Homology
BLAST of Lag0040827 vs. NCBI nr
Match:
XP_023548142.1 (cucumisin-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1210.3 bits (3130), Expect = 0.0e+00
Identity = 609/733 (83.08%), Postives = 658/733 (89.77%), Query Frame = 0
Query: 2 SSLIFKFIFFNLFFSLLVSSSHSDNDARKTYIVYMGSKPEDASSAHLHHRTMLEQVVGST 61
SSL+F FIF NLF LLVSSSHSD+D RKTYIVYMGSKPED SS L+HRTMLEQVVGS+
Sbjct: 8 SSLVFNFIFLNLFSCLLVSSSHSDSDGRKTYIVYMGSKPEDISSTSLYHRTMLEQVVGSS 67
Query: 62 FAPEHLLYSYKRSFNGFAVRLTEEEAQKIALKEGVVSVFPNGKKHLHTTRSWDFMGFTQS 121
F+P+HLLYSYKRSFNGFAVRLT+EEAQKIAL+EGVVSVFPN KKH+HTTRSWDFMGFTQS
Sbjct: 68 FSPKHLLYSYKRSFNGFAVRLTKEEAQKIALEEGVVSVFPNEKKHVHTTRSWDFMGFTQS 127
Query: 122 VARVKQVESNIVVGVLDTGIWPESPSFDDAGLDPPPTKWKGTCQSSTNFQCNRKIIGARI 181
V RV QVESNIVVGVLDTGIWPESPSF+D G+DPPP +WKGTCQ S +FQCNRKIIGAR
Sbjct: 128 VPRVNQVESNIVVGVLDTGIWPESPSFNDEGVDPPPKEWKGTCQISPDFQCNRKIIGARA 187
Query: 182 YRSEEFPPGDIRSPRDSSGHGTHTASTVAGGLVSEASLYGLGSGTARGGVPSARIAVYKI 241
YRSE P G++ +PRDS GHGTHTAS VAGGLV +ASLYGLG GTARGGVPSARIAVYKI
Sbjct: 188 YRSENLPKGEVVNPRDSKGHGTHTASIVAGGLVRKASLYGLGFGTARGGVPSARIAVYKI 247
Query: 242 CWADGCYDADILAAFDDAIADGVDIISLSVGGSAPKPYFNDSIAIGAFHAMKHGILTSNS 301
CW+DGCYDADILAAFDDAIADGVDIISLSVGGS PK YFNDSIAIGAFHAMKHGILTSNS
Sbjct: 248 CWSDGCYDADILAAFDDAIADGVDIISLSVGGSKPKSYFNDSIAIGAFHAMKHGILTSNS 307
Query: 302 AGNNGPRYFTTSNVSPWSLSVAASSMDRKFVTPVHLDNGNSYQGVSINTFDLHGKQYPLI 361
AGN GP YFTTSNVSPWSLSVAAS+MDRKFV+ V NG +QGV+INTFDL KQYP+I
Sbjct: 308 AGNRGPNYFTTSNVSPWSLSVAASTMDRKFVSNVQFGNGTLHQGVAINTFDLLEKQYPMI 367
Query: 362 HARDAPNIAGGFNSSVSRFCVENSVDLNLVRGKIVVCDSLLAPSTVASFNGTVGVIMQGT 421
H DAPN A GFN+S SRFCVENSVDL+LVRGKI+VCDSLL +T+ SF G VG+IMQGT
Sbjct: 368 HGGDAPNKARGFNASTSRFCVENSVDLSLVRGKILVCDSLLRAATMPSFTGAVGIIMQGT 427
Query: 422 GVKDYASSYPLPASYLQFAASNDIQLSSATESSATIFKSIAVQDASAPSVVSFSSRGPNL 481
KDYASSYPLPA+YL AA+NDIQLSS TES+ATI +S AV+DASAPSVVSFSSRGPNL
Sbjct: 428 REKDYASSYPLPATYLHIAAANDIQLSSPTESTATILQSNAVKDASAPSVVSFSSRGPNL 487
Query: 482 VTLDILKPDLTAPGVEILAAWPPIAPVSGVVGDSRSVLYNVISGTSMSCPHVTAIAVYVK 541
VTLDILKPDLTAPGVEILAAW PIAPVSGVVGDSRSV+YN+ISGTSMSCPH TA+AVYVK
Sbjct: 488 VTLDILKPDLTAPGVEILAAWSPIAPVSGVVGDSRSVVYNIISGTSMSCPHATAMAVYVK 547
Query: 542 TFHPTWSPAAIKSALMTTAFTMNANLHPQAEFSYGAGHVNPLKAINPGLVYNASESDYIK 601
TFHPTWSPAAIKSALMTTAFTMN+ L+ QAEF+YGAGHVNPLKAINPGLVYNASESDYI
Sbjct: 548 TFHPTWSPAAIKSALMTTAFTMNSKLNTQAEFAYGAGHVNPLKAINPGLVYNASESDYID 607
Query: 602 FLCGEGYTTEMVRLITGDNSACIGGNIGRVWDLNYPSFALSTTPSQSINQFFTRTLTNVE 661
LCG+GYTT MVRLIT D+SAC N GRVWDLNYPSFALSTTPS+SI+QFFTRTLTNVE
Sbjct: 608 MLCGQGYTTAMVRLITHDSSACTSTNTGRVWDLNYPSFALSTTPSESIHQFFTRTLTNVE 667
Query: 662 SGASIYSATVSAPPSLRIFADPPVLSFNGIGDMKSFRLVVQGTVSQAVVSASLVWSDGVH 721
ASIY+ATV APPSLRI DP VLSFNGIG+ KSFRL VQGTVSQA+VSASLVWSDG+H
Sbjct: 668 LRASIYTATVFAPPSLRITVDPAVLSFNGIGETKSFRLTVQGTVSQAIVSASLVWSDGMH 727
Query: 722 QVRSPITVYVVNK 735
VRSPI VYV+NK
Sbjct: 728 DVRSPIIVYVINK 740
BLAST of Lag0040827 vs. NCBI nr
Match:
XP_038891007.1 (cucumisin-like [Benincasa hispida])
HSP 1 Score: 1192.6 bits (3084), Expect = 0.0e+00
Identity = 611/738 (82.79%), Postives = 654/738 (88.62%), Query Frame = 0
Query: 1 MSSLIFKFIFFNLFFSLLVSSSHSDNDARKTYIVYMGSKPEDASSAHLHHRTMLEQVVGS 60
+SSLI FIF NLF LVSSSHSDND RKTYIVYMG K ED SS LHHRTMLEQVVGS
Sbjct: 6 LSSLILNFIFLNLFNCQLVSSSHSDNDDRKTYIVYMGRKLEDTSSTPLHHRTMLEQVVGS 65
Query: 61 TFAPEHLLYSYKRSFNGFAVRLTEEEAQKIALKEGVVSVFPNGKKHLHTTRSWDFMGFTQ 120
TFAPEHLLYSYKRSFNGFAV+LT+EEAQKIALKEGVVSVFPNGKKH+HTTRSWDFMGFTQ
Sbjct: 66 TFAPEHLLYSYKRSFNGFAVKLTKEEAQKIALKEGVVSVFPNGKKHVHTTRSWDFMGFTQ 125
Query: 121 SVARVKQVESNIVVGVLDTGIWPESPSFDDAGLDPPPTKWKGTCQSSTNFQCNRKIIGAR 180
SV VKQVESNIVVGVLDTGIWPESPSF+DA LDPPP WKG CQ S FQCNRKIIGAR
Sbjct: 126 SVPHVKQVESNIVVGVLDTGIWPESPSFNDADLDPPPAGWKGECQISPTFQCNRKIIGAR 185
Query: 181 IYRSEEFPPGDIRSPRDSSGHGTHTASTVAGGLVSEASLYGLGSGTARGGVPSARIAVYK 240
+YRSE F P DI+SPRDS GHGTHTASTVAGGLVS+ASLYGLG GTARGGVPSARIAVYK
Sbjct: 186 LYRSENFSPEDIQSPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGGVPSARIAVYK 245
Query: 241 ICWADGCYDADILAAFDDAIADGVDIISLSVGGSAPKPYFNDSIAIGAFHAMKHGILTSN 300
ICW+DGCYDADILAAFDDAIADGVDIISLSVGGS PK YFNDSIAIGAFHAM+HGILTSN
Sbjct: 246 ICWSDGCYDADILAAFDDAIADGVDIISLSVGGSEPKSYFNDSIAIGAFHAMQHGILTSN 305
Query: 301 SAGNNGPRYFTTSNVSPWSLSVAASSMDRKFVTPVHLDNGNSYQGVSINTFDLHGKQYPL 360
SAGN GP YFTTSN+SPWSLSVAAS+MDRKFV+ V L NG YQGV+I+TFDL GKQ+PL
Sbjct: 306 SAGNEGPEYFTTSNISPWSLSVAASTMDRKFVSQVQLANGTIYQGVAIHTFDLMGKQHPL 365
Query: 361 IHARDAPNIAGGFNSSVSRFCVENSVDLNLVRGKIVVCDSLLAPSTVASF--NGTVGVIM 420
I+ DAPN AGGFNSS SRFC E SVD +LV+GKI+VCDSLL ST+ SF NG VG+IM
Sbjct: 366 IYGGDAPNKAGGFNSSTSRFCDEKSVDPSLVKGKILVCDSLLRASTMESFNNNGVVGIIM 425
Query: 421 QGTGVKDYASSYPLPASYLQFAASNDIQLSSATESSATIFKSIAVQDASAPSVVSFSSRG 480
QG+ +KDYASSYPLPASYL + DI+LS S+ATIFKS A DASAPSVVSFSSRG
Sbjct: 426 QGSRLKDYASSYPLPASYLH---NEDIKLS----STATIFKSNANLDASAPSVVSFSSRG 485
Query: 481 PNLVTLDILKPDLTAPGVEILAAWPPIAPVSGVVGDSRSVLYNVISGTSMSCPHVTAIAV 540
PNLVTLDILKPDLTAPGVEILAAW PIAPVSG D+RSVLYN+ISGTSMSCPH TAIAV
Sbjct: 486 PNLVTLDILKPDLTAPGVEILAAWSPIAPVSGTARDTRSVLYNIISGTSMSCPHATAIAV 545
Query: 541 YVKTFHPTWSPAAIKSALMTTAFTMNANLHPQAEFSYGAGHVNPLKAINPGLVYNASESD 600
YVKTFHPTWSPAAIKSALMTTAF+MN ++PQAEF+YGAGH+NPLKAINPGLVYNASE+D
Sbjct: 546 YVKTFHPTWSPAAIKSALMTTAFSMNGKVNPQAEFAYGAGHINPLKAINPGLVYNASETD 605
Query: 601 YIKFLCG-EGYTTEMVRLITGDNSACIGGNIGRVWDLNYPSFALSTTPSQSINQFFTRTL 660
YIKFLCG EGYTT+MVR ITGDN+AC N GRVWDLNYPSFALSTTPSQSINQFFTRTL
Sbjct: 606 YIKFLCGHEGYTTDMVRHITGDNTACTPTNSGRVWDLNYPSFALSTTPSQSINQFFTRTL 665
Query: 661 TNVESGASIYSATVSAPPSLRIFADPPVLSFNGIGDMKSFRLVVQGTVSQAVVSASLVWS 720
TNVE S+YSA V APPSLRI +PP+LSFNGIGD KSF+L VQGTVSQA+VSASLVW+
Sbjct: 666 TNVEFRTSLYSAMVFAPPSLRITVEPPLLSFNGIGDTKSFKLTVQGTVSQAIVSASLVWT 725
Query: 721 DGVHQVRSPITVYVVNKA 736
DGVHQVRSPITVYVVNKA
Sbjct: 726 DGVHQVRSPITVYVVNKA 736
BLAST of Lag0040827 vs. NCBI nr
Match:
XP_022139190.1 (LOW QUALITY PROTEIN: cucumisin-like [Momordica charantia])
HSP 1 Score: 1162.1 bits (3005), Expect = 0.0e+00
Identity = 583/736 (79.21%), Postives = 638/736 (86.68%), Query Frame = 0
Query: 1 MSSLIFKFIFFNLFFSLLVSSSHSDNDARKTYIVYMGSKPEDASSAHLHHRTMLEQVVGS 60
MSSLIFKFIFF+LF+ LLVS SH DND RKTYIVYMGSKPED +S LHHR MLE+V+GS
Sbjct: 7 MSSLIFKFIFFSLFYRLLVSGSHIDNDGRKTYIVYMGSKPEDTTSTPLHHRAMLEEVIGS 66
Query: 61 TF---APEHLLYSYKRSFNGFAVRLTEEEAQKIALKEGVVSVFPNGKKHLHTTRSWDFMG 120
TF APE LLYSYKRSFNGFAVRLTEEEA K+A KEGVVSVFPNGKKHLHTTRSWDFMG
Sbjct: 67 TFFLAAPESLLYSYKRSFNGFAVRLTEEEALKVAAKEGVVSVFPNGKKHLHTTRSWDFMG 126
Query: 121 FTQSVARVKQVESNIVVGVLDTGIWPESPSFDDAGLDPPPTKWKGTCQSSTNFQCNRKII 180
F+QSV R QVES++VVGVLDTGIWPESPSF D PPP KW G CQ++ +F+CN+KII
Sbjct: 127 FSQSVPRAHQVESDVVVGVLDTGIWPESPSFIDEAFGPPPAKWMGACQAADDFRCNKKII 186
Query: 181 GARIYRSEEFPPGDIRSPRDSSGHGTHTASTVAGGLVSEASLYGLGSGTARGGVPSARIA 240
GAR+YRSE PP DIRSPRDS+GHGTHTASTVAGGLV +ASLYGLG GTARGGVPSARIA
Sbjct: 187 GARVYRSESLPPSDIRSPRDSNGHGTHTASTVAGGLVEQASLYGLGIGTARGGVPSARIA 246
Query: 241 VYKICWADGCYDADILAAFDDAIADGVDIISLSVGGSAPKPYFNDSIAIGAFHAMKHGIL 300
VYKICW+DGC DADILAAFDDAIADGVDIISLSVGGS P YF+D IAIGAFHAMKHGIL
Sbjct: 247 VYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPNSYFSDPIAIGAFHAMKHGIL 306
Query: 301 TSNSAGNNGPRYFTTSNVSPWSLSVAASSMDRKFVTPVHLDNGNSYQGVSINTFDLHGKQ 360
TSNSAGN GP YFT SN+SPWSLSVAAS+ DRK +T V L NGN YQGV+INTFDL GKQ
Sbjct: 307 TSNSAGNEGPNYFTASNISPWSLSVAASTTDRKILTQVQLGNGNIYQGVAINTFDLLGKQ 366
Query: 361 YPLIHARDAPNIAGGFNSSVSRFCVENSVDLNLVRGKIVVCDSLLAPSTVASFNGTVGVI 420
YPLI+A DAPNIAGGFN S+SR CVENSVD NLVRGKI+VCDSLL S +ASFN TVG++
Sbjct: 367 YPLIYAGDAPNIAGGFNGSISRLCVENSVDKNLVRGKILVCDSLLGASKLASFNATVGIV 426
Query: 421 MQGTGVKDYASSYPLPASYLQFAASNDIQL--SSATESSATIFKSIAVQDASAPSVVSFS 480
MQ +KDYASSYP PASYL+ AA++DIQL SS T+ +ATI KSIAVQDASAP VVSFS
Sbjct: 427 MQDLRLKDYASSYPFPASYLRTAATDDIQLYISSTTDPTATILKSIAVQDASAPVVVSFS 486
Query: 481 SRGPNLVTLDILKPDLTAPGVEILAAWPPIAPVSGVVGDSRSVLYNVISGTSMSCPHVTA 540
SRGP +T DILK DL+APGVEILAAW P+APV GV GDSRS LYN+ISGTSMSCPH TA
Sbjct: 487 SRGPKFITRDILKSDLSAPGVEILAAWSPLAPVLGVKGDSRSELYNIISGTSMSCPHATA 546
Query: 541 IAVYVKTFHPTWSPAAIKSALMTTAFTMNANLHPQAEFSYGAGHVNPLKAINPGLVYNAS 600
IA YVKTFHPTWSPAA+KSALMTTAFTMNA L+PQAEF+YG+GHVNPLKA+NPGLVY+A+
Sbjct: 547 IAAYVKTFHPTWSPAALKSALMTTAFTMNAKLNPQAEFAYGSGHVNPLKAVNPGLVYDAN 606
Query: 601 ESDYIKFLCGEGYTTEMVRLITGDNSACIGGNIGRVWDLNYPSFALSTTPSQSINQFFTR 660
E DY+KFLCG+GY+T MVR ITGD+SAC GNIGRVWDLNYPSFALST PS+ INQFF R
Sbjct: 607 EGDYVKFLCGQGYSTAMVRRITGDSSACTPGNIGRVWDLNYPSFALSTAPSRPINQFFRR 666
Query: 661 TLTNVESGASIYSATVSAPP-SLRIFADPPVLSFNGIGDMKSFRLVVQGTVSQAVVSASL 720
TLTNVESGAS YSA V PP L I +P VLSFNG+GD KSF L VQGTVSQA+VSASL
Sbjct: 667 TLTNVESGASTYSAVVFGPPDGLHITVNPSVLSFNGVGDTKSFNLTVQGTVSQAIVSASL 726
Query: 721 VWSDGVHQVRSPITVY 731
WSDGVHQVRSPIT+Y
Sbjct: 727 EWSDGVHQVRSPITIY 742
BLAST of Lag0040827 vs. NCBI nr
Match:
XP_008461717.1 (PREDICTED: cucumisin-like [Cucumis melo])
HSP 1 Score: 1150.6 bits (2975), Expect = 0.0e+00
Identity = 587/736 (79.76%), Postives = 638/736 (86.68%), Query Frame = 0
Query: 4 LIFKFIFFNLFFSLLVSSSHSDNDARKTYIVYMGSKPEDASSAHLHHRTMLEQVVGSTFA 63
LI F+F NLF V SH DND RKTYIVYMGS ED SS LHHR MLEQV+GS FA
Sbjct: 12 LILNFVFLNLFNCQPVFGSHLDNDGRKTYIVYMGSTLEDTSSTPLHHRAMLEQVIGSNFA 71
Query: 64 PEHLLYSYKRSFNGFAVRLTEEEAQKIALKEGVVSVFPNGKKHLHTTRSWDFMGFTQSVA 123
P+HLLYSYKRSFNGFAVRLTEEEAQKIALKEGVVSVFPNGKKH+HTTRSWDFMGFTQSV
Sbjct: 72 PKHLLYSYKRSFNGFAVRLTEEEAQKIALKEGVVSVFPNGKKHVHTTRSWDFMGFTQSVP 131
Query: 124 RVKQVESNIVVGVLDTGIWPESPSFDDAGLDPPPTKWKGTCQSSTNFQCNRKIIGARIYR 183
RV QVESNIVVGVLDTGIWPESPSF+DA LDPPP WKG CQ+S +FQCNRKIIGAR YR
Sbjct: 132 RVNQVESNIVVGVLDTGIWPESPSFNDADLDPPPAGWKGQCQTSPDFQCNRKIIGARTYR 191
Query: 184 SEEFPPGDIRSPRDSSGHGTHTASTVAGGLVSEASLYGLGSGTARGGVPSARIAVYKICW 243
SE PPG+ +SPRDS GHGTHTASTVAGGLVS+ASLYGLG GTARGGVPSARIAVYKICW
Sbjct: 192 SENLPPGNTQSPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGGVPSARIAVYKICW 251
Query: 244 ADGCYDADILAAFDDAIADGVDIISLSVGGSAPKPYFNDSIAIGAFHAMKHGILTSNSAG 303
+DGCYDADILAAFDDAIADGVDIISLSVGGS K YF DSIAIGAFHAMKHGILTSNSAG
Sbjct: 252 SDGCYDADILAAFDDAIADGVDIISLSVGGSVVKSYFTDSIAIGAFHAMKHGILTSNSAG 311
Query: 304 NNGPRYFTTSNVSPWSLSVAASSMDRKFVTPVHLDNGNSYQGVSINTFDLHGKQYPLIHA 363
N GP YFTTSNVSPWSLSVAAS++DRKFV+ V L NG +YQG +I+TFDL GKQYPLI+
Sbjct: 312 NEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGTAYQGPAIHTFDLMGKQYPLIYG 371
Query: 364 RDAPNIAGGFNSSVSRFCVENSVDLNLVRGKIVVCDSLLAPSTVASF--NGTVGVIMQGT 423
DAPN +GGFNSS+SRFC ENSVD +LV+GKI+VCDS+L ST+ SF NG VG+IMQG+
Sbjct: 372 GDAPNKSGGFNSSISRFCDENSVDQSLVKGKILVCDSILRASTMESFNKNGAVGIIMQGS 431
Query: 424 GVKDYASSYPLPASYLQFAASNDIQLSSATESSATIFKSIAVQDASAPSVVSFSSRGPNL 483
KDYASSYPLPASYL A +I LS S+A+IFKS A+ D+SAPSVVSFSSRGPNL
Sbjct: 432 RFKDYASSYPLPASYLHNA---NITLS----STASIFKSNAILDSSAPSVVSFSSRGPNL 491
Query: 484 VTLDILKPDLTAPGVEILAAWPPIAPVSGVVGDSRSVLYNVISGTSMSCPHVTAIAVYVK 543
TLDILKPDLTAPGVEILAAW PIA VSGV GDSRSVLYN+ISGTSMSCPH TAIAVYVK
Sbjct: 492 ATLDILKPDLTAPGVEILAAWSPIATVSGVAGDSRSVLYNIISGTSMSCPHATAIAVYVK 551
Query: 544 TFHPTWSPAAIKSALMTTAFTMNANLHPQAEFSYGAGHVNPLKAINPGLVYNASESDYIK 603
TF+PTWSPAAIKSALMTTAF+MNA ++PQAEF+YGAGH+NPLKA+NPGLVYNA+E+DYI
Sbjct: 552 TFNPTWSPAAIKSALMTTAFSMNAKVNPQAEFAYGAGHINPLKAVNPGLVYNATETDYIN 611
Query: 604 FLCG-EGYTTEMVRLITGDNSACIGGNIGRVWDLNYPSFALSTTPSQ-SINQFFTRTLTN 663
FLCG EGYTT+MVR ITGDN+AC N GRVWDLNYPSFA STTPSQ +INQFF RTLTN
Sbjct: 612 FLCGQEGYTTDMVRRITGDNTACTPANSGRVWDLNYPSFAFSTTPSQLTINQFFARTLTN 671
Query: 664 VESGASIYSATVSAPPSLRIFADPPVLSFNGIGDMKSFRLVVQGTVSQAVVSASLVWSDG 723
VES S+YSA V APPSLRI DPP L FNGIGD KSF+L VQGTV+Q ++S SLVW+DG
Sbjct: 672 VESKTSLYSAKVFAPPSLRITVDPPSLLFNGIGDTKSFKLTVQGTVNQNIISGSLVWTDG 731
Query: 724 VHQVRSPITVYVVNKA 736
VHQVRSPITVYV KA
Sbjct: 732 VHQVRSPITVYVAKKA 740
BLAST of Lag0040827 vs. NCBI nr
Match:
XP_031741167.1 (cucumisin [Cucumis sativus])
HSP 1 Score: 1124.4 bits (2907), Expect = 0.0e+00
Identity = 573/731 (78.39%), Postives = 626/731 (85.64%), Query Frame = 0
Query: 8 FIFFNLFFSLLVSSSHSDNDARKTYIVYMGSKPEDASSAHLHHRTMLEQVVGSTFAPEHL 67
F++F +FF +TYIVYMGSK ED SS LHHR MLEQVVGS FAP+HL
Sbjct: 11 FLYFYIFF--------------QTYIVYMGSKLEDTSSTPLHHRAMLEQVVGSNFAPKHL 70
Query: 68 LYSYKRSFNGFAVRLTEEEAQKIALKEGVVSVFPNGKKHLHTTRSWDFMGFTQSVARVKQ 127
LYSYKRSFNGFAVRLTEEEAQKIALKEGVVSVFPNGKKH+HTTRSWDFMGFTQSV RV Q
Sbjct: 71 LYSYKRSFNGFAVRLTEEEAQKIALKEGVVSVFPNGKKHVHTTRSWDFMGFTQSVPRVNQ 130
Query: 128 VESNIVVGVLDTGIWPESPSFDDAGLDPPPTKWKGTCQSSTNFQCNRKIIGARIYRSEEF 187
VESNIVVGVLDTGIWPESPSF+D L PPP WKG CQ+S +FQCNRKIIGAR YRSE+
Sbjct: 131 VESNIVVGVLDTGIWPESPSFNDTDLGPPPAGWKGQCQTSPDFQCNRKIIGARTYRSEKL 190
Query: 188 PPGDIRSPRDSSGHGTHTASTVAGGLVSEASLYGLGSGTARGGVPSARIAVYKICWADGC 247
PPG+I+SPRDS GHGTHTASTVAGGLVSEASLYGLG GTARGGVPSARIAVYKICW+DGC
Sbjct: 191 PPGNIQSPRDSEGHGTHTASTVAGGLVSEASLYGLGFGTARGGVPSARIAVYKICWSDGC 250
Query: 248 YDADILAAFDDAIADGVDIISLSVGGSAPKPYFNDSIAIGAFHAMKHGILTSNSAGNNGP 307
YDADILAAFDDAIADGVDIISLSVGGS K YF DSIAIGAFHA+KHGILTSNSAGN GP
Sbjct: 251 YDADILAAFDDAIADGVDIISLSVGGSEVKSYFTDSIAIGAFHAIKHGILTSNSAGNEGP 310
Query: 308 RYFTTSNVSPWSLSVAASSMDRKFVTPVHLDNGNSYQGVSINTFDLHGKQYPLIHARDAP 367
YFTTSNVSPWSLSVAAS++DRKFV+ V L NG YQG +I+TFDL GKQYPLIH DAP
Sbjct: 311 EYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGTVYQGPAIHTFDLMGKQYPLIHGGDAP 370
Query: 368 NIAGGFNSSVSRFCVENSVDLNLVRGKIVVCDSLLAPSTVASF--NGTVGVIMQGTGVKD 427
N +GGFNSS+SR+C ENS+DL+LV+GKI+VCDS+L STV S NG VG+IMQG+ KD
Sbjct: 371 NKSGGFNSSISRYCNENSLDLSLVKGKILVCDSILRASTVESVNKNGAVGIIMQGSRFKD 430
Query: 428 YASSYPLPASYLQFAASNDIQLSSATESSATIFKSIAVQDASAPSVVSFSSRGPNLVTLD 487
YASSYPLPASYL N + S+ATIFKS + +ASAPSVVSFSSRGPNL TLD
Sbjct: 431 YASSYPLPASYLHSTNINTL------SSTATIFKSNEILNASAPSVVSFSSRGPNLATLD 490
Query: 488 ILKPDLTAPGVEILAAWPPIAPVSGVVGDSRSVLYNVISGTSMSCPHVTAIAVYVKTFHP 547
ILKPDLTAPGVEILAAW PIAPVSG+ GDSRSVLYN+ISGTSMSCPH TAIAVYVKTF+P
Sbjct: 491 ILKPDLTAPGVEILAAWSPIAPVSGIAGDSRSVLYNIISGTSMSCPHATAIAVYVKTFNP 550
Query: 548 TWSPAAIKSALMTTAFTMNANLHPQAEFSYGAGHVNPLKAINPGLVYNASESDYIKFLCG 607
TWSPAAIKSALMTTAF+MNA ++P+AEF+YGAGH+NPLKA+NPGLVYNA+E+DYI FLCG
Sbjct: 551 TWSPAAIKSALMTTAFSMNAKVNPEAEFAYGAGHINPLKALNPGLVYNATETDYINFLCG 610
Query: 608 -EGYTTEMVRLITGDNSACIGGNIGRVWDLNYPSFALSTTPSQ-SINQFFTRTLTNVESG 667
EGYTTEMVR ITGD +AC N GRVWDLNYPSFA STTPSQ +INQFFTRTLTNVE
Sbjct: 611 QEGYTTEMVRHITGDKTACTPANSGRVWDLNYPSFAFSTTPSQLTINQFFTRTLTNVEFN 670
Query: 668 ASIYSATVSAPPSLRIFADPPVLSFNGIGDMKSFRLVVQGTVSQAVVSASLVWSDGVHQV 727
S+Y+A V APPSLRI DPP L FNGIGD KSF+L VQGTV+Q +VS SLVW+DGVHQV
Sbjct: 671 TSLYTAKVFAPPSLRITVDPPSLLFNGIGDTKSFKLTVQGTVNQNIVSGSLVWTDGVHQV 721
Query: 728 RSPITVYVVNK 735
RSPITVYVV K
Sbjct: 731 RSPITVYVVKK 721
BLAST of Lag0040827 vs. ExPASy Swiss-Prot
Match:
Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)
HSP 1 Score: 959.1 bits (2478), Expect = 2.8e-278
Identity = 490/736 (66.58%), Postives = 573/736 (77.85%), Query Frame = 0
Query: 2 SSLIFKFIFFNLFFS-LLVSSSHSDNDARKTYIVYMGSKPEDASSAHLHHRTMLEQVVGS 61
SSLIFK FF+LFFS L S SD+D + YIVYMG K ED SAHLHHR MLEQVVGS
Sbjct: 3 SSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVVGS 62
Query: 62 TFAPEHLLYSYKRSFNGFAVRLTEEEAQKIALKEGVVSVFPNGKKHLHTTRSWDFMGFTQ 121
TFAPE +L++YKRSFNGFAV+LTEEEA+KIA EGVVSVF N LHTTRSWDF+GF
Sbjct: 63 TFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFPL 122
Query: 122 SVARVKQVESNIVVGVLDTGIWPESPSFDDAGLDPPPTKWKGTCQSSTNFQCNRKIIGAR 181
+V R QVESNIVVGVLDTGIWPESPSFDD G PPP KWKGTC++S NF+CNRKIIGAR
Sbjct: 123 TVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIGAR 182
Query: 182 IYR-SEEFPPGDIRSPRDSSGHGTHTASTVAGGLVSEASLYGLGSGTARGGVPSARIAVY 241
Y PGD+ PRD++GHGTHTAST AGGLVS+A+LYGLG GTARGGVP ARIA Y
Sbjct: 183 SYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAAY 242
Query: 242 KICWADGCYDADILAAFDDAIADGVDIISLSVGGSAPKPYFNDSIAIGAFHAMKHGILTS 301
K+CW DGC D DILAA+DDAIADGVDIISLSVGG+ P+ YF D+IAIG+FHA++ GILTS
Sbjct: 243 KVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILTS 302
Query: 302 NSAGNNGPRYFTTSNVSPWSLSVAASSMDRKFVTPVHLDNGNSYQGVSINTFDLHGKQYP 361
NSAGN GP +FTT+++SPW LSVAAS+MDRKFVT V + NG S+QGVSINTFD + YP
Sbjct: 303 NSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD--NQYYP 362
Query: 362 LIHARDAPNIAGGFNSSVSRFCVENSVDLNLVRGKIVVCDSLLAP-STVASFNGTVGVIM 421
L+ RD PN GF+ S SRFC + SV+ NL++GKIVVC++ P S +G GV+M
Sbjct: 363 LVSGRDIPNT--GFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGVLM 422
Query: 422 QGTGVKDYASSYPLPASYLQFAASNDI-----QLSSATESSATIFKSIAVQDASAPSVVS 481
+ +DYA SYPLP+S L ND+ + S ATIFKS + +ASAP VVS
Sbjct: 423 T-SNTRDYADSYPLPSSVLD---PNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVS 482
Query: 482 FSSRGPNLVTLDILKPDLTAPGVEILAAWPPIAPVSGVVGDSRSVLYNVISGTSMSCPHV 541
FSSRGPN T D++KPD++ PGVEILAAWP +APV G+ R+ L+N+ISGTSMSCPH+
Sbjct: 483 FSSRGPNRATKDVIKPDISGPGVEILAAWPSVAPVGGI---RRNTLFNIISGTSMSCPHI 542
Query: 542 TAIAVYVKTFHPTWSPAAIKSALMTTAFTMNANLHPQAEFSYGAGHVNPLKAINPGLVYN 601
T IA YVKT++PTWSPAAIKSALMTTA MNA +PQAEF+YG+GHVNPLKA+ PGLVY+
Sbjct: 543 TGIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYD 602
Query: 602 ASESDYIKFLCGEGYTTEMVRLITGDNSACIGGNIGRVWDLNYPSFALSTTPSQSINQFF 661
A+ESDY+KFLCG+GY T+ VR ITGD SAC GN GRVWDLNYPSF LS +PSQ+ NQ+F
Sbjct: 603 ANESDYVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPSFGLSVSPSQTFNQYF 662
Query: 662 TRTLTNVESGASIYSATVSAPPSLRIFADPPVLSFNGIGDMKSFRLVVQGTVSQAVVSAS 721
RTLT+V AS Y A +SAP L I +P VLSFNG+GD KSF L V+G++ VVSAS
Sbjct: 663 NRTLTSVAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSAS 722
Query: 722 LVWSDGVHQVRSPITV 730
LVWSDGVH VRSPIT+
Sbjct: 723 LVWSDGVHYVRSPITI 727
BLAST of Lag0040827 vs. ExPASy Swiss-Prot
Match:
Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)
HSP 1 Score: 639.8 bits (1649), Expect = 3.8e-182
Identity = 345/719 (47.98%), Postives = 457/719 (63.56%), Query Frame = 0
Query: 21 SSHSDNDARKTYIVYMGSKPEDASSAHLHHRTMLEQVVGSTFAPEHLLYSYKRSFNGFAV 80
S++ A YIVYMG+ PE S HH ++L+++VG+ A L+ SYKRSFNGFA
Sbjct: 22 SANDYRQASSVYIVYMGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAA 81
Query: 81 RLTEEEAQKIALKEGVVSVFPNGKKHLHTTRSWDFMGFTQSVARVKQVESNIVVGVLDTG 140
L++ E+QK+ + VVSVFP+ L TTRSWDF+GF + R ES+++VGV+D+G
Sbjct: 82 NLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSG 141
Query: 141 IWPESPSFDDAGLDPPPTKWKGTCQSSTNFQCNRKIIGARIYRSEEFPPGDIRSPRDSSG 200
IWPES SFDD G PPP KWKG+C+ F CN K+IGAR Y S RD G
Sbjct: 142 IWPESESFDDEGFGPPPKKWKGSCKGGLKFACNNKLIGARFYNK------FADSARDEEG 201
Query: 201 HGTHTASTVAGGLVSEASLYGLGSGTARGGVPSARIAVYKICWADGCYDADILAAFDDAI 260
HGTHTAST AG V AS YGL GTARGGVPSARIA YK+C+ + C D DILAAFDDAI
Sbjct: 202 HGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAI 261
Query: 261 ADGVDIISLSVGGSAPKPYFNDSIAIGAFHAMKHGILTSNSAGNNGPRYFTTSNVSPWSL 320
ADGVD+IS+S+ N S+AIG+FHAM GI+T+ SAGNNGP + +NVSPW +
Sbjct: 262 ADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMI 321
Query: 321 SVAASSMDRKFVTPVHLDNGNSYQGVSINTFDLHGKQYPLIHARDAPNIAGGFNSSVSRF 380
+VAAS DR+F+ V L NG + G+S+NTF+L+G ++P+++ + N++ + + + +
Sbjct: 322 TVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVYGQ---NVSRNCSQAQAGY 381
Query: 381 CVENSVDLNLVRGKIVVCDSLLAPSTVASFNGTVGVIMQGTGVKDYASSYPLPASYLQFA 440
C VD LV+GKIV+CD L A G +GVI+Q T + D A P PAS L F
Sbjct: 382 CSSGCVDSELVKGKIVLCDDFLG-YREAYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFE 441
Query: 441 ASNDIQ--LSSATESSATIFKSIAVQDASAPSVVSFSSRGPNLVTLDILKPDLTAPGVEI 500
I+ + SA A I ++ + D AP V SFSSRGP+ V ++LKPD++APG+EI
Sbjct: 442 DYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEI 501
Query: 501 LAAWPPIAPVSGVVG--DSRSVLYNVISGTSMSCPHVTAIAVYVKTFHPTWSPAAIKSAL 560
LAA+ P+A S + D RSV Y+V+SGTSM+CPHV +A YVK+FHP WSP+AIKSA+
Sbjct: 502 LAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAI 561
Query: 561 MTTAFTMNANLHPQAEFSYGAGHVNPLKAINPGLVYNASESDYIKFLCGEGYTTEMVRLI 620
MTTA MN +P+ EF+YG+G +NP KA +PGLVY DY+K LC EG+ + +
Sbjct: 562 MTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTT 621
Query: 621 TGDNSACIGGNIGRVWDLNYPSFALSTTPSQSINQFFTRTLTNVESGASIYSAT-VSAPP 680
+G N C V DLNYP+ + N F RT+TNV S Y A+ V P
Sbjct: 622 SGQNVTC--SERTEVKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQP 681
Query: 681 SLRIFADPPVLSFNGIGDMKSFRLVVQGTVSQ--AVVSASLVWSDGVHQVRSPITVYVV 733
L+I +P +L F + + KSF + + G + + VS+S+VWSDG H VRSPI Y +
Sbjct: 682 ELQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYSI 727
BLAST of Lag0040827 vs. ExPASy Swiss-Prot
Match:
Q9STF7 (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 SV=1)
HSP 1 Score: 612.8 bits (1579), Expect = 4.9e-174
Identity = 347/741 (46.83%), Postives = 467/741 (63.02%), Query Frame = 0
Query: 8 FIFFNLFFSLLV---SSSHSDNDARKTYIVYMGSKPEDASSAHL-HHRTMLEQVVGSTFA 67
+ + F+LLV +S+ D+ ++ YIVYMG+ P + HH ++L+ V G +
Sbjct: 7 YCLLSCIFALLVVSFASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGESSI 66
Query: 68 PEHLLYSYKRSFNGFAVRLTEEEAQKIALKEGVVSVFPNGKKHLHTTRSWDFMGFTQS-- 127
+ L+ +YKRSFNGFA RLTE E + +A + VVSVFP+ +L TT SW+FMG +
Sbjct: 67 QDRLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKR 126
Query: 128 VARVKQVESNIVVGVLDTGIWPESPSFDDAGLDPPPTKWKGTCQSSTNFQCNRKIIGARI 187
R +ES+ ++GV+D+GI+PES SF G PPP KWKG C+ TNF CN K+IGAR
Sbjct: 127 TKRNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIGARY 186
Query: 188 Y--RSEEFPPGDIRSPRDSSGHGTHTASTVAGGLVSEASLYGLGSGTARGGVPSARIAVY 247
Y + E FP S RD++GHG+HTAS AG V S YGLG+GT RGGVP+ARIAVY
Sbjct: 187 YTPKLEGFP----ESARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVY 246
Query: 248 KIC--WADGCYDADILAAFDDAIADGVDIISLSVGGSAPKPYFNDSIAIGAFHAMKHGIL 307
K+C C ILAAFDDAIAD VDII++S+G A + D++AIGAFHAM GIL
Sbjct: 247 KVCDPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGIL 306
Query: 308 TSNSAGNNGPRYFTTSNVSPWSLSVAASSMDRKFVTPVHLDNGNSYQGVSINTFDLHGKQ 367
T N AGNNGP T +++PW +VAAS+M+R F+T V L NG + G S+N+FDL+GK+
Sbjct: 307 TVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKK 366
Query: 368 YPLIHARDAPNIAGGFNSSVSRFCVENSVDLNLVRGKIVVCDSLLAPSTVASFNGTVGVI 427
YPL++ + A + ++S + FC +D V+GKIV+CD+ P + ++
Sbjct: 367 YPLVYGKSA---SSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVASIV 426
Query: 428 MQGTGVKDYASSYPLPASYLQFAASNDI--QLSSATESSATIFKSIAVQDASAPSVVSFS 487
+D AS + P S L N + ++S A + KS + + AP V S+S
Sbjct: 427 R--NPYEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYS 486
Query: 488 SRGPNLVTLDILKPDLTAPGVEILAAWPPIAPVSGVVGDSRSVLYNVISGTSMSCPHVTA 547
SRGPN + DILKPD+TAPG EILAA+ P P S D+R V Y VISGTSMSCPHV
Sbjct: 487 SRGPNPLIHDILKPDITAPGSEILAAYSPYVPPS--ESDTRHVKYTVISGTSMSCPHVAG 546
Query: 548 IAVYVKTFHPTWSPAAIKSALMTTAFTMNANLHPQ---AEFSYGAGHVNPLKAINPGLVY 607
+A Y+KTFHP WSP+ I+SA+MTTA+ MNA+ P AEF+YGAGHV+P+ AI+PGLVY
Sbjct: 547 VAAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVY 606
Query: 608 NASESDYIKFLCGEGYTTEMVRLITGDNSACIGGNIGRVW-DLNYPSFALSTTPSQSINQ 667
A++SD+I FLCG YT + +RLI+GD+S+C + +LNYPS + + ++
Sbjct: 607 EANKSDHITFLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKPFKV 666
Query: 668 FFTRTLTNVESGASIYSATVSAPPSLRIFADPPVLSFNGIGDMKSFRLVVQGTVSQA--V 727
F RT+TNV + Y A V L++ P VLS + + KSF + V G +A +
Sbjct: 667 TFRRTVTNVGRPNATYKAKV-VGSKLKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAENL 726
Query: 728 VSASLVWSDGVHQVRSPITVY 731
VSA L+WSDGVH VRSPI VY
Sbjct: 727 VSAQLIWSDGVHFVRSPIVVY 735
BLAST of Lag0040827 vs. ExPASy Swiss-Prot
Match:
F4JA91 (Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana OX=3702 GN=SBT4.5 PE=3 SV=1)
HSP 1 Score: 612.5 bits (1578), Expect = 6.4e-174
Identity = 350/751 (46.60%), Postives = 468/751 (62.32%), Query Frame = 0
Query: 1 MSSLIFKFIFFNLFFSLLV---SSSHSDNDARKTYIVYMGSKPEDASSAHL-HHRTMLEQ 60
M+ + + F+LLV +S+ D+ ++ YIVYMG+ P + HH ++L+
Sbjct: 1 MTKPAVSYCLLSCIFALLVVSFASADKDDQDKQEYIVYMGALPARVDYMPMSHHTSILQD 60
Query: 61 VVGSTFAPEHLLYSYKRSFNGFAVRLTEEEAQKIALKEGVVSVFPNGKKHLHTTRSWDFM 120
V G + + L+ +YKRSFNGFA RLT+ E + +A + VVSVFPN K L TT SW+FM
Sbjct: 61 VTGESSIEDRLVRNYKRSFNGFAARLTKSEREILASMDEVVSVFPNKKLKLQTTTSWNFM 120
Query: 121 GFTQS--VARVKQVESNIVVGVLDTGIWPESPSFDDAGLDPPPTKWKGTCQSSTNFQCNR 180
G +S R +ES+ ++GV+D+GI+PES SF G PPP KWKG C+ NF N
Sbjct: 121 GLKESKRTKRNTIIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGKNFTWNN 180
Query: 181 KIIGARIY--RSEEFPPGDIRSPRDSSGHGTHTASTVAGGLVSEASLYGLGSGTARGGVP 240
K+IGAR Y + E FP S RD GHG+HTAST AG V S YGLG+GTARGGVP
Sbjct: 181 KLIGARYYTPKLEGFP----ESARDYMGHGSHTASTAAGNAVKHVSFYGLGNGTARGGVP 240
Query: 241 SARIAVYKIC--WADGCYDADILAAFDDAIADGVDIISLSVGGSAPKPYFNDSIAIGAFH 300
+ARIAVYK+C DGC ILAAFDDAIAD VDII++S+GG P+ D IAIGAFH
Sbjct: 241 AARIAVYKVCDPGVDGCTTDGILAAFDDAIADKVDIITISIGGDNSSPFEEDPIAIGAFH 300
Query: 301 AMKHGILTSNSAGNNGPRYFTTSNVSPWSLSVAASSMDRKFVTPVHLDNGNSYQGVSINT 360
AM GIL NSAGN+GP T ++++PW +VAAS+ +R FVT V L NG + G S+N+
Sbjct: 301 AMAKGILIVNSAGNSGPEPSTVASIAPWMFTVAASNTNRAFVTKVVLGNGKTV-GRSVNS 360
Query: 361 FDLHGKQYPLIHARDAPNIAGGFNSSVSRFCVENSVDLNLVRGKIVVCDSLLAPSTVASF 420
FDL+GK+YPL++ + A + G ++ FC +D V+GKIV+CDS P +
Sbjct: 361 FDLNGKKYPLVYGKSASSSCGAASAG---FCSPGCLDSKRVKGKIVLCDSPQNPDEAQAM 420
Query: 421 NGTVGVIMQGTGVKDYASSYPLPASYLQFAASNDI--QLSSATESSATIFKSIAVQDASA 480
++ D AS + P S L N + ++S A + KS + + A
Sbjct: 421 GAIASIVRSHR--TDVASIFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKSETIFNQRA 480
Query: 481 PSVVSFSSRGPNLVTLDILKPDLTAPGVEILAAWPPIAPVSGVVGDSRSVLYNVISGTSM 540
P V S+ SRGPN + DILKPD+TAPG EI+AA+ P AP S + D+R V Y+V +GTSM
Sbjct: 481 PVVASYFSRGPNTIIPDILKPDITAPGSEIVAAYSPDAPPS--ISDTRRVKYSVDTGTSM 540
Query: 541 SCPHVTAIAVYVKTFHPTWSPAAIKSALMTTAFTMNANLHP---QAEFSYGAGHVNPLKA 600
SCPHV +A Y+K+FHP WSP+ I+SA+MTTA+ MNA+ P AEF+YGAGHV+P+ A
Sbjct: 541 SCPHVAGVAAYLKSFHPRWSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDPITA 600
Query: 601 INPGLVYNASESDYIKFLCGEGYTTEMVRLITGDNSACIGGNIGRV-WDLNYPSFALSTT 660
I+PGLVY A++SD+I FLCG YT + +RLI+GD+S+C + +LNYPS +
Sbjct: 601 IHPGLVYEANKSDHIAFLCGLNYTAKNLRLISGDSSSCTKEQTKSLPRNLNYPSMTAQVS 660
Query: 661 PSQSINQFFTRTLTNVESGASIYSATVSAPPSLRIFADPPVLSFNGIGDMKSFRLVVQGT 720
++ F RT+TNV + Y A V L++ P VLS + + KSF + G
Sbjct: 661 AAKPFKVIFRRTVTNVGRPNATYKAKV-VGSKLKVKVVPAVLSLKSLYEKKSFTVTASGA 720
Query: 721 VSQA--VVSASLVWSDGVHQVRSPITVYVVN 734
+A +VSA L+WSDGVH VRSPI VY N
Sbjct: 721 GPKAENLVSAQLIWSDGVHFVRSPIVVYATN 738
BLAST of Lag0040827 vs. ExPASy Swiss-Prot
Match:
Q9LZS6 (Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana OX=3702 GN=SBT4.15 PE=3 SV=1)
HSP 1 Score: 611.3 bits (1575), Expect = 1.4e-173
Identity = 353/752 (46.94%), Postives = 481/752 (63.96%), Query Frame = 0
Query: 11 FNLFFSLLVSSSHSDNDARKTYIVYMGSKPEDA--SSAHLHHRTMLEQVVGSTFAPEHLL 70
F L + +++++ +N RK YIVYMG E++ +A HH ++ + + A E +
Sbjct: 14 FCLVNNAVIAATEDENVERKPYIVYMGEATENSLVEAAENHHNLLMTVIGDESKARELKI 73
Query: 71 YSYKRSFNGFAVRLTEEEAQKIALKEGVVSVFPNGKKHLHTTRSWDFMGFTQS-VARVKQ 130
YSY ++ NGF RL EA+K++ +EGVVSVF N ++ LHTTRSWDF+G +S R
Sbjct: 74 YSYGKNINGFVARLFPHEAEKLSREEGVVSVFKNTQRQLHTTRSWDFLGLVESKYKRSVG 133
Query: 131 VESNIVVGVLDTGIWPESPSFDDAGLDPPPTKWKGTCQSSTNF-QCNRKIIGARIY--RS 190
+ESNI+VGVLDTGI ESPSF+D G+ PPP KWKG C + NF +CN K+IGA+ + +S
Sbjct: 134 IESNIIVGVLDTGIDVESPSFNDKGVGPPPAKWKGKCVTGNNFTRCNNKVIGAKYFHIQS 193
Query: 191 EEFPPGDIRSPRDSSGHGTHTASTVAGGLVSEASLYGLGSGTARGGVPSARIAVYKICWA 250
E P G+ + D GHGTHT+ST+AG VS ASL+G+ +GTARGGVPSARIA YK+CW
Sbjct: 194 EGLPDGEGDTAADHDGHGTHTSSTIAGVSVSSASLFGIANGTARGGVPSARIAAYKVCWD 253
Query: 251 DGCYDADILAAFDDAIADGVDIISLSVGGSAPKPYFNDSIAIGAFHAMKHGILTSNSAGN 310
GC D D+LAAFD+AI+DGVDIIS+S+GG A P+F D IAIGAFHAMK GILT+ SAGN
Sbjct: 254 SGCTDMDMLAAFDEAISDGVDIISISIGG-ASLPFFEDPIAIGAFHAMKRGILTTCSAGN 313
Query: 311 NGPRYFTTSNVSPWSLSVAASSMDRKFVTPVHLDNGNSYQGVSINTFDLHGKQYPLIHAR 370
NGP FT SN++PW ++VAA+S+DRKF T V L NG + G+S+N F+ K YPL
Sbjct: 314 NGPGLFTVSNLAPWVMTVAANSLDRKFETVVKLGNGLTASGISLNGFNPRKKMYPLTSGS 373
Query: 371 DAPNI-AGGFNSSVSRFCVENSVDLNLVRGKIVVCDSLLAPS---------TVASFNGTV 430
A N+ AGG+ + C ++ + V GK+V C++ V S G
Sbjct: 374 LASNLSAGGYGEPST--CEPGTLGEDKVMGKVVYCEAGREEGGNGGQGQDHVVRSLKG-A 433
Query: 431 GVIMQGTGVKDYASSYPLPASYLQFAASNDI--QLSSATESSATIFKSIAVQDASAPSVV 490
GVI+Q D A+S + SY+ F I ++S A IFK+ + APS+
Sbjct: 434 GVIVQLLEPTDMATSTLIAGSYVFFEDGTKITEYINSTKNPQAVIFKTKTTK-MLAPSIS 493
Query: 491 SFSSRGPNLVTLDILKPDLTAPGVEILAAWPPIAPVSGVVGDSRSVLYNVISGTSMSCPH 550
SFS+RGP ++ +ILKPD++APG+ ILAA+ +A V+G D+R L++++SGTSM+CPH
Sbjct: 494 SFSARGPQRISPNILKPDISAPGLNILAAYSKLASVTGYPDDNRRTLFSIMSGTSMACPH 553
Query: 551 VTAIAVYVKTFHPTWSPAAIKSALMTTAFTMNANLHPQAEFSYGAGHVNPLKAINPGLVY 610
A A YVK+FHP WSPAAIKSALMTTA M + +AE SYG+G +NP +AI+PGLVY
Sbjct: 554 AAAAAAYVKSFHPDWSPAAIKSALMTTATPMRIKGN-EAELSYGSGQINPRRAIHPGLVY 613
Query: 611 NASESDYIKFLCGEGYTTEMVRLITGDNS--------ACIGGNIGRVWD-LNYPSFALST 670
+ +E Y++FLC EGY + + L+TGDNS C G D LNYPS
Sbjct: 614 DITEDAYLRFLCKEGYNSTSIGLLTGDNSNNTTKKEYNCENIKRGLGSDGLNYPSLHKQV 673
Query: 671 TPSQS-INQFFTRTLTNVESGASIYSATVSAPPSLRIFADPPVLSFNGIGDMKSFRLVVQ 730
+++ +++ F RT+TNV G S Y A V AP LR+ P V+SF + ++F++V+
Sbjct: 674 NSTEAKVSEVFYRTVTNVGYGPSTYVARVWAPKGLRVEVVPKVMSFERPKEKRNFKVVID 733
BLAST of Lag0040827 vs. ExPASy TrEMBL
Match:
A0A6J1CBL9 (LOW QUALITY PROTEIN: cucumisin-like OS=Momordica charantia OX=3673 GN=LOC111010158 PE=3 SV=1)
HSP 1 Score: 1162.1 bits (3005), Expect = 0.0e+00
Identity = 583/736 (79.21%), Postives = 638/736 (86.68%), Query Frame = 0
Query: 1 MSSLIFKFIFFNLFFSLLVSSSHSDNDARKTYIVYMGSKPEDASSAHLHHRTMLEQVVGS 60
MSSLIFKFIFF+LF+ LLVS SH DND RKTYIVYMGSKPED +S LHHR MLE+V+GS
Sbjct: 7 MSSLIFKFIFFSLFYRLLVSGSHIDNDGRKTYIVYMGSKPEDTTSTPLHHRAMLEEVIGS 66
Query: 61 TF---APEHLLYSYKRSFNGFAVRLTEEEAQKIALKEGVVSVFPNGKKHLHTTRSWDFMG 120
TF APE LLYSYKRSFNGFAVRLTEEEA K+A KEGVVSVFPNGKKHLHTTRSWDFMG
Sbjct: 67 TFFLAAPESLLYSYKRSFNGFAVRLTEEEALKVAAKEGVVSVFPNGKKHLHTTRSWDFMG 126
Query: 121 FTQSVARVKQVESNIVVGVLDTGIWPESPSFDDAGLDPPPTKWKGTCQSSTNFQCNRKII 180
F+QSV R QVES++VVGVLDTGIWPESPSF D PPP KW G CQ++ +F+CN+KII
Sbjct: 127 FSQSVPRAHQVESDVVVGVLDTGIWPESPSFIDEAFGPPPAKWMGACQAADDFRCNKKII 186
Query: 181 GARIYRSEEFPPGDIRSPRDSSGHGTHTASTVAGGLVSEASLYGLGSGTARGGVPSARIA 240
GAR+YRSE PP DIRSPRDS+GHGTHTASTVAGGLV +ASLYGLG GTARGGVPSARIA
Sbjct: 187 GARVYRSESLPPSDIRSPRDSNGHGTHTASTVAGGLVEQASLYGLGIGTARGGVPSARIA 246
Query: 241 VYKICWADGCYDADILAAFDDAIADGVDIISLSVGGSAPKPYFNDSIAIGAFHAMKHGIL 300
VYKICW+DGC DADILAAFDDAIADGVDIISLSVGGS P YF+D IAIGAFHAMKHGIL
Sbjct: 247 VYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPNSYFSDPIAIGAFHAMKHGIL 306
Query: 301 TSNSAGNNGPRYFTTSNVSPWSLSVAASSMDRKFVTPVHLDNGNSYQGVSINTFDLHGKQ 360
TSNSAGN GP YFT SN+SPWSLSVAAS+ DRK +T V L NGN YQGV+INTFDL GKQ
Sbjct: 307 TSNSAGNEGPNYFTASNISPWSLSVAASTTDRKILTQVQLGNGNIYQGVAINTFDLLGKQ 366
Query: 361 YPLIHARDAPNIAGGFNSSVSRFCVENSVDLNLVRGKIVVCDSLLAPSTVASFNGTVGVI 420
YPLI+A DAPNIAGGFN S+SR CVENSVD NLVRGKI+VCDSLL S +ASFN TVG++
Sbjct: 367 YPLIYAGDAPNIAGGFNGSISRLCVENSVDKNLVRGKILVCDSLLGASKLASFNATVGIV 426
Query: 421 MQGTGVKDYASSYPLPASYLQFAASNDIQL--SSATESSATIFKSIAVQDASAPSVVSFS 480
MQ +KDYASSYP PASYL+ AA++DIQL SS T+ +ATI KSIAVQDASAP VVSFS
Sbjct: 427 MQDLRLKDYASSYPFPASYLRTAATDDIQLYISSTTDPTATILKSIAVQDASAPVVVSFS 486
Query: 481 SRGPNLVTLDILKPDLTAPGVEILAAWPPIAPVSGVVGDSRSVLYNVISGTSMSCPHVTA 540
SRGP +T DILK DL+APGVEILAAW P+APV GV GDSRS LYN+ISGTSMSCPH TA
Sbjct: 487 SRGPKFITRDILKSDLSAPGVEILAAWSPLAPVLGVKGDSRSELYNIISGTSMSCPHATA 546
Query: 541 IAVYVKTFHPTWSPAAIKSALMTTAFTMNANLHPQAEFSYGAGHVNPLKAINPGLVYNAS 600
IA YVKTFHPTWSPAA+KSALMTTAFTMNA L+PQAEF+YG+GHVNPLKA+NPGLVY+A+
Sbjct: 547 IAAYVKTFHPTWSPAALKSALMTTAFTMNAKLNPQAEFAYGSGHVNPLKAVNPGLVYDAN 606
Query: 601 ESDYIKFLCGEGYTTEMVRLITGDNSACIGGNIGRVWDLNYPSFALSTTPSQSINQFFTR 660
E DY+KFLCG+GY+T MVR ITGD+SAC GNIGRVWDLNYPSFALST PS+ INQFF R
Sbjct: 607 EGDYVKFLCGQGYSTAMVRRITGDSSACTPGNIGRVWDLNYPSFALSTAPSRPINQFFRR 666
Query: 661 TLTNVESGASIYSATVSAPP-SLRIFADPPVLSFNGIGDMKSFRLVVQGTVSQAVVSASL 720
TLTNVESGAS YSA V PP L I +P VLSFNG+GD KSF L VQGTVSQA+VSASL
Sbjct: 667 TLTNVESGASTYSAVVFGPPDGLHITVNPSVLSFNGVGDTKSFNLTVQGTVSQAIVSASL 726
Query: 721 VWSDGVHQVRSPITVY 731
WSDGVHQVRSPIT+Y
Sbjct: 727 EWSDGVHQVRSPITIY 742
BLAST of Lag0040827 vs. ExPASy TrEMBL
Match:
A0A1S3CF95 (cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500249 PE=3 SV=1)
HSP 1 Score: 1150.6 bits (2975), Expect = 0.0e+00
Identity = 587/736 (79.76%), Postives = 638/736 (86.68%), Query Frame = 0
Query: 4 LIFKFIFFNLFFSLLVSSSHSDNDARKTYIVYMGSKPEDASSAHLHHRTMLEQVVGSTFA 63
LI F+F NLF V SH DND RKTYIVYMGS ED SS LHHR MLEQV+GS FA
Sbjct: 12 LILNFVFLNLFNCQPVFGSHLDNDGRKTYIVYMGSTLEDTSSTPLHHRAMLEQVIGSNFA 71
Query: 64 PEHLLYSYKRSFNGFAVRLTEEEAQKIALKEGVVSVFPNGKKHLHTTRSWDFMGFTQSVA 123
P+HLLYSYKRSFNGFAVRLTEEEAQKIALKEGVVSVFPNGKKH+HTTRSWDFMGFTQSV
Sbjct: 72 PKHLLYSYKRSFNGFAVRLTEEEAQKIALKEGVVSVFPNGKKHVHTTRSWDFMGFTQSVP 131
Query: 124 RVKQVESNIVVGVLDTGIWPESPSFDDAGLDPPPTKWKGTCQSSTNFQCNRKIIGARIYR 183
RV QVESNIVVGVLDTGIWPESPSF+DA LDPPP WKG CQ+S +FQCNRKIIGAR YR
Sbjct: 132 RVNQVESNIVVGVLDTGIWPESPSFNDADLDPPPAGWKGQCQTSPDFQCNRKIIGARTYR 191
Query: 184 SEEFPPGDIRSPRDSSGHGTHTASTVAGGLVSEASLYGLGSGTARGGVPSARIAVYKICW 243
SE PPG+ +SPRDS GHGTHTASTVAGGLVS+ASLYGLG GTARGGVPSARIAVYKICW
Sbjct: 192 SENLPPGNTQSPRDSEGHGTHTASTVAGGLVSQASLYGLGFGTARGGVPSARIAVYKICW 251
Query: 244 ADGCYDADILAAFDDAIADGVDIISLSVGGSAPKPYFNDSIAIGAFHAMKHGILTSNSAG 303
+DGCYDADILAAFDDAIADGVDIISLSVGGS K YF DSIAIGAFHAMKHGILTSNSAG
Sbjct: 252 SDGCYDADILAAFDDAIADGVDIISLSVGGSVVKSYFTDSIAIGAFHAMKHGILTSNSAG 311
Query: 304 NNGPRYFTTSNVSPWSLSVAASSMDRKFVTPVHLDNGNSYQGVSINTFDLHGKQYPLIHA 363
N GP YFTTSNVSPWSLSVAAS++DRKFV+ V L NG +YQG +I+TFDL GKQYPLI+
Sbjct: 312 NEGPEYFTTSNVSPWSLSVAASTIDRKFVSRVQLANGTAYQGPAIHTFDLMGKQYPLIYG 371
Query: 364 RDAPNIAGGFNSSVSRFCVENSVDLNLVRGKIVVCDSLLAPSTVASF--NGTVGVIMQGT 423
DAPN +GGFNSS+SRFC ENSVD +LV+GKI+VCDS+L ST+ SF NG VG+IMQG+
Sbjct: 372 GDAPNKSGGFNSSISRFCDENSVDQSLVKGKILVCDSILRASTMESFNKNGAVGIIMQGS 431
Query: 424 GVKDYASSYPLPASYLQFAASNDIQLSSATESSATIFKSIAVQDASAPSVVSFSSRGPNL 483
KDYASSYPLPASYL A +I LS S+A+IFKS A+ D+SAPSVVSFSSRGPNL
Sbjct: 432 RFKDYASSYPLPASYLHNA---NITLS----STASIFKSNAILDSSAPSVVSFSSRGPNL 491
Query: 484 VTLDILKPDLTAPGVEILAAWPPIAPVSGVVGDSRSVLYNVISGTSMSCPHVTAIAVYVK 543
TLDILKPDLTAPGVEILAAW PIA VSGV GDSRSVLYN+ISGTSMSCPH TAIAVYVK
Sbjct: 492 ATLDILKPDLTAPGVEILAAWSPIATVSGVAGDSRSVLYNIISGTSMSCPHATAIAVYVK 551
Query: 544 TFHPTWSPAAIKSALMTTAFTMNANLHPQAEFSYGAGHVNPLKAINPGLVYNASESDYIK 603
TF+PTWSPAAIKSALMTTAF+MNA ++PQAEF+YGAGH+NPLKA+NPGLVYNA+E+DYI
Sbjct: 552 TFNPTWSPAAIKSALMTTAFSMNAKVNPQAEFAYGAGHINPLKAVNPGLVYNATETDYIN 611
Query: 604 FLCG-EGYTTEMVRLITGDNSACIGGNIGRVWDLNYPSFALSTTPSQ-SINQFFTRTLTN 663
FLCG EGYTT+MVR ITGDN+AC N GRVWDLNYPSFA STTPSQ +INQFF RTLTN
Sbjct: 612 FLCGQEGYTTDMVRRITGDNTACTPANSGRVWDLNYPSFAFSTTPSQLTINQFFARTLTN 671
Query: 664 VESGASIYSATVSAPPSLRIFADPPVLSFNGIGDMKSFRLVVQGTVSQAVVSASLVWSDG 723
VES S+YSA V APPSLRI DPP L FNGIGD KSF+L VQGTV+Q ++S SLVW+DG
Sbjct: 672 VESKTSLYSAKVFAPPSLRITVDPPSLLFNGIGDTKSFKLTVQGTVNQNIISGSLVWTDG 731
Query: 724 VHQVRSPITVYVVNKA 736
VHQVRSPITVYV KA
Sbjct: 732 VHQVRSPITVYVAKKA 740
BLAST of Lag0040827 vs. ExPASy TrEMBL
Match:
A0A6J1CEK6 (cucumisin-like OS=Momordica charantia OX=3673 GN=LOC111010021 PE=3 SV=1)
HSP 1 Score: 1097.8 bits (2838), Expect = 0.0e+00
Identity = 560/738 (75.88%), Postives = 620/738 (84.01%), Query Frame = 0
Query: 2 SSLIFKFIFFNLFFSLLVSSSHSDN-DARKTYIVYMGSKPEDASSAHLHHRTMLEQVVGS 61
SSLIFK +F +L LL SS S+N D RK YIVY+G+KPED +S HH MLE+VVGS
Sbjct: 3 SSLIFKLVFLSLLCGLLASSLDSNNDDGRKIYIVYLGNKPEDTASTPSHHMRMLEEVVGS 62
Query: 62 TFAPEHLLYSYKRSFNGFAVRLTEEEAQKIALKEGVVSVFPNGKKHLHTTRSWDFMGFTQ 121
TFAPE LL+SYKRSFNGF V+LTEEEAQKIA KEGVVSVF NGKKHLHTTRSWDFMGFT+
Sbjct: 63 TFAPEALLHSYKRSFNGFVVKLTEEEAQKIAAKEGVVSVFLNGKKHLHTTRSWDFMGFTK 122
Query: 122 SVARVKQVESNIVVGVLDTGIWPESPSFDDAGLDPPPTKWKGTCQSSTNFQCNRKIIGAR 181
+VARVKQVESNIVVGVLD+GIWPESPSF D G PPP KWKGTCQ+S NF CN+KIIGAR
Sbjct: 123 NVARVKQVESNIVVGVLDSGIWPESPSFSDLGYGPPPVKWKGTCQTSANFHCNKKIIGAR 182
Query: 182 IYRSEE-FPPGDIRSPRDSSGHGTHTASTVAGGLVSEASLYGLGSGTARGGVPSARIAVY 241
YRS FPP DIRSPRDS GHGTHTASTVAGGLV++ASLYGL GTARGGVPSARIAVY
Sbjct: 183 AYRSNNFFPPEDIRSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAVY 242
Query: 242 KICWADGCYDADILAAFDDAIADGVDIISLSVGGSAPKPYFNDSIAIGAFHAMKHGILTS 301
KICW+DGCYDADILAAFDDAIADGVDIISLSVGGS PK YFNDSIAIGAFH+MKHGILTS
Sbjct: 243 KICWSDGCYDADILAAFDDAIADGVDIISLSVGGSEPKYYFNDSIAIGAFHSMKHGILTS 302
Query: 302 NSAGNNGPRYFTTSNVSPWSLSVAASSMDRKFVTPVHLDNGNSYQGVSINTFDLHGKQYP 361
NSAGN+GP YFT N SPWSLSVAASS+DRKFVT V L N N YQG +INTFDL GKQYP
Sbjct: 303 NSAGNDGPDYFTIRNFSPWSLSVAASSIDRKFVTKVQLGNKNLYQGYTINTFDLLGKQYP 362
Query: 362 LIHARDAPNIAGGFNSSVSRFCVENSVDLNLVRGKIVVCDSLLAPSTVASFNGTVGVIMQ 421
LI+ DAPNI GGF SS SRFC +N+VD NLV+GKI+VCD++L+PS ASF G VGV+MQ
Sbjct: 363 LIYGGDAPNITGGFTSSSSRFCSKNTVDRNLVKGKILVCDTVLSPSAFASFXGAVGVVMQ 422
Query: 422 GTGVKDYASSYPLPASYLQFAASNDIQ--LSSATESSATIFKSIAVQDASAPSVVSFSSR 481
GVKD A SYPLPASYL AA I+ ++S ++ATI KS AV D SAP VVSFSSR
Sbjct: 423 DAGVKDNARSYPLPASYLGTAAGGSIKNYMASNRAATATILKSNAVNDTSAPLVVSFSSR 482
Query: 482 GPNLVTLDILKPDLTAPGVEILAAWPPIAPVSGVVGDSRSVLYNVISGTSMSCPHVTAIA 541
GPN T DILKPDLTAPGVEILAAW PIAP+SG+ DSR LYN+ISGTSMSCPH TA+A
Sbjct: 483 GPNPETFDILKPDLTAPGVEILAAWSPIAPISGIT-DSRKSLYNIISGTSMSCPHATAVA 542
Query: 542 VYVKTFHPTWSPAAIKSALMTTAFTMNANLHPQAEFSYGAGHVNPLKAINPGLVYNASES 601
VYVKTFHPTWSPAAIKSALMTTA +N L+PQAEF+YGAGHVNPLKA+NPGLVY+A ES
Sbjct: 543 VYVKTFHPTWSPAAIKSALMTTATPLNPKLNPQAEFAYGAGHVNPLKAVNPGLVYDAHES 602
Query: 602 DYIKFLCGEGYTTEMVRLITGDNSACIGGNIGRVWDLNYPSFALSTTPSQSINQFFTRTL 661
DY+KFLCG+GYTT MV+ +T D SAC N RVWDLNYPSFALS+TPS+SINQFFTRTL
Sbjct: 603 DYVKFLCGQGYTTAMVQRLTADASACTPANTARVWDLNYPSFALSSTPSESINQFFTRTL 662
Query: 662 TNVESGASIYSATV-SAPPSLRIFADPPVLSFNGIGDMKSFRLVVQGTVSQAVVSASLVW 721
TNV S AS Y A V APP L I PPVLSF+ IG+ KSF+L ++GT+S ++VSASLVW
Sbjct: 663 TNVGSKASTYKAAVYGAPPGLTISVSPPVLSFHAIGEKKSFKLTIRGTLSHSIVSASLVW 722
Query: 722 SDGVHQVRSPITVYVVNK 735
+DG+HQVRSPITVYVVNK
Sbjct: 723 NDGLHQVRSPITVYVVNK 739
BLAST of Lag0040827 vs. ExPASy TrEMBL
Match:
A0A6J1K719 (cucumisin-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490766 PE=3 SV=1)
HSP 1 Score: 1075.5 bits (2780), Expect = 0.0e+00
Identity = 538/738 (72.90%), Postives = 611/738 (82.79%), Query Frame = 0
Query: 2 SSLIFKFIFFNLFFSLLVSSSHSDNDARKTYIVYMGSKPEDASSAHLHHRTMLEQVVGST 61
SS+IFK +F F LL SS SDND RK YIVY+G+KP+D +S HH +LE+VVGST
Sbjct: 15 SSVIFKLVFLGFFGCLLASSLDSDNDGRKVYIVYLGNKPDDMASTPSHHMRLLEEVVGST 74
Query: 62 FAPEHLLYSYKRSFNGFAVRLTEEEAQKIALKEGVVSVFPNGKKHLHTTRSWDFMGFTQS 121
F+P+ LL+SYKRSFNGFAVRLTEEEAQKI K+GVVSVFPNGKKHLHTTRSWDFMGFT +
Sbjct: 75 FSPDALLHSYKRSFNGFAVRLTEEEAQKIRAKDGVVSVFPNGKKHLHTTRSWDFMGFTTN 134
Query: 122 VARVKQVESNIVVGVLDTGIWPESPSFDDAGLDPPPTKWKGTCQSSTNFQCNRKIIGARI 181
V RV QVES+IVVGVLD+GIWPESPSF D G P P KWKG CQ+S NF+CN+KIIGAR
Sbjct: 135 VPRVNQVESDIVVGVLDSGIWPESPSFSDVGYGPLPAKWKGACQASNNFRCNKKIIGARA 194
Query: 182 YRSE-EFPPGDIRSPRDSSGHGTHTASTVAGGLVSEASLYGLGSGTARGGVPSARIAVYK 241
YRS+ FPP D SPRDS GHGTHTASTVAGGLVS+ASLYGL GTARGGVPSARIAVYK
Sbjct: 195 YRSDNNFPPEDFESPRDSDGHGTHTASTVAGGLVSQASLYGLALGTARGGVPSARIAVYK 254
Query: 242 ICWADGCYDADILAAFDDAIADGVDIISLSVGGSAPKPYFNDSIAIGAFHAMKHGILTSN 301
ICW+DGCYDADILAAFDDAIADGVDIISLSVGGS PK YFNDSIAIGAFH+MKHGILTSN
Sbjct: 255 ICWSDGCYDADILAAFDDAIADGVDIISLSVGGSEPKYYFNDSIAIGAFHSMKHGILTSN 314
Query: 302 SAGNNGPRYFTTSNVSPWSLSVAASSMDRKFVTPVHLDNGNSYQGVSINTFDLHGKQYPL 361
SAGN+GP YFT N SPWSLSVAASS+DRK V+ V L N N +QG +INTFDL GK YPL
Sbjct: 315 SAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSDVQLGNKNIFQGYTINTFDLQGKHYPL 374
Query: 362 IHARDAPNIAGGFNSSVSRFCVENSVDLNLVRGKIVVCDSLLAPSTVASFNGTVGVIMQG 421
I+A APNI+GGF+ S SR+C +NSVD NLV+GKI+VCDS+L+PST AS + VGV+M
Sbjct: 375 IYAGHAPNISGGFSGSSSRYCSKNSVDRNLVKGKILVCDSILSPSTFASLSSAVGVVMNN 434
Query: 422 TGVKDYASSYPLPASYLQFAASNDIQ--LSSATESSATIFKSIAVQDASAPSVVSFSSRG 481
GVKD A SYPLP+SYL AA N ++ + S +ATIFKS V D +AP VVSFSSRG
Sbjct: 435 AGVKDNARSYPLPSSYLTRAAGNHVKNYIGSNNFPTATIFKSNEVNDTAAPIVVSFSSRG 494
Query: 482 PNLVTLDILKPDLTAPGVEILAAWPPIAPVSGVVGDSRSVLYNVISGTSMSCPHVTAIAV 541
PN T DILKPDLTAPGVEILAAW PIAPVS + DSR+ LYN+ISGTSMSCPH TA AV
Sbjct: 495 PNPETYDILKPDLTAPGVEILAAWAPIAPVSSGIKDSRTTLYNIISGTSMSCPHATAAAV 554
Query: 542 YVKTFHPTWSPAAIKSALMTTAFTMNANLHPQAEFSYGAGHVNPLKAINPGLVYNASESD 601
YVKTFHPTWSPAAIKSALMTTA +N L+PQAEF+YGAGH++PLKA+NPGL+Y+A+E+D
Sbjct: 555 YVKTFHPTWSPAAIKSALMTTAIPLNVELNPQAEFAYGAGHIDPLKAMNPGLIYDANETD 614
Query: 602 YIKFLCGEGYTTEMVRLITGDNSACIGGNIGRVWDLNYPSFALSTTPSQSINQFFTRTLT 661
Y+ FLCG+GY+T MVR +TGD S C N GRVWDLNYPSFALSTTPS+SINQFFTRTLT
Sbjct: 615 YVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQFFTRTLT 674
Query: 662 NVESGASIYSATV-SAPPSLRIFADPPVLSFNGIGDMKSFRLVVQGTVSQAVVSASLVWS 721
NV S AS Y++ + AP L I DPPVLSFNGIG +SF L + GT+SQ++VSAS+VWS
Sbjct: 675 NVGSEASTYTSKILGAPEGLTITVDPPVLSFNGIGHKESFTLTIGGTISQSIVSASVVWS 734
Query: 722 DGVHQVRSPITVYVVNKA 736
DG H VRSPIT+Y+VNKA
Sbjct: 735 DGSHNVRSPITIYIVNKA 752
BLAST of Lag0040827 vs. ExPASy TrEMBL
Match:
A0A6J1IQ27 (cucumisin-like OS=Cucurbita maxima OX=3661 GN=LOC111478984 PE=3 SV=1)
HSP 1 Score: 1074.3 bits (2777), Expect = 2.7e-310
Identity = 543/740 (73.38%), Postives = 615/740 (83.11%), Query Frame = 0
Query: 2 SSLIFKFIFFNLFFSLLVSSS--HSDNDARKTYIVYMGSKPEDASSAHLHHRTMLEQVVG 61
SSLIFK + F F L++SS SDND RK YIVY+G+KPED++S HH MLE+VVG
Sbjct: 3 SSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEVVG 62
Query: 62 STFAPEHLLYSYKRSFNGFAVRLTEEEAQKIALKEGVVSVFPNGKKHLHTTRSWDFMGFT 121
STFAP+ LL+SYKRSFNGF V+LTEEEAQKI+ KEGVVSVFPNGKKHLHTTRSWDFMGFT
Sbjct: 63 STFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMGFT 122
Query: 122 QSVARVKQVESNIVVGVLDTGIWPESPSFDDAGLDPPPTKWKGTCQSSTNFQCNRKIIGA 181
+SV RVKQVESNIVVGVLD+GIWPESPSF D G PPP KWKG CQ+S NF+CNRKIIGA
Sbjct: 123 KSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKIIGA 182
Query: 182 RIYRSEE-FPPGDIRSPRDSSGHGTHTASTVAGGLVSEASLYGLGSGTARGGVPSARIAV 241
R YRS+ FPP DI+SPRDS GHGTHTASTVAGGLV++ASLYGL GTARGGVPSARIAV
Sbjct: 183 RAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARIAV 242
Query: 242 YKICWADGCYDADILAAFDDAIADGVDIISLSVGGSAPKPYFNDSIAIGAFHAMKHGILT 301
YKICW+DGCYDADILAAFDDAIADGVDIISLSVGG+ PK YFNDSIAIGAFH+MKHGILT
Sbjct: 243 YKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGILT 302
Query: 302 SNSAGNNGPRYFTTSNVSPWSLSVAASSMDRKFVTPVHLDNGNSYQGVSINTFDLHGKQY 361
SNSAGN+GP YFT N SPWSLSVAASS+DRK V+ V L N N YQG +INTFDL GKQY
Sbjct: 303 SNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGKQY 362
Query: 362 PLIHARDAPNIAGGFNSSVSRFCVENSVDLNLVRGKIVVCDSLLAPSTVASFNGTVGVIM 421
PLI+A +APN++GGF S SRFC NSVD NLVRGKI++CDS+L+PST ASFNG VGV+M
Sbjct: 363 PLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGVVM 422
Query: 422 QGTGVKDYASSYPLPASYLQFAASNDIQ--LSSATESSATIFKSIAVQDASAPSVVSFSS 481
GVKD + SYPLP+SYL A N+I+ + S +ATIFKS AV D SAP +VSFSS
Sbjct: 423 NDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSFSS 482
Query: 482 RGPNLVTLDILKPDLTAPGVEILAAWPPIAPVSGVVGDSRSVLYNVISGTSMSCPHVTAI 541
RGPN T DILKPDLTAPGVEILAAW PIA VS V DSR+ LYN+ISGTSMSCPH TA
Sbjct: 483 RGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHATAA 542
Query: 542 AVYVKTFHPTWSPAAIKSALMTTAFTMNANLHPQAEFSYGAGHVNPLKAINPGLVYNASE 601
AVYVKTFHP+WSPAAIKSALMTTA +N L+PQAEF+YGAGH+NP+KA+NPGLVY+A E
Sbjct: 543 AVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDAQE 602
Query: 602 SDYIKFLCGEGYTTEMVRLITGDNSACIGGNIGRVWDLNYPSFALSTTPSQSINQFFTRT 661
SDY++FLCG+GYTT MVR ++GD+S C N GRVWDLNYPSFALS+TPS+SINQFF RT
Sbjct: 603 SDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFRRT 662
Query: 662 LTNVESGASIYSATV-SAPPSLRIFADPPVLSFNGIGDMKSFRLVVQGTVSQAVVSASLV 721
+TNV S + Y A V AP L I +PP LSFN IG KSF + V+G V+Q +VSA+L+
Sbjct: 663 VTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAALL 722
Query: 722 WSDGVHQVRSPITVYVVNKA 736
W+DG H VRSPITVYVV+KA
Sbjct: 723 WTDGHHHVRSPITVYVVDKA 742
BLAST of Lag0040827 vs. TAIR 10
Match:
AT5G59190.1 (subtilase family protein )
HSP 1 Score: 628.6 bits (1620), Expect = 6.2e-180
Identity = 339/704 (48.15%), Postives = 449/704 (63.78%), Query Frame = 0
Query: 36 MGSKPEDASSAHLHHRTMLEQVVGSTFAPEHLLYSYKRSFNGFAVRLTEEEAQKIALKEG 95
MG+ PE S HH ++L+++VG+ A L+ SYKRSFNGFA L++ E+QK+ +
Sbjct: 1 MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 60
Query: 96 VVSVFPNGKKHLHTTRSWDFMGFTQSVARVKQVESNIVVGVLDTGIWPESPSFDDAGLDP 155
VVSVFP+ L TTRSWDF+GF + R ES+++VGV+D+GIWPES SFDD G P
Sbjct: 61 VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGP 120
Query: 156 PPTKWKGTCQSSTNFQCNRKIIGARIYRSEEFPPGDIRSPRDSSGHGTHTASTVAGGLVS 215
PP KWKG+C+ F CN K+IGAR Y S RD GHGTHTAST AG V
Sbjct: 121 PPKKWKGSCKGGLKFACNNKLIGARFYNK------FADSARDEEGHGTHTASTAAGNAVQ 180
Query: 216 EASLYGLGSGTARGGVPSARIAVYKICWADGCYDADILAAFDDAIADGVDIISLSVGGSA 275
AS YGL GTARGGVPSARIA YK+C+ + C D DILAAFDDAIADGVD+IS+S+
Sbjct: 181 AASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISISADY 240
Query: 276 PKPYFNDSIAIGAFHAMKHGILTSNSAGNNGPRYFTTSNVSPWSLSVAASSMDRKFVTPV 335
N S+AIG+FHAM GI+T+ SAGNNGP + +NVSPW ++VAAS DR+F+ V
Sbjct: 241 VSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRV 300
Query: 336 HLDNGNSYQGVSINTFDLHGKQYPLIHARDAPNIAGGFNSSVSRFCVENSVDLNLVRGKI 395
L NG + G+S+NTF+L+G ++P+++ + N++ + + + +C VD LV+GKI
Sbjct: 301 VLGNGKALTGISVNTFNLNGTKFPIVYGQ---NVSRNCSQAQAGYCSSGCVDSELVKGKI 360
Query: 396 VVCDSLLAPSTVASFNGTVGVIMQGTGVKDYASSYPLPASYLQFAASNDIQ--LSSATES 455
V+CD L A G +GVI+Q T + D A P PAS L F I+ + SA
Sbjct: 361 VLCDDFLG-YREAYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPP 420
Query: 456 SATIFKSIAVQDASAPSVVSFSSRGPNLVTLDILKPDLTAPGVEILAAWPPIAPVSGVVG 515
A I ++ + D AP V SFSSRGP+ V ++LKPD++APG+EILAA+ P+A S +
Sbjct: 421 QAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLN 480
Query: 516 --DSRSVLYNVISGTSMSCPHVTAIAVYVKTFHPTWSPAAIKSALMTTAFTMNANLHPQA 575
D RSV Y+V+SGTSM+CPHV +A YVK+FHP WSP+AIKSA+MTTA MN +P+
Sbjct: 481 PEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQ 540
Query: 576 EFSYGAGHVNPLKAINPGLVYNASESDYIKFLCGEGYTTEMVRLITGDNSACIGGNIGRV 635
EF+YG+G +NP KA +PGLVY DY+K LC EG+ + + +G N C V
Sbjct: 541 EFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTC--SERTEV 600
Query: 636 WDLNYPSFALSTTPSQSINQFFTRTLTNVESGASIYSAT-VSAPPSLRIFADPPVLSFNG 695
DLNYP+ + N F RT+TNV S Y A+ V P L+I +P +L F
Sbjct: 601 KDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGF 660
Query: 696 IGDMKSFRLVVQGTVSQ--AVVSASLVWSDGVHQVRSPITVYVV 733
+ + KSF + + G + + VS+S+VWSDG H VRSPI Y +
Sbjct: 661 LEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYSI 691
BLAST of Lag0040827 vs. TAIR 10
Match:
AT3G46850.1 (Subtilase family protein )
HSP 1 Score: 612.8 bits (1579), Expect = 3.5e-175
Identity = 347/741 (46.83%), Postives = 467/741 (63.02%), Query Frame = 0
Query: 8 FIFFNLFFSLLV---SSSHSDNDARKTYIVYMGSKPEDASSAHL-HHRTMLEQVVGSTFA 67
+ + F+LLV +S+ D+ ++ YIVYMG+ P + HH ++L+ V G +
Sbjct: 7 YCLLSCIFALLVVSFASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGESSI 66
Query: 68 PEHLLYSYKRSFNGFAVRLTEEEAQKIALKEGVVSVFPNGKKHLHTTRSWDFMGFTQS-- 127
+ L+ +YKRSFNGFA RLTE E + +A + VVSVFP+ +L TT SW+FMG +
Sbjct: 67 QDRLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKR 126
Query: 128 VARVKQVESNIVVGVLDTGIWPESPSFDDAGLDPPPTKWKGTCQSSTNFQCNRKIIGARI 187
R +ES+ ++GV+D+GI+PES SF G PPP KWKG C+ TNF CN K+IGAR
Sbjct: 127 TKRNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIGARY 186
Query: 188 Y--RSEEFPPGDIRSPRDSSGHGTHTASTVAGGLVSEASLYGLGSGTARGGVPSARIAVY 247
Y + E FP S RD++GHG+HTAS AG V S YGLG+GT RGGVP+ARIAVY
Sbjct: 187 YTPKLEGFP----ESARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVY 246
Query: 248 KIC--WADGCYDADILAAFDDAIADGVDIISLSVGGSAPKPYFNDSIAIGAFHAMKHGIL 307
K+C C ILAAFDDAIAD VDII++S+G A + D++AIGAFHAM GIL
Sbjct: 247 KVCDPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGIL 306
Query: 308 TSNSAGNNGPRYFTTSNVSPWSLSVAASSMDRKFVTPVHLDNGNSYQGVSINTFDLHGKQ 367
T N AGNNGP T +++PW +VAAS+M+R F+T V L NG + G S+N+FDL+GK+
Sbjct: 307 TVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKK 366
Query: 368 YPLIHARDAPNIAGGFNSSVSRFCVENSVDLNLVRGKIVVCDSLLAPSTVASFNGTVGVI 427
YPL++ + A + ++S + FC +D V+GKIV+CD+ P + ++
Sbjct: 367 YPLVYGKSA---SSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVASIV 426
Query: 428 MQGTGVKDYASSYPLPASYLQFAASNDI--QLSSATESSATIFKSIAVQDASAPSVVSFS 487
+D AS + P S L N + ++S A + KS + + AP V S+S
Sbjct: 427 R--NPYEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYS 486
Query: 488 SRGPNLVTLDILKPDLTAPGVEILAAWPPIAPVSGVVGDSRSVLYNVISGTSMSCPHVTA 547
SRGPN + DILKPD+TAPG EILAA+ P P S D+R V Y VISGTSMSCPHV
Sbjct: 487 SRGPNPLIHDILKPDITAPGSEILAAYSPYVPPS--ESDTRHVKYTVISGTSMSCPHVAG 546
Query: 548 IAVYVKTFHPTWSPAAIKSALMTTAFTMNANLHPQ---AEFSYGAGHVNPLKAINPGLVY 607
+A Y+KTFHP WSP+ I+SA+MTTA+ MNA+ P AEF+YGAGHV+P+ AI+PGLVY
Sbjct: 547 VAAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVY 606
Query: 608 NASESDYIKFLCGEGYTTEMVRLITGDNSACIGGNIGRVW-DLNYPSFALSTTPSQSINQ 667
A++SD+I FLCG YT + +RLI+GD+S+C + +LNYPS + + ++
Sbjct: 607 EANKSDHITFLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKPFKV 666
Query: 668 FFTRTLTNVESGASIYSATVSAPPSLRIFADPPVLSFNGIGDMKSFRLVVQGTVSQA--V 727
F RT+TNV + Y A V L++ P VLS + + KSF + V G +A +
Sbjct: 667 TFRRTVTNVGRPNATYKAKV-VGSKLKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAENL 726
Query: 728 VSASLVWSDGVHQVRSPITVY 731
VSA L+WSDGVH VRSPI VY
Sbjct: 727 VSAQLIWSDGVHFVRSPIVVY 735
BLAST of Lag0040827 vs. TAIR 10
Match:
AT3G46840.1 (Subtilase family protein )
HSP 1 Score: 612.5 bits (1578), Expect = 4.6e-175
Identity = 350/751 (46.60%), Postives = 468/751 (62.32%), Query Frame = 0
Query: 1 MSSLIFKFIFFNLFFSLLV---SSSHSDNDARKTYIVYMGSKPEDASSAHL-HHRTMLEQ 60
M+ + + F+LLV +S+ D+ ++ YIVYMG+ P + HH ++L+
Sbjct: 1 MTKPAVSYCLLSCIFALLVVSFASADKDDQDKQEYIVYMGALPARVDYMPMSHHTSILQD 60
Query: 61 VVGSTFAPEHLLYSYKRSFNGFAVRLTEEEAQKIALKEGVVSVFPNGKKHLHTTRSWDFM 120
V G + + L+ +YKRSFNGFA RLT+ E + +A + VVSVFPN K L TT SW+FM
Sbjct: 61 VTGESSIEDRLVRNYKRSFNGFAARLTKSEREILASMDEVVSVFPNKKLKLQTTTSWNFM 120
Query: 121 GFTQS--VARVKQVESNIVVGVLDTGIWPESPSFDDAGLDPPPTKWKGTCQSSTNFQCNR 180
G +S R +ES+ ++GV+D+GI+PES SF G PPP KWKG C+ NF N
Sbjct: 121 GLKESKRTKRNTIIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGKNFTWNN 180
Query: 181 KIIGARIY--RSEEFPPGDIRSPRDSSGHGTHTASTVAGGLVSEASLYGLGSGTARGGVP 240
K+IGAR Y + E FP S RD GHG+HTAST AG V S YGLG+GTARGGVP
Sbjct: 181 KLIGARYYTPKLEGFP----ESARDYMGHGSHTASTAAGNAVKHVSFYGLGNGTARGGVP 240
Query: 241 SARIAVYKIC--WADGCYDADILAAFDDAIADGVDIISLSVGGSAPKPYFNDSIAIGAFH 300
+ARIAVYK+C DGC ILAAFDDAIAD VDII++S+GG P+ D IAIGAFH
Sbjct: 241 AARIAVYKVCDPGVDGCTTDGILAAFDDAIADKVDIITISIGGDNSSPFEEDPIAIGAFH 300
Query: 301 AMKHGILTSNSAGNNGPRYFTTSNVSPWSLSVAASSMDRKFVTPVHLDNGNSYQGVSINT 360
AM GIL NSAGN+GP T ++++PW +VAAS+ +R FVT V L NG + G S+N+
Sbjct: 301 AMAKGILIVNSAGNSGPEPSTVASIAPWMFTVAASNTNRAFVTKVVLGNGKTV-GRSVNS 360
Query: 361 FDLHGKQYPLIHARDAPNIAGGFNSSVSRFCVENSVDLNLVRGKIVVCDSLLAPSTVASF 420
FDL+GK+YPL++ + A + G ++ FC +D V+GKIV+CDS P +
Sbjct: 361 FDLNGKKYPLVYGKSASSSCGAASAG---FCSPGCLDSKRVKGKIVLCDSPQNPDEAQAM 420
Query: 421 NGTVGVIMQGTGVKDYASSYPLPASYLQFAASNDI--QLSSATESSATIFKSIAVQDASA 480
++ D AS + P S L N + ++S A + KS + + A
Sbjct: 421 GAIASIVRSHR--TDVASIFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKSETIFNQRA 480
Query: 481 PSVVSFSSRGPNLVTLDILKPDLTAPGVEILAAWPPIAPVSGVVGDSRSVLYNVISGTSM 540
P V S+ SRGPN + DILKPD+TAPG EI+AA+ P AP S + D+R V Y+V +GTSM
Sbjct: 481 PVVASYFSRGPNTIIPDILKPDITAPGSEIVAAYSPDAPPS--ISDTRRVKYSVDTGTSM 540
Query: 541 SCPHVTAIAVYVKTFHPTWSPAAIKSALMTTAFTMNANLHP---QAEFSYGAGHVNPLKA 600
SCPHV +A Y+K+FHP WSP+ I+SA+MTTA+ MNA+ P AEF+YGAGHV+P+ A
Sbjct: 541 SCPHVAGVAAYLKSFHPRWSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDPITA 600
Query: 601 INPGLVYNASESDYIKFLCGEGYTTEMVRLITGDNSACIGGNIGRV-WDLNYPSFALSTT 660
I+PGLVY A++SD+I FLCG YT + +RLI+GD+S+C + +LNYPS +
Sbjct: 601 IHPGLVYEANKSDHIAFLCGLNYTAKNLRLISGDSSSCTKEQTKSLPRNLNYPSMTAQVS 660
Query: 661 PSQSINQFFTRTLTNVESGASIYSATVSAPPSLRIFADPPVLSFNGIGDMKSFRLVVQGT 720
++ F RT+TNV + Y A V L++ P VLS + + KSF + G
Sbjct: 661 AAKPFKVIFRRTVTNVGRPNATYKAKV-VGSKLKVKVVPAVLSLKSLYEKKSFTVTASGA 720
Query: 721 VSQA--VVSASLVWSDGVHQVRSPITVYVVN 734
+A +VSA L+WSDGVH VRSPI VY N
Sbjct: 721 GPKAENLVSAQLIWSDGVHFVRSPIVVYATN 738
BLAST of Lag0040827 vs. TAIR 10
Match:
AT5G03620.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 611.3 bits (1575), Expect = 1.0e-174
Identity = 353/752 (46.94%), Postives = 481/752 (63.96%), Query Frame = 0
Query: 11 FNLFFSLLVSSSHSDNDARKTYIVYMGSKPEDA--SSAHLHHRTMLEQVVGSTFAPEHLL 70
F L + +++++ +N RK YIVYMG E++ +A HH ++ + + A E +
Sbjct: 14 FCLVNNAVIAATEDENVERKPYIVYMGEATENSLVEAAENHHNLLMTVIGDESKARELKI 73
Query: 71 YSYKRSFNGFAVRLTEEEAQKIALKEGVVSVFPNGKKHLHTTRSWDFMGFTQS-VARVKQ 130
YSY ++ NGF RL EA+K++ +EGVVSVF N ++ LHTTRSWDF+G +S R
Sbjct: 74 YSYGKNINGFVARLFPHEAEKLSREEGVVSVFKNTQRQLHTTRSWDFLGLVESKYKRSVG 133
Query: 131 VESNIVVGVLDTGIWPESPSFDDAGLDPPPTKWKGTCQSSTNF-QCNRKIIGARIY--RS 190
+ESNI+VGVLDTGI ESPSF+D G+ PPP KWKG C + NF +CN K+IGA+ + +S
Sbjct: 134 IESNIIVGVLDTGIDVESPSFNDKGVGPPPAKWKGKCVTGNNFTRCNNKVIGAKYFHIQS 193
Query: 191 EEFPPGDIRSPRDSSGHGTHTASTVAGGLVSEASLYGLGSGTARGGVPSARIAVYKICWA 250
E P G+ + D GHGTHT+ST+AG VS ASL+G+ +GTARGGVPSARIA YK+CW
Sbjct: 194 EGLPDGEGDTAADHDGHGTHTSSTIAGVSVSSASLFGIANGTARGGVPSARIAAYKVCWD 253
Query: 251 DGCYDADILAAFDDAIADGVDIISLSVGGSAPKPYFNDSIAIGAFHAMKHGILTSNSAGN 310
GC D D+LAAFD+AI+DGVDIIS+S+GG A P+F D IAIGAFHAMK GILT+ SAGN
Sbjct: 254 SGCTDMDMLAAFDEAISDGVDIISISIGG-ASLPFFEDPIAIGAFHAMKRGILTTCSAGN 313
Query: 311 NGPRYFTTSNVSPWSLSVAASSMDRKFVTPVHLDNGNSYQGVSINTFDLHGKQYPLIHAR 370
NGP FT SN++PW ++VAA+S+DRKF T V L NG + G+S+N F+ K YPL
Sbjct: 314 NGPGLFTVSNLAPWVMTVAANSLDRKFETVVKLGNGLTASGISLNGFNPRKKMYPLTSGS 373
Query: 371 DAPNI-AGGFNSSVSRFCVENSVDLNLVRGKIVVCDSLLAPS---------TVASFNGTV 430
A N+ AGG+ + C ++ + V GK+V C++ V S G
Sbjct: 374 LASNLSAGGYGEPST--CEPGTLGEDKVMGKVVYCEAGREEGGNGGQGQDHVVRSLKG-A 433
Query: 431 GVIMQGTGVKDYASSYPLPASYLQFAASNDI--QLSSATESSATIFKSIAVQDASAPSVV 490
GVI+Q D A+S + SY+ F I ++S A IFK+ + APS+
Sbjct: 434 GVIVQLLEPTDMATSTLIAGSYVFFEDGTKITEYINSTKNPQAVIFKTKTTK-MLAPSIS 493
Query: 491 SFSSRGPNLVTLDILKPDLTAPGVEILAAWPPIAPVSGVVGDSRSVLYNVISGTSMSCPH 550
SFS+RGP ++ +ILKPD++APG+ ILAA+ +A V+G D+R L++++SGTSM+CPH
Sbjct: 494 SFSARGPQRISPNILKPDISAPGLNILAAYSKLASVTGYPDDNRRTLFSIMSGTSMACPH 553
Query: 551 VTAIAVYVKTFHPTWSPAAIKSALMTTAFTMNANLHPQAEFSYGAGHVNPLKAINPGLVY 610
A A YVK+FHP WSPAAIKSALMTTA M + +AE SYG+G +NP +AI+PGLVY
Sbjct: 554 AAAAAAYVKSFHPDWSPAAIKSALMTTATPMRIKGN-EAELSYGSGQINPRRAIHPGLVY 613
Query: 611 NASESDYIKFLCGEGYTTEMVRLITGDNS--------ACIGGNIGRVWD-LNYPSFALST 670
+ +E Y++FLC EGY + + L+TGDNS C G D LNYPS
Sbjct: 614 DITEDAYLRFLCKEGYNSTSIGLLTGDNSNNTTKKEYNCENIKRGLGSDGLNYPSLHKQV 673
Query: 671 TPSQS-INQFFTRTLTNVESGASIYSATVSAPPSLRIFADPPVLSFNGIGDMKSFRLVVQ 730
+++ +++ F RT+TNV G S Y A V AP LR+ P V+SF + ++F++V+
Sbjct: 674 NSTEAKVSEVFYRTVTNVGYGPSTYVARVWAPKGLRVEVVPKVMSFERPKEKRNFKVVID 733
BLAST of Lag0040827 vs. TAIR 10
Match:
AT5G59100.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 602.1 bits (1551), Expect = 6.2e-172
Identity = 344/748 (45.99%), Postives = 472/748 (63.10%), Query Frame = 0
Query: 6 FKFIFFNLFFSLL--VSSSHSDNDARKTYIVYMGSKP-EDASSAHLHHRTMLEQVVGSTF 65
F F+F +L L VS+ D+ ++ YIVY+GS P + + H ++L+++ G +
Sbjct: 7 FIFLFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITGESL 66
Query: 66 APEHLLYSYKRSFNGFAVRLTEEEAQKIALKEGVVSVFPNGKKHLHTTRSWDFMGFTQSV 125
L+ SYK+SFNGFA RLTE E +++A E VVSVFP+ K L TT SW+FMG + +
Sbjct: 67 IENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGI 126
Query: 126 --ARVKQVESNIVVGVLDTGIWPESPSFDDAGLDPPPTKWKGTCQSSTNFQCNRKIIGAR 185
R + +ES+ ++GV+D+GI+PES SF D G PPP KWKGTC NF CN K+IGAR
Sbjct: 127 KTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGAR 186
Query: 186 IYRSEEFPPGDIRSPRDSSGHGTHTASTVAGGLVSEASLYGLGSGTARGGVPSARIAVYK 245
Y ++ ++ RD SGHGTHTAS AG V+ ++ YGLG+GTARGGVP+ARIAVYK
Sbjct: 187 DYTAKSKAN---QTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYK 246
Query: 246 ICWADGCYDADILAAFDDAIADGVDIISLSVGGSAPKPYFNDSIAIGAFHAMKHGILTSN 305
+C +GC +++AFDDAIADGVD+IS+S+ P+ D IAIGAFHAM G+LT N
Sbjct: 247 VCDNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTVN 306
Query: 306 SAGNNGPRYFTTSNVSPWSLSVAASSMDRKFVTPVHLDNGNSYQGVSINTFDLHGKQYPL 365
+AGNNGP+ T ++ +PW SVAAS +R F+ V L +G G S+NT+D++G YPL
Sbjct: 307 AAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPL 366
Query: 366 IHARDAPNIAGGFNSSVSRFCVENSVDLNLVRGKIVVCDSLLAPSTVASFNGTVGVIMQG 425
++ + A + +R C +D LV+GKIV+CDS A G VG I++
Sbjct: 367 VYGKSA--ALSTCSVDKARLCEPKCLDGKLVKGKIVLCDSTKG-LIEAQKLGAVGSIVKN 426
Query: 426 TGVKDYASSYPLPASYLQFAASND------IQLSSATESSATIFKSIAVQDASAPSVVSF 485
D A P S+L SND ++S AT+ KS + + AP V SF
Sbjct: 427 P-EPDRAFIRSFPVSFL----SNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASF 486
Query: 486 SSRGPNLVTLDILKPDLTAPGVEILAAWPPIAPVSGVVGDSRSVLYNVISGTSMSCPHVT 545
SSRGP+ + DILKPD+TAPGVEILAA+ P + + D+R V Y+V+SGTSM+CPHV
Sbjct: 487 SSRGPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVA 546
Query: 546 AIAVYVKTFHPTWSPAAIKSALMTTAFTMNA--NLHPQAEFSYGAGHVNPLKAINPGLVY 605
+A YVKTFHP WSP+ I+SA+MTTA+ MNA + EF+YG+GHV+P+ AINPGLVY
Sbjct: 547 GVAAYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVY 606
Query: 606 NASESDYIKFLCGEGYTTEMVRLITGDNSAC---IGGNIGRVWDLNYPSFALSTTPSQSI 665
+++D+I FLCG YT++ +R+I+GDNS C I + R +LNYP+ + + ++
Sbjct: 607 ELTKADHINFLCGLNYTSDHLRIISGDNSTCTKEISKTLPR--NLNYPTMSAKVSGTKPF 666
Query: 666 NQFFTRTLTNVESGASIYSATVSAPP--SLRIFADPPVLSFNGIGDMKSFRLVVQGTV-- 725
N F RT+TNV S Y+A V P L I P VLS + + +SF + V
Sbjct: 667 NITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIG 726
Query: 726 SQAVVSASLVWSDGVHQVRSPITVYVVN 734
++ VSA+L+WSDG H VRSPI VY ++
Sbjct: 727 TKQPVSANLIWSDGTHNVRSPIIVYAMS 741
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| Q39547 | 2.8e-278 | 66.58 | Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1 | [more] |
| Q9FIF8 | 3.8e-182 | 47.98 | Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S... | [more] |
| Q9STF7 | 4.9e-174 | 46.83 | Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 S... | [more] |
| F4JA91 | 6.4e-174 | 46.60 | Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana OX=3702 GN=SBT4.5 PE=3 S... | [more] |
| Q9LZS6 | 1.4e-173 | 46.94 | Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana OX=3702 GN=SBT4.15 PE=3... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1CBL9 | 0.0e+00 | 79.21 | LOW QUALITY PROTEIN: cucumisin-like OS=Momordica charantia OX=3673 GN=LOC1110101... | [more] |
| A0A1S3CF95 | 0.0e+00 | 79.76 | cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500249 PE=3 SV=1 | [more] |
| A0A6J1CEK6 | 0.0e+00 | 75.88 | cucumisin-like OS=Momordica charantia OX=3673 GN=LOC111010021 PE=3 SV=1 | [more] |
| A0A6J1K719 | 0.0e+00 | 72.90 | cucumisin-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490766 PE=3 SV=1 | [more] |
| A0A6J1IQ27 | 2.7e-310 | 73.38 | cucumisin-like OS=Cucurbita maxima OX=3661 GN=LOC111478984 PE=3 SV=1 | [more] |
