNPQQWYTSIIDTVNQLSSINDD 60

Query: 76  EEEASNAAEILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFLG 135
           + EASNAAEILY+YKTAISGF+AKLSTRKL SLSK+PGFL+ATPD+LLQLHTTH+P+FLG
Sbjct: 61  QNEASNAAEILYIYKTAISGFSAKLSTRKLLSLSKLPGFLSATPDKLLQLHTTHTPKFLG 120

Query: 136 LQRGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPKKWKGICQAGPKFSRSNC 195
           LQRGHGLWN+S LASDIIIGV+DTGIWPEHISFQDKGL PVPKKWKG CQAGPKFSRSNC
Sbjct: 121 LQRGHGLWNASYLASDIIIGVIDTGIWPEHISFQDKGLSPVPKKWKGTCQAGPKFSRSNC 180

Query: 196 NKKLVGARAYIKGYETIVGKLNATGTFRSPRDSDGHGTHTASTAAGNIVYRASLYNQGMG 255
           N KL+GARAYIKGYETI+G+LN TGTFRS RDSDGHGTHTASTAAGNIVY+ASLYNQGMG
Sbjct: 181 NNKLIGARAYIKGYETILGRLNTTGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQGMG 240

Query: 256 VATGMGFNSRIAAYKVCWQEGCANTDILAAIDRAVADGVDVLSLSLGGGAGFFDRDGIAI 315
            ATGM F SRIAAYKVCW EGCA+TDILAAIDRAV DGVDVLSLSLGGG GFF +D IAI
Sbjct: 241 AATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVVDGVDVLSLSLGGGDGFFYQDEIAI 300

Query: 316 AAFGAVRNGVFVSCSAGNSGPYISTVSNVAPWIMTVAASYTDRTFPAIVKLGNGKVFEGS 375
           AAFGAVRNGVFVSCSAGNSGP++STV NVAPWIMTVAASYTDRTF   VKLGNG++FEGS
Sbjct: 301 AAFGAVRNGVFVSCSAGNSGPFMSTVGNVAPWIMTVAASYTDRTFAGSVKLGNGQIFEGS 360

Query: 376 SLYSGKNINQLPLVYNNTAGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTEKGEQVKL 435
           SL+SG +I QLPLVYN TAGG+EANVCTAGSLVPS VKGKIVVCERGTNSR EKGEQVKL
Sbjct: 361 SLHSGNSIGQLPLVYNKTAGGEEANVCTAGSLVPSMVKGKIVVCERGTNSRFEKGEQVKL 420

Query: 436 AGGAGMIVINTELEGEELLADLHVLPATALGFSAGKAIINYIASSKHQPRASITFEGTKF 495
           AGG GMI+INT+LEGEEL AD HVLPA  LG SAGKAI NYIASSK  P+ASI FEGT++
Sbjct: 421 AGGVGMILINTQLEGEELFADSHVLPAVNLGASAGKAITNYIASSKQPPKASILFEGTRY 480

Query: 496 GSQAPRVAAFSSRGPTLFKPYVIKPDITAPGVNILAAWPPITSPTELESDKRRVLFNIIS 555
           GS+APR+AAFSSRGP+ F+PYVIKPDITAPGVNILAAWPP+ SP+EL+SD+RRVLFNIIS
Sbjct: 481 GSRAPRMAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPPVVSPSELKSDERRVLFNIIS 540

Query: 556 GTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYITDNKMSLIPDAGRASGRPADPF 615
           GTSMSCPHVSG+AALLKSAHK+WSPAAIKSALMTTAY+TDNK S+I D GR SG PADP+
Sbjct: 541 GTSMSCPHVSGIAALLKSAHKNWSPAAIKSALMTTAYVTDNKRSVISDVGRPSGGPADPY 600

Query: 616 AYGSGHVDPEKASDPGLIYDIAPQDYLKYLCSLNYTSSQVALVSRGNFACPSKRKLLKPG 675
           A+GSGHVDPEKA DPGL+YDIAPQDYL YLCSLNYTS QV LVSRGNF+CPSK  +L+PG
Sbjct: 601 AFGSGHVDPEKAVDPGLVYDIAPQDYLNYLCSLNYTSKQVGLVSRGNFSCPSKGTVLQPG 660

Query: 676 DLNYPSFSVLMKKKAKNVIVTFKRTVTNVGSPSSDYTIIVNNPKGVRVIVKPEMLSFRRL 735
           DLNYPSFSV MK +AKN  VT KRTVTNVG+P+SDYT+ +NNP G+ V VKP+ LSFRR 
Sbjct: 661 DLNYPSFSVSMKNRAKN--VTLKRTVTNVGTPTSDYTVKINNPSGIAVSVKPKKLSFRRS 720

Query: 736 GQKLSYQVSFVALGKGEALSDFSFGSLIWFSGKYVVRSPIAVTWQ 779
           GQKLSYQVSFVA GK E LSDFSFGSL+W SGKY VRSPIAV W+
Sbjct: 721 GQKLSYQVSFVASGKREGLSDFSFGSLVWVSGKYSVRSPIAVNWE 763

BLAST of Lag0041261 vs. NCBI nr
Match: XP_023544740.1 (subtilisin-like protease SBT1.1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1263.8 bits (3269), Expect = 0.0e+00
Identity = 634/764 (82.98%), Postives = 684/764 (89.53%), Query Frame = 0

Query: 16  MGFREVWVLLSIMLATSTAAVDQQTYIIHMDTTKMATTNPEQWYMDMIDSVNKISSL-DE 75
           MGFREV + LSI LATSTAAVDQQ+YIIHMDTTKMA   PEQWY  +IDS+N+ISSL D+
Sbjct: 1   MGFREVCLFLSIFLATSTAAVDQQSYIIHMDTTKMAAPTPEQWYTALIDSINEISSLHDQ 60

Query: 76  EEASNAAEILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGL 135
           +EASNAA+ILYVYKTAISGFAAKLST+KLHSLSK PGFLAATP+ELLQLHTTHSPQFLGL
Sbjct: 61  QEASNAAQILYVYKTAISGFAAKLSTKKLHSLSKTPGFLAATPNELLQLHTTHSPQFLGL 120

Query: 136 QRGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPKKWKGICQAGPKFSRSNCN 195
           QR HGLWNSSNLASDI+IG+LDTGIWPEHISFQDKGLPPVPKKWKG CQ G KFS SNCN
Sbjct: 121 QRQHGLWNSSNLASDIVIGLLDTGIWPEHISFQDKGLPPVPKKWKGTCQPGQKFSPSNCN 180

Query: 196 KKLVGARAYIKGYETIVGKLNATGTFRSPRDSDGHGTHTASTAAGNIVYRASLYNQGMGV 255
           +KL+GA AYIKGYE IVG+LNATGTFRSPRDSDGHGTHTASTAAGNIV +AS +NQ MGV
Sbjct: 181 RKLIGAMAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVNKASFFNQAMGV 240

Query: 256 ATGMGFNSRIAAYKVCWQEGCANTDILAAIDRAVADGVDVLSLSLGGGAGFFDRDGIAIA 315
           ATGM F SRIAAYKVCW EGCAN DILAAIDRAVADGVDVLSLSLGG A  F +D IAIA
Sbjct: 241 ATGMRFTSRIAAYKVCWTEGCANADILAAIDRAVADGVDVLSLSLGGSASAFYKDDIAIA 300

Query: 316 AFGAVRNGVFVSCSAGNSGPYISTVSNVAPWIMTVAASYTDRTFPAIVKLGNGKVFEGSS 375
            FGAVRNGVFVSCSAGNSGP  STVSN+APWIMTVAASYTDRTFPA VKLGNG+VFEGSS
Sbjct: 301 TFGAVRNGVFVSCSAGNSGPSSSTVSNIAPWIMTVAASYTDRTFPATVKLGNGQVFEGSS 360

Query: 376 LYSGKNINQLPLVYNNTAGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTEKGEQVKLA 435
           LY+G NI QLPLVYNNTAGGQEANVCT GSLVPS VKGKIVVCERGTNSRT KGEQVKLA
Sbjct: 361 LYTGNNIGQLPLVYNNTAGGQEANVCTPGSLVPSMVKGKIVVCERGTNSRTAKGEQVKLA 420

Query: 436 GGAGMIVINTELEGEELLADLHVLPATALGFSAGKAIINYIASSKHQPRASITFEGTKFG 495
           GGAGMI+INT+LEGEEL AD HVLPA  LG SAG+AII YI+SSKHQP+A I FEGTKFG
Sbjct: 421 GGAGMILINTQLEGEELFADPHVLPAANLGASAGQAIIRYISSSKHQPKALIGFEGTKFG 480

Query: 496 SQAPRVAAFSSRGPTLFKPYVIKPDITAPGVNILAAWPPITSPTELESDKRRVLFNIISG 555
           ++APRVAAFSSRGP+L  P VIKPD+TAPGVNILAAWP I SP+ELESDKRRVLFN+ISG
Sbjct: 481 NRAPRVAAFSSRGPSLIAPDVIKPDVTAPGVNILAAWPLIASPSELESDKRRVLFNVISG 540

Query: 556 TSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYITDNKMSLIPDAGRASGRPADPFA 615
           TSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAY  DN+MS I D G ASG+PA+PFA
Sbjct: 541 TSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNRMSPISDVGSASGKPANPFA 600

Query: 616 YGSGHVDPEKASDPGLIYDIAPQDYLKYLCSLNYTSSQVALVSRGNFACPSKRKLLKPGD 675
           +GSGHVDPEKASDPGLIYDI PQDYL Y CSLNY S+Q+ LVSRGNF CPSKR++ +PG+
Sbjct: 601 FGSGHVDPEKASDPGLIYDITPQDYLDYFCSLNYNSTQIGLVSRGNFTCPSKRRVGQPGN 660

Query: 676 LNYPSFSVLMKKKAKNVIVTFKRTVTNVGSPSSDYTIIVNNPKGVRVIVKPEMLSFRRLG 735
           LNYPSFSV MKKKAKNV VT KRTVTNVG P SDY++ + NPKG+ + VKPE LSFRR G
Sbjct: 661 LNYPSFSVFMKKKAKNVSVTLKRTVTNVGGPRSDYSVKIKNPKGIVISVKPEKLSFRRYG 720

Query: 736 QKLSYQVSFVALGKGEALSDFSFGSLIWFSGKYVVRSPIAVTWQ 779
           QKLSYQVSFVALGK E +S FSFGSL+W SGKY VRSPIAVTW+
Sbjct: 721 QKLSYQVSFVALGKREGVSGFSFGSLVWVSGKYAVRSPIAVTWK 764

BLAST of Lag0041261 vs. ExPASy Swiss-Prot
Match: Q84WS0 (Subtilisin-like protease SBT1.1 OS=Arabidopsis thaliana OX=3702 GN=SBTI1.1 PE=1 SV=1)

HSP 1 Score: 819.7 bits (2116), Expect = 2.9e-236
Identity = 423/765 (55.29%), Postives = 548/765 (71.63%), Query Frame = 0

Query: 16  MGFREVWVLLSIMLATSTAAVDQQTYIIHMDTTKMATTNPEQWYMDMIDSVNKISSLDEE 75
           M FR   V   ++   S  +  +QTY+IH       TT+ +     + +S+   +  D++
Sbjct: 19  MFFRSFIVFFFLIFFASNVSSRKQTYVIH-----TVTTSTKHIVTSLFNSLQTENINDDD 78

Query: 76  EASNAAEILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGLQ 135
              +  EI Y+Y+ A+SGF+A L+  +L ++    GF++A PDELL LHTT+S +FLGL+
Sbjct: 79  --FSLPEIHYIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYSHEFLGLE 138

Query: 136 RGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPKKWKGICQAGPKFSRSNCNK 195
            G GLWN ++L+SD+IIG++DTGI PEH+SF+D  + PVP +W+G C  G  FS S CNK
Sbjct: 139 FGIGLWNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNFSSSECNK 198

Query: 196 KLVGARAYIKGYETIVGKLNATGTFRSPRDSDGHGTHTASTAAGNIVYRASLYNQGMGVA 255
           K++GA A+ KGYE+IVGK+N T  FRS RD+ GHGTHTASTAAG+IV +A+ + Q  G+A
Sbjct: 199 KIIGASAFYKGYESIVGKINETTDFRSTRDAQGHGTHTASTAAGDIVPKANYFGQAKGLA 258

Query: 256 TGMGFNSRIAAYKVCWQEGCANTDILAAIDRAVADGVDVLSLSLGGGAGFFDRDGIAIAA 315
           +GM F SRIAAYK CW  GCA+TD++AAIDRA+ DGVDV+SLSLGG +  F  D IAIA 
Sbjct: 259 SGMRFTSRIAAYKACWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFYVDPIAIAG 318

Query: 316 FGAVRNGVFVSCSAGNSGPYISTVSNVAPWIMTVAASYTDRTFPAIVKLGNGKVFEGSSL 375
           FGA++  +FVSCSAGNSGP  STVSN APW+MTVAASYTDRTFPAIV++GN K   GSSL
Sbjct: 319 FGAMQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNRKSLVGSSL 378

Query: 376 YSGKNINQLPLVYNNTAGGQEANV-CTAGSLVPSKVKGKIVVCERGTNSRTEKGEQVKLA 435
           Y GK++  LPL +N TAG +   V C   SL    V+GKIV+C RG + RT KGE+VK +
Sbjct: 379 YKGKSLKNLPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGASGRTAKGEEVKRS 438

Query: 436 GGAGMIVINTELEGEELLADLHVLPATALGFSAGKAIINYIASSKHQPRASITFEGTKFG 495
           GGA M++++TE EGEELLAD HVLPA +LGFS GK ++NY+A + +   AS+ F GT +G
Sbjct: 439 GGAAMLLVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLAGAAN-ATASVRFRGTAYG 498

Query: 496 SQAPRVAAFSSRGPTLFKPYVIKPDITAPGVNILAAWPPITSPTELESDKRRVLFNIISG 555
           + AP VAAFSSRGP++  P + KPDI APG+NILA W P +SP+ L SD RRV FNIISG
Sbjct: 499 ATAPMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRSDPRRVQFNIISG 558

Query: 556 TSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYITDNKMSLIPDAGRASGR-PADPF 615
           TSM+CPH+SG+AAL+KS H DWSPA IKSA+MTTA ITDN+   I D G A     A  F
Sbjct: 559 TSMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRGAAGAESAATAF 618

Query: 616 AYGSGHVDPEKASDPGLIYDIAPQDYLKYLCSLNYTSSQVALVSRGNFACPSKRKLLKPG 675
           A+G+G+VDP +A DPGL+YD +  DYL YLCSLNYTS ++ L S  N+ C S   +L PG
Sbjct: 619 AFGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYTCASNAVVLSPG 678

Query: 676 DLNYPSFSVLMKKKAKNVIVTFKRTVTNVGSPSSDYTIIVNNPKGVRVIVKPEMLSFRRL 735
           DLNYPSF+V +   A    V +KRTVTNVGSP+ +Y + V  PKGV+V V+P++L F++ 
Sbjct: 679 DLNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKVRVEPKVLKFQKA 738

Query: 736 GQKLSYQVSFVALGKGEALSDFSFGSLIWFSGKYVVRSPIAVTWQ 779
            ++LSY V++ A     + S  SFG L+W   KY VRSPIAVTW+
Sbjct: 739 RERLSYTVTYDAEASRNS-SSSSFGVLVWICDKYNVRSPIAVTWE 774

BLAST of Lag0041261 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 699.1 bits (1803), Expect = 5.6e-200
Identity = 374/756 (49.47%), Postives = 500/756 (66.14%), Query Frame = 0

Query: 32  STAAVDQQTYIIHMDTTKMATTNP--EQWYMDMIDSVNKISSLDEEEASNAAEILYVYKT 91
           S+++ DQ TYI+HM  ++M ++      WY   + S+           S++AE+LY Y+ 
Sbjct: 23  SSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSI-----------SDSAELLYTYEN 82

Query: 92  AISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGL-QRGHGLWNSSNLAS 151
           AI GF+ +L+  +  SL   PG ++  P+   +LHTT +P FLGL +    L+  +   S
Sbjct: 83  AIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYS 142

Query: 152 DIIIGVLDTGIWPEHISFQDKGLPPVPKKWKGICQAGPKFSRSNCNKKLVGARAYIKGYE 211
           D+++GVLDTG+WPE  S+ D+G  P+P  WKG C+AG  F+ S CN+KL+GAR + +GYE
Sbjct: 143 DVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYE 202

Query: 212 TIVGKLNATGTFRSPRDSDGHGTHTASTAAGNIVYRASLYNQGMGVATGMGFNSRIAAYK 271
           + +G ++ +   RSPRD DGHGTHT+STAAG++V  ASL     G A GM   +R+A YK
Sbjct: 203 STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYK 262

Query: 272 VCWQEGCANTDILAAIDRAVADGVDVLSLSLGGGAGFFDRDGIAIAAFGAVRNGVFVSCS 331
           VCW  GC ++DILAAID+A+AD V+VLS+SLGGG   + RDG+AI AF A+  G+ VSCS
Sbjct: 263 VCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCS 322

Query: 332 AGNSGPYISTVSNVAPWIMTVAASYTDRTFPAIVKLGNGKVFEGSSLYSGKNI--NQLPL 391
           AGN+GP  S++SNVAPWI TV A   DR FPA+  LGNGK F G SL+ G+ +    LP 
Sbjct: 323 AGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPF 382

Query: 392 VY-NNTAGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTEKGEQVKLAGGAGMIVINTE 451
           +Y  N +     N+C  G+L+P KVKGKIV+C+RG N+R +KG+ VK AGG GMI+ NT 
Sbjct: 383 IYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTA 442

Query: 452 LEGEELLADLHVLPATALGFSAGKAIINYIASSKHQPRASITFEGTKFG-SQAPRVAAFS 511
             GEEL+AD H+LPAT +G  AG  I +Y+ +  + P ASI+  GT  G   +P VAAFS
Sbjct: 443 ANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPN-PTASISILGTVVGVKPSPVVAAFS 502

Query: 512 SRGPTLFKPYVIKPDITAPGVNILAAWPPITSPTELESDKRRVLFNIISGTSMSCPHVSG 571
           SRGP    P ++KPD+ APGVNILAAW     PT L SD RRV FNIISGTSMSCPHVSG
Sbjct: 503 SRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSG 562

Query: 572 LAALLKSAHKDWSPAAIKSALMTTAYIT--DNKMSLIPDAGRASGRPADPFAYGSGHVDP 631
           LAALLKS H +WSPAAI+SALMTTAY T  D K    P    A+G+P+ PF +G+GHV P
Sbjct: 563 LAALLKSVHPEWSPAAIRSALMTTAYKTYKDGK----PLLDIATGKPSTPFDHGAGHVSP 622

Query: 632 EKASDPGLIYDIAPQDYLKYLCSLNYTSSQVALVSRGNFAC-PSKRKLLKPGDLNYPSFS 691
             A++PGLIYD+  +DYL +LC+LNYTS Q+  VSR N+ C PSK       DLNYPSF+
Sbjct: 623 TTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKS--YSVADLNYPSFA 682

Query: 692 VLMKKKAKNVIVTFKRTVTNVGSPSSDYTIIVNNPKGVRVIVKPEMLSFRRLGQKLSYQV 751
           V +          + RTVT+VG   +    + +   GV++ V+P +L+F+   +K SY V
Sbjct: 683 VNVDGVG---AYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTV 742

Query: 752 SFVALGKGEALSDFSFGSLIWFSGKYVVRSPIAVTW 778
           +F  +   +     SFGS+ W  GK+VV SP+A++W
Sbjct: 743 TF-TVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of Lag0041261 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 691.4 bits (1783), Expect = 1.2e-197
Identity = 372/767 (48.50%), Postives = 496/767 (64.67%), Query Frame = 0

Query: 24  LLSIMLATSTAAVDQQTYIIHMDTTKMAT--TNPEQWYMDMIDSVNKISSLDEEEASNAA 83
           L+ +   T+T    ++TY+IHMD + M    TN  QWY   I+SV +  S  +EE  N  
Sbjct: 20  LIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKS--QEEEGNNN 79

Query: 84  EILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGLQR--GHG 143
            ILY Y+TA  G AA+L+  +   L +  G +A  P+   +LHTT SP FLGL+R     
Sbjct: 80  RILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESER 139

Query: 144 LWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPKKWKGICQAGPKFSRSNCNKKLVG 203
           +W       D+++GVLDTGIWPE  SF D G+ PVP  W+G C+ G +F + NCN+K+VG
Sbjct: 140 VWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVG 199

Query: 204 ARAYIKGYETIVGKLNATGTFRSPRDSDGHGTHTASTAAGNIVYRASLYNQGMGVATGMG 263
           AR + +GYE   GK++    ++SPRD DGHGTHTA+T AG+ V  A+L+    G A GM 
Sbjct: 200 ARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMA 259

Query: 264 FNSRIAAYKVCWQEGCANTDILAAIDRAVADGVDVLSLSLGGGAGFFDRDGIAIAAFGAV 323
             +R+AAYKVCW  GC ++DIL+A+D+AVADGV VLS+SLGGG   + RD ++IA FGA+
Sbjct: 260 QKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAM 319

Query: 324 RNGVFVSCSAGNSGPYISTVSNVAPWIMTVAASYTDRTFPAIVKLGNGKVFEGSSLYSGK 383
             GVFVSCSAGN GP   +++NV+PWI TV AS  DR FPA VK+G  + F+G SLY G+
Sbjct: 320 EMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGR 379

Query: 384 NI----NQLPLVY--NNTAGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTEKGEQVKL 443
            +     Q PLVY   N +     + C  G+L    V GKIV+C+RG   R +KG+ VK 
Sbjct: 380 TVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKR 439

Query: 444 AGGAGMIVINTELEGEELLADLHVLPATALGFSAGKAIINYIASSKHQPRASITFEGTKF 503
           AGG GM++ NT   GEEL+AD H+LPA A+G   GK I  Y  +SK +  AS+   GT+ 
Sbjct: 440 AGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSK-KATASLEILGTRI 499

Query: 504 G-SQAPRVAAFSSRGPTLFKPYVIKPDITAPGVNILAAWPPITSPTELESDKRRVLFNII 563
           G   +P VAAFSSRGP      ++KPD+ APGVNILAAW    +P+ L SD RRV FNI+
Sbjct: 500 GIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNIL 559

Query: 564 SGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYITDNKMSLIPDAGRASGRPADP 623
           SGTSMSCPHVSG+AAL+KS H DWSPAAIKSALMTTAY+ DN    + DA  A+  P+ P
Sbjct: 560 SGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAA--PSSP 619

Query: 624 FAYGSGHVDPEKASDPGLIYDIAPQDYLKYLCSLNYTSSQVALVSR-GNFACPSKRKLLK 683
           + +G+GH+DP +A+DPGL+YDI PQ+Y ++LC+ + + SQ+ + ++  N  C  K  L K
Sbjct: 620 YDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTC--KHTLAK 679

Query: 684 -PGDLNYPSFSVLMKKKAKNVIVTFKRTVTNVGSPSSDYTIIVNNPKGVRVIVKPEMLSF 743
            PG+LNYP+ S L  +      +T +RTVTNVG   S Y + V+  KG  V V+P+ L+F
Sbjct: 680 NPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNF 739

Query: 744 RRLGQKLSYQVSFVALGKGEALSDFSFGSLIWFSGKYVVRSPIAVTW 778
               QKLSY V+F    +   +    FG L+W S  + VRSP+ +TW
Sbjct: 740 TSKHQKLSYTVTFRTRFR---MKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Lag0041261 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 7.6e-197
Identity = 378/772 (48.96%), Postives = 509/772 (65.93%), Query Frame = 0

Query: 23  VLLSIMLATSTAAVDQQTYIIHMDTTKMAT--TNPEQWYMDMIDSVNKISSLDEEEASNA 82
           +LL     +S+++   ++YI+H+  +   +  ++   W++ ++ S+          +   
Sbjct: 15  LLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSL--------PSSPQP 74

Query: 83  AEILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGLQRGHGL 142
           A +LY Y  A+ GF+A+LS  +  +L + P  ++  PD+  ++HTTH+P FLG  +  GL
Sbjct: 75  ATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGL 134

Query: 143 WNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPKKWKGICQAGPKFSRSNCNKKLVGA 202
           W++SN   D+I+GVLDTGIWPEH SF D GL P+P  WKG C+ GP F  S+CN+KL+GA
Sbjct: 135 WSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGA 194

Query: 203 RAYIKGYETIVG--KLNATGTFRSPRDSDGHGTHTASTAAGNIVYRASLYNQGMGVATGM 262
           RA+ +GY T     K +A    RSPRD++GHGTHTASTAAG++V  ASLY    G ATGM
Sbjct: 195 RAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGM 254

Query: 263 GFNSRIAAYKVCWQEGCANTDILAAIDRAVADGVDVLSLSLG--GGAGFFDRDGIAIAAF 322
              +RIAAYK+CW  GC ++DILAA+D+AVADGV V+SLS+G  G A  +  D IAI AF
Sbjct: 255 ASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAF 314

Query: 323 GAVRNGVFVSCSAGNSGPYISTVSNVAPWIMTVAASYTDRTFPAIVKLGNGKVFEGSSLY 382
           GA R+G+ VSCSAGNSGP   T +N+APWI+TV AS  DR F A    G+GKVF G+SLY
Sbjct: 315 GATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLY 374

Query: 383 SGKNI--NQLPLVYNNTAGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTEKGEQVKLA 442
           +G+++  +QL LVY+   G   + +C  G L  S V+GKIV+C+RG N+R EKG  VKLA
Sbjct: 375 AGESLPDSQLSLVYSGDCG---SRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLA 434

Query: 443 GGAGMIVINTELEGEELLADLHVLPATALGFSAGKAIINYIASSKHQPRASITFEGTKFG 502
           GGAGMI+ NT   GEEL AD H++PAT +G  AG  I +YI +S   P A I+F GT  G
Sbjct: 435 GGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTS-DSPTAKISFLGTLIG 494

Query: 503 SQ--APRVAAFSSRGPTLFKPYVIKPDITAPGVNILAAWPPITSPTELESDKRRVLFNII 562
               +PRVAAFSSRGP    P ++KPD+ APGVNILA W  +  PT+L+ D RRV FNII
Sbjct: 495 PSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNII 554

Query: 563 SGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYITDNKMSLIPDAGRASGRPADP 622
           SGTSMSCPHVSGLAALL+ AH DWSPAAIKSAL+TTAY  +N    I D   A+G+ ++ 
Sbjct: 555 SGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDL--ATGKSSNS 614

Query: 623 FAYGSGHVDPEKASDPGLIYDIAPQDYLKYLCSLNYTSSQVALVSRGNF---ACPSKRKL 682
           F +G+GHVDP KA +PGL+YDI  ++Y+ +LC++ Y    + +  +      AC +  KL
Sbjct: 615 FIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETS-KL 674

Query: 683 LKPGDLNYPSFSVLMKKKAKNVIVTFKRTVTNVGS-PSSDYTIIVNNPKGVRVIVKPEML 742
              GDLNYPSFSV+     +  +V +KR V NVGS   + Y + V +P  V + V P  L
Sbjct: 675 RTAGDLNYPSFSVVFASTGE--VVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKL 734

Query: 743 SFRRLGQKLSYQVSF--VALGKG-EALSDFSFGSLIWFSGKYVVRSPIAVTW 778
           +F +    L Y+V+F  V LG G  ++    FGS+ W  G++VV+SP+AV W
Sbjct: 735 AFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769

BLAST of Lag0041261 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 2.9e-196
Identity = 378/770 (49.09%), Postives = 492/770 (63.90%), Query Frame = 0

Query: 30  ATSTAAVDQQTYIIHMDTTKMATTNPE--QWYMDMIDSVNKISSLDEEEASNAAEILYVY 89
           ++S ++ +  TYI+H+D     +  P    WY   + S+           S+   I++ Y
Sbjct: 17  SSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASL----------TSSPPSIIHTY 76

Query: 90  KTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGLQRGH--GLWNSSN 149
            T   GF+A+L+++    L   P  ++  P+++  LHTT SP+FLGL+     GL   S+
Sbjct: 77  DTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESD 136

Query: 150 LASDIIIGVLDTGIWPEHISFQDKGLPPVPKKWKGICQAGPKFSRSNCNKKLVGARAYIK 209
             SD++IGV+DTG+WPE  SF D+GL PVP KWKG C A   F  S CN+KLVGAR +  
Sbjct: 137 FGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCG 196

Query: 210 GYETIVGKLNATGTFRSPRDSDGHGTHTASTAAGNIVYRASLYNQGMGVATGMGFNSRIA 269
           GYE   GK+N T  FRSPRDSDGHGTHTAS +AG  V+ AS      GVA GM   +R+A
Sbjct: 197 GYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLA 256

Query: 270 AYKVCWQEGCANTDILAAIDRAVADGVDVLSLSLGGGAGFFDRDGIAIAAFGAVRNGVFV 329
           AYKVCW  GC ++DILAA D AVADGVDV+SLS+GG    +  D IAI AFGA+  G+FV
Sbjct: 257 AYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFV 316

Query: 330 SCSAGNSGPYISTVSNVAPWIMTVAASYTDRTFPAIVKLGNGKVFEGSSLYSGKNIN--- 389
           S SAGN GP   TV+NVAPW+ TV A   DR FPA VKLGNGK+  G S+Y G  ++   
Sbjct: 317 SASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGR 376

Query: 390 QLPLVYNNT---AGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTEKGEQVKLAGGAGM 449
             PLVY  +     G  +++C  GSL P+ VKGKIV+C+RG NSR  KGE V+  GG GM
Sbjct: 377 MYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGM 436

Query: 450 IVINTELEGEELLADLHVLPATALGFSAGKAIINYIA------SSKHQPRASITFEGTKF 509
           I+ N   +GE L+AD HVLPAT++G S G  I  YI+      SSKH P A+I F+GT+ 
Sbjct: 437 IIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKH-PTATIVFKGTRL 496

Query: 510 GSQ-APRVAAFSSRGPTLFKPYVIKPDITAPGVNILAAWPPITSPTELESDKRRVLFNII 569
           G + AP VA+FS+RGP    P ++KPD+ APG+NILAAWP    P+ + SD RR  FNI+
Sbjct: 497 GIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNIL 556

Query: 570 SGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYITDNKMSLIPDAGRASGRPADP 629
           SGTSM+CPHVSGLAALLK+AH DWSPAAI+SAL+TTAY  DN  S  P    ++G  +  
Sbjct: 557 SGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDN--SGEPMMDESTGNTSSV 616

Query: 630 FAYGSGHVDPEKASDPGLIYDIAPQDYLKYLCSLNYTSSQVALVSRGNFACPSKRKLLKP 689
             YGSGHV P KA DPGL+YDI   DY+ +LC+ NYT + +  ++R    C   R+    
Sbjct: 617 MDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHV 676

Query: 690 GDLNYPSFSVLMKKKAKNVIVT-FKRTVTNVGSPSSDYTIIVNNPKGVRVIVKPEMLSFR 749
           G+LNYPSFSV+ ++  ++ + T F RTVTNVG   S Y I +  P+G  V V+PE LSFR
Sbjct: 677 GNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFR 736

Query: 750 RLGQKLSY--QVSFVALGKGEALSDFSFGSLIWFSGKYVVRSPIAVTWQE 780
           R+GQKLS+  +V    +      ++   G ++W  GK  V SP+ VT Q+
Sbjct: 737 RVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQQ 773

BLAST of Lag0041261 vs. ExPASy TrEMBL
Match: A0A6J1JN56 (subtilisin-like protease SBT1.1 OS=Cucurbita maxima OX=3661 GN=LOC111486706 PE=3 SV=1)

HSP 1 Score: 1290.4 bits (3338), Expect = 0.0e+00
Identity = 642/766 (83.81%), Postives = 698/766 (91.12%), Query Frame = 0

Query: 16  MGFREVW-VLLSIMLATSTAAVDQQTYIIHMDTTKMATTNPEQWYMDMIDSVNKISSL-- 75
           MG  E+W +LLSIMLATS AAVDQQTYIIHMD TKMATTNPEQWY  +I S+N++SS+  
Sbjct: 1   MGITELWLLLLSIMLATSAAAVDQQTYIIHMDATKMATTNPEQWYTSIIHSINQLSSIND 60

Query: 76  DEEEASNAAEILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFL 135
           D+ EASNAAEILY+YKTAISGF+AKLSTRKLHSLSK+PGFLAATPD+LLQLHTTH+PQFL
Sbjct: 61  DQNEASNAAEILYIYKTAISGFSAKLSTRKLHSLSKLPGFLAATPDKLLQLHTTHTPQFL 120

Query: 136 GLQRGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPKKWKGICQAGPKFSRSN 195
           GLQRGHGLWN+SNLASDIIIGV+DTGIWPEHISFQDKGLPPVPKKWKG CQAGPKFSRSN
Sbjct: 121 GLQRGHGLWNASNLASDIIIGVIDTGIWPEHISFQDKGLPPVPKKWKGTCQAGPKFSRSN 180

Query: 196 CNKKLVGARAYIKGYETIVGKLNATGTFRSPRDSDGHGTHTASTAAGNIVYRASLYNQGM 255
           CNKKLVGARAYIKGYETI+G+LN TGTFRS RDSDGHGTHTASTAAGNIVY+ASLYNQGM
Sbjct: 181 CNKKLVGARAYIKGYETILGRLNTTGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQGM 240

Query: 256 GVATGMGFNSRIAAYKVCWQEGCANTDILAAIDRAVADGVDVLSLSLGGGAGFFDRDGIA 315
           G ATGM F SRIAAYKVCW EGCA+TDILAAIDRAV DGVDVLSLSLGGG GFF +D IA
Sbjct: 241 GAATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVVDGVDVLSLSLGGGDGFFYQDEIA 300

Query: 316 IAAFGAVRNGVFVSCSAGNSGPYISTVSNVAPWIMTVAASYTDRTFPAIVKLGNGKVFEG 375
           IAAFGAVRNGVFVSCSAGNSGP++STV NVAPWIMTVAASYTDRTFP  VKLGNG++FEG
Sbjct: 301 IAAFGAVRNGVFVSCSAGNSGPFMSTVGNVAPWIMTVAASYTDRTFPGSVKLGNGQIFEG 360

Query: 376 SSLYSGKNINQLPLVYNNTAGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTEKGEQVK 435
           SSL+SG +I +LPLVYN TAGG+EA+VCTAGSLVPS VKGKIVVCERGTNSR EKGEQVK
Sbjct: 361 SSLHSGNSIGELPLVYNKTAGGEEASVCTAGSLVPSMVKGKIVVCERGTNSRFEKGEQVK 420

Query: 436 LAGGAGMIVINTELEGEELLADLHVLPATALGFSAGKAIINYIASSKHQPRASITFEGTK 495
           LAGG GMI+INT+LEGEEL  D HVLPA  LG SAGKAIINYIASSKH P+ASI FEGT+
Sbjct: 421 LAGGVGMILINTQLEGEELFGDSHVLPAINLGASAGKAIINYIASSKHPPKASILFEGTR 480

Query: 496 FGSQAPRVAAFSSRGPTLFKPYVIKPDITAPGVNILAAWPPITSPTELESDKRRVLFNII 555
           +GS+APR+AAFSSRGP+ F+PYVIKPDITAPGVNILAAWPP+ SP+EL+SDKRRVLFNII
Sbjct: 481 YGSRAPRMAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPPVVSPSELKSDKRRVLFNII 540

Query: 556 SGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYITDNKMSLIPDAGRASGRPADP 615
           SGTSMSCPHVSG+AALLKSAHK+WSPAAIKSALMTTAY+ DNK SLI D GR SG PADP
Sbjct: 541 SGTSMSCPHVSGIAALLKSAHKNWSPAAIKSALMTTAYVNDNKRSLISDVGRPSGGPADP 600

Query: 616 FAYGSGHVDPEKASDPGLIYDIAPQDYLKYLCSLNYTSSQVALVSRGNFACPSKRKLLKP 675
           +A+GSGHVDPEKA DPGL+YDI PQDYL YLCSLNYTS QV LVSRGNF+CPSKR +L+P
Sbjct: 601 YAFGSGHVDPEKAVDPGLVYDIVPQDYLNYLCSLNYTSKQVGLVSRGNFSCPSKRTVLQP 660

Query: 676 GDLNYPSFSVLMKKKAKNVIVTFKRTVTNVGSPSSDYTIIVNNPKGVRVIVKPEMLSFRR 735
           GDLNYPSFSV MK KAKN  V FKRTVTNVG+PSSDYT+ +NNP G+RV VKPE LSFRR
Sbjct: 661 GDLNYPSFSVSMKNKAKN--VRFKRTVTNVGTPSSDYTVKINNPSGIRVSVKPEKLSFRR 720

Query: 736 LGQKLSYQVSFVALGKGEALSDFSFGSLIWFSGKYVVRSPIAVTWQ 779
            GQKLSYQVSFVALGK E LSDFSFGSL+W SGKY VRSPIAV W+
Sbjct: 721 SGQKLSYQVSFVALGKREGLSDFSFGSLVWVSGKYSVRSPIAVNWE 764

BLAST of Lag0041261 vs. ExPASy TrEMBL
Match: A0A6J1FT86 (subtilisin-like protease SBT1.1 OS=Cucurbita moschata OX=3662 GN=LOC111447921 PE=3 SV=1)

HSP 1 Score: 1269.6 bits (3284), Expect = 0.0e+00
Identity = 629/766 (82.11%), Postives = 693/766 (90.47%), Query Frame = 0

Query: 16  MGFREVWVLLSIMLATS-TAAVDQQTYIIHMDTTKMATTNPEQWYMDMIDSVNKISSL-- 75
           MG  E+W+LLSIMLATS  AA DQQ+YIIHMD TKMATTNP+QWY  +ID++N++SS+  
Sbjct: 1   MGITELWLLLSIMLATSAAAAADQQSYIIHMDATKMATTNPQQWYTSIIDTINQLSSIND 60

Query: 76  DEEEASNAAEILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFL 135
           D+ EASNAAEILY+YKTAISGF+AKLSTRKLHSLSK+PGFL+ATPD+LLQLHTTH+P+FL
Sbjct: 61  DQNEASNAAEILYIYKTAISGFSAKLSTRKLHSLSKLPGFLSATPDKLLQLHTTHTPKFL 120

Query: 136 GLQRGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPKKWKGICQAGPKFSRSN 195
           GLQRGHGLWN+SNLASDIIIGV+DTGIWPEHISFQDKGLP VPKKWKG CQAGPKFSRSN
Sbjct: 121 GLQRGHGLWNASNLASDIIIGVIDTGIWPEHISFQDKGLPAVPKKWKGTCQAGPKFSRSN 180

Query: 196 CNKKLVGARAYIKGYETIVGKLNATGTFRSPRDSDGHGTHTASTAAGNIVYRASLYNQGM 255
           CNKKL+GA AYIKGYETI+G+LN TGTFRS RDSDGHGTHTASTAAGNIVY+ASLYNQGM
Sbjct: 181 CNKKLIGATAYIKGYETILGRLNTTGTFRSARDSDGHGTHTASTAAGNIVYKASLYNQGM 240

Query: 256 GVATGMGFNSRIAAYKVCWQEGCANTDILAAIDRAVADGVDVLSLSLGGGAGFFDRDGIA 315
           G ATGM F SRIAAYKVCW EGCA+TDILAAIDRAV DGVDVLSLSLGGG GFF +D IA
Sbjct: 241 GAATGMRFTSRIAAYKVCWPEGCASTDILAAIDRAVVDGVDVLSLSLGGGDGFFYQDEIA 300

Query: 316 IAAFGAVRNGVFVSCSAGNSGPYISTVSNVAPWIMTVAASYTDRTFPAIVKLGNGKVFEG 375
           IAAFGAVRNGVFVSCSAGNSGP++STV NVAPWIMTVAASYTDRTF   VKLGNG++FEG
Sbjct: 301 IAAFGAVRNGVFVSCSAGNSGPFMSTVGNVAPWIMTVAASYTDRTFAGSVKLGNGQIFEG 360

Query: 376 SSLYSGKNINQLPLVYNNTAGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTEKGEQVK 435
           SSL+SG +I QLPLVYN TAGG+EANVCTAGSLVPS VKGKIVVCERGTNSR EKGEQVK
Sbjct: 361 SSLHSGNSIGQLPLVYNKTAGGEEANVCTAGSLVPSMVKGKIVVCERGTNSRFEKGEQVK 420

Query: 436 LAGGAGMIVINTELEGEELLADLHVLPATALGFSAGKAIINYIASSKHQPRASITFEGTK 495
           LAGG GMI+INT+LEGEEL AD HVLPA  LG SAGKAIINYIASSK  P+ASI FEGT+
Sbjct: 421 LAGGVGMILINTQLEGEELFADSHVLPAVNLGASAGKAIINYIASSKQPPKASILFEGTR 480

Query: 496 FGSQAPRVAAFSSRGPTLFKPYVIKPDITAPGVNILAAWPPITSPTELESDKRRVLFNII 555
           +GS+APR+AAFSSRGP+ F+PYVIKPDITAPGVNILAAWPP+ SP+EL+SDKRRVLFNII
Sbjct: 481 YGSRAPRMAAFSSRGPSFFEPYVIKPDITAPGVNILAAWPPVVSPSELKSDKRRVLFNII 540

Query: 556 SGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYITDNKMSLIPDAGRASGRPADP 615
           SGTSMSCPHVSG+AALLKSAH +WSPAAIKSALMTTAY+ DNK S+I D GR SG PADP
Sbjct: 541 SGTSMSCPHVSGIAALLKSAHNNWSPAAIKSALMTTAYVNDNKRSVISDVGRPSGGPADP 600

Query: 616 FAYGSGHVDPEKASDPGLIYDIAPQDYLKYLCSLNYTSSQVALVSRGNFACPSKRKLLKP 675
           +A+GSGHVDPEKA DPGL+YDI PQDYL YLCSLNYTS QV LVSRGNF+CPS R L++P
Sbjct: 601 YAFGSGHVDPEKAVDPGLVYDIEPQDYLNYLCSLNYTSKQVGLVSRGNFSCPSNRNLVQP 660

Query: 676 GDLNYPSFSVLMKKKAKNVIVTFKRTVTNVGSPSSDYTIIVNNPKGVRVIVKPEMLSFRR 735
           GDLNYPSFSV MK +AKN  VTFKRTVTNVG+P+SDYT+ +NNP G+RV VKP+ LSFRR
Sbjct: 661 GDLNYPSFSVSMKNRAKN--VTFKRTVTNVGTPTSDYTVKINNPSGIRVSVKPKKLSFRR 720

Query: 736 LGQKLSYQVSFVALGKGEALSDFSFGSLIWFSGKYVVRSPIAVTWQ 779
            GQKL YQVSFVALGK E LSDFSFGSL+W SGKY VRSPIAV W+
Sbjct: 721 SGQKLRYQVSFVALGKREGLSDFSFGSLVWVSGKYSVRSPIAVNWE 764

BLAST of Lag0041261 vs. ExPASy TrEMBL
Match: A0A6J1JPC3 (subtilisin-like protease SBT1.1 OS=Cucurbita maxima OX=3661 GN=LOC111487676 PE=3 SV=1)

HSP 1 Score: 1262.3 bits (3265), Expect = 0.0e+00
Identity = 635/764 (83.12%), Postives = 684/764 (89.53%), Query Frame = 0

Query: 16  MGFREVWVLLSIMLATSTAAVDQQTYIIHMDTTKMATTNPEQWYMDMIDSVNKISSL-DE 75
           MGFREV + LSI LATS AAVDQQ+YIIHMDTTKMA  +PEQWY  +IDS+NKISSL D+
Sbjct: 1   MGFREVCLFLSIFLATSAAAVDQQSYIIHMDTTKMAAPSPEQWYTALIDSINKISSLDDQ 60

Query: 76  EEASNAAEILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGL 135
           EEAS+AA+ILYVYKTAISGFAAKLST+KLHSLSK PGFLAATP+ELLQLHTTHSPQFLGL
Sbjct: 61  EEASSAAQILYVYKTAISGFAAKLSTKKLHSLSKTPGFLAATPNELLQLHTTHSPQFLGL 120

Query: 136 QRGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPKKWKGICQAGPKFSRSNCN 195
           QR HGLWNSSNLASDI+IG+LDTGIWPEHISFQDKGLPPVPKKWKG CQAG KFS SNCN
Sbjct: 121 QREHGLWNSSNLASDIVIGLLDTGIWPEHISFQDKGLPPVPKKWKGTCQAGQKFSPSNCN 180

Query: 196 KKLVGARAYIKGYETIVGKLNATGTFRSPRDSDGHGTHTASTAAGNIVYRASLYNQGMGV 255
           +KL+GA AYIKGYE IVG+LNATGTFRSPRDSDGHGTHTASTAAGNIV +AS +NQ MGV
Sbjct: 181 RKLIGATAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVNKASFFNQAMGV 240

Query: 256 ATGMGFNSRIAAYKVCWQEGCANTDILAAIDRAVADGVDVLSLSLGGGAGFFDRDGIAIA 315
           ATGM F SRIAAYKVCW EGCAN DILAAIDRAVADGVDVLSLSLGG A  F +D IAIA
Sbjct: 241 ATGMRFTSRIAAYKVCWTEGCANADILAAIDRAVADGVDVLSLSLGGSASAFYKDDIAIA 300

Query: 316 AFGAVRNGVFVSCSAGNSGPYISTVSNVAPWIMTVAASYTDRTFPAIVKLGNGKVFEGSS 375
            FGAVRNGVFVSCSAGNSGP  STVSN+APWIMTVAASYTDRTFPA VKLGNG+VFEGSS
Sbjct: 301 TFGAVRNGVFVSCSAGNSGPSSSTVSNIAPWIMTVAASYTDRTFPATVKLGNGQVFEGSS 360

Query: 376 LYSGKNINQLPLVYNNTAGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTEKGEQVKLA 435
           LYSG +I QLPLVYNNTAGG+EANVCTAGSLVPS VKGKIVVCERGTNSRT KGEQVKLA
Sbjct: 361 LYSGNSIGQLPLVYNNTAGGEEANVCTAGSLVPSLVKGKIVVCERGTNSRTAKGEQVKLA 420

Query: 436 GGAGMIVINTELEGEELLADLHVLPATALGFSAGKAIINYIASSKHQPRASITFEGTKFG 495
           GGAGMI+INT+LEGEEL AD HVLPA  LG SAG+AII YI+SSKHQP+A I FEGTKFG
Sbjct: 421 GGAGMILINTQLEGEELFADPHVLPAANLGASAGQAIIRYISSSKHQPKALIAFEGTKFG 480

Query: 496 SQAPRVAAFSSRGPTLFKPYVIKPDITAPGVNILAAWPPITSPTELESDKRRVLFNIISG 555
           ++APRVAAFSSRGP+L  P VIKPD+TAPGVNILAAWP I SP+ELESDKRRVLFNIISG
Sbjct: 481 NRAPRVAAFSSRGPSLIAPDVIKPDVTAPGVNILAAWPLIASPSELESDKRRVLFNIISG 540

Query: 556 TSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYITDNKMSLIPDAGRASGRPADPFA 615
           TSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAY  DN+MS I D G  SG+PA+PFA
Sbjct: 541 TSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNRMSPISDVGSESGKPANPFA 600

Query: 616 YGSGHVDPEKASDPGLIYDIAPQDYLKYLCSLNYTSSQVALVSRGNFACPSKRKLLKPGD 675
           +GSGHVDPEKASDPGLIYDI PQDYL Y CSLNY S+Q+ LVSRGNF CPSKR++ +PG+
Sbjct: 601 FGSGHVDPEKASDPGLIYDITPQDYLNYFCSLNYNSTQIGLVSRGNFTCPSKRRVGQPGN 660

Query: 676 LNYPSFSVLMKKKAKNVIVTFKRTVTNVGSPSSDYTIIVNNPKGVRVIVKPEMLSFRRLG 735
           LNYPSFSV MKKKAKNV VT KRTVTNVG P SDYT+ + NPKG+ + V+PE LSFRR G
Sbjct: 661 LNYPSFSVFMKKKAKNVSVTLKRTVTNVGRPRSDYTVKIKNPKGIGIRVEPEKLSFRRYG 720

Query: 736 QKLSYQVSFVALGKGEALSDFSFGSLIWFSGKYVVRSPIAVTWQ 779
           QKLSYQVSFVALGK E L  FSFGSL+W SGKY VRSPIAVTW+
Sbjct: 721 QKLSYQVSFVALGKREGLGGFSFGSLVWVSGKYGVRSPIAVTWK 764

BLAST of Lag0041261 vs. ExPASy TrEMBL
Match: A0A6J1FMR4 (subtilisin-like protease SBT1.1 OS=Cucurbita moschata OX=3662 GN=LOC111446877 PE=3 SV=1)

HSP 1 Score: 1260.7 bits (3261), Expect = 0.0e+00
Identity = 633/764 (82.85%), Postives = 684/764 (89.53%), Query Frame = 0

Query: 16  MGFREVWVLLSIMLATSTAAVDQQTYIIHMDTTKMATTNPEQWYMDMIDSVNKISSL-DE 75
           MGFREV + LSI LATSTAAVDQQ+YIIHMDTTKMA   PEQWY  +IDS+N+ISSL D+
Sbjct: 1   MGFREVCLFLSIFLATSTAAVDQQSYIIHMDTTKMAAPTPEQWYTALIDSINEISSLEDQ 60

Query: 76  EEASNAAEILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGL 135
           EEASNAA+ILYVYKTAISGFAAKLST+KLHSLSK PGFLAATP+ELLQLHTTHSPQFLGL
Sbjct: 61  EEASNAAQILYVYKTAISGFAAKLSTKKLHSLSKTPGFLAATPNELLQLHTTHSPQFLGL 120

Query: 136 QRGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPKKWKGICQAGPKFSRSNCN 195
           QR HGLWNSSNLASDI+IG+LDTGIWPEHISFQDKGLPPVPKKWKG CQAG KFS SNCN
Sbjct: 121 QREHGLWNSSNLASDIVIGLLDTGIWPEHISFQDKGLPPVPKKWKGTCQAGQKFSPSNCN 180

Query: 196 KKLVGARAYIKGYETIVGKLNATGTFRSPRDSDGHGTHTASTAAGNIVYRASLYNQGMGV 255
           +KL+GA AYIKGYE IVG+LNATGTFRSPRDSDGHGTHTASTAAGNIV +AS +NQ MGV
Sbjct: 181 RKLIGAIAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVNKASFFNQAMGV 240

Query: 256 ATGMGFNSRIAAYKVCWQEGCANTDILAAIDRAVADGVDVLSLSLGGGAGFFDRDGIAIA 315
           ATGM F SRIAAYKVCW EGCAN DILAAIDRAVADGVDVLSLSLGG A  F +D IAIA
Sbjct: 241 ATGMRFTSRIAAYKVCWTEGCANADILAAIDRAVADGVDVLSLSLGGSASAFYKDDIAIA 300

Query: 316 AFGAVRNGVFVSCSAGNSGPYISTVSNVAPWIMTVAASYTDRTFPAIVKLGNGKVFEGSS 375
            FGAVRNGVFVSCSAGNSGP  STVSN+APWIMTVAASYTDR+FPA VKLGNG+VFEGSS
Sbjct: 301 TFGAVRNGVFVSCSAGNSGPSSSTVSNIAPWIMTVAASYTDRSFPATVKLGNGQVFEGSS 360

Query: 376 LYSGKNINQLPLVYNNTAGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTEKGEQVKLA 435
           LYSG NI QLPLVYNNTAGG++ANVCTAGSLVPS VKGKIVVCERGTNSRT KGEQVKLA
Sbjct: 361 LYSGNNIGQLPLVYNNTAGGEDANVCTAGSLVPSMVKGKIVVCERGTNSRTAKGEQVKLA 420

Query: 436 GGAGMIVINTELEGEELLADLHVLPATALGFSAGKAIINYIASSKHQPRASITFEGTKFG 495
           GGAGMI+INT+LEGEEL AD HVLPA  LG SAG+AII YI+SSKHQP+A I FEGTKFG
Sbjct: 421 GGAGMILINTQLEGEELFADPHVLPAANLGASAGQAIIRYISSSKHQPKALIAFEGTKFG 480

Query: 496 SQAPRVAAFSSRGPTLFKPYVIKPDITAPGVNILAAWPPITSPTELESDKRRVLFNIISG 555
           ++APRVAAFSSRGP+L  P VIKPD+TAPGVNILAAWP I SP+E+ESDKRRVLFN+ISG
Sbjct: 481 NRAPRVAAFSSRGPSLIAPDVIKPDVTAPGVNILAAWPLIASPSEVESDKRRVLFNVISG 540

Query: 556 TSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYITDNKMSLIPDAGRASGRPADPFA 615
           TSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAY  DN+MS I D G ASG+PA+PFA
Sbjct: 541 TSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNRMSPISDVGSASGKPANPFA 600

Query: 616 YGSGHVDPEKASDPGLIYDIAPQDYLKYLCSLNYTSSQVALVSRGNFACPSKRKLLKPGD 675
           +GSGHVDPEKASDPGLIYDI PQDYL YLCSLNY S+Q+ LVSRGNF CPSKR++ + G 
Sbjct: 601 FGSGHVDPEKASDPGLIYDITPQDYLNYLCSLNYNSTQIGLVSRGNFTCPSKRRVGQAGK 660

Query: 676 LNYPSFSVLMKKKAKNVIVTFKRTVTNVGSPSSDYTIIVNNPKGVRVIVKPEMLSFRRLG 735
           LNYPSFSV MKKKAKNV VT KRTVTNVG P SDY++ + NPKG+ + VKPE LSFRR G
Sbjct: 661 LNYPSFSVFMKKKAKNVSVTLKRTVTNVGRPRSDYSVKIKNPKGIGISVKPEKLSFRRYG 720

Query: 736 QKLSYQVSFVALGKGEALSDFSFGSLIWFSGKYVVRSPIAVTWQ 779
           QKLSYQVSFVALGK E +S FSFGSL+W SG Y VRSPIAVTW+
Sbjct: 721 QKLSYQVSFVALGKREGVSGFSFGSLVWVSGTYAVRSPIAVTWK 764

BLAST of Lag0041261 vs. ExPASy TrEMBL
Match: A0A1S3C0J8 (subtilisin-like protease SBT1.1 OS=Cucumis melo OX=3656 GN=LOC103495084 PE=3 SV=1)

HSP 1 Score: 1226.8 bits (3173), Expect = 0.0e+00
Identity = 620/765 (81.05%), Postives = 672/765 (87.84%), Query Frame = 0

Query: 16  MGFREVWVLLSIMLATSTAAVDQQTYIIHMDTTKMATTNPEQWYMDMIDSVNKISSLDE- 75
           MGFREVWV LSIMLA S+A VDQQTYIIHMDTTKM T NPEQWY D+IDSVN++SSLD+ 
Sbjct: 1   MGFREVWV-LSIMLAISSAVVDQQTYIIHMDTTKMVTPNPEQWYTDIIDSVNELSSLDDN 60

Query: 76  EEASNAAEILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGL 135
           EEASNAAEILYVYKTA+SGFAAKL+++KLHSLSKIPGFLAATP+ELLQLHTTHSPQFLGL
Sbjct: 61  EEASNAAEILYVYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLGL 120

Query: 136 QRGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPKKWKGICQAGPKFSRSNCN 195
           QR HGLWN SNLASDIIIG+LDTGIWPEHISFQDKGL  VP KWKGICQ GP+FS SNCN
Sbjct: 121 QRDHGLWNFSNLASDIIIGLLDTGIWPEHISFQDKGLSSVPLKWKGICQTGPRFSSSNCN 180

Query: 196 KKLVGARAYIKGYETIVGKLNATGTFRSPRDSDGHGTHTASTAAGNIVYRASLYNQGMGV 255
           KKL+GA AYIKGYE IVG+LN TGTFRSPRDSDGHGTHTASTAAG+IV  AS YNQGMGV
Sbjct: 181 KKLIGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGSIVDNASFYNQGMGV 240

Query: 256 ATGMGFNSRIAAYKVCWQEGCANTDILAAIDRAVADGVDVLSLSLGGGAGFFDRDGIAIA 315
           A+GM F SRI AYKVCW  GCAN DILAA+D AVADGVDVLSLSLGGG+  F +D IAIA
Sbjct: 241 ASGMRFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIAIA 300

Query: 316 AFGAVRNGVFVSCSAGNSGPYISTVSNVAPWIMTVAASYTDRTFPAIVKLGNGKVFEGSS 375
           AFGA++ GVFVSCSAGNSGP  STV N APWIMTVAASYTDRTFP  VKLGNG+VFEGSS
Sbjct: 301 AFGAIQKGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGSS 360

Query: 376 LYSGKNINQLPLVYNNTAG-GQEANVCTAGSLVPSKVKGKIVVCERGTNSRTEKGEQVKL 435
           LY GK+IN+LPLVYNNTAG GQE NVC AGSL PS VKGKIV+CERGT SRTEKGEQVKL
Sbjct: 361 LYYGKSINELPLVYNNTAGDGQETNVCIAGSLDPSMVKGKIVICERGTISRTEKGEQVKL 420

Query: 436 AGGAGMIVINTELEGEELLADLHVLPATALGFSAGKAIINYIASSKHQPRASITFEGTKF 495
           AGG GMI+INT+ EGEEL AD HVLPAT LG SAGKAI++YIASSK Q +ASI FEGTK+
Sbjct: 421 AGGTGMILINTQFEGEELFADPHVLPATTLGASAGKAILDYIASSKTQAKASIVFEGTKY 480

Query: 496 GSQAPRVAAFSSRGPTLFKPYVIKPDITAPGVNILAAWPPITSPTELESDKRRVLFNIIS 555
           GSQAPRVAAFSSRGP+L  P VIKPD+TAPGVNILAAWPPI SP+EL SD RRV+FNIIS
Sbjct: 481 GSQAPRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELASDTRRVMFNIIS 540

Query: 556 GTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYITDNKMSLIPDAGRASGRPADPF 615
           GTSMSCPHVSGLAALLKSAH DWSPAAIKSALMTTAY+TD+KMSLI D G+A+G PA PF
Sbjct: 541 GTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYVTDSKMSLISDVGQANGEPATPF 600

Query: 616 AYGSGHVDPEKASDPGLIYDIAPQDYLKYLCSLNYTSSQVALVSRGNFACPSKRKLLKPG 675
            +GSGHVDPEKASDPGLIYDI PQDY+ YLCSL Y SSQ+ALVSRGN  C SKR ++KPG
Sbjct: 601 TFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNSSQIALVSRGNLTCSSKRTVVKPG 660

Query: 676 DLNYPSFSVLMKKKAKNVIVTFKRTVTNVGSPSSDYTIIVNNPKGVRVIVKPEMLSFRRL 735
           DLNYPSFSV MKKKAK V +T KRTVTNVG   SDYT+ +NNPKGV VIVKPE LSF  L
Sbjct: 661 DLNYPSFSVFMKKKAKKVSITLKRTVTNVGITRSDYTVKINNPKGVTVIVKPEKLSFGSL 720

Query: 736 GQKLSYQVSFVALGKGEALSDFSFGSLIWFSGKYVVRSPIAVTWQ 779
           G++LSY+VSFV+LG  EAL  FSFGSL+W SGKY VRSPI VTWQ
Sbjct: 721 GEQLSYKVSFVSLGGKEALDKFSFGSLVWISGKYAVRSPIVVTWQ 764

BLAST of Lag0041261 vs. TAIR 10
Match: AT1G01900.1 (subtilase family protein )

HSP 1 Score: 819.7 bits (2116), Expect = 2.0e-237
Identity = 423/765 (55.29%), Postives = 548/765 (71.63%), Query Frame = 0

Query: 16  MGFREVWVLLSIMLATSTAAVDQQTYIIHMDTTKMATTNPEQWYMDMIDSVNKISSLDEE 75
           M FR   V   ++   S  +  +QTY+IH       TT+ +     + +S+   +  D++
Sbjct: 19  MFFRSFIVFFFLIFFASNVSSRKQTYVIH-----TVTTSTKHIVTSLFNSLQTENINDDD 78

Query: 76  EASNAAEILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGLQ 135
              +  EI Y+Y+ A+SGF+A L+  +L ++    GF++A PDELL LHTT+S +FLGL+
Sbjct: 79  --FSLPEIHYIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYSHEFLGLE 138

Query: 136 RGHGLWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPKKWKGICQAGPKFSRSNCNK 195
            G GLWN ++L+SD+IIG++DTGI PEH+SF+D  + PVP +W+G C  G  FS S CNK
Sbjct: 139 FGIGLWNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNFSSSECNK 198

Query: 196 KLVGARAYIKGYETIVGKLNATGTFRSPRDSDGHGTHTASTAAGNIVYRASLYNQGMGVA 255
           K++GA A+ KGYE+IVGK+N T  FRS RD+ GHGTHTASTAAG+IV +A+ + Q  G+A
Sbjct: 199 KIIGASAFYKGYESIVGKINETTDFRSTRDAQGHGTHTASTAAGDIVPKANYFGQAKGLA 258

Query: 256 TGMGFNSRIAAYKVCWQEGCANTDILAAIDRAVADGVDVLSLSLGGGAGFFDRDGIAIAA 315
           +GM F SRIAAYK CW  GCA+TD++AAIDRA+ DGVDV+SLSLGG +  F  D IAIA 
Sbjct: 259 SGMRFTSRIAAYKACWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFYVDPIAIAG 318

Query: 316 FGAVRNGVFVSCSAGNSGPYISTVSNVAPWIMTVAASYTDRTFPAIVKLGNGKVFEGSSL 375
           FGA++  +FVSCSAGNSGP  STVSN APW+MTVAASYTDRTFPAIV++GN K   GSSL
Sbjct: 319 FGAMQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNRKSLVGSSL 378

Query: 376 YSGKNINQLPLVYNNTAGGQEANV-CTAGSLVPSKVKGKIVVCERGTNSRTEKGEQVKLA 435
           Y GK++  LPL +N TAG +   V C   SL    V+GKIV+C RG + RT KGE+VK +
Sbjct: 379 YKGKSLKNLPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGASGRTAKGEEVKRS 438

Query: 436 GGAGMIVINTELEGEELLADLHVLPATALGFSAGKAIINYIASSKHQPRASITFEGTKFG 495
           GGA M++++TE EGEELLAD HVLPA +LGFS GK ++NY+A + +   AS+ F GT +G
Sbjct: 439 GGAAMLLVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLAGAAN-ATASVRFRGTAYG 498

Query: 496 SQAPRVAAFSSRGPTLFKPYVIKPDITAPGVNILAAWPPITSPTELESDKRRVLFNIISG 555
           + AP VAAFSSRGP++  P + KPDI APG+NILA W P +SP+ L SD RRV FNIISG
Sbjct: 499 ATAPMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRSDPRRVQFNIISG 558

Query: 556 TSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYITDNKMSLIPDAGRASGR-PADPF 615
           TSM+CPH+SG+AAL+KS H DWSPA IKSA+MTTA ITDN+   I D G A     A  F
Sbjct: 559 TSMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRGAAGAESAATAF 618

Query: 616 AYGSGHVDPEKASDPGLIYDIAPQDYLKYLCSLNYTSSQVALVSRGNFACPSKRKLLKPG 675
           A+G+G+VDP +A DPGL+YD +  DYL YLCSLNYTS ++ L S  N+ C S   +L PG
Sbjct: 619 AFGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYTCASNAVVLSPG 678

Query: 676 DLNYPSFSVLMKKKAKNVIVTFKRTVTNVGSPSSDYTIIVNNPKGVRVIVKPEMLSFRRL 735
           DLNYPSF+V +   A    V +KRTVTNVGSP+ +Y + V  PKGV+V V+P++L F++ 
Sbjct: 679 DLNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKVRVEPKVLKFQKA 738

Query: 736 GQKLSYQVSFVALGKGEALSDFSFGSLIWFSGKYVVRSPIAVTWQ 779
            ++LSY V++ A     + S  SFG L+W   KY VRSPIAVTW+
Sbjct: 739 RERLSYTVTYDAEASRNS-SSSSFGVLVWICDKYNVRSPIAVTWE 774

BLAST of Lag0041261 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 699.1 bits (1803), Expect = 4.0e-201
Identity = 374/756 (49.47%), Postives = 500/756 (66.14%), Query Frame = 0

Query: 32  STAAVDQQTYIIHMDTTKMATTNP--EQWYMDMIDSVNKISSLDEEEASNAAEILYVYKT 91
           S+++ DQ TYI+HM  ++M ++      WY   + S+           S++AE+LY Y+ 
Sbjct: 23  SSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSI-----------SDSAELLYTYEN 82

Query: 92  AISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGL-QRGHGLWNSSNLAS 151
           AI GF+ +L+  +  SL   PG ++  P+   +LHTT +P FLGL +    L+  +   S
Sbjct: 83  AIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYS 142

Query: 152 DIIIGVLDTGIWPEHISFQDKGLPPVPKKWKGICQAGPKFSRSNCNKKLVGARAYIKGYE 211
           D+++GVLDTG+WPE  S+ D+G  P+P  WKG C+AG  F+ S CN+KL+GAR + +GYE
Sbjct: 143 DVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYE 202

Query: 212 TIVGKLNATGTFRSPRDSDGHGTHTASTAAGNIVYRASLYNQGMGVATGMGFNSRIAAYK 271
           + +G ++ +   RSPRD DGHGTHT+STAAG++V  ASL     G A GM   +R+A YK
Sbjct: 203 STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYK 262

Query: 272 VCWQEGCANTDILAAIDRAVADGVDVLSLSLGGGAGFFDRDGIAIAAFGAVRNGVFVSCS 331
           VCW  GC ++DILAAID+A+AD V+VLS+SLGGG   + RDG+AI AF A+  G+ VSCS
Sbjct: 263 VCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCS 322

Query: 332 AGNSGPYISTVSNVAPWIMTVAASYTDRTFPAIVKLGNGKVFEGSSLYSGKNI--NQLPL 391
           AGN+GP  S++SNVAPWI TV A   DR FPA+  LGNGK F G SL+ G+ +    LP 
Sbjct: 323 AGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPF 382

Query: 392 VY-NNTAGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTEKGEQVKLAGGAGMIVINTE 451
           +Y  N +     N+C  G+L+P KVKGKIV+C+RG N+R +KG+ VK AGG GMI+ NT 
Sbjct: 383 IYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTA 442

Query: 452 LEGEELLADLHVLPATALGFSAGKAIINYIASSKHQPRASITFEGTKFG-SQAPRVAAFS 511
             GEEL+AD H+LPAT +G  AG  I +Y+ +  + P ASI+  GT  G   +P VAAFS
Sbjct: 443 ANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPN-PTASISILGTVVGVKPSPVVAAFS 502

Query: 512 SRGPTLFKPYVIKPDITAPGVNILAAWPPITSPTELESDKRRVLFNIISGTSMSCPHVSG 571
           SRGP    P ++KPD+ APGVNILAAW     PT L SD RRV FNIISGTSMSCPHVSG
Sbjct: 503 SRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSG 562

Query: 572 LAALLKSAHKDWSPAAIKSALMTTAYIT--DNKMSLIPDAGRASGRPADPFAYGSGHVDP 631
           LAALLKS H +WSPAAI+SALMTTAY T  D K    P    A+G+P+ PF +G+GHV P
Sbjct: 563 LAALLKSVHPEWSPAAIRSALMTTAYKTYKDGK----PLLDIATGKPSTPFDHGAGHVSP 622

Query: 632 EKASDPGLIYDIAPQDYLKYLCSLNYTSSQVALVSRGNFAC-PSKRKLLKPGDLNYPSFS 691
             A++PGLIYD+  +DYL +LC+LNYTS Q+  VSR N+ C PSK       DLNYPSF+
Sbjct: 623 TTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKS--YSVADLNYPSFA 682

Query: 692 VLMKKKAKNVIVTFKRTVTNVGSPSSDYTIIVNNPKGVRVIVKPEMLSFRRLGQKLSYQV 751
           V +          + RTVT+VG   +    + +   GV++ V+P +L+F+   +K SY V
Sbjct: 683 VNVDGVG---AYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTV 742

Query: 752 SFVALGKGEALSDFSFGSLIWFSGKYVVRSPIAVTW 778
           +F  +   +     SFGS+ W  GK+VV SP+A++W
Sbjct: 743 TF-TVDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of Lag0041261 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 691.4 bits (1783), Expect = 8.3e-199
Identity = 372/767 (48.50%), Postives = 496/767 (64.67%), Query Frame = 0

Query: 24  LLSIMLATSTAAVDQQTYIIHMDTTKMAT--TNPEQWYMDMIDSVNKISSLDEEEASNAA 83
           L+ +   T+T    ++TY+IHMD + M    TN  QWY   I+SV +  S  +EE  N  
Sbjct: 20  LIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKS--QEEEGNNN 79

Query: 84  EILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGLQR--GHG 143
            ILY Y+TA  G AA+L+  +   L +  G +A  P+   +LHTT SP FLGL+R     
Sbjct: 80  RILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESER 139

Query: 144 LWNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPKKWKGICQAGPKFSRSNCNKKLVG 203
           +W       D+++GVLDTGIWPE  SF D G+ PVP  W+G C+ G +F + NCN+K+VG
Sbjct: 140 VWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVG 199

Query: 204 ARAYIKGYETIVGKLNATGTFRSPRDSDGHGTHTASTAAGNIVYRASLYNQGMGVATGMG 263
           AR + +GYE   GK++    ++SPRD DGHGTHTA+T AG+ V  A+L+    G A GM 
Sbjct: 200 ARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMA 259

Query: 264 FNSRIAAYKVCWQEGCANTDILAAIDRAVADGVDVLSLSLGGGAGFFDRDGIAIAAFGAV 323
             +R+AAYKVCW  GC ++DIL+A+D+AVADGV VLS+SLGGG   + RD ++IA FGA+
Sbjct: 260 QKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAM 319

Query: 324 RNGVFVSCSAGNSGPYISTVSNVAPWIMTVAASYTDRTFPAIVKLGNGKVFEGSSLYSGK 383
             GVFVSCSAGN GP   +++NV+PWI TV AS  DR FPA VK+G  + F+G SLY G+
Sbjct: 320 EMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGR 379

Query: 384 NI----NQLPLVY--NNTAGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTEKGEQVKL 443
            +     Q PLVY   N +     + C  G+L    V GKIV+C+RG   R +KG+ VK 
Sbjct: 380 TVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKR 439

Query: 444 AGGAGMIVINTELEGEELLADLHVLPATALGFSAGKAIINYIASSKHQPRASITFEGTKF 503
           AGG GM++ NT   GEEL+AD H+LPA A+G   GK I  Y  +SK +  AS+   GT+ 
Sbjct: 440 AGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSK-KATASLEILGTRI 499

Query: 504 G-SQAPRVAAFSSRGPTLFKPYVIKPDITAPGVNILAAWPPITSPTELESDKRRVLFNII 563
           G   +P VAAFSSRGP      ++KPD+ APGVNILAAW    +P+ L SD RRV FNI+
Sbjct: 500 GIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNIL 559

Query: 564 SGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYITDNKMSLIPDAGRASGRPADP 623
           SGTSMSCPHVSG+AAL+KS H DWSPAAIKSALMTTAY+ DN    + DA  A+  P+ P
Sbjct: 560 SGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAA--PSSP 619

Query: 624 FAYGSGHVDPEKASDPGLIYDIAPQDYLKYLCSLNYTSSQVALVSR-GNFACPSKRKLLK 683
           + +G+GH+DP +A+DPGL+YDI PQ+Y ++LC+ + + SQ+ + ++  N  C  K  L K
Sbjct: 620 YDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTC--KHTLAK 679

Query: 684 -PGDLNYPSFSVLMKKKAKNVIVTFKRTVTNVGSPSSDYTIIVNNPKGVRVIVKPEMLSF 743
            PG+LNYP+ S L  +      +T +RTVTNVG   S Y + V+  KG  V V+P+ L+F
Sbjct: 680 NPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNF 739

Query: 744 RRLGQKLSYQVSFVALGKGEALSDFSFGSLIWFSGKYVVRSPIAVTW 778
               QKLSY V+F    +   +    FG L+W S  + VRSP+ +TW
Sbjct: 740 TSKHQKLSYTVTFRTRFR---MKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Lag0041261 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 688.7 bits (1776), Expect = 5.4e-198
Identity = 378/772 (48.96%), Postives = 509/772 (65.93%), Query Frame = 0

Query: 23  VLLSIMLATSTAAVDQQTYIIHMDTTKMAT--TNPEQWYMDMIDSVNKISSLDEEEASNA 82
           +LL     +S+++   ++YI+H+  +   +  ++   W++ ++ S+          +   
Sbjct: 15  LLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSL--------PSSPQP 74

Query: 83  AEILYVYKTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGLQRGHGL 142
           A +LY Y  A+ GF+A+LS  +  +L + P  ++  PD+  ++HTTH+P FLG  +  GL
Sbjct: 75  ATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGL 134

Query: 143 WNSSNLASDIIIGVLDTGIWPEHISFQDKGLPPVPKKWKGICQAGPKFSRSNCNKKLVGA 202
           W++SN   D+I+GVLDTGIWPEH SF D GL P+P  WKG C+ GP F  S+CN+KL+GA
Sbjct: 135 WSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGA 194

Query: 203 RAYIKGYETIVG--KLNATGTFRSPRDSDGHGTHTASTAAGNIVYRASLYNQGMGVATGM 262
           RA+ +GY T     K +A    RSPRD++GHGTHTASTAAG++V  ASLY    G ATGM
Sbjct: 195 RAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGM 254

Query: 263 GFNSRIAAYKVCWQEGCANTDILAAIDRAVADGVDVLSLSLG--GGAGFFDRDGIAIAAF 322
              +RIAAYK+CW  GC ++DILAA+D+AVADGV V+SLS+G  G A  +  D IAI AF
Sbjct: 255 ASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAF 314

Query: 323 GAVRNGVFVSCSAGNSGPYISTVSNVAPWIMTVAASYTDRTFPAIVKLGNGKVFEGSSLY 382
           GA R+G+ VSCSAGNSGP   T +N+APWI+TV AS  DR F A    G+GKVF G+SLY
Sbjct: 315 GATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLY 374

Query: 383 SGKNI--NQLPLVYNNTAGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTEKGEQVKLA 442
           +G+++  +QL LVY+   G   + +C  G L  S V+GKIV+C+RG N+R EKG  VKLA
Sbjct: 375 AGESLPDSQLSLVYSGDCG---SRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLA 434

Query: 443 GGAGMIVINTELEGEELLADLHVLPATALGFSAGKAIINYIASSKHQPRASITFEGTKFG 502
           GGAGMI+ NT   GEEL AD H++PAT +G  AG  I +YI +S   P A I+F GT  G
Sbjct: 435 GGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTS-DSPTAKISFLGTLIG 494

Query: 503 SQ--APRVAAFSSRGPTLFKPYVIKPDITAPGVNILAAWPPITSPTELESDKRRVLFNII 562
               +PRVAAFSSRGP    P ++KPD+ APGVNILA W  +  PT+L+ D RRV FNII
Sbjct: 495 PSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNII 554

Query: 563 SGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYITDNKMSLIPDAGRASGRPADP 622
           SGTSMSCPHVSGLAALL+ AH DWSPAAIKSAL+TTAY  +N    I D   A+G+ ++ 
Sbjct: 555 SGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDL--ATGKSSNS 614

Query: 623 FAYGSGHVDPEKASDPGLIYDIAPQDYLKYLCSLNYTSSQVALVSRGNF---ACPSKRKL 682
           F +G+GHVDP KA +PGL+YDI  ++Y+ +LC++ Y    + +  +      AC +  KL
Sbjct: 615 FIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETS-KL 674

Query: 683 LKPGDLNYPSFSVLMKKKAKNVIVTFKRTVTNVGS-PSSDYTIIVNNPKGVRVIVKPEML 742
              GDLNYPSFSV+     +  +V +KR V NVGS   + Y + V +P  V + V P  L
Sbjct: 675 RTAGDLNYPSFSVVFASTGE--VVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKL 734

Query: 743 SFRRLGQKLSYQVSF--VALGKG-EALSDFSFGSLIWFSGKYVVRSPIAVTW 778
           +F +    L Y+V+F  V LG G  ++    FGS+ W  G++VV+SP+AV W
Sbjct: 735 AFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769

BLAST of Lag0041261 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 686.8 bits (1771), Expect = 2.0e-197
Identity = 378/770 (49.09%), Postives = 492/770 (63.90%), Query Frame = 0

Query: 30  ATSTAAVDQQTYIIHMDTTKMATTNPE--QWYMDMIDSVNKISSLDEEEASNAAEILYVY 89
           ++S ++ +  TYI+H+D     +  P    WY   + S+           S+   I++ Y
Sbjct: 17  SSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASL----------TSSPPSIIHTY 76

Query: 90  KTAISGFAAKLSTRKLHSLSKIPGFLAATPDELLQLHTTHSPQFLGLQRGH--GLWNSSN 149
            T   GF+A+L+++    L   P  ++  P+++  LHTT SP+FLGL+     GL   S+
Sbjct: 77  DTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESD 136

Query: 150 LASDIIIGVLDTGIWPEHISFQDKGLPPVPKKWKGICQAGPKFSRSNCNKKLVGARAYIK 209
             SD++IGV+DTG+WPE  SF D+GL PVP KWKG C A   F  S CN+KLVGAR +  
Sbjct: 137 FGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCG 196

Query: 210 GYETIVGKLNATGTFRSPRDSDGHGTHTASTAAGNIVYRASLYNQGMGVATGMGFNSRIA 269
           GYE   GK+N T  FRSPRDSDGHGTHTAS +AG  V+ AS      GVA GM   +R+A
Sbjct: 197 GYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLA 256

Query: 270 AYKVCWQEGCANTDILAAIDRAVADGVDVLSLSLGGGAGFFDRDGIAIAAFGAVRNGVFV 329
           AYKVCW  GC ++DILAA D AVADGVDV+SLS+GG    +  D IAI AFGA+  G+FV
Sbjct: 257 AYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFV 316

Query: 330 SCSAGNSGPYISTVSNVAPWIMTVAASYTDRTFPAIVKLGNGKVFEGSSLYSGKNIN--- 389
           S SAGN GP   TV+NVAPW+ TV A   DR FPA VKLGNGK+  G S+Y G  ++   
Sbjct: 317 SASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGR 376

Query: 390 QLPLVYNNT---AGGQEANVCTAGSLVPSKVKGKIVVCERGTNSRTEKGEQVKLAGGAGM 449
             PLVY  +     G  +++C  GSL P+ VKGKIV+C+RG NSR  KGE V+  GG GM
Sbjct: 377 MYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGM 436

Query: 450 IVINTELEGEELLADLHVLPATALGFSAGKAIINYIA------SSKHQPRASITFEGTKF 509
           I+ N   +GE L+AD HVLPAT++G S G  I  YI+      SSKH P A+I F+GT+ 
Sbjct: 437 IIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKH-PTATIVFKGTRL 496

Query: 510 GSQ-APRVAAFSSRGPTLFKPYVIKPDITAPGVNILAAWPPITSPTELESDKRRVLFNII 569
           G + AP VA+FS+RGP    P ++KPD+ APG+NILAAWP    P+ + SD RR  FNI+
Sbjct: 497 GIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNIL 556

Query: 570 SGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYITDNKMSLIPDAGRASGRPADP 629
           SGTSM+CPHVSGLAALLK+AH DWSPAAI+SAL+TTAY  DN  S  P    ++G  +  
Sbjct: 557 SGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDN--SGEPMMDESTGNTSSV 616

Query: 630 FAYGSGHVDPEKASDPGLIYDIAPQDYLKYLCSLNYTSSQVALVSRGNFACPSKRKLLKP 689
             YGSGHV P KA DPGL+YDI   DY+ +LC+ NYT + +  ++R    C   R+    
Sbjct: 617 MDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHV 676

Query: 690 GDLNYPSFSVLMKKKAKNVIVT-FKRTVTNVGSPSSDYTIIVNNPKGVRVIVKPEMLSFR 749
           G+LNYPSFSV+ ++  ++ + T F RTVTNVG   S Y I +  P+G  V V+PE LSFR
Sbjct: 677 GNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFR 736

Query: 750 RLGQKLSY--QVSFVALGKGEALSDFSFGSLIWFSGKYVVRSPIAVTWQE 780
           R+GQKLS+  +V    +      ++   G ++W  GK  V SP+ VT Q+
Sbjct: 737 RVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQQ 773