Lag0040637 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0040637
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Eukaryotic aspartyl protease family protein
Location	chr13: 6784700 .. 6787289 (-)
RNA-Seq Expression	Lag0040637
Synteny	Lag0040637

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTCTTTAAACTTCCCTCTTTTCTTCTCTTCTTTCTCCTTTTCCTCTTCTTCTCCTCCGCCGACCGACCTTCTCCGACCAAGTTTCAATACCTCGATGTCAAAGCAACCAAATTCGACTTTAACGATAGTCAGATTCTTCATACCCTTAGTTTCTTCGACGGTCACCAGTTAGCGACCGGTCGAAAATCCAACCATACTAAATTTAAGCTCAACCTTGTCCATCGGGATAAGCTATCCCACGTCCACGGCCGCCACCATGGCTTCGACGAGCGTATCAAAAGAGACGCCAAACGAGTCGCCACCCTCGTTCGCCGCCTATCGCGCGCTGCCTCCGCCGTACAAGATAGTAGTTACAAGGTGGCGAATTTTGCCACGGATGTCATTTCAGGCCAGGGGAAATTTTGGTTTTTTTTTTTTTTTTTATAACTTTTCTTTTAGGTTTTTTTATTATGTATTGTTGAAATGTTTGTGAATTTTTTTTTTAAATGATTTGGAATTGGAATTAGGAATGGAAGAGGGGAGTGGAGAGTATTTTGTTCGGATCGGAGTTGGGAGCCCGCCGAGGAGTCAGTATATGGTGATTGATTCCGGCAGTGACATTGTGTGGGTTCAATGCCAACCTTGTAGCCAATGCTACCAACAGTCCGATCCCGTTTTCGACCCGGCCGACTCCGCCTCGTTCACCGGTGTCTCCTGTGGCTCCGCCGTTTGTGACCGCCTTGAGAATGCCGGTTGTCACGCTGGACGGTGTCGGTATGAGGTGTCCTATGGGGATGGGTCTTACACTAAGGGCACTCTCGCCCTCGAAACTCTCACCATCGGTCAAGTGAGTACTATTAACTCAAAAACTTAAGCGAATAGATTATGGCATGTTTAATTATTTTAACATCCTTCTTTCTAAACTTATCTAAAAATTAGTATAAGGTTCAATAAGAAACTATTTCTGTTAAACTAGAAACTATTATTTTTGTGATTGTGTTTAATAAATCTATAAAATTTTCAACTTTGCATTTAATAAATTTGAGATAATATTTCATTATTATGTGACATGCTAAACATTTTTTTTATATAAGAAAAAATTAATTTTATAATTGAATTTTTTAAACTTTCTCTGCAGAAAAGTTTCATGCTACTATTCATTTTCTGACGCAATCCTGCAACCACTACTAGGAAATAAAATTTTTAAATATTTAATAAATGAAAAGTTGTCACGTGTCCTAGTTTCCAACCCAAATGGCGAATTTTCATTTTATTTCTCAAACTTTGAGGTTGGTTCTATTTTAGTTCTTAAATTTTCAAAATATCTATTTTGGTCCATAAACTTTTAAGTTTATTCTAATTTAATCTTTGAAATTTCAAAATTTCTATTTTAGTTCCTAAACTTTTTAAACATAACCCTTTATGGTCCTTGCTAGGTTACTTTTCTACCTAAATATGTTTTGGTCGAGTTTATCTCTAACTTATTTGGCTTGAAATCATTCTCATATTAACATGTTAATGCATATACTCGAAGTTACATCTTTAATTTGTTAAAATGAAATAAAATTGGGAACCAAAATAGTTATTTTTTGAAGTTCAATGACTAAAATAAACATTTTAAAAATTCGAGGACTAAAATAGAACAAACTTGAAAGTTGAGGAACTAAAATCAACCTTTGAAAATTTGAGGGACCAAAATAAAATACAGACTAAAATAGGATTTAAACATAATTTTAATTAAAGAGAAAATAATACTCCTTACCCACTAAATTGCCATTTATTAACAAGAAATCTTAAAGTTTTTTATTTTAGAACAAGAAAATTTAAAGTATATAGATAGATAAATTTTTATATTTAATCATCTATTAATATTTTTAATTAGGTCACGGTTCGTGACGTGGCAATCGGCTGCGGCCATACGAACCAAGGCATGTTCATCGGAGCCGCCGGGCTACTCGGTCTCGGTGGTGGCTCAATGTCATTCGTCGGCCAGCTTGGCGGTCAGACCGGCGGCGCATTCAGCTACTGTTTGGTGAGCCGAGGAACCGGCTCGACCGGAACATTAGAGTTCGGCCGCGGAGCAATGCCGGTTGGCGCCACGTGGATCTCCCTGGTCCGAAACCCACGCGCCCCAAGCTTCTACTACATCGGACTCGCCGGCGTCGGCGTCGGCGGCGTCAAAGTCCCGATACCGGAGGAAACTTTCCAGCTCTCCGAGTTCGGTGGCAACGGTGTGGTAATGGACACCGGCACCGCCGTGACGCGGCTGCCGACGGCGGCCTACGAGGCATTCCGCGATTCTTTCACGGCCCAAACCGGCAACCTCCCACGAGCGCCGGGAGTTTCGATCTTCGACACGTGTTACGATCTCAACGGGTTCGAGTCCGTACGGGTGCCAACGGTGTCGTTTTACTTCTCCGACGGGCCGGTGCTGACGCTGCCGGCGAAAAATTTTCTGATTCCGGTCGACGGCGCCGGAACTTTTTGCTTCGCTTTTGCGCCGTCGCCGTCGGGACTTTCCATAATCGGAAACATCCAGCAGGAAGGGATTCAGATTTCATTCGACGGGGCTAATGGGTTTGTGGGATTTGGCCCAAATGTTTGCTAA

mRNA sequence

ATGCTCTTTAAACTTCCCTCTTTTCTTCTCTTCTTTCTCCTTTTCCTCTTCTTCTCCTCCGCCGACCGACCTTCTCCGACCAAGTTTCAATACCTCGATGTCAAAGCAACCAAATTCGACTTTAACGATAGTCAGATTCTTCATACCCTTAGTTTCTTCGACGGTCACCAGTTAGCGACCGGTCGAAAATCCAACCATACTAAATTTAAGCTCAACCTTGTCCATCGGGATAAGCTATCCCACGTCCACGGCCGCCACCATGGCTTCGACGAGCGTATCAAAAGAGACGCCAAACGAGTCGCCACCCTCGTTCGCCGCCTATCGCGCGCTGCCTCCGCCGTACAAGATAGAATGGAAGAGGGGAGTGGAGAGTATTTTGTTCGGATCGGAGTTGGGAGCCCGCCGAGGAGTCAGTATATGGTGATTGATTCCGGCAGTGACATTGTGTGGGTTCAATGCCAACCTTGTAGCCAATGCTACCAACAGTCCGATCCCGTTTTCGACCCGGCCGACTCCGCCTCGTTCACCGGTGTCTCCTGTGGCTCCGCCGTTTGTGACCGCCTTGAGAATGCCGGTTGTCACGCTGGACGGTGTCGGTATGAGGTGTCCTATGGGGATGGGTCTTACACTAAGGGCACTCTCGCCCTCGAAACTCTCACCATCGGTCAAGTCACGGTTCGTGACGTGGCAATCGGCTGCGGCCATACGAACCAAGGCATGTTCATCGGAGCCGCCGGGCTACTCGGTCTCGGTGGTGGCTCAATGTCATTCGTCGGCCAGCTTGGCGGTCAGACCGGCGGCGCATTCAGCTACTGTTTGGTGAGCCGAGGAACCGGCTCGACCGGAACATTAGAGTTCGGCCGCGGAGCAATGCCGGTTGGCGCCACGTGGATCTCCCTGGTCCGAAACCCACGCGCCCCAAGCTTCTACTACATCGGACTCGCCGGCGTCGGCGTCGGCGGCGTCAAAGTCCCGATACCGGAGGAAACTTTCCAGCTCTCCGAGTTCGGTGGCAACGGTGTGGTAATGGACACCGGCACCGCCGTGACGCGGCTGCCGACGGCGGCCTACGAGGCATTCCGCGATTCTTTCACGGCCCAAACCGGCAACCTCCCACGAGCGCCGGGAGTTTCGATCTTCGACACGTGTTACGATCTCAACGGGTTCGAGTCCGTACGGGTGCCAACGGTGTCGTTTTACTTCTCCGACGGGCCGGTGCTGACGCTGCCGGCGAAAAATTTTCTGATTCCGGTCGACGGCGCCGGAACTTTTTGCTTCGCTTTTGCGCCGTCGCCGTCGGGACTTTCCATAATCGGAAACATCCAGCAGGAAGGGATTCAGATTTCATTCGACGGGGCTAATGGGTTTGTGGGATTTGGCCCAAATGTTTGCTAA

Coding sequence (CDS)

ATGCTCTTTAAACTTCCCTCTTTTCTTCTCTTCTTTCTCCTTTTCCTCTTCTTCTCCTCCGCCGACCGACCTTCTCCGACCAAGTTTCAATACCTCGATGTCAAAGCAACCAAATTCGACTTTAACGATAGTCAGATTCTTCATACCCTTAGTTTCTTCGACGGTCACCAGTTAGCGACCGGTCGAAAATCCAACCATACTAAATTTAAGCTCAACCTTGTCCATCGGGATAAGCTATCCCACGTCCACGGCCGCCACCATGGCTTCGACGAGCGTATCAAAAGAGACGCCAAACGAGTCGCCACCCTCGTTCGCCGCCTATCGCGCGCTGCCTCCGCCGTACAAGATAGAATGGAAGAGGGGAGTGGAGAGTATTTTGTTCGGATCGGAGTTGGGAGCCCGCCGAGGAGTCAGTATATGGTGATTGATTCCGGCAGTGACATTGTGTGGGTTCAATGCCAACCTTGTAGCCAATGCTACCAACAGTCCGATCCCGTTTTCGACCCGGCCGACTCCGCCTCGTTCACCGGTGTCTCCTGTGGCTCCGCCGTTTGTGACCGCCTTGAGAATGCCGGTTGTCACGCTGGACGGTGTCGGTATGAGGTGTCCTATGGGGATGGGTCTTACACTAAGGGCACTCTCGCCCTCGAAACTCTCACCATCGGTCAAGTCACGGTTCGTGACGTGGCAATCGGCTGCGGCCATACGAACCAAGGCATGTTCATCGGAGCCGCCGGGCTACTCGGTCTCGGTGGTGGCTCAATGTCATTCGTCGGCCAGCTTGGCGGTCAGACCGGCGGCGCATTCAGCTACTGTTTGGTGAGCCGAGGAACCGGCTCGACCGGAACATTAGAGTTCGGCCGCGGAGCAATGCCGGTTGGCGCCACGTGGATCTCCCTGGTCCGAAACCCACGCGCCCCAAGCTTCTACTACATCGGACTCGCCGGCGTCGGCGTCGGCGGCGTCAAAGTCCCGATACCGGAGGAAACTTTCCAGCTCTCCGAGTTCGGTGGCAACGGTGTGGTAATGGACACCGGCACCGCCGTGACGCGGCTGCCGACGGCGGCCTACGAGGCATTCCGCGATTCTTTCACGGCCCAAACCGGCAACCTCCCACGAGCGCCGGGAGTTTCGATCTTCGACACGTGTTACGATCTCAACGGGTTCGAGTCCGTACGGGTGCCAACGGTGTCGTTTTACTTCTCCGACGGGCCGGTGCTGACGCTGCCGGCGAAAAATTTTCTGATTCCGGTCGACGGCGCCGGAACTTTTTGCTTCGCTTTTGCGCCGTCGCCGTCGGGACTTTCCATAATCGGAAACATCCAGCAGGAAGGGATTCAGATTTCATTCGACGGGGCTAATGGGTTTGTGGGATTTGGCCCAAATGTTTGCTAA

Protein sequence

MLFKLPSFLLFFLLFLFFSSADRPSPTKFQYLDVKATKFDFNDSQILHTLSFFDGHQLATGRKSNHTKFKLNLVHRDKLSHVHGRHHGFDERIKRDAKRVATLVRRLSRAASAVQDRMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQSDPVFDPADSASFTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIGQVTVRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGTGSTGTLEFGRGAMPVGATWISLVRNPRAPSFYYIGLAGVGVGGVKVPIPEETFQLSEFGGNGVVMDTGTAVTRLPTAAYEAFRDSFTAQTGNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDGPVLTLPAKNFLIPVDGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNVC

Homology

[Close]

BLAST of Lag0040637 vs. NCBI nr
Match: KAG7012830.1 (Protein ASPARTIC PROTEASE IN GUARD CELL 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 808.1 bits (2086), Expect = 3.8e-230
Identity = 409/482 (84.85%), Postives = 432/482 (89.63%), Query Frame = 0


Query: 1   MLFKLP-SFLLFFLLFLFFSSADRPSPTKFQYLDVKATKFDFNDSQILHTLSFFDGHQLA 60
           ML KL  S LLFF + + F S  R SP +FQYL+VK++K D NDSQILHTL+F +GH+L 
Sbjct: 1   MLLKLHFSLLLFFSVSVVFGS--RSSPVEFQYLNVKSSKLDVNDSQILHTLNFSNGHRLV 60

Query: 61  TGRKSNHTKFKLNLVHRDKLSHVHGRHHGFDERIKRDAKRVATLVRRLSR---AASAVQD 120
           TGRKSNHTKFKL LVHRD+L H+HG+H GFDERIKRD KRVATLVRRLSR    +S VQD
Sbjct: 61  TGRKSNHTKFKLKLVHRDELPHIHGQHQGFDERIKRDVKRVATLVRRLSRYDTDSSGVQD 120

Query: 121 R--------------MEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQ 180
                          MEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPC QCYQQ
Sbjct: 121 NNYKVANFGTGVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCRQCYQQ 180

Query: 181 SDPVFDPADSASFTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIG 240
           SDPVF+PA+S S+TGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIG
Sbjct: 181 SDPVFNPAESTSYTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIG 240

Query: 241 QVTVRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGTGSTG 300
           +VTVRD+AIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRG GSTG
Sbjct: 241 EVTVRDMAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGIGSTG 300

Query: 301 TLEFGRGAMPVGATWISLVRNPRAPSFYYIGLAGVGVGGVKVPIPEETFQLSEFGGNGVV 360
           TLEFGRGAMPVGATWISLVRNP APSFYYIGLAGVGVGG+KVPIPEETFQL+E+G NGVV
Sbjct: 301 TLEFGRGAMPVGATWISLVRNPNAPSFYYIGLAGVGVGGIKVPIPEETFQLTEYGTNGVV 360

Query: 361 MDTGTAVTRLPTAAYEAFRDSFTAQTGNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS 420
           MDTGTAVTRLPTAAY AFRDSFTAQT NLPRAPGVSIFDTCY+LNGFESVRVPTVSFYFS
Sbjct: 361 MDTGTAVTRLPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYNLNGFESVRVPTVSFYFS 420

Query: 421 DGPVLTLPAKNFLIPVDGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN 465
           DGPVLTLPA NFLIPVD AGTFCFAFAPSPSGLSIIGNIQQ GIQISFDGANGFVGFGPN
Sbjct: 421 DGPVLTLPANNFLIPVDSAGTFCFAFAPSPSGLSIIGNIQQGGIQISFDGANGFVGFGPN 480

[Close]

BLAST of Lag0040637 vs. NCBI nr
Match: KAG6573756.1 (Protein ASPARTIC PROTEASE IN GUARD CELL 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 807.0 bits (2083), Expect = 8.6e-230
Identity = 408/482 (84.65%), Postives = 432/482 (89.63%), Query Frame = 0


Query: 1   MLFKLP-SFLLFFLLFLFFSSADRPSPTKFQYLDVKATKFDFNDSQILHTLSFFDGHQLA 60
           ML KL  S LLFF + + F S  R S  +FQYL++K++KFD NDSQILHTL+F +GH+L 
Sbjct: 1   MLLKLHFSLLLFFSVSVVFGS--RSSSVEFQYLNIKSSKFDVNDSQILHTLNFSNGHRLV 60

Query: 61  TGRKSNHTKFKLNLVHRDKLSHVHGRHHGFDERIKRDAKRVATLVRRLSR---AASAVQD 120
           TGRKSNHTKFKL LVHRD+L H+HG+H GFDERIKRD KRVATLVRRLSR    +S VQD
Sbjct: 61  TGRKSNHTKFKLKLVHRDELPHIHGQHQGFDERIKRDVKRVATLVRRLSRYDTDSSGVQD 120

Query: 121 R--------------MEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQ 180
                          MEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPC QCYQQ
Sbjct: 121 NNYKVANFGTGVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCRQCYQQ 180

Query: 181 SDPVFDPADSASFTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIG 240
           SDPVF+PA+S S+TGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIG
Sbjct: 181 SDPVFNPAESTSYTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIG 240

Query: 241 QVTVRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGTGSTG 300
           +VTVRD+AIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRG GSTG
Sbjct: 241 EVTVRDMAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGIGSTG 300

Query: 301 TLEFGRGAMPVGATWISLVRNPRAPSFYYIGLAGVGVGGVKVPIPEETFQLSEFGGNGVV 360
           TLEFGRGAMPVGATWISLVRNP APSFYYIGLAGVGVGG+KVPIPEETFQL+E+G NGVV
Sbjct: 301 TLEFGRGAMPVGATWISLVRNPNAPSFYYIGLAGVGVGGIKVPIPEETFQLTEYGTNGVV 360

Query: 361 MDTGTAVTRLPTAAYEAFRDSFTAQTGNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS 420
           MDTGTAVTRLPTAAY AFRDSFTAQT NLPRAPGVSIFDTCY+LNGFESVRVPTVSFYFS
Sbjct: 361 MDTGTAVTRLPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYNLNGFESVRVPTVSFYFS 420

Query: 421 DGPVLTLPAKNFLIPVDGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN 465
           DGPVLTLPA NFLIPVD AGTFCFAFAPSPSGLSIIGNIQQ GIQISFDGANGFVGFGPN
Sbjct: 421 DGPVLTLPANNFLIPVDSAGTFCFAFAPSPSGLSIIGNIQQGGIQISFDGANGFVGFGPN 480

[Close]

BLAST of Lag0040637 vs. NCBI nr
Match: XP_038893071.1 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Benincasa hispida])

HSP 1 Score: 806.2 bits (2081), Expect = 1.5e-229
Identity = 402/479 (83.92%), Postives = 426/479 (88.94%), Query Frame = 0


Query: 1   MLFKLPSFLLFFLLFLFFS-SADRPSPTKFQYLDVKATKFDFNDSQILHTLSFFDGHQLA 60
           M  K PS LLFFL FLFFS +A R SPTKFQYL+VKATK DFND QILHTL+F    +  
Sbjct: 1   MFLKQPSSLLFFLFFLFFSAAAGRSSPTKFQYLNVKATKLDFNDDQILHTLNFSHARRQV 60

Query: 61  TGRKSNHTKFKLNLVHRDKLSHVHGRHHGFDERIKRDAKRVATLVRRLSRAASAVQDR-- 120
           +G KS++  FKLNL+HRDKLSHVHG  HGF+ERIKRDA RVATLVRRLS    AVQD   
Sbjct: 61  SGHKSDNNTFKLNLLHRDKLSHVHGHRHGFNERIKRDAIRVATLVRRLSHGGGAVQDNKY 120

Query: 121 ------------MEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQSDP 180
                       MEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQC+PCS+CYQQSDP
Sbjct: 121 KVADFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRCYQQSDP 180

Query: 181 VFDPADSASFTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIGQVT 240
           VFDPADS+SF GVSC S VCDRLEN GC+AGRCRYEVSYGDGSYTKGTLALETLT+G+V 
Sbjct: 181 VFDPADSSSFAGVSCDSIVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVGEVM 240

Query: 241 VRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGTGSTGTLE 300
           +RDVAIGCGHTNQGMFIGAAGLLGLGGGSMSF+GQLGGQTGGAFSYCLVSRGTGSTGTLE
Sbjct: 241 IRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTLE 300

Query: 301 FGRGAMPVGATWISLVRNPRAPSFYYIGLAGVGVGGVKVPIPEETFQLSEFGGNGVVMDT 360
           FGRGA+PVGATWISL+RNPRAPSFYYIGLAG+GVGGV+V +PEETFQL+E+G NGVVMDT
Sbjct: 301 FGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTNGVVMDT 360

Query: 361 GTAVTRLPTAAYEAFRDSFTAQTGNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDGP 420
           GTAVTRLPTAAY A RDSFTAQT NLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDGP
Sbjct: 361 GTAVTRLPTAAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDGP 420

Query: 421 VLTLPAKNFLIPVDGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNVC 465
           VLTLPAKNFLIPVDG GTFC AFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNVC
Sbjct: 421 VLTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNVC 479

[Close]

BLAST of Lag0040637 vs. NCBI nr
Match: XP_023542067.1 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 804.3 bits (2076), Expect = 5.6e-229
Identity = 406/482 (84.23%), Postives = 433/482 (89.83%), Query Frame = 0


Query: 1   MLFKLP-SFLLFFLLFLFFSSADRPSPTKFQYLDVKATKFDFNDSQILHTLSFFDGHQLA 60
           ML KL  S LLFF + + F S  R SP +FQYL+VK++KFD +DSQIL TL+F +GH+L 
Sbjct: 1   MLLKLHFSLLLFFSVSVVFGS--RSSPVEFQYLNVKSSKFDVDDSQILRTLNFSNGHRLV 60

Query: 61  TGRKSNHTKFKLNLVHRDKLSHVHGRHHGFDERIKRDAKRVATLVRRLSR---AASAVQD 120
           TGRKSNHTKFKL LVHRD++ H+HG+H  FDERIKRD KRVATLVRRLSR    +S+VQD
Sbjct: 61  TGRKSNHTKFKLKLVHRDEIPHIHGQHQDFDERIKRDVKRVATLVRRLSRDDTDSSSVQD 120

Query: 121 R--------------MEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQ 180
                          MEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPC QCYQQ
Sbjct: 121 NGYKVANFGTGVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCRQCYQQ 180

Query: 181 SDPVFDPADSASFTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIG 240
           SDPVF+PA+S S+TGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIG
Sbjct: 181 SDPVFNPAESTSYTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIG 240

Query: 241 QVTVRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGTGSTG 300
           QVTVRD+AIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRG GSTG
Sbjct: 241 QVTVRDMAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGIGSTG 300

Query: 301 TLEFGRGAMPVGATWISLVRNPRAPSFYYIGLAGVGVGGVKVPIPEETFQLSEFGGNGVV 360
           TL+FGRGAMPVGATWISLVRNP APSFYYIGLAGVGVGG+KVPIPEETFQL+E+G NGVV
Sbjct: 301 TLDFGRGAMPVGATWISLVRNPNAPSFYYIGLAGVGVGGIKVPIPEETFQLTEYGTNGVV 360

Query: 361 MDTGTAVTRLPTAAYEAFRDSFTAQTGNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS 420
           MDTGTAVTRLPTAAY AFRDSFTAQT NLPRAPGVS+FDTCY+LNGFESVRVPTVSFYFS
Sbjct: 361 MDTGTAVTRLPTAAYVAFRDSFTAQTSNLPRAPGVSVFDTCYNLNGFESVRVPTVSFYFS 420

Query: 421 DGPVLTLPAKNFLIPVDGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN 465
           DGPVLTLPA NFLIPVDGAGTFCFAFAPSPSGLSIIGNIQQ GIQISFDGANGFVGFGPN
Sbjct: 421 DGPVLTLPANNFLIPVDGAGTFCFAFAPSPSGLSIIGNIQQGGIQISFDGANGFVGFGPN 480

[Close]

BLAST of Lag0040637 vs. NCBI nr
Match: XP_022945579.1 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucurbita moschata])

HSP 1 Score: 802.0 bits (2070), Expect = 2.8e-228
Identity = 405/482 (84.02%), Postives = 430/482 (89.21%), Query Frame = 0


Query: 1   MLFKLP-SFLLFFLLFLFFSSADRPSPTKFQYLDVKATKFDFNDSQILHTLSFFDGHQLA 60
           ML KL  S LLFF + + F S  R SP +FQYL+VK++KFD NDSQILH L+F +GH+L 
Sbjct: 1   MLLKLHFSLLLFFSVSVVFGS--RSSPVEFQYLNVKSSKFDANDSQILHILNFSNGHRLV 60

Query: 61  TGRKSNHTKFKLNLVHRDKLSHVHGRHHGFDERIKRDAKRVATLVRRLSR---AASAVQD 120
           TGRKSNHTKFKL LVHRD+L H+HG+H GFDERIKRD KRVATLVRRLSR    +S VQD
Sbjct: 61  TGRKSNHTKFKLKLVHRDELPHIHGQHQGFDERIKRDVKRVATLVRRLSRYDTDSSGVQD 120

Query: 121 R--------------MEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQ 180
                          MEEG+GEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPC QCYQQ
Sbjct: 121 NNYKVANFGTGVISGMEEGNGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCRQCYQQ 180

Query: 181 SDPVFDPADSASFTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIG 240
           SDPVF+PA+S S+TGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIG
Sbjct: 181 SDPVFNPAESTSYTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIG 240

Query: 241 QVTVRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGTGSTG 300
           +V + D+AIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRG GSTG
Sbjct: 241 EVMIHDMAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGIGSTG 300

Query: 301 TLEFGRGAMPVGATWISLVRNPRAPSFYYIGLAGVGVGGVKVPIPEETFQLSEFGGNGVV 360
           TLEFGRGAMPVGATWISLVRNP APSFYYIGLAGVGVGG+KVPIPEETFQL+E+G NGVV
Sbjct: 301 TLEFGRGAMPVGATWISLVRNPNAPSFYYIGLAGVGVGGIKVPIPEETFQLTEYGTNGVV 360

Query: 361 MDTGTAVTRLPTAAYEAFRDSFTAQTGNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS 420
           MDTGTAVTRLPTAAY AFRDSFTAQT NLPRAPGVSIFDTCY+LNGFESVRVPTVSFYFS
Sbjct: 361 MDTGTAVTRLPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYNLNGFESVRVPTVSFYFS 420

Query: 421 DGPVLTLPAKNFLIPVDGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN 465
           DGPVLTLPA NFLIPVD AGTFCFAFAPSPSGLSIIGNIQQ GIQISFDGANGFVGFGPN
Sbjct: 421 DGPVLTLPANNFLIPVDSAGTFCFAFAPSPSGLSIIGNIQQGGIQISFDGANGFVGFGPN 480

[Close]

BLAST of Lag0040637 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 8.8e-177
Identity = 317/483 (65.63%), Postives = 368/483 (76.19%), Query Frame = 0


Query: 5   LPSFLLFFLLFLFFSSADRPSPTKFQYLD-------VKATKFDFNDSQILHTLSFFDGHQ 64
           LP F  F  L L  SS+   S   FQ +D       V AT  DFN++       F D   
Sbjct: 3   LPLFFFFLHLHLHLSSSSSISFPDFQIIDVLQPPLTVTATLPDFNNTH------FSD--- 62

Query: 65  LATGRKSNHTKFKLNLVHRDKLSHV--HGRHHGFDERIKRDAKRVATLVRRLSRA----- 124
                  + +K+ L L+HRD+   V     HH    R++RD  RV+ ++RR+S       
Sbjct: 63  ------ESSSKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPSS 122

Query: 125 ---------ASAVQDRMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQ 184
                     S +   M++GSGEYFVRIGVGSPPR QYMVIDSGSD+VWVQCQPC  CY+
Sbjct: 123 DSRYEVNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYK 182

Query: 185 QSDPVFDPADSASFTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTI 244
           QSDPVFDPA S S+TGVSCGS+VCDR+EN+GCH+G CRYEV YGDGSYTKGTLALETLT 
Sbjct: 183 QSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTF 242

Query: 245 GQVTVRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGTGST 304
            +  VR+VA+GCGH N+GMFIGAAGLLG+GGGSMSFVGQL GQTGGAF YCLVSRGT ST
Sbjct: 243 AKTVVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDST 302

Query: 305 GTLEFGRGAMPVGATWISLVRNPRAPSFYYIGLAGVGVGGVKVPIPEETFQLSEFGGNGV 364
           G+L FGR A+PVGA+W+ LVRNPRAPSFYY+GL G+GVGGV++P+P+  F L+E G  GV
Sbjct: 303 GSLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGV 362

Query: 365 VMDTGTAVTRLPTAAYEAFRDSFTAQTGNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYF 424
           VMDTGTAVTRLPTAAY AFRD F +QT NLPRA GVSIFDTCYDL+GF SVRVPTVSFYF
Sbjct: 363 VMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYF 422

Query: 425 SDGPVLTLPAKNFLIPVDGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGP 465
           ++GPVLTLPA+NFL+PVD +GT+CFAFA SP+GLSIIGNIQQEGIQ+SFDGANGFVGFGP
Sbjct: 423 TEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGP 470

[Close]

BLAST of Lag0040637 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 1.1e-102
Identity = 219/483 (45.34%), Postives = 290/483 (60.04%), Query Frame = 0


Query: 9   LLFFLLFLFFSSADRPSPTKFQYLDVK------ATKFDF---NDSQILHTLSFFDGHQLA 68
           LLF L F F S     S   FQ L         A+   F   +DS+ L    F  G    
Sbjct: 8   LLFSLCFFFLSLPSFSSLPSFQTLFPNSHSLPCASPVSFQPDSDSESLLESEFESGSD-- 67

Query: 69  TGRKSNHTKFKLNLVHRDKLSHVHGRHHGFDERIKRDAKRV---ATLV-----RRLSRA- 128
                + +   LNL H D LS        F  R++RD++RV   ATL      R ++ A 
Sbjct: 68  ---SESSSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAP 127

Query: 129 -----ASAVQDRMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQSDP 188
                +S+V   + +GSGEYF R+GVG+P R  YMV+D+GSDIVW+QC PC +CY QSDP
Sbjct: 128 RPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDP 187

Query: 189 VFDPADSASFTGVSCGSAVCDRLENAGCHAGR--CRYEVSYGDGSYTKGTLALETLTIGQ 248
           +FDP  S ++  + C S  C RL++AGC+  R  C Y+VSYGDGS+T G  + ETLT  +
Sbjct: 188 IFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRR 247

Query: 249 VTVRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGTGS-TG 308
             V+ VA+GCGH N+G+F+GAAGLLGLG G +SF GQ G +    FSYCLV R   S   
Sbjct: 248 NRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPS 307

Query: 309 TLEFGRGAMPVGATWISLVRNPRAPSFYYIGLAGVGVGGVKVP-IPEETFQLSEFGGNGV 368
           ++ FG  A+   A +  L+ NP+  +FYY+GL G+ VGG +VP +    F+L + G  GV
Sbjct: 308 SVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGV 367

Query: 369 VMDTGTAVTRLPTAAYEAFRDSFTAQTGNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYF 428
           ++D+GT+VTRL   AY A RD+F      L RAP  S+FDTC+DL+    V+VPTV  +F
Sbjct: 368 IIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHF 427

Query: 429 SDGPVLTLPAKNFLIPVDGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGP 465
             G  ++LPA N+LIPVD  G FCFAFA +  GLSIIGNIQQ+G ++ +D A+  VGF P
Sbjct: 428 R-GADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAP 484

[Close]

BLAST of Lag0040637 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 1.1e-102
Identity = 185/400 (46.25%), Postives = 261/400 (65.25%), Query Frame = 0


Query: 92  RIKRDAKRVATLVRRLSRAASAVQDRME-------------------------EGSGEYF 151
           R++RD+ RVA +V ++  A   V DR +                         +GSGEYF
Sbjct: 105 RLERDSSRVAGIVAKIRFAVEGV-DRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYF 164

Query: 152 VRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQSDPVFDPADSASFTGVSCGSAVCD 211
            RIGVG+P +  Y+V+D+GSD+ W+QC+PC+ CYQQSDPVF+P  S+++  ++C +  C 
Sbjct: 165 SRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCS 224

Query: 212 RLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIGQV-TVRDVAIGCGHTNQGMFIGAA 271
            LE + C + +C Y+VSYGDGS+T G LA +T+T G    + +VA+GCGH N+G+F GAA
Sbjct: 225 LLETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAA 284

Query: 272 GLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGTGSTGTLEFGRGAMPVGATWISLVRNPR 331
           GLLGLGGG +S   Q+      +FSYCLV R +G + +L+F    +  G     L+RN +
Sbjct: 285 GLLGLGGGVLSITNQM---KATSFSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKK 344

Query: 332 APSFYYIGLAGVGVGGVKVPIPEETFQLSEFGGNGVVMDTGTAVTRLPTAAYEAFRDSFT 391
             +FYY+GL+G  VGG KV +P+  F +   G  GV++D GTAVTRL T AY + RD+F 
Sbjct: 345 IDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFL 404

Query: 392 AQTGNLPR-APGVSIFDTCYDLNGFESVRVPTVSFYFSDGPVLTLPAKNFLIPVDGAGTF 451
             T NL + +  +S+FDTCYD +   +V+VPTV+F+F+ G  L LPAKN+LIPVD +GTF
Sbjct: 405 KLTVNLKKGSSSISLFDTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTF 464

Query: 452 CFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNVC 465
           CFAFAP+ S LSIIGN+QQ+G +I++D +   +G   N C
Sbjct: 465 CFAFAPTSSSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

[Close]

BLAST of Lag0040637 vs. ExPASy Swiss-Prot
Match: Q8S9J6 (Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At5g10770 PE=2 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.1e-70
Identity = 161/427 (37.70%), Postives = 225/427 (52.69%), Query Frame = 0


Query: 63  KSNHTKFKLNLVHR----DKLSHVHGRHHGFDERIKRDAKRVATLVRRLSRAASA----- 122
           +++ TK  L++ HR     +L++         E ++ D  RV ++  +LS+  +      
Sbjct: 54  RASTTKSSLHVTHRHGTCSRLNNGKATSPDHVEILRLDQARVNSIHSKLSKKLATDHVSE 113

Query: 123 -------VQDRMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQ-CYQQSDP 182
                   +D    GSG Y V +G+G+P     ++ D+GSD+ W QCQPC + CY Q +P
Sbjct: 114 SKSTDLPAKDGSTLGSGNYIVTVGLGTPKNDLSLIFDTGSDLTWTQCQPCVRTCYDQKEP 173

Query: 183 VFDPADSASFTGVSCGSAVCDRLENA-----GCHAGRCRYEVSYGDGSYTKGTLALETLT 242
           +F+P+ S S+  VSC SA C  L +A      C A  C Y + YGD S++ G LA E  T
Sbjct: 174 IFNPSKSTSYYNVSCSSAACGSLSSATGNAGSCSASNCIYGIQYGDQSFSVGFLAKEKFT 233

Query: 243 IGQVTVRD-VAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGTG 302
           +    V D V  GCG  NQG+F G AGLLGLG   +SF  Q        FSYCL S    
Sbjct: 234 LTNSDVFDGVYFGCGENNQGLFTGVAGLLGLGRDKLSFPSQTATAYNKIFSYCLPS-SAS 293

Query: 303 STGTLEFGRGAMPVGATWISLVRNPRAPSFYYIGLAGVGVGGVKVPIPEETFQLSEFGGN 362
            TG L FG   +     +  +       SFY + +  + VGG K+PIP   F        
Sbjct: 294 YTGHLTFGSAGISRSVKFTPISTITDGTSFYGLNIVAITVGGQKLPIPSTVFSTP----- 353

Query: 363 GVVMDTGTAVTRLPTAAYEAFRDSFTAQTGNLPRAPGVSIFDTCYDLNGFESVRVPTVSF 422
           G ++D+GT +TRLP  AY A R SF A+    P   GVSI DTC+DL+GF++V +P V+F
Sbjct: 354 GALIDSGTVITRLPPKAYAALRSSFKAKMSKYPTTSGVSILDTCFDLSGFKTVTIPKVAF 413

Query: 423 YFSDGPVLTLPAKNFLIPVDGAGTFCFAFA--PSPSGLSIIGNIQQEGIQISFDGANGFV 465
            FS G V+ L +K  +  V      C AFA     S  +I GN+QQ+ +++ +DGA G V
Sbjct: 414 SFSGGAVVELGSKG-IFYVFKISQVCLAFAGNSDDSNAAIFGNVQQQTLEVVYDGAGGRV 473

[Close]

BLAST of Lag0040637 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 3.0e-68
Identity = 153/383 (39.95%), Postives = 218/383 (56.92%), Query Frame = 0


Query: 91  ERIKRDAKRVATLVRRLS---RAASAVQDRMEEGSGEYFVRIGVGSPPRSQYMVIDSGSD 150
           E IKR  KR    +R ++   +++S ++  +  G GEY + + +G+P  S   ++D+GSD
Sbjct: 59  ELIKRAIKRGERRMRSINAMLQSSSGIETPVYAGDGEYLMNVAIGTPDSSFSAIMDTGSD 118

Query: 151 IVWVQCQPCSQCYQQSDPVFDPADSASFTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDG 210
           ++W QC+PC+QC+ Q  P+F+P DS+SF+ + C S  C  L +  C+   C+Y   YGDG
Sbjct: 119 LIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQDLPSETCNNNECQYTYGYGDG 178

Query: 211 SYTKGTLALETLTIGQVTVRDVAIGCGHTNQGMFIG-AAGLLGLGGGSMSFVGQLGGQTG 270
           S T+G +A ET T    +V ++A GCG  NQG   G  AGL+G+G G +S   QLG    
Sbjct: 179 STTQGYMATETFTFETSSVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLG---V 238

Query: 271 GAFSYCLVSRGTGSTGTLEFGRGA--MPVGATWISLVRNPRAPSFYYIGLAGVGVGGVKV 330
           G FSYC+ S G+ S  TL  G  A  +P G+   +L+ +   P++YYI L G+ VGG  +
Sbjct: 239 GQFSYCMTSYGSSSPSTLALGSAASGVPEGSPSTTLIHSSLNPTYYYITLQGITVGGDNL 298

Query: 331 PIPEETFQLSEFGGNGVVMDTGTAVTRLPTAAYEAFRDSFTAQTGNLPRA-PGVSIFDTC 390
            IP  TFQL + G  G+++D+GT +T LP  AY A   +FT Q  NLP      S   TC
Sbjct: 299 GIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQI-NLPTVDESSSGLSTC 358

Query: 391 YDL-NGFESVRVPTVSFYFSDGPVLTLPAKNFLIPVDGAGTFCFAFAPSPS-GLSIIGNI 450
           +   +   +V+VP +S  F DG VL L  +N LI     G  C A   S   G+SI GNI
Sbjct: 359 FQQPSDGSTVQVPEISMQF-DGGVLNLGEQNILIS-PAEGVICLAMGSSSQLGISIFGNI 418

Query: 451 QQEGIQISFDGANGFVGFGPNVC 465
           QQ+  Q+ +D  N  V F P  C
Sbjct: 419 QQQETQVLYDLQNLAVSFVPTQC 435

[Close]

BLAST of Lag0040637 vs. ExPASy TrEMBL
Match: A0A6J1G1D0 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita moschata OX=3662 GN=LOC111449773 PE=4 SV=1)

HSP 1 Score: 802.0 bits (2070), Expect = 1.3e-228
Identity = 405/482 (84.02%), Postives = 430/482 (89.21%), Query Frame = 0


Query: 1   MLFKLP-SFLLFFLLFLFFSSADRPSPTKFQYLDVKATKFDFNDSQILHTLSFFDGHQLA 60
           ML KL  S LLFF + + F S  R SP +FQYL+VK++KFD NDSQILH L+F +GH+L 
Sbjct: 1   MLLKLHFSLLLFFSVSVVFGS--RSSPVEFQYLNVKSSKFDANDSQILHILNFSNGHRLV 60

Query: 61  TGRKSNHTKFKLNLVHRDKLSHVHGRHHGFDERIKRDAKRVATLVRRLSR---AASAVQD 120
           TGRKSNHTKFKL LVHRD+L H+HG+H GFDERIKRD KRVATLVRRLSR    +S VQD
Sbjct: 61  TGRKSNHTKFKLKLVHRDELPHIHGQHQGFDERIKRDVKRVATLVRRLSRYDTDSSGVQD 120

Query: 121 R--------------MEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQ 180
                          MEEG+GEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPC QCYQQ
Sbjct: 121 NNYKVANFGTGVISGMEEGNGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCRQCYQQ 180

Query: 181 SDPVFDPADSASFTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIG 240
           SDPVF+PA+S S+TGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIG
Sbjct: 181 SDPVFNPAESTSYTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIG 240

Query: 241 QVTVRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGTGSTG 300
           +V + D+AIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRG GSTG
Sbjct: 241 EVMIHDMAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGIGSTG 300

Query: 301 TLEFGRGAMPVGATWISLVRNPRAPSFYYIGLAGVGVGGVKVPIPEETFQLSEFGGNGVV 360
           TLEFGRGAMPVGATWISLVRNP APSFYYIGLAGVGVGG+KVPIPEETFQL+E+G NGVV
Sbjct: 301 TLEFGRGAMPVGATWISLVRNPNAPSFYYIGLAGVGVGGIKVPIPEETFQLTEYGTNGVV 360

Query: 361 MDTGTAVTRLPTAAYEAFRDSFTAQTGNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS 420
           MDTGTAVTRLPTAAY AFRDSFTAQT NLPRAPGVSIFDTCY+LNGFESVRVPTVSFYFS
Sbjct: 361 MDTGTAVTRLPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYNLNGFESVRVPTVSFYFS 420

Query: 421 DGPVLTLPAKNFLIPVDGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN 465
           DGPVLTLPA NFLIPVD AGTFCFAFAPSPSGLSIIGNIQQ GIQISFDGANGFVGFGPN
Sbjct: 421 DGPVLTLPANNFLIPVDSAGTFCFAFAPSPSGLSIIGNIQQGGIQISFDGANGFVGFGPN 480

[Close]

BLAST of Lag0040637 vs. ExPASy TrEMBL
Match: A0A6J1HQG0 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita maxima OX=3661 GN=LOC111466434 PE=4 SV=1)

HSP 1 Score: 799.3 bits (2063), Expect = 8.7e-228
Identity = 403/482 (83.61%), Postives = 428/482 (88.80%), Query Frame = 0


Query: 1   MLFKLP-SFLLFFLLFLFFSSADRPSPTKFQYLDVKATKFDFNDSQILHTLSFFDGHQLA 60
           ML KL  S LLFF + +   S  R S  +FQYL+VK+ KFD NDSQ+LHTL+F +GH+L 
Sbjct: 1   MLLKLHFSLLLFFSVSVVLGS--RSSSVEFQYLNVKSNKFDVNDSQLLHTLNFSNGHRLV 60

Query: 61  TGRKSNHTKFKLNLVHRDKLSHVHGRHHGFDERIKRDAKRVATLVRRLSR---AASAVQD 120
           TGRKSNHTKFKL LVHRDKL H+HG+H GFDERIKRD KRVATLVRRLSR     S V+D
Sbjct: 61  TGRKSNHTKFKLKLVHRDKLPHIHGQHQGFDERIKRDVKRVATLVRRLSRDDTTTSVVED 120

Query: 121 R--------------MEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQ 180
                          MEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPC QCYQQ
Sbjct: 121 NSYKVANFGTGVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCRQCYQQ 180

Query: 181 SDPVFDPADSASFTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIG 240
           SDPVF+PA+S S+TGVSCGSA+CDRLENAGCH+GRCRYEVSYGDGSYTKGTLALETLTIG
Sbjct: 181 SDPVFNPAESTSYTGVSCGSAICDRLENAGCHSGRCRYEVSYGDGSYTKGTLALETLTIG 240

Query: 241 QVTVRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGTGSTG 300
           +VTVRD+AIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRG GSTG
Sbjct: 241 EVTVRDMAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGIGSTG 300

Query: 301 TLEFGRGAMPVGATWISLVRNPRAPSFYYIGLAGVGVGGVKVPIPEETFQLSEFGGNGVV 360
           TL+FGRGAMPVGATWISLVRNP APSFYYIGLAGVGVGG+KVPIPEETFQL+E+G NGVV
Sbjct: 301 TLDFGRGAMPVGATWISLVRNPNAPSFYYIGLAGVGVGGIKVPIPEETFQLTEYGTNGVV 360

Query: 361 MDTGTAVTRLPTAAYEAFRDSFTAQTGNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS 420
           MDTGTAVTRLPTAAY AFRDSFTA T NLPRAPGVSIFDTCY+LNGFESVRVPTVSFYFS
Sbjct: 361 MDTGTAVTRLPTAAYVAFRDSFTALTSNLPRAPGVSIFDTCYNLNGFESVRVPTVSFYFS 420

Query: 421 DGPVLTLPAKNFLIPVDGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN 465
           DGPVLTLPA NFLIPVD AGTFCFAFAPSPSGLSIIGNIQQ GIQISFDGANGFVGFGPN
Sbjct: 421 DGPVLTLPANNFLIPVDSAGTFCFAFAPSPSGLSIIGNIQQGGIQISFDGANGFVGFGPN 480

[Close]

BLAST of Lag0040637 vs. ExPASy TrEMBL
Match: A0A5A7SVY2 (Protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00740 PE=4 SV=1)

HSP 1 Score: 795.8 bits (2054), Expect = 9.6e-227
Identity = 396/467 (84.80%), Postives = 427/467 (91.43%), Query Frame = 0


Query: 1   MLFK-LPSFLLFFL-LFLFFSSADRP-SPTKFQYLDVKATKFDFNDSQILHTLSFFDGHQ 60
           M FK L S LLFFL + +  ++A RP SPTKFQYL+VKATK DFND QILHTL+F D H+
Sbjct: 1   MFFKQLISSLLFFLAVLVVVAAAARPSSPTKFQYLNVKATKLDFNDGQILHTLNFSDAHR 60

Query: 61  LATGRKSNHTKFKLNLVHRDKLSHVHGRHHGFDERIKRDAKRVATLVRRLSRAASAVQDR 120
             +G KS++  FKLNL+HRDKLSHVHG   GF++R+KRDA RVATLVRRLS  A+AV+D 
Sbjct: 61  QVSGHKSHNDTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGAAAVEDS 120

Query: 121 MEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQSDPVFDPADSASFTG 180
             +GSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQC+PCS+CYQQSDPVFDPADS+SF G
Sbjct: 121 RFKGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRCYQQSDPVFDPADSSSFAG 180

Query: 181 VSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIGQVTVRDVAIGCGHTN 240
           VSCGS VCDRLEN GC+AGRCRYEVSYGDGSYTKGTLALETLT+GQV +RDVAIGCGHTN
Sbjct: 181 VSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVGQVMIRDVAIGCGHTN 240

Query: 241 QGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGTGSTGTLEFGRGAMPVGATW 300
           QGMFIGAAGLLGLGGGSMSF+GQLGGQTGGAFSYCLVSRGTGSTGTLEFGRGA+PVGATW
Sbjct: 241 QGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTLEFGRGALPVGATW 300

Query: 301 ISLVRNPRAPSFYYIGLAGVGVGGVKVPIPEETFQLSEFGGNGVVMDTGTAVTRLPTAAY 360
           ISL+RNPRAPSFYYIGLAG+GVGGV+V IPEETFQL+EFG NGVVMDTGTAVTRLPT+AY
Sbjct: 301 ISLIRNPRAPSFYYIGLAGIGVGGVRVSIPEETFQLTEFGTNGVVMDTGTAVTRLPTSAY 360

Query: 361 EAFRDSFTAQTGNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDGPVLTLPAKNFLIP 420
            A RDSFTAQT NLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDGP LTLPAKNFLIP
Sbjct: 361 VALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDGPALTLPAKNFLIP 420

Query: 421 VDGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNVC 465
           VDG GTFC AFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN+C
Sbjct: 421 VDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 467

[Close]

BLAST of Lag0040637 vs. ExPASy TrEMBL
Match: A0A1S3BEG7 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucumis melo OX=3656 GN=LOC103488783 PE=4 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 6.2e-226
Identity = 399/481 (82.95%), Postives = 429/481 (89.19%), Query Frame = 0


Query: 1   MLFK-LPSFLLFFL-LFLFFSSADRP-SPTKFQYLDVKATKFDFNDSQILHTLSFFDGHQ 60
           M FK L S LLFFL + +  ++A RP SPTKFQYL+VKATK DFND QILHTL+F D H+
Sbjct: 1   MFFKQLISSLLFFLAVLVVVAAAARPSSPTKFQYLNVKATKLDFNDGQILHTLNFSDAHR 60

Query: 61  LATGRKSNHTKFKLNLVHRDKLSHVHGRHHGFDERIKRDAKRVATLVRRLSRAASAVQDR 120
             +G KS++  FKLNL+HRDKLSHVHG   GF++R+KRDA RVATLVRRLS  A+AV+D 
Sbjct: 61  QVSGHKSHNDTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGAAAVEDS 120

Query: 121 --------------MEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQS 180
                         MEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQC+PCS+CYQQS
Sbjct: 121 RFKVANFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRCYQQS 180

Query: 181 DPVFDPADSASFTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIGQ 240
           DPVFDPADS+SF GVSCGS VCDRLEN GC+AGRCRYEVSYGDGSYTKGTLALETLT+GQ
Sbjct: 181 DPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVGQ 240

Query: 241 VTVRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGTGSTGT 300
           V +RDVAIGCGHTNQGMFIGAAGLLGLGGGSMSF+GQLGGQTGGAFSYCLVSRGTGSTGT
Sbjct: 241 VMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGT 300

Query: 301 LEFGRGAMPVGATWISLVRNPRAPSFYYIGLAGVGVGGVKVPIPEETFQLSEFGGNGVVM 360
           LEFGRGA+PVGATWISL+RNPRAPSFYYIGLAG+GVGGV+V IPEETFQL+EFG NGVVM
Sbjct: 301 LEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSIPEETFQLTEFGTNGVVM 360

Query: 361 DTGTAVTRLPTAAYEAFRDSFTAQTGNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSD 420
           DTGTAVTRLPT+AY A RDSFTAQT NLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSD
Sbjct: 361 DTGTAVTRLPTSAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSD 420

Query: 421 GPVLTLPAKNFLIPVDGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNV 465
           GP LTLPAKNFLIPVDG GTFC AFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN+
Sbjct: 421 GPALTLPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNI 480

[Close]

BLAST of Lag0040637 vs. ExPASy TrEMBL
Match: A0A0A0KPR4 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G579060 PE=4 SV=1)

HSP 1 Score: 775.8 bits (2002), Expect = 1.0e-220
Identity = 391/482 (81.12%), Postives = 424/482 (87.97%), Query Frame = 0


Query: 1   MLFK-LPSFLLFFLLFLF--FSSADRPSPTKFQYLDVKATKFDFNDSQILHTLSFFDGHQ 60
           M FK L S LLF L+ +    ++A   S TKFQYL+VKATK DFND QILH L+F DGH+
Sbjct: 1   MFFKQLFSSLLFLLVVIVGAAAAARLSSSTKFQYLNVKATKLDFNDGQILHALNFSDGHR 60

Query: 61  LATGRKSNHTKFKLNLVHRDKLSHVHGRHHGFDERIKRDAKRVATLVRRLSRAA-SAVQD 120
             +G KS++  FKLNL+HRDKLSHVHG   GF++R+KRDA RVATLVRRLS  A +AV+D
Sbjct: 61  QVSGYKSDNNTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSHGAPAAVKD 120

Query: 121 R--------------MEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQ 180
                          ME GSGEYFVRIGVGSPPR+QYMVIDSGSDIVWVQC+PCS+CYQQ
Sbjct: 121 SRYKVANFATDVISGMEAGSGEYFVRIGVGSPPRNQYMVIDSGSDIVWVQCKPCSRCYQQ 180

Query: 181 SDPVFDPADSASFTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIG 240
           SDPVFDPADS+SF GVSCGS VCDRLEN GC+AGRCRYEVSYGDGSYTKGTLALETLT+G
Sbjct: 181 SDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVG 240

Query: 241 QVTVRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGTGSTG 300
           QV +RDVAIGCGHTNQGMFIGAAGLLGLGGGSMSF+GQLGGQTGGAFSYCLVSRGTGSTG
Sbjct: 241 QVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTG 300

Query: 301 TLEFGRGAMPVGATWISLVRNPRAPSFYYIGLAGVGVGGVKVPIPEETFQLSEFGGNGVV 360
            LEFGRGA+PVGATWISL+RNPRAPSFYYIGLAG+GVGGV+V +PEETFQL+E+G NGVV
Sbjct: 301 ALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTNGVV 360

Query: 361 MDTGTAVTRLPTAAYEAFRDSFTAQTGNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS 420
           MDTGTAVTR PTAAY AFRDSFTAQT NLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS
Sbjct: 361 MDTGTAVTRFPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFS 420

Query: 421 DGPVLTLPAKNFLIPVDGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN 465
           DGPVLTLPA+NFLIPVDG GTFC AFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN
Sbjct: 421 DGPVLTLPARNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPN 480

[Close]

BLAST of Lag0040637 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 621.3 bits (1601), Expect = 6.2e-178
Identity = 317/483 (65.63%), Postives = 368/483 (76.19%), Query Frame = 0


Query: 5   LPSFLLFFLLFLFFSSADRPSPTKFQYLD-------VKATKFDFNDSQILHTLSFFDGHQ 64
           LP F  F  L L  SS+   S   FQ +D       V AT  DFN++       F D   
Sbjct: 3   LPLFFFFLHLHLHLSSSSSISFPDFQIIDVLQPPLTVTATLPDFNNTH------FSD--- 62

Query: 65  LATGRKSNHTKFKLNLVHRDKLSHV--HGRHHGFDERIKRDAKRVATLVRRLSRA----- 124
                  + +K+ L L+HRD+   V     HH    R++RD  RV+ ++RR+S       
Sbjct: 63  ------ESSSKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPSS 122

Query: 125 ---------ASAVQDRMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQ 184
                     S +   M++GSGEYFVRIGVGSPPR QYMVIDSGSD+VWVQCQPC  CY+
Sbjct: 123 DSRYEVNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYK 182

Query: 185 QSDPVFDPADSASFTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTI 244
           QSDPVFDPA S S+TGVSCGS+VCDR+EN+GCH+G CRYEV YGDGSYTKGTLALETLT 
Sbjct: 183 QSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTF 242

Query: 245 GQVTVRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGTGST 304
            +  VR+VA+GCGH N+GMFIGAAGLLG+GGGSMSFVGQL GQTGGAF YCLVSRGT ST
Sbjct: 243 AKTVVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDST 302

Query: 305 GTLEFGRGAMPVGATWISLVRNPRAPSFYYIGLAGVGVGGVKVPIPEETFQLSEFGGNGV 364
           G+L FGR A+PVGA+W+ LVRNPRAPSFYY+GL G+GVGGV++P+P+  F L+E G  GV
Sbjct: 303 GSLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGV 362

Query: 365 VMDTGTAVTRLPTAAYEAFRDSFTAQTGNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYF 424
           VMDTGTAVTRLPTAAY AFRD F +QT NLPRA GVSIFDTCYDL+GF SVRVPTVSFYF
Sbjct: 363 VMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYF 422

Query: 425 SDGPVLTLPAKNFLIPVDGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGP 465
           ++GPVLTLPA+NFL+PVD +GT+CFAFA SP+GLSIIGNIQQEGIQ+SFDGANGFVGFGP
Sbjct: 423 TEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGP 470

[Close]

BLAST of Lag0040637 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 386.7 bits (992), Expect = 2.6e-107
Identity = 206/490 (42.04%), Postives = 292/490 (59.59%), Query Frame = 0


Query: 6   PSFLLFFLLFLFFSSADRPSPTKFQYLDVKATKFDFNDSQILHTLSFFDGHQLATGRKSN 65
           P++  FF  F+FF ++     ++        T    N +  +H   +    +L    +  
Sbjct: 3   PNYSFFF--FIFFLTSHSSVFSRILPETSTTTTSILNVADSIHRTKYTSSFRLNQQEEQT 62

Query: 66  H---TKFKLNLVHRDKLSHVHGRHHGFDE-----RIKRDAKRVATLVRRLSRAASAVQ-- 125
           H   + F L L  R     V G  H   +     R+ RD  RV +L+ RL  A + +   
Sbjct: 63  HSASSSFSLQLHSR---VSVRGTEHSDYKSLTLARLNRDTARVKSLITRLDLAINNISKA 122

Query: 126 ---------------------DRMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQ 185
                                    +GSGEYF R+G+G P R  YMV+D+GSD+ W+QC 
Sbjct: 123 DLKPISTMYTTEEQDIEAPLISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSDVNWLQCT 182

Query: 186 PCSQCYQQSDPVFDPADSASFTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTL 245
           PC+ CY Q++P+F+P+ S+S+  +SC +  C+ LE + C    C YEVSYGDGSYT G  
Sbjct: 183 PCADCYHQTEPIFEPSSSSSYEPLSCDTPQCNALEVSECRNATCLYEVSYGDGSYTVGDF 242

Query: 246 ALETLTIGQVTVRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLV 305
           A ETLTIG   V++VA+GCGH+N+G+F+GAAGLLGLGGG ++   QL      +FSYCLV
Sbjct: 243 ATETLTIGSTLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTT---SFSYCLV 302

Query: 306 SRGTGSTGTLEFGRGAMPVGATWISLVRNPRAPSFYYIGLAGVGVGGVKVPIPEETFQLS 365
            R + S  T++FG    P  A    L+RN +  +FYY+GL G+ VGG  + IP+ +F++ 
Sbjct: 303 DRDSDSASTVDFGTSLSP-DAVVAPLLRNHQLDTFYYLGLTGISVGGELLQIPQSSFEMD 362

Query: 366 EFGGNGVVMDTGTAVTRLPTAAYEAFRDSFTAQTGNLPRAPGVSIFDTCYDLNGFESVRV 425
           E G  G+++D+GTAVTRL T  Y + RDSF   T +L +A GV++FDTCY+L+   +V V
Sbjct: 363 ESGSGGIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKTTVEV 422

Query: 426 PTVSFYFSDGPVLTLPAKNFLIPVDGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGAN 465
           PTV+F+F  G +L LPAKN++IPVD  GTFC AFAP+ S L+IIGN+QQ+G +++FD AN
Sbjct: 423 PTVAFHFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTRVTFDLAN 482

[Close]

BLAST of Lag0040637 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 375.2 bits (962), Expect = 7.8e-104
Identity = 219/483 (45.34%), Postives = 290/483 (60.04%), Query Frame = 0


Query: 9   LLFFLLFLFFSSADRPSPTKFQYLDVK------ATKFDF---NDSQILHTLSFFDGHQLA 68
           LLF L F F S     S   FQ L         A+   F   +DS+ L    F  G    
Sbjct: 8   LLFSLCFFFLSLPSFSSLPSFQTLFPNSHSLPCASPVSFQPDSDSESLLESEFESGSD-- 67

Query: 69  TGRKSNHTKFKLNLVHRDKLSHVHGRHHGFDERIKRDAKRV---ATLV-----RRLSRA- 128
                + +   LNL H D LS        F  R++RD++RV   ATL      R ++ A 
Sbjct: 68  ---SESSSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAP 127

Query: 129 -----ASAVQDRMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQSDP 188
                +S+V   + +GSGEYF R+GVG+P R  YMV+D+GSDIVW+QC PC +CY QSDP
Sbjct: 128 RPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDP 187

Query: 189 VFDPADSASFTGVSCGSAVCDRLENAGCHAGR--CRYEVSYGDGSYTKGTLALETLTIGQ 248
           +FDP  S ++  + C S  C RL++AGC+  R  C Y+VSYGDGS+T G  + ETLT  +
Sbjct: 188 IFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRR 247

Query: 249 VTVRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGTGS-TG 308
             V+ VA+GCGH N+G+F+GAAGLLGLG G +SF GQ G +    FSYCLV R   S   
Sbjct: 248 NRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPS 307

Query: 309 TLEFGRGAMPVGATWISLVRNPRAPSFYYIGLAGVGVGGVKVP-IPEETFQLSEFGGNGV 368
           ++ FG  A+   A +  L+ NP+  +FYY+GL G+ VGG +VP +    F+L + G  GV
Sbjct: 308 SVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGV 367

Query: 369 VMDTGTAVTRLPTAAYEAFRDSFTAQTGNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYF 428
           ++D+GT+VTRL   AY A RD+F      L RAP  S+FDTC+DL+    V+VPTV  +F
Sbjct: 368 IIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHF 427

Query: 429 SDGPVLTLPAKNFLIPVDGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGP 465
             G  ++LPA N+LIPVD  G FCFAFA +  GLSIIGNIQQ+G ++ +D A+  VGF P
Sbjct: 428 R-GADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAP 484

[Close]

BLAST of Lag0040637 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 375.2 bits (962), Expect = 7.8e-104
Identity = 185/400 (46.25%), Postives = 261/400 (65.25%), Query Frame = 0


Query: 92  RIKRDAKRVATLVRRLSRAASAVQDRME-------------------------EGSGEYF 151
           R++RD+ RVA +V ++  A   V DR +                         +GSGEYF
Sbjct: 105 RLERDSSRVAGIVAKIRFAVEGV-DRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYF 164

Query: 152 VRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQSDPVFDPADSASFTGVSCGSAVCD 211
            RIGVG+P +  Y+V+D+GSD+ W+QC+PC+ CYQQSDPVF+P  S+++  ++C +  C 
Sbjct: 165 SRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCS 224

Query: 212 RLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIGQV-TVRDVAIGCGHTNQGMFIGAA 271
            LE + C + +C Y+VSYGDGS+T G LA +T+T G    + +VA+GCGH N+G+F GAA
Sbjct: 225 LLETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAA 284

Query: 272 GLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGTGSTGTLEFGRGAMPVGATWISLVRNPR 331
           GLLGLGGG +S   Q+      +FSYCLV R +G + +L+F    +  G     L+RN +
Sbjct: 285 GLLGLGGGVLSITNQM---KATSFSYCLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKK 344

Query: 332 APSFYYIGLAGVGVGGVKVPIPEETFQLSEFGGNGVVMDTGTAVTRLPTAAYEAFRDSFT 391
             +FYY+GL+G  VGG KV +P+  F +   G  GV++D GTAVTRL T AY + RD+F 
Sbjct: 345 IDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFL 404

Query: 392 AQTGNLPR-APGVSIFDTCYDLNGFESVRVPTVSFYFSDGPVLTLPAKNFLIPVDGAGTF 451
             T NL + +  +S+FDTCYD +   +V+VPTV+F+F+ G  L LPAKN+LIPVD +GTF
Sbjct: 405 KLTVNLKKGSSSISLFDTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTF 464

Query: 452 CFAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNVC 465
           CFAFAP+ S LSIIGN+QQ+G +I++D +   +G   N C
Sbjct: 465 CFAFAPTSSSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

[Close]

BLAST of Lag0040637 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 350.9 bits (899), Expect = 1.6e-96
Identity = 211/487 (43.33%), Postives = 290/487 (59.55%), Query Frame = 0


Query: 7   SFLLFFLLFLFFSSADRPSPTKFQYLDVKATKFDFNDSQILHTLSFFDGHQLATGRKS-N 66
           +F +F +LF F SSA     +++Q L V       N      TLS+ +   L     S +
Sbjct: 10  AFSVFAVLF-FTSSAS----SQYQTLVV-------NTLPSSATLSWPESESLTDESLSES 69

Query: 67  HTKFKLNLVHRDKLSHVHGRHHG--FDERIKRDAKRVATL------------VRRLSRAA 126
            T   ++L H D LS          F+ R++RD+ RV ++             +R  R A
Sbjct: 70  TTSLSVHLSHVDALSSFSDASPADLFNLRLQRDSLRVKSITSLAAVSTGRNATKRTPRTA 129

Query: 127 ----SAVQDRMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCSQCYQQSDPVF 186
                AV   + +GSGEYF+R+GVG+P  + YMV+D+GSD+VW+QC PC  CY Q+D +F
Sbjct: 130 GGFSGAVISGLSQGSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSPCKACYNQTDAIF 189

Query: 187 DPADSASFTGVSCGSAVCDRLENAG-CHAGR---CRYEVSYGDGSYTKGTLALETLTIGQ 246
           DP  S +F  V CGS +C RL+++  C   R   C Y+VSYGDGS+T+G  + ETLT   
Sbjct: 190 DPKKSKTFATVPCGSRLCRRLDDSSECVTRRSKTCLYQVSYGDGSFTEGDFSTETLTFHG 249

Query: 247 VTVRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSR-GTGST- 306
             V  V +GCGH N+G+F+GAAGLLGLG G +SF  Q   +  G FSYCLV R  +GS+ 
Sbjct: 250 ARVDHVPLGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTKNRYNGKFSYCLVDRTSSGSSS 309

Query: 307 ---GTLEFGRGAMPVGATWISLVRNPRAPSFYYIGLAGVGVGGVKVP-IPEETFQLSEFG 366
               T+ FG  A+P  + +  L+ NP+  +FYY+ L G+ VGG +VP + E  F+L   G
Sbjct: 310 KPPSTIVFGNAAVPKTSVFTPLLTNPKLDTFYYLQLLGISVGGSRVPGVSESQFKLDATG 369

Query: 367 GNGVVMDTGTAVTRLPTAAYEAFRDSFTAQTGNLPRAPGVSIFDTCYDLNGFESVRVPTV 426
             GV++D+GT+VTRL   AY A RD+F      L RAP  S+FDTC+DL+G  +V+VPTV
Sbjct: 370 NGGVIIDSGTSVTRLTQPAYVALRDAFRLGATKLKRAPSYSLFDTCFDLSGMTTVKVPTV 429

Query: 427 SFYFSDGPVLTLPAKNFLIPVDGAGTFCFAFAPSPSGLSIIGNIQQEGIQISFDGANGFV 465
            F+F  G V +LPA N+LIPV+  G FCFAFA +   LSIIGNIQQ+G ++++D     V
Sbjct: 430 VFHFGGGEV-SLPASNYLIPVNTEGRFCFAFAGTMGSLSIIGNIQQQGFRVAYDLVGSRV 483

The following BLAST results are available for this feature:

BLAST of Lag0040637 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7012830.1	3.8e-230	84.85	Protein ASPARTIC PROTEASE IN GUARD CELL 2, partial [Cucurbita argyrosperma subsp...	[more]
KAG6573756.1	8.6e-230	84.65	Protein ASPARTIC PROTEASE IN GUARD CELL 2, partial [Cucurbita argyrosperma subsp...	[more]
XP_038893071.1	1.5e-229	83.92	protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Benincasa hispida]	[more]
XP_023542067.1	5.6e-229	84.23	protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucurbita pepo subsp. pepo]	[more]
XP_022945579.1	2.8e-228	84.02	protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucurbita moschata]	[more]

BLAST of Lag0040637 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LHE3	8.8e-177	65.63	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LNJ3	1.1e-102	45.34	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q9LS40	1.1e-102	46.25	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q8S9J6	1.1e-70	37.70	Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At...	[more]
Q766C2	3.0e-68	39.95	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]

BLAST of Lag0040637 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G1D0	1.3e-228	84.02	protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1HQG0	8.7e-228	83.61	protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A5A7SVY2	9.6e-227	84.80	Protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucumis melo var. makuwa OX=11...	[more]
A0A1S3BEG7	6.2e-226	82.95	protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucumis melo OX=3656 GN=LOC103...	[more]
A0A0A0KPR4	1.0e-220	81.12	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G57906...	[more]

BLAST of Lag0040637 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G20015.1	6.2e-178	65.63	Eukaryotic aspartyl protease family protein	[more]
AT1G25510.1	2.6e-107	42.04	Eukaryotic aspartyl protease family protein	[more]
AT1G01300.1	7.8e-104	45.34	Eukaryotic aspartyl protease family protein	[more]
AT3G18490.1	7.8e-104	46.25	Eukaryotic aspartyl protease family protein	[more]
AT3G61820.1	1.6e-96	43.33	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 125..287 e-value: 3.8E-49 score: 167.3
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 104..287 e-value: 2.5E-51 score: 176.4
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 294..464 e-value: 4.7E-45 score: 155.5
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 121..464
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 309..460 e-value: 3.1E-32 score: 111.6
None	No IPR available	PANTHER	PTHR13683:SF265	PROTEIN ASPARTIC PROTEASE IN GUARD CELL 2	coord: 59..464
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR13683	ASPARTYL PROTEASES	coord: 59..464
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 125..460 score: 46.532806

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0040637.1	Lag0040637.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity