Lag0041028 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0041028
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Methylenetetrahydrofolate reductase
Location	chr13: 11049925 .. 11050520 (-)
RNA-Seq Expression	Lag0041028
Synteny	Lag0041028

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGAATGGGCTGAATGGAATCCTTGGCTTGGTAAGACACTTCAAACTATTGACTTCCTTGCAACCAAATGACAATTAAAAGGTAATATTTATAAGACACTCATCTTAAAACTCTAACTTGTTTTAAAGTGTTGGATTAGATTAAGTGTTAAGAGTGTTATGCTTATCGACAGAGAAAATGACATTAGATAGATTTGAAGGCATGAAACTTGTTCTAATATCGTATTGAATTCTATTGAGATAAAATATTTAACTAATGGGTTACAATCCAGCTATTGTCTGTCACTTTAACTCACGTTATTCAGAAATATCATTTAATTTCACGTCGTTGCTTTTAAAAATTCTATGTAATTTCTTACAAATTTTCATTTTTCTTCTCTAAAGAAAAATTACTTAGATCTATAGTTAAATTGTTTCTGTTTTACCTGTAATGCATGACTTTAGGAAACTTGCACATTATTCCAGATGAGATCATGACTGCCTTAAAGCCTATCAAGGACAATGAAGAAGTTAAAGTTTATGGAATTCACCTTGGAACTGAGATGTGCAAGAAGATTTTAGTGGAATCAAGACTCTACATCTTTATGCATTAA

mRNA sequence

ATGGCAGAATGGGCTGAATGGAATCCTTGGCTTGGAAACTTGCACATTATTCCAGATGAGATCATGACTGCCTTAAAGCCTATCAAGGACAATGAAGAAGTTAAAGTTTATGGAATTCACCTTGGAACTGAGATGTGCAAGAAGATTTTAGTGGAATCAAGACTCTACATCTTTATGCATTAA

Coding sequence (CDS)

ATGGCAGAATGGGCTGAATGGAATCCTTGGCTTGGAAACTTGCACATTATTCCAGATGAGATCATGACTGCCTTAAAGCCTATCAAGGACAATGAAGAAGTTAAAGTTTATGGAATTCACCTTGGAACTGAGATGTGCAAGAAGATTTTAGTGGAATCAAGACTCTACATCTTTATGCATTAA

Protein sequence

MAEWAEWNPWLGNLHIIPDEIMTALKPIKDNEEVKVYGIHLGTEMCKKILVESRLYIFMH

Homology

[Close]

BLAST of Lag0041028 vs. NCBI nr
Match: XP_004250093.1 (methylenetetrahydrofolate reductase 2 [Solanum lycopersicum])

HSP 1 Score: 64.3 bits (155), Expect = 4.1e-07
Identity = 31/35 (88.57%), Postives = 32/35 (91.43%), Query Frame = 0


Query: 17  IPDEIMTALKPIKDNEE-VKVYGIHLGTEMCKKIL 51
           IPDEIM AL+PIKDNEE VK YGIHLGTEMCKKIL
Sbjct: 241 IPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKIL 275

[Close]

BLAST of Lag0041028 vs. NCBI nr
Match: KAA0060205.1 (methylenetetrahydrofolate reductase 2 [Cucumis melo var. makuwa] >TYJ95579.1 methylenetetrahydrofolate reductase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 64.3 bits (155), Expect = 4.1e-07
Identity = 31/35 (88.57%), Postives = 32/35 (91.43%), Query Frame = 0


Query: 17  IPDEIMTALKPIKDNEE-VKVYGIHLGTEMCKKIL 51
           IPDEIM AL+PIKDNEE VK YGIHLGTEMCKKIL
Sbjct: 243 IPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKIL 277

[Close]

BLAST of Lag0041028 vs. NCBI nr
Match: XP_022155988.1 (methylenetetrahydrofolate reductase 2-like [Momordica charantia])

HSP 1 Score: 64.3 bits (155), Expect = 4.1e-07
Identity = 31/35 (88.57%), Postives = 32/35 (91.43%), Query Frame = 0


Query: 17  IPDEIMTALKPIKDNEE-VKVYGIHLGTEMCKKIL 51
           IPDEIM AL+PIKDNEE VK YGIHLGTEMCKKIL
Sbjct: 243 IPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKIL 277

[Close]

BLAST of Lag0041028 vs. NCBI nr
Match: XP_038874836.1 (methylenetetrahydrofolate reductase 2-like [Benincasa hispida] >XP_038874837.1 methylenetetrahydrofolate reductase 2-like [Benincasa hispida])

HSP 1 Score: 64.3 bits (155), Expect = 4.1e-07
Identity = 31/35 (88.57%), Postives = 32/35 (91.43%), Query Frame = 0


Query: 17  IPDEIMTALKPIKDNEE-VKVYGIHLGTEMCKKIL 51
           IPDEIM AL+PIKDNEE VK YGIHLGTEMCKKIL
Sbjct: 243 IPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKIL 277

[Close]

BLAST of Lag0041028 vs. NCBI nr
Match: XP_008457198.1 (PREDICTED: probable methylenetetrahydrofolate reductase [Cucumis melo] >XP_008457199.1 PREDICTED: probable methylenetetrahydrofolate reductase [Cucumis melo])

HSP 1 Score: 64.3 bits (155), Expect = 4.1e-07
Identity = 31/35 (88.57%), Postives = 32/35 (91.43%), Query Frame = 0


Query: 17  IPDEIMTALKPIKDNEE-VKVYGIHLGTEMCKKIL 51
           IPDEIM AL+PIKDNEE VK YGIHLGTEMCKKIL
Sbjct: 243 IPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKIL 277

[Close]

BLAST of Lag0041028 vs. ExPASy Swiss-Prot
Match: O80585 (Methylenetetrahydrofolate reductase 2 OS=Arabidopsis thaliana OX=3702 GN=MTHFR2 PE=1 SV=2)

HSP 1 Score: 59.3 bits (142), Expect = 1.7e-08
Identity = 28/35 (80.00%), Postives = 31/35 (88.57%), Query Frame = 0


Query: 17  IPDEIMTALKPIKDNEE-VKVYGIHLGTEMCKKIL 51
           IP E+M AL+PIKDNEE VK YGIHLGTEMCKK+L
Sbjct: 241 IPVEVMAALEPIKDNEEAVKAYGIHLGTEMCKKML 275

[Close]

BLAST of Lag0041028 vs. ExPASy Swiss-Prot
Match: Q75HE6 (Probable methylenetetrahydrofolate reductase OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0815200 PE=2 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 2.2e-08
Identity = 29/35 (82.86%), Postives = 30/35 (85.71%), Query Frame = 0


Query: 17  IPDEIMTALKPIKDNEE-VKVYGIHLGTEMCKKIL 51
           IP EI  AL+PIKDNEE VK YGIHLGTEMCKKIL
Sbjct: 241 IPAEITAALEPIKDNEEAVKAYGIHLGTEMCKKIL 275

[Close]

BLAST of Lag0041028 vs. ExPASy Swiss-Prot
Match: Q9SE94 (Methylenetetrahydrofolate reductase 1 OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 8.5e-08
Identity = 28/35 (80.00%), Postives = 29/35 (82.86%), Query Frame = 0


Query: 17  IPDEIMTALKPIKDNEE-VKVYGIHLGTEMCKKIL 51
           IP EI  AL PIKDNEE V+ YGIHLGTEMCKKIL
Sbjct: 241 IPSEITAALDPIKDNEEAVRQYGIHLGTEMCKKIL 275

[Close]

BLAST of Lag0041028 vs. ExPASy Swiss-Prot
Match: Q9SE60 (Methylenetetrahydrofolate reductase 1 OS=Arabidopsis thaliana OX=3702 GN=MTHFR1 PE=1 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 1.2e-06
Identity = 25/35 (71.43%), Postives = 28/35 (80.00%), Query Frame = 0


Query: 17  IPDEIMTALKPIKDNEE-VKVYGIHLGTEMCKKIL 51
           IP E+  AL+PIKDN+E VK YGIH  TEMCKKIL
Sbjct: 241 IPAELTAALEPIKDNDEAVKAYGIHFATEMCKKIL 275

The following BLAST results are available for this feature:

BLAST of Lag0041028 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004250093.1	4.1e-07	88.57	methylenetetrahydrofolate reductase 2 [Solanum lycopersicum]	[more]
KAA0060205.1	4.1e-07	88.57	methylenetetrahydrofolate reductase 2 [Cucumis melo var. makuwa] >TYJ95579.1 met...	[more]
XP_022155988.1	4.1e-07	88.57	methylenetetrahydrofolate reductase 2-like [Momordica charantia]	[more]
XP_038874836.1	4.1e-07	88.57	methylenetetrahydrofolate reductase 2-like [Benincasa hispida] >XP_038874837.1 m...	[more]
XP_008457198.1	4.1e-07	88.57	PREDICTED: probable methylenetetrahydrofolate reductase [Cucumis melo] >XP_00845...	[more]

BLAST of Lag0041028 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O80585	1.7e-08	80.00	Methylenetetrahydrofolate reductase 2 OS=Arabidopsis thaliana OX=3702 GN=MTHFR2 ...	[more]
Q75HE6	2.2e-08	82.86	Probable methylenetetrahydrofolate reductase OS=Oryza sativa subsp. japonica OX=...	[more]
Q9SE94	8.5e-08	80.00	Methylenetetrahydrofolate reductase 1 OS=Zea mays OX=4577 PE=2 SV=1	[more]
Q9SE60	1.2e-06	71.43	Methylenetetrahydrofolate reductase 1 OS=Arabidopsis thaliana OX=3702 GN=MTHFR1 ...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003171	Methylenetetrahydrofolate reductase-like	PFAM	PF02219	MTHFR	coord: 17..53 e-value: 4.9E-6 score: 26.1

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0041028.1	Lag0041028.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009086	methionine biosynthetic process
biological_process	GO:0035999	tetrahydrofolate interconversion
biological_process	GO:0006555	methionine metabolic process
cellular_component	GO:0005829	cytosol
molecular_function	GO:0071949	FAD binding
molecular_function	GO:0106312	methylenetetrahydrofolate reductase NADH activity
molecular_function	GO:0106313	methylenetetrahydrofolate reductase NADPH activity
molecular_function	GO:0004489	methylenetetrahydrofolate reductase (NAD(P)H) activity