Lag0040830 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0040830
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	cucumisin-like
Location	chr13: 8785787 .. 8786332 (+)
RNA-Seq Expression	Lag0040830
Synteny	Lag0040830

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATGCTACTGGACTCCATCCAGAAGCAGAGTTTGCATATGGTTCAGGCCATGTCAACCCTCTCAAGGCAGTAAATCCAGGGCTGGTGTACAATGCAACTGAAAGCGACTACGTGAAAGTCTTGTGTGGAATACCAGGTTTCACCACCGCCATGCTCCGGCGAATCACCAGCGACAATAGCGCTTGTACTGGCAGCAACATTGGACGTGACTTGGATCTAAACTATCCTTCTTTCGCTCTCTCAACAGATGGTGGACAATTCTTCAATGAATTTTGCACAAGAACTCTCACAAACGTTGAAACTAAAGTGTCCACATATAGAGCTATAATTTATGTCCCACCAGGCCACAGCATCACGTTCACAGTGAATCCTCCCGTTCAGAAATTCAATGGCATTGGAGATACGAAGTCGTTCACGCTGACTGTTCAAGGATTATTGTCGTCAGGCGACTCAGTTTCTGCTTCTTTGGTGTGGAGTAACGGTGTGCACGAAGTCAGAAGCCCTATAGTACTTTTTCAAGCCGATGATATTGTTAAAGCTTAG

mRNA sequence

ATGGATGCTACTGGACTCCATCCAGAAGCAGAGTTTGCATATGGTTCAGGCCATGTCAACCCTCTCAAGGCAGTAAATCCAGGGCTGGTGTACAATGCAACTGAAAGCGACTACGTGAAAGTCTTGTGTGGAATACCAGGTTTCACCACCGCCATGCTCCGGCGAATCACCAGCGACAATAGCGCTTGTACTGGCAGCAACATTGGACGTGACTTGGATCTAAACTATCCTTCTTTCGCTCTCTCAACAGATGGTGGACAATTCTTCAATGAATTTTGCACAAGAACTCTCACAAACGTTGAAACTAAAGTGTCCACATATAGAGCTATAATTTATGTCCCACCAGGCCACAGCATCACGTTCACAGTGAATCCTCCCGTTCAGAAATTCAATGGCATTGGAGATACGAAGTCGTTCACGCTGACTGTTCAAGGATTATTGTCGTCAGGCGACTCAGTTTCTGCTTCTTTGGTGTGGAGTAACGGTGTGCACGAAGTCAGAAGCCCTATAGTACTTTTTCAAGCCGATGATATTGTTAAAGCTTAG

Coding sequence (CDS)

ATGGATGCTACTGGACTCCATCCAGAAGCAGAGTTTGCATATGGTTCAGGCCATGTCAACCCTCTCAAGGCAGTAAATCCAGGGCTGGTGTACAATGCAACTGAAAGCGACTACGTGAAAGTCTTGTGTGGAATACCAGGTTTCACCACCGCCATGCTCCGGCGAATCACCAGCGACAATAGCGCTTGTACTGGCAGCAACATTGGACGTGACTTGGATCTAAACTATCCTTCTTTCGCTCTCTCAACAGATGGTGGACAATTCTTCAATGAATTTTGCACAAGAACTCTCACAAACGTTGAAACTAAAGTGTCCACATATAGAGCTATAATTTATGTCCCACCAGGCCACAGCATCACGTTCACAGTGAATCCTCCCGTTCAGAAATTCAATGGCATTGGAGATACGAAGTCGTTCACGCTGACTGTTCAAGGATTATTGTCGTCAGGCGACTCAGTTTCTGCTTCTTTGGTGTGGAGTAACGGTGTGCACGAAGTCAGAAGCCCTATAGTACTTTTTCAAGCCGATGATATTGTTAAAGCTTAG

Protein sequence

MDATGLHPEAEFAYGSGHVNPLKAVNPGLVYNATESDYVKVLCGIPGFTTAMLRRITSDNSACTGSNIGRDLDLNYPSFALSTDGGQFFNEFCTRTLTNVETKVSTYRAIIYVPPGHSITFTVNPPVQKFNGIGDTKSFTLTVQGLLSSGDSVSASLVWSNGVHEVRSPIVLFQADDIVKA

Homology

[Close]

BLAST of Lag0040830 vs. NCBI nr
Match: XP_022976634.1 (cucumisin-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 226.9 bits (577), Expect = 1.4e-55
Identity = 116/167 (69.46%), Postives = 130/167 (77.84%), Query Frame = 0


Query: 9   EAEFAYGSGHVNPLKAVNPGLVYNATESDYVKVLCGIPGFTTAMLRRITSDNSACTGSNI 68
           +AEF+YGSG +NPLKAVNPGLVYNA+ESDYV+ LCGIPG T A LR IT ++  CT SN 
Sbjct: 533 QAEFSYGSGQINPLKAVNPGLVYNASESDYVEYLCGIPGITPAQLRLITGEDIICTDSNT 592

Query: 69  GRDLDLNYPSFALSTDGGQFFNEFCTRTLTNVETKVSTYRAIIYVPPGHSITFTVNPPVQ 128
           GRDLDLNYPSFAL    GQ    F  RTLTNVE  VSTYRA +Y PPG ++ F V+PP  
Sbjct: 593 GRDLDLNYPSFALFMGRGQSLRVFFPRTLTNVEATVSTYRATVYSPPGLNVQFKVDPPAL 652

Query: 129 KFNGIGDTKSFTLTVQGLLSSGD--SVSASLVWSNGVHEVRSPIVLF 174
            F+GIGDTKSFTL VQG LS+ D  +VSASLVWSNGV EVRSPIVLF
Sbjct: 653 TFHGIGDTKSFTLGVQGWLSATDPTTVSASLVWSNGVREVRSPIVLF 699

[Close]

BLAST of Lag0040830 vs. NCBI nr
Match: XP_022976643.1 (cucumisin-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 226.9 bits (577), Expect = 1.4e-55
Identity = 116/167 (69.46%), Postives = 130/167 (77.84%), Query Frame = 0


Query: 9   EAEFAYGSGHVNPLKAVNPGLVYNATESDYVKVLCGIPGFTTAMLRRITSDNSACTGSNI 68
           +AEF+YGSG +NPLKAVNPGLVYNA+ESDYV+ LCGIPG T A LR IT ++  CT SN 
Sbjct: 568 QAEFSYGSGQINPLKAVNPGLVYNASESDYVEYLCGIPGITPAQLRLITGEDIICTDSNT 627

Query: 69  GRDLDLNYPSFALSTDGGQFFNEFCTRTLTNVETKVSTYRAIIYVPPGHSITFTVNPPVQ 128
           GRDLDLNYPSFAL    GQ    F  RTLTNVE  VSTYRA +Y PPG ++ F V+PP  
Sbjct: 628 GRDLDLNYPSFALFMGRGQSLRVFFPRTLTNVEATVSTYRATVYSPPGLNVQFKVDPPAL 687

Query: 129 KFNGIGDTKSFTLTVQGLLSSGD--SVSASLVWSNGVHEVRSPIVLF 174
            F+GIGDTKSFTL VQG LS+ D  +VSASLVWSNGV EVRSPIVLF
Sbjct: 688 TFHGIGDTKSFTLGVQGWLSATDPTTVSASLVWSNGVREVRSPIVLF 734

[Close]

BLAST of Lag0040830 vs. NCBI nr
Match: XP_022956553.1 (cucumisin-like [Cucurbita moschata])

HSP 1 Score: 226.5 bits (576), Expect = 1.9e-55
Identity = 116/166 (69.88%), Postives = 130/166 (78.31%), Query Frame = 0


Query: 10  AEFAYGSGHVNPLKAVNPGLVYNATESDYVKVLCGIPGFTTAMLRRITSDNSACTGSNIG 69
           AEFAYGSG +NPLKAVNPGLVYNA+ESDYV+ LCGIP  T A L++IT ++  CT SN G
Sbjct: 570 AEFAYGSGQINPLKAVNPGLVYNASESDYVEYLCGIPNITRAQLQQITGEDIICTDSNTG 629

Query: 70  RDLDLNYPSFALSTDGGQFFNEFCTRTLTNVETKVSTYRAIIYVPPGHSITFTVNPPVQK 129
           RDLDLNYPSFALS   GQ    F  RTLTNVE  VSTYRA +Y PPG ++ F V+PP   
Sbjct: 630 RDLDLNYPSFALSMGRGQSLRVFFPRTLTNVEATVSTYRATVYSPPGLNLQFKVDPPALT 689

Query: 130 FNGIGDTKSFTLTVQGLLSSGD--SVSASLVWSNGVHEVRSPIVLF 174
           F+GIGDTKSFTL VQG LS+ D  +VSASLVWSNGV EVRSPIVLF
Sbjct: 690 FHGIGDTKSFTLGVQGWLSATDPATVSASLVWSNGVREVRSPIVLF 735

[Close]

BLAST of Lag0040830 vs. NCBI nr
Match: XP_023548098.1 (cucumisin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 225.3 bits (573), Expect = 4.2e-55
Identity = 117/175 (66.86%), Postives = 135/175 (77.14%), Query Frame = 0


Query: 1   MDATGLHPEAEFAYGSGHVNPLKAVNPGLVYNATESDYVKVLCGIPGFTTAMLRRITSDN 60
           MDA  ++ +AEF++GSG +NPLKAVNPGLVYNA+ESDYV+ LCGIPG T A LR IT ++
Sbjct: 574 MDAK-VNTQAEFSHGSGQINPLKAVNPGLVYNASESDYVEYLCGIPGITRAQLRLITGED 633

Query: 61  SACTGSNIGRDLDLNYPSFALSTDGGQFFNEFCTRTLTNVETKVSTYRAIIYVPPGHSIT 120
             CT SN GRD+DLNYPSFAL    GQ    F  RTLTNVE  VSTYRA +Y PPG ++ 
Sbjct: 634 IICTDSNTGRDVDLNYPSFALFMGRGQSLRVFFPRTLTNVEATVSTYRATVYSPPGLNVQ 693

Query: 121 FTVNPPVQKFNGIGDTKSFTLTVQGLLSSGD--SVSASLVWSNGVHEVRSPIVLF 174
           F V+PP   F+GIGDTKSFTL VQG LS+ D  +VSASLVWSNGV EVRSPIVLF
Sbjct: 694 FKVDPPALTFHGIGDTKSFTLGVQGWLSATDPTTVSASLVWSNGVREVRSPIVLF 747

[Close]

BLAST of Lag0040830 vs. NCBI nr
Match: KAG6600971.1 (hypothetical protein SDJN03_06204, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 224.6 bits (571), Expect = 7.1e-55
Identity = 115/166 (69.28%), Postives = 129/166 (77.71%), Query Frame = 0


Query: 10  AEFAYGSGHVNPLKAVNPGLVYNATESDYVKVLCGIPGFTTAMLRRITSDNSACTGSNIG 69
           AEFAYGSG +NPLKAVNPGLVYNA+ESDYV+ LCGIPG T A LR IT ++  CT +N G
Sbjct: 255 AEFAYGSGQINPLKAVNPGLVYNASESDYVEYLCGIPGITRAQLRLITGEDIICTDTNTG 314

Query: 70  RDLDLNYPSFALSTDGGQFFNEFCTRTLTNVETKVSTYRAIIYVPPGHSITFTVNPPVQK 129
           RD+DLNYPSFAL    GQ    F  RTLTNVE  VSTYRA +Y PPG ++ F V+PP   
Sbjct: 315 RDVDLNYPSFALFMGRGQSLRVFFPRTLTNVEATVSTYRATVYSPPGLNLQFKVDPPALT 374

Query: 130 FNGIGDTKSFTLTVQGLLSSGD--SVSASLVWSNGVHEVRSPIVLF 174
           F+GIGDTKSFTL VQG LS+ D  +VSASLVWSNGV EVRSPIVLF
Sbjct: 375 FHGIGDTKSFTLGVQGWLSATDPTTVSASLVWSNGVREVRSPIVLF 420

[Close]

BLAST of Lag0040830 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 6.5e-51
Identity = 106/166 (63.86%), Postives = 123/166 (74.10%), Query Frame = 0


Query: 7   HPEAEFAYGSGHVNPLKAVNPGLVYNATESDYVKVLCGIPGFTTAMLRRITSDNSACTGS 66
           +P+AEFAYGSGHVNPLKAV PGLVY+A ESDYVK LCG  G+ T  +RRIT D SACT  
Sbjct: 566 NPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCG-QGYNTQAVRRITGDYSACTSG 625

Query: 67  NIGRDLDLNYPSFALSTDGGQFFNEFCTRTLTNVETKVSTYRAIIYVPPGHSITFTVNPP 126
           N GR  DLNYPSF LS    Q FN++  RTLT+V  + STYRA+I  P G  +T +VNP 
Sbjct: 626 NTGRVWDLNYPSFGLSVSPSQTFNQYFNRTLTSVAPQASTYRAMISAPQG--LTISVNPN 685

Query: 127 VQKFNGIGDTKSFTLTVQGLLSSGDSVSASLVWSNGVHEVRSPIVL 173
           V  FNG+GD KSFTLTV+G +  G  VSASLVWS+GVH VRSPI +
Sbjct: 686 VLSFNGLGDRKSFTLTVRGSI-KGFVVSASLVWSDGVHYVRSPITI 727

[Close]

BLAST of Lag0040830 vs. ExPASy Swiss-Prot
Match: Q9FGU3 (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 2.2e-30
Identity = 71/166 (42.77%), Postives = 105/166 (63.25%), Query Frame = 0


Query: 11  EFAYGSGHVNPLKAVNPGLVYNATESDYVKVLCGIPGFTTAMLRRITSDNSACTGSNIGR 70
           EFAYGSGHV+P+ A+NPGLVY  T++D++  LCG+  +T+  LR I+ DNS CT   I +
Sbjct: 575 EFAYGSGHVDPIDAINPGLVYELTKADHINFLCGL-NYTSDHLRIISGDNSTCT-KEISK 634

Query: 71  DL--DLNYPSFALSTDGGQFFNEFCTRTLTNVETKVSTYRAIIYVPPGHSITFTVNPPVQ 130
            L  +LNYP+ +    G + FN    RT+TNV  + STY A +   PG  ++  V+P V 
Sbjct: 635 TLPRNLNYPTMSAKVSGTKPFNITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVL 694

Query: 131 KFNGIGDTKSFTLTVQG-LLSSGDSVSASLVWSNGVHEVRSPIVLF 174
               + + +SF +TV    + +   VSA+L+WS+G H VRSPI+++
Sbjct: 695 SMKSMNEKQSFMVTVSSDSIGTKQPVSANLIWSDGTHNVRSPIIVY 738

[Close]

BLAST of Lag0040830 vs. ExPASy Swiss-Prot
Match: Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.0e-28
Identity = 73/179 (40.78%), Postives = 105/179 (58.66%), Query Frame = 0


Query: 1   MDATGLH-PEAEFAYGSGHVNPLKAVNPGLVYNATESDYVKVLCGIPGFTTAMLRRITSD 60
           ++ATG      EFAYGSGHV+P+ A NPGLVY   +SD++  LCG+  +T+ +L+ I+ +
Sbjct: 554 VNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGM-NYTSQVLKVISGE 613

Query: 61  NSACTGSNIGRDLDLNYPSFALSTDG-GQFFNEFCTRTLTNVETKVSTYRAIIYVPPGHS 120
              C+ +      +LNYPS +    G G  F     RTLTNV T  STY + +    G  
Sbjct: 614 TVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGTPNSTYTSKVVAGHGSK 673

Query: 121 ITFTVNPPVQKFNGIGDTKSFTLTVQGL-LSSGDSVSASLVWSNGVHEVRSPIVLFQAD 177
           +   + P V  F  + + +SFT+TV G  L S    SA+L+WS+G H VRSPIV++ +D
Sbjct: 674 LDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANLIWSDGTHNVRSPIVVYTSD 731

[Close]

BLAST of Lag0040830 vs. ExPASy Swiss-Prot
Match: Q9FIM6 (Subtilisin-like protease SBT4.8 OS=Arabidopsis thaliana OX=3702 GN=SBT4.8 PE=3 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 5.9e-28
Identity = 71/176 (40.34%), Postives = 108/176 (61.36%), Query Frame = 0


Query: 1   MDATGLHPEA-EFAYGSGHVNPLKAVNPGLVYNATESDYVKVLCGIPGFTTAMLRRITSD 60
           M+ATG   E+ EFAYG+GHV+P+ A+NPGLVY   ++D++  LCG+  +T+  L+ I+ D
Sbjct: 534 MNATGTGAESTEFAYGAGHVDPIAAINPGLVYELNKTDHISFLCGM-NYTSKTLKLISGD 593

Query: 61  NSACTGSNIGRDLDLNYPSF-ALSTDGGQFFNEFCTRTLTNVETKVSTYRAIIYVPPGHS 120
              C+G  + R  +LNYPS  A  ++    F     RT+TN+ T  STY++ I +  G  
Sbjct: 594 AVICSGKTLQR--NLNYPSMSAKLSESNSSFTVTFKRTVTNLGTANSTYKSKIVLNHGSK 653

Query: 121 ITFTVNPPVQKFNGIGDTKSFTLTVQGL-LSSGDSVSASLVWSNGVHEVRSPIVLF 174
           +   V+P V     + + +SFT+TV G  +      SA+L+WS+G H VRSPIV++
Sbjct: 654 LNVKVSPSVLSMKSLKEKQSFTVTVSGSNIDPKLPSSANLIWSDGTHNVRSPIVVY 706

[Close]

BLAST of Lag0040830 vs. ExPASy Swiss-Prot
Match: Q9STF7 (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 1.7e-27
Identity = 71/166 (42.77%), Postives = 100/166 (60.24%), Query Frame = 0


Query: 10  AEFAYGSGHVNPLKAVNPGLVYNATESDYVKVLCGIPGFTTAMLRRITSDNSACTGSNI- 69
           AEFAYG+GHV+P+ A++PGLVY A +SD++  LCG   +T   LR I+ D+S+CT     
Sbjct: 574 AEFAYGAGHVDPIAAIHPGLVYEANKSDHITFLCGF-NYTGKKLRLISGDSSSCTKEQTK 633

Query: 70  GRDLDLNYPSFALSTDGGQFFNEFCTRTLTNVETKVSTYRAIIYVPPGHSITFTVNPPVQ 129
               +LNYPS +    G + F     RT+TNV    +TY+A +    G  +   V P V 
Sbjct: 634 SLTRNLNYPSMSAQVSGTKPFKVTFRRTVTNVGRPNATYKAKVV---GSKLKVKVVPAVL 693

Query: 130 KFNGIGDTKSFTLTVQGLLSSGDS-VSASLVWSNGVHEVRSPIVLF 174
               + + KSFT+TV G     ++ VSA L+WS+GVH VRSPIV++
Sbjct: 694 SLKSLYEKKSFTVTVSGAGPKAENLVSAQLIWSDGVHFVRSPIVVY 735

The following BLAST results are available for this feature:

BLAST of Lag0040830 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022976634.1	1.4e-55	69.46	cucumisin-like isoform X1 [Cucurbita maxima]	[more]
XP_022976643.1	1.4e-55	69.46	cucumisin-like isoform X2 [Cucurbita maxima]	[more]
XP_022956553.1	1.9e-55	69.88	cucumisin-like [Cucurbita moschata]	[more]
XP_023548098.1	4.2e-55	66.86	cucumisin-like [Cucurbita pepo subsp. pepo]	[more]
KAG6600971.1	7.1e-55	69.28	hypothetical protein SDJN03_06204, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Lag0040830 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39547	6.5e-51	63.86	Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1	[more]
Q9FGU3	2.2e-30	42.77	Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 S...	[more]
Q9FIG2	2.0e-28	40.78	Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2...	[more]
Q9FIM6	5.9e-28	40.34	Subtilisin-like protease SBT4.8 OS=Arabidopsis thaliana OX=3702 GN=SBT4.8 PE=3 S...	[more]
Q9STF7	1.7e-27	42.77	Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 S...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 6..38 e-value: 3.8E-8 score: 34.5
None	No IPR available	GENE3D	2.60.40.2310		coord: 42..174 e-value: 1.2E-30 score: 107.9
None	No IPR available	PANTHER	PTHR10795:SF665	SUBTILISIN-LIKE PROTEASE SBT4.1	coord: 7..174
IPR041469	Subtilisin-like protease, fibronectin type-III domain	PFAM	PF17766	fn3_6	coord: 73..171 e-value: 1.1E-20 score: 73.6
IPR045051	Subtilisin-like protease	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 7..174

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0040830.1	Lag0040830.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0016020	membrane
molecular_function	GO:0004252	serine-type endopeptidase activity