Lag0040812 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0040812 vs. NCBI nr
Match: XP_024041095.1 (uncharacterized protein LOC112098853 [Citrus clementina])

HSP 1 Score: 309.7 bits (792), Expect = 8.7e-80
Identity = 204/578 (35.29%), Postives = 288/578 (49.83%), Query Frame = 0

Query: 456 LEELADQVDPPFTEEVMKAEVPQKFKVPTFKQYDGKKDPVQHLNAYRSWMDFHGVSDAIR 515
           L+++  + +PPFT ++M A+ P +F +P  + YDG++DP +HL  YR+ M+  G S AI 
Sbjct: 166 LDDVTAEHEPPFTSDIMVAKYPPRFHLPQMEPYDGQRDPTEHLELYRTLMEVQGASQAIL 225

Query: 516 CRAFFFTLTGSARHWFERLKRRSISCFKELAQAFLAQFMGAREQRKPHINLLTVKQQPGE 575
           CRAF  TL G+AR WF RL+  SIS F +L++ F + F  AR++ KP   LLTVKQQ GE
Sbjct: 226 CRAFPLTLVGAARRWFRRLQPHSISSFGDLSREFTSHFRSARQRGKPVTYLLTVKQQEGE 285

Query: 576 SLRDYITRFNDEALQVEGYSEGAALVAITAGLEDERLLNSIGKSQPRTYAEFVSRAQKYM 635
           +LRDYI R+N+E  QV+GY +G AL  I  GL   +L  S+ K  P +Y+E ++RA+KY 
Sbjct: 286 TLRDYIRRYNNEITQVDGYDDGIALSGIMEGLRMGKLWWSVSKRPPTSYSEMLARAEKYA 345

Query: 636 SAEELLKSKRSER-EYKRFSSSSYDIKRTKGSGPTKEAGAEQ--------------TMAE 695
           +AEE  K++  E+ E  +      D +  +   P +     Q               +A 
Sbjct: 346 NAEERFKNRNQEKGESTKGKKKKDDQRERRVVRPDQSLRRNQFRPGERSEVRDTRPRLAS 405

Query: 696 ADQTMAEADQTKEHLL----SVSLRNTPQLL--------------FRK-----SKNCIQL 755
                 E +  +E +L    + +L  TP  +              F K     +  C +L
Sbjct: 406 QFTNFTELNTPREQILMQVRNPTLFRTPPPMKTNPSRRNPNKYCHFHKDHGHDTSECFEL 465

Query: 756 KDEIEALIQNGYLKEFVGEPKAEADHGWPRPSLTKDGRDKE---EPLREIRTIFGGPAGG 815
           K++IE+L++ G L+E+V  P+       P  S  + G+ KE   E + ++  I+GGPA G
Sbjct: 466 KEQIESLVRQGQLQEYVRNPEDRVKSEQPESSKKRKGKKKEDSDESMSDVNHIWGGPAAG 525

Query: 816 GSSRKRKAMAREARSEPE------------------------------------------ 875
            S + RK +AR+AR EP                                           
Sbjct: 526 DSRKSRKNLARQARHEPNGPEVNLTNQSQGSMVRGIPIIFSEDDARGVHHPHCDALVVTL 585

Query: 876 ----------------------YRAFKAMRLGMELLKPSLTPLVGFGRERVTPEGSIELP 920
                                    F  M L    LKP  TPL GF    V PEG IEL 
Sbjct: 586 PVANKRLHRILIDNGSSADILFLSTFARMGLERSQLKPYTTPLHGFAGGSVVPEGVIELI 645

BLAST of Lag0040812 vs. NCBI nr
Match: XP_022158830.1 (uncharacterized protein LOC111025293 [Momordica charantia])

HSP 1 Score: 295.0 bits (754), Expect = 2.2e-75
Identity = 244/686 (35.57%), Postives = 323/686 (47.08%), Query Frame = 0

Query: 246 DRHQRRSRDDDSIRGSPRQAGRAEDADIKIAALEDEVKGMNRSLSRIL---QILDKPDPS 305
           +R  RRS DD S +       R    D ++   ED     N+     L     L  P+P 
Sbjct: 4   ERSPRRSDDDCSAK------RRLNLGDPQVGGPEDGTSQPNQERQEGLXEXXALTTPEPF 63

Query: 306 TK---------VHERSLIRDPRKGKEPMEHTAESGTRSRGKKTDSMTSKVRGLKPTDRTI 365
            K           E  L+RDP+KGK P E   E  T S G K      ++ G       I
Sbjct: 64  QKQFAVLEDKESDEVPLVRDPKKGKGPTESDTEESTNSVGSKL-----RIGGNTRQRTRI 123

Query: 366 LRSRESSTLKGRHHTVSTPSYGHTKTDLRNLIVEKRRSAKTAESEAKAVEAEARAAEAEA 425
              R++     + H    P+ G +  D RN             SE  +++          
Sbjct: 124 FDPRKTK----KQHKSPAPNGGKSDHDDRN-------------SEPISLD---------- 183

Query: 426 RAAEAEAKKDDLPWKTELLNALKELGNPQGDQQRSKNFGDQNLEELADQVDPPFTEEVMK 485
                + K  D P  +E  ++ KE G               +LEEL DQ D PFTEE+M+
Sbjct: 184 -----KGKPADRPESSEKRHSHKEKG--------------FDLEELLDQADSPFTEEIMR 243

Query: 486 AEVPQKFKVPTFKQYDGKKDPVQHLNAYRSWMDFHGVSDAIRCRAFFFTLTGSARHWFER 545
            +VP KFK+PT KQ+D   DPV HL+AYR WMD +GVS+A+RCR F  TL GSAR WF +
Sbjct: 244 EKVPPKFKLPTVKQFDXTTDPVDHLDAYREWMDIYGVSEAVRCRVFSTTLNGSARIWFRQ 303

Query: 546 LKRRSISCFKELAQAFLAQFMGAREQRKPHINLLTVKQQPGESLRDYITRFNDEALQVEG 605
           LKR SIS FK LA+AF+ QF+G R + +P   LLT+KQ+  ESLRDY+ RFN+E LQVEG
Sbjct: 304 LKRGSISSFKSLARAFVTQFVGGRCRSRPVAYLLTIKQRTTESLRDYVARFNEEKLQVEG 363

Query: 606 YSEGAALVAITAGLEDERLLNSIGKSQPRTYAEFVSRAQKYMSAEELLKSKRS------- 665
            ++  +L+A  +G+ DE L  S GK  P T++E +SRAQ+YMSA E   SKR        
Sbjct: 364 LTDAVSLLAFMSGVRDEHLSFSFGKRTPNTFSEALSRAQRYMSAGEFFYSKREPDGKRTD 423

Query: 666 ---EREYKRFSSSSYDIKRTKGS---GPTKEAGAEQTMAEADQTMAEADQTK-----EHL 725
              ER   +   S ++ KR + S    P K      T    +Q + E    +     E +
Sbjct: 424 PKRERSGDKPQGSRWE-KRDRSSQKDPPRKFEKYTPTTVPIEQVLMEIKDQRLLKWPERM 483

Query: 726 LSVSLRNTP--QLLFRK-----SKNCIQLKDEIEALIQNGYLKEFVGEPKAEADHGWPRP 785
            + S + +     LF +     +++C  LK+E+E LI+ GYLKE+V EPKA         
Sbjct: 484 KASSAKRSKGRYCLFHRDHGHATQDCFDLKEEVEGLIRRGYLKEYVEEPKA--------- 543

Query: 786 SLTKDGRDKEEPLREIRTIFGGPAGGGSSRKRKAMAREARSEPE---------------- 835
             T++G   + P REIRTI GGP    S RKRKA  REAR+  E                
Sbjct: 544 --TQNGESDKSPAREIRTIMGGPIERESGRKRKADVREARTSREQNEVYHAYTTNRPVTI 603

BLAST of Lag0040812 vs. NCBI nr
Match: XP_024047974.1 (uncharacterized protein LOC112101548 [Citrus clementina] >XP_024953918.1 uncharacterized protein LOC112498174 [Citrus sinensis])

HSP 1 Score: 287.0 bits (733), Expect = 6.0e-73
Identity = 196/577 (33.97%), Postives = 276/577 (47.83%), Query Frame = 0

Query: 457 EELADQVDPPFTEEVMKAEVPQKFKVPTFKQYDGKKDPVQHLNAYRSWMDFHGVSDAIRC 516
           +++  + +PPFT+E+M+A  P  F++P+ + YDG+K P++H+  YRS M+  GVS AI C
Sbjct: 9   DDMMAESEPPFTQEIMRARYPSGFRLPSIEPYDGQKYPMEHVELYRSLMEVQGVSSAILC 68

Query: 517 RAFFFTLTGSARHWFERLKRRSISCFKELAQAFLAQFMGAREQRKPHINLLTVKQQPGES 576
           RAF  TL+ +AR WF  L+  SIS F EL + F   F  AR++ KP   LLTVKQ  GES
Sbjct: 69  RAFPLTLSRAARRWFRMLRPNSISSFNELIREFTMHFHSARQRGKPVTYLLTVKQHEGES 128

Query: 577 LRDYITRFNDEALQVEGYSEGAALVAITAGLEDERLLNSIGKSQPRTYAEFVSRAQKYMS 636
           LR+YI R+N E  QV+GY +G AL  +  GL+  RL  S+ K+ P TY+E +SRA+KY +
Sbjct: 129 LREYIQRYNAETTQVDGYDDGVALSGLMEGLQMGRLWWSVAKNPPSTYSEMLSRAEKYAN 188

Query: 637 AEELLKSKRS-EREYKRFSSSSYDIKRTKGSGPTKEAGAEQTMAEADQ------------ 696
           AEE  +SK+  E+     +  + + +R    G   +A  +      DQ            
Sbjct: 189 AEERFQSKKGREKGESSKNKKTKEDRRDIRPGRPDQASRKDQFRPWDQLSSQEPRSRPPV 248

Query: 697 ---TMAEADQTKEHLL-----SVSLRNTPQLLFRKSK------------------NCIQL 756
                 E +  +EH+L     S   +  P L   +S+                   C  L
Sbjct: 249 QFTNYTELNAPREHILMQMKNSTLFKAPPPLKSDRSRRNQKKYCHFYKDVGHDTSECFDL 308

Query: 757 KDEIEALIQNGYLKEFVGEPKAEADHGWPRPSLTKDGRDKE----EPLREIRTIFGGPAG 816
           K++IE+L++   L+ +V    +  +   P  S    G+ KE    E +  +  I+GGP  
Sbjct: 309 KEQIESLVRQSQLQGYVRGQGSRENRDLPESSRKDKGKKKENYSDENMSVVNHIWGGPFA 368

Query: 817 GGSSRKRKAMAREARSEPE----------------------------------------- 876
           G S + RK +AR+AR EP                                          
Sbjct: 369 GNSGKARKNLARQARHEPNGLDINLTDRLQNSRFRDTPIIFSEEDTRGIHHPHCDAIVVT 428

Query: 877 -----------------------YRAFKAMRLGMELLKPSLTPLVGFGRERVTPEGSIEL 918
                                    AF  M L    L P  T L GF    V PEG IEL
Sbjct: 429 LPVANKRLHRILIDNGSSADILFLSAFTKMGLERSQLMPYTTQLHGFAGGSVIPEGMIEL 488

BLAST of Lag0040812 vs. NCBI nr
Match: GFS31826.1 (hypothetical protein Acr_00g0019390 [Actinidia rufa])

HSP 1 Score: 276.9 bits (707), Expect = 6.2e-70
Identity = 205/553 (37.07%), Postives = 288/553 (52.08%), Query Frame = 0

Query: 386 VEKRRSAKTAESEAKAVEAEARAAEAEARAAEA-EAKKDDLP-WKTELLNALKELGNPQG 445
           V  RR+         A EA++ A +++A+   A E   +  P W  ++     +L    G
Sbjct: 140 VPLRRNLHQTGVGPNADEAQSHAVQSKAKTVVALEHTGETRPIWDEQMRQMQAQLA---G 199

Query: 446 DQQRSKNFGDQNLEELADQVDPPFTEEVMKAEVPQKFKVPTFKQYDGKKDPVQHLNAYRS 505
                K  G  ++EEL ++ D PFT  VM+  +P KFK+PT + +DG KDP+ HL  Y++
Sbjct: 200 VIAAVKEKGTNSVEELINRTDTPFTPAVMRVSLPLKFKMPTLEPFDGTKDPLDHLETYKA 259

Query: 506 WMDFHGVSDAIRCRAFFFTLTGSARHWFERLKRRSISCFKELAQAFLAQFMGAREQRKPH 565
            MD   V D I CRAF  TL GS R WF RL   +IS F +L++ F+  ++G + Q+KP 
Sbjct: 260 LMDLQAVPDEIMCRAFPTTLKGSTRIWFHRLPPGTISKFVDLSRLFVGHYIGGQRQKKPS 319

Query: 566 INLLTVKQQPGESLRDYITRFNDEALQVEGYSEGAALVAITAGLEDERLLNSIGKSQPRT 625
            +L  VKQ+  E+LR+++T FN E L V+   +  A+ A  AG+   + L S+ K  P+T
Sbjct: 320 TSLFNVKQREKETLREFVTLFNQETLYVDDLDQKVAVAAFIAGVGTPKFLFSLAKEAPKT 379

Query: 626 YAEFVSRAQKYMSAEELLKSKR------SEREYKRFSSSSYDIKRTK-----------GS 685
            A  + RAQKYM+AE+ LK++       S  + KR      D K TK             
Sbjct: 380 MAALMLRAQKYMNAEDTLKAREDLEGTISIPDRKRSIVERKDDKVTKVPKLTEQKLTIEG 439

Query: 686 GPTKEAGAEQTM----AEADQTMAEADQTKEHLLSVSLRNTPQLLFR------------K 745
             TK A   Q         DQ + E  +         +R+ P+   +            +
Sbjct: 440 NRTKPARYNQYFTPLNTSLDQVLYEIKEDPALKWPGRMRSPPEKRPKNKWCRFHQDHGHE 499

Query: 746 SKNCIQLKDEIEALIQNGYLKEFV--GEPKAEADHG---WPRPSLTKDGRDKEEPLREIR 805
           +++C  LK ++E LI+ G L  F+  G P  + DH      R SL K+ +  ++P+ EI+
Sbjct: 500 TEDCYDLKQQVENLIKQGRLGRFIAWGRPDKKRDHSPQRVERRSLEKE-KGNQQPIGEIK 559

Query: 806 TIFGGPAGGG-SSRKRKAMAREARSEPEYRAFKAMRLGMELLKPS--LTPLVGFGRERVT 865
            I GG AGGG SS  RK  ARE R++ E      M +G    +P    + LVGF  +RVT
Sbjct: 560 VISGGFAGGGESSCSRKKHAREIRNQKE-----VMSIGQSAKRPRPVHSLLVGFTGDRVT 619

Query: 866 PEGSIELPVTFGDGQNAVTKMINFLVVDCMSAYNAILGRPTLHEMKAIASTYHQLLKFPT 896
           P GSI LPVT GD     TKM+ FLVVDC SAYN I+GR  L+E KAI STYH  +KFPT
Sbjct: 620 PLGSIALPVTLGDAPRQTTKMVTFLVVDCPSAYNIIVGRTALNEFKAIPSTYHLKVKFPT 679

BLAST of Lag0040812 vs. NCBI nr
Match: XP_030963320.1 (uncharacterized protein LOC115984434 [Quercus lobata])

HSP 1 Score: 268.1 bits (684), Expect = 2.9e-67
Identity = 163/482 (33.82%), Postives = 254/482 (52.70%), Query Frame = 0

Query: 456 LEELADQVDPPFTEEVMKAEVPQKFKVPTFKQYDGKKDPVQHLNAYRSWMDFHGVSDAIR 515
           +E+L  + D PF   +    +P KFK+P+   YDG +DP  H+  +++ M   GV D I 
Sbjct: 119 VEDLVHRTDSPFVPSINAHPLPPKFKMPSLDSYDGTRDPFDHIATFKTTMHLQGVPDEIM 178

Query: 516 CRAFFFTLTGSARHWFERLKRRSISCFKELAQAFLAQFMGAREQRKPHINLLTVKQQPGE 575
           CRAF  TL G AR WF ++   +++ F+EL++ F+  F+G +  ++   +LLT++Q+  E
Sbjct: 179 CRAFPITLKGPARVWFSKIPPSTVTSFEELSKLFVNNFIGGQRHKRSSSSLLTIEQEENE 238

Query: 576 SLRDYITRFNDEALQVEGYSEGAALVAITAGLEDERLLNSIGKSQPRTYAEFVSRAQKYM 635
           SLR +ITRFN EAL V+   +   L A   G+  +  ++ + + +P+T AE V  AQ +M
Sbjct: 239 SLRSFITRFNREALAVDEMDDKLLLAAFHNGVHSDLFIHKLYEQEPQTMAELVHSAQNFM 298

Query: 636 SAEELLKSKRSEREYKRFSSSSYDIKRTKGSGPTKEAGAEQTMAEADQTMAEADQTKEHL 695
           +AE+ + +K+     KR      +  R    GP  + G  +   + D+    + +++++ 
Sbjct: 299 NAEDAIIAKKR----KRIEKMDANPTRHSEQGPRLKKGRTEDKKDRDRKAGPSARSQQYT 358

Query: 696 --------LSVSLRNTPQLLF------------------------RKSKNCIQLKDEIEA 755
                   + + +++ P L +                          +  C  LK +IE 
Sbjct: 359 PLNMPFDQVLMQIKDDPSLKWLEKMKGDPNKCNRNKYCRFHRDHGHDTDECFDLKQQIEN 418

Query: 756 LIQNGYLKEFVG-EPKAEADHGWPRPSLTKDGRDKEEPLREIRTIFGGPAGGGSSRKRKA 815
           LI+ G L+ F+G + K E   G       K       PL EIR I GG +   SSR+   
Sbjct: 419 LIRQGKLRSFLGRDHKDEKLKG-------KAEESSRPPLGEIRVIVGGSSTVQSSRRVLV 478

Query: 816 MAREARSEPEYRAFKAMRLGMELLKPSLTPLVGFGRERVTPEGSIELPVTFGDGQNAVTK 875
               +     + AF+ M+LG + L+P  +PLVGFG  +V P G++ LPV  G     VTK
Sbjct: 479 DNGSSADILYFPAFQQMKLGRDRLRPVNSPLVGFGGMKVQPVGTVTLPVVVGAYPQQVTK 538

Query: 876 MINFLVVDCMSAYNAILGRPTLHEMKAIASTYHQLLKFPTPNGVGVVKGEQKESRECYWT 905
            ++FLVVDC S+YNAI+GRPTL+  K + STYH  +KFPT  GVG V+G+Q  +RECY  
Sbjct: 539 DVSFLVVDCSSSYNAIIGRPTLNSWKVVTSTYHLSVKFPTEYGVGQVQGDQLAARECYLA 589

BLAST of Lag0040812 vs. ExPASy TrEMBL
Match: A0A6J1DWY0 (uncharacterized protein LOC111025293 OS=Momordica charantia OX=3673 GN=LOC111025293 PE=4 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.1e-75
Identity = 244/686 (35.57%), Postives = 323/686 (47.08%), Query Frame = 0

Query: 246 DRHQRRSRDDDSIRGSPRQAGRAEDADIKIAALEDEVKGMNRSLSRIL---QILDKPDPS 305
           +R  RRS DD S +       R    D ++   ED     N+     L     L  P+P 
Sbjct: 4   ERSPRRSDDDCSAK------RRLNLGDPQVGGPEDGTSQPNQERQEGLXEXXALTTPEPF 63

Query: 306 TK---------VHERSLIRDPRKGKEPMEHTAESGTRSRGKKTDSMTSKVRGLKPTDRTI 365
            K           E  L+RDP+KGK P E   E  T S G K      ++ G       I
Sbjct: 64  QKQFAVLEDKESDEVPLVRDPKKGKGPTESDTEESTNSVGSKL-----RIGGNTRQRTRI 123

Query: 366 LRSRESSTLKGRHHTVSTPSYGHTKTDLRNLIVEKRRSAKTAESEAKAVEAEARAAEAEA 425
              R++     + H    P+ G +  D RN             SE  +++          
Sbjct: 124 FDPRKTK----KQHKSPAPNGGKSDHDDRN-------------SEPISLD---------- 183

Query: 426 RAAEAEAKKDDLPWKTELLNALKELGNPQGDQQRSKNFGDQNLEELADQVDPPFTEEVMK 485
                + K  D P  +E  ++ KE G               +LEEL DQ D PFTEE+M+
Sbjct: 184 -----KGKPADRPESSEKRHSHKEKG--------------FDLEELLDQADSPFTEEIMR 243

Query: 486 AEVPQKFKVPTFKQYDGKKDPVQHLNAYRSWMDFHGVSDAIRCRAFFFTLTGSARHWFER 545
            +VP KFK+PT KQ+D   DPV HL+AYR WMD +GVS+A+RCR F  TL GSAR WF +
Sbjct: 244 EKVPPKFKLPTVKQFDXTTDPVDHLDAYREWMDIYGVSEAVRCRVFSTTLNGSARIWFRQ 303

Query: 546 LKRRSISCFKELAQAFLAQFMGAREQRKPHINLLTVKQQPGESLRDYITRFNDEALQVEG 605
           LKR SIS FK LA+AF+ QF+G R + +P   LLT+KQ+  ESLRDY+ RFN+E LQVEG
Sbjct: 304 LKRGSISSFKSLARAFVTQFVGGRCRSRPVAYLLTIKQRTTESLRDYVARFNEEKLQVEG 363

Query: 606 YSEGAALVAITAGLEDERLLNSIGKSQPRTYAEFVSRAQKYMSAEELLKSKRS------- 665
            ++  +L+A  +G+ DE L  S GK  P T++E +SRAQ+YMSA E   SKR        
Sbjct: 364 LTDAVSLLAFMSGVRDEHLSFSFGKRTPNTFSEALSRAQRYMSAGEFFYSKREPDGKRTD 423

Query: 666 ---EREYKRFSSSSYDIKRTKGS---GPTKEAGAEQTMAEADQTMAEADQTK-----EHL 725
              ER   +   S ++ KR + S    P K      T    +Q + E    +     E +
Sbjct: 424 PKRERSGDKPQGSRWE-KRDRSSQKDPPRKFEKYTPTTVPIEQVLMEIKDQRLLKWPERM 483

Query: 726 LSVSLRNTP--QLLFRK-----SKNCIQLKDEIEALIQNGYLKEFVGEPKAEADHGWPRP 785
            + S + +     LF +     +++C  LK+E+E LI+ GYLKE+V EPKA         
Sbjct: 484 KASSAKRSKGRYCLFHRDHGHATQDCFDLKEEVEGLIRRGYLKEYVEEPKA--------- 543

Query: 786 SLTKDGRDKEEPLREIRTIFGGPAGGGSSRKRKAMAREARSEPE---------------- 835
             T++G   + P REIRTI GGP    S RKRKA  REAR+  E                
Sbjct: 544 --TQNGESDKSPAREIRTIMGGPIERESGRKRKADVREARTSREQNEVYHAYTTNRPVTI 603

BLAST of Lag0040812 vs. ExPASy TrEMBL
Match: A0A2N9J5E0 (Reverse transcriptase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS59595 PE=4 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 6.1e-71
Identity = 204/590 (34.58%), Postives = 297/590 (50.34%), Query Frame = 0

Query: 411 AEARAAEAEAKKDDLPWKTELLNALKELGNPQGDQQRSKNFGDQNLEELADQVDPPFTEE 470
           A+ARAA+ E          E L  ++E       Q ++K    +NL+ L  + + PFT+ 
Sbjct: 80  ADARAAKLE----------EELKEMREQMKEMKSQVKAK--AAKNLDMLVHRSESPFTKR 139

Query: 471 VMKAEVPQKFKVPTFKQYDGKKDPVQHLNAYRSWMDFHGVSDAIRCRAFFFTLTGSARHW 530
           + +  +P KFKVP  + +DG KDP+ +L+++R+ M  HGVSD I CRAF   L GSAR W
Sbjct: 140 IDEYPLPAKFKVPQLETFDGFKDPLDYLDSFRTIMRLHGVSDEIMCRAFPTNLRGSARTW 199

Query: 531 FERLKRRSISCFKELAQAFLAQFMGAREQRKPHINLLTVKQQPGESLRDYITRFNDEALQ 590
           F +L+  SI  F +L++AF+  F+G R   +P   LL V+Q+ GESLR Y+ RFN EA+Q
Sbjct: 200 FNQLETGSIDTFAQLSRAFIDNFIGGRRSARPANYLLNVRQREGESLRSYVQRFNKEAVQ 259

Query: 591 VEGYSEGAALVAITAGLEDERLLNSIGKSQPRTYAEFVSRAQKYMSAE-------ELLKS 650
           ++  +E  AL A  AGL     L  + K  P++ +E +  AQK+++AE       E L  
Sbjct: 260 IDEPNEYVALTAFNAGLLKGDFLFQLCKDPPKSMSELMYEAQKFINAEDAFEARDEFLSR 319

Query: 651 KRSEREYKRF-----SSSSYDIKRTKGSGPTKEAGAEQTMA-------EADQTMAEADQT 710
           KR E E +RF      SS  D  R +    +     E+  +         DQ + +    
Sbjct: 320 KRKEPEDRRFDSSRNKSSKQDYPRAERKNISSSNRREERPSSFTPLNMSIDQVLLQIQDN 379

Query: 711 KEHLLSVSLRNTP-----QLLFR-------KSKNCIQLKDEIEALIQNGYLKEFVGEPKA 770
            +      LR+ P      L  R        +++C  LK +IEALI+ G L +FV + K 
Sbjct: 380 PDIKWPGKLRSDPTKRSKDLYCRFHRDHGHTTEDCFALKQQIEALIRQGKLGKFVRQDKP 439

Query: 771 EADHGWPRPSLTKDGRDKEEP-----LREIRTIFGGPAGGGSSR-KRKAMAREAR----- 830
           E  H  PRP    + +D++E      + EIRTI GG A GG+SR  RKA AR+A      
Sbjct: 440 EVRHE-PRPPRQDENKDRQEDRPRDIIGEIRTIVGGLASGGASRSSRKAYARQAHNILIT 499

Query: 831 ----------------SEPEYR-------------------------------------- 890
                           SE + R                                      
Sbjct: 500 QRSRKSLKMDDQVISFSEDDARNIHHPYDDALVVTLTIAGFLTRRVLIDNGSSADIIYLP 559

Query: 891 AFKAMRLGMELLKPSLTPLVGFGRERVTPEGSIELPVTFGDGQNAVTKMINFLVVDCMSA 905
           A++ M++  E LKP   PLVGF  ++V P G + L +  G     V   + FLVVDC SA
Sbjct: 560 AYQQMKIDKEQLKPIDIPLVGFTGDKVKPLGVVSLIIEAGTYPKQVRTSVEFLVVDCPSA 619

BLAST of Lag0040812 vs. ExPASy TrEMBL
Match: A0A7J0DBQ5 (Retrotrans_gag domain-containing protein OS=Actinidia rufa OX=165716 GN=Acr_00g0019390 PE=4 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 3.0e-70
Identity = 205/553 (37.07%), Postives = 288/553 (52.08%), Query Frame = 0

Query: 386 VEKRRSAKTAESEAKAVEAEARAAEAEARAAEA-EAKKDDLP-WKTELLNALKELGNPQG 445
           V  RR+         A EA++ A +++A+   A E   +  P W  ++     +L    G
Sbjct: 140 VPLRRNLHQTGVGPNADEAQSHAVQSKAKTVVALEHTGETRPIWDEQMRQMQAQLA---G 199

Query: 446 DQQRSKNFGDQNLEELADQVDPPFTEEVMKAEVPQKFKVPTFKQYDGKKDPVQHLNAYRS 505
                K  G  ++EEL ++ D PFT  VM+  +P KFK+PT + +DG KDP+ HL  Y++
Sbjct: 200 VIAAVKEKGTNSVEELINRTDTPFTPAVMRVSLPLKFKMPTLEPFDGTKDPLDHLETYKA 259

Query: 506 WMDFHGVSDAIRCRAFFFTLTGSARHWFERLKRRSISCFKELAQAFLAQFMGAREQRKPH 565
            MD   V D I CRAF  TL GS R WF RL   +IS F +L++ F+  ++G + Q+KP 
Sbjct: 260 LMDLQAVPDEIMCRAFPTTLKGSTRIWFHRLPPGTISKFVDLSRLFVGHYIGGQRQKKPS 319

Query: 566 INLLTVKQQPGESLRDYITRFNDEALQVEGYSEGAALVAITAGLEDERLLNSIGKSQPRT 625
            +L  VKQ+  E+LR+++T FN E L V+   +  A+ A  AG+   + L S+ K  P+T
Sbjct: 320 TSLFNVKQREKETLREFVTLFNQETLYVDDLDQKVAVAAFIAGVGTPKFLFSLAKEAPKT 379

Query: 626 YAEFVSRAQKYMSAEELLKSKR------SEREYKRFSSSSYDIKRTK-----------GS 685
            A  + RAQKYM+AE+ LK++       S  + KR      D K TK             
Sbjct: 380 MAALMLRAQKYMNAEDTLKAREDLEGTISIPDRKRSIVERKDDKVTKVPKLTEQKLTIEG 439

Query: 686 GPTKEAGAEQTM----AEADQTMAEADQTKEHLLSVSLRNTPQLLFR------------K 745
             TK A   Q         DQ + E  +         +R+ P+   +            +
Sbjct: 440 NRTKPARYNQYFTPLNTSLDQVLYEIKEDPALKWPGRMRSPPEKRPKNKWCRFHQDHGHE 499

Query: 746 SKNCIQLKDEIEALIQNGYLKEFV--GEPKAEADHG---WPRPSLTKDGRDKEEPLREIR 805
           +++C  LK ++E LI+ G L  F+  G P  + DH      R SL K+ +  ++P+ EI+
Sbjct: 500 TEDCYDLKQQVENLIKQGRLGRFIAWGRPDKKRDHSPQRVERRSLEKE-KGNQQPIGEIK 559

Query: 806 TIFGGPAGGG-SSRKRKAMAREARSEPEYRAFKAMRLGMELLKPS--LTPLVGFGRERVT 865
            I GG AGGG SS  RK  ARE R++ E      M +G    +P    + LVGF  +RVT
Sbjct: 560 VISGGFAGGGESSCSRKKHAREIRNQKE-----VMSIGQSAKRPRPVHSLLVGFTGDRVT 619

Query: 866 PEGSIELPVTFGDGQNAVTKMINFLVVDCMSAYNAILGRPTLHEMKAIASTYHQLLKFPT 896
           P GSI LPVT GD     TKM+ FLVVDC SAYN I+GR  L+E KAI STYH  +KFPT
Sbjct: 620 PLGSIALPVTLGDAPRQTTKMVTFLVVDCPSAYNIIVGRTALNEFKAIPSTYHLKVKFPT 679

BLAST of Lag0040812 vs. ExPASy TrEMBL
Match: A0A2N9IPA1 (Ribonuclease H OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS55248 PE=4 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 5.1e-70
Identity = 197/590 (33.39%), Postives = 303/590 (51.36%), Query Frame = 0

Query: 365 RHHTVSTPSYGHTKTDLRNLIVEKRRSAKTAESEAKAVEAEARAAEAEARAAEAEAKKDD 424
           R+ T    S  H + D       + R        ++  +    AA+A A   E E K   
Sbjct: 73  RNETGRNQSQNHNQND-EGSSANQNREPHPHPHPSQVADPAKSAADARAAKLEEELK--- 132

Query: 425 LPWKTELLNALKELGNPQGDQQRSKNFGDQNLEELADQVDPPFTEEVMKAEVPQKFKVPT 484
                E+   +KE+      + + K    +NL+ L  + + PFT+ + +  +P KFKVP 
Sbjct: 133 -----EMREQMKEM------KSQVKAKAAKNLDMLVHRSESPFTKRIDEYPLPAKFKVPQ 192

Query: 485 FKQYDGKKDPVQHLNAYRSWMDFHGVSDAIRCRAFFFTLTGSARHWFERLKRRSISCFKE 544
            + +DG KDP+ +L+++R+ M  HGVSD I CR F   L GSAR WF +L+  SI  F +
Sbjct: 193 LETFDGFKDPLDYLDSFRTIMRLHGVSDEIMCRTFPTNLRGSARTWFNQLETGSIDTFAQ 252

Query: 545 LAQAFLAQFMGAREQRKPHINLLTVKQQPGESLRDYITRFNDEALQVEGYSEGAALVAIT 604
           L++AF+  F+G R   +P   LL ++Q+ GESLR Y+ RFN EA+Q++  +E  AL A  
Sbjct: 253 LSRAFIDNFIGGRRSVRPANYLLNIRQREGESLRSYVQRFNKEAVQIDEPNEYVALTAFN 312

Query: 605 AGLEDERLLNSIGKSQPRTYAEFVSRAQKYMSAEELLKSKRSEREYKRFSSSSYDIKRTK 664
           AGL     L  + K  P++ +E +  AQK+++AE+  ++ R E++Y +    +      +
Sbjct: 313 AGLLKGDFLFQLCKDPPKSMSELMYEAQKFINAEDAFQA-RDEQDYPKAERKNISSSNRR 372

Query: 665 GSGPTKEAGAEQTMAEADQTMAEADQTKEHLLSVSLRNTP-----QLLFR-------KSK 724
              P+       ++   DQ + +     +      LR+ P      L  R        ++
Sbjct: 373 EERPSSFTPLNMSI---DQVLLQIQDNPDIKWPGKLRSDPTKRSKDLYCRFHRDHGHTTE 432

Query: 725 NCIQLKDEIEALIQNGYLKEFVGEPKAEADHGWPRPSLTKDGRDKEEP-----LREIRTI 784
           +C  LK +IEALI+ G L +FV + K E  H   RP    + +D++E      + EIRTI
Sbjct: 433 DCFALKQQIEALIRQGKLGKFVRQDKPEVRHE-SRPPRQDENKDRQEDRPRDIIGEIRTI 492

Query: 785 FGGPAGGGSSR-KRKAMAREA-------RSEPEYR------------------------- 844
            GG A GG+SR  RKA AR+A       RS    +                         
Sbjct: 493 VGGLASGGASRSSRKAYARQAHNILVTQRSRKSLKMDDQDFLTRRVLIDNGSSADIIYLP 552

Query: 845 AFKAMRLGMELLKPSLTPLVGFGRERVTPEGSIELPVTFGDGQNAVTKMINFLVVDCMSA 904
           A++ M++  E LKP   PLVGF  ++V P G + L +  G     V   + FLVVDC SA
Sbjct: 553 AYQQMKIDKEQLKPIDIPLVGFTGDKVKPLGVVSLIIEAGTYPKQVRTSVEFLVVDCPSA 612

BLAST of Lag0040812 vs. ExPASy TrEMBL
Match: A0A2N9IGX8 (Ribonuclease H OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS51171 PE=4 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 2.6e-69
Identity = 209/646 (32.35%), Postives = 318/646 (49.23%), Query Frame = 0

Query: 328 GTRSRGKKTDSMTSKVRGLKPTDRTILRSRESSTLKGRHHTVSTPSYGHTKTDLRNLIVE 387
           G RS  ++ DS     R     +RT  RSR  +   G   + + PS   T    R+    
Sbjct: 65  GNRSNDQEADSHQVTRRRDGEIERTPPRSRHRAESAG---SSAPPSQHKTAKTTRS---- 124

Query: 388 KRRSAKTAESEAKAVEAEARAAEAEARAAEAEAKKDDLPWKT-ELLNALKELGNPQGDQQ 447
             +  +  +  +K +E E R      R    E    DL  KT  L   L+E+    GD +
Sbjct: 125 -SKQPERPDRSSKHLEREDRRGSPSRRPDHPERSSRDLDDKTARLEKELREMRKQMGDMK 184

Query: 448 RS-KNFGDQNLEELADQVDPPFTEEVMKAEVPQKFKVPTFKQYDGKKDPVQHLNAYRSWM 507
            S +    +NL+ L  + D PF   +    +P +FKVP  + +DG KDP  +L A++  M
Sbjct: 185 NSLRAKAARNLDNLVHRADSPFIPSIADFPLPSRFKVPLLENFDGTKDPFDYLEAFKKIM 244

Query: 508 DFHGVSDAIRCRAFFFTLTGSARHWFERLKRRSISCFKELAQAFLAQFMGAREQRKPHIN 567
               VS+ + CRAF   L GSAR WF +L+  SI  F +L++AF+  F+G++ + +P  +
Sbjct: 245 QLQAVSEEVMCRAFPLGLRGSARVWFNKLESESIGSFVQLSRAFIDHFIGSQRRGRPPTH 304

Query: 568 LLTVKQQPGESLRDYITRFNDEALQVEGYSEGAALVAITAGLEDERLLNSIGKSQPRTYA 627
           LL+VKQ  GESLR ++ RFN+EA++++   E   + A  AGL     L  + K  P T +
Sbjct: 305 LLSVKQMEGESLRTFVHRFNEEAMKIDRPKEDVTVTAFMAGLRKGDFLYELCKDPPETMS 364

Query: 628 EFVSRAQKYMSAEELLKS-------KRSEREYKRFSSSSYDI---------KRTKGSGPT 687
           E +  A K+M+AE+ L++       +R E E ++   +   +         KRT  + P 
Sbjct: 365 ELMYEATKHMNAEDALEAMDDPPPKRRKEAEDRKPEPAKQKVPKFTETPERKRTT-APPV 424

Query: 688 KEAGAEQTMAEADQTMAEADQTKEHLLSVSLRNTPQ-------LLFRKS-----KNCIQL 747
           K +         D+ + +            +R+ P          F +      + C+ L
Sbjct: 425 KFSSFTPLNTPIDKLLLQIQDDSSLWWPGKIRSDPNSRPKNLYCRFHRDHGHLIEECVAL 484

Query: 748 KDEIEALIQNGYLKEFVGEPKAEADHGWPRPSLTKDGRDKEE-----PLREIRTIFGGPA 807
           K++IE LI+ G L+++V  P         RP+     R++ E     P+ EIRTI GGPA
Sbjct: 485 KEQIETLIRQGKLQKYVSRPTN------TRPAKPPAQREQAEHNRPGPVGEIRTIVGGPA 544

Query: 808 GGGSSR-KRKAMAR-EARSEPE--------------------------------YRAFKA 867
            GG+SR  RKA AR +AR   +                                  A++ 
Sbjct: 545 SGGTSRASRKAYARQDARGTHQPHDDALVITINIAGFTTRRVMVDNGSSADILYLPAYQQ 604

Query: 868 MRLGMELLKPSLTPLVGFGRERVTPEGSIELPVTFGDGQNAVTKMINFLVVDCMSAYNAI 905
           MRL  + L+P   PLVGF  ++V P G + LP+T G     V+K ++FLVV+C SAYNAI
Sbjct: 605 MRLDKDKLRPMDAPLVGFTGDKVCPVGIVTLPITVGTHPKTVSKTVDFLVVNCPSAYNAI 664