Lag0040709 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0040709
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionJ domain-containing protein
Locationchr13: 7521400 .. 7523103 (-)
RNA-Seq ExpressionLag0040709
SyntenyLag0040709
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTGCCGCATGGCTTCCTCTGTACACACCCACTGCTGCAACGCCCAGACAGTATCCAACTCAAAGAAAGTTGGGATCATACAACACTTCTACTTCCAGAAAGCTTTATGGCAATACATTATCTTGCAGGGCAGCTTCTTCAATTACGGACTTTGATCTTTATGATCTTCTTGGCATCGACAACACCTCTGAACCATCACGGATTAAGGCAGCCTACCGTGCTCTCCAAAAGCAGTGCCACCCCGACATCGCTGGTCCTGCTGGCCATGACATGGCTATCATTCTCAATGAAGTGTATTCGGTTCTTTCGGATCCTAATTCTAGGTTGGCTTATGATAAGGTCAGAACTGAAACTCTATTCTAATTTGTGCATTTCATTTCAGAAACAAGATGGTTTTTGACAAAAATGATGTCCACAGGAGCAAGCAAAAATGGCAGAACTACGAGGTTATACAGGGAAGCCAGTTTATTCGGTGTGGTTAGGATCAGAAGGCGAACAACGGGCGGTTTTTGTAGATGAAGTGAAGTGCATTGGCTGCTTGAAATGTGCTTTATTTGCTGGGAAAACTTTTGCTGTCGAATCAGTGTATGGGAGAGCTAGAGTTGTGGCACAGTGGGCCGATCCTGAATATAAAGTGATGGAGGCCATTGAAGCTTGCCCAGTTGACTGCATTTCGTGAGTGTTTAATCAACCTGTTAAACTTCTTTCTAAACATTCAAATTTCCTTACTCTCTTGATTTGCAATATAAGTTCCTTTTTCTTGGCAAACTAAACCCAGGATGGTGGAAAGGTCAGATTTGGCAGCCCTAGAGTTTTTAATGTCAAAACAACCGCGTGGAAACGTAAGAGTTGGAATGGGCAACGCAGCTGGTGAACGAGTGTCAAACATTTTCACAGACGTGAAGAAGTTCCAAACCAGATTCAAGGAGGTTATGGAGAAAACTTCAAAACAGCATGTCAAGGTTAGACCCAAAGATACTAATGCTAATAGTTCGACATCACCATTAACTTAAAATGGCTCAAAATTGATTTAGTGGAAACGAGTGTCAAAACAGCATGTGATTGTTTTTTCAGGGGACAACCTTTGAGAGTGAAGCACAATTGTCTGCAATTCAAGCTATCAGATCCATATCAAATTGGCTATTTTGGCAGACAGCCACCCCAGTTGGACCTGGATCAAAACACAGCCAAAACCTGACACACATCCCCAGCAAATCCACACCAGAGATCAACAAACTTCAAGCTGCTGTTACTGCAAGGAAACAACTCAGGGAAAAAACTGAAGACAGAAATAGAACTGCAACAAAATACATAAAGAAGGATGAATACTGGGTTCCAACCACCCTTGCACTTCCAGCCTCGACTCAAACTTCGAACAGCATAATCTCAAGGCCAAGAGTGGAGACCAAACCTACCAAGGAATCAAAAGAACTTGGTTTTGAAGTTGGTGGTGCTGGTCATATTAGCCCCATGAGATTGGCATTACCAGTCTCTGTATCGATAATTGCAACCACTGTAATTCAGCAAATGGTGAGAGATGATGGTGCCAGCGGCTTGAAGGAACATATTGGAGGTTCTATGGCTCTGGAGTTGGTTAACAGTCAATGGATGCAGGTCATACAAACAGGAGTCACTTGGTATGTCATTGGAATGGCAGTGATGGGAATGGTAGAAATGATTGCAAGAAAGATTAGGCCTTAA

mRNA sequence

ATGACTGCCGCATGGCTTCCTCTGTACACACCCACTGCTGCAACGCCCAGACAGTATCCAACTCAAAGAAAGTTGGGATCATACAACACTTCTACTTCCAGAAAGCTTTATGGCAATACATTATCTTGCAGGGCAGCTTCTTCAATTACGGACTTTGATCTTTATGATCTTCTTGGCATCGACAACACCTCTGAACCATCACGGATTAAGGCAGCCTACCGTGCTCTCCAAAAGCAGTGCCACCCCGACATCGCTGGTCCTGCTGGCCATGACATGGCTATCATTCTCAATGAAGTGTATTCGGTTCTTTCGGATCCTAATTCTAGGTTGGCTTATGATAAGGAGCAAGCAAAAATGGCAGAACTACGAGGTTATACAGGGAAGCCAGTTTATTCGGTGTGGTTAGGATCAGAAGGCGAACAACGGGCGGTTTTTGTAGATGAAGTGAAGTGCATTGGCTGCTTGAAATGTGCTTTATTTGCTGGGAAAACTTTTGCTGTCGAATCAGTGTATGGGAGAGCTAGAGTTGTGGCACAGTGGGCCGATCCTGAATATAAAGTGATGGAGGCCATTGAAGCTTGCCCAGTTGACTGCATTTCGATGGTGGAAAGGTCAGATTTGGCAGCCCTAGAGTTTTTAATGTCAAAACAACCGCGTGGAAACGTAAGAGTTGGAATGGGCAACGCAGCTGGTGAACGAGTGTCAAACATTTTCACAGACGTGAAGAAGTTCCAAACCAGATTCAAGGAGGTTATGGAGAAAACTTCAAAACAGCATGTCAAGGGGACAACCTTTGAGAGTGAAGCACAATTGTCTGCAATTCAAGCTATCAGATCCATATCAAATTGGCTATTTTGGCAGACAGCCACCCCAGTTGGACCTGGATCAAAACACAGCCAAAACCTGACACACATCCCCAGCAAATCCACACCAGAGATCAACAAACTTCAAGCTGCTGTTACTGCAAGGAAACAACTCAGGGAAAAAACTGAAGACAGAAATAGAACTGCAACAAAATACATAAAGAAGGATGAATACTGGGTTCCAACCACCCTTGCACTTCCAGCCTCGACTCAAACTTCGAACAGCATAATCTCAAGGCCAAGAGTGGAGACCAAACCTACCAAGGAATCAAAAGAACTTGGTTTTGAAGTTGGTGGTGCTGGTCATATTAGCCCCATGAGATTGGCATTACCAGTCTCTGTATCGATAATTGCAACCACTGTAATTCAGCAAATGGTGAGAGATGATGGTGCCAGCGGCTTGAAGGAACATATTGGAGGTTCTATGGCTCTGGAGTTGGTTAACAGTCAATGGATGCAGGTCATACAAACAGGAGTCACTTGGTATGTCATTGGAATGGCAGTGATGGGAATGGTAGAAATGATTGCAAGAAAGATTAGGCCTTAA

Coding sequence (CDS)

ATGACTGCCGCATGGCTTCCTCTGTACACACCCACTGCTGCAACGCCCAGACAGTATCCAACTCAAAGAAAGTTGGGATCATACAACACTTCTACTTCCAGAAAGCTTTATGGCAATACATTATCTTGCAGGGCAGCTTCTTCAATTACGGACTTTGATCTTTATGATCTTCTTGGCATCGACAACACCTCTGAACCATCACGGATTAAGGCAGCCTACCGTGCTCTCCAAAAGCAGTGCCACCCCGACATCGCTGGTCCTGCTGGCCATGACATGGCTATCATTCTCAATGAAGTGTATTCGGTTCTTTCGGATCCTAATTCTAGGTTGGCTTATGATAAGGAGCAAGCAAAAATGGCAGAACTACGAGGTTATACAGGGAAGCCAGTTTATTCGGTGTGGTTAGGATCAGAAGGCGAACAACGGGCGGTTTTTGTAGATGAAGTGAAGTGCATTGGCTGCTTGAAATGTGCTTTATTTGCTGGGAAAACTTTTGCTGTCGAATCAGTGTATGGGAGAGCTAGAGTTGTGGCACAGTGGGCCGATCCTGAATATAAAGTGATGGAGGCCATTGAAGCTTGCCCAGTTGACTGCATTTCGATGGTGGAAAGGTCAGATTTGGCAGCCCTAGAGTTTTTAATGTCAAAACAACCGCGTGGAAACGTAAGAGTTGGAATGGGCAACGCAGCTGGTGAACGAGTGTCAAACATTTTCACAGACGTGAAGAAGTTCCAAACCAGATTCAAGGAGGTTATGGAGAAAACTTCAAAACAGCATGTCAAGGGGACAACCTTTGAGAGTGAAGCACAATTGTCTGCAATTCAAGCTATCAGATCCATATCAAATTGGCTATTTTGGCAGACAGCCACCCCAGTTGGACCTGGATCAAAACACAGCCAAAACCTGACACACATCCCCAGCAAATCCACACCAGAGATCAACAAACTTCAAGCTGCTGTTACTGCAAGGAAACAACTCAGGGAAAAAACTGAAGACAGAAATAGAACTGCAACAAAATACATAAAGAAGGATGAATACTGGGTTCCAACCACCCTTGCACTTCCAGCCTCGACTCAAACTTCGAACAGCATAATCTCAAGGCCAAGAGTGGAGACCAAACCTACCAAGGAATCAAAAGAACTTGGTTTTGAAGTTGGTGGTGCTGGTCATATTAGCCCCATGAGATTGGCATTACCAGTCTCTGTATCGATAATTGCAACCACTGTAATTCAGCAAATGGTGAGAGATGATGGTGCCAGCGGCTTGAAGGAACATATTGGAGGTTCTATGGCTCTGGAGTTGGTTAACAGTCAATGGATGCAGGTCATACAAACAGGAGTCACTTGGTATGTCATTGGAATGGCAGTGATGGGAATGGTAGAAATGATTGCAAGAAAGATTAGGCCTTAA

Protein sequence

MTAAWLPLYTPTAATPRQYPTQRKLGSYNTSTSRKLYGNTLSCRAASSITDFDLYDLLGIDNTSEPSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMAELRGYTGKPVYSVWLGSEGEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQWADPEYKVMEAIEACPVDCISMVERSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTDVKKFQTRFKEVMEKTSKQHVKGTTFESEAQLSAIQAIRSISNWLFWQTATPVGPGSKHSQNLTHIPSKSTPEINKLQAAVTARKQLREKTEDRNRTATKYIKKDEYWVPTTLALPASTQTSNSIISRPRVETKPTKESKELGFEVGGAGHISPMRLALPVSVSIIATTVIQQMVRDDGASGLKEHIGGSMALELVNSQWMQVIQTGVTWYVIGMAVMGMVEMIARKIRP
Homology
BLAST of Lag0040709 vs. NCBI nr
Match: KAG6600118.1 (Chaperone protein dnaJ C76, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 795.4 bits (2053), Expect = 2.6e-226
Identity = 410/468 (87.61%), Postives = 431/468 (92.09%), Query Frame = 0

Query: 1   MTAAWLPLYTPTAATPRQYPTQRKLGSYNTSTSRKLYGNTLSCRAASSITDFDLYDLLGI 60
           M AAWLPLYTP  AT  QYPTQRKLGSYN+STS+ LYGNTL+CRA SSITDFDLYDLLGI
Sbjct: 1   MNAAWLPLYTPIVATKIQYPTQRKLGSYNSSTSKMLYGNTLACRAGSSITDFDLYDLLGI 60

Query: 61  DNTSEPSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA 120
           DNTS+ SRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA
Sbjct: 61  DNTSDSSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA 120

Query: 121 ELRGYTGKPVYSVWLGSEGEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 180
           ELRGYTGK VYSVWLGSE EQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW
Sbjct: 121 ELRGYTGKSVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 180

Query: 181 ADPEYKVMEAIEACPVDCISMVERSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD 240
           ADPEYKVMEAIEACPVDCISMVERSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD
Sbjct: 181 ADPEYKVMEAIEACPVDCISMVERSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD 240

Query: 241 VKKFQTRFKEVMEKTSKQHVKGTTFESEAQLSAIQAIRSISNWLFWQTATPVGPGSKHSQ 300
           VKKFQTRFKE MEKTSKQHVKGTTFE++AQLSAIQAIRSISN LFWQTAT     SKHSQ
Sbjct: 241 VKKFQTRFKEAMEKTSKQHVKGTTFENKAQLSAIQAIRSISNCLFWQTATV----SKHSQ 300

Query: 301 NLTHIPSKSTPEINKLQAAVTARKQLREKTEDRNRTATKYIKKDEYWVPTTLALPASTQT 360
           NLT + +KSTP+INKLQAA+TARKQL+ KTED+N T TKYI KD+YWVPTTLALP STQT
Sbjct: 301 NLTLVVNKSTPQINKLQAAITARKQLKAKTEDKNGTTTKYI-KDDYWVPTTLALPVSTQT 360

Query: 361 SNSIISRPRVETKPTKESKELGFEVGGAGHISPMRLALPVSVSIIATTVIQQMVRDDGAS 420
             S IS PRVETK +KESK+L FEVGG  H SPMRLALPV +SI+ATTVIQQMV DDGAS
Sbjct: 361 PISTISNPRVETKRSKESKDLDFEVGGGDHTSPMRLALPVLISIVATTVIQQMVTDDGAS 420

Query: 421 GLKEHIGGSMALELVNSQWMQVIQTGVTWYVIGMAVMGMVEMIARKIR 469
           GLKEHIGGS+ALELVNS WMQ+IQ GVTWYVIG+AV+GMVEMIARKIR
Sbjct: 421 GLKEHIGGSVALELVNSHWMQLIQRGVTWYVIGIAVIGMVEMIARKIR 463

BLAST of Lag0040709 vs. NCBI nr
Match: XP_022942611.1 (chaperone protein dnaJ C76, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 791.6 bits (2043), Expect = 3.8e-225
Identity = 408/468 (87.18%), Postives = 428/468 (91.45%), Query Frame = 0

Query: 1   MTAAWLPLYTPTAATPRQYPTQRKLGSYNTSTSRKLYGNTLSCRAASSITDFDLYDLLGI 60
           M AAWLPLYTP  AT  QYPTQRKLGSY +STS+ LYGNTL+CRA SSITDFDLYDLLGI
Sbjct: 1   MNAAWLPLYTPIVATKIQYPTQRKLGSYYSSTSKMLYGNTLACRAGSSITDFDLYDLLGI 60

Query: 61  DNTSEPSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA 120
           DNTS+ SRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA
Sbjct: 61  DNTSDSSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA 120

Query: 121 ELRGYTGKPVYSVWLGSEGEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 180
           ELRGYTGK VYSVWLGSE EQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW
Sbjct: 121 ELRGYTGKSVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 180

Query: 181 ADPEYKVMEAIEACPVDCISMVERSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD 240
           ADPEYKVMEAIEACPVDCISMVERSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD
Sbjct: 181 ADPEYKVMEAIEACPVDCISMVERSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD 240

Query: 241 VKKFQTRFKEVMEKTSKQHVKGTTFESEAQLSAIQAIRSISNWLFWQTATPVGPGSKHSQ 300
           VKKFQTRFKE MEKTSKQHVKGTTFE++AQLSAIQAIRSISN LFWQTAT    GSKHSQ
Sbjct: 241 VKKFQTRFKEAMEKTSKQHVKGTTFENKAQLSAIQAIRSISNCLFWQTAT----GSKHSQ 300

Query: 301 NLTHIPSKSTPEINKLQAAVTARKQLREKTEDRNRTATKYIKKDEYWVPTTLALPASTQT 360
           NLT + +KSTP+INKLQAA+TARKQL+ KTEDRN T TKYI  D+YWVPTTLALP STQT
Sbjct: 301 NLTLVVNKSTPQINKLQAAITARKQLKAKTEDRNGTTTKYI-TDDYWVPTTLALPVSTQT 360

Query: 361 SNSIISRPRVETKPTKESKELGFEVGGAGHISPMRLALPVSVSIIATTVIQQMVRDDGAS 420
             S IS PRVETK +KESK+L FEVGG  H SPMRLALPV +SI+ATTVIQQMV DDGAS
Sbjct: 361 PISTISNPRVETKRSKESKDLDFEVGGGDHTSPMRLALPVLISIVATTVIQQMVTDDGAS 420

Query: 421 GLKEHIGGSMALELVNSQWMQVIQTGVTWYVIGMAVMGMVEMIARKIR 469
           GLKEHIGGS+ALE VNS WMQ+IQ GVTWYVIG+AV+GMVEMI RKIR
Sbjct: 421 GLKEHIGGSVALEFVNSHWMQLIQRGVTWYVIGIAVIGMVEMIGRKIR 463

BLAST of Lag0040709 vs. NCBI nr
Match: XP_038892355.1 (chaperone protein dnaJ C76, chloroplastic [Benincasa hispida])

HSP 1 Score: 788.9 bits (2036), Expect = 2.4e-224
Identity = 397/469 (84.65%), Postives = 424/469 (90.41%), Query Frame = 0

Query: 1   MTAAWLPLYTPTAATPRQYPTQRKLGSYNTSTSRKLYGNTLSCRAASSITDFDLYDLLGI 60
           M+A+WLPLYTPT    RQYP QR+L  Y  STSRKL+GNTLSCRAASSITDFDLYDLLGI
Sbjct: 44  MSASWLPLYTPT----RQYPIQRRLEPYIPSTSRKLHGNTLSCRAASSITDFDLYDLLGI 103

Query: 61  DNTSEPSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA 120
           DNT+ PSRIKAAYRALQK CHPDIAGPAGHDMAIILNE YSVLSDP SRLAYDKEQAKMA
Sbjct: 104 DNTANPSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPCSRLAYDKEQAKMA 163

Query: 121 ELRGYTGKPVYSVWLGSEGEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 180
           ELRGYTGKPVYSVWLGSE EQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW
Sbjct: 164 ELRGYTGKPVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 223

Query: 181 ADPEYKVMEAIEACPVDCISMVERSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD 240
           ADPEYKVMEAIEACPVDCISMVER+DLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD
Sbjct: 224 ADPEYKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD 283

Query: 241 VKKFQTRFKEVMEKTSKQHVKGTTFESEAQLSAIQAIRSISNWLFWQTATPVGPGSKHSQ 300
           VKKFQ RF E MEK +K+  KG TFESEAQL+AIQAIRSISNWLFWQTATP GPGSKH Q
Sbjct: 284 VKKFQIRFNEAMEKATKEQSKGATFESEAQLAAIQAIRSISNWLFWQTATPAGPGSKHGQ 343

Query: 301 NLTHIPSKSTPEINKLQAAVTARKQLREKTEDRNRTATKYIKKDEYWVPTTLALPASTQT 360
           NL    SK TPEIN+LQAAVTARKQ+REK EDRNRT  KY+ KD+YWVPTT ALPASTQ+
Sbjct: 344 NLARSASKFTPEINRLQAAVTARKQVREKAEDRNRTTAKYLYKDDYWVPTTFALPASTQS 403

Query: 361 SNSIISRPRVETKPTKESKELGFEVGGAGHISPMRLALPVSVSIIATTVIQQMVRDDGAS 420
            N+ IS+PRVETKPTKES ELGF+VG  GH+S MRLALPVS+SI+ATT+IQQMV +DGAS
Sbjct: 404 QNNTISKPRVETKPTKESGELGFDVGDGGHVSLMRLALPVSISIVATTIIQQMVENDGAS 463

Query: 421 GLKEHIGGSMALELVNSQWMQVIQTGVTWYVIGMAVMGMVEMIARKIRP 470
           GLKEH  GSMAL+LVNS+WMQV+ TGVTWY+IGMAV+GMV MIARK RP
Sbjct: 464 GLKEHAAGSMALDLVNSRWMQVMLTGVTWYIIGMAVLGMVGMIARKFRP 508

BLAST of Lag0040709 vs. NCBI nr
Match: XP_023532693.1 (chaperone protein dnaJ C76, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 788.9 bits (2036), Expect = 2.4e-224
Identity = 407/468 (86.97%), Postives = 429/468 (91.67%), Query Frame = 0

Query: 1   MTAAWLPLYTPTAATPRQYPTQRKLGSYNTSTSRKLYGNTLSCRAASSITDFDLYDLLGI 60
           MTAAWLPLYTP  AT  Q PT+RKLGSYN STS+ LYGNTL+CRA SSITDFDLYDLLGI
Sbjct: 38  MTAAWLPLYTPVVATKIQNPTRRKLGSYNFSTSKMLYGNTLACRAGSSITDFDLYDLLGI 97

Query: 61  DNTSEPSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA 120
           DNTS+ SRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA
Sbjct: 98  DNTSDSSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA 157

Query: 121 ELRGYTGKPVYSVWLGSEGEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 180
           ELRGYTGK VYSVWLGSE E+RAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW
Sbjct: 158 ELRGYTGKSVYSVWLGSESERRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 217

Query: 181 ADPEYKVMEAIEACPVDCISMVERSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD 240
           ADPEYKVMEAIEACPVDCISMVERSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD
Sbjct: 218 ADPEYKVMEAIEACPVDCISMVERSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD 277

Query: 241 VKKFQTRFKEVMEKTSKQHVKGTTFESEAQLSAIQAIRSISNWLFWQTATPVGPGSKHSQ 300
           VKKFQTRFKE MEKTSKQHVKGTTFE++AQLSAIQAIRSISN LFWQTAT    GSKHSQ
Sbjct: 278 VKKFQTRFKEAMEKTSKQHVKGTTFENKAQLSAIQAIRSISNCLFWQTAT----GSKHSQ 337

Query: 301 NLTHIPSKSTPEINKLQAAVTARKQLREKTEDRNRTATKYIKKDEYWVPTTLALPASTQT 360
           NLT + + STP+INKLQAA+TARKQL+ KTED+N T TKYI KD+YWVPTTLALP STQT
Sbjct: 338 NLTLVVNTSTPQINKLQAAITARKQLKAKTEDKNGTTTKYI-KDDYWVPTTLALPVSTQT 397

Query: 361 SNSIISRPRVETKPTKESKELGFEVGGAGHISPMRLALPVSVSIIATTVIQQMVRDDGAS 420
             S IS PRVETK +KESK+L FEVGG  H SPMRLALPV +SI+ATTVIQQMV DDGAS
Sbjct: 398 PISTISNPRVETKRSKESKDLDFEVGGGDHTSPMRLALPVLISIVATTVIQQMVTDDGAS 457

Query: 421 GLKEHIGGSMALELVNSQWMQVIQTGVTWYVIGMAVMGMVEMIARKIR 469
           GLKEHIGGS+ALELVNS WMQ+IQ GVTWYVIG+AV+GMVE IARKIR
Sbjct: 458 GLKEHIGGSVALELVNSHWMQLIQRGVTWYVIGIAVIGMVEKIARKIR 500

BLAST of Lag0040709 vs. NCBI nr
Match: XP_011654903.1 (chaperone protein dnaJ C76, chloroplastic [Cucumis sativus] >KGN50447.1 hypothetical protein Csa_000192 [Cucumis sativus])

HSP 1 Score: 786.2 bits (2029), Expect = 1.6e-223
Identity = 396/469 (84.43%), Postives = 422/469 (89.98%), Query Frame = 0

Query: 1   MTAAWLPLYTPTAATPRQYPTQRKLGSYNTSTSRKLYGNTLSCRAASSITDFDLYDLLGI 60
           M+A+WLPLYTPTA    QYP QRKLGSYN STSRKL+GNTLSC+AASSITDFDLYDLLGI
Sbjct: 1   MSASWLPLYTPTA----QYPIQRKLGSYNPSTSRKLHGNTLSCKAASSITDFDLYDLLGI 60

Query: 61  DNTSEPSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA 120
           DNTS PSRIKAAYRALQK CHPDIAGPAGHDMAIILNE YSVLSDP+SRLAYDKEQAKMA
Sbjct: 61  DNTSHPSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPSSRLAYDKEQAKMA 120

Query: 121 ELRGYTGKPVYSVWLGSEGEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 180
           ELRGYTGKPVYSVWLGSE EQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW
Sbjct: 121 ELRGYTGKPVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 180

Query: 181 ADPEYKVMEAIEACPVDCISMVERSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD 240
           ADPEYKVMEAIEACPVDCISMVER+DLAALEFLMSKQPRGNVRVGMGN AGERVSNIFTD
Sbjct: 181 ADPEYKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRVGMGNTAGERVSNIFTD 240

Query: 241 VKKFQTRFKEVMEKTSKQHVKGTTFESEAQLSAIQAIRSISNWLFWQTATPVGPGSKHSQ 300
           VKKFQ +F E MEK  K+  KG TFESE QL+AIQAIRSISNWLFWQTATPVGPGSK SQ
Sbjct: 241 VKKFQIKFNEAMEKAMKEQSKGATFESEGQLAAIQAIRSISNWLFWQTATPVGPGSKQSQ 300

Query: 301 NLTHIPSKSTPEINKLQAAVTARKQLREKTEDRNRTATKYIKKDEYWVPTTLALPASTQT 360
           +L    SK TPEINKLQAA TARKQ+REK EDRNRT TKY+ +D+YWVPTT ALPASTQ+
Sbjct: 301 SLARSASKFTPEINKLQAAATARKQIREKAEDRNRTTTKYLYRDDYWVPTTFALPASTQS 360

Query: 361 SNSIISRPRVETKPTKESKELGFEVGGAGHISPMRLALPVSVSIIATTVIQQMVRDDGAS 420
            N+ IS+P VETKPTK+S+ LG +V   GH+SPMRL LPVS+SIIAT +IQQMVR+DGAS
Sbjct: 361 PNNPISKPSVETKPTKQSRGLGSDVSRGGHVSPMRLVLPVSISIIATAIIQQMVRNDGAS 420

Query: 421 GLKEHIGGSMALELVNSQWMQVIQTGVTWYVIGMAVMGMVEMIARKIRP 470
            LKEH  GSMALELVNS WMQVI TGVTWY+IGMAVMGM+EMIARK RP
Sbjct: 421 ELKEHAAGSMALELVNSHWMQVILTGVTWYIIGMAVMGMLEMIARKFRP 465

BLAST of Lag0040709 vs. ExPASy Swiss-Prot
Match: Q9FMX6 (Chaperone protein dnaJ C76, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DJC76 PE=2 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 5.8e-128
Identity = 253/471 (53.72%), Postives = 317/471 (67.30%), Query Frame = 0

Query: 10  TPTAATPRQYPTQRKLGSYNTSTSRKLY----GNTLSCRA---ASSITDFDLYDLLGIDN 69
           TP   +P   P      ++  STS+KL          CRA   +SSITDFDLYDLLGID 
Sbjct: 2   TPAIFSPTTLPP--STATWPCSTSQKLITVRSPLKFKCRATSSSSSITDFDLYDLLGIDR 61

Query: 70  TSEPSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMAEL 129
           +S+ S+IK+AYRALQK+CHPDIAG  GHDMAIILNE Y +LSDP SR AYDKEQAK+ EL
Sbjct: 62  SSDKSQIKSAYRALQKRCHPDIAGDPGHDMAIILNEAYQLLSDPISRQAYDKEQAKLEEL 121

Query: 130 RGYTGKPVYSVWLGSEGEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQWAD 189
           RGYTGKP+YSVW G E EQRA FVDEVKC+GCLKCAL A KTFA+E+ YGRARVVAQWAD
Sbjct: 122 RGYTGKPIYSVWCGPETEQRAAFVDEVKCVGCLKCALCAEKTFAIETAYGRARVVAQWAD 181

Query: 190 PEYKVMEAIEACPVDCISMVERSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTDVK 249
           PE K+ EAIEACPVDCISMVERSDLA LEFLMSKQPRGNVR+G+GN  GERVSN+F DVK
Sbjct: 182 PESKIKEAIEACPVDCISMVERSDLAPLEFLMSKQPRGNVRIGVGNTVGERVSNVFVDVK 241

Query: 250 KFQTRFKEVMEKTSKQHVKGTTFESEAQLSAIQAIRSISNWLFWQTA---TPVGPGSKHS 309
           KFQ R+ + M +T+K+     T + E Q+SA++AIRSISNWL+W+++    P+ P S  S
Sbjct: 242 KFQERYAKAMSRTTKE-----TSQREVQISAVEAIRSISNWLYWRSSPYTKPLSPESNMS 301

Query: 310 QNLTHIPSKSTPEINKLQAAVTARKQLREKTEDRNRTATKYIKKDEYWVPTTLALPASTQ 369
              T       P+I KLQ  V A KQ  +    + +  + Y+  ++YW P+  ALP+S  
Sbjct: 302 LTFTKRKKAVDPDIRKLQDVVAAMKQADQSGRTKEK-GSAYLLGEDYWSPSNAALPSSGN 361

Query: 370 TSNS-IISRPRV--ETKPTKESKELGFEVGGAGHISPMRLALPVSVSIIATTVIQQMVRD 429
            + S   S P+V  +T P++E      E      I       P+  +I+A  ++Q     
Sbjct: 362 NNGSKASSNPQVTRKTFPSEEKPTSRRENRRQFRIK----KFPIGTAIVAVFLVQYQASY 421

Query: 430 DGASGLKEHIGGSMALELVNSQWMQVIQTGVTWYVIGMAVMGMVEMIARKI 468
             AS L +HIGGS+AL +VNS W Q++  GVTWY IG  ++ +VE +  K+
Sbjct: 422 RAASELNDHIGGSLALSIVNSPWQQILLAGVTWYFIGAMLLQLVEAVQHKL 460

BLAST of Lag0040709 vs. ExPASy Swiss-Prot
Match: Q8G6C6 (Chaperone protein DnaJ OS=Bifidobacterium longum (strain NCC 2705) OX=206672 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 7.9e-08
Identity = 27/61 (44.26%), Postives = 36/61 (59.02%), Query Frame = 0

Query: 53  DLYDLLGIDNTSEPSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAY 112
           D Y+ LG++  +    IK AYR L ++ HPDIAGP   D    +N  Y VLS+P+ R  Y
Sbjct: 3   DYYETLGVERGASDDEIKKAYRKLSRKYHPDIAGPEFEDKFKEVNNAYDVLSNPDKRRMY 62

Query: 113 D 114
           D
Sbjct: 63  D 63

BLAST of Lag0040709 vs. ExPASy Swiss-Prot
Match: B5YAR4 (Chaperone protein DnaJ OS=Dictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12) OX=309799 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.0e-07
Identity = 28/66 (42.42%), Postives = 38/66 (57.58%), Query Frame = 0

Query: 50  TDFDLYDLLGIDNTSEPSRIKAAYRALQKQCHPDI-AGPAGHDMAIILNEVYSVLSDPNS 109
           T  D Y++LG+   +    IK AYR L +Q HPD+   P+ H+    +NE Y VLSDP  
Sbjct: 3   TKKDYYEILGVPRNATQDEIKQAYRRLVRQYHPDLNKDPSAHEKFKEINEAYEVLSDPQK 62

Query: 110 RLAYDK 115
           R  YD+
Sbjct: 63  RAQYDQ 68

BLAST of Lag0040709 vs. ExPASy Swiss-Prot
Match: Q6A997 (Chaperone protein DnaJ 1 OS=Cutibacterium acnes (strain DSM 16379 / KPA171202) OX=267747 GN=dnaJ1 PE=3 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 3.0e-07
Identity = 24/73 (32.88%), Postives = 38/73 (52.05%), Query Frame = 0

Query: 53  DLYDLLGIDNTSEPSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAY 112
           D Y++LG+ + +    IK AYR    + HPD+AGP   +    + E Y +L DP  R  +
Sbjct: 4   DYYEILGVSHDASADEIKKAYRRKAMKLHPDVAGPGSEEEFKKVQEAYEILQDPQKRAVF 63

Query: 113 DKEQAKMAELRGY 126
           D+     A + G+
Sbjct: 64  DRGGDPNARMGGF 76

BLAST of Lag0040709 vs. ExPASy Swiss-Prot
Match: Q92BN9 (Chaperone protein DnaJ OS=Listeria innocua serovar 6a (strain ATCC BAA-680 / CLIP 11262) OX=272626 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 3.0e-07
Identity = 29/63 (46.03%), Postives = 36/63 (57.14%), Query Frame = 0

Query: 53  DLYDLLGIDNTSEPSRIKAAYRALQKQCHPDIAGPAGHDMAI-ILNEVYSVLSDPNSRLA 112
           D Y++LGI  ++    IK AYR L KQ HPDI   AG D     ++E Y  LSDP  R  
Sbjct: 5   DYYEVLGISKSASADEIKKAYRKLSKQYHPDINKEAGADEKFKEISEAYEALSDPQKRAQ 64

Query: 113 YDK 115
           YD+
Sbjct: 65  YDQ 67

BLAST of Lag0040709 vs. ExPASy TrEMBL
Match: A0A6J1FPC3 (chaperone protein dnaJ C76, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111447595 PE=4 SV=1)

HSP 1 Score: 791.6 bits (2043), Expect = 1.8e-225
Identity = 408/468 (87.18%), Postives = 428/468 (91.45%), Query Frame = 0

Query: 1   MTAAWLPLYTPTAATPRQYPTQRKLGSYNTSTSRKLYGNTLSCRAASSITDFDLYDLLGI 60
           M AAWLPLYTP  AT  QYPTQRKLGSY +STS+ LYGNTL+CRA SSITDFDLYDLLGI
Sbjct: 1   MNAAWLPLYTPIVATKIQYPTQRKLGSYYSSTSKMLYGNTLACRAGSSITDFDLYDLLGI 60

Query: 61  DNTSEPSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA 120
           DNTS+ SRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA
Sbjct: 61  DNTSDSSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA 120

Query: 121 ELRGYTGKPVYSVWLGSEGEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 180
           ELRGYTGK VYSVWLGSE EQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW
Sbjct: 121 ELRGYTGKSVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 180

Query: 181 ADPEYKVMEAIEACPVDCISMVERSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD 240
           ADPEYKVMEAIEACPVDCISMVERSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD
Sbjct: 181 ADPEYKVMEAIEACPVDCISMVERSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD 240

Query: 241 VKKFQTRFKEVMEKTSKQHVKGTTFESEAQLSAIQAIRSISNWLFWQTATPVGPGSKHSQ 300
           VKKFQTRFKE MEKTSKQHVKGTTFE++AQLSAIQAIRSISN LFWQTAT    GSKHSQ
Sbjct: 241 VKKFQTRFKEAMEKTSKQHVKGTTFENKAQLSAIQAIRSISNCLFWQTAT----GSKHSQ 300

Query: 301 NLTHIPSKSTPEINKLQAAVTARKQLREKTEDRNRTATKYIKKDEYWVPTTLALPASTQT 360
           NLT + +KSTP+INKLQAA+TARKQL+ KTEDRN T TKYI  D+YWVPTTLALP STQT
Sbjct: 301 NLTLVVNKSTPQINKLQAAITARKQLKAKTEDRNGTTTKYI-TDDYWVPTTLALPVSTQT 360

Query: 361 SNSIISRPRVETKPTKESKELGFEVGGAGHISPMRLALPVSVSIIATTVIQQMVRDDGAS 420
             S IS PRVETK +KESK+L FEVGG  H SPMRLALPV +SI+ATTVIQQMV DDGAS
Sbjct: 361 PISTISNPRVETKRSKESKDLDFEVGGGDHTSPMRLALPVLISIVATTVIQQMVTDDGAS 420

Query: 421 GLKEHIGGSMALELVNSQWMQVIQTGVTWYVIGMAVMGMVEMIARKIR 469
           GLKEHIGGS+ALE VNS WMQ+IQ GVTWYVIG+AV+GMVEMI RKIR
Sbjct: 421 GLKEHIGGSVALEFVNSHWMQLIQRGVTWYVIGIAVIGMVEMIGRKIR 463

BLAST of Lag0040709 vs. ExPASy TrEMBL
Match: A0A0A0KPY4 (J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G175710 PE=4 SV=1)

HSP 1 Score: 786.2 bits (2029), Expect = 7.7e-224
Identity = 396/469 (84.43%), Postives = 422/469 (89.98%), Query Frame = 0

Query: 1   MTAAWLPLYTPTAATPRQYPTQRKLGSYNTSTSRKLYGNTLSCRAASSITDFDLYDLLGI 60
           M+A+WLPLYTPTA    QYP QRKLGSYN STSRKL+GNTLSC+AASSITDFDLYDLLGI
Sbjct: 1   MSASWLPLYTPTA----QYPIQRKLGSYNPSTSRKLHGNTLSCKAASSITDFDLYDLLGI 60

Query: 61  DNTSEPSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA 120
           DNTS PSRIKAAYRALQK CHPDIAGPAGHDMAIILNE YSVLSDP+SRLAYDKEQAKMA
Sbjct: 61  DNTSHPSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPSSRLAYDKEQAKMA 120

Query: 121 ELRGYTGKPVYSVWLGSEGEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 180
           ELRGYTGKPVYSVWLGSE EQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW
Sbjct: 121 ELRGYTGKPVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 180

Query: 181 ADPEYKVMEAIEACPVDCISMVERSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD 240
           ADPEYKVMEAIEACPVDCISMVER+DLAALEFLMSKQPRGNVRVGMGN AGERVSNIFTD
Sbjct: 181 ADPEYKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRVGMGNTAGERVSNIFTD 240

Query: 241 VKKFQTRFKEVMEKTSKQHVKGTTFESEAQLSAIQAIRSISNWLFWQTATPVGPGSKHSQ 300
           VKKFQ +F E MEK  K+  KG TFESE QL+AIQAIRSISNWLFWQTATPVGPGSK SQ
Sbjct: 241 VKKFQIKFNEAMEKAMKEQSKGATFESEGQLAAIQAIRSISNWLFWQTATPVGPGSKQSQ 300

Query: 301 NLTHIPSKSTPEINKLQAAVTARKQLREKTEDRNRTATKYIKKDEYWVPTTLALPASTQT 360
           +L    SK TPEINKLQAA TARKQ+REK EDRNRT TKY+ +D+YWVPTT ALPASTQ+
Sbjct: 301 SLARSASKFTPEINKLQAAATARKQIREKAEDRNRTTTKYLYRDDYWVPTTFALPASTQS 360

Query: 361 SNSIISRPRVETKPTKESKELGFEVGGAGHISPMRLALPVSVSIIATTVIQQMVRDDGAS 420
            N+ IS+P VETKPTK+S+ LG +V   GH+SPMRL LPVS+SIIAT +IQQMVR+DGAS
Sbjct: 361 PNNPISKPSVETKPTKQSRGLGSDVSRGGHVSPMRLVLPVSISIIATAIIQQMVRNDGAS 420

Query: 421 GLKEHIGGSMALELVNSQWMQVIQTGVTWYVIGMAVMGMVEMIARKIRP 470
            LKEH  GSMALELVNS WMQVI TGVTWY+IGMAVMGM+EMIARK RP
Sbjct: 421 ELKEHAAGSMALELVNSHWMQVILTGVTWYIIGMAVMGMLEMIARKFRP 465

BLAST of Lag0040709 vs. ExPASy TrEMBL
Match: A0A6J1JBT2 (chaperone protein dnaJ C76, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111485354 PE=4 SV=1)

HSP 1 Score: 779.2 bits (2011), Expect = 9.4e-222
Identity = 402/468 (85.90%), Postives = 424/468 (90.60%), Query Frame = 0

Query: 1   MTAAWLPLYTPTAATPRQYPTQRKLGSYNTSTSRKLYGNTLSCRAASSITDFDLYDLLGI 60
           MTAAWLPLYTP  AT  Q PT+RKLGSYN S S+ LYGNTL+CRA SSITDFDLYDLLGI
Sbjct: 1   MTAAWLPLYTPIVATKIQNPTRRKLGSYNFSFSKMLYGNTLACRAGSSITDFDLYDLLGI 60

Query: 61  DNTSEPSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA 120
           DNTS+ SRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA
Sbjct: 61  DNTSDSSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA 120

Query: 121 ELRGYTGKPVYSVWLGSEGEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 180
           ELRGYTGK VYSVWLGSE EQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW
Sbjct: 121 ELRGYTGKSVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 180

Query: 181 ADPEYKVMEAIEACPVDCISMVERSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD 240
           ADPEYKVMEAIEACPVDCISMVERSDLAALEFLMSKQPRGNVRVGMGNAA ERVSNIFTD
Sbjct: 181 ADPEYKVMEAIEACPVDCISMVERSDLAALEFLMSKQPRGNVRVGMGNAASERVSNIFTD 240

Query: 241 VKKFQTRFKEVMEKTSKQHVKGTTFESEAQLSAIQAIRSISNWLFWQTATPVGPGSKHSQ 300
           VKKFQ RFKE MEKTS QHVKGTTFE++AQLSAIQAIRSISN LFWQTAT    G+KHSQ
Sbjct: 241 VKKFQIRFKEAMEKTSNQHVKGTTFENKAQLSAIQAIRSISNCLFWQTAT----GTKHSQ 300

Query: 301 NLTHIPSKSTPEINKLQAAVTARKQLREKTEDRNRTATKYIKKDEYWVPTTLALPASTQT 360
           NL  + +KSTP+INKLQAA+TARKQL+ KTED+N T TKYI KD+YWVPTTLALP STQT
Sbjct: 301 NLMLVVNKSTPQINKLQAAITARKQLKAKTEDKNETTTKYI-KDDYWVPTTLALPVSTQT 360

Query: 361 SNSIISRPRVETKPTKESKELGFEVGGAGHISPMRLALPVSVSIIATTVIQQMVRDDGAS 420
             S IS PRVETK +KESK+L FEVGG  H SPMRLALPV +SI+AT VIQQMV DDGAS
Sbjct: 361 PISTISNPRVETKRSKESKDLDFEVGGGDHTSPMRLALPVLISIVATNVIQQMVTDDGAS 420

Query: 421 GLKEHIGGSMALELVNSQWMQVIQTGVTWYVIGMAVMGMVEMIARKIR 469
           G KEHIGGS+ALELVNS WMQ+IQ GVTWYVIG+AV+GMVEMIARKIR
Sbjct: 421 GFKEHIGGSVALELVNSHWMQLIQRGVTWYVIGIAVIGMVEMIARKIR 463

BLAST of Lag0040709 vs. ExPASy TrEMBL
Match: A0A5A7SY19 (Putative DNAJ heat shock N-terminal domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00570 PE=4 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 2.1e-221
Identity = 392/469 (83.58%), Postives = 419/469 (89.34%), Query Frame = 0

Query: 1   MTAAWLPLYTPTAATPRQYPTQRKLGSYNTSTSRKLYGNTLSCRAASSITDFDLYDLLGI 60
           M+A+WLPLYTPT    +QYP QRKLGSYN STSRKL+ NTLSC+AASSITDFDLYDLLGI
Sbjct: 1   MSASWLPLYTPT----KQYPIQRKLGSYNPSTSRKLHANTLSCKAASSITDFDLYDLLGI 60

Query: 61  DNTSEPSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA 120
           DNTS PSRIKAAYRALQK CHPDIAGPAGHDMAIILNE YSVLSDP+SRLAYDKEQAKMA
Sbjct: 61  DNTSNPSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPSSRLAYDKEQAKMA 120

Query: 121 ELRGYTGKPVYSVWLGSEGEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 180
           ELRGYTGKPVYSVWLGSE EQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW
Sbjct: 121 ELRGYTGKPVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 180

Query: 181 ADPEYKVMEAIEACPVDCISMVERSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD 240
           ADPEYKVMEAIEACPVDCISMVER+DLAALEFLMSKQPRGNVRVGMGN AGERVSNIFTD
Sbjct: 181 ADPEYKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRVGMGNTAGERVSNIFTD 240

Query: 241 VKKFQTRFKEVMEKTSKQHVKGTTFESEAQLSAIQAIRSISNWLFWQTATPVGPGSKHSQ 300
           VKKFQ RF E MEK +K+  KG TF+SE QL+AIQAIRSISNWLFWQTATPVGPGSK SQ
Sbjct: 241 VKKFQIRFNEAMEKATKEQSKGATFDSEGQLAAIQAIRSISNWLFWQTATPVGPGSKQSQ 300

Query: 301 NLTHIPSKSTPEINKLQAAVTARKQLREKTEDRNRTATKYIKKDEYWVPTTLALPASTQT 360
           +LT   SK TPEINKLQAAVTARKQ+REK E RNR  TKY+ +D+YWVPTT ALPASTQ 
Sbjct: 301 SLTRSASKFTPEINKLQAAVTARKQIREKAEGRNRITTKYLSRDDYWVPTTFALPASTQC 360

Query: 361 SNSIISRPRVETKPTKESKELGFEVGGAGHISPMRLALPVSVSIIATTVIQQMVRDDGAS 420
            N+ IS+P VETKPTK+S++LG +V    H+SPMRL LPVS+SIIAT +IQQMVR+DGA 
Sbjct: 361 PNNPISKPSVETKPTKQSRDLGSDVSSGVHVSPMRLILPVSISIIATAIIQQMVRNDGAD 420

Query: 421 GLKEHIGGSMALELVNSQWMQVIQTGVTWYVIGMAVMGMVEMIARKIRP 470
            LKEH  GSMALELVNS WMQVI TGVTWY+IGMAV GMVEMIARK RP
Sbjct: 421 ELKEHAAGSMALELVNSHWMQVILTGVTWYIIGMAVTGMVEMIARKFRP 465

BLAST of Lag0040709 vs. ExPASy TrEMBL
Match: A0A6J1GZ80 (chaperone protein dnaJ C76, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111458571 PE=4 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 2.1e-221
Identity = 394/469 (84.01%), Postives = 421/469 (89.77%), Query Frame = 0

Query: 1   MTAAWLPLYTPT-AATPRQYPTQRKLGSYNTSTSRKLYGNTLSCRAASSITDFDLYDLLG 60
           M+ +WLPLYT T AAT RQYPTQ KLGSYN+STSRK YGNTLSCRAASSITDFDLYDLLG
Sbjct: 1   MSTSWLPLYTHTAAATARQYPTQTKLGSYNSSTSRKPYGNTLSCRAASSITDFDLYDLLG 60

Query: 61  IDNTSEPSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKM 120
           ID++S+ SRIKAAYRALQKQCHPDIAGPAGHDM+IILNE YS+LSDP+SRLAYDKEQAKM
Sbjct: 61  IDSSSDSSRIKAAYRALQKQCHPDIAGPAGHDMSIILNEAYSLLSDPSSRLAYDKEQAKM 120

Query: 121 AELRGYTGKPVYSVWLGSEGEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQ 180
           AELRGYTG PVYSVWLGSE EQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQ
Sbjct: 121 AELRGYTGNPVYSVWLGSENEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQ 180

Query: 181 WADPEYKVMEAIEACPVDCISMVERSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFT 240
           WADPEYKVMEAI+ CPVDCISMVERSDLAALEFLMSKQPRGNVRVGM NAAGERVSNIFT
Sbjct: 181 WADPEYKVMEAIQTCPVDCISMVERSDLAALEFLMSKQPRGNVRVGMSNAAGERVSNIFT 240

Query: 241 DVKKFQTRFKEVMEKTSKQHVKGTTFESEAQLSAIQAIRSISNWLFWQTATPVGPGSKHS 300
           DVKKFQ RFKE MEK SK+H KG TFE+EAQL+AIQ IRSISNWLFWQTATP GPGSK  
Sbjct: 241 DVKKFQARFKEAMEKASKEHSKGATFENEAQLAAIQTIRSISNWLFWQTATPAGPGSKQG 300

Query: 301 QNLTHIPSKSTPEINKLQAAVTARKQLREKTEDRNRTATKYIKKDEYWVPTTLALPASTQ 360
           QNLTHI +KSTPEIN+LQAAVTARKQ+R K EDRNR   K+I KD+YWVPTTLALP STQ
Sbjct: 301 QNLTHIATKSTPEINRLQAAVTARKQIRAKAEDRNRATEKHIYKDDYWVPTTLALPTSTQ 360

Query: 361 TSNSIISRPRVETKPTKESKELGFEVGGAGHISPMRLALPVSVSIIATTVIQQMVRDDGA 420
           T NS IS+ RVETKP KE + L    G  GH+SPMRLALP+S+SIIAT  IQQM+R+DGA
Sbjct: 361 TPNSTISKSRVETKPIKEPRGL----GDGGHVSPMRLALPISISIIATAAIQQMMRNDGA 420

Query: 421 SGLKEHIGGSMALELVNSQWMQVIQTGVTWYVIGMAVMGMVEMIARKIR 469
           S LKEHIGGSMALELVNS WMQV+ TGVTWY+IGM VMGMVEMI RKIR
Sbjct: 421 SSLKEHIGGSMALELVNSHWMQVMLTGVTWYIIGMVVMGMVEMIGRKIR 465

BLAST of Lag0040709 vs. TAIR 10
Match: AT5G23240.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 459.1 bits (1180), Expect = 4.1e-129
Identity = 253/471 (53.72%), Postives = 317/471 (67.30%), Query Frame = 0

Query: 10  TPTAATPRQYPTQRKLGSYNTSTSRKLY----GNTLSCRA---ASSITDFDLYDLLGIDN 69
           TP   +P   P      ++  STS+KL          CRA   +SSITDFDLYDLLGID 
Sbjct: 2   TPAIFSPTTLPP--STATWPCSTSQKLITVRSPLKFKCRATSSSSSITDFDLYDLLGIDR 61

Query: 70  TSEPSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMAEL 129
           +S+ S+IK+AYRALQK+CHPDIAG  GHDMAIILNE Y +LSDP SR AYDKEQAK+ EL
Sbjct: 62  SSDKSQIKSAYRALQKRCHPDIAGDPGHDMAIILNEAYQLLSDPISRQAYDKEQAKLEEL 121

Query: 130 RGYTGKPVYSVWLGSEGEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQWAD 189
           RGYTGKP+YSVW G E EQRA FVDEVKC+GCLKCAL A KTFA+E+ YGRARVVAQWAD
Sbjct: 122 RGYTGKPIYSVWCGPETEQRAAFVDEVKCVGCLKCALCAEKTFAIETAYGRARVVAQWAD 181

Query: 190 PEYKVMEAIEACPVDCISMVERSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTDVK 249
           PE K+ EAIEACPVDCISMVERSDLA LEFLMSKQPRGNVR+G+GN  GERVSN+F DVK
Sbjct: 182 PESKIKEAIEACPVDCISMVERSDLAPLEFLMSKQPRGNVRIGVGNTVGERVSNVFVDVK 241

Query: 250 KFQTRFKEVMEKTSKQHVKGTTFESEAQLSAIQAIRSISNWLFWQTA---TPVGPGSKHS 309
           KFQ R+ + M +T+K+     T + E Q+SA++AIRSISNWL+W+++    P+ P S  S
Sbjct: 242 KFQERYAKAMSRTTKE-----TSQREVQISAVEAIRSISNWLYWRSSPYTKPLSPESNMS 301

Query: 310 QNLTHIPSKSTPEINKLQAAVTARKQLREKTEDRNRTATKYIKKDEYWVPTTLALPASTQ 369
              T       P+I KLQ  V A KQ  +    + +  + Y+  ++YW P+  ALP+S  
Sbjct: 302 LTFTKRKKAVDPDIRKLQDVVAAMKQADQSGRTKEK-GSAYLLGEDYWSPSNAALPSSGN 361

Query: 370 TSNS-IISRPRV--ETKPTKESKELGFEVGGAGHISPMRLALPVSVSIIATTVIQQMVRD 429
            + S   S P+V  +T P++E      E      I       P+  +I+A  ++Q     
Sbjct: 362 NNGSKASSNPQVTRKTFPSEEKPTSRRENRRQFRIK----KFPIGTAIVAVFLVQYQASY 421

Query: 430 DGASGLKEHIGGSMALELVNSQWMQVIQTGVTWYVIGMAVMGMVEMIARKI 468
             AS L +HIGGS+AL +VNS W Q++  GVTWY IG  ++ +VE +  K+
Sbjct: 422 RAASELNDHIGGSLALSIVNSPWQQILLAGVTWYFIGAMLLQLVEAVQHKL 460

BLAST of Lag0040709 vs. TAIR 10
Match: AT2G42750.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 118.6 bits (296), Expect = 1.3e-26
Identity = 69/183 (37.70%), Postives = 97/183 (53.01%), Query Frame = 0

Query: 53  DLYDLLGIDNTSEPSRIKAAYRALQKQCHPDIAG--PAGHDMAIILNEVYSVLSDPNSRL 112
           D Y +LG+   +    IK AY    K CHPD++G  P   +  + +N++Y +LSDP  R+
Sbjct: 76  DYYAVLGLLPDATQEEIKKAYYNCMKSCHPDLSGNDPETTNFCMFINDIYEILSDPVQRM 135

Query: 113 AYDKEQAKMAELRGYTGKPVYSVWLGSEGEQRAVFVDEVKCIGCLKCALFAGKTFAVESV 172
            YD       E+ GYT   + + +L     +  VFVDE  CIGC  CA  A   F +E  
Sbjct: 136 VYD-------EIHGYTVTAI-NPFLDDSTPRDHVFVDEFACIGCKNCANVAPDIFQIEED 195

Query: 173 YGRARVVAQWADPEYKVMEAIEACPVDCISMVERSDLAALEFLMSKQPRGNVRV---GMG 231
           +GRAR   Q  +P+  V +A+E CPVDCI     + L+ LE  M +  R NV +   GMG
Sbjct: 196 FGRARACNQRGNPDL-VQQAVETCPVDCIHQTSAAQLSLLEDEMRRVERVNVALMLSGMG 249

BLAST of Lag0040709 vs. TAIR 10
Match: AT3G05345.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 65.5 bits (158), Expect = 1.3e-10
Identity = 52/189 (27.51%), Postives = 84/189 (44.44%), Query Frame = 0

Query: 31  STSRKLYGNTLSCRAASSITDFDLYDLLGIDNTSEPSRIKAAYRALQKQCHPDIAGPAGH 90
           STS++   +         ++    Y +LG++ +   S +KAA+RA  KQ HPD+     +
Sbjct: 22  STSQRFIPSCRGKNREDPLSSSSPYSILGVEPSCSSSELKAAFRAKVKQYHPDVNKDGSN 81

Query: 91  DMAII--LNEVYSVLSDPNSRLAYDKEQAKMAELRGYTGKPVYSVWLGSEGEQRAVFVDE 150
              +I  + + Y +L++      Y + +    E       P        E E   VFV+E
Sbjct: 82  SDIMIRRIIQAYEMLTN------YSRSEIIEGECLDPFDHP--------ECEALDVFVNE 141

Query: 151 VKCIG---CLKCALFAGKTFAVESVYGRARVVAQWADPEYKVMEAIEACPVDCISMVERS 210
           V C+G      C   A   F+ +S  G AR ++Q    +Y+V  A+  CP +CI  V  S
Sbjct: 142 VLCVGKRCSYPCFETASHVFSCDS-SGTARAMSQGHGEDYRVQSAVNQCPRNCIHYVTPS 195

Query: 211 DLAALEFLM 215
               LE L+
Sbjct: 202 QRIILEELL 195

BLAST of Lag0040709 vs. TAIR 10
Match: AT4G39150.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 52.0 bits (123), Expect = 1.5e-06
Identity = 34/109 (31.19%), Postives = 43/109 (39.45%), Query Frame = 0

Query: 49  ITDFDLYDLLGIDNTSEPSRIKAAYRALQKQCHPD--IAGPAGHDMAIILNEVYSVLSDP 108
           + + + YD+LG+   +  + IK AY    +Q HPD     P       IL E Y VL DP
Sbjct: 2   VKESEYYDILGVKIDASGAEIKKAYYVQARQVHPDKNPGDPQAAKNFQILGEAYQVLGDP 61

Query: 109 NSRLAYDKEQAKMAELRGYTGKPVYSVWLGSEGEQRAVFVDEVKCIGCL 156
             R AYDK                     G EG Q+   VD     G L
Sbjct: 62  EKRTAYDK--------------------YGKEGVQQDAMVDPAAVFGML 90

BLAST of Lag0040709 vs. TAIR 10
Match: AT4G39150.2 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 52.0 bits (123), Expect = 1.5e-06
Identity = 34/109 (31.19%), Postives = 43/109 (39.45%), Query Frame = 0

Query: 49  ITDFDLYDLLGIDNTSEPSRIKAAYRALQKQCHPD--IAGPAGHDMAIILNEVYSVLSDP 108
           + + + YD+LG+   +  + IK AY    +Q HPD     P       IL E Y VL DP
Sbjct: 2   VKESEYYDILGVKIDASGAEIKKAYYVQARQVHPDKNPGDPQAAKNFQILGEAYQVLGDP 61

Query: 109 NSRLAYDKEQAKMAELRGYTGKPVYSVWLGSEGEQRAVFVDEVKCIGCL 156
             R AYDK                     G EG Q+   VD     G L
Sbjct: 62  EKRTAYDK--------------------YGKEGVQQDAMVDPAAVFGML 90

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6600118.12.6e-22687.61Chaperone protein dnaJ C76, chloroplastic, partial [Cucurbita argyrosperma subsp... [more]
XP_022942611.13.8e-22587.18chaperone protein dnaJ C76, chloroplastic-like [Cucurbita moschata][more]
XP_038892355.12.4e-22484.65chaperone protein dnaJ C76, chloroplastic [Benincasa hispida][more]
XP_023532693.12.4e-22486.97chaperone protein dnaJ C76, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_011654903.11.6e-22384.43chaperone protein dnaJ C76, chloroplastic [Cucumis sativus] >KGN50447.1 hypothet... [more]
Match NameE-valueIdentityDescription
Q9FMX65.8e-12853.72Chaperone protein dnaJ C76, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DJC... [more]
Q8G6C67.9e-0844.26Chaperone protein DnaJ OS=Bifidobacterium longum (strain NCC 2705) OX=206672 GN=... [more]
B5YAR41.0e-0742.42Chaperone protein DnaJ OS=Dictyoglomus thermophilum (strain ATCC 35947 / DSM 396... [more]
Q6A9973.0e-0732.88Chaperone protein DnaJ 1 OS=Cutibacterium acnes (strain DSM 16379 / KPA171202) O... [more]
Q92BN93.0e-0746.03Chaperone protein DnaJ OS=Listeria innocua serovar 6a (strain ATCC BAA-680 / CLI... [more]
Match NameE-valueIdentityDescription
A0A6J1FPC31.8e-22587.18chaperone protein dnaJ C76, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A0A0KPY47.7e-22484.43J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G175710 PE=4 SV=1[more]
A0A6J1JBT29.4e-22285.90chaperone protein dnaJ C76, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A5A7SY192.1e-22183.58Putative DNAJ heat shock N-terminal domain-containing protein OS=Cucumis melo va... [more]
A0A6J1GZ802.1e-22184.01chaperone protein dnaJ C76, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=... [more]
Match NameE-valueIdentityDescription
AT5G23240.14.1e-12953.72DNAJ heat shock N-terminal domain-containing protein [more]
AT2G42750.11.3e-2637.70DNAJ heat shock N-terminal domain-containing protein [more]
AT3G05345.11.3e-1027.51Chaperone DnaJ-domain superfamily protein [more]
AT4G39150.11.5e-0631.19DNAJ heat shock N-terminal domain-containing protein [more]
AT4G39150.21.5e-0631.19DNAJ heat shock N-terminal domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001623DnaJ domainSMARTSM00271dnaj_3coord: 52..108
e-value: 6.4E-13
score: 58.9
IPR001623DnaJ domainPFAMPF00226DnaJcoord: 53..113
e-value: 4.7E-13
score: 49.1
IPR001623DnaJ domainPROSITEPS50076DNAJ_2coord: 53..116
score: 14.28688
IPR001623DnaJ domainCDDcd06257DnaJcoord: 53..105
e-value: 2.34809E-13
score: 62.5629
IPR036869Chaperone J-domain superfamilyGENE3D1.10.287.110DnaJ domaincoord: 40..127
e-value: 3.0E-15
score: 58.1
IPR036869Chaperone J-domain superfamilySUPERFAMILY46565Chaperone J-domaincoord: 50..120
NoneNo IPR availablePFAMPF13370Fer4_13coord: 146..200
e-value: 2.6E-11
score: 43.9
NoneNo IPR availableGENE3D3.30.70.20coord: 141..203
e-value: 3.0E-15
score: 58.2
NoneNo IPR availablePANTHERPTHR45295CHAPERONE PROTEIN DNAJ C76, CHLOROPLASTICcoord: 32..466
NoneNo IPR availablePANTHERPTHR45295:SF1CHAPERONE PROTEIN DNAJ C76, CHLOROPLASTICcoord: 32..466
NoneNo IPR availableSUPERFAMILY548624Fe-4S ferredoxinscoord: 143..203

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0040709.1Lag0040709.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane