Lag0040840 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0040840
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRNase H domain-containing protein
Locationchr13: 8881936 .. 8882552 (+)
RNA-Seq ExpressionLag0040840
SyntenyLag0040840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGATCAACAAGAATTCCTCCAAAAATGACCTTGAATTGGTAGCCACAGCGTGCTGGACTATCTGGAATGACAGAAACAAAGTCATTCATGGAGAAGTTGTTCCACCAACAAGTTTACGAAGCAACTGGATATATAACTACTTGAAGAAATTCCAGAAAGCAAATGTGAAATCGGCTCCGAAAAGAATTTTAAACCCTACCAATGAACAAACAAAAATGAGATCAGGAAGATAAACCTTAAATGACGATTCACTCAAAATTCAGGGATCGGTTTTCTGCGTACGAATTCAGAAGGAGATTTGGTAGGCGCTTCAGCATCGTATGTGAACATGGATTTCAAGGCTTCCCTAGCTGAATTGATAGCAATAAGTGAGGGGTTAAGGCTGGCAAAAATGCTTGGATATGTCAAAATTATGGTTATATCCGACTGTTTACAGGCTATCAAGTTTGTCTCAAAGAATTTTGAACCATGGTGTGAAGTGGAAGCCCAAGTGAAAGAAATTTGGGAGCTGTCAGATCATTGTAATGAATGTAATTTTAGCCATATCTCTAGGGAGAAAAATAAAGCAGCTGACTATATTGCTAAGGAAGCGAAAAGGCTTGAGCTTAATTAA

mRNA sequence

ATGAAGATCAACAAGAATTCCTCCAAAAATGACCTTGAATTGGTAGCCACAGCGTGCTGGACTATCTGGAATGACAGAAACAAAGTCATTCATGGAGAAGTTGTTCCACCAACAAGGATCGGTTTTCTGCGTACGAATTCAGAAGGAGATTTGGTAGGCGCTTCAGCATCGTATGTGAACATGGATTTCAAGGCTTCCCTAGCTGAATTGATAGCAATAAGTGAGGGGTTAAGGCTGGCAAAAATGCTTGGATATGTCAAAATTATGGTTATATCCGACTGTTTACAGGCTATCAAGTTTGTCTCAAAGAATTTTGAACCATGGTGTGAAGTGGAAGCCCAAGTGAAAGAAATTTGGGAGCTGTCAGATCATTGTAATGAATGTAATTTTAGCCATATCTCTAGGGAGAAAAATAAAGCAGCTGACTATATTGCTAAGGAAGCGAAAAGGCTTGAGCTTAATTAA

Coding sequence (CDS)

ATGAAGATCAACAAGAATTCCTCCAAAAATGACCTTGAATTGGTAGCCACAGCGTGCTGGACTATCTGGAATGACAGAAACAAAGTCATTCATGGAGAAGTTGTTCCACCAACAAGGATCGGTTTTCTGCGTACGAATTCAGAAGGAGATTTGGTAGGCGCTTCAGCATCGTATGTGAACATGGATTTCAAGGCTTCCCTAGCTGAATTGATAGCAATAAGTGAGGGGTTAAGGCTGGCAAAAATGCTTGGATATGTCAAAATTATGGTTATATCCGACTGTTTACAGGCTATCAAGTTTGTCTCAAAGAATTTTGAACCATGGTGTGAAGTGGAAGCCCAAGTGAAAGAAATTTGGGAGCTGTCAGATCATTGTAATGAATGTAATTTTAGCCATATCTCTAGGGAGAAAAATAAAGCAGCTGACTATATTGCTAAGGAAGCGAAAAGGCTTGAGCTTAATTAA

Protein sequence

MKINKNSSKNDLELVATACWTIWNDRNKVIHGEVVPPTRIGFLRTNSEGDLVGASASYVNMDFKASLAELIAISEGLRLAKMLGYVKIMVISDCLQAIKFVSKNFEPWCEVEAQVKEIWELSDHCNECNFSHISREKNKAADYIAKEAKRLELN
Homology
BLAST of Lag0040840 vs. NCBI nr
Match: XP_016482447.1 (PREDICTED: uncharacterized protein LOC107803276 [Nicotiana tabacum])

HSP 1 Score: 68.6 bits (166), Expect = 5.5e-08
Identity = 46/139 (33.09%), Postives = 66/139 (47.48%), Query Frame = 0

Query: 20   WTIWNDRNKVIHGEVVPP----------TRIGFLRTNSEGDLVGASASYVNMDFKASLAE 79
            W +W  RN   HGE+V            + IGF+  +S+GD+  A    +        AE
Sbjct: 967  WELWKRRNCQKHGEMVNTDGASRGNPGRSSIGFVLRDSKGDVRYARGKEIKEGTNME-AE 1026

Query: 80   LIAISEGLRLAKMLGYVKIMVISDCLQAIKFVSKNFEPWCEVEAQVKEIWELSDHCNECN 139
             IAI E ++     GY  I + +D L     +   ++P   +EA V+EI +    CN C 
Sbjct: 1027 TIAILEAIQTCVQQGYANIHIQTDSLFLKNVIDGTWDPPWAIEAYVQEIKQFMARCN-CR 1086

Query: 140  FSHISREKNKAADYIAKEA 149
             SHI RE NK AD++A  A
Sbjct: 1087 VSHIMREGNKLADFLANHA 1103

BLAST of Lag0040840 vs. NCBI nr
Match: KAF5202092.1 (Ribonuclease h-like superfamily protein [Thalictrum thalictroides])

HSP 1 Score: 66.2 bits (160), Expect = 2.7e-07
Identity = 44/107 (41.12%), Postives = 63/107 (58.88%), Query Frame = 0

Query: 41  GFLRTNSEGDLVGASASYVNMDFKASL--AELIAISEGLRLAKMLGYVKIMVISDCLQAI 100
           G L  +SEG +V    +Y    F +S+   EL A+ +G++LAK  G+ KI V SD L  I
Sbjct: 297 GGLLRDSEGQVV---CAYSGRGFSSSVYQQELNAVHKGIQLAKDQGFTKIEVASDSLGVI 356

Query: 101 KFVSKNFEPWCEVEAQVKEIWELSDHCNECNFSHISREKNKAADYIA 146
           + ++K  EP  + + QV+EI EL+    E  F H  RE N+AADY+A
Sbjct: 357 RTMNKMEEPPWDCQDQVREIRELAAWFEEIRFYHAFRETNRAADYLA 400

BLAST of Lag0040840 vs. NCBI nr
Match: KAF5186334.1 (Ribonuclease h domain [Thalictrum thalictroides])

HSP 1 Score: 65.5 bits (158), Expect = 4.7e-07
Identity = 38/127 (29.92%), Postives = 63/127 (49.61%), Query Frame = 0

Query: 37  PTRIGFLRTNSEGDLVGASASYVNM------------------DFKASLAELIAISEGLR 96
           P ++GF++ N++G L    A Y  +                  +      EL+AI +G+ 
Sbjct: 220 PPKLGFVKLNTDGSLKKTRAGYGGLLRSNEGRVIECYTAQCKCNKSVLELELLAIQKGIE 279

Query: 97  LAKMLGYVKIMVISDCLQAIKFVSKNFEPWCEVEAQVKEIWELSDHCNECNFSHISREKN 146
           L+   GY ++ V SD L+AI+ + K      +  + V++IWE  +H  E  F+H  RE N
Sbjct: 280 LSIKEGYARLEVASDSLKAIQIIKKKARCPFQCRSTVQDIWEAQNHFEEVTFTHAYRETN 339

BLAST of Lag0040840 vs. NCBI nr
Match: KAF7814237.1 (putative ribonuclease H protein At1g65750 family [Senna tora])

HSP 1 Score: 65.1 bits (157), Expect = 6.1e-07
Identity = 37/110 (33.64%), Postives = 58/110 (52.73%), Query Frame = 0

Query: 37  PTRIGFLRTNSEGDLVGASASYVNMDFKASLAELIAISEGLRLAKMLGYVKIMVISDCLQ 96
           P   G +  N +G  +G  + Y+        AEL AI +GL LA   G+++++V +DCL+
Sbjct: 400 PAGFGGIFRNEDGRWMGGFSGYIGTQTN-MFAELTAIKQGLSLAVNKGFLRLLVETDCLE 459

Query: 97  AIKFVSKNFEPWCEVEAQVKEIWELSDHCNECNFSHISREKNKAADYIAK 147
           A+  +SK       +   + +I  LS  C   +F HI RE N+ AD +AK
Sbjct: 460 ALNLISKGDISTHHLGVIINDIRVLSSRCESISFKHIFREANQCADGLAK 508

BLAST of Lag0040840 vs. NCBI nr
Match: XP_023915006.1 (uncharacterized protein LOC112026546 [Quercus suber])

HSP 1 Score: 63.5 bits (153), Expect = 1.8e-06
Identity = 51/204 (25.00%), Postives = 86/204 (42.16%), Query Frame = 0

Query: 1   MKINKNSSKNDLELVATACWTIWNDRNKVIH-GEVVPPTRI------------------- 60
           + I KN S + LEL     W+IW +RN+ +H G   PP++I                   
Sbjct: 279 LDIIKNDSSSALELFIAVAWSIWWNRNQALHEGSGAPPSQIWDMAIRILGEYKEACSFSN 338

Query: 61  ---------------GFLRTN--------------------SEGDLVGASASYVNMDFKA 120
                          GF + N                    S+G  + AS   ++  F A
Sbjct: 339 LPLVSSLINWRPPPAGFTKINVDGAASDDGRPSSTGVIIRDSQGSSIAASCRVLSSPFPA 398

Query: 121 SLAELIAISEGLRLAKMLGYVKIMVISDCLQAIKFVSKNFEPWCEVEAQVKEIWELSDHC 150
            ++E +A+ +G+ LA  LG  K++  SD L  I+ +++      E+   ++ I +L+   
Sbjct: 399 DISEALALQDGVMLALELGLSKVIFESDALSIIQAINEG-NVGGEIGHIMQNIKDLASSF 458

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_016482447.15.5e-0833.09PREDICTED: uncharacterized protein LOC107803276 [Nicotiana tabacum][more]
KAF5202092.12.7e-0741.12Ribonuclease h-like superfamily protein [Thalictrum thalictroides][more]
KAF5186334.14.7e-0729.92Ribonuclease h domain [Thalictrum thalictroides][more]
KAF7814237.16.1e-0733.64putative ribonuclease H protein At1g65750 family [Senna tora][more]
XP_023915006.11.8e-0625.00uncharacterized protein LOC112026546 [Quercus suber][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 7..149
e-value: 1.7E-14
score: 56.0
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 38..148
e-value: 1.0E-19
score: 70.5
NoneNo IPR availablePANTHERPTHR33033POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 38..150
NoneNo IPR availablePANTHERPTHR33033:SF67SUBFAMILY NOT NAMEDcoord: 38..150
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 41..146
e-value: 1.06656E-16
score: 69.6504
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 48..151

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0040840.1Lag0040840.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity