Lag0041056 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0041056 vs. NCBI nr
Match: KAG7031505.1 (Rhodanese-like domain-containing protein 7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 796.2 bits (2055), Expect = 1.7e-226
Identity = 402/501 (80.24%), Postives = 424/501 (84.63%), Query Frame = 0

Query: 14  MLRYRASPLLSLGMVSPLL-SSSSSSSSLSSNPNLIPFTPSFPILKPSPPLPSLLSSFLQ 73
           MLRYRA PLL+LGMVSPLL SSSSSSSS SSNPNLI F PSFP+LKPSP LPSLLSSF Q
Sbjct: 1   MLRYRAPPLLALGMVSPLLSSSSSSSSSFSSNPNLITFNPSFPVLKPSPTLPSLLSSFHQ 60

Query: 74  KPSRSMTHSTCFNGPSPAVPIPTPCQAAEMESVTSLVVVSFYKFADFPDHAQFRSPLKKL 133
           KP+RSMTHS CF+GPSPAVPIPT C+A E++SV SLVVVSFYKFADFPDHA+FRSPLKKL
Sbjct: 61  KPTRSMTHSKCFSGPSPAVPIPTACEAQEVDSV-SLVVVSFYKFADFPDHAEFRSPLKKL 120

Query: 134 CEELVVDSLRAFLPAGRLQLLKIITTDFNTLIASWKIRKFLIFTLICACSLRNEVSGGII 193
           C+EL                                                  VSGGII
Sbjct: 121 CQEL-------------------------------------------------RVSGGII 180

Query: 194 LAPEGINGSICGTRESVQRVLGFIESDERLKGLRQVETPVSSEEEAIHHGHTSSSPLAAG 253
           LAPEGINGSICG+RESVQRVLGFIESDERLKGLRQVETPVS EEEAIHHGH+S SPLAAG
Sbjct: 181 LAPEGINGSICGSRESVQRVLGFIESDERLKGLRQVETPVSPEEEAIHHGHSSGSPLAAG 240

Query: 254 EDAPFRWDHVRVKLKKEIVTLGMPGISPVERVGKYVKPRDWNVLINDPDTVVIDVRNHYE 313
           EDAPFRWDHVRVKLKKEIVTLGMP +SPVERVG+YVKPRDWN LI+DPDTVVIDVRNHYE
Sbjct: 241 EDAPFRWDHVRVKLKKEIVTLGMPDVSPVERVGQYVKPRDWNALISDPDTVVIDVRNHYE 300

Query: 314 TRIGKFKRAVDPCTTAFRDFPVWVEDEFQLPNSDNKHSNQGVEEEAVNPEQNSLPRVAMY 373
           TRIGKFKRAVDPCT AFRDFP WVED+FQLP SD +HSN+ VE + VNPE+NS PRVAMY
Sbjct: 301 TRIGKFKRAVDPCTPAFRDFPFWVEDKFQLPKSDCEHSNEEVEGKTVNPEENSPPRVAMY 360

Query: 374 CTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGL 433
           CTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGL
Sbjct: 361 CTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGL 420

Query: 434 EQGTFKLCYGCKQPVSDADMESPEWEYGVSCPHCYSTKSEEEKERARARQRQFETWGIIG 493
           EQGTFKLCYGCKQPVSDADMESP+WEYGVSCPHCYSTKSEEEKERARARQRQFETWGIIG
Sbjct: 421 EQGTFKLCYGCKQPVSDADMESPQWEYGVSCPHCYSTKSEEEKERARARQRQFETWGIIG 451

Query: 494 GPDKGRRPKEGDNNLNLPNSV 514
           GPDKGRRPK+ D N  LPNS+
Sbjct: 481 GPDKGRRPKQSDKNATLPNSI 451

BLAST of Lag0041056 vs. NCBI nr
Match: XP_022942932.1 (rhodanese-like domain-containing protein 7 [Cucurbita moschata])

HSP 1 Score: 792.0 bits (2044), Expect = 3.2e-225
Identity = 401/501 (80.04%), Postives = 422/501 (84.23%), Query Frame = 0

Query: 14  MLRYRASPLLSLGMVSPLL-SSSSSSSSLSSNPNLIPFTPSFPILKPSPPLPSLLSSFLQ 73
           MLRYRA PLL+LGMVSPLL SSSSSSSS SSNPNLI F PSF +LKPSP LPSLLSSF Q
Sbjct: 1   MLRYRAPPLLALGMVSPLLTSSSSSSSSFSSNPNLITFNPSFLVLKPSPTLPSLLSSFHQ 60

Query: 74  KPSRSMTHSTCFNGPSPAVPIPTPCQAAEMESVTSLVVVSFYKFADFPDHAQFRSPLKKL 133
           KP+RSMTHS CF+GPSPAVPIPT C+A E++SV SLVVVSFYKFADFPDHA+FRSPLKKL
Sbjct: 61  KPTRSMTHSKCFSGPSPAVPIPTACEAQEVDSV-SLVVVSFYKFADFPDHAEFRSPLKKL 120

Query: 134 CEELVVDSLRAFLPAGRLQLLKIITTDFNTLIASWKIRKFLIFTLICACSLRNEVSGGII 193
           C+EL                                                  VSGGII
Sbjct: 121 CQEL-------------------------------------------------RVSGGII 180

Query: 194 LAPEGINGSICGTRESVQRVLGFIESDERLKGLRQVETPVSSEEEAIHHGHTSSSPLAAG 253
           LAPEGINGSICG+RESVQRVLGFIESDERLKGLRQVETPVS EEEAIHHGH+S SPLAAG
Sbjct: 181 LAPEGINGSICGSRESVQRVLGFIESDERLKGLRQVETPVSPEEEAIHHGHSSGSPLAAG 240

Query: 254 EDAPFRWDHVRVKLKKEIVTLGMPGISPVERVGKYVKPRDWNVLINDPDTVVIDVRNHYE 313
           EDAPFRWDHVRVKLKKEIVTLGMP +SPVER G+YVKPRDWN LI+DPDTVVIDVRNHYE
Sbjct: 241 EDAPFRWDHVRVKLKKEIVTLGMPDVSPVERAGQYVKPRDWNALISDPDTVVIDVRNHYE 300

Query: 314 TRIGKFKRAVDPCTTAFRDFPVWVEDEFQLPNSDNKHSNQGVEEEAVNPEQNSLPRVAMY 373
           TRIGKFKRAVDPCT AFRDFP WVED+FQLP SD +HSN+ VE + VNPEQNS PRVAMY
Sbjct: 301 TRIGKFKRAVDPCTPAFRDFPFWVEDKFQLPKSDCEHSNEEVEGKTVNPEQNSPPRVAMY 360

Query: 374 CTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGL 433
           CTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGL
Sbjct: 361 CTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGL 420

Query: 434 EQGTFKLCYGCKQPVSDADMESPEWEYGVSCPHCYSTKSEEEKERARARQRQFETWGIIG 493
           EQGTFKLCYGCKQPVSDADMESP+WEYGVSCPHCYSTKSEEEKERARARQRQFETWGIIG
Sbjct: 421 EQGTFKLCYGCKQPVSDADMESPQWEYGVSCPHCYSTKSEEEKERARARQRQFETWGIIG 451

Query: 494 GPDKGRRPKEGDNNLNLPNSV 514
           GPDKGRRPK+ D N  LPNS+
Sbjct: 481 GPDKGRRPKQSDKNATLPNSI 451

BLAST of Lag0041056 vs. NCBI nr
Match: KAG6600870.1 (Rhodanese-like domain-containing protein 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 791.6 bits (2043), Expect = 4.1e-225
Identity = 401/502 (79.88%), Postives = 422/502 (84.06%), Query Frame = 0

Query: 14  MLRYRASPLLSLGMVSPLL--SSSSSSSSLSSNPNLIPFTPSFPILKPSPPLPSLLSSFL 73
           MLRYRA PLL+LGMVSPLL  SSSSSSSS SSNPNLI F PSF +LKPSP LPSLLSSF 
Sbjct: 1   MLRYRAPPLLALGMVSPLLTSSSSSSSSSFSSNPNLITFNPSFLVLKPSPTLPSLLSSFH 60

Query: 74  QKPSRSMTHSTCFNGPSPAVPIPTPCQAAEMESVTSLVVVSFYKFADFPDHAQFRSPLKK 133
           QKP+RSMTHS CF+GPSPAVPIPT C+A E++SV SLVVVSFYKFADFPDHA+FRSPLKK
Sbjct: 61  QKPTRSMTHSKCFSGPSPAVPIPTACEAQEVDSV-SLVVVSFYKFADFPDHAEFRSPLKK 120

Query: 134 LCEELVVDSLRAFLPAGRLQLLKIITTDFNTLIASWKIRKFLIFTLICACSLRNEVSGGI 193
           LC+EL                                                  VSGGI
Sbjct: 121 LCQEL-------------------------------------------------RVSGGI 180

Query: 194 ILAPEGINGSICGTRESVQRVLGFIESDERLKGLRQVETPVSSEEEAIHHGHTSSSPLAA 253
           ILAPEGINGSICG+RESVQRVLGFIESDERLKGLRQVETPVS EEEAIHHGH+S SPLAA
Sbjct: 181 ILAPEGINGSICGSRESVQRVLGFIESDERLKGLRQVETPVSPEEEAIHHGHSSGSPLAA 240

Query: 254 GEDAPFRWDHVRVKLKKEIVTLGMPGISPVERVGKYVKPRDWNVLINDPDTVVIDVRNHY 313
           GEDAPFRWDHVRVKLKKEIVTLGMP +SPVER G+YVKPRDWN LI+DPDTVVIDVRNHY
Sbjct: 241 GEDAPFRWDHVRVKLKKEIVTLGMPDVSPVERAGQYVKPRDWNALISDPDTVVIDVRNHY 300

Query: 314 ETRIGKFKRAVDPCTTAFRDFPVWVEDEFQLPNSDNKHSNQGVEEEAVNPEQNSLPRVAM 373
           ETRIGKFKRAVDPCT AFRDFP WVED+FQLP SD +HSN+ VE + VNPEQNS PRVAM
Sbjct: 301 ETRIGKFKRAVDPCTPAFRDFPFWVEDKFQLPKSDCEHSNEEVEGKTVNPEQNSPPRVAM 360

Query: 374 YCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHG 433
           YCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHG
Sbjct: 361 YCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHG 420

Query: 434 LEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPHCYSTKSEEEKERARARQRQFETWGII 493
           LEQGTFKLCYGCKQPVSDADMESP+WEYGVSCPHCYSTKSEEEKERARARQRQFETWGII
Sbjct: 421 LEQGTFKLCYGCKQPVSDADMESPQWEYGVSCPHCYSTKSEEEKERARARQRQFETWGII 452

Query: 494 GGPDKGRRPKEGDNNLNLPNSV 514
           GGPDKGRRPK+ D N  LPNS+
Sbjct: 481 GGPDKGRRPKQSDKNATLPNSI 452

BLAST of Lag0041056 vs. NCBI nr
Match: XP_023513387.1 (rhodanese-like domain-containing protein 7 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 787.7 bits (2033), Expect = 6.0e-224
Identity = 398/501 (79.44%), Postives = 422/501 (84.23%), Query Frame = 0

Query: 14  MLRYRA-SPLLSLGMVSPLLSSSSSSSSLSSNPNLIPFTPSFPILKPSPPLPSLLSSFLQ 73
           MLRYRA  P ++LGMVSPLLSSSSSSSS SSNPNLI F PSFP+LKPSP LPSLLSSF Q
Sbjct: 1   MLRYRAPPPSVALGMVSPLLSSSSSSSSFSSNPNLITFNPSFPVLKPSPTLPSLLSSFHQ 60

Query: 74  KPSRSMTHSTCFNGPSPAVPIPTPCQAAEMESVTSLVVVSFYKFADFPDHAQFRSPLKKL 133
           KP+RSMTHS CF+GPSPAVPIPT C+  E++SV SLVVVSFYKFADFPDHA+FRSPLKKL
Sbjct: 61  KPTRSMTHSKCFSGPSPAVPIPTACEVQEVDSV-SLVVVSFYKFADFPDHAEFRSPLKKL 120

Query: 134 CEELVVDSLRAFLPAGRLQLLKIITTDFNTLIASWKIRKFLIFTLICACSLRNEVSGGII 193
           C+EL                                                  VSGGII
Sbjct: 121 CQEL-------------------------------------------------RVSGGII 180

Query: 194 LAPEGINGSICGTRESVQRVLGFIESDERLKGLRQVETPVSSEEEAIHHGHTSSSPLAAG 253
           LAPEGINGSICG+RESVQRVLGFIESDERLKGLR+VETPVS EEEAIHHGH+SSSPLAAG
Sbjct: 181 LAPEGINGSICGSRESVQRVLGFIESDERLKGLRKVETPVSPEEEAIHHGHSSSSPLAAG 240

Query: 254 EDAPFRWDHVRVKLKKEIVTLGMPGISPVERVGKYVKPRDWNVLINDPDTVVIDVRNHYE 313
           EDAPFRWDHVRVKLKKEIVTLGMP +SPVERVG+YVKPRDWN LI+DPDTVVIDVRNHYE
Sbjct: 241 EDAPFRWDHVRVKLKKEIVTLGMPDVSPVERVGQYVKPRDWNALISDPDTVVIDVRNHYE 300

Query: 314 TRIGKFKRAVDPCTTAFRDFPVWVEDEFQLPNSDNKHSNQGVEEEAVNPEQNSLPRVAMY 373
           TRIGKFKRAVDPCT AFRDFP WVED+FQLP SD +HS + VE + VNPE+NS PRVAMY
Sbjct: 301 TRIGKFKRAVDPCTPAFRDFPFWVEDKFQLPKSDCEHSIEEVEGKTVNPEENSPPRVAMY 360

Query: 374 CTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGL 433
           CTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGL
Sbjct: 361 CTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGL 420

Query: 434 EQGTFKLCYGCKQPVSDADMESPEWEYGVSCPHCYSTKSEEEKERARARQRQFETWGIIG 493
           EQGTFKLCYGCKQPVSDADMESP+WEYGVSCPHCYSTKSEEEKERARARQRQFETWGIIG
Sbjct: 421 EQGTFKLCYGCKQPVSDADMESPQWEYGVSCPHCYSTKSEEEKERARARQRQFETWGIIG 451

Query: 494 GPDKGRRPKEGDNNLNLPNSV 514
           GPDKGRRPK+ D N  LPNS+
Sbjct: 481 GPDKGRRPKQSDKNATLPNSI 451

BLAST of Lag0041056 vs. NCBI nr
Match: XP_022988667.1 (rhodanese-like domain-containing protein 7 [Cucurbita maxima])

HSP 1 Score: 776.2 bits (2003), Expect = 1.8e-220
Identity = 389/487 (79.88%), Postives = 411/487 (84.39%), Query Frame = 0

Query: 27  MVSPLLSSSSSSSSLSSNPNLIPFTPSFPILKPSPPLPSLLSSFLQKPSRSMTHSTCFNG 86
           MVSPLLSSSSS SS SSNPNLI F PSFPILKPSP LPSLLSSF QKPSRSMTHS CF+G
Sbjct: 1   MVSPLLSSSSSYSSFSSNPNLITFNPSFPILKPSPTLPSLLSSFHQKPSRSMTHSKCFSG 60

Query: 87  PSPAVPIPTPCQAAEMESVTSLVVVSFYKFADFPDHAQFRSPLKKLCEELVVDSLRAFLP 146
           PSPAVPIP+ C+  E++S+ SLVVVSFYKFADFPDHA+FRSPLKKLC+EL          
Sbjct: 61  PSPAVPIPSACEVQEVDSM-SLVVVSFYKFADFPDHAEFRSPLKKLCQEL---------- 120

Query: 147 AGRLQLLKIITTDFNTLIASWKIRKFLIFTLICACSLRNEVSGGIILAPEGINGSICGTR 206
                                                   VSGGIILAPEGINGSICG+R
Sbjct: 121 ---------------------------------------RVSGGIILAPEGINGSICGSR 180

Query: 207 ESVQRVLGFIESDERLKGLRQVETPVSSEEEAIHHGHTSSSPLAAGEDAPFRWDHVRVKL 266
           ESVQRVLGFIESDERLKGLRQVETPVS EEEAIHHGH+SSSPLAAGEDAPFRWDHVRVKL
Sbjct: 181 ESVQRVLGFIESDERLKGLRQVETPVSPEEEAIHHGHSSSSPLAAGEDAPFRWDHVRVKL 240

Query: 267 KKEIVTLGMPGISPVERVGKYVKPRDWNVLINDPDTVVIDVRNHYETRIGKFKRAVDPCT 326
           KKEIVTLGMP +SPVERVG+YVKPRDWN LI+DPDTVVIDVRNHYETRIGKFKRAVDPCT
Sbjct: 241 KKEIVTLGMPDVSPVERVGQYVKPRDWNTLISDPDTVVIDVRNHYETRIGKFKRAVDPCT 300

Query: 327 TAFRDFPVWVEDEFQLPNSDNKHSNQGVEEEAVNPEQNSLPRVAMYCTGGIRCEKASSYL 386
            AFRDFP WVED+FQLP SD +HSN+ VE + VNPE+NS PRVAMYCTGGIRCEKASSYL
Sbjct: 301 PAFRDFPFWVEDKFQLPKSDCEHSNEEVEGKTVNPEENSPPRVAMYCTGGIRCEKASSYL 360

Query: 387 LSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGLEQGTFKLCYGCKQP 446
           LSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGLEQGTFKLCYGCKQP
Sbjct: 361 LSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGLEQGTFKLCYGCKQP 420

Query: 447 VSDADMESPEWEYGVSCPHCYSTKSEEEKERARARQRQFETWGIIGGPDKGRRPKEGDNN 506
           VSDAD+ESP+WEYGVSCPHCYSTKSEEEKERARARQRQFETWGIIGGPDKGRRPK+ D N
Sbjct: 421 VSDADIESPQWEYGVSCPHCYSTKSEEEKERARARQRQFETWGIIGGPDKGRRPKQSDKN 437

Query: 507 LNLPNSV 514
             LPNS+
Sbjct: 481 ATLPNSI 437

BLAST of Lag0041056 vs. ExPASy Swiss-Prot
Match: Q1JPN0 (Rhodanese-like domain-containing protein 7 OS=Arabidopsis thaliana OX=3702 GN=STR7 PE=2 SV=1)

HSP 1 Score: 543.9 bits (1400), Expect = 2.0e-153
Identity = 297/488 (60.86%), Postives = 336/488 (68.85%), Query Frame = 0

Query: 29  SPLLSSSSSSSSLSSNPNLIPFTPSFPILKPSPPLPSLLSSFLQKPSRSMTHS-TCFNGP 88
           SP      +S+S+  N        S P L+     P  LSS    PS S+  +  C N  
Sbjct: 29  SPFSGGGVNSNSVGGN--------SKPELQFPQSQPHKLSS---SPSSSLKSTVACSNAG 88

Query: 89  SPAVPIPTPCQA--AEMESVT----SLVVVSFYKFADFPDHAQFRSPLKKLCEELVVDSL 148
           +    + T  QA     ES+     SLVVVSFYKFADFP+HA FR PLK LCE+L     
Sbjct: 89  AIRRSMATVSQAFSERTESIDSDLGSLVVVSFYKFADFPEHADFRKPLKDLCEKL----- 148

Query: 149 RAFLPAGRLQLLKIITTDFNTLIASWKIRKFLIFTLICACSLRNEVSGGIILAPEGINGS 208
                                                        VSGGIILAPEGINGS
Sbjct: 149 --------------------------------------------RVSGGIILAPEGINGS 208

Query: 209 ICGTRESVQRVLGFIESDERLKGLRQVETPVSSEEEAIHHGHTSSSPLAAGEDAPFRWDH 268
           ICG RESV+ VL FI+ D RL GLRQVETPVS E+EAIHHGH+SSSPLAAGEDAPFRWDH
Sbjct: 209 ICGIRESVEEVLAFIQRDVRLNGLRQVETPVSPEQEAIHHGHSSSSPLAAGEDAPFRWDH 268

Query: 269 VRVKLKKEIVTLGMPGISPVERVGKYVKPRDWNVLINDPDTVVIDVRNHYETRIGKFKRA 328
           VRVKLKKEIVTLG+P +SP+ERVG YV P +WN LI+DP+TVVIDVRN YETRIGKFK A
Sbjct: 269 VRVKLKKEIVTLGIPSVSPIERVGTYVSPEEWNELISDPETVVIDVRNTYETRIGKFKGA 328

Query: 329 VDPCTTAFRDFPVWVEDEFQLPNSDNKHSNQGVEEEAVN---------PEQNSLPRVAMY 388
           VDPCTTAFR+FP WVE++F L    N+ +   VE+E  +          +  +LPR+AMY
Sbjct: 329 VDPCTTAFRNFPSWVENQFALKQEGNE-TQAKVEKEDFSEITHKEDKAEKPKTLPRIAMY 388

Query: 389 CTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGL 448
           CTGGIRCEKASS LLS+GF+EVYHL+GGILKYLEEVPKTESLWEGECFVFDKRVSVEHGL
Sbjct: 389 CTGGIRCEKASSLLLSQGFEEVYHLKGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGL 448

Query: 449 EQGTFKLCYGCKQPVSDADMESPEWEYGVSCPHCYSTKSEEEKERARARQRQFETWGIIG 501
            QGT KLCYGCKQP+SD DME+PE+EYGVSCP+CYS KSEEEKERARARQ QFE WG+IG
Sbjct: 449 AQGTHKLCYGCKQPISDEDMEAPEYEYGVSCPYCYSKKSEEEKERARARQTQFEEWGVIG 455

BLAST of Lag0041056 vs. ExPASy Swiss-Prot
Match: Q92LX6 (tRNA uridine(34) hydroxylase OS=Rhizobium meliloti (strain 1021) OX=266834 GN=trhO PE=3 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 2.1e-78
Identity = 157/382 (41.10%), Postives = 209/382 (54.71%), Query Frame = 0

Query: 103 ESVTSLVVVSFYKFADFPDHAQFRSPLKKLCEELVVDSLRAFLPAGRLQLLKIITTDFNT 162
           E+    +V + Y F  FP  A FR PL+ +C+                            
Sbjct: 12  ETPGQFLVAALYHFVSFPRFADFREPLQAICD---------------------------- 71

Query: 163 LIASWKIRKFLIFTLICACSLRNEVSGGIILAPEGINGSICGTRESVQRVLGFIESDERL 222
                                 N V G ++LA EGING+I GT E +  VL  + +    
Sbjct: 72  ---------------------ANGVKGTLLLAHEGINGTIAGTEEGIAAVLACVRAQPEF 131

Query: 223 KGLRQVETPVSSEEEAIHHGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPGISPVE 282
            GL   E+  S+                     PF    ++V+LKKEIVT+G+  I P +
Sbjct: 132 AGLIHKESRASA--------------------MPFL--RMKVRLKKEIVTMGVENIDPKK 191

Query: 283 RVGKYVKPRDWNVLINDPDTVVIDVRNHYETRIGKFKRAVDPCTTAFRDFPVWVEDEFQL 342
            VG YV+P+DWN LI+DP+T+VID RN YET IG F+ AVDP T  FR+FP WV +   L
Sbjct: 192 VVGTYVEPKDWNALISDPETLVIDTRNDYETAIGLFQGAVDPKTKTFREFPEWVRNNSGL 251

Query: 343 PNSDNKHSNQGVEEEAVNPEQNSLPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGIL 402
            N                      P++AMYCTGGIRCEKA++++  +GF+EVYHL+GGIL
Sbjct: 252 HNK---------------------PKIAMYCTGGIRCEKATAFMKEQGFEEVYHLKGGIL 301

Query: 403 KYLEEVPKTESLWEGECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVS 462
           KYLEE+P  ESLWEG CFVFD+RVSV HGL++G   LC+ C+QP++  D++SP  E GVS
Sbjct: 312 KYLEEIPPEESLWEGACFVFDERVSVTHGLQEGEHTLCHACRQPLTPEDLQSPLHEEGVS 301

Query: 463 CPHCYSTKSEEEKERARARQRQ 485
           C HC+ T++EE++ER R RQRQ
Sbjct: 372 CIHCHDTRTEEDRERYRQRQRQ 301

BLAST of Lag0041056 vs. ExPASy Swiss-Prot
Match: Q1GYV1 (tRNA uridine(34) hydroxylase OS=Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) OX=265072 GN=trhO PE=3 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 4.6e-78
Identity = 149/310 (48.06%), Postives = 198/310 (63.87%), Query Frame = 0

Query: 177 LICACSLRNEVSGGIILAPEGINGSICGTRESVQRVLGFIESDERLKGLRQVETPVSSEE 236
           L+  C+ +  + G ++LA EGING++ G+R ++  +L ++  D R+  +   E       
Sbjct: 25  LLALCNAQG-IKGTLLLAEEGINGTVAGSRAAIDALLTYLRQDSRMVDIDHKE------- 84

Query: 237 EAIHHGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPGISPVERVGKYVKPRDWNVL 296
                        +  ++ PF    ++V+LKKEIVTLG+PGISP ++VG YVKP DWN L
Sbjct: 85  -------------SFADEMPFY--RMKVRLKKEIVTLGVPGISPNKKVGTYVKPEDWNTL 144

Query: 297 INDPDTVVIDVRNHYETRIGKFKRAVDPCTTAFRDFPVWVEDEFQLPNSDNKHSNQGVEE 356
           I+DPD VVID RN+YE  IG FK A+DP TT FR FP     EF   N D          
Sbjct: 145 ISDPDVVVIDTRNNYEYEIGTFKGAIDPATTTFRQFP-----EFVRKNLD---------- 204

Query: 357 EAVNPEQNSLPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWE 416
               P +N   +VAM+CTGGIRCEKASSY+L +GFKEVYHL+GGILKYLE +P+ ESLWE
Sbjct: 205 ----PAKNK--KVAMFCTGGIRCEKASSYMLEQGFKEVYHLQGGILKYLETIPEEESLWE 264

Query: 417 GECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPHCYSTKSEEEKE 476
           GECFVFD+RV+V+HGL+ G +  C+ C+ P+S ADM S ++  G+SCP CY   S E++ 
Sbjct: 265 GECFVFDQRVAVKHGLQVGDYDQCFACRHPISAADMASSKYVKGISCPRCYDRMSAEKRA 290

Query: 477 RARARQRQFE 487
           R   RQRQ E
Sbjct: 325 RIAERQRQIE 290

BLAST of Lag0041056 vs. ExPASy Swiss-Prot
Match: Q8U7F8 (tRNA uridine(34) hydroxylase OS=Agrobacterium fabrum (strain C58 / ATCC 33970) OX=176299 GN=trhO PE=3 SV=2)

HSP 1 Score: 292.0 bits (746), Expect = 1.3e-77
Identity = 148/303 (48.84%), Postives = 190/303 (62.71%), Query Frame = 0

Query: 184 RNEVSGGIILAPEGINGSICGTRESVQRVLGFIESDERLKGLRQVETPVSSEEEAIHHGH 243
           +N V G ++LA EGING+I G    VQ VL F+ +      L   E+  S          
Sbjct: 43  KNGVKGTLLLAAEGINGTIAGPDAGVQAVLSFLRAQPEFAALEHKESRAS---------- 102

Query: 244 TSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPGISPVERVGKYVKPRDWNVLINDPDTV 303
                       PF    ++VKLKKEIVT+G+P I P   VG YV PRDWN LI+DPDT+
Sbjct: 103 ----------HMPF--VRLKVKLKKEIVTMGVPDIDPNRIVGTYVDPRDWNALISDPDTI 162

Query: 304 VIDVRNHYETRIGKFKRAVDPCTTAFRDFPVWVEDEFQLPNSDNKHSNQGVEEEAVNPEQ 363
           VID RN YET IG FK AVDP T  FR+FP WV++                     NP  
Sbjct: 163 VIDTRNDYETAIGVFKGAVDPQTKTFREFPDWVKN---------------------NPGL 222

Query: 364 NSLPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFD 423
           ++ P++AMYCTGGIRCEKA++++  +GF EVYHL+GGILKYLEEVP+ ESLWEG CFVFD
Sbjct: 223 HNKPKIAMYCTGGIRCEKATAFMKEQGFDEVYHLKGGILKYLEEVPEEESLWEGACFVFD 282

Query: 424 KRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPHCYSTKSEEEKERARARQR 483
           +RVSV HGL +G  +LC+ C+ P++     SP++E GVSCP CY  +SE++++R R RQ+
Sbjct: 283 ERVSVVHGLAEGDHQLCHACRNPITPDVRLSPKFEEGVSCPSCYDERSEDDRQRFRDRQQ 302

Query: 484 QFE 487
           Q E
Sbjct: 343 QIE 302

BLAST of Lag0041056 vs. ExPASy Swiss-Prot
Match: A6UD82 (tRNA uridine(34) hydroxylase OS=Sinorhizobium medicae (strain WSM419) OX=366394 GN=trhO PE=3 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 6.7e-77
Identity = 155/382 (40.58%), Postives = 208/382 (54.45%), Query Frame = 0

Query: 103 ESVTSLVVVSFYKFADFPDHAQFRSPLKKLCEELVVDSLRAFLPAGRLQLLKIITTDFNT 162
           E+    +V + Y F  FP  A FR PL+ +C+                            
Sbjct: 12  ETPGQFLVAALYHFISFPRFADFREPLQAICD---------------------------- 71

Query: 163 LIASWKIRKFLIFTLICACSLRNEVSGGIILAPEGINGSICGTRESVQRVLGFIESDERL 222
                                 N V G ++LA EGING+I G+ E +  VL F+ +    
Sbjct: 72  ---------------------ANGVKGTLLLAHEGINGTIAGSEEGIATVLAFLRAQPEF 131

Query: 223 KGLRQVETPVSSEEEAIHHGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPGISPVE 282
             L   E+  S+                     PF    ++V+LKKEIVT+G+  I P +
Sbjct: 132 ADLVHKESRASA--------------------MPFL--RMKVRLKKEIVTMGVENIDPKK 191

Query: 283 RVGKYVKPRDWNVLINDPDTVVIDVRNHYETRIGKFKRAVDPCTTAFRDFPVWVEDEFQL 342
            VG YV+P+DWN LI+DP+T+VID RN YET IG F+ AVDP T  FR+FP WV +   L
Sbjct: 192 VVGTYVEPKDWNALISDPETLVIDTRNDYETAIGVFQGAVDPKTKTFREFPEWVRNNSGL 251

Query: 343 PNSDNKHSNQGVEEEAVNPEQNSLPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGIL 402
            N                      P++AMYCTGGIRCEKA++++  +GF+EVYHL+GGIL
Sbjct: 252 HNK---------------------PKIAMYCTGGIRCEKATAFMKEQGFEEVYHLKGGIL 301

Query: 403 KYLEEVPKTESLWEGECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVS 462
           KYLEE+P  ESLWEG CFVFD+RVSV HGL++G   LC+ C+QP++  D+ SP  E GVS
Sbjct: 312 KYLEEIPPEESLWEGACFVFDERVSVTHGLQEGEHTLCHACRQPLTTEDLRSPLHEEGVS 301

Query: 463 CPHCYSTKSEEEKERARARQRQ 485
           C +C+ T++EE++ER R RQRQ
Sbjct: 372 CVNCHDTRTEEDRERYRQRQRQ 301

BLAST of Lag0041056 vs. ExPASy TrEMBL
Match: A0A6J1FRM3 (rhodanese-like domain-containing protein 7 OS=Cucurbita moschata OX=3662 GN=LOC111447816 PE=3 SV=1)

HSP 1 Score: 792.0 bits (2044), Expect = 1.5e-225
Identity = 401/501 (80.04%), Postives = 422/501 (84.23%), Query Frame = 0

Query: 14  MLRYRASPLLSLGMVSPLL-SSSSSSSSLSSNPNLIPFTPSFPILKPSPPLPSLLSSFLQ 73
           MLRYRA PLL+LGMVSPLL SSSSSSSS SSNPNLI F PSF +LKPSP LPSLLSSF Q
Sbjct: 1   MLRYRAPPLLALGMVSPLLTSSSSSSSSFSSNPNLITFNPSFLVLKPSPTLPSLLSSFHQ 60

Query: 74  KPSRSMTHSTCFNGPSPAVPIPTPCQAAEMESVTSLVVVSFYKFADFPDHAQFRSPLKKL 133
           KP+RSMTHS CF+GPSPAVPIPT C+A E++SV SLVVVSFYKFADFPDHA+FRSPLKKL
Sbjct: 61  KPTRSMTHSKCFSGPSPAVPIPTACEAQEVDSV-SLVVVSFYKFADFPDHAEFRSPLKKL 120

Query: 134 CEELVVDSLRAFLPAGRLQLLKIITTDFNTLIASWKIRKFLIFTLICACSLRNEVSGGII 193
           C+EL                                                  VSGGII
Sbjct: 121 CQEL-------------------------------------------------RVSGGII 180

Query: 194 LAPEGINGSICGTRESVQRVLGFIESDERLKGLRQVETPVSSEEEAIHHGHTSSSPLAAG 253
           LAPEGINGSICG+RESVQRVLGFIESDERLKGLRQVETPVS EEEAIHHGH+S SPLAAG
Sbjct: 181 LAPEGINGSICGSRESVQRVLGFIESDERLKGLRQVETPVSPEEEAIHHGHSSGSPLAAG 240

Query: 254 EDAPFRWDHVRVKLKKEIVTLGMPGISPVERVGKYVKPRDWNVLINDPDTVVIDVRNHYE 313
           EDAPFRWDHVRVKLKKEIVTLGMP +SPVER G+YVKPRDWN LI+DPDTVVIDVRNHYE
Sbjct: 241 EDAPFRWDHVRVKLKKEIVTLGMPDVSPVERAGQYVKPRDWNALISDPDTVVIDVRNHYE 300

Query: 314 TRIGKFKRAVDPCTTAFRDFPVWVEDEFQLPNSDNKHSNQGVEEEAVNPEQNSLPRVAMY 373
           TRIGKFKRAVDPCT AFRDFP WVED+FQLP SD +HSN+ VE + VNPEQNS PRVAMY
Sbjct: 301 TRIGKFKRAVDPCTPAFRDFPFWVEDKFQLPKSDCEHSNEEVEGKTVNPEQNSPPRVAMY 360

Query: 374 CTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGL 433
           CTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGL
Sbjct: 361 CTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGL 420

Query: 434 EQGTFKLCYGCKQPVSDADMESPEWEYGVSCPHCYSTKSEEEKERARARQRQFETWGIIG 493
           EQGTFKLCYGCKQPVSDADMESP+WEYGVSCPHCYSTKSEEEKERARARQRQFETWGIIG
Sbjct: 421 EQGTFKLCYGCKQPVSDADMESPQWEYGVSCPHCYSTKSEEEKERARARQRQFETWGIIG 451

Query: 494 GPDKGRRPKEGDNNLNLPNSV 514
           GPDKGRRPK+ D N  LPNS+
Sbjct: 481 GPDKGRRPKQSDKNATLPNSI 451

BLAST of Lag0041056 vs. ExPASy TrEMBL
Match: A0A6J1JDN9 (rhodanese-like domain-containing protein 7 OS=Cucurbita maxima OX=3661 GN=LOC111485926 PE=3 SV=1)

HSP 1 Score: 776.2 bits (2003), Expect = 8.7e-221
Identity = 389/487 (79.88%), Postives = 411/487 (84.39%), Query Frame = 0

Query: 27  MVSPLLSSSSSSSSLSSNPNLIPFTPSFPILKPSPPLPSLLSSFLQKPSRSMTHSTCFNG 86
           MVSPLLSSSSS SS SSNPNLI F PSFPILKPSP LPSLLSSF QKPSRSMTHS CF+G
Sbjct: 1   MVSPLLSSSSSYSSFSSNPNLITFNPSFPILKPSPTLPSLLSSFHQKPSRSMTHSKCFSG 60

Query: 87  PSPAVPIPTPCQAAEMESVTSLVVVSFYKFADFPDHAQFRSPLKKLCEELVVDSLRAFLP 146
           PSPAVPIP+ C+  E++S+ SLVVVSFYKFADFPDHA+FRSPLKKLC+EL          
Sbjct: 61  PSPAVPIPSACEVQEVDSM-SLVVVSFYKFADFPDHAEFRSPLKKLCQEL---------- 120

Query: 147 AGRLQLLKIITTDFNTLIASWKIRKFLIFTLICACSLRNEVSGGIILAPEGINGSICGTR 206
                                                   VSGGIILAPEGINGSICG+R
Sbjct: 121 ---------------------------------------RVSGGIILAPEGINGSICGSR 180

Query: 207 ESVQRVLGFIESDERLKGLRQVETPVSSEEEAIHHGHTSSSPLAAGEDAPFRWDHVRVKL 266
           ESVQRVLGFIESDERLKGLRQVETPVS EEEAIHHGH+SSSPLAAGEDAPFRWDHVRVKL
Sbjct: 181 ESVQRVLGFIESDERLKGLRQVETPVSPEEEAIHHGHSSSSPLAAGEDAPFRWDHVRVKL 240

Query: 267 KKEIVTLGMPGISPVERVGKYVKPRDWNVLINDPDTVVIDVRNHYETRIGKFKRAVDPCT 326
           KKEIVTLGMP +SPVERVG+YVKPRDWN LI+DPDTVVIDVRNHYETRIGKFKRAVDPCT
Sbjct: 241 KKEIVTLGMPDVSPVERVGQYVKPRDWNTLISDPDTVVIDVRNHYETRIGKFKRAVDPCT 300

Query: 327 TAFRDFPVWVEDEFQLPNSDNKHSNQGVEEEAVNPEQNSLPRVAMYCTGGIRCEKASSYL 386
            AFRDFP WVED+FQLP SD +HSN+ VE + VNPE+NS PRVAMYCTGGIRCEKASSYL
Sbjct: 301 PAFRDFPFWVEDKFQLPKSDCEHSNEEVEGKTVNPEENSPPRVAMYCTGGIRCEKASSYL 360

Query: 387 LSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGLEQGTFKLCYGCKQP 446
           LSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGLEQGTFKLCYGCKQP
Sbjct: 361 LSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGLEQGTFKLCYGCKQP 420

Query: 447 VSDADMESPEWEYGVSCPHCYSTKSEEEKERARARQRQFETWGIIGGPDKGRRPKEGDNN 506
           VSDAD+ESP+WEYGVSCPHCYSTKSEEEKERARARQRQFETWGIIGGPDKGRRPK+ D N
Sbjct: 421 VSDADIESPQWEYGVSCPHCYSTKSEEEKERARARQRQFETWGIIGGPDKGRRPKQSDKN 437

Query: 507 LNLPNSV 514
             LPNS+
Sbjct: 481 ATLPNSI 437

BLAST of Lag0041056 vs. ExPASy TrEMBL
Match: A0A6J1CYE5 (rhodanese-like domain-containing protein 7 OS=Momordica charantia OX=3673 GN=LOC111015925 PE=3 SV=1)

HSP 1 Score: 760.4 bits (1962), Expect = 4.9e-216
Identity = 398/518 (76.83%), Postives = 422/518 (81.47%), Query Frame = 0

Query: 3   KKANDSSST-ASMLRYRASPLLS---LGMVSPLLSSSSSSSSLSSNPNLIPFTPSFPILK 62
           K+ NDSSST ASM+RYRA  ++S   L   S   SSSSSSSS SSNPN I F+ SFP+LK
Sbjct: 35  KEPNDSSSTRASMIRYRALAMVSPSPLPSSSSSSSSSSSSSSFSSNPNFITFSSSFPVLK 94

Query: 63  PSPPLPSLLSSF---LQKPSRSMTHSTCFNGPSPAVPIPTPCQAAEMESVTSLVVVSFYK 122
           PS PLPSLLSSF   L+K +RSMT+S CF+G + AVPIP+ C A E +SV SLVVVSFYK
Sbjct: 95  PSLPLPSLLSSFLSALRKHTRSMTYSRCFSGSTTAVPIPSACHAEETDSV-SLVVVSFYK 154

Query: 123 FADFPDHAQFRSPLKKLCEELVVDSLRAFLPAGRLQLLKIITTDFNTLIASWKIRKFLIF 182
           FADFPDHAQFR PLKKLCEEL                                       
Sbjct: 155 FADFPDHAQFRQPLKKLCEEL--------------------------------------- 214

Query: 183 TLICACSLRNEVSGGIILAPEGINGSICGTRESVQRVLGFIESDERLKGLRQVETPVSSE 242
                      VSGGIILAPEGINGSICG+RESVQRVLGFIESDERLKGLRQVETPVS E
Sbjct: 215 ----------RVSGGIILAPEGINGSICGSRESVQRVLGFIESDERLKGLRQVETPVSPE 274

Query: 243 EEAIHHGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPGISPVERVGKYVKPRDWNV 302
           EEAIHHGH SSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMP ISPVERVGKYVKPRDWN 
Sbjct: 275 EEAIHHGHISSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGKYVKPRDWNK 334

Query: 303 LINDPDTVVIDVRNHYETRIGKFKRAVDPCTTAFRDFPVWVEDEFQLPNSDNKHSNQGVE 362
           LI+DPDTVVIDVRNHYETRIGKFK AVDPCTTAFRDFP WVED+FQ+  SD KHSNQGVE
Sbjct: 335 LISDPDTVVIDVRNHYETRIGKFKSAVDPCTTAFRDFPSWVEDKFQVSKSDRKHSNQGVE 394

Query: 363 EEAVNPEQNSLPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLW 422
           E+AVNPEQN+ PRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLW
Sbjct: 395 EDAVNPEQNAPPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLW 454

Query: 423 EGECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPHCYSTKSEEEK 482
           EGECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCP+CYSTKSEEEK
Sbjct: 455 EGECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPYCYSTKSEEEK 502

Query: 483 ERARARQRQFETWGIIGGPDKGRRPKEGDNNLNLPNSV 514
           ERARARQRQFETWGIIGGPDKGR P   +N++NL NS+
Sbjct: 515 ERARARQRQFETWGIIGGPDKGRSPNPSNNSVNLSNSI 502

BLAST of Lag0041056 vs. ExPASy TrEMBL
Match: A0A0A0KM63 (Rhodanese domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G223080 PE=3 SV=1)

HSP 1 Score: 734.9 bits (1896), Expect = 2.2e-208
Identity = 380/506 (75.10%), Postives = 406/506 (80.24%), Query Frame = 0

Query: 14  MLRYRASPLLSLGMVSPLLSSSSSS----SSLSSNPNLIP--FTPSFPILKPSPPLPSLL 73
           ++RYRA PL  LGMVSP  SSSSSS    SS SSN NLI   F+PSFPI +P    PSLL
Sbjct: 2   LIRYRAPPLFPLGMVSPFPSSSSSSFSSLSSFSSNSNLISFNFSPSFPIPRPFLSFPSLL 61

Query: 74  SSFLQKPSRSMTHSTCFNGPSPAVPIPTPCQAAEMESVTSLVVVSFYKFADFPDHAQFRS 133
           S+FL KP RSMTHS CF+GPSP  PI +  + +E     SLVVVSFYKFADFPDHA+FR 
Sbjct: 62  SAFLHKPCRSMTHSVCFSGPSPTAPIHSSSEESEDVESASLVVVSFYKFADFPDHAEFRL 121

Query: 134 PLKKLCEELVVDSLRAFLPAGRLQLLKIITTDFNTLIASWKIRKFLIFTLICACSLRNEV 193
           PLKKLCEEL                                                  V
Sbjct: 122 PLKKLCEEL-------------------------------------------------RV 181

Query: 194 SGGIILAPEGINGSICGTRESVQRVLGFIESDERLKGLRQVETPVSSEEEAIHHGHTSSS 253
           SGGIILAPEGINGSICG+RESVQRVLGFI+SDERLKGLRQVETPVS EEEAIH+GHTSSS
Sbjct: 182 SGGIILAPEGINGSICGSRESVQRVLGFIQSDERLKGLRQVETPVSPEEEAIHNGHTSSS 241

Query: 254 PLAAGEDAPFRWDHVRVKLKKEIVTLGMPGISPVERVGKYVKPRDWNVLINDPDTVVIDV 313
           PLAAGEDAPFRWDHVRVKLKKEIVTLGMP ISPVERVG+YVKPRDWN LI+DPDTVVIDV
Sbjct: 242 PLAAGEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGQYVKPRDWNALISDPDTVVIDV 301

Query: 314 RNHYETRIGKFKRAVDPCTTAFRDFPVWVEDEFQLPNSDNKHSNQGVEEEAVNPEQNSLP 373
           RNHYETRIGKFK AVDPCTTAFRDFP WVED+ QLP  D KHSNQG  EE VNP+++S P
Sbjct: 302 RNHYETRIGKFKTAVDPCTTAFRDFPAWVEDKLQLPKLDCKHSNQG-GEETVNPKESSPP 361

Query: 374 RVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVS 433
           RVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPK+ESLWEGECFVFDKRVS
Sbjct: 362 RVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKSESLWEGECFVFDKRVS 421

Query: 434 VEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPHCYSTKSEEEKERARARQRQFET 493
           VEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCP+C+STKSEEEKERARARQRQFET
Sbjct: 422 VEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPYCFSTKSEEEKERARARQRQFET 457

Query: 494 WGIIGGPDKGRRPKEGDNNLNLPNSV 514
           WGIIGGPDKGRRP + D+N++ PNSV
Sbjct: 482 WGIIGGPDKGRRPTQNDDNVSHPNSV 457

BLAST of Lag0041056 vs. ExPASy TrEMBL
Match: A0A1S4E1C5 (LOW QUALITY PROTEIN: rhodanese-like domain-containing protein 7 OS=Cucumis melo OX=3656 GN=LOC103496761 PE=3 SV=1)

HSP 1 Score: 708.0 bits (1826), Expect = 2.9e-200
Identity = 371/502 (73.90%), Postives = 399/502 (79.48%), Query Frame = 0

Query: 14  MLRYRASPLLSLGMVSPLLSSSSSSSSLSSNPNLIP--FTPSFPILKPSPPLPSLLSSFL 73
           ++RYRA PLL+LGMVSPL   SSSSSS SSN NLI   F+PSFP+ KPS   PSLLSSF+
Sbjct: 2   LIRYRAPPLLALGMVSPL--PSSSSSSFSSNTNLISFNFSPSFPVPKPSSSFPSLLSSFV 61

Query: 74  QKPSRSMTHSTCFNGPSPAVPIPTPCQAAEMESVTSLVVVSFYKFADFPDHAQFRSPLKK 133
            KPSRSMTHS CF+GPSP  PI + C+ +E     SLVVVSFYKFA FPDHA+FRSPLKK
Sbjct: 62  HKPSRSMTHSICFSGPSPTAPIHSSCEESEDMDSISLVVVSFYKFAHFPDHAEFRSPLKK 121

Query: 134 LCEELVVDSLRAFLPAGRLQLLKIITTDFNTLIASWKIRKFLIFTLICACSLRNEVSGGI 193
           LCEEL                                                  V GGI
Sbjct: 122 LCEEL-------------------------------------------------RVLGGI 181

Query: 194 ILAPEGINGSICGTRESVQRVLGFIESDERLKGLRQVETPVSSEEEAIHHGHTSSSPLAA 253
           ILAPEGINGSICG+RESVQRVLGFIESDERLKGLRQVET VS EEEAIH+GHTSSSPLAA
Sbjct: 182 ILAPEGINGSICGSRESVQRVLGFIESDERLKGLRQVETSVSPEEEAIHNGHTSSSPLAA 241

Query: 254 GEDAPFRWDHVRVKLKKEIVTLGMPGISPVERVGKYVKPRDWNVLINDPDTVVIDVRNHY 313
           GEDAPFRWDHVRVKLKKEIVTLGMP ISPVERVG+YVKPRD N LI+DPDTVV DV+NHY
Sbjct: 242 GEDAPFRWDHVRVKLKKEIVTLGMPVISPVERVGQYVKPRDXNSLISDPDTVVTDVQNHY 301

Query: 314 ETRIGKFKRAVDPCTTAFRDFPVWVEDEFQLPNSDNKHSNQGVEEEAVNPEQNSLPRVAM 373
           ETRI KFK AVDP TTAFRDFP WVED+FQLP  D KHSNQG +EE VNPE++S  RVAM
Sbjct: 302 ETRIEKFKTAVDPSTTAFRDFPAWVEDKFQLPKLDCKHSNQG-DEETVNPEESSPLRVAM 361

Query: 374 YCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHG 433
           YCTGGIRCEKASSYLL KGFKEVYHLEGGILKYLEEVP++ESL EGECFVFDKR SVEHG
Sbjct: 362 YCTGGIRCEKASSYLLRKGFKEVYHLEGGILKYLEEVPRSESLREGECFVFDKRDSVEHG 421

Query: 434 LEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPHCYSTKSEEEKERARARQRQFETWGII 493
           LEQGTFKLCYG KQPVSDADMESP+WEYGVSCP+C+S KS+EEKERARARQRQFETWGII
Sbjct: 422 LEQGTFKLCYGYKQPVSDADMESPQWEYGVSCPYCFSMKSDEEKERARARQRQFETWGII 451

Query: 494 GGPDKGRRPKEGDNNLNLPNSV 514
           GGPDKGRRP + DNN+N  NSV
Sbjct: 482 GGPDKGRRPTQNDNNVNHLNSV 451

BLAST of Lag0041056 vs. TAIR 10
Match: AT2G40760.1 (Rhodanese/Cell cycle control phosphatase superfamily protein )

HSP 1 Score: 543.9 bits (1400), Expect = 1.4e-154
Identity = 297/488 (60.86%), Postives = 336/488 (68.85%), Query Frame = 0

Query: 29  SPLLSSSSSSSSLSSNPNLIPFTPSFPILKPSPPLPSLLSSFLQKPSRSMTHS-TCFNGP 88
           SP      +S+S+  N        S P L+     P  LSS    PS S+  +  C N  
Sbjct: 29  SPFSGGGVNSNSVGGN--------SKPELQFPQSQPHKLSS---SPSSSLKSTVACSNAG 88

Query: 89  SPAVPIPTPCQA--AEMESVT----SLVVVSFYKFADFPDHAQFRSPLKKLCEELVVDSL 148
           +    + T  QA     ES+     SLVVVSFYKFADFP+HA FR PLK LCE+L     
Sbjct: 89  AIRRSMATVSQAFSERTESIDSDLGSLVVVSFYKFADFPEHADFRKPLKDLCEKL----- 148

Query: 149 RAFLPAGRLQLLKIITTDFNTLIASWKIRKFLIFTLICACSLRNEVSGGIILAPEGINGS 208
                                                        VSGGIILAPEGINGS
Sbjct: 149 --------------------------------------------RVSGGIILAPEGINGS 208

Query: 209 ICGTRESVQRVLGFIESDERLKGLRQVETPVSSEEEAIHHGHTSSSPLAAGEDAPFRWDH 268
           ICG RESV+ VL FI+ D RL GLRQVETPVS E+EAIHHGH+SSSPLAAGEDAPFRWDH
Sbjct: 209 ICGIRESVEEVLAFIQRDVRLNGLRQVETPVSPEQEAIHHGHSSSSPLAAGEDAPFRWDH 268

Query: 269 VRVKLKKEIVTLGMPGISPVERVGKYVKPRDWNVLINDPDTVVIDVRNHYETRIGKFKRA 328
           VRVKLKKEIVTLG+P +SP+ERVG YV P +WN LI+DP+TVVIDVRN YETRIGKFK A
Sbjct: 269 VRVKLKKEIVTLGIPSVSPIERVGTYVSPEEWNELISDPETVVIDVRNTYETRIGKFKGA 328

Query: 329 VDPCTTAFRDFPVWVEDEFQLPNSDNKHSNQGVEEEAVN---------PEQNSLPRVAMY 388
           VDPCTTAFR+FP WVE++F L    N+ +   VE+E  +          +  +LPR+AMY
Sbjct: 329 VDPCTTAFRNFPSWVENQFALKQEGNE-TQAKVEKEDFSEITHKEDKAEKPKTLPRIAMY 388

Query: 389 CTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGL 448
           CTGGIRCEKASS LLS+GF+EVYHL+GGILKYLEEVPKTESLWEGECFVFDKRVSVEHGL
Sbjct: 389 CTGGIRCEKASSLLLSQGFEEVYHLKGGILKYLEEVPKTESLWEGECFVFDKRVSVEHGL 448

Query: 449 EQGTFKLCYGCKQPVSDADMESPEWEYGVSCPHCYSTKSEEEKERARARQRQFETWGIIG 501
            QGT KLCYGCKQP+SD DME+PE+EYGVSCP+CYS KSEEEKERARARQ QFE WG+IG
Sbjct: 449 AQGTHKLCYGCKQPISDEDMEAPEYEYGVSCPYCYSKKSEEEKERARARQTQFEEWGVIG 455

BLAST of Lag0041056 vs. TAIR 10
Match: AT1G09280.1 (CONTAINS InterPro DOMAIN/s: Rhodanese-like (InterPro:IPR001763), Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: Rhodanese/Cell cycle control phosphatase superfamily protein (TAIR:AT2G40760.1); Has 5925 Blast hits to 5912 proteins in 1592 species: Archae - 0; Bacteria - 2946; Metazoa - 156; Fungi - 408; Plants - 229; Viruses - 0; Other Eukaryotes - 2186 (source: NCBI BLink). )

HSP 1 Score: 105.1 bits (261), Expect = 1.7e-22
Identity = 76/270 (28.15%), Postives = 129/270 (47.78%), Query Frame = 0

Query: 189 GGIILAPEGINGSICGTRESVQRVLGFIESDERLKGLRQVETPVSSEEEAIHHGHTSSSP 248
           G + L+P+G+N ++ G   +++  +   +S+   +G          +  + HH      P
Sbjct: 52  GRVRLSPKGVNVTVGGKLTALEEHIAAAKSNCLFEG-------TDFKLASCHH------P 111

Query: 249 LAAGEDAPFRWDHVRVKLKKEIVTLG-MPGISP--VERVGKYVKPRDWNVLI-------N 308
           L         +  + +++ +E+VT    P + P  +   GK++   +++ ++        
Sbjct: 112 LNDKVAEECGFTSLSIRVVEELVTFSPCPPLKPPEISNAGKHLSAAEFHSVLQSANGKSE 171

Query: 309 DPDTVVIDVRNHYETRIGKFK----RAVDPCTTAFRDFPVWVEDEFQLPNSDNKHSNQGV 368
           + + V++D RN YETRIGKF+      +DP    + D P W++                 
Sbjct: 172 NKELVLLDARNLYETRIGKFESENVETLDPEIRQYSDLPTWIDQ---------------- 231

Query: 369 EEEAVNPEQNSLPRVAMYCTGGIRCEKASSYLLSK--GFKEVYHLEGGILKYLEEVPKTE 428
                N E+     V MYCTGGIRCE AS+Y+ SK  GF+  + L GGI +YLE+ P + 
Sbjct: 232 -----NAEKMKGKNVLMYCTGGIRCEMASAYIRSKGAGFENTFQLYGGIQRYLEQFP-SG 286

Query: 429 SLWEGECFVFDKRVSVEHGLEQ--GTFKLC 441
             ++G+ FVFD R+SV    E   G+  LC
Sbjct: 292 GFFKGKNFVFDHRISVGSSKEDIIGSCLLC 286

BLAST of Lag0041056 vs. TAIR 10
Match: AT1G17850.1 (Rhodanese/Cell cycle control phosphatase superfamily protein )

HSP 1 Score: 89.4 bits (220), Expect = 9.4e-18
Identity = 77/316 (24.37%), Postives = 124/316 (39.24%), Query Frame = 0

Query: 187 VSGGIILAPEGINGSICGTRESVQRVLGFIESDERLKGLRQVETPVSS----------EE 246
           + G I L  +GIN    G  +     + +++ D+R   L    +P  +           +
Sbjct: 113 IRGRIYLNEQGINAQYSGPSKDALAYVEWLKGDDRFSDLLVQMSPAMNRHAFPKLKLQNK 172

Query: 247 EAIHHGHTSSSPLAAGEDAPFRWDHVRVKLKKEIVTLGMPGISPVERVGKYVKPRDWNVL 306
            +++ G  S  PL    D P R                           K ++P +W   
Sbjct: 173 PSLYEGGISHLPLL---DPPMR--------------------------AKPLEPSEWKRK 232

Query: 307 IND-------------PDTVVIDVRNHYETRIGKFKRAVDPCTTAFRDFPVWVEDEFQLP 366
           + D                +++DVRN YE  +G F+ A  P    FR+    + DE + P
Sbjct: 233 LKDLTDDDEASPSNSGKSYILLDVRNGYEWDVGHFRGAHRPEVDCFRNTSFGLSDEKEAP 292

Query: 367 NSDNKHSNQGVEEEAVNPEQNSLPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILK 426
           +           +  +N ++     + MYCTGGIRC+  S+ L  +GFK +Y L+GG+  
Sbjct: 293 S-----------DPLINVDKEKTD-ILMYCTGGIRCDVYSTVLRQRGFKNLYTLKGGVSH 352

Query: 427 YLEEVPKTESLWEGECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSC 480
           YL+E    E  W G  FVFD R+S+          +       V D    +P+     S 
Sbjct: 353 YLKEEGTAE--WVGNLFVFDSRLSLPPAAYNDNV-VDKAVGDNVVDEAGRTPQTPVDTSF 384

BLAST of Lag0041056 vs. TAIR 10
Match: AT1G17850.2 (Rhodanese/Cell cycle control phosphatase superfamily protein )

HSP 1 Score: 89.4 bits (220), Expect = 9.4e-18
Identity = 76/306 (24.84%), Postives = 126/306 (41.18%), Query Frame = 0

Query: 187 VSGGIILAPEGINGSICGTRESVQRVLGFIESDERLKGLRQVETPVSSEEEAIHHGHTSS 246
           + G I L  +GIN    G  +     + +++ D+R   L    +P  +           +
Sbjct: 113 IRGRIYLNEQGINAQYSGPSKDALAYVEWLKGDDRFSDLLVQMSPAMNRH---------A 172

Query: 247 SPLAAGEDAPFRWDHVRVKLKKEIVTLGMPGISPVERVGKYVKPRDWNVLIND------- 306
            P    ++ P         ++ E     +P + P  R  K ++P +W   + D       
Sbjct: 173 FPKLKLQNKP-------SLVQYEGGISHLPLLDPPMR-AKPLEPSEWKRKLKDLTDDDEA 232

Query: 307 ------PDTVVIDVRNHYETRIGKFKRAVDPCTTAFRDFPVWVEDEFQLPNSDNKHSNQG 366
                    +++DVRN YE  +G F+ A  P    FR+    + DE + P+         
Sbjct: 233 SPSNSGKSYILLDVRNGYEWDVGHFRGAHRPEVDCFRNTSFGLSDEKEAPS--------- 292

Query: 367 VEEEAVNPEQNSLPRVAMYCTGGIRCEKASSYLLSKGFKEVYHLEGGILKYLEEVPKTES 426
             +  +N ++     + MYCTGGIRC+  S+ L  +GFK +Y L+GG+  YL+E    E 
Sbjct: 293 --DPLINVDKEKTD-ILMYCTGGIRCDVYSTVLRQRGFKNLYTLKGGVSHYLKEEGTAE- 352

Query: 427 LWEGECFVFDKRVSVEHGLEQGTFKLCYGCKQPVSDADMESPEWEYGVSCPHCYSTKSEE 480
            W G  FVFD R+S+          +       V D    +P+     S   CY   S+ 
Sbjct: 353 -WVGNLFVFDSRLSLPPAAYNDNV-VDKAVGDNVVDEAGRTPQTPVDTSFARCYLCNSQV 386