Lag0040650 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0040650
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Description3-ketoacyl-CoA synthase
Locationchr13: 6941775 .. 6942259 (-)
RNA-Seq ExpressionLag0040650
SyntenyLag0040650
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTCGATTTTGGCCAGGGGAATTCACCTGACCTTCACGTGCCACCAACGGTAAATTTGTATCACTAAATTAGTTGAAAACTCATGCATCGATAGACTGATATGATGTTAAACTCTAACACTGATTTAAAAGTTTAAGTAGCAATTAATTAATTAATTAATTTTGTTTATACTTTTTAACAAAGGATGAAGACGATGGCGGGTATCGTGGGTTTAGGCTAACGAAGAACCTTCCAAAAGCAGCAGCGAAAATATTTGCAATGAATTTAAGAGTTTTGGTGCCAAAGATTTTGCCATTAAGGGAGCTGCTTCGTTACGCCATAGCTAATTCTCTCACCCAAAACAAACCAAAGGAAGCCATGGGAGGGACTTCTCTAGGTCAAGCTCCAATTATAGGTTTGAATCTCAAGACCGGTGTCGATCACTTTTGCATTCACCCCGGTGGGAGGGCCGTTATCGACAGTCTTAGAACAACCATATTGTAA

mRNA sequence

ATGGTCGATTTTGGCCAGGGGAATTCACCTGACCTTCACGTGCCACCAACGGATGAAGACGATGGCGGGTATCGTGGGTTTAGGCTAACGAAGAACCTTCCAAAAGCAGCAGCGAAAATATTTGCAATGAATTTAAGAGTTTTGGTGCCAAAGATTTTGCCATTAAGGGAGCTGCTTCGTTACGCCATAGCTAATTCTCTCACCCAAAACAAACCAAAGGAAGCCATGGGAGGGACTTCTCTAGGTCAAGCTCCAATTATAGGTTTGAATCTCAAGACCGGTGTCGATCACTTTTGCATTCACCCCGGTGGGAGGGCCGTTATCGACAGTCTTAGAACAACCATATTGTAA

Coding sequence (CDS)

ATGGTCGATTTTGGCCAGGGGAATTCACCTGACCTTCACGTGCCACCAACGGATGAAGACGATGGCGGGTATCGTGGGTTTAGGCTAACGAAGAACCTTCCAAAAGCAGCAGCGAAAATATTTGCAATGAATTTAAGAGTTTTGGTGCCAAAGATTTTGCCATTAAGGGAGCTGCTTCGTTACGCCATAGCTAATTCTCTCACCCAAAACAAACCAAAGGAAGCCATGGGAGGGACTTCTCTAGGTCAAGCTCCAATTATAGGTTTGAATCTCAAGACCGGTGTCGATCACTTTTGCATTCACCCCGGTGGGAGGGCCGTTATCGACAGTCTTAGAACAACCATATTGTAA

Protein sequence

MVDFGQGNSPDLHVPPTDEDDGGYRGFRLTKNLPKAAAKIFAMNLRVLVPKILPLRELLRYAIANSLTQNKPKEAMGGTSLGQAPIIGLNLKTGVDHFCIHPGGRAVIDSLRTTIL
Homology
[Close]
BLAST of Lag0040650 vs. NCBI nr
Match: XP_038893179.1 (LOW QUALITY PROTEIN: 3-ketoacyl-CoA synthase 19-like [Benincasa hispida])

HSP 1 Score: 161.8 bits (408), Expect = 3.6e-36
Identity = 81/95 (85.26%), Postives = 86/95 (90.53%), Query Frame = 0 

Query: 19  EDDGGYRGFRLTKNLPKAAAKIFAMNLRVLVPKILPLRELLRYAIANSLTQ--NKPKEAM 78
           EDD GYRGFRLTKNLPKAAAKIFAMNLRVLVPKILPLRELLRY IANS++Q     KEAM
Sbjct: 265 EDDDGYRGFRLTKNLPKAAAKIFAMNLRVLVPKILPLRELLRYVIANSVSQKGKLAKEAM 324

Query: 79  GGTSLGQAPIIGLNLKTGVDHFCIHPGGRAVIDSL 112
           GG ++GQ PIIGLNLKTGVDHFCIHPGGRAVIDS+
Sbjct: 325 GGVAVGQGPIIGLNLKTGVDHFCIHPGGRAVIDSV 359

[Close]
BLAST of Lag0040650 vs. NCBI nr
Match: XP_023548644.1 (3-ketoacyl-CoA synthase 19-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 159.5 bits (402), Expect = 1.8e-35
Identity = 78/93 (83.87%), Postives = 82/93 (88.17%), Query Frame = 0 

Query: 19  EDDGGYRGFRLTKNLPKAAAKIFAMNLRVLVPKILPLRELLRYAIANSLTQNKPKEAMGG 78
           EDD GYRGFRLTKNLPKAAAKIFAMNLRVLVPKILPLRELL   IANSL+Q KPKEAMGG
Sbjct: 262 EDDSGYRGFRLTKNLPKAAAKIFAMNLRVLVPKILPLRELLHLVIANSLSQKKPKEAMGG 321

Query: 79  TSLGQAPIIGLNLKTGVDHFCIHPGGRAVIDSL 112
              G   ++GLNLKTGVDHFCIHPGGRAVIDS+
Sbjct: 322 GGGGGGQVVGLNLKTGVDHFCIHPGGRAVIDSV 354

[Close]
BLAST of Lag0040650 vs. NCBI nr
Match: KAG6600081.1 (3-ketoacyl-CoA synthase 19, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 157.9 bits (398), Expect = 5.2e-35
Identity = 79/93 (84.95%), Postives = 83/93 (89.25%), Query Frame = 0 

Query: 19  EDDGGYRGFRLTKNLPKAAAKIFAMNLRVLVPKILPLRELLRYAIANSLTQNKPKEAMGG 78
           EDD GYRGFRLTKNLPKAAAKIFAMNLRVLVPKILPLRELL  AIANSL+Q KPKEAMGG
Sbjct: 262 EDDNGYRGFRLTKNLPKAAAKIFAMNLRVLVPKILPLRELLHLAIANSLSQKKPKEAMGG 321

Query: 79  TSLGQAPIIGLNLKTGVDHFCIHPGGRAVIDSL 112
              G   ++GLNLKTGVDHFCIHPGGRAVIDS+
Sbjct: 322 GG-GGGQVVGLNLKTGVDHFCIHPGGRAVIDSV 353

[Close]
BLAST of Lag0040650 vs. NCBI nr
Match: KAG7030749.1 (3-ketoacyl-CoA synthase 19, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 157.9 bits (398), Expect = 5.2e-35
Identity = 79/93 (84.95%), Postives = 83/93 (89.25%), Query Frame = 0 

Query: 19  EDDGGYRGFRLTKNLPKAAAKIFAMNLRVLVPKILPLRELLRYAIANSLTQNKPKEAMGG 78
           EDD GYRGFRLTKNLPKAAAKIFAMNLRVLVPKILPLRELL  AIANSL+Q KPKEAMGG
Sbjct: 290 EDDNGYRGFRLTKNLPKAAAKIFAMNLRVLVPKILPLRELLHLAIANSLSQKKPKEAMGG 349

Query: 79  TSLGQAPIIGLNLKTGVDHFCIHPGGRAVIDSL 112
              G   ++GLNLKTGVDHFCIHPGGRAVIDS+
Sbjct: 350 ---GGGQVVGLNLKTGVDHFCIHPGGRAVIDSV 379

[Close]
BLAST of Lag0040650 vs. NCBI nr
Match: XP_022941767.1 (3-ketoacyl-CoA synthase 19-like [Cucurbita moschata])

HSP 1 Score: 157.9 bits (398), Expect = 5.2e-35
Identity = 79/93 (84.95%), Postives = 83/93 (89.25%), Query Frame = 0 

Query: 19  EDDGGYRGFRLTKNLPKAAAKIFAMNLRVLVPKILPLRELLRYAIANSLTQNKPKEAMGG 78
           EDD GYRGFRLTKNLPKAAAKIFAMNLRVLVPKILPLRELL  AIANSL+Q KPKEAMGG
Sbjct: 262 EDDNGYRGFRLTKNLPKAAAKIFAMNLRVLVPKILPLRELLHLAIANSLSQKKPKEAMGG 321

Query: 79  TSLGQAPIIGLNLKTGVDHFCIHPGGRAVIDSL 112
              G   ++GLNLKTGVDHFCIHPGGRAVIDS+
Sbjct: 322 ---GGGQVVGLNLKTGVDHFCIHPGGRAVIDSV 351

[Close]
BLAST of Lag0040650 vs. ExPASy Swiss-Prot
Match: Q9LZ72 (3-ketoacyl-CoA synthase 19 OS=Arabidopsis thaliana OX=3702 GN=KCS19 PE=2 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 7.1e-19
Identity = 50/96 (52.08%), Postives = 65/96 (67.71%), Query Frame = 0 

Query: 19  EDDGGYRGFRLTKNLPKAAAKIFAMNLRVLVPKILPLRELLRYAIANSL---TQNKPKEA 78
           ED  G+ GF LTK L KAAA+    NL+VL+P++LP++EL+RYAI  +L   T  K + A
Sbjct: 266 EDRDGHPGFLLTKYLKKAAARALTKNLQVLLPRVLPVKELIRYAIVRALKRRTSAKREPA 325

Query: 79  MGGTSLGQAPIIGLNLKTGVDHFCIHPGGRAVIDSL 112
             G        IGLNLKTG+ HFCIHPGGRA+I+ +
Sbjct: 326 SSG--------IGLNLKTGLQHFCIHPGGRAIIEGV 353

[Close]
BLAST of Lag0040650 vs. ExPASy Swiss-Prot
Match: Q9LQP8 (3-ketoacyl-CoA synthase 3 OS=Arabidopsis thaliana OX=3702 GN=KCS3 PE=2 SV=3)

HSP 1 Score: 91.3 bits (225), Expect = 7.9e-18
Identity = 46/98 (46.94%), Postives = 64/98 (65.31%), Query Frame = 0 

Query: 19  EDDGGYRGFRLTKNLPKAAAKIFAMNLRVLVPKILPLRELLRYAIANSLTQNKPKEAMGG 78
           ED+ G+ G  L K LPKAA + F  NL+V+ PKILP+ ELLR+ +   L + +   + G 
Sbjct: 262 EDELGHIGVHLDKTLPKAATRAFIDNLKVITPKILPVTELLRFMLCLLLKKLRSSPSKGS 321

Query: 79  TSLGQAP-----IIGLNLKTGVDHFCIHPGGRAVIDSL 112
           T++ QA        G+N KTG+DHFCIH GG+AVID++
Sbjct: 322 TNVTQAAPKAGVKAGINFKTGIDHFCIHTGGKAVIDAI 359

[Close]
BLAST of Lag0040650 vs. ExPASy Swiss-Prot
Match: Q9SIB2 (3-ketoacyl-CoA synthase 12 OS=Arabidopsis thaliana OX=3702 GN=KCS12 PE=2 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 3.9e-17
Identity = 46/97 (47.42%), Postives = 65/97 (67.01%), Query Frame = 0 

Query: 19  EDDGGYRGFRLTKNLPKAAAKIFAMNLRVLVPKILPLRELLRYAIANSLTQNKPKE--AM 78
           ED+ G  GF L KNLPKAA + F  NL+V+ PKILP+ EL+R+ +   + + K ++  + 
Sbjct: 262 EDEQGRVGFYLGKNLPKAATRAFVENLKVITPKILPVTELIRFMLKLLIKKIKIRQNPSK 321

Query: 79  GGTSL--GQAPIIGLNLKTGVDHFCIHPGGRAVIDSL 112
           G T+L  G     G+N KTG++HFCIH GG+AVID +
Sbjct: 322 GSTNLPPGTPLKAGINFKTGIEHFCIHTGGKAVIDGI 358

[Close]
BLAST of Lag0040650 vs. ExPASy Swiss-Prot
Match: Q4V3C9 (3-ketoacyl-CoA synthase 8 OS=Arabidopsis thaliana OX=3702 GN=KCS8 PE=2 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 3.9e-09
Identity = 36/98 (36.73%), Postives = 49/98 (50.00%), Query Frame = 0 

Query: 18  DEDDGGYRGFRLTKNLPKAAAKIFAMNLRVLVPKILPLRELLRYAIANSLTQNKPKEAMG 77
           +ED+ G  G  LTKNLP  AA+   +N+  L P +LPL+E L + I     +    E   
Sbjct: 306 EEDEDGIIGVTLTKNLPMVAARTLKINIATLGPLVLPLKEKLAFFITFVKKKYFKPELRN 365

Query: 78  GTSLGQAPIIGLNLKTGVDHFCIHPGGRAVIDSLRTTI 116
            T          + K   +HFCIH GGRA+ID L   +
Sbjct: 366 YTP---------DFKLAFEHFCIHAGGRALIDELEKNL 394

[Close]
BLAST of Lag0040650 vs. ExPASy Swiss-Prot
Match: Q9SYZ0 (3-ketoacyl-CoA synthase 16 OS=Arabidopsis thaliana OX=3702 GN=KCS16 PE=2 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 6.3e-07
Identity = 31/98 (31.63%), Postives = 49/98 (50.00%), Query Frame = 0 

Query: 18  DEDDGGYRGFRLTKNLPKAAAKIFAMNLRVLVPKILPLRELLRYAIANSLTQNKPKEAMG 77
           +ED+ G  G  L+KNLP  AA+   +N+  L P +LP+ E   + +     +   K+ + 
Sbjct: 314 EEDEDGIVGVSLSKNLPMVAARTLKINIATLGPLVLPISEKFHFFV-----RFVKKKFLN 373

Query: 78  GTSLGQAPIIGLNLKTGVDHFCIHPGGRAVIDSLRTTI 116
                  P    + K   +HFCIH GGRA+ID +   +
Sbjct: 374 PKLKHYIP----DFKLAFEHFCIHAGGRALIDEMEKNL 402

The following BLAST results are available for this feature:
BLAST of Lag0040650 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038893179.13.6e-3685.26LOW QUALITY PROTEIN: 3-ketoacyl-CoA synthase 19-like [Benincasa hispida][more]
XP_023548644.11.8e-3583.873-ketoacyl-CoA synthase 19-like [Cucurbita pepo subsp. pepo][more]
KAG6600081.15.2e-3584.953-ketoacyl-CoA synthase 19, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7030749.15.2e-3584.953-ketoacyl-CoA synthase 19, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022941767.15.2e-3584.953-ketoacyl-CoA synthase 19-like [Cucurbita moschata][more]
BLAST of Lag0040650 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9LZ727.1e-1952.083-ketoacyl-CoA synthase 19 OS=Arabidopsis thaliana OX=3702 GN=KCS19 PE=2 SV=1[more]
Q9LQP87.9e-1846.943-ketoacyl-CoA synthase 3 OS=Arabidopsis thaliana OX=3702 GN=KCS3 PE=2 SV=3[more]
Q9SIB23.9e-1747.423-ketoacyl-CoA synthase 12 OS=Arabidopsis thaliana OX=3702 GN=KCS12 PE=2 SV=1[more]
Q4V3C93.9e-0936.733-ketoacyl-CoA synthase 8 OS=Arabidopsis thaliana OX=3702 GN=KCS8 PE=2 SV=1[more]
Q9SYZ06.3e-0731.633-ketoacyl-CoA synthase 16 OS=Arabidopsis thaliana OX=3702 GN=KCS16 PE=2 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013601FAE1/Type III polyketide synthase-like proteinPFAMPF08392FAE1_CUT1_RppAcoord: 18..64
e-value: 7.6E-10
score: 38.6
IPR016039Thiolase-likeGENE3D3.40.47.10coord: 2..115
e-value: 2.0E-6
score: 28.9
IPR016039Thiolase-likeSUPERFAMILY53901Thiolase-likecoord: 16..112
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..24
NoneNo IPR availablePANTHERPTHR31561:SF1063-KETOACYL-COA SYNTHASE 19coord: 18..111
IPR012392Very-long-chain 3-ketoacyl-CoA synthasePANTHERPTHR315613-KETOACYL-COA SYNTHASEcoord: 18..111

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0040650.1Lag0040650.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0102756 very-long-chain 3-ketoacyl-CoA synthase activity
molecular_function GO:0016746 acyltransferase activity
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups