Lag0041027 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0041027
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptiontRNA (guanine(26)-N(2))-dimethyltransferase
Locationchr13: 11039608 .. 11042629 (+)
RNA-Seq ExpressionLag0041027
SyntenyLag0041027
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTAACTGTAACGCCTAAAACCCTATCCCCTTCCCCATTTCTTCACAGCCCACAAACAAATTCCCATTGCTTCACATCTTCAACGATTCATCTCTTCCGATCTTCAAAGTGGACGAAAAAATCTCTCAATAATTGTCTCTCAAACAATCGCACCGAAAGGGGCGTAGAATTCGACATCGGAGACACCTTCTTCCGCCACGAAAGCGCCACCGGACGCGACTTGGGCGTTCTCGCGGCGGCGCTCTACCGGAAATCCAAGGGCAGTATTCGGGTTCTCGACGCATTGTGCGGTTGTGGGATCCGGTCGCTTCGGTACTTGGTCGAGGCCGAGGCTGATTTTTTGTGGGCGAATGATGCTAATGACGAAAATCGGAGGGTTATAGTGAAGAATCTATCGAGGGTTGGGAGAGGTTTTGGGGATGACCGGCGGTGGGTGGTTACGAATTGTGATGCGAACAGGGTTATGACGGAGATTTATTTGCACAAGGATTTTTTTGATTTTATTGATGTTGATTCCTTTGGGAGTGATTCGACGTTCTTGAGGTCTGCTATTAATGCGTTGAAATTGGGTGGGTTGCTCTATGTTACGTCGACGGATGGTTTCTCTTCCGGTGGTCATCGACCTTCTCAGTGAGTGCAGAATCTTATCTGTTGGAATTTGTACTCTGTTTCAGCTAGTTTGCTTTATGCCGCTTTATTAAAAATCTTGACATGAAACGACTGATCATTGTAATTGTCTTATATCTATATATAAAAGATTAAATATCACACTCTTTTAGTTTTGAGTTTTAGTTGTTTAATATTGTGTTAGCTCTCAGTTAATGTTTGATAGTTACTTGTTGATTCTGTGTGAGGGATGTAAGCAGTTCGGTTTTTTTTTTGGCCAAAACTGACTTTGAAACGACATATGGTTTAAGATAAATTTAAAACTGACATTGAAACCAATTTTTTTTTTTGAAAGCTGAACTGAGCTAGCTGAAATGACAGAACCAACCGAAATTGAACAAGGACAAAAGAAACCAACAAATATTTATCAAAATCTTTTTTGGTTTTGGCTTGCACCCTTGTACTAATTTTGAAGTTGACTAGTGAAACTGCTAAGTAGTAGTTTAGTAGTAAGAACATAGGTGGGTAATTATCTCAAGATGCATGTATACTATGGTTGGTTTGGTAATTCAAAATTCTTTTTTCCCTTCTTTCTCTTTTTTTTTTTTTTACCCCCCTCTAGATCGTTAGGTGCATATGGAGCATATGTGCGTCCCATGCCATTTTCGAATGAAATTGGTTTACGAATGCTTATAGGTGGGGTTGTACGTGAAGCTTCTGTACTGGGTTACTATGCAACGCCACTCTTTTCGTATTATTCATACCACGGACCTGTTTTCAGAGTTATGCTTCAAATCAATCGAGGGAAGATTTATAAAGACAGGTGATAGTTGATGAGTTTAGCATGTATGCTATTGCTGGATGTATCGTGTGTTCATTCATGGTGCTTGATTCAAGCTTTCACTACATATATCAGGCATTATGGTTTCATCAGCTATTGCGAAAAATGTGGAAATTCACAAGCTTTTTCATGGAGTGAGCTTGGGCAGATGAGTTGTCCTTGCAATGATTCAAGGGTATGACTATGCCATTCTGCTACCCTTTTGAGCTTAAGCATCCACTGTTTGAGCTTCCACATTTTAAACTGACACCAAAAGTCCCTTGTATAACTTGCATGGTCCAGATAAACACTGGCTCCAAGCTGAATATGCTTGAATTTACTGTTATCTTTTCTTCTGTATTAGGCTCTTTCTTTCTGATGTAAATACTTGTAACATTCTATTCTGTTAGTTCGGAATACATAAAATTTTTTACTTGTTAGGTTTCTGAATCACTTGTGGTTTCGGGACCTCTTTGGACTGGACCTCTACACAGTGCAGACTACATTGAAGAGATGTTATCCTTAGCTAAGCAGTGGGAATGGATAGGCAATGGCCAGGGAAAGGATCTTGAGAAACTTCTGCGGCAGATGGTGGATGAGAGTGACCCCAAACTACCAGTAGGGTACATCAAAATGGACGAGGTGGCCAAATTTTATTTATTCTTCTCGTGGTTTAAAATTTCATTTCTGTTTCTAGACCAACAATGTAAGAAGTGATCTCTCTATCCCCTTCTCTCTATGTTTAGGTGGCAAGTCGTGCAAAAGTAAATTCACCTCCCCTCAATACCATGATGAGCGAGATGATCAAGGTGAGAATTTTGGTAGTTAAGATGTATTAATTGTGTGACGCATGTGCTTGTCCACATTGGCTGAACTGTTCGTTGTCATGAGATTCTACTAAATGCTGGAAAGCAAAGAAAAATTGACACAGACATGACACATATACAACACAACTTGGATAAAATGACATGTTATTCCATATGAGTAAGGACCTAGACACGTTGAGGACATGTTTTCTATAATTTATATATTTTTAAACATATATCATTTTTATACTAGAAGTTCTATCGTCAATAATCTTATGCATTTATTTTCTTTAAAAAGACAAGTTTTGATGTATTTTAGTCTCAAAATTTATTACTTTTATCTTATATTACTTTTGCCTCTTTATTATGCTTAACACGTTTAACAAGTGTTTGACACATGTCTAATAGGTGTCAAACTCGTATTCAATTTGTTCAACTAGTGTCTGACATATGTTTATCGTGCTAATAAGTATCTAAAATGTGTTCAACACGTGTCGGAGTGTTTGATACGTATCGAGTGCAGACAGTTTATCCAAACTAGAGTGTTTAAGTTTCATAACTGGAAAGTGAATGGGTTTTTGCCTTGAATGTAGATGCTTGAAAAGAAAACAGTTCTCTCTCTTCTTTGCCTCTCTTTTTGAAGAGTTTGGTTGTTGCAGGCTGGATATGCTGCTAGTAGGTCTCATATTGCCTCAAACGCCATCAAGACAAACTGTCCAATGGCAGAGTGCATCAGAATTGCTCTAGAACTGCAGCAGAGTTGTCTTGATGTGCATCAAGTCGCATGA

mRNA sequence

ATGTTAACTGTAACGCCTAAAACCCTATCCCCTTCCCCATTTCTTCACAGCCCACAAACAAATTCCCATTGCTTCACATCTTCAACGATTCATCTCTTCCGATCTTCAAAGTGGACGAAAAAATCTCTCAATAATTGTCTCTCAAACAATCGCACCGAAAGGGGCGTAGAATTCGACATCGGAGACACCTTCTTCCGCCACGAAAGCGCCACCGGACGCGACTTGGGCGTTCTCGCGGCGGCGCTCTACCGGAAATCCAAGGGCAGTATTCGGGTTCTCGACGCATTGTGCGGTTGTGGGATCCGGTCGCTTCGGTACTTGGTCGAGGCCGAGGCTGATTTTTTGTGGGCGAATGATGCTAATGACGAAAATCGGAGGGTTATAGTGAAGAATCTATCGAGGGTTGGGAGAGGTTTTGGGGATGACCGGCGGTGGGTGGTTACGAATTGTGATGCGAACAGGGTTATGACGGAGATTTATTTGCACAAGGATTTTTTTGATTTTATTGATGTTGATTCCTTTGGGAGTGATTCGACGTTCTTGAGGTCTGCTATTAATGCGTTGAAATTGGGTGGGTTGCTCTATGTTACGTCGACGGATGGTTTCTCTTCCGGTGGTCATCGACCTTCTCAATCGTTAGGTGCATATGGAGCATATGTGCGTCCCATGCCATTTTCGAATGAAATTGGTTTACGAATGCTTATAGGTGGGGTTGTACGTGAAGCTTCTGTACTGGGTTACTATGCAACGCCACTCTTTTCGTATTATTCATACCACGGACCTGTTTTCAGAGTTATGCTTCAAATCAATCGAGGGAAGATTTATAAAGACAGGCATTATGGTTTCATCAGCTATTGCGAAAAATGTGGAAATTCACAAGCTTTTTCATGGAGTGAGCTTGGGCAGATGAGTTGTCCTTGCAATGATTCAAGGGTTTCTGAATCACTTGTGGTTTCGGGACCTCTTTGGACTGGACCTCTACACAGTGCAGACTACATTGAAGAGATGTTATCCTTAGCTAAGCAGTGGGAATGGATAGGCAATGGCCAGGGAAAGGATCTTGAGAAACTTCTGCGGCAGATGGTGGATGAGAGTGACCCCAAACTACCAGTAGGGTACATCAAAATGGACGAGGTGGCAAGTCGTGCAAAAGTAAATTCACCTCCCCTCAATACCATGATGAGCGAGATGATCAAGGCTGGATATGCTGCTAGTAGGTCTCATATTGCCTCAAACGCCATCAAGACAAACTGTCCAATGGCAGAGTGCATCAGAATTGCTCTAGAACTGCAGCAGAGTTGTCTTGATGTGCATCAAGTCGCATGA

Coding sequence (CDS)

ATGTTAACTGTAACGCCTAAAACCCTATCCCCTTCCCCATTTCTTCACAGCCCACAAACAAATTCCCATTGCTTCACATCTTCAACGATTCATCTCTTCCGATCTTCAAAGTGGACGAAAAAATCTCTCAATAATTGTCTCTCAAACAATCGCACCGAAAGGGGCGTAGAATTCGACATCGGAGACACCTTCTTCCGCCACGAAAGCGCCACCGGACGCGACTTGGGCGTTCTCGCGGCGGCGCTCTACCGGAAATCCAAGGGCAGTATTCGGGTTCTCGACGCATTGTGCGGTTGTGGGATCCGGTCGCTTCGGTACTTGGTCGAGGCCGAGGCTGATTTTTTGTGGGCGAATGATGCTAATGACGAAAATCGGAGGGTTATAGTGAAGAATCTATCGAGGGTTGGGAGAGGTTTTGGGGATGACCGGCGGTGGGTGGTTACGAATTGTGATGCGAACAGGGTTATGACGGAGATTTATTTGCACAAGGATTTTTTTGATTTTATTGATGTTGATTCCTTTGGGAGTGATTCGACGTTCTTGAGGTCTGCTATTAATGCGTTGAAATTGGGTGGGTTGCTCTATGTTACGTCGACGGATGGTTTCTCTTCCGGTGGTCATCGACCTTCTCAATCGTTAGGTGCATATGGAGCATATGTGCGTCCCATGCCATTTTCGAATGAAATTGGTTTACGAATGCTTATAGGTGGGGTTGTACGTGAAGCTTCTGTACTGGGTTACTATGCAACGCCACTCTTTTCGTATTATTCATACCACGGACCTGTTTTCAGAGTTATGCTTCAAATCAATCGAGGGAAGATTTATAAAGACAGGCATTATGGTTTCATCAGCTATTGCGAAAAATGTGGAAATTCACAAGCTTTTTCATGGAGTGAGCTTGGGCAGATGAGTTGTCCTTGCAATGATTCAAGGGTTTCTGAATCACTTGTGGTTTCGGGACCTCTTTGGACTGGACCTCTACACAGTGCAGACTACATTGAAGAGATGTTATCCTTAGCTAAGCAGTGGGAATGGATAGGCAATGGCCAGGGAAAGGATCTTGAGAAACTTCTGCGGCAGATGGTGGATGAGAGTGACCCCAAACTACCAGTAGGGTACATCAAAATGGACGAGGTGGCAAGTCGTGCAAAAGTAAATTCACCTCCCCTCAATACCATGATGAGCGAGATGATCAAGGCTGGATATGCTGCTAGTAGGTCTCATATTGCCTCAAACGCCATCAAGACAAACTGTCCAATGGCAGAGTGCATCAGAATTGCTCTAGAACTGCAGCAGAGTTGTCTTGATGTGCATCAAGTCGCATGA

Protein sequence

MLTVTPKTLSPSPFLHSPQTNSHCFTSSTIHLFRSSKWTKKSLNNCLSNNRTERGVEFDIGDTFFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLWANDANDENRRVIVKNLSRVGRGFGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDVDSFGSDSTFLRSAINALKLGGLLYVTSTDGFSSGGHRPSQSLGAYGAYVRPMPFSNEIGLRMLIGGVVREASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKDRHYGFISYCEKCGNSQAFSWSELGQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQGKDLEKLLRQMVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPMAECIRIALELQQSCLDVHQVA
Homology
[Close]
BLAST of Lag0041027 vs. NCBI nr
Match: XP_038892632.1 (tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Benincasa hispida])

HSP 1 Score: 794.7 bits (2051), Expect = 4.2e-226
Identity = 390/440 (88.64%), Postives = 410/440 (93.18%), Query Frame = 0 

Query: 1   MLTVTPKTLSPSPFLHSPQTNSHCFTSSTIHLFRSSKWTKKSLNNCLSNNRTERGVEFDI 60
           ML+V P+T SPSPFLHSPQTNSH  ++STIHLF+ S   K SLNNCL NNRTERG+EFDI
Sbjct: 1   MLSVLPRTPSPSPFLHSPQTNSHSLSASTIHLFQFSHSNKNSLNNCLPNNRTERGLEFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLWANDA 120
           G TFFRHESATGRDLGVLAAALYRKSKG +RVLDALCGCGIRSLRYLVEAEADF+ ANDA
Sbjct: 61  GGTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 121 NDENRRVIVKNLSRVGRGFGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDVDSFGSDSTF 180
           NDENRRVIVKNLSRV    GD RRW+VTNCDANRVMT  YL KDFFDFID+DSFGSDS+F
Sbjct: 121 NDENRRVIVKNLSRVAEDSGDARRWMVTNCDANRVMTGFYLQKDFFDFIDIDSFGSDSSF 180

Query: 181 LRSAINALKLGGLLYVTSTDGFSSGGHRPSQSLGAYGAYVRPMPFSNEIGLRMLIGGVVR 240
           LR AINALKL GLLYVTSTDGFSSGGHRPSQSL AYGAYVRPMPFSNEIGLRMLIGGV R
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKDRHYGFISYCEKCGNSQAFSWSEL 300
           EASVLG+YATPLFSYYSYHGPVFRVML+INRG++YK+RHYGFISYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGHYATPLFSYYSYHGPVFRVMLRINRGRVYKNRHYGFISYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQGKDLEKLLRQ 360
           GQMSCPCNDSRVS+SLVVSGPLWTGPLHSADYIE MLSLAKQW+WIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSDSLVVSGPLWTGPLHSADYIENMLSLAKQWKWIGNGQGKDLEKLLGQ 360

Query: 361 MVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           M+DESD KLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MMDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELQQSCLDVHQV 441
           AECIRIALELQQ+CL  HQV
Sbjct: 421 AECIRIALELQQNCLGFHQV 440

[Close]
BLAST of Lag0041027 vs. NCBI nr
Match: XP_022943113.1 (tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita moschata])

HSP 1 Score: 794.3 bits (2050), Expect = 5.5e-226
Identity = 390/439 (88.84%), Postives = 410/439 (93.39%), Query Frame = 0 

Query: 1   MLTVTPKTLSPSPFLHSPQTNSHCFTSSTIHLFRSSKWTKKSLNNCLSNNRTERGVEFDI 60
           MLTV   TLSPSPFL + QT SHCF+SSTIHLF+ S+  KK LNNCL NNRTERG+EFDI
Sbjct: 1   MLTVMLNTLSPSPFLRTQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLWANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKGS+RVLDALCGCGIRSLRYLVEAEADF+ ANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 121 NDENRRVIVKNLSRVGRGFGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDVDSFGSDSTF 180
           NDENR VIVKNLSRV  GFG+ RRW+VTNCDANRVM+E YL KDFFDFIDVDSFGSDSTF
Sbjct: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180

Query: 181 LRSAINALKLGGLLYVTSTDGFSSGGHRPSQSLGAYGAYVRPMPFSNEIGLRMLIGGVVR 240
           LR AINALKL GLLYVTSTDGFSSGGH PSQSL AYGAYVRPMPFSNEIGLRMLIGGV R
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKDRHYGFISYCEKCGNSQAFSWSEL 300
           EASVLG+Y TPLFSYYSYHGPVFRVML+INRG+I K+RHYGF+SYC+KCGNSQAFSWSE+
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEV 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQGKDLEKLLRQ 360
           GQMSCPCND+RVSESLVVSGPLWTGPLHSADYIE+ML+LA QWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360

Query: 361 MVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MVDESD KLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELQQSCLDVHQ 440
           AECIRIALELQQSCL VHQ
Sbjct: 421 AECIRIALELQQSCLGVHQ 439

[Close]
BLAST of Lag0041027 vs. NCBI nr
Match: XP_022986521.1 (tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita maxima])

HSP 1 Score: 793.1 bits (2047), Expect = 1.2e-225
Identity = 390/440 (88.64%), Postives = 410/440 (93.18%), Query Frame = 0 

Query: 1   MLTVTPKTLSPSPFLHSPQTNSHCFTSSTIHLFRSSKWTKKSLNNCLSNNRTERGVEFDI 60
           MLTV   TLSPSPFL S QT SHCF+SSTIHLF+ S+  KK LNNCL NNRTERG+EFDI
Sbjct: 1   MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLWANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKGS+RVLDALCGCGIRSLRYLVEAEADF+ ANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 121 NDENRRVIVKNLSRVGRGFGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDVDSFGSDSTF 180
           NDENR VIVKNLSRV  GFG+ RRW+VTNCDANRVM+E YL KDFFDFIDVDSFGSDSTF
Sbjct: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180

Query: 181 LRSAINALKLGGLLYVTSTDGFSSGGHRPSQSLGAYGAYVRPMPFSNEIGLRMLIGGVVR 240
           LR AINALKL GLLYVTSTDGFSSGGH PSQSL AYGAYVRPMPFSNEIGLRMLIGGV R
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKDRHYGFISYCEKCGNSQAFSWSEL 300
           EASVLG+Y TPLFSYYSYHGPVFRVML+I+RG+I K+RHYGF+SYC+KCGNSQ FSWSE+
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEV 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQGKDLEKLLRQ 360
           GQMSCPCND+RVSESLVVSGPLWTGPLHSADYIE+ML+LA QWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360

Query: 361 MVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MVDESD KLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELQQSCLDVHQV 441
           AECIRIALELQQSCL VHQV
Sbjct: 421 AECIRIALELQQSCLGVHQV 440

[Close]
BLAST of Lag0041027 vs. NCBI nr
Match: XP_011655038.1 (tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus] >XP_011655039.1 tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus] >KGN50729.1 hypothetical protein Csa_005829 [Cucumis sativus])

HSP 1 Score: 792.7 bits (2046), Expect = 1.6e-225
Identity = 389/440 (88.41%), Postives = 413/440 (93.86%), Query Frame = 0 

Query: 1   MLTVTPKTLSPSPFLHSPQTNSHCFTSSTIHLFRSSKWTKKSLNNCLSNNRTERGVEFDI 60
           MLTV P+TLSPSPFLH PQTNSH  TSS+IHLF+ S   K  LNNCLSNNRTERG++FDI
Sbjct: 1   MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLWANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG +RVLDALCGCGIRSLRYLVE+EADF+ ANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENRRVIVKNLSRVGRGFGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDVDSFGSDSTF 180
           NDENRRVIVKNLSRV    GD+RRW+VTNCDANRVMTE YL KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRSAINALKLGGLLYVTSTDGFSSGGHRPSQSLGAYGAYVRPMPFSNEIGLRMLIGGVVR 240
           LR AINALKL GLLYVTSTDGFSSGGHRPSQSL AYGAYVRPMPFSNEIGLRMLIGGV R
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKDRHYGFISYCEKCGNSQAFSWSEL 300
           EASVLG+YATPLFSYYSYHGPVFRVML+INRG+I+K+RHYGF+SYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQGKDLEKLLRQ 360
           GQMSCPCNDSRVSESLVVSGPLW GPLHSADYIE++L+LAKQWEWIGNGQGKDLEKLLRQ
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 360

Query: 361 MVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MVDESD KLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELQQSCLDVHQV 441
           AECIRIA +LQ++CL  HQV
Sbjct: 421 AECIRIA-QLQRNCLGAHQV 439

[Close]
BLAST of Lag0041027 vs. NCBI nr
Match: XP_023529370.1 (tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 790.8 bits (2041), Expect = 6.0e-225
Identity = 389/440 (88.41%), Postives = 410/440 (93.18%), Query Frame = 0 

Query: 1   MLTVTPKTLSPSPFLHSPQTNSHCFTSSTIHLFRSSKWTKKSLNNCLSNNRTERGVEFDI 60
           MLTV   TLSPSPFL S QT SHCF+SSTIHLF+ S+   KSLNNCL NNRTERG+EFDI
Sbjct: 1   MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQIKSLNNCLPNNRTERGIEFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLWANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKGS+RVLDALCGCGIRSLRYLVEAEADF+ ANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 121 NDENRRVIVKNLSRVGRGFGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDVDSFGSDSTF 180
           NDENR VIVKNLS+V  GFG+ RRW+VTNCDANRVM+E YL KDFFDFIDVDSFGSDSTF
Sbjct: 121 NDENRGVIVKNLSKVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180

Query: 181 LRSAINALKLGGLLYVTSTDGFSSGGHRPSQSLGAYGAYVRPMPFSNEIGLRMLIGGVVR 240
           LR AINALKL GLLYVTSTDGFSSGGH PSQSL AYGAYVRPMPFSNEIGLRMLIGGV R
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKDRHYGFISYCEKCGNSQAFSWSEL 300
           EASVLG+Y TPLFSYYSYHGPVFRVML+I+RG+I K+RHYGF+SYC+KCGNSQAFSWSE+
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQAFSWSEV 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQGKDLEKLLRQ 360
           GQMSCPCND+RVSESLVVSGPLWTGPLHSADYIE+ML+LA QWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360

Query: 361 MVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MVDESD KLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELQQSCLDVHQV 441
            ECIRIALELQQSCL VHQV
Sbjct: 421 TECIRIALELQQSCLGVHQV 440

[Close]
BLAST of Lag0041027 vs. ExPASy Swiss-Prot
Match: B6YUU9 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus onnurineus (strain NA1) OX=523850 GN=trm1 PE=3 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 9.9e-37
Identity = 125/379 (32.98%), Postives = 184/379 (48.55%), Query Frame = 0 

Query: 64  FFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLWANDANDE 123
           F+    A  RD+ VLA     K+ GS RVLDAL   GIR +RY +E  A  +W ND N  
Sbjct: 27  FYNPLMALNRDISVLAL----KALGSRRVLDALSATGIRGIRYALETPAGEIWLNDINPN 86

Query: 124 NRRVIVKNLSRVGRG----------FGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDVDS 183
             R+IV N+     G             ++  VVTN DANR+M E +    +FDF+D+D 
Sbjct: 87  AFRLIVDNVKLNFSGELEIEGKMAVLEGEKTLVVTNADANRLMNEQF---RYFDFVDLDP 146

Query: 184 FGSDSTFLRSAINALKLGGLLYVTSTD-GFSSGGHRPSQSLGAYGAYVRPMPFSNEIGLR 243
           FGS   FL SA+ A+K  G+L +T+TD G   G +R +  L  Y A        +E GLR
Sbjct: 147 FGSPMEFLDSALRAVKRRGVLAITATDTGVLCGAYR-NACLRKYLAEPIRGELCHEAGLR 206

Query: 244 MLIGGVVREASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKDRHYGFISYCEKCGNS 303
           +LIG +VR A+        L +YY  H   FR  LQ+  G    D+    + Y  +    
Sbjct: 207 ILIGTLVRYAAKYDLGVEVLLAYYRDH--YFRAFLQLRNGAKTADKSMKQLGYLYQ---- 266

Query: 304 QAFSWSELGQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQGK 363
                 E G+      ++ +       GPLW GPL + +++EE+  L+ +   +G+   K
Sbjct: 267 -----DESGKFE--FENTFLPSKATAHGPLWLGPLKNQEFVEELHRLSAEHP-LGS---K 326

Query: 364 DLEKLLRQMVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASN 423
             +K L  + DE +  +P  Y     VA R  +    L  ++  + + GY A+R+H +  
Sbjct: 327 KTQKFLGLLKDELE--VPFFY-DTHAVARRHGLEVRKLAGVIEILYEKGYKATRTHFSPT 377

Query: 424 AIKTNCPMAECIRIALELQ 432
           A+KT+ P  E +    ELQ
Sbjct: 387 ALKTDAPFEEVLEALRELQ 377

[Close]
BLAST of Lag0041027 vs. ExPASy Swiss-Prot
Match: P81554 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) OX=186497 GN=trm1 PE=1 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 1.7e-33
Identity = 114/369 (30.89%), Postives = 173/369 (46.88%), Query Frame = 0 

Query: 64  FFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLWANDANDE 123
           F+    A  RD+ VL   L    K  I VLDAL   GIR +R+ +E  A+ +W ND N+ 
Sbjct: 29  FYNPRMAPNRDVVVL---LLNVLKPKI-VLDALSATGIRGIRFALETPAEEIWMNDINEL 88

Query: 124 NRRVIVKNLSRVGRG----------FGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDVDS 183
              ++ KN+    +G          F  ++  V+ N DANR+M E +    +F FID+D 
Sbjct: 89  AYELMKKNVLLNFKGTLKENAKRAIFEGEKTIVINNDDANRLMAEKH---RYFHFIDLDP 148

Query: 184 FGSDSTFLRSAINALKLGGLLYVTSTDGFSSGGHRPSQSLGAYGAYVRPMPFSNEIGLRM 243
           FGS   FL +A+ ++K  G+L VT+TDG    G  P   L  Y A        +E+G R+
Sbjct: 149 FGSPMEFLDTALRSVKRKGILGVTATDGAPLCGAHPKACLRKYLAVPLRGELCHEVGTRI 208

Query: 244 LIGGVVREASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKD---RHYGFISYCEKCG 303
           L+G + R A+        L +YY  H   FR  +++  G    D    + G++ + EK G
Sbjct: 209 LVGVIARYAAKYDLGMEVLLAYYKDH--YFRAFVKLKDGAKKGDETLENLGYVYFDEKTG 268

Query: 304 NSQAFSWSELGQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQ 363
             +                S +S      GPLW GPL +   + EML L      + N  
Sbjct: 269 RFEV-------------EKSFLSTRPNAYGPLWLGPLKNEKVVGEMLELLVSGFEVAN-- 328

Query: 364 GKDLEKLLRQMVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIA 420
            +++ KLL  + +E D  +P+ Y     +  R K+    L  ++ E+   GY A+R+H +
Sbjct: 329 YREVLKLLHMLHEELD--IPLFY-DTHALGKRLKIEPKKLGEIIKELKSMGYEATRTHFS 370

[Close]
BLAST of Lag0041027 vs. ExPASy Swiss-Prot
Match: Q9V1P3 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus abyssi (strain GE5 / Orsay) OX=272844 GN=trm1 PE=3 SV=2)

HSP 1 Score: 143.7 bits (361), Expect = 5.1e-33
Identity = 108/369 (29.27%), Postives = 177/369 (47.97%), Query Frame = 0 

Query: 64  FFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLWANDANDE 123
           F+    A  RD+ V+   L    K  I VLDAL   GIR +R+ +E  A+ +W ND +++
Sbjct: 27  FYNPRMALNRDIAVV---LLNVLKPRI-VLDALSATGIRGIRFALETPAEEIWMNDISED 86

Query: 124 NRRVIVKNLSRVGRG----------FGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDVDS 183
              ++ KN+    +G             ++  VV + DANR+M E +    +F FID+D 
Sbjct: 87  AYNLMKKNVLLNFKGELEESNGRAVLKSEKTLVVNHDDANRLMAEKH---RYFHFIDLDP 146

Query: 184 FGSDSTFLRSAINALKLGGLLYVTSTDGFSSGGHRPSQSLGAYGAYVRPMPFSNEIGLRM 243
           FGS   FL +A+ ++K  G+L +T+TDG    G  P   +  Y A        +E+G R+
Sbjct: 147 FGSPMEFLDTALRSVKRKGILGITATDGAPLCGAHPKACMRKYLAVPLRGELCHEVGTRI 206

Query: 244 LIGGVVREASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKD---RHYGFISYCEKCG 303
           L+G V R A+        + +YY  H   FR  +++  G    D    + G+I + E  G
Sbjct: 207 LVGVVARYAAKYDLGIEVILAYYKDH--YFRAFIKLKDGARKGDESLENLGYIYFDESTG 266

Query: 304 NSQAFSWSELGQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQ 363
                   E+ +   P   +         GP+W GPL S + +EEML +++Q   +   +
Sbjct: 267 K------FEVERSFLPSKPN-------AYGPVWLGPLKSQEIVEEMLEISQQ---LSLAR 326

Query: 364 GKDLEKLLRQMVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIA 420
            K   KLL+ + DE D  L   +     +  R K+ +  +  +++E+   GY ASR+H +
Sbjct: 327 KKQAVKLLKILKDELDVPL---FYDTHGLGRRLKIEARKIEEIINELRSLGYRASRTHFS 367

[Close]
BLAST of Lag0041027 vs. ExPASy Swiss-Prot
Match: C5A6N2 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3) OX=593117 GN=trm1 PE=3 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.8e-30
Identity = 115/376 (30.59%), Postives = 178/376 (47.34%), Query Frame = 0 

Query: 64  FFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLWANDANDE 123
           F+    A  RD+ VLA     K+    RVLDAL   GIR LRY +E  A+ +W ND N++
Sbjct: 27  FYNPVMALNRDISVLAV----KALSPRRVLDALSATGIRGLRYSLETSAEEVWMNDINED 86

Query: 124 NRRVIVKNL------------SRVGRGFGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDV 183
             R+I+KNL             R   G   ++R VV   DAN++M+E +    +FDFID+
Sbjct: 87  AFRLILKNLELNFGSKPVLANGRAELGL-PEKRLVVNLGDANKLMSENF---RYFDFIDL 146

Query: 184 DSFGSDSTFLRSAINALKLGGLLYVTSTD-GFSSGGHRPSQSLGAYGAYVRPMPFSNEIG 243
           D FGS   FL SA+ +++  G L +T+TD G   G +R +         +R     +E G
Sbjct: 147 DPFGSPVEFLDSALRSVRRRGFLGLTATDTGVLCGAYRRACRRKYLAEPIRG-ELCHEAG 206

Query: 244 LRMLIGGVVREASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKDRHYGFISYCEKCG 303
           LR+LIG +VR A+        L +YY  H   FRV L++  G    D     + Y     
Sbjct: 207 LRILIGTIVRYAAKYDLGVEVLLAYYRDH--YFRVFLRLRSGAKKADESVELLGYL---- 266

Query: 304 NSQAFSW-SELGQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNG 363
                 W  E G+         +       GP+W GPL +  ++EE++ L K  E   + 
Sbjct: 267 ------WQDETGKFK--YERDFLPGKRGAYGPMWLGPLKNQAFLEELVRLTKTSE--ESI 326

Query: 364 QGKDLEKLLRQMVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHI 423
             +   + L  +V+E D  +P  Y     +A +  +    ++ ++  +   G+ A+R+H 
Sbjct: 327 SHRKTFQFLSLLVEELD--VPFHY-DTHALARKNGLEVVKISRVIETLHSLGFEATRTHF 374

Query: 424 ASNAIKTNCPMAECIR 426
           +  +IKTN P    +R
Sbjct: 387 SPTSIKTNAPFDVVLR 374

[Close]
BLAST of Lag0041027 vs. ExPASy Swiss-Prot
Match: Q5JIB3 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) OX=69014 GN=trm1 PE=3 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 4.0e-30
Identity = 112/370 (30.27%), Postives = 178/370 (48.11%), Query Frame = 0 

Query: 64  FFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLWANDANDE 123
           F+    +  RD+ VLA     K  G  RVLDAL   GIR +RY +E  A+ +W ND ++E
Sbjct: 27  FYNPVMSLNRDISVLAV----KVLGPKRVLDALSATGIRGIRYALETPAEEVWLNDISEE 86

Query: 124 NRRVIVKNLSRVGRG----FGD------DRRWVVTNCDANRVMTEIYLHKDFFDFIDVDS 183
              ++ KN S    G     GD      ++  V+   DANR+M E +    +FDF+D+D 
Sbjct: 87  AYGLMKKNASLNIDGELYEEGDRSYLWGEKLVVINKGDANRLMAENF---RYFDFLDLDP 146

Query: 184 FGSDSTFLRSAINALKLGGLLYVTSTD-GFSSGGHRPSQSLGAYGAYVRPMPFSNEIGLR 243
           FGS   FL +A+ +++  G+L VT+TD G   G +R +  L  Y A     P  +E GLR
Sbjct: 147 FGSPVEFLDTALRSVRRNGVLAVTATDTGVLCGAYR-NACLRKYLAEPIRGPLCHEAGLR 206

Query: 244 MLIGGVVREASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKDR---HYGFISYCEKC 303
           +LIG VVR A+        L +Y+  H   FR  L++  G    D+     GF+ + +K 
Sbjct: 207 ILIGTVVRYAAKYDLGVEVLLAYHRDH--YFRAFLKLKSGAKKADKSLSQLGFL-WADKS 266

Query: 304 GNSQAFSWSELGQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNG 363
           G  +                  + +    SGPLW GPL    ++EE+L  A+        
Sbjct: 267 GRFE-------------YRRGFLPDKPGASGPLWLGPLKDESFVEELLESARDHPL---- 326

Query: 364 QGKDLEKLLRQMVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHI 420
             K     L+ + +E D  +P  Y     +A    +  P L+ ++  + + G++A+++H 
Sbjct: 327 AHKKTLSFLQLISEELD--VPFHY-DTHTLARACSLTPPKLDVIIQRLRELGHSATKTHF 365

[Close]
BLAST of Lag0041027 vs. ExPASy TrEMBL
Match: A0A6J1FWD4 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111447941 PE=3 SV=1)

HSP 1 Score: 794.3 bits (2050), Expect = 2.6e-226
Identity = 390/439 (88.84%), Postives = 410/439 (93.39%), Query Frame = 0 

Query: 1   MLTVTPKTLSPSPFLHSPQTNSHCFTSSTIHLFRSSKWTKKSLNNCLSNNRTERGVEFDI 60
           MLTV   TLSPSPFL + QT SHCF+SSTIHLF+ S+  KK LNNCL NNRTERG+EFDI
Sbjct: 1   MLTVMLNTLSPSPFLRTQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLWANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKGS+RVLDALCGCGIRSLRYLVEAEADF+ ANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 121 NDENRRVIVKNLSRVGRGFGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDVDSFGSDSTF 180
           NDENR VIVKNLSRV  GFG+ RRW+VTNCDANRVM+E YL KDFFDFIDVDSFGSDSTF
Sbjct: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180

Query: 181 LRSAINALKLGGLLYVTSTDGFSSGGHRPSQSLGAYGAYVRPMPFSNEIGLRMLIGGVVR 240
           LR AINALKL GLLYVTSTDGFSSGGH PSQSL AYGAYVRPMPFSNEIGLRMLIGGV R
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKDRHYGFISYCEKCGNSQAFSWSEL 300
           EASVLG+Y TPLFSYYSYHGPVFRVML+INRG+I K+RHYGF+SYC+KCGNSQAFSWSE+
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEV 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQGKDLEKLLRQ 360
           GQMSCPCND+RVSESLVVSGPLWTGPLHSADYIE+ML+LA QWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360

Query: 361 MVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MVDESD KLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELQQSCLDVHQ 440
           AECIRIALELQQSCL VHQ
Sbjct: 421 AECIRIALELQQSCLGVHQ 439

[Close]
BLAST of Lag0041027 vs. ExPASy TrEMBL
Match: A0A6J1JBD3 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111484232 PE=3 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 5.9e-226
Identity = 390/440 (88.64%), Postives = 410/440 (93.18%), Query Frame = 0 

Query: 1   MLTVTPKTLSPSPFLHSPQTNSHCFTSSTIHLFRSSKWTKKSLNNCLSNNRTERGVEFDI 60
           MLTV   TLSPSPFL S QT SHCF+SSTIHLF+ S+  KK LNNCL NNRTERG+EFDI
Sbjct: 1   MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLWANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKGS+RVLDALCGCGIRSLRYLVEAEADF+ ANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 121 NDENRRVIVKNLSRVGRGFGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDVDSFGSDSTF 180
           NDENR VIVKNLSRV  GFG+ RRW+VTNCDANRVM+E YL KDFFDFIDVDSFGSDSTF
Sbjct: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180

Query: 181 LRSAINALKLGGLLYVTSTDGFSSGGHRPSQSLGAYGAYVRPMPFSNEIGLRMLIGGVVR 240
           LR AINALKL GLLYVTSTDGFSSGGH PSQSL AYGAYVRPMPFSNEIGLRMLIGGV R
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKDRHYGFISYCEKCGNSQAFSWSEL 300
           EASVLG+Y TPLFSYYSYHGPVFRVML+I+RG+I K+RHYGF+SYC+KCGNSQ FSWSE+
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEV 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQGKDLEKLLRQ 360
           GQMSCPCND+RVSESLVVSGPLWTGPLHSADYIE+ML+LA QWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360

Query: 361 MVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MVDESD KLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELQQSCLDVHQV 441
           AECIRIALELQQSCL VHQV
Sbjct: 421 AECIRIALELQQSCLGVHQV 440

[Close]
BLAST of Lag0041027 vs. ExPASy TrEMBL
Match: A0A0A0KP99 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G220920 PE=3 SV=1)

HSP 1 Score: 792.7 bits (2046), Expect = 7.7e-226
Identity = 389/440 (88.41%), Postives = 413/440 (93.86%), Query Frame = 0 

Query: 1   MLTVTPKTLSPSPFLHSPQTNSHCFTSSTIHLFRSSKWTKKSLNNCLSNNRTERGVEFDI 60
           MLTV P+TLSPSPFLH PQTNSH  TSS+IHLF+ S   K  LNNCLSNNRTERG++FDI
Sbjct: 1   MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLWANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG +RVLDALCGCGIRSLRYLVE+EADF+ ANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENRRVIVKNLSRVGRGFGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDVDSFGSDSTF 180
           NDENRRVIVKNLSRV    GD+RRW+VTNCDANRVMTE YL KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRSAINALKLGGLLYVTSTDGFSSGGHRPSQSLGAYGAYVRPMPFSNEIGLRMLIGGVVR 240
           LR AINALKL GLLYVTSTDGFSSGGHRPSQSL AYGAYVRPMPFSNEIGLRMLIGGV R
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKDRHYGFISYCEKCGNSQAFSWSEL 300
           EASVLG+YATPLFSYYSYHGPVFRVML+INRG+I+K+RHYGF+SYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQGKDLEKLLRQ 360
           GQMSCPCNDSRVSESLVVSGPLW GPLHSADYIE++L+LAKQWEWIGNGQGKDLEKLLRQ
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 360

Query: 361 MVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MVDESD KLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELQQSCLDVHQV 441
           AECIRIA +LQ++CL  HQV
Sbjct: 421 AECIRIA-QLQRNCLGAHQV 439

[Close]
BLAST of Lag0041027 vs. ExPASy TrEMBL
Match: A0A1S3C509 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103496736 PE=3 SV=1)

HSP 1 Score: 782.3 bits (2019), Expect = 1.0e-222
Identity = 384/440 (87.27%), Postives = 410/440 (93.18%), Query Frame = 0 

Query: 1   MLTVTPKTLSPSPFLHSPQTNSHCFTSSTIHLFRSSKWTKKSLNNCLSNNRTERGVEFDI 60
           MLTV   TLSPSPFL+  QTNSH  TS +IHLF+ S   KKS NNCLSNNRTERG++FDI
Sbjct: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLWANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG +R+LDALCGCGIRSLRYLVE+EADF+ ANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENRRVIVKNLSRVGRGFGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDVDSFGSDSTF 180
           NDENRRVIVKNLS+V   FGD+RRW+VTNCDANRVMTE YL KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRSAINALKLGGLLYVTSTDGFSSGGHRPSQSLGAYGAYVRPMPFSNEIGLRMLIGGVVR 240
           LR AINALKL GLLYVTSTDGFSSGGHRPSQSL AYGAY+RPMPFSNEIGLRMLIGGV R
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKDRHYGFISYCEKCGNSQAFSWSEL 300
           EASVLGYYATPLFSYYSYHGPVFRVML+INRG+I K+RHYGF+SYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQGKDLEKLLRQ 360
           GQMSCPCNDSRVSESLVVSGPLW GPLHSADYIE++L+LAKQWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360

Query: 361 MVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           M+DESD KLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELQQSCLDVHQV 441
           A+CIRIA +LQQ+CL VHQV
Sbjct: 421 ADCIRIA-QLQQNCLGVHQV 439

[Close]
BLAST of Lag0041027 vs. ExPASy TrEMBL
Match: A0A5D3DFE8 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold530G00120 PE=3 SV=1)

HSP 1 Score: 782.3 bits (2019), Expect = 1.0e-222
Identity = 384/440 (87.27%), Postives = 410/440 (93.18%), Query Frame = 0 

Query: 1   MLTVTPKTLSPSPFLHSPQTNSHCFTSSTIHLFRSSKWTKKSLNNCLSNNRTERGVEFDI 60
           MLTV   TLSPSPFL+  QTNSH  TS +IHLF+ S   KKS NNCLSNNRTERG++FDI
Sbjct: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLWANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG +R+LDALCGCGIRSLRYLVE+EADF+ ANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDENRRVIVKNLSRVGRGFGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDVDSFGSDSTF 180
           NDENRRVIVKNLS+V   FGD+RRW+VTNCDANRVMTE YL KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRSAINALKLGGLLYVTSTDGFSSGGHRPSQSLGAYGAYVRPMPFSNEIGLRMLIGGVVR 240
           LR AINALKL GLLYVTSTDGFSSGGHRPSQSL AYGAY+RPMPFSNEIGLRMLIGGV R
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKDRHYGFISYCEKCGNSQAFSWSEL 300
           EASVLGYYATPLFSYYSYHGPVFRVML+INRG+I K+RHYGF+SYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQGKDLEKLLRQ 360
           GQMSCPCNDSRVSESLVVSGPLW GPLHSADYIE++L+LAKQWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360

Query: 361 MVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           M+DESD KLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELQQSCLDVHQV 441
           A+CIRIA +LQQ+CL VHQV
Sbjct: 421 ADCIRIA-QLQQNCLGVHQV 439

[Close]
BLAST of Lag0041027 vs. TAIR 10
Match: AT3G56330.1 (N2,N2-dimethylguanosine tRNA methyltransferase )

HSP 1 Score: 573.5 bits (1477), Expect = 1.4e-163
Identity = 279/433 (64.43%), Postives = 343/433 (79.21%), Query Frame = 0 

Query: 1   MLTVTPKTLSPSPF--LHSPQTNSHCFTSSTIHLFRSSKWTKKSLNNCL--SNNRTERGV 60
           +LT++PKTLS S F  +H  Q N  C +  +       K  K S +  L  S  + ER +
Sbjct: 2   LLTLSPKTLSSSSFFTVHKSQ-NPKCKSPDSCRF----KSLKCSFDRILVKSEVQIERNL 61

Query: 61  EFDIGDTFFRHESATGRDLGVLAAALYRKSKGSIRVLDALCGCGIRSLRYLVEAEADFLW 120
           EF+ G+TFFRHESA GRDLGVL+A LY++S GS+RVLDA+CGCGIRSLRYLVEAEADF+ 
Sbjct: 62  EFETGETFFRHESARGRDLGVLSATLYKRSNGSLRVLDAMCGCGIRSLRYLVEAEADFVM 121

Query: 121 ANDANDENRRVIVKNLSRVGRGFGDDRRWVVTNCDANRVMTEIYLHKDFFDFIDVDSFGS 180
           ANDAND+NRRVI  NLS+V RG GD+RRWVVT+  AN+ M E Y+  DFFD ID+DSFGS
Sbjct: 122 ANDANDDNRRVITDNLSKVERGTGDERRWVVTHMLANKAMIERYMVADFFDMIDIDSFGS 181

Query: 181 DSTFLRSAINALKLGGLLYVTSTDGFSSGGHRPSQSLGAYGAYVRPMPFSNEIGLRMLIG 240
           DS+FLR A NAL+LGGLLY+TSTDG+SSGGHRP  SL AYGA++RPMPF NEIGLRMLIG
Sbjct: 182 DSSFLRDAFNALRLGGLLYLTSTDGYSSGGHRPYNSLAAYGAFIRPMPFGNEIGLRMLIG 241

Query: 241 GVVREASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKDRHYGFISYCEKCGNSQAFS 300
           G VREA++LGY+ TPLFSYYSYHGPVFRVML+++RGK+++DR+YGF+++C  CG+S    
Sbjct: 242 GAVREAALLGYHVTPLFSYYSYHGPVFRVMLRVHRGKLHEDRNYGFVTHCNLCGHSHTLR 301

Query: 301 WSELGQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQGKDLEK 360
           + ELG M CPC+D++ S SLVVSGP+W GPLH A Y+ EML LAK+W W+  G G DL+K
Sbjct: 302 FDELGLMGCPCSDTKASSSLVVSGPMWLGPLHDASYVTEMLELAKEWGWVSEGTGMDLDK 361

Query: 361 LLRQMVDESDPKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKT 420
           LL  M++ESDP+LP GY KMDE+ASRAK+NSPPL  MMS ++K GYAASRSHI  NA+KT
Sbjct: 362 LLSIMIEESDPRLPPGYTKMDEMASRAKMNSPPLKKMMSALVKEGYAASRSHIIPNALKT 421

Query: 421 NCPMAECIRIALE 430
           +CPM+  +RIA E
Sbjct: 422 DCPMSHFVRIAKE 429

[Close]
BLAST of Lag0041027 vs. TAIR 10
Match: AT5G15810.1 (N2,N2-dimethylguanosine tRNA methyltransferase )

HSP 1 Score: 98.2 bits (243), Expect = 1.7e-20
Identity = 94/387 (24.29%), Postives = 161/387 (41.60%), Query Frame = 0 

Query: 59  DIGDTFFRHESATGRDLGVLAAALYRKSKGSI---RVLDALCGCGIRSLRYLVEAEA-DF 118
           D G T   HE  T       A   Y  ++  +   RVL+AL   G+R+LRY  E E    
Sbjct: 186 DNGKTNGEHEVTTQDGPKEAAKTAYESARRELKPPRVLEALSASGLRALRYAREVEGIGQ 245

Query: 119 LWANDANDENRRVIVKNLSRVGRGFGDDRRWVVTNCDANRVMTEIYLH-----KDFFDFI 178
           + A D +  +     +N+   G         +++       +T+  +H     KD FD +
Sbjct: 246 VVALDNDPASVEACQRNIKFNG---------LMSTSKVESHLTDARVHMLSHPKD-FDVV 305

Query: 179 DVDSFGSDSTFLRSAINALKLGGLLYVTSTDGFSSGGHRPSQSLGAYGAYVRPMPFSNEI 238
           D+D +G+ S FL SA+ ++  GGLL  T+TD     G         YG+Y     + +E+
Sbjct: 306 DLDPYGAPSIFLDSAVQSVADGGLLMCTATDMAVLCGANGEVCYSKYGSYPLKGKYCHEM 365

Query: 239 GLRMLIGGVVREASVLGYYATPLFSYYSYHGPVFRVMLQINRGKIYKDRHYGFISYCEKC 298
            LR+L+  +   A+    Y  P+ S         RV +++             +SY  +C
Sbjct: 366 ALRILLASIESHANRYKRYIVPVLSVQMDF--YVRVFVRVYTSASAMKNTPLKLSYVYQC 425

Query: 299 GNSQAFSWSELGQMSCPCNDS----------------RVSESLVVSGPLWTGPLHSADYI 358
               +F    +G+ S P N+S                   +   + GP+W+ P+H  +++
Sbjct: 426 IGCDSFHLQSVGR-SLPKNNSVRYLPGVGPVVPQDCTHCGKKYNMGGPIWSAPIHDQEWV 485

Query: 359 EEMLSLAKQWEWIGNGQGKDLEKLLRQMVDESDPKLPVGYIKMDEVASRAKVNSPPLNTM 418
             +L+  K  +        D    +   + E  P +P+ ++ +  +++  K  SP     
Sbjct: 486 NSILNGVKSMK--DRYPAYDRICAVLTTISEELPDVPL-FLSLHSLSATLKCTSPSAALF 545

Query: 419 MSEMIKAGYAASRSHIASNAIKTNCPM 421
            S +I A Y  S SH+    IKT+ PM
Sbjct: 546 RSAVINAKYRVSGSHVNPLGIKTDAPM 556

[Close]
BLAST of Lag0041027 vs. TAIR 10
Match: AT3G02320.1 (N2,N2-dimethylguanosine tRNA methyltransferase )

HSP 1 Score: 90.9 bits (224), Expect = 2.8e-18
Identity = 81/348 (23.28%), Postives = 145/348 (41.67%), Query Frame = 0 

Query: 91  RVLDALCGCGIRSLRYLVEAEADFLWANDANDENRRVIVKNLSRVGRGFGDDRRWVVTNC 150
           +VL+AL   G+R+LRY  E E         ND      + ++    R    +    ++  
Sbjct: 123 KVLEALSASGLRALRYAREIEGIGQVVALDND------LASVEACQRNIKFNGSVAISKV 182

Query: 151 DANRVMTEIYL--HKDFFDFIDVDSFGSDSTFLRSAINALKLGGLLYVTSTDGFSSGGHR 210
           +++     +++  H   FD +D+D +GS S FL SAI ++  GGLL  T+TD     G  
Sbjct: 183 ESHHTDARVHMLTHPKEFDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDMAVLCGGN 242

Query: 211 PSQSLGAYGAYVRPMPFSNEIGLRMLIGGVVREASVLGYYATPLFSYYSYHGPVFRVMLQ 270
                  YG+Y     + +E+ LR+L+  +   A+    Y  P+ S         RV ++
Sbjct: 243 GEVCYSKYGSYPLRAKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDF--YVRVFVR 302

Query: 271 INRGKIYKDRHYGFISYCEKCGNSQAFSWSELGQMSCPCNDS----------------RV 330
           +             +SY  +C    +F    +G+ S P N+S                  
Sbjct: 303 VYTSASAMKNTPLKLSYVYQCIGCDSFHLQPVGR-SLPKNNSVRYLPAIGPVVKQDCNHC 362

Query: 331 SESLVVSGPLWTGPLHSADYIEEMLSLAKQWEWIGNGQGKDLEKLLRQMVDESDPKLPVG 390
            +   + GP+W+ P+H  +++  +L+  K  +        D    +   V E    +P+ 
Sbjct: 363 GKKYNMGGPIWSAPMHDPEWVTSILNSVKSMK--DRYPAYDRISAVLTTVSEELLDVPL- 422

Query: 391 YIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 421
           ++ +  + +  K  SP      S +I A Y  S +H+    +KT+ PM
Sbjct: 423 FLSLHNLCATLKCISPSAAMFRSAVINANYRISGTHVNPLGMKTDAPM 458

The following BLAST results are available for this feature:
BLAST of Lag0041027 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038892632.14.2e-22688.64tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Benincasa hispida][more]
XP_022943113.15.5e-22688.84tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita moschata][more]
XP_022986521.11.2e-22588.64tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita maxima][more]
XP_011655038.11.6e-22588.41tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus] >XP_011655039.1 tR... [more]
XP_023529370.16.0e-22588.41tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita pepo subsp. pepo][more]
BLAST of Lag0041027 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
B6YUU99.9e-3732.98tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus onnurineus (strain N... [more]
P815541.7e-3330.89tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus furiosus (strain ATCC ... [more]
Q9V1P35.1e-3329.27tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus abyssi (strain GE5 / O... [more]
C5A6N21.8e-3030.59tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus gammatolerans (strai... [more]
Q5JIB34.0e-3030.27tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus kodakarensis (strain... [more]
BLAST of Lag0041027 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1FWD42.6e-22688.84tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1JBD35.9e-22688.64tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A0A0KP997.7e-22688.41tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G... [more]
A0A1S3C5091.0e-22287.27tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103496... [more]
A0A5D3DFE81.0e-22287.27tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=11946... [more]
BLAST of Lag0041027 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G56330.11.4e-16364.43N2,N2-dimethylguanosine tRNA methyltransferase [more]
AT5G15810.11.7e-2024.29N2,N2-dimethylguanosine tRNA methyltransferase [more]
AT3G02320.12.8e-1823.28N2,N2-dimethylguanosine tRNA methyltransferase [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 35..366
e-value: 1.1E-75
score: 256.4
NoneNo IPR availablePANTHERPTHR10631:SF9TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASEcoord: 10..432
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 91..197
e-value: 9.15821E-5
score: 39.7207
IPR042296tRNA methyltransferase, Trm1, C-terminalGENE3D3.30.56.70coord: 372..427
e-value: 3.6E-16
score: 60.6
IPR002905tRNA methyltransferase, Trm1PFAMPF02005TRMcoord: 64..426
e-value: 3.9E-66
score: 223.8
IPR002905tRNA methyltransferase, Trm1PANTHERPTHR10631N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASEcoord: 10..432
IPR002905tRNA methyltransferase, Trm1PROSITEPS51626SAM_MT_TRM1coord: 49..427
score: 98.094246
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 55..427

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0041027.1Lag0041027.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0002940 tRNA N2-guanine methylation
biological_process GO:0008033 tRNA processing
cellular_component GO:0005634 nucleus
molecular_function GO:0000049 tRNA binding
molecular_function GO:0004809 tRNA (guanine-N2-)-methyltransferase activity
molecular_function GO:0003723 RNA binding