Lag0040755 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0040755
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionprotein ALP1-like
Locationchr13: 7940955 .. 7945695 (+)
RNA-Seq ExpressionLag0040755
SyntenyLag0040755
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGACCCATTAGAGGGTTCAAGAGGAAGAAGAAGACTGAGAAGAAGGTTGACCAAAATGTCTTGGCTGCTTCTTCACTAACGTCTCAGCCCCAGCCCTTGGATTGGTGGGATGAGTTCTCTCAGAGGATTACTGGTAAACTATTTTGCTTCTCTTTGGACTGTGCCTCGTCTTTGTTACTTGTTAATTAATTTTGCTCTCTCTGTTGCTCGTTTATTGAATTTGTGCATTGCCATTGCTCTTTGTTGATTGCTGTTTATTGGGTCTATCGATTGCAATTTTGTGTTTTGCTGAGGTTTTTGGACGGCTGTGGAATGTAGTGTTTAGCAATCATATATCTTCGGTTGTGGGCATAAATGTTGAGTTCAGCAATTATTGAACCGTGATATTGTTCGTCTTAATCTGTAAATTCTCGAACTCTTTCTTTGATTCTTAATATTATCGAGAGGGTCAGAAGGGGCTGGCTGTTGAAGTGATCCTATGAGCATTACTGCTTTTGGAGCTCTGTGGAGATGGATCAAATCTGTTTAGCAGCAGTTATTAACTTAGATATGTTAGTTCACTTGATAATTGAGGTGTACAAGTAGTGGCTACTCATTTAGCTGGTTTATGAGTGTTAACTGTGATGCACTGTGAATGATCTTCGTATTTTTCTCATTGAATTGTTGAAATTTGAAGGTGAAGTTGGTGTGGTAAGTTTTTGCTTCTAAGGAGTTATGTCTTCCAATTGTCGGTTAGGACATTATCCGATTTTGATTGGATTATTATACTATATCTACATTGAGATTAACTTTATATAGAAATAATGACAATGCTGCTTGAAGAATGGGTAATCACTTACAAACTGATTGATTATTGTGACATAATATTAATTTTTTCTTAGCTGCTCCTGCTATTTTAAGCTTTATCACACTTAAGCATGTTTGCTAATTTCATGATTTAGCATTCCCCTTAATTGACTGGCTAGATTATCTAGTTTGGGGCACTAACTTAATCTATGGGTCATATTCAACAAAGTTCTAGAAACGGGTAAAATTGATTTCTTATATTGACTCTCCTCTGATATTCACTTAGTATAGAAATTATCCATTTGGCACCATTCATTTTTACGGATCGAATTCTAAAAGAAAATTTTCATAACTCCTTCTACTTGTCTACTACAATCTCCAAAGGCCTTCCTACTTCTGTGGGGTGAGCATGAGTCGGCCTTCCATCCTGTTGTATGAATTTTACTCCAAATGTCCTCTTGGTTTACAGCAATGATTTTGTTTAATGCAAGTAAATAATAATAATATAACATAAATATGCATTATCATCAACTATTTGATCTTTGCCAACATCAATTGGAAGTTCCCATATAATTTTTTGTTTCAGCATGGCTGGCAATAAATATTTTTAGAGAATCAAGTTCACAATAATCCGCAACTCTCGTTTCCTTCTTTATTGATTCGTAATAAAGATATCACTCTAAACATACTACATTGAAGTTAGAATCTGTCATCTGTTGATATTTGTAAGAAGGCAGTGACTGAAAAATCGAAGGATGACTCTATAAGTAATAATTTATATAATGCAGTGAAGAAGGGCAGGGATGAACAGTCTTCAACTTCAAGTATGTGTTTCTTATGGTCTGCTGTTGTAGATGTATAGAAAACAAGAGAAATAGATTAATATGTTGTTCATGTCAATATAGTATCTTAGAATTTAGAAGTCATGCTAAACAATGATATTCTGAGAGCCAATTTTCTTGGTTAGTATACTTTGTTCTACTGTTAAAATAGTCATGGTTGATGGCAGTCAAATTCAATTCATTGCATGTTGTTTAACATAAAGTAACCTGATTTAGCTGAAATTTTAGATTATTATTCTAGAGATCTTGTTGTGACTTTTGTATCTTAGACAACCTCTTTATAATAATTAATTAATTTAAGTAATGTAGCATCTTTATCCTGTTTCTTTTGAGGCATCTTGGAAATGGGTATGCTTAAGATTCGATTTGATTCATTGTAGTAATGCTGCAAAGCATAAAAGAACATAGGAAACCGTACTGGGTCTCTCCACGGTTTTAAGATTTGTTTCGACATACTGATATGACTAATGCTCAAGATCTGTTTTTGGTATTCAAGAAGTGGATATTTTCTTTACAATCCGAGTTTTTCGCCCAACACTTTTCAGGAGTTTCGAATGACAAGAAATCTGGAATTTTAGGACAGCCCATATTTTTAGTACCTGTACGGCTGTACCTATCCTTCGATTGAATTCACAAATGAGACAGAATTTTAGAGCACGTGTAGAAATTTTAGGTCCCATCATTTGGAAACAAATATATTGGAAAATTCTATCGATTTCTAAATGTCCCTGACAGAAAACATTGTTTCTTGACATGCAAAGGCTTTATTAGGACAGCAGTTGTTGAAGTTTTTTTAGTGAAATAATCAAGAAAGGATGTAAAACCAGATTTCTTTTTTGTTTTTTTCTTGCATGATCCCTTTGGAATTAGAGTTTATAGAAAATTGTTTACTCAATGGATTTTTCTCCATTAACAGTAAGCTCATTCATTTTATCATGATCTATCCTCCATATTTCATGTCTAATGTTCTTCATCCTTTCTTTTTGCTTGCTTGTTCCTCCCTTTTGCCCATTGCATTTTAGTATATTAGCACTCTTTACAAATTGGACATGTACTTCTTCCATGTCACATTTGCTTTCAATTGTTATAGTAACATGTTTCAGGCACCTATTTTAGCAAAGATTCTTATGCCACCTATTCGAACACAATCATTTTCTAGTATGCATTAACTTTTGTACTTTAGCAAAGAGCCTAATGGTACAACTTTAATCACAGTCCCAGAAATACTAAGATGTTGGCAATTGAAACAGCAAGCAAAGTATTTGAAGTTATAAAAGAACCTGATAGCATGATGAAAGTAGGTAGATTTTGTAGATGCCAGTTAGATATCATCCAGCTATATCTGTTTAGTTTTACTAGATACCATCTATTCTATTTTCATATTTCATGGCTCTGAAATGTTGGTTACAGTATAAGATGATTTGGACGTCATAAACTGAATGGCCACTATAAATTTTAGTTCATGTGAAGGCTTTAATTTTTTATTGTCAGGTATTTTTCCGTAGCATCAGTCTATGTAGAAAGATGAAAATTATCATTCATGTTAATGTTGATCAAGTGATCAGTCTGTTGATACCTTGTTCTGGCCATAATTTTGTATGCAATAAGCAAATCTTTGCTTTTAGCTTAAAATCAAAGGATTTGACATAATATGTGGTCAAATGACTCAGTCAGCTTTTATCTTGTTGAATTGAGTATAACTACAGTATTTCTTCTGGCTTTATGCTCACAAAATTTCACTTTATCATTGTTTGGTGATCAATGAATTGGACATCTTGGTCTGATAGTAATTAGCCTCACTAATAATGTCGGTTGTTATATCTTTACTATGAATTATTTCTCCTTAGAAAGTACAATGTCATGCAGTAAATAGTTTTTCTTAGGATTAGAACATTTGCATTAGACGATTATAGTCTTGCTTCTTTACTTTTCCATTGGAACTCTTTCTTGCAATGATCCCTTTTGGGAGTTTTTAAATTCATTTATCAATAAAATCTGTTTCTCTTCTAAAAAAAAGAACATTTGCATCAGAGACTGATTGTAGTTTTTATTTTTGCCTCCAATCAGGACCATTGTCTCAGTCGAAGAATACAAAATTTGAGTCAGTTTTCAAAATTTCAAGAAAGACATTCAGCTATATCTGTTCTCTTGTTAAGGAAGCTATGATGGCCAAAACTTCAAATTATACTGACTTAAACGGCAAGCCTTTGTCTCTAAATGACCAAGTTGCTGTTGCTCTTAGGCGGCTTAGCTCCGGTGAATCATTGTCGAATATCGGTGATTTATTTGGAATGAATCAATCATCAGTTTCCCAAATAACTTGGCGTTTCGTGGAGGCGATGGAAGAGAAAGGGCTCCACCATCTCTCGTGGCCTTCTACAGAGGAAGATATGGATCAGATCAAGTCCAAGTTTAAGAAAATCAGAGGCCTTCCTAATTGTTGCGGTGTAATCGAAACGACACACATTATGATGACTTTGCCAACGGCAGAATCTGCAAACGGCGTCTGGCTTGATCGTGAGAAAAACTGCAGCATGATCTTGCAAGTGATTGTAGACCCGGAAATGAGATTCTGTGACATCATCACAGGTTGGCCAGGAAGTTTGAGCGATGCTCTTGTGCTCCAAAGCTCGGGATTTTTCAAACTTTCCCAAGATGGCGAGCGGTTAAGCGGCAAGAAGATGAAGCTCTCAGAAAGTTCAGAAGTAGGAGAGTATATTATAGGAGACTCTGGTTTCCCCCTGTTGCCATGGCTACTAACTCCCTATCAAGGGAAAGGCCTTTCGGATTATCAGACCGAGTTCAATAAGCGGCATTTTGCCACCAGGTTGGTGGCTCAAAGGGCATTGACAAGGTTGAAAGAGATGTGGAAGATCATTAAAGGGGTAATGTGGAAGCCTGACAAACACAGGCTACCAAGGATCATTCTTGTTTGCTGCTTACTTCACAATATAGTGATCGATATGGAGGACGAGGTGCAAGACGAAATGCCCTTGTCTCATCATCACGATCCAAGTTACCGACAACAAAGTTGTGAATTCGTCGACAATACGGCTTCCATCGCGAGGGAGAAGCTCTCCATGTACTTATCTGGAAAGCTACCACCCTAA

mRNA sequence

ATGGGACCCATTAGAGGGTTCAAGAGGAAGAAGAAGACTGAGAAGAAGGTTGACCAAAATGTCTTGGCTGCTTCTTCACTAACGTCTCAGCCCCAGCCCTTGGATTGGTGGGATGAGTTCTCTCAGAGGATTACTGGACCATTGTCTCAGTCGAAGAATACAAAATTTGAGTCAGTTTTCAAAATTTCAAGAAAGACATTCAGCTATATCTGTTCTCTTGTTAAGGAAGCTATGATGGCCAAAACTTCAAATTATACTGACTTAAACGGCAAGCCTTTGTCTCTAAATGACCAAGTTGCTGTTGCTCTTAGGCGGCTTAGCTCCGGTGAATCATTGTCGAATATCGGTGATTTATTTGGAATGAATCAATCATCAGTTTCCCAAATAACTTGGCGTTTCGTGGAGGCGATGGAAGAGAAAGGGCTCCACCATCTCTCGTGGCCTTCTACAGAGGAAGATATGGATCAGATCAAGTCCAAGTTTAAGAAAATCAGAGGCCTTCCTAATTGTTGCGGTGTAATCGAAACGACACACATTATGATGACTTTGCCAACGGCAGAATCTGCAAACGGCGTCTGGCTTGATCGTGAGAAAAACTGCAGCATGATCTTGCAAGTGATTGTAGACCCGGAAATGAGATTCTGTGACATCATCACAGGTTGGCCAGGAAGTTTGAGCGATGCTCTTGTGCTCCAAAGCTCGGGATTTTTCAAACTTTCCCAAGATGGCGAGCGGTTAAGCGGCAAGAAGATGAAGCTCTCAGAAAGTTCAGAAGTAGGAGAGTATATTATAGGAGACTCTGGTTTCCCCCTGTTGCCATGGCTACTAACTCCCTATCAAGGGAAAGGCCTTTCGGATTATCAGACCGAGTTCAATAAGCGGCATTTTGCCACCAGGTTGGTGGCTCAAAGGGCATTGACAAGGTTGAAAGAGATGTGGAAGATCATTAAAGGGGTAATGTGGAAGCCTGACAAACACAGGCTACCAAGGATCATTCTTGTTTGCTGCTTACTTCACAATATAGTGATCGATATGGAGGACGAGGTGCAAGACGAAATGCCCTTGTCTCATCATCACGATCCAAGTTACCGACAACAAAGTTGTGAATTCGTCGACAATACGGCTTCCATCGCGAGGGAGAAGCTCTCCATGTACTTATCTGGAAAGCTACCACCCTAA

Coding sequence (CDS)

ATGGGACCCATTAGAGGGTTCAAGAGGAAGAAGAAGACTGAGAAGAAGGTTGACCAAAATGTCTTGGCTGCTTCTTCACTAACGTCTCAGCCCCAGCCCTTGGATTGGTGGGATGAGTTCTCTCAGAGGATTACTGGACCATTGTCTCAGTCGAAGAATACAAAATTTGAGTCAGTTTTCAAAATTTCAAGAAAGACATTCAGCTATATCTGTTCTCTTGTTAAGGAAGCTATGATGGCCAAAACTTCAAATTATACTGACTTAAACGGCAAGCCTTTGTCTCTAAATGACCAAGTTGCTGTTGCTCTTAGGCGGCTTAGCTCCGGTGAATCATTGTCGAATATCGGTGATTTATTTGGAATGAATCAATCATCAGTTTCCCAAATAACTTGGCGTTTCGTGGAGGCGATGGAAGAGAAAGGGCTCCACCATCTCTCGTGGCCTTCTACAGAGGAAGATATGGATCAGATCAAGTCCAAGTTTAAGAAAATCAGAGGCCTTCCTAATTGTTGCGGTGTAATCGAAACGACACACATTATGATGACTTTGCCAACGGCAGAATCTGCAAACGGCGTCTGGCTTGATCGTGAGAAAAACTGCAGCATGATCTTGCAAGTGATTGTAGACCCGGAAATGAGATTCTGTGACATCATCACAGGTTGGCCAGGAAGTTTGAGCGATGCTCTTGTGCTCCAAAGCTCGGGATTTTTCAAACTTTCCCAAGATGGCGAGCGGTTAAGCGGCAAGAAGATGAAGCTCTCAGAAAGTTCAGAAGTAGGAGAGTATATTATAGGAGACTCTGGTTTCCCCCTGTTGCCATGGCTACTAACTCCCTATCAAGGGAAAGGCCTTTCGGATTATCAGACCGAGTTCAATAAGCGGCATTTTGCCACCAGGTTGGTGGCTCAAAGGGCATTGACAAGGTTGAAAGAGATGTGGAAGATCATTAAAGGGGTAATGTGGAAGCCTGACAAACACAGGCTACCAAGGATCATTCTTGTTTGCTGCTTACTTCACAATATAGTGATCGATATGGAGGACGAGGTGCAAGACGAAATGCCCTTGTCTCATCATCACGATCCAAGTTACCGACAACAAAGTTGTGAATTCGTCGACAATACGGCTTCCATCGCGAGGGAGAAGCTCTCCATGTACTTATCTGGAAAGCTACCACCCTAA

Protein sequence

MGPIRGFKRKKKTEKKVDQNVLAASSLTSQPQPLDWWDEFSQRITGPLSQSKNTKFESVFKISRKTFSYICSLVKEAMMAKTSNYTDLNGKPLSLNDQVAVALRRLSSGESLSNIGDLFGMNQSSVSQITWRFVEAMEEKGLHHLSWPSTEEDMDQIKSKFKKIRGLPNCCGVIETTHIMMTLPTAESANGVWLDREKNCSMILQVIVDPEMRFCDIITGWPGSLSDALVLQSSGFFKLSQDGERLSGKKMKLSESSEVGEYIIGDSGFPLLPWLLTPYQGKGLSDYQTEFNKRHFATRLVAQRALTRLKEMWKIIKGVMWKPDKHRLPRIILVCCLLHNIVIDMEDEVQDEMPLSHHHDPSYRQQSCEFVDNTASIAREKLSMYLSGKLPP
Homology
BLAST of Lag0040755 vs. NCBI nr
Match: XP_038891834.1 (protein ALP1-like [Benincasa hispida])

HSP 1 Score: 774.2 bits (1998), Expect = 5.2e-220
Identity = 377/392 (96.17%), Postives = 384/392 (97.96%), Query Frame = 0

Query: 1   MGPIRGFKRKKKTEKKVDQNVLAASSLTSQPQPLDWWDEFSQRITGPLSQSKNTKFESVF 60
           MGPIRGFKRKKK EKKVDQNV AA+SL+SQ QPLDWWDEFSQRITGPLSQSKNTKFESVF
Sbjct: 134 MGPIRGFKRKKKVEKKVDQNVFAAASLSSQLQPLDWWDEFSQRITGPLSQSKNTKFESVF 193

Query: 61  KISRKTFSYICSLVKEAMMAKTSNYTDLNGKPLSLNDQVAVALRRLSSGESLSNIGDLFG 120
           KISRKTFSYICSLVKE MMAKTSN+TDLNGKPLSLNDQVAVALRRL SGESLSNIG+ FG
Sbjct: 194 KISRKTFSYICSLVKEVMMAKTSNFTDLNGKPLSLNDQVAVALRRLCSGESLSNIGESFG 253

Query: 121 MNQSSVSQITWRFVEAMEEKGLHHLSWPSTEEDMDQIKSKFKKIRGLPNCCGVIETTHIM 180
           MNQSSVSQITWRFVEAMEEKGLHHLSWPSTEEDMDQIKSKFKKIRGLPNCCGVIETTHIM
Sbjct: 254 MNQSSVSQITWRFVEAMEEKGLHHLSWPSTEEDMDQIKSKFKKIRGLPNCCGVIETTHIM 313

Query: 181 MTLPTAESANGVWLDREKNCSMILQVIVDPEMRFCDIITGWPGSLSDALVLQSSGFFKLS 240
           MTLPT ESANG+WLDREKNCSMILQVIVDPEMRFCDIITGWPGSLSDALVLQSSGFFKLS
Sbjct: 314 MTLPTTESANGIWLDREKNCSMILQVIVDPEMRFCDIITGWPGSLSDALVLQSSGFFKLS 373

Query: 241 QDGERLSGKKMKLSESSEVGEYIIGDSGFPLLPWLLTPYQGKGLSDYQTEFNKRHFATRL 300
           QD ERL+GKKMKLSESSE+GEYIIGDSGFPLLPWLLTPYQGKGLSDYQTEFNKRHFATRL
Sbjct: 374 QDSERLNGKKMKLSESSELGEYIIGDSGFPLLPWLLTPYQGKGLSDYQTEFNKRHFATRL 433

Query: 301 VAQRALTRLKEMWKIIKGVMWKPDKHRLPRIILVCCLLHNIVIDMEDEVQDEMPLSHHHD 360
           VAQRALTRLKEMWKIIKGVMWKPDKHRLPRIILVCCLLHNIVIDMEDEVQDEMPLSHHHD
Sbjct: 434 VAQRALTRLKEMWKIIKGVMWKPDKHRLPRIILVCCLLHNIVIDMEDEVQDEMPLSHHHD 493

Query: 361 PSYRQQSCEFVDNTASIAREKLSMYLSGKLPP 393
           PSYRQQSCEFVDNTASIAREKLSMYLSGKLPP
Sbjct: 494 PSYRQQSCEFVDNTASIAREKLSMYLSGKLPP 525

BLAST of Lag0040755 vs. NCBI nr
Match: XP_004147700.1 (protein ALP1-like [Cucumis sativus] >KGN50531.1 hypothetical protein Csa_000507 [Cucumis sativus])

HSP 1 Score: 767.3 bits (1980), Expect = 6.4e-218
Identity = 371/392 (94.64%), Postives = 384/392 (97.96%), Query Frame = 0

Query: 1   MGPIRGFKRKKKTEKKVDQNVLAASSLTSQPQPLDWWDEFSQRITGPLSQSKNTKFESVF 60
           MGPIRGFKRKKK EKKVDQNV A++SL+SQ QPLDWWDEFSQRITGPLSQSKNTKFESVF
Sbjct: 1   MGPIRGFKRKKKVEKKVDQNVFASASLSSQLQPLDWWDEFSQRITGPLSQSKNTKFESVF 60

Query: 61  KISRKTFSYICSLVKEAMMAKTSNYTDLNGKPLSLNDQVAVALRRLSSGESLSNIGDLFG 120
           KISRKTFSYICSLVKE MMAKTS++TDLNGKPLSLNDQVAVALRRL SGESLSNIGD FG
Sbjct: 61  KISRKTFSYICSLVKEVMMAKTSSFTDLNGKPLSLNDQVAVALRRLCSGESLSNIGDSFG 120

Query: 121 MNQSSVSQITWRFVEAMEEKGLHHLSWPSTEEDMDQIKSKFKKIRGLPNCCGVIETTHIM 180
           +NQSSVSQITWRFVEAMEEKGLHHLSWPSTEEDMD+IKSKFKKIRGLPNCCGV+ETTHIM
Sbjct: 121 LNQSSVSQITWRFVEAMEEKGLHHLSWPSTEEDMDKIKSKFKKIRGLPNCCGVVETTHIM 180

Query: 181 MTLPTAESANGVWLDREKNCSMILQVIVDPEMRFCDIITGWPGSLSDALVLQSSGFFKLS 240
           MTLPT+ESANG+WLDREKNCSMILQVIVDPEMRFCDIITGWPGSLSDALVLQSSGFFKLS
Sbjct: 181 MTLPTSESANGIWLDREKNCSMILQVIVDPEMRFCDIITGWPGSLSDALVLQSSGFFKLS 240

Query: 241 QDGERLSGKKMKLSESSEVGEYIIGDSGFPLLPWLLTPYQGKGLSDYQTEFNKRHFATRL 300
           QDGERL+GKKMKLSESSE+GEYIIGDSGFPLLPWLLTPYQGKGL DYQ EFNKRHFATRL
Sbjct: 241 QDGERLNGKKMKLSESSELGEYIIGDSGFPLLPWLLTPYQGKGLPDYQAEFNKRHFATRL 300

Query: 301 VAQRALTRLKEMWKIIKGVMWKPDKHRLPRIILVCCLLHNIVIDMEDEVQDEMPLSHHHD 360
           VAQRALTRLKEMWKIIKGVMWKPDKHRLPRIILVCCLLHNIVIDMEDEVQDEMPLSHHHD
Sbjct: 301 VAQRALTRLKEMWKIIKGVMWKPDKHRLPRIILVCCLLHNIVIDMEDEVQDEMPLSHHHD 360

Query: 361 PSYRQQSCEFVDNTASIAREKLSMYLSGKLPP 393
           PSYRQQSCEFVDNTASI+REKLSMYLSGKLPP
Sbjct: 361 PSYRQQSCEFVDNTASISREKLSMYLSGKLPP 392

BLAST of Lag0040755 vs. NCBI nr
Match: XP_008461643.1 (PREDICTED: putative nuclease HARBI1 [Cucumis melo])

HSP 1 Score: 766.5 bits (1978), Expect = 1.1e-217
Identity = 371/392 (94.64%), Postives = 384/392 (97.96%), Query Frame = 0

Query: 1   MGPIRGFKRKKKTEKKVDQNVLAASSLTSQPQPLDWWDEFSQRITGPLSQSKNTKFESVF 60
           MGPIRGFKRKKK EKKVDQNV A++SL+SQ QPLDWWDEFSQRITGPLSQSKNTKFESVF
Sbjct: 1   MGPIRGFKRKKKVEKKVDQNVFASASLSSQLQPLDWWDEFSQRITGPLSQSKNTKFESVF 60

Query: 61  KISRKTFSYICSLVKEAMMAKTSNYTDLNGKPLSLNDQVAVALRRLSSGESLSNIGDLFG 120
           KISRKTFSYICSLVKE MMAKTS++TDLNGKPLSLNDQVAVALRRL SGESLSNIGD FG
Sbjct: 61  KISRKTFSYICSLVKEVMMAKTSSFTDLNGKPLSLNDQVAVALRRLCSGESLSNIGDSFG 120

Query: 121 MNQSSVSQITWRFVEAMEEKGLHHLSWPSTEEDMDQIKSKFKKIRGLPNCCGVIETTHIM 180
           +NQSSVSQITWRFVEAMEEKGLHHLSWPSTEEDMD+IKSKFKKIRGLPNCCGVIETTHIM
Sbjct: 121 LNQSSVSQITWRFVEAMEEKGLHHLSWPSTEEDMDKIKSKFKKIRGLPNCCGVIETTHIM 180

Query: 181 MTLPTAESANGVWLDREKNCSMILQVIVDPEMRFCDIITGWPGSLSDALVLQSSGFFKLS 240
           MTLPT+ESANG+WLDREKNCSMILQVIVDPEMRFCDIITGWPGSLSD+LVLQSSGFFKLS
Sbjct: 181 MTLPTSESANGIWLDREKNCSMILQVIVDPEMRFCDIITGWPGSLSDSLVLQSSGFFKLS 240

Query: 241 QDGERLSGKKMKLSESSEVGEYIIGDSGFPLLPWLLTPYQGKGLSDYQTEFNKRHFATRL 300
           QDGERL+GKKM+LSESSE+GEYIIGDSGFPLLPWLLTPYQGKGL DYQ EFNKRHFATRL
Sbjct: 241 QDGERLNGKKMRLSESSELGEYIIGDSGFPLLPWLLTPYQGKGLPDYQAEFNKRHFATRL 300

Query: 301 VAQRALTRLKEMWKIIKGVMWKPDKHRLPRIILVCCLLHNIVIDMEDEVQDEMPLSHHHD 360
           VAQRALTRLKEMWKIIKGVMWKPDKHRLPRIILVCCLLHNIVIDMEDEVQDEMPLSHHHD
Sbjct: 301 VAQRALTRLKEMWKIIKGVMWKPDKHRLPRIILVCCLLHNIVIDMEDEVQDEMPLSHHHD 360

Query: 361 PSYRQQSCEFVDNTASIAREKLSMYLSGKLPP 393
           PSYRQQSCEFVDNTASIAREKLSMYLSGKLPP
Sbjct: 361 PSYRQQSCEFVDNTASIAREKLSMYLSGKLPP 392

BLAST of Lag0040755 vs. NCBI nr
Match: XP_022941714.1 (protein ALP1-like [Cucurbita moschata])

HSP 1 Score: 761.9 bits (1966), Expect = 2.7e-216
Identity = 372/392 (94.90%), Postives = 382/392 (97.45%), Query Frame = 0

Query: 1   MGPIRGFKRKKKTEKKVDQNVLAASSLTSQPQPLDWWDEFSQRITGPLSQSKNTKFESVF 60
           MGPIRGFKRKK   KKVDQNVL  SSLTSQPQPLDWWDEFSQRITGPLS+SKNT FESVF
Sbjct: 1   MGPIRGFKRKK---KKVDQNVLVPSSLTSQPQPLDWWDEFSQRITGPLSESKNTNFESVF 60

Query: 61  KISRKTFSYICSLVKEAMMAKTSNYTDLNGKPLSLNDQVAVALRRLSSGESLSNIGDLFG 120
           KISRKTFSYI SLVKEAMMAKTSN+TDLNGKPLS+NDQVAVALRRLSSGESLSNIGD FG
Sbjct: 61  KISRKTFSYISSLVKEAMMAKTSNFTDLNGKPLSINDQVAVALRRLSSGESLSNIGDSFG 120

Query: 121 MNQSSVSQITWRFVEAMEEKGLHHLSWPSTEEDMDQIKSKFKKIRGLPNCCGVIETTHIM 180
           MNQSSVSQITWRFVEAMEEKGLHHLSWPSTEE MD+IKSKFKKI+GLPNCCGVIETTHIM
Sbjct: 121 MNQSSVSQITWRFVEAMEEKGLHHLSWPSTEEGMDEIKSKFKKIKGLPNCCGVIETTHIM 180

Query: 181 MTLPTAESANGVWLDREKNCSMILQVIVDPEMRFCDIITGWPGSLSDALVLQSSGFFKLS 240
           MTLPT ESA+GVWLDREKNCSM+LQVIVDPEMRFCDIITGWPGSLSDALVLQSSGFFKLS
Sbjct: 181 MTLPTTESAHGVWLDREKNCSMLLQVIVDPEMRFCDIITGWPGSLSDALVLQSSGFFKLS 240

Query: 241 QDGERLSGKKMKLSESSEVGEYIIGDSGFPLLPWLLTPYQGKGLSDYQTEFNKRHFATRL 300
           QDGERL+GKKMKLSESSEVGEYIIGDSGFPLLPWLLTPYQGKGLSDYQTEFNKRHFATRL
Sbjct: 241 QDGERLNGKKMKLSESSEVGEYIIGDSGFPLLPWLLTPYQGKGLSDYQTEFNKRHFATRL 300

Query: 301 VAQRALTRLKEMWKIIKGVMWKPDKHRLPRIILVCCLLHNIVIDMEDEVQDEMPLSHHHD 360
           VAQRALTRLKEMWKIIKGVMWKPDKHRLPRI+LVCCLLHNIVIDMEDEVQDEMPLSHHHD
Sbjct: 301 VAQRALTRLKEMWKIIKGVMWKPDKHRLPRIVLVCCLLHNIVIDMEDEVQDEMPLSHHHD 360

Query: 361 PSYRQQSCEFVDNTASIAREKLSMYLSGKLPP 393
           PSYRQQSCEFVDNTAS+AREKLSMYLSGKLPP
Sbjct: 361 PSYRQQSCEFVDNTASMAREKLSMYLSGKLPP 389

BLAST of Lag0040755 vs. NCBI nr
Match: XP_022138922.1 (protein ALP1-like [Momordica charantia])

HSP 1 Score: 761.1 bits (1964), Expect = 4.6e-216
Identity = 371/393 (94.40%), Postives = 383/393 (97.46%), Query Frame = 0

Query: 1   MGPIRGFKRKKKTEKKVDQNVLAASSLTSQPQPLDWWDEFSQRITGPLSQSKN-TKFESV 60
           MGPIRGFKRKKK EKKVDQNVLAA+SL+SQPQPLDWWD+FSQRITGPLSQSKN TKFESV
Sbjct: 1   MGPIRGFKRKKKAEKKVDQNVLAAASLSSQPQPLDWWDDFSQRITGPLSQSKNPTKFESV 60

Query: 61  FKISRKTFSYICSLVKEAMMAKTSNYTDLNGKPLSLNDQVAVALRRLSSGESLSNIGDLF 120
           FKISRKTFSYICSLVKEAMMAKTSN+TDLNGKPLS+NDQVAVALRRLSSGESLS IGD F
Sbjct: 61  FKISRKTFSYICSLVKEAMMAKTSNFTDLNGKPLSVNDQVAVALRRLSSGESLSIIGDSF 120

Query: 121 GMNQSSVSQITWRFVEAMEEKGLHHLSWPSTEEDMDQIKSKFKKIRGLPNCCGVIETTHI 180
           GMNQSSVSQITWRFVEAMEEKGLHHLSWPSTEEDMDQIKSKFKKI+GLPNCCGVIETTHI
Sbjct: 121 GMNQSSVSQITWRFVEAMEEKGLHHLSWPSTEEDMDQIKSKFKKIKGLPNCCGVIETTHI 180

Query: 181 MMTLPTAESANGVWLDREKNCSMILQVIVDPEMRFCDIITGWPGSLSDALVLQSSGFFKL 240
           MMTLPTAES NGVWLDREKNCSMILQVIVDPEMRFCDI+ GWPGSLSDALVLQSSGFFKL
Sbjct: 181 MMTLPTAESXNGVWLDREKNCSMILQVIVDPEMRFCDIMAGWPGSLSDALVLQSSGFFKL 240

Query: 241 SQDGERLSGKKMKLSESSEVGEYIIGDSGFPLLPWLLTPYQGKGLSDYQTEFNKRHFATR 300
           SQDGERL+GK MKLSESSE+GEYIIGDSGFPLLPWLLTPYQGKGLSDYQTEFNKRH+ATR
Sbjct: 241 SQDGERLNGKNMKLSESSELGEYIIGDSGFPLLPWLLTPYQGKGLSDYQTEFNKRHYATR 300

Query: 301 LVAQRALTRLKEMWKIIKGVMWKPDKHRLPRIILVCCLLHNIVIDMEDEVQDEMPLSHHH 360
           LVAQRALTRLKEMWKIIKGVMWKPDKHRLPRIILVCCLLHNIVIDMEDEVQDEMPLSHHH
Sbjct: 301 LVAQRALTRLKEMWKIIKGVMWKPDKHRLPRIILVCCLLHNIVIDMEDEVQDEMPLSHHH 360

Query: 361 DPSYRQQSCEFVDNTASIAREKLSMYLSGKLPP 393
           D  YRQQSC+FVDNTAS+ REKLSMYLSGKLPP
Sbjct: 361 DSGYRQQSCKFVDNTASVVREKLSMYLSGKLPP 393

BLAST of Lag0040755 vs. ExPASy Swiss-Prot
Match: Q9M2U3 (Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 2.4e-143
Identity = 251/407 (61.67%), Postives = 306/407 (75.18%), Query Frame = 0

Query: 1   MGPIRGFKRKKKTEKKVDQNVLAASSLT------------------SQPQPLDWWDEFSQ 60
           MGPI+  K+KK+ EKKVD+NVL A++                    S  Q LDWWD FS+
Sbjct: 1   MGPIKTIKKKKRAEKKVDRNVLLAATAAATSASAAAALNNNDDDDDSSSQSLDWWDGFSR 60

Query: 61  RITGPLSQSKNTKFESVFKISRKTFSYICSLVKEAMMAKTSNYTDLNGKPLSLNDQVAVA 120
           RI G  +  K   FESVFKISRKTF YICSLVK    AK +N++D NG PLSLND+VAVA
Sbjct: 61  RIYGGSTDPKT--FESVFKISRKTFDYICSLVKADFTAKPANFSDSNGNPLSLNDRVAVA 120

Query: 121 LRRLSSGESLSNIGDLFGMNQSSVSQITWRFVEAMEEKGLHHLSWPSTEEDMDQIKSKFK 180
           LRRL SGESLS IG+ FGMNQS+VSQITWRFVE+MEE+ +HHLSWPS    +D+IKSKF+
Sbjct: 121 LRRLGSGESLSVIGETFGMNQSTVSQITWRFVESMEERAIHHLSWPS---KLDEIKSKFE 180

Query: 181 KIRGLPNCCGVIETTHIMMTLPTAESANGVWLDREKNCSMILQVIVDPEMRFCDIITGWP 240
           KI GLPNCCG I+ THI+M LP  E +N VWLD EKN SM LQ +VDP+MRF D+I GWP
Sbjct: 181 KISGLPNCCGAIDITHIVMNLPAVEPSNKVWLDGEKNFSMTLQAVVDPDMRFLDVIAGWP 240

Query: 241 GSLSDALVLQSSGFFKLSQDGERLSGKKMKLSESSEVGEYIIGDSGFPLLPWLLTPYQGK 300
           GSL+D +VL++SGF+KL + G+RL+G+K+ LSE +E+ EYI+GDSGFPLLPWLLTPYQGK
Sbjct: 241 GSLNDDVVLKNSGFYKLVEKGKRLNGEKLPLSERTELREYIVGDSGFPLLPWLLTPYQGK 300

Query: 301 GLSDYQTEFNKRHFATRLVAQRALTRLKEMWKIIKGVMWKPDKHRLPRIILVCCLLHNIV 360
             S  QTEFNKRH      AQ AL++LK+ W+II GVMW PD++RLPRII VCCLLHNI+
Sbjct: 301 PTSLPQTEFNKRHSEATKAAQMALSKLKDRWRIINGVMWMPDRNRLPRIIFVCCLLHNII 360

Query: 361 IDMEDEVQDEMPLSHHHDPSYRQQSCEFVDNTASIAREKLSMYLSGK 390
           IDMED+  D+ PLS  HD +YRQ+SC+  D  +S+ R++LS  L GK
Sbjct: 361 IDMEDQTLDDQPLSQQHDMNYRQRSCKLADEASSVLRDELSDQLCGK 402

BLAST of Lag0040755 vs. ExPASy Swiss-Prot
Match: Q94K49 (Protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=ALP1 PE=1 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 4.4e-97
Identity = 171/380 (45.00%), Postives = 256/380 (67.37%), Query Frame = 0

Query: 9   RKKKTEKKVDQNVLAASSLTSQPQPLDWWDEFSQRITGP-LSQSKNTKFESVFKISRKTF 68
           + KK  K  ++  + A  L  +    DWWD F  R + P +   ++  F+  F+ S+ TF
Sbjct: 17  KAKKLAKNKEKKRVNAVPLDPEAIDCDWWDTFWLRNSSPSVPSDEDYAFKHFFRASKTTF 76

Query: 69  SYICSLVKEAMMAK-TSNYTDLNGKPLSLNDQVAVALRRLSSGESLSNIGDLFGMNQSSV 128
           SYICSLV+E ++++  S   ++ G+ LS+  QVA+ALRRL+SG+S  ++G  FG+ QS+V
Sbjct: 77  SYICSLVREDLISRPPSGLINIEGRLLSVEKQVAIALRRLASGDSQVSVGAAFGVGQSTV 136

Query: 129 SQITWRFVEAMEEKGLHHLSWPSTEEDMDQIKSKFKKIRGLPNCCGVIETTHIMMTLPTA 188
           SQ+TWRF+EA+EE+  HHL WP ++  +++IKSKF+++ GLPNCCG I+TTHI+MTLP  
Sbjct: 137 SQVTWRFIEALEERAKHHLRWPDSDR-IEEIKSKFEEMYGLPNCCGAIDTTHIIMTLPAV 196

Query: 189 ESANGVWLDREKNCSMILQVIVDPEMRFCDIITGWPGSLSDALVLQSSGFFKLSQDGERL 248
           ++++  W D+EKN SM LQ + D EMRF +++TGWPG ++ + +L+ SGFFKL ++ + L
Sbjct: 197 QASDD-WCDQEKNYSMFLQGVFDHEMRFLNMVTGWPGGMTVSKLLKFSGFFKLCENAQIL 256

Query: 249 SGKKMKLSESSEVGEYIIGDSGFPLLPWLLTPYQGKGLSDYQTEFNKRHFATRLVAQRAL 308
            G    LS+ +++ EY++G   +PLLPWL+TP+     SD    FN+RH   R VA  A 
Sbjct: 257 DGNPKTLSQGAQIREYVVGGISYPLLPWLITPHDSDHPSDSMVAFNERHEKVRSVAATAF 316

Query: 309 TRLKEMWKIIKGVMWKPDKHRLPRIILVCCLLHNIVIDMEDEVQDEMPLSHHHDPSYRQQ 368
            +LK  W+I+  VMW+PD+ +LP IILVCCLLHNI+ID  D +Q+++PLS HHD  Y  +
Sbjct: 317 QQLKGSWRILSKVMWRPDRRKLPSIILVCCLLHNIIIDCGDYLQEDVPLSGHHDSGYADR 376

Query: 369 SCEFVDNTASIAREKLSMYL 387
            C+  +   S  R  L+ +L
Sbjct: 377 YCKQTEPLGSELRGCLTEHL 394

BLAST of Lag0040755 vs. ExPASy Swiss-Prot
Match: Q6AZB8 (Putative nuclease HARBI1 OS=Danio rerio OX=7955 GN=harbi1 PE=2 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 2.8e-27
Identity = 78/289 (26.99%), Postives = 145/289 (50.17%), Query Frame = 0

Query: 58  SVFKISRKTFSYICSLVKEAMMAKTSNYTDLNGKPLSLNDQVAVALRRLSSGESLSNIGD 117
           + F   R+   Y+  L+K++++ +T        + +S + Q+  AL   +SG   S +GD
Sbjct: 37  NTFGFPREFIYYLVELLKDSLLRRTQ-----RSRAISPDVQILAALGFYTSGSFQSKMGD 96

Query: 118 LFGMNQSSVSQITWRFVEAMEEKGLHHLSWPSTEEDMDQIKSKFKKIRGLPNCCGVIETT 177
             G++Q+S+S+      +A+ EK    + +   E    Q K +F +I G+PN  GV++  
Sbjct: 97  AIGISQASMSRCVSNVTKALIEKAPEFIGFTRDEATKQQFKDEFYRIAGIPNVTGVVDCA 156

Query: 178 HIMMTLPTAESANGVWLDREKNCSMILQVIVDPEMRFCDIITGWPGSLSDALVLQSSGFF 237
           HI +  P A+ ++  +++++   S+  Q++ D         T WPGSL+D  V + S   
Sbjct: 157 HIAIKAPNADDSS--YVNKKGFHSINCQLVCDARGLLLSAETHWPGSLTDRAVFKQSNVA 216

Query: 238 KLSQDGERLSGKKMKLSESSEVGEYIIGDSGFPLLPWLLTPYQG-KGLSDYQTEFNKRHF 297
           KL ++            E+ + G +++GD+ +PL  WL+TP Q  +  +DY+  +N  H 
Sbjct: 217 KLFEE-----------QENDDEG-WLLGDNRYPLKKWLMTPVQSPESPADYR--YNLAHT 276

Query: 298 ATRLVAQRALTRLKEMWKIIKG----VMWKPDKHRLPRIILVCCLLHNI 342
            T  +  R    ++  ++ + G    + + P+K     II  CC+LHNI
Sbjct: 277 TTHEIVDRTFRAIQTRFRCLDGAKGYLQYSPEK--CSHIIQACCVLHNI 302

BLAST of Lag0040755 vs. ExPASy Swiss-Prot
Match: Q8BR93 (Putative nuclease HARBI1 OS=Mus musculus OX=10090 GN=Harbi1 PE=2 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 4.5e-25
Identity = 92/339 (27.14%), Postives = 153/339 (45.13%), Query Frame = 0

Query: 60  FKISRKTFSYICSL----------VKEAMMAKTSNYTDLNGKPLSLNDQVAVALRRLSSG 119
           FK+   T  Y+ S+          + E + A  S  T    + +S   Q+  AL   +SG
Sbjct: 25  FKLDDVTDEYLMSMYGFPRQFIYFLVELLGASLSRPTQ-RSRAISPETQILAALGFYTSG 84

Query: 120 ESLSNIGDLFGMNQSSVSQITWRFVEAMEEKGLHHLSWPSTEEDMDQIKSKFKKIRGLPN 179
              + +GD  G++Q+S+S+      EA+ E+    + +P  E  +  +K +F  + G+P 
Sbjct: 85  SFQTRMGDAIGISQASMSRCVANVTEALVERASQFIHFPVDEAAVQSLKDEFYGLAGMPG 144

Query: 180 CCGVIETTHIMMTLPTAESANGVWLDREKNCSMILQVIVDPEMRFCDIITGWPGSLSDAL 239
             GV +  H+ +  P AE  +  +++R+   S+   V+ D       + T WPGSL D  
Sbjct: 145 VIGVADCIHVAIKAPNAEDLS--YVNRKGLHSLNCLVVCDIRGALMTVETSWPGSLQDCA 204

Query: 240 VLQSSGFFKLSQDGERLSGKKMKLSESSEVGEYIIGDSGFPLLPWLLTPYQ-GKGLSDYQ 299
           VLQ S      + G                  +++GDS F L  WLLTP    +  ++Y+
Sbjct: 205 VLQRSSLTSQFETG-------------MPKDSWLLGDSSFFLRSWLLTPLPIPETAAEYR 264

Query: 300 TEFNKRHFATRLVAQRALTRLKEMWKIIKG----VMWKPDKHRLPRIILVCCLLHNIVID 359
             +N+ H AT  V +R L  L   ++ + G    + + P+K     IIL CC+LHNI +D
Sbjct: 265 --YNRAHSATHSVIERTLQTLCCRFRCLDGSKGALQYSPEK--CSHIILACCVLHNISLD 324

Query: 360 MEDEV-QDEMPLSHHHDPSYRQQSCEFVDNTASIAREKL 383
              +V    +P      P    +  E +D  A   R++L
Sbjct: 325 HGMDVWSSPVPGPIDQPPEGEDEHMESLDLEADRIRQEL 343

BLAST of Lag0040755 vs. ExPASy Swiss-Prot
Match: Q96MB7 (Putative nuclease HARBI1 OS=Homo sapiens OX=9606 GN=HARBI1 PE=1 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 1.0e-24
Identity = 91/345 (26.38%), Postives = 156/345 (45.22%), Query Frame = 0

Query: 60  FKISRKTFSYICSL----------VKEAMMAKTSNYTDLNGKPLSLNDQVAVALRRLSSG 119
           FK+   T  Y+ S+          + E + A  S  T    + +S   QV  AL   +SG
Sbjct: 25  FKLDDVTDEYLMSMYGFPRQFIYYLVELLGANLSRPTQ-RSRAISPETQVLAALGFYTSG 84

Query: 120 ESLSNIGDLFGMNQSSVSQITWRFVEAMEEKGLHHLSWPSTEEDMDQIKSKFKKIRGLPN 179
              + +GD  G++Q+S+S+      EA+ E+    + +P+ E  +  +K +F  + G+P 
Sbjct: 85  SFQTRMGDAIGISQASMSRCVANVTEALVERASQFIRFPADEASIQALKDEFYGLAGMPG 144

Query: 180 CCGVIETTHIMMTLPTAESANGVWLDREKNCSMILQVIVDPEMRFCDIITGWPGSLSDAL 239
             GV++  H+ +  P AE  +  +++R+   S+   ++ D       + T WPGSL D  
Sbjct: 145 VMGVVDCIHVAIKAPNAEDLS--YVNRKGLHSLNCLMVCDIRGTLMTVETNWPGSLQDCA 204

Query: 240 VLQSSGFFKLSQDGERLSGKKMKLSESSEVG----EYIIGDSGFPLLPWLLTP-YQGKGL 299
           VLQ S                  LS   E G     +++GDS F L  WL+TP +  +  
Sbjct: 205 VLQQS-----------------SLSSQFEAGMHKDSWLLGDSSFFLRTWLMTPLHIPETP 264

Query: 300 SDYQTEFNKRHFATRLVAQRALTRLKEMWKIIKG----VMWKPDKHRLPRIILVCCLLHN 359
           ++Y+  +N  H AT  V ++    L   ++ + G    + + P+K     IIL CC+LHN
Sbjct: 265 AEYR--YNMAHSATHSVIEKTFRTLCSRFRCLDGSKGALQYSPEKS--SHIILACCVLHN 324

Query: 360 IVIDMEDEV-QDEMPLSHHHDPSYRQQSCEFVDNTASIAREKLSM 385
           I ++   +V    M       P    +  E +D  A   R++L +
Sbjct: 325 ISLEHGMDVWSSPMTGPMEQPPEEEYEHMESLDLEADRIRQELML 345

BLAST of Lag0040755 vs. ExPASy TrEMBL
Match: A0A0A0KS64 (DDE Tnp4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G180900 PE=3 SV=1)

HSP 1 Score: 767.3 bits (1980), Expect = 3.1e-218
Identity = 371/392 (94.64%), Postives = 384/392 (97.96%), Query Frame = 0

Query: 1   MGPIRGFKRKKKTEKKVDQNVLAASSLTSQPQPLDWWDEFSQRITGPLSQSKNTKFESVF 60
           MGPIRGFKRKKK EKKVDQNV A++SL+SQ QPLDWWDEFSQRITGPLSQSKNTKFESVF
Sbjct: 1   MGPIRGFKRKKKVEKKVDQNVFASASLSSQLQPLDWWDEFSQRITGPLSQSKNTKFESVF 60

Query: 61  KISRKTFSYICSLVKEAMMAKTSNYTDLNGKPLSLNDQVAVALRRLSSGESLSNIGDLFG 120
           KISRKTFSYICSLVKE MMAKTS++TDLNGKPLSLNDQVAVALRRL SGESLSNIGD FG
Sbjct: 61  KISRKTFSYICSLVKEVMMAKTSSFTDLNGKPLSLNDQVAVALRRLCSGESLSNIGDSFG 120

Query: 121 MNQSSVSQITWRFVEAMEEKGLHHLSWPSTEEDMDQIKSKFKKIRGLPNCCGVIETTHIM 180
           +NQSSVSQITWRFVEAMEEKGLHHLSWPSTEEDMD+IKSKFKKIRGLPNCCGV+ETTHIM
Sbjct: 121 LNQSSVSQITWRFVEAMEEKGLHHLSWPSTEEDMDKIKSKFKKIRGLPNCCGVVETTHIM 180

Query: 181 MTLPTAESANGVWLDREKNCSMILQVIVDPEMRFCDIITGWPGSLSDALVLQSSGFFKLS 240
           MTLPT+ESANG+WLDREKNCSMILQVIVDPEMRFCDIITGWPGSLSDALVLQSSGFFKLS
Sbjct: 181 MTLPTSESANGIWLDREKNCSMILQVIVDPEMRFCDIITGWPGSLSDALVLQSSGFFKLS 240

Query: 241 QDGERLSGKKMKLSESSEVGEYIIGDSGFPLLPWLLTPYQGKGLSDYQTEFNKRHFATRL 300
           QDGERL+GKKMKLSESSE+GEYIIGDSGFPLLPWLLTPYQGKGL DYQ EFNKRHFATRL
Sbjct: 241 QDGERLNGKKMKLSESSELGEYIIGDSGFPLLPWLLTPYQGKGLPDYQAEFNKRHFATRL 300

Query: 301 VAQRALTRLKEMWKIIKGVMWKPDKHRLPRIILVCCLLHNIVIDMEDEVQDEMPLSHHHD 360
           VAQRALTRLKEMWKIIKGVMWKPDKHRLPRIILVCCLLHNIVIDMEDEVQDEMPLSHHHD
Sbjct: 301 VAQRALTRLKEMWKIIKGVMWKPDKHRLPRIILVCCLLHNIVIDMEDEVQDEMPLSHHHD 360

Query: 361 PSYRQQSCEFVDNTASIAREKLSMYLSGKLPP 393
           PSYRQQSCEFVDNTASI+REKLSMYLSGKLPP
Sbjct: 361 PSYRQQSCEFVDNTASISREKLSMYLSGKLPP 392

BLAST of Lag0040755 vs. ExPASy TrEMBL
Match: A0A1S3CEZ1 (putative nuclease HARBI1 OS=Cucumis melo OX=3656 GN=LOC103500196 PE=3 SV=1)

HSP 1 Score: 766.5 bits (1978), Expect = 5.3e-218
Identity = 371/392 (94.64%), Postives = 384/392 (97.96%), Query Frame = 0

Query: 1   MGPIRGFKRKKKTEKKVDQNVLAASSLTSQPQPLDWWDEFSQRITGPLSQSKNTKFESVF 60
           MGPIRGFKRKKK EKKVDQNV A++SL+SQ QPLDWWDEFSQRITGPLSQSKNTKFESVF
Sbjct: 1   MGPIRGFKRKKKVEKKVDQNVFASASLSSQLQPLDWWDEFSQRITGPLSQSKNTKFESVF 60

Query: 61  KISRKTFSYICSLVKEAMMAKTSNYTDLNGKPLSLNDQVAVALRRLSSGESLSNIGDLFG 120
           KISRKTFSYICSLVKE MMAKTS++TDLNGKPLSLNDQVAVALRRL SGESLSNIGD FG
Sbjct: 61  KISRKTFSYICSLVKEVMMAKTSSFTDLNGKPLSLNDQVAVALRRLCSGESLSNIGDSFG 120

Query: 121 MNQSSVSQITWRFVEAMEEKGLHHLSWPSTEEDMDQIKSKFKKIRGLPNCCGVIETTHIM 180
           +NQSSVSQITWRFVEAMEEKGLHHLSWPSTEEDMD+IKSKFKKIRGLPNCCGVIETTHIM
Sbjct: 121 LNQSSVSQITWRFVEAMEEKGLHHLSWPSTEEDMDKIKSKFKKIRGLPNCCGVIETTHIM 180

Query: 181 MTLPTAESANGVWLDREKNCSMILQVIVDPEMRFCDIITGWPGSLSDALVLQSSGFFKLS 240
           MTLPT+ESANG+WLDREKNCSMILQVIVDPEMRFCDIITGWPGSLSD+LVLQSSGFFKLS
Sbjct: 181 MTLPTSESANGIWLDREKNCSMILQVIVDPEMRFCDIITGWPGSLSDSLVLQSSGFFKLS 240

Query: 241 QDGERLSGKKMKLSESSEVGEYIIGDSGFPLLPWLLTPYQGKGLSDYQTEFNKRHFATRL 300
           QDGERL+GKKM+LSESSE+GEYIIGDSGFPLLPWLLTPYQGKGL DYQ EFNKRHFATRL
Sbjct: 241 QDGERLNGKKMRLSESSELGEYIIGDSGFPLLPWLLTPYQGKGLPDYQAEFNKRHFATRL 300

Query: 301 VAQRALTRLKEMWKIIKGVMWKPDKHRLPRIILVCCLLHNIVIDMEDEVQDEMPLSHHHD 360
           VAQRALTRLKEMWKIIKGVMWKPDKHRLPRIILVCCLLHNIVIDMEDEVQDEMPLSHHHD
Sbjct: 301 VAQRALTRLKEMWKIIKGVMWKPDKHRLPRIILVCCLLHNIVIDMEDEVQDEMPLSHHHD 360

Query: 361 PSYRQQSCEFVDNTASIAREKLSMYLSGKLPP 393
           PSYRQQSCEFVDNTASIAREKLSMYLSGKLPP
Sbjct: 361 PSYRQQSCEFVDNTASIAREKLSMYLSGKLPP 392

BLAST of Lag0040755 vs. ExPASy TrEMBL
Match: A0A6J1FP85 (protein ALP1-like OS=Cucurbita moschata OX=3662 GN=LOC111446995 PE=3 SV=1)

HSP 1 Score: 761.9 bits (1966), Expect = 1.3e-216
Identity = 372/392 (94.90%), Postives = 382/392 (97.45%), Query Frame = 0

Query: 1   MGPIRGFKRKKKTEKKVDQNVLAASSLTSQPQPLDWWDEFSQRITGPLSQSKNTKFESVF 60
           MGPIRGFKRKK   KKVDQNVL  SSLTSQPQPLDWWDEFSQRITGPLS+SKNT FESVF
Sbjct: 1   MGPIRGFKRKK---KKVDQNVLVPSSLTSQPQPLDWWDEFSQRITGPLSESKNTNFESVF 60

Query: 61  KISRKTFSYICSLVKEAMMAKTSNYTDLNGKPLSLNDQVAVALRRLSSGESLSNIGDLFG 120
           KISRKTFSYI SLVKEAMMAKTSN+TDLNGKPLS+NDQVAVALRRLSSGESLSNIGD FG
Sbjct: 61  KISRKTFSYISSLVKEAMMAKTSNFTDLNGKPLSINDQVAVALRRLSSGESLSNIGDSFG 120

Query: 121 MNQSSVSQITWRFVEAMEEKGLHHLSWPSTEEDMDQIKSKFKKIRGLPNCCGVIETTHIM 180
           MNQSSVSQITWRFVEAMEEKGLHHLSWPSTEE MD+IKSKFKKI+GLPNCCGVIETTHIM
Sbjct: 121 MNQSSVSQITWRFVEAMEEKGLHHLSWPSTEEGMDEIKSKFKKIKGLPNCCGVIETTHIM 180

Query: 181 MTLPTAESANGVWLDREKNCSMILQVIVDPEMRFCDIITGWPGSLSDALVLQSSGFFKLS 240
           MTLPT ESA+GVWLDREKNCSM+LQVIVDPEMRFCDIITGWPGSLSDALVLQSSGFFKLS
Sbjct: 181 MTLPTTESAHGVWLDREKNCSMLLQVIVDPEMRFCDIITGWPGSLSDALVLQSSGFFKLS 240

Query: 241 QDGERLSGKKMKLSESSEVGEYIIGDSGFPLLPWLLTPYQGKGLSDYQTEFNKRHFATRL 300
           QDGERL+GKKMKLSESSEVGEYIIGDSGFPLLPWLLTPYQGKGLSDYQTEFNKRHFATRL
Sbjct: 241 QDGERLNGKKMKLSESSEVGEYIIGDSGFPLLPWLLTPYQGKGLSDYQTEFNKRHFATRL 300

Query: 301 VAQRALTRLKEMWKIIKGVMWKPDKHRLPRIILVCCLLHNIVIDMEDEVQDEMPLSHHHD 360
           VAQRALTRLKEMWKIIKGVMWKPDKHRLPRI+LVCCLLHNIVIDMEDEVQDEMPLSHHHD
Sbjct: 301 VAQRALTRLKEMWKIIKGVMWKPDKHRLPRIVLVCCLLHNIVIDMEDEVQDEMPLSHHHD 360

Query: 361 PSYRQQSCEFVDNTASIAREKLSMYLSGKLPP 393
           PSYRQQSCEFVDNTAS+AREKLSMYLSGKLPP
Sbjct: 361 PSYRQQSCEFVDNTASMAREKLSMYLSGKLPP 389

BLAST of Lag0040755 vs. ExPASy TrEMBL
Match: A0A6J1CCK2 (protein ALP1-like OS=Momordica charantia OX=3673 GN=LOC111009982 PE=3 SV=1)

HSP 1 Score: 761.1 bits (1964), Expect = 2.2e-216
Identity = 371/393 (94.40%), Postives = 383/393 (97.46%), Query Frame = 0

Query: 1   MGPIRGFKRKKKTEKKVDQNVLAASSLTSQPQPLDWWDEFSQRITGPLSQSKN-TKFESV 60
           MGPIRGFKRKKK EKKVDQNVLAA+SL+SQPQPLDWWD+FSQRITGPLSQSKN TKFESV
Sbjct: 1   MGPIRGFKRKKKAEKKVDQNVLAAASLSSQPQPLDWWDDFSQRITGPLSQSKNPTKFESV 60

Query: 61  FKISRKTFSYICSLVKEAMMAKTSNYTDLNGKPLSLNDQVAVALRRLSSGESLSNIGDLF 120
           FKISRKTFSYICSLVKEAMMAKTSN+TDLNGKPLS+NDQVAVALRRLSSGESLS IGD F
Sbjct: 61  FKISRKTFSYICSLVKEAMMAKTSNFTDLNGKPLSVNDQVAVALRRLSSGESLSIIGDSF 120

Query: 121 GMNQSSVSQITWRFVEAMEEKGLHHLSWPSTEEDMDQIKSKFKKIRGLPNCCGVIETTHI 180
           GMNQSSVSQITWRFVEAMEEKGLHHLSWPSTEEDMDQIKSKFKKI+GLPNCCGVIETTHI
Sbjct: 121 GMNQSSVSQITWRFVEAMEEKGLHHLSWPSTEEDMDQIKSKFKKIKGLPNCCGVIETTHI 180

Query: 181 MMTLPTAESANGVWLDREKNCSMILQVIVDPEMRFCDIITGWPGSLSDALVLQSSGFFKL 240
           MMTLPTAES NGVWLDREKNCSMILQVIVDPEMRFCDI+ GWPGSLSDALVLQSSGFFKL
Sbjct: 181 MMTLPTAESXNGVWLDREKNCSMILQVIVDPEMRFCDIMAGWPGSLSDALVLQSSGFFKL 240

Query: 241 SQDGERLSGKKMKLSESSEVGEYIIGDSGFPLLPWLLTPYQGKGLSDYQTEFNKRHFATR 300
           SQDGERL+GK MKLSESSE+GEYIIGDSGFPLLPWLLTPYQGKGLSDYQTEFNKRH+ATR
Sbjct: 241 SQDGERLNGKNMKLSESSELGEYIIGDSGFPLLPWLLTPYQGKGLSDYQTEFNKRHYATR 300

Query: 301 LVAQRALTRLKEMWKIIKGVMWKPDKHRLPRIILVCCLLHNIVIDMEDEVQDEMPLSHHH 360
           LVAQRALTRLKEMWKIIKGVMWKPDKHRLPRIILVCCLLHNIVIDMEDEVQDEMPLSHHH
Sbjct: 301 LVAQRALTRLKEMWKIIKGVMWKPDKHRLPRIILVCCLLHNIVIDMEDEVQDEMPLSHHH 360

Query: 361 DPSYRQQSCEFVDNTASIAREKLSMYLSGKLPP 393
           D  YRQQSC+FVDNTAS+ REKLSMYLSGKLPP
Sbjct: 361 DSGYRQQSCKFVDNTASVVREKLSMYLSGKLPP 393

BLAST of Lag0040755 vs. ExPASy TrEMBL
Match: A0A6J1J6K0 (protein ALP1-like OS=Cucurbita maxima OX=3661 GN=LOC111483923 PE=3 SV=1)

HSP 1 Score: 759.6 bits (1960), Expect = 6.4e-216
Identity = 371/392 (94.64%), Postives = 381/392 (97.19%), Query Frame = 0

Query: 1   MGPIRGFKRKKKTEKKVDQNVLAASSLTSQPQPLDWWDEFSQRITGPLSQSKNTKFESVF 60
           MGPIRGFKRKK   KKVDQNVL  SSLTSQPQPLDWWDEFSQRITGPLS+SKNT FESVF
Sbjct: 1   MGPIRGFKRKK---KKVDQNVLVPSSLTSQPQPLDWWDEFSQRITGPLSESKNTNFESVF 60

Query: 61  KISRKTFSYICSLVKEAMMAKTSNYTDLNGKPLSLNDQVAVALRRLSSGESLSNIGDLFG 120
           KISRKTFSYI SLVKEAMMAKTSN+TDLNGKPLS+NDQVAVALRRLSSGESLSNIGD FG
Sbjct: 61  KISRKTFSYISSLVKEAMMAKTSNFTDLNGKPLSINDQVAVALRRLSSGESLSNIGDSFG 120

Query: 121 MNQSSVSQITWRFVEAMEEKGLHHLSWPSTEEDMDQIKSKFKKIRGLPNCCGVIETTHIM 180
           MNQSSVSQITWRFVEAMEEKGLHHLSWPSTEE MDQIKSKFKKI+GLPNCCGVIETTHIM
Sbjct: 121 MNQSSVSQITWRFVEAMEEKGLHHLSWPSTEEGMDQIKSKFKKIKGLPNCCGVIETTHIM 180

Query: 181 MTLPTAESANGVWLDREKNCSMILQVIVDPEMRFCDIITGWPGSLSDALVLQSSGFFKLS 240
           MTLPT ESA+GVWLDREKNCSM+LQVIVDPEMRFCDIITGWPGSLSDALVLQSSGFF+LS
Sbjct: 181 MTLPTTESAHGVWLDREKNCSMLLQVIVDPEMRFCDIITGWPGSLSDALVLQSSGFFRLS 240

Query: 241 QDGERLSGKKMKLSESSEVGEYIIGDSGFPLLPWLLTPYQGKGLSDYQTEFNKRHFATRL 300
           QDGERL+GKKMKLSESSEVGEYIIGDSGFPLLPWLLTPYQGKGLSDYQTEFNKRHFATRL
Sbjct: 241 QDGERLNGKKMKLSESSEVGEYIIGDSGFPLLPWLLTPYQGKGLSDYQTEFNKRHFATRL 300

Query: 301 VAQRALTRLKEMWKIIKGVMWKPDKHRLPRIILVCCLLHNIVIDMEDEVQDEMPLSHHHD 360
           VAQRALTRLKEMWKIIKGVMWKPDKHRLPRI+LVCCLLHNIVIDMEDEVQDEMPLSHHHD
Sbjct: 301 VAQRALTRLKEMWKIIKGVMWKPDKHRLPRIVLVCCLLHNIVIDMEDEVQDEMPLSHHHD 360

Query: 361 PSYRQQSCEFVDNTASIAREKLSMYLSGKLPP 393
           PSYRQQSCEFVDNTAS+AREKLSMYL GKLPP
Sbjct: 361 PSYRQQSCEFVDNTASMAREKLSMYLLGKLPP 389

BLAST of Lag0040755 vs. TAIR 10
Match: AT3G55350.1 (PIF / Ping-Pong family of plant transposases )

HSP 1 Score: 510.0 bits (1312), Expect = 1.7e-144
Identity = 251/407 (61.67%), Postives = 306/407 (75.18%), Query Frame = 0

Query: 1   MGPIRGFKRKKKTEKKVDQNVLAASSLT------------------SQPQPLDWWDEFSQ 60
           MGPI+  K+KK+ EKKVD+NVL A++                    S  Q LDWWD FS+
Sbjct: 1   MGPIKTIKKKKRAEKKVDRNVLLAATAAATSASAAAALNNNDDDDDSSSQSLDWWDGFSR 60

Query: 61  RITGPLSQSKNTKFESVFKISRKTFSYICSLVKEAMMAKTSNYTDLNGKPLSLNDQVAVA 120
           RI G  +  K   FESVFKISRKTF YICSLVK    AK +N++D NG PLSLND+VAVA
Sbjct: 61  RIYGGSTDPKT--FESVFKISRKTFDYICSLVKADFTAKPANFSDSNGNPLSLNDRVAVA 120

Query: 121 LRRLSSGESLSNIGDLFGMNQSSVSQITWRFVEAMEEKGLHHLSWPSTEEDMDQIKSKFK 180
           LRRL SGESLS IG+ FGMNQS+VSQITWRFVE+MEE+ +HHLSWPS    +D+IKSKF+
Sbjct: 121 LRRLGSGESLSVIGETFGMNQSTVSQITWRFVESMEERAIHHLSWPS---KLDEIKSKFE 180

Query: 181 KIRGLPNCCGVIETTHIMMTLPTAESANGVWLDREKNCSMILQVIVDPEMRFCDIITGWP 240
           KI GLPNCCG I+ THI+M LP  E +N VWLD EKN SM LQ +VDP+MRF D+I GWP
Sbjct: 181 KISGLPNCCGAIDITHIVMNLPAVEPSNKVWLDGEKNFSMTLQAVVDPDMRFLDVIAGWP 240

Query: 241 GSLSDALVLQSSGFFKLSQDGERLSGKKMKLSESSEVGEYIIGDSGFPLLPWLLTPYQGK 300
           GSL+D +VL++SGF+KL + G+RL+G+K+ LSE +E+ EYI+GDSGFPLLPWLLTPYQGK
Sbjct: 241 GSLNDDVVLKNSGFYKLVEKGKRLNGEKLPLSERTELREYIVGDSGFPLLPWLLTPYQGK 300

Query: 301 GLSDYQTEFNKRHFATRLVAQRALTRLKEMWKIIKGVMWKPDKHRLPRIILVCCLLHNIV 360
             S  QTEFNKRH      AQ AL++LK+ W+II GVMW PD++RLPRII VCCLLHNI+
Sbjct: 301 PTSLPQTEFNKRHSEATKAAQMALSKLKDRWRIINGVMWMPDRNRLPRIIFVCCLLHNII 360

Query: 361 IDMEDEVQDEMPLSHHHDPSYRQQSCEFVDNTASIAREKLSMYLSGK 390
           IDMED+  D+ PLS  HD +YRQ+SC+  D  +S+ R++LS  L GK
Sbjct: 361 IDMEDQTLDDQPLSQQHDMNYRQRSCKLADEASSVLRDELSDQLCGK 402

BLAST of Lag0040755 vs. TAIR 10
Match: AT3G63270.1 (CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (InterPro:IPR006912); BEST Arabidopsis thaliana protein match is: PIF / Ping-Pong family of plant transposases (TAIR:AT3G55350.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 356.3 bits (913), Expect = 3.2e-98
Identity = 171/380 (45.00%), Postives = 256/380 (67.37%), Query Frame = 0

Query: 9   RKKKTEKKVDQNVLAASSLTSQPQPLDWWDEFSQRITGP-LSQSKNTKFESVFKISRKTF 68
           + KK  K  ++  + A  L  +    DWWD F  R + P +   ++  F+  F+ S+ TF
Sbjct: 17  KAKKLAKNKEKKRVNAVPLDPEAIDCDWWDTFWLRNSSPSVPSDEDYAFKHFFRASKTTF 76

Query: 69  SYICSLVKEAMMAK-TSNYTDLNGKPLSLNDQVAVALRRLSSGESLSNIGDLFGMNQSSV 128
           SYICSLV+E ++++  S   ++ G+ LS+  QVA+ALRRL+SG+S  ++G  FG+ QS+V
Sbjct: 77  SYICSLVREDLISRPPSGLINIEGRLLSVEKQVAIALRRLASGDSQVSVGAAFGVGQSTV 136

Query: 129 SQITWRFVEAMEEKGLHHLSWPSTEEDMDQIKSKFKKIRGLPNCCGVIETTHIMMTLPTA 188
           SQ+TWRF+EA+EE+  HHL WP ++  +++IKSKF+++ GLPNCCG I+TTHI+MTLP  
Sbjct: 137 SQVTWRFIEALEERAKHHLRWPDSDR-IEEIKSKFEEMYGLPNCCGAIDTTHIIMTLPAV 196

Query: 189 ESANGVWLDREKNCSMILQVIVDPEMRFCDIITGWPGSLSDALVLQSSGFFKLSQDGERL 248
           ++++  W D+EKN SM LQ + D EMRF +++TGWPG ++ + +L+ SGFFKL ++ + L
Sbjct: 197 QASDD-WCDQEKNYSMFLQGVFDHEMRFLNMVTGWPGGMTVSKLLKFSGFFKLCENAQIL 256

Query: 249 SGKKMKLSESSEVGEYIIGDSGFPLLPWLLTPYQGKGLSDYQTEFNKRHFATRLVAQRAL 308
            G    LS+ +++ EY++G   +PLLPWL+TP+     SD    FN+RH   R VA  A 
Sbjct: 257 DGNPKTLSQGAQIREYVVGGISYPLLPWLITPHDSDHPSDSMVAFNERHEKVRSVAATAF 316

Query: 309 TRLKEMWKIIKGVMWKPDKHRLPRIILVCCLLHNIVIDMEDEVQDEMPLSHHHDPSYRQQ 368
            +LK  W+I+  VMW+PD+ +LP IILVCCLLHNI+ID  D +Q+++PLS HHD  Y  +
Sbjct: 317 QQLKGSWRILSKVMWRPDRRKLPSIILVCCLLHNIIIDCGDYLQEDVPLSGHHDSGYADR 376

Query: 369 SCEFVDNTASIAREKLSMYL 387
            C+  +   S  R  L+ +L
Sbjct: 377 YCKQTEPLGSELRGCLTEHL 394

BLAST of Lag0040755 vs. TAIR 10
Match: AT5G12010.1 (unknown protein; INVOLVED IN: response to salt stress; LOCATED IN: chloroplast, plasma membrane, membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G29780.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 141.7 bits (356), Expect = 1.2e-33
Identity = 90/324 (27.78%), Postives = 164/324 (50.62%), Query Frame = 0

Query: 36  WWDEFSQRITGPLSQSKNTKFESVFKISRKTFSYICSLVKEAMMAKTSNYTDLNGKPLSL 95
           WW+E S R+  P        F+  F++S+ TF  IC  +  A+  + +   +     + +
Sbjct: 161 WWEECS-RLDYP-----EEDFKKAFRMSKSTFELICDELNSAVAKEDTALRN----AIPV 220

Query: 96  NDQVAVALRRLSSGESLSNIGDLFGMNQSSVSQITWRFVEAMEEKGL-HHLSWPSTEEDM 155
             +VAV + RL++GE L  +   FG+  S+  ++     +A+++  +  +L WP  +E +
Sbjct: 221 RQRVAVCIWRLATGEPLRLVSKKFGLGISTCHKLVLEVCKAIKDVLMPKYLQWPD-DESL 280

Query: 156 DQIKSKFKKIRGLPNCCGVIETTHIMMTLPTAESAN-----GVWLDREKNCSMILQVIVD 215
             I+ +F+ + G+PN  G + TTHI +  P    A+         +++ + S+ +Q +V+
Sbjct: 281 RNIRERFESVSGIPNVVGSMYTTHIPIIAPKISVASYFNKRHTERNQKTSYSITIQAVVN 340

Query: 216 PEMRFCDIITGWPGSLSDALVLQSSGFFKLSQDGERLSGKKMKLSESSEVGEYIIGDSGF 275
           P+  F D+  GWPGS+ D  VL+ S  ++ + +G  L G             ++ G  G 
Sbjct: 341 PKGVFTDLCIGWPGSMPDDKVLEKSLLYQRANNGGLLKGM------------WVAGGPGH 400

Query: 276 PLLPWLLTPYQGKGLSDYQTEFNKRHFATRLVAQRALTRLKEMWKIIKGVMWKPDKHRLP 335
           PLL W+L PY  + L+  Q  FN++    + VA+ A  RLK  W  ++    +     LP
Sbjct: 401 PLLDWVLVPYTQQNLTWTQHAFNEKMSEVQGVAKEAFGRLKGRWACLQ-KRTEVKLQDLP 460

Query: 336 RIILVCCLLHNIVIDMEDEVQDEM 354
            ++  CC+LHNI    E++++ E+
Sbjct: 461 TVLGACCVLHNICEMREEKMEPEL 460

BLAST of Lag0040755 vs. TAIR 10
Match: AT4G29780.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G12010.1); Has 945 Blast hits to 944 proteins in 87 species: Archae - 0; Bacteria - 0; Metazoa - 519; Fungi - 43; Plants - 365; Viruses - 0; Other Eukaryotes - 18 (source: NCBI BLink). )

HSP 1 Score: 125.2 bits (313), Expect = 1.2e-28
Identity = 97/363 (26.72%), Postives = 168/363 (46.28%), Query Frame = 0

Query: 35  DWWDEFSQRITGPLSQSKNTKFESVFKISRKTFSYICSLVKEAMMAKTSNYTDLNGKPLS 94
           DWWD  S+            +F   F++S+ TF+ IC  +   +  K +   D    P  
Sbjct: 198 DWWDRVSR------PDFPEDEFRREFRMSKSTFNLICEELDTTVTKKNTMLRDAIPAP-- 257

Query: 95  LNDQVAVALRRLSSGESLSNIGDLFGMNQSSVSQITWRFVEAMEEKGL-HHLSWPSTEED 154
              +V V + RL++G  L ++ + FG+  S+  ++      A+ +  +  +L WPS + +
Sbjct: 258 --KRVGVCVWRLATGAPLRHVSERFGLGISTCHKLVIEVCRAIYDVLMPKYLLWPS-DSE 317

Query: 155 MDQIKSKFKKIRGLPNCCGVIETTHIMMTLPTAESA-----NGVWLDREKNCSMILQVIV 214
           ++  K+KF+ +  +PN  G I TTHI +  P    A          +++ + S+ +Q +V
Sbjct: 318 INSTKAKFESVHKIPNVVGSIYTTHIPIIAPKVHVAAYFNKRHTERNQKTSYSITVQGVV 377

Query: 215 DPEMRFCDIITGWPGSLSDALVLQSSGFFKLSQDGERLSGKKMKLSESSEVGEYIIGDSG 274
           + +  F D+  G PGSL+D  +L+ S          R    +  L +S     +I+G+SG
Sbjct: 378 NADGIFTDVCIGNPGSLTDDQILEKSSL-------SRQRAARGMLRDS-----WIVGNSG 437

Query: 275 FPLLPWLLTPYQGKGLSDYQTEFNKRHFATRLVAQRALTRLKEMWKIIKGVMWKPDKHRL 334
           FPL  +LL PY  + L+  Q  FN+     + +A  A  RLK  W  ++    +     L
Sbjct: 438 FPLTDYLLVPYTRQNLTWTQHAFNESIGEIQGIATAAFERLKGRWACLQ-KRTEVKLQDL 497

Query: 335 PRIILVCCLLHNIVIDMEDEVQDEMPLSHHHD---PSYRQQSCEFVDNTASIAREKLSMY 389
           P ++  CC+LHNI    ++E+  E+      D   P    +S   V+    I+   L   
Sbjct: 498 PYVLGACCVLHNICEMRKEEMLPELKFEVFDDVAVPENNIRSASAVNTRDHISHNLLHRG 536

BLAST of Lag0040755 vs. TAIR 10
Match: AT1G72270.1 (CONTAINS InterPro DOMAIN/s: Ribosome 60S biogenesis N-terminal (InterPro:IPR021714); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G27010.1); Has 772 Blast hits to 657 proteins in 120 species: Archae - 0; Bacteria - 0; Metazoa - 344; Fungi - 94; Plants - 322; Viruses - 0; Other Eukaryotes - 12 (source: NCBI BLink). )

HSP 1 Score: 99.4 bits (246), Expect = 6.9e-21
Identity = 83/327 (25.38%), Postives = 140/327 (42.81%), Query Frame = 0

Query: 25  SSLTSQPQPLDWWDEFSQRITGPLSQSKNTKFESVFKISRKTFSYICSLVKEAMMAKTSN 84
           SS +S      W++ F   +T       + ++   F++S+ TF  + S++  + +     
Sbjct: 69  SSSSSAITTTTWFNRF---LTSATEDEDDPRWCLYFRMSKSTFFSLYSILSHSSL----- 128

Query: 85  YTDLNGKPLSLNDQVAVALRRLSSGESLSNIGDLFGMNQSS-VSQITWRFVEAMEEKGLH 144
                          A  + RL+ G S   +   FG + +S  S+  +   + + EK   
Sbjct: 129 ------------PSFAATIFRLAHGASYECLVHRFGFDSTSQASRSFFTVCKLINEK--- 188

Query: 145 HLSWPSTEEDMDQIKSKFKKIRGLPNCCGVIETTHIMMTLPTAESANGVWLDREKNCSMI 204
                   + +D  K  F     LPNC GV+                G  L  +   S++
Sbjct: 189 ------LSQQLDDPKPDFSP-NLLPNCYGVVGFGRF--------EVKGKLLGAKG--SIL 248

Query: 205 LQVIVDPEMRFCDIITGWPGSLSDALVLQSSGFFKLSQDGERLSGKKMKLSESSEVGEYI 264
           +Q +VD   RF DI  GWP ++    + + +  F +++  E LSG   KL     V  YI
Sbjct: 249 VQALVDSNGRFVDISAGWPSTMKPEAIFRQTKLFSIAE--EVLSGAPTKLGNGVLVPRYI 308

Query: 265 IGDSGFPLLPWLLTPYQ-GKGLSDYQTEFNKRHFATRLVAQRALTRLKEMWKIIKGVMWK 324
           +GDS  PLLPWL+TPY        ++ EFN          + A  +++  W+I+    WK
Sbjct: 309 LGDSCLPLLPWLVTPYDLTSDEESFREEFNNVVHTGLHSVEIAFAKVRARWRIL-DKKWK 352

Query: 325 PDK-HRLPRIILVCCLLHNIVIDMEDE 349
           P+    +P +I   CLLHN +++  D+
Sbjct: 369 PETIEFMPFVITTGCLLHNFLVNSGDD 352

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038891834.15.2e-22096.17protein ALP1-like [Benincasa hispida][more]
XP_004147700.16.4e-21894.64protein ALP1-like [Cucumis sativus] >KGN50531.1 hypothetical protein Csa_000507 ... [more]
XP_008461643.11.1e-21794.64PREDICTED: putative nuclease HARBI1 [Cucumis melo][more]
XP_022941714.12.7e-21694.90protein ALP1-like [Cucurbita moschata][more]
XP_022138922.14.6e-21694.40protein ALP1-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q9M2U32.4e-14361.67Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1[more]
Q94K494.4e-9745.00Protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1 OS=Arabidopsis thaliana OX=... [more]
Q6AZB82.8e-2726.99Putative nuclease HARBI1 OS=Danio rerio OX=7955 GN=harbi1 PE=2 SV=1[more]
Q8BR934.5e-2527.14Putative nuclease HARBI1 OS=Mus musculus OX=10090 GN=Harbi1 PE=2 SV=1[more]
Q96MB71.0e-2426.38Putative nuclease HARBI1 OS=Homo sapiens OX=9606 GN=HARBI1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KS643.1e-21894.64DDE Tnp4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G180900 PE... [more]
A0A1S3CEZ15.3e-21894.64putative nuclease HARBI1 OS=Cucumis melo OX=3656 GN=LOC103500196 PE=3 SV=1[more]
A0A6J1FP851.3e-21694.90protein ALP1-like OS=Cucurbita moschata OX=3662 GN=LOC111446995 PE=3 SV=1[more]
A0A6J1CCK22.2e-21694.40protein ALP1-like OS=Momordica charantia OX=3673 GN=LOC111009982 PE=3 SV=1[more]
A0A6J1J6K06.4e-21694.64protein ALP1-like OS=Cucurbita maxima OX=3661 GN=LOC111483923 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G55350.11.7e-14461.67PIF / Ping-Pong family of plant transposases [more]
AT3G63270.13.2e-9845.00CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (Int... [more]
AT5G12010.11.2e-3327.78unknown protein; INVOLVED IN: response to salt stress; LOCATED IN: chloroplast, ... [more]
AT4G29780.11.2e-2826.72unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT1G72270.16.9e-2125.38CONTAINS InterPro DOMAIN/s: Ribosome 60S biogenesis N-terminal (InterPro:IPR0217... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027806Harbinger transposase-derived nuclease domainPFAMPF13359DDE_Tnp_4coord: 175..340
e-value: 1.3E-29
score: 102.9
NoneNo IPR availablePANTHERPTHR22930UNCHARACTERIZEDcoord: 1..388
NoneNo IPR availablePANTHERPTHR22930:SF205PROTEIN ALP1-LIKEcoord: 1..388

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0040755.1Lag0040755.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding