Lag0040628 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0040628
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Alpha-galactosidase
Location	chr13: 6692412 .. 6695765 (-)
RNA-Seq Expression	Lag0040628
Synteny	Lag0040628

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGCTCCGGAGTAGCAGTGGAGCGGCAATGGCGGTGTTTGTTGTAGTGTTCTCATCGGTTCTGGTGGCGAGAGTTTCGGCCTCTTCCAGAATGACGGAGATCGCCGCCGACGGTGAGTTGTTGAGGCGGAATCTGCTTGCAAATGGCCTCGGAGTTACTCCTCCGATGGGGTGAGTTCTCGGATCTACTACAATCTGATCGGTTTTTTCGTTTGTATGTGTATATTCCTCTGTTTTCTATTCAGATGATGTTGGTTGTTTGAACTGGATTTGTATTTTGTAGGTTAATTTGTGGTTCTGTTGGAATCGATTAGATTTTCGACTTTTCTTCTCTCTCTTTCGAACTTTTGATTATTATCCGGTGGAAATTTTGGACGAACAATGTCCTAGGAGTTTTTCTTTATTTTGCTAATGTTTATATTCGATTGTCAAGGAGAAAAGATTGACGTTATTGGCACACATTTCTCTAGTTATCTTATTCGTCTCTGTGTTTGTTCTTTTCATTATTGTAATAGCTAGATGCGTTCTCTCTCGCTTTTTGGATAAATTATGTTTTATTTTCTTCTTTCTAATTGGAGGAACACTGTTGATGAAACTGTTCTTATTTGAATTAGAACCTTTCTTGTTGTTTGGATTGGACAGATGGAATAGCTGGAATCACTTTGCATGCAATATCAATGAAAAAATGATTAAAGAAACTGGTAAAATTCGCCACTCAATATCTTTTACTTCATCAGTCAAACATTAAATAATGATTAATGACAAAAAGACCTGGATCGACAGCTGATGCTATGGTTTCCACTGGGCTTTCGAAGCTTGGATATGAATATGTTAATATCGGTATGGGCCGATCTCTTCTCTCTTTCTCTAATATTTCAAGATTCAAAAGGTAAGTTATGTGATATTTTGCTTTTCTTCTTAGATGATTGCTGGGCTGAGATAGCTCGTGACGATAAAGTAATTTCTTATCCTCCAGCCTTTTATTCTTTCTTGAGATTGCAATAAAACCGTTTGATTTTATGTCTCTGAGAGTTCTGACTTCTATTTAATTTGATTTCAGGGTTATCTGGTGCCGAAGAAATCGACATTTCCATCTGGCATGAAGGCCCTTGCGGACTATGTTCACTCCAAGGGTCTTAAGCTTGGAATCTATTCAGATGCTGGGTAAAAATAATTTTCAGATGCAGAAATTCACAATCGATATTTTATTTTTTTTTCATTTCATTTGTTGATTTTGAAAAGTTCATATTTATCTTTATGTTTATGAATTAAATTTTTTCCGAACTTTGTTGGGCACAAGCACACTAGGTAGTTTTAATAAATTTAATCTATAATAATAATTATTTATGAATTTGGTAAGTTAATTAGGAGCCAGACTAAAATAGATAAGTTTTCAATTTTATGATTCACATATTTAATTTAAAATATGGGATAATTTCAACTCATTTGAATCTGACCTTTTGAGACATTAAAGGACTTGGGCATGAATGATGAATTGAAAATTGTAAATATTTGGGACAAATATATATAGATATTTTTAAAAAGGACAAATATAGTATGGAATTTCATTTTTAACATTTTCATCTCTTTGCATATTCTATATATGTAAAGTTGATCTACCATATTCAGGTATTAAATTATCATGCAACTCTGCAGGTATTTTACTTGTAGCAAAACCATGCCCGGATCTCTTGGCCATGAGGAACAAGACGCCAAAACCTTTGCTTCATGGGTATGAACAACATGGAATTCCCATTATCAAGTTTTTACAAATTAAAGCAAACTTTCTTCCTCTTTTTCTTCTCTTCCTTTTTGTTCATCTTTTAGTTGGAAGATTGTTGTTAATGCGCACTGTTTTACTTGGCAGGGGATTGACTATTTGAAGTATGATAATTGCAACAATGGTAACATTAAGCCAACTATTAGGTAATGTAATTGCCGCCATAAATCAATCAGTTCACTTCTTTTTCCTGTTTGCCATATGTGGTGGTCTGAAAGTTGAAGCAGCCTGTTAATTATCTCTTCTTTTTCAGATATCCGGTCATGACCCGAGCGCTGATGAAGGCCGGGCGCCCGATCTTCTTCTCTCTTTGTGAATGGTAACTCCTCCTACTTATCCCCAACTCAGGCTAGCCATATGAAACTGTTCCTTCATATCTATTATCATTTATGGCATTCCCTCTCTTGCTTTGTTCTGCAGGGGAGACCTGCACCCTGCTTTGTGGGGCGATAAGTTAGGAAACAGCTGGAGAACTACTAACGACATTAACGATTCGTGGGAGAGGTTGGATTTTCAAGAAAATTTTGGTTAGTTGCTTATATTTGATCATCTGAAACTGAAGGACTAAAGCATTGGAGATTGCATTTGCAGTATGATATCTAGAGCAGACCTCAATGAACCGTATGCTGATCTCGCAAGGCCCGGTGGCTGGAACGGTAGAGTATTCTCCATATCGAACCAAATCATAACTCATAGTGTCTTCTTCATATTGATTAATCATTCTTGCAAACTGTTATCAGATCCTGATATGCTTGAGGTGGGAAATGGAGGGATGACTAAAGATGAATACATTGTCCACTTCAGTCTTTGGGCCATTTCCAAGGTATTGTGCAGCAAAAATTTATATCCTTTCCCTTTCTTTTTGGAGATTGAAAGCCCTGTTTGCTAATGTAGTACTTGTTGGGTGGTGGTGTTGTAGGCTCCCCTTCTTCTTGGTTGTGATTTGAGGAATCTAACAAAAGAAACAAAGGAAATCATTACTAACACAGAAGTCATTGCAGTTAACCAAGGTACAAAATAAATGATTGTTACTCTTCCTTCTGTCAATATGATCGCTTGCTTCTCAATCTCATCGACCTCACTCGATAATTCTCGAAACAGATCCCCTCGGCGTCCAGGCCAAGAAGGTCAGAAGTGAAGGTGATCTAGAGGTAAAGTTCTACAACACTCTGTCACCATTGCCAGAGGCATCATCCTTTTGTTGCAGAGCAAGTAAGAGCAAAACATCCATTTGTTTACTGTTGTTTCCAGGTTTGGGCAGGGCCTCTCTCCGGCTACCGAGTAGCGCTCGTGTTGCTGAACCGAGGGCCGTGGCGTAGTGCGATCACCGCCCAATGGGAGGACATCGGCATCCCTCCAAACAGTAATGTTCAAGCAAGAGATCTTTGGGAGGTAATTTGCTAGTATCATCCCCAAATTTCATGTGTATTATGTTTCAATGACATGTATTTATGATGTCTGTGTGTGAAAATTTTGCAGCACACAACCTTGAAGACTATTTTTGTGGCAAATTTGACAGCTACTTTGGATTCTCATTCCTGCAAACTTTATATTTTGAAGCCAATTTCTTAA

mRNA sequence

ATGGAGCTCCGGAGTAGCAGTGGAGCGGCAATGGCGGTGTTTGTTGTAGTGTTCTCATCGGTTCTGGTGGCGAGAGTTTCGGCCTCTTCCAGAATGACGGAGATCGCCGCCGACGGTGAGTTGTTGAGGCGGAATCTGCTTGCAAATGGCCTCGGAGTTACTCCTCCGATGGGATGGAATAGCTGGAATCACTTTGCATGCAATATCAATGAAAAAATGATTAAAGAAACTGCTGATGCTATGGTTTCCACTGGGCTTTCGAAGCTTGGATATGAATATGTTAATATCGATGATTGCTGGGCTGAGATAGCTCGTGACGATAAAGGTTATCTGGTGCCGAAGAAATCGACATTTCCATCTGGCATGAAGGCCCTTGCGGACTATGTTCACTCCAAGGGTCTTAAGCTTGGAATCTATTCAGATGCTGGGTATTTTACTTGTAGCAAAACCATGCCCGGATCTCTTGGCCATGAGGAACAAGACGCCAAAACCTTTGCTTCATGGGGGATTGACTATTTGAAGTATGATAATTGCAACAATGGTAACATTAAGCCAACTATTAGATATCCGGTCATGACCCGAGCGCTGATGAAGGCCGGGCGCCCGATCTTCTTCTCTCTTTGTGAATGGGGAGACCTGCACCCTGCTTTGTGGGGCGATAAGTTAGGAAACAGCTGGAGAACTACTAACGACATTAACGATTCGTGGGAGAGTATGATATCTAGAGCAGACCTCAATGAACCGTATGCTGATCTCGCAAGGCCCGGTGGCTGGAACGATCCTGATATGCTTGAGGTGGGAAATGGAGGGATGACTAAAGATGAATACATTGTCCACTTCAGTCTTTGGGCCATTTCCAAGGCTCCCCTTCTTCTTGGTTGTGATTTGAGGAATCTAACAAAAGAAACAAAGGAAATCATTACTAACACAGAAGTCATTGCAGTTAACCAAGATCCCCTCGGCGTCCAGGCCAAGAAGGTCAGAAGTGAAGGTGATCTAGAGGTTTGGGCAGGGCCTCTCTCCGGCTACCGAGTAGCGCTCGTGTTGCTGAACCGAGGGCCGTGGCGTAGTGCGATCACCGCCCAATGGGAGGACATCGGCATCCCTCCAAACAGTAATGTTCAAGCAAGAGATCTTTGGGAGCACACAACCTTGAAGACTATTTTTGTGGCAAATTTGACAGCTACTTTGGATTCTCATTCCTGCAAACTTTATATTTTGAAGCCAATTTCTTAA

Coding sequence (CDS)

ATGGAGCTCCGGAGTAGCAGTGGAGCGGCAATGGCGGTGTTTGTTGTAGTGTTCTCATCGGTTCTGGTGGCGAGAGTTTCGGCCTCTTCCAGAATGACGGAGATCGCCGCCGACGGTGAGTTGTTGAGGCGGAATCTGCTTGCAAATGGCCTCGGAGTTACTCCTCCGATGGGATGGAATAGCTGGAATCACTTTGCATGCAATATCAATGAAAAAATGATTAAAGAAACTGCTGATGCTATGGTTTCCACTGGGCTTTCGAAGCTTGGATATGAATATGTTAATATCGATGATTGCTGGGCTGAGATAGCTCGTGACGATAAAGGTTATCTGGTGCCGAAGAAATCGACATTTCCATCTGGCATGAAGGCCCTTGCGGACTATGTTCACTCCAAGGGTCTTAAGCTTGGAATCTATTCAGATGCTGGGTATTTTACTTGTAGCAAAACCATGCCCGGATCTCTTGGCCATGAGGAACAAGACGCCAAAACCTTTGCTTCATGGGGGATTGACTATTTGAAGTATGATAATTGCAACAATGGTAACATTAAGCCAACTATTAGATATCCGGTCATGACCCGAGCGCTGATGAAGGCCGGGCGCCCGATCTTCTTCTCTCTTTGTGAATGGGGAGACCTGCACCCTGCTTTGTGGGGCGATAAGTTAGGAAACAGCTGGAGAACTACTAACGACATTAACGATTCGTGGGAGAGTATGATATCTAGAGCAGACCTCAATGAACCGTATGCTGATCTCGCAAGGCCCGGTGGCTGGAACGATCCTGATATGCTTGAGGTGGGAAATGGAGGGATGACTAAAGATGAATACATTGTCCACTTCAGTCTTTGGGCCATTTCCAAGGCTCCCCTTCTTCTTGGTTGTGATTTGAGGAATCTAACAAAAGAAACAAAGGAAATCATTACTAACACAGAAGTCATTGCAGTTAACCAAGATCCCCTCGGCGTCCAGGCCAAGAAGGTCAGAAGTGAAGGTGATCTAGAGGTTTGGGCAGGGCCTCTCTCCGGCTACCGAGTAGCGCTCGTGTTGCTGAACCGAGGGCCGTGGCGTAGTGCGATCACCGCCCAATGGGAGGACATCGGCATCCCTCCAAACAGTAATGTTCAAGCAAGAGATCTTTGGGAGCACACAACCTTGAAGACTATTTTTGTGGCAAATTTGACAGCTACTTTGGATTCTCATTCCTGCAAACTTTATATTTTGAAGCCAATTTCTTAA

Protein sequence

MELRSSSGAAMAVFVVVFSSVLVARVSASSRMTEIAADGELLRRNLLANGLGVTPPMGWNSWNHFACNINEKMIKETADAMVSTGLSKLGYEYVNIDDCWAEIARDDKGYLVPKKSTFPSGMKALADYVHSKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCNNGNIKPTIRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESMISRADLNEPYADLARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSGYRVALVLLNRGPWRSAITAQWEDIGIPPNSNVQARDLWEHTTLKTIFVANLTATLDSHSCKLYILKPIS

Homology

[Close]

BLAST of Lag0040628 vs. NCBI nr
Match: KAG6573765.1 (Alpha-galactosidase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 813.9 bits (2101), Expect = 6.2e-232
Identity = 387/412 (93.93%), Postives = 400/412 (97.09%), Query Frame = 0


Query: 1   MELRS-SSGAAMAVFVVVFSSVLVARVSASSRMTEIAADGELLRRNLLANGLGVTPPMGW 60
           MELRS   GAAMAVFV+VFSSV+V  V A+SR+ EI+A GELLRRNLLANGLGVTPPMGW
Sbjct: 1   MELRSYCGGAAMAVFVLVFSSVMVMSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGW 60

Query: 61  NSWNHFACNINEKMIKETADAMVSTGLSKLGYEYVNIDDCWAEIARDDKGYLVPKKSTFP 120
           NSWNHFACNINE MIK+TADA+VSTGLSKLGYEYVNIDDCWAEIARDDKG LVPKKSTFP
Sbjct: 61  NSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFP 120

Query: 121 SGMKALADYVHSKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180
           SGMKALADYVH+KGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN
Sbjct: 121 SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180

Query: 181 NGNIKPTIRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240
           NGNIKPT+RYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM
Sbjct: 181 NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240

Query: 241 ISRADLNEPYADLARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNL 300
           ISRADLNEPYAD ARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNL
Sbjct: 241 ISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNL 300

Query: 301 TKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSGYRVALVLLNRGPWRSAI 360
           TKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLS YRVALVLLNRGPWRS+I
Sbjct: 301 TKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSI 360

Query: 361 TAQWEDIGIPPNSNVQARDLWEHTTLKTIFVANLTATLDSHSCKLYILKPIS 412
           TAQW+DIGIPPNSNVQARDLWEHTTLKTIFVANLTAT+DSH+CKLYILKPIS
Sbjct: 361 TAQWDDIGIPPNSNVQARDLWEHTTLKTIFVANLTATVDSHACKLYILKPIS 412

[Close]

BLAST of Lag0040628 vs. NCBI nr
Match: KAG7012838.1 (Alpha-galactosidase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 813.5 bits (2100), Expect = 8.1e-232
Identity = 387/412 (93.93%), Postives = 399/412 (96.84%), Query Frame = 0


Query: 1   MELRS-SSGAAMAVFVVVFSSVLVARVSASSRMTEIAADGELLRRNLLANGLGVTPPMGW 60
           MELRS   GAAMAVFV VFSSV+V  V A+SR+ EI+A GELLRRNLLANGLGVTPPMGW
Sbjct: 1   MELRSYCGGAAMAVFVFVFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGW 60

Query: 61  NSWNHFACNINEKMIKETADAMVSTGLSKLGYEYVNIDDCWAEIARDDKGYLVPKKSTFP 120
           NSWNHFACNINE MIK+TADA+VSTGLSKLGYEYVNIDDCWAEIARDDKG LVPKKSTFP
Sbjct: 61  NSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFP 120

Query: 121 SGMKALADYVHSKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180
           SGMKALADYVH+KGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN
Sbjct: 121 SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180

Query: 181 NGNIKPTIRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240
           NGNIKPT+RYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM
Sbjct: 181 NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240

Query: 241 ISRADLNEPYADLARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNL 300
           ISRADLNEPYAD ARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNL
Sbjct: 241 ISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNL 300

Query: 301 TKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSGYRVALVLLNRGPWRSAI 360
           TKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLS YRVALVLLNRGPWRS+I
Sbjct: 301 TKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSI 360

Query: 361 TAQWEDIGIPPNSNVQARDLWEHTTLKTIFVANLTATLDSHSCKLYILKPIS 412
           TAQW+DIGIPPNSNVQARDLWEHTTLKTIFVANLTAT+DSH+CKLYILKPIS
Sbjct: 361 TAQWDDIGIPPNSNVQARDLWEHTTLKTIFVANLTATVDSHACKLYILKPIS 412

[Close]

BLAST of Lag0040628 vs. NCBI nr
Match: XP_022966950.1 (alpha-galactosidase 1-like [Cucurbita maxima])

HSP 1 Score: 812.0 bits (2096), Expect = 2.4e-231
Identity = 385/412 (93.45%), Postives = 400/412 (97.09%), Query Frame = 0


Query: 1   MELRS-SSGAAMAVFVVVFSSVLVARVSASSRMTEIAADGELLRRNLLANGLGVTPPMGW 60
           MELRS   GAAMAVFV+VFSSV+V  V A+SR+ EI+A GELLRRNLLANGLGVTPPMGW
Sbjct: 1   MELRSYCGGAAMAVFVLVFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGW 60

Query: 61  NSWNHFACNINEKMIKETADAMVSTGLSKLGYEYVNIDDCWAEIARDDKGYLVPKKSTFP 120
           NSWNHFACNINE MIK+TADA+VSTGLSKLGYEYVNIDDCWAEIARDDKG LVPKKSTFP
Sbjct: 61  NSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFP 120

Query: 121 SGMKALADYVHSKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180
           SGMKALADYVH+KGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN
Sbjct: 121 SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180

Query: 181 NGNIKPTIRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240
           NGNIKPT+RYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM
Sbjct: 181 NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240

Query: 241 ISRADLNEPYADLARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNL 300
           ISRADLNEPYAD ARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNL
Sbjct: 241 ISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNL 300

Query: 301 TKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSGYRVALVLLNRGPWRSAI 360
           TKETKEIITNTEVIAVNQDPLG+QAKKVRSEGDLEVWAGPLS YRVALVLLNRGPWRS+I
Sbjct: 301 TKETKEIITNTEVIAVNQDPLGIQAKKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSI 360

Query: 361 TAQWEDIGIPPNSNVQARDLWEHTTLKTIFVANLTATLDSHSCKLYILKPIS 412
           TAQW+DIGIPPNSNVQARDLWEHTTLKTIFVANLTAT++SH+CKLYILKPIS
Sbjct: 361 TAQWDDIGIPPNSNVQARDLWEHTTLKTIFVANLTATVNSHACKLYILKPIS 412

[Close]

BLAST of Lag0040628 vs. NCBI nr
Match: XP_023541761.1 (alpha-galactosidase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 810.4 bits (2092), Expect = 6.9e-231
Identity = 384/412 (93.20%), Postives = 399/412 (96.84%), Query Frame = 0


Query: 1   MELRS-SSGAAMAVFVVVFSSVLVARVSASSRMTEIAADGELLRRNLLANGLGVTPPMGW 60
           MELRS   GAAMAVFV+VFSSV+V  V A+SR+ EI+A GELLRRNLLANGLGVTPPMGW
Sbjct: 1   MELRSYCGGAAMAVFVLVFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGW 60

Query: 61  NSWNHFACNINEKMIKETADAMVSTGLSKLGYEYVNIDDCWAEIARDDKGYLVPKKSTFP 120
           NSWNHFACNINE MIK+TADA+VSTGLSKLGYEYVNIDDCWAEIARDDKG LVPKKSTFP
Sbjct: 61  NSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFP 120

Query: 121 SGMKALADYVHSKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180
           SGMKALADYVH+KGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN
Sbjct: 121 SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180

Query: 181 NGNIKPTIRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240
           NGNIKPT+RYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM
Sbjct: 181 NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240

Query: 241 ISRADLNEPYADLARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNL 300
           ISRADLNEPYAD ARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNL
Sbjct: 241 ISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNL 300

Query: 301 TKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSGYRVALVLLNRGPWRSAI 360
           TKETKEIITNTEVIAVNQDPLG+QAKKVRSEGDLEVWAGPLS YRVALVLLNRGPWRS+I
Sbjct: 301 TKETKEIITNTEVIAVNQDPLGIQAKKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSI 360

Query: 361 TAQWEDIGIPPNSNVQARDLWEHTTLKTIFVANLTATLDSHSCKLYILKPIS 412
           TAQW+DIGIPPNSNV+ARDLWEHTTLKTIFVANLTAT+DSH+CKLYIL PIS
Sbjct: 361 TAQWDDIGIPPNSNVRARDLWEHTTLKTIFVANLTATVDSHACKLYILNPIS 412

[Close]

BLAST of Lag0040628 vs. NCBI nr
Match: XP_022945946.1 (alpha-galactosidase 1-like [Cucurbita moschata])

HSP 1 Score: 810.4 bits (2092), Expect = 6.9e-231
Identity = 385/412 (93.45%), Postives = 398/412 (96.60%), Query Frame = 0


Query: 1   MELRS-SSGAAMAVFVVVFSSVLVARVSASSRMTEIAADGELLRRNLLANGLGVTPPMGW 60
           MELRS   GAAM VFV VFSSV+V  V A+SR+ EI+A GELLRRNLLANGLGVTPPMGW
Sbjct: 1   MELRSYCGGAAMGVFVFVFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGW 60

Query: 61  NSWNHFACNINEKMIKETADAMVSTGLSKLGYEYVNIDDCWAEIARDDKGYLVPKKSTFP 120
           NSWNHFACNINE MIK+TADA+VSTGLSKLGYEYVNIDDCWAEIARDDKG LVPKKSTFP
Sbjct: 61  NSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFP 120

Query: 121 SGMKALADYVHSKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180
           SGMKALADYVH+KGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN
Sbjct: 121 SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180

Query: 181 NGNIKPTIRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240
           NGNIKPT+RYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM
Sbjct: 181 NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240

Query: 241 ISRADLNEPYADLARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNL 300
           ISRADLNEPYAD ARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNL
Sbjct: 241 ISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNL 300

Query: 301 TKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSGYRVALVLLNRGPWRSAI 360
           TKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLS YRVALVLLNRGPWRS+I
Sbjct: 301 TKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSI 360

Query: 361 TAQWEDIGIPPNSNVQARDLWEHTTLKTIFVANLTATLDSHSCKLYILKPIS 412
           TAQW+DIGIPPNSNV+ARDLWEHTTLKTIFVANLTAT+DSH+CKLYILKPIS
Sbjct: 361 TAQWDDIGIPPNSNVRARDLWEHTTLKTIFVANLTATVDSHACKLYILKPIS 412

[Close]

BLAST of Lag0040628 vs. ExPASy Swiss-Prot
Match: Q9FT97 (Alpha-galactosidase 1 OS=Arabidopsis thaliana OX=3702 GN=AGAL1 PE=2 SV=1)

HSP 1 Score: 686.4 bits (1770), Expect = 2.0e-196
Identity = 315/401 (78.55%), Postives = 356/401 (88.78%), Query Frame = 0


Query: 11  MAVFVVVFSSVLVARVSASSRMTEIAADGELLRRNLLANGLGVTPPMGWNSWNHFACNIN 70
           M + +++ SS+++  V +S  +     D E+LRR+LL NGLGVTPPMGWNSWNHF+CNI+
Sbjct: 10  MPILMILISSMVMTMVESSRSVNNGHDDSEILRRHLLTNGLGVTPPMGWNSWNHFSCNID 69

Query: 71  EKMIKETADAMVSTGLSKLGYEYVNIDDCWAEIARDDKGYLVPKKSTFPSGMKALADYVH 130
           EKMIKETADA+V+TGLSKLGY YVNIDDCWAEI+RD KG LVPKKSTFPSG+KA+ADYVH
Sbjct: 70  EKMIKETADALVTTGLSKLGYNYVNIDDCWAEISRDSKGSLVPKKSTFPSGIKAVADYVH 129

Query: 131 SKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCNNGNIKPTIRYP 190
           SKGLKLGIYSDAGYFTCSKTMPGSLG+EE DAKTFA WGIDYLKYDNCN+   KPT+RYP
Sbjct: 130 SKGLKLGIYSDAGYFTCSKTMPGSLGYEEHDAKTFAEWGIDYLKYDNCNSDGSKPTVRYP 189

Query: 191 VMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESMISRADLNEPYA 250
           VMTRALMK+GRPIF SLCEWGD+HPALWG  +GNSWRTTNDI D+W SMIS AD+NE YA
Sbjct: 190 VMTRALMKSGRPIFHSLCEWGDMHPALWGSPVGNSWRTTNDIKDTWLSMISIADMNEVYA 249

Query: 251 DLARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITNT 310
           + ARPGGWNDPDMLEVGNGGMTKDEYIVHFS+WAISKAPLLLGCD+RN+TKET EI+ N 
Sbjct: 250 EHARPGGWNDPDMLEVGNGGMTKDEYIVHFSIWAISKAPLLLGCDIRNMTKETMEIVANK 309

Query: 311 EVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSGYRVALVLLNRGPWRSAITAQWEDIGIPP 370
           EVIA+NQDP GVQAKKVR EGDLEVWAGPLSGYRVAL+LLNRGP R++ITA WEDI IP 
Sbjct: 310 EVIAINQDPHGVQAKKVRMEGDLEVWAGPLSGYRVALLLLNRGPSRTSITALWEDIEIPA 369

Query: 371 NSNVQARDLWEHTTLKTIFVANLTATLDSHSCKLYILKPIS 412
           NS V+ARDLWEH TLK  FV NLTAT+DSH+CKLY+LKP++
Sbjct: 370 NSIVEARDLWEHQTLKQKFVGNLTATVDSHACKLYVLKPVA 410

[Close]

BLAST of Lag0040628 vs. ExPASy Swiss-Prot
Match: Q42656 (Alpha-galactosidase OS=Coffea arabica OX=13443 PE=1 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 5.0e-160
Identity = 251/367 (68.39%), Postives = 304/367 (82.83%), Query Frame = 0


Query: 43  RRNLLANGLGVTPPMGWNSWNHFACNINEKMIKETADAMVSTGLSKLGYEYVNIDDCWAE 102
           RR+LLANGLG+TPPMGWNSWNHF CN++EK+I+ETADAMVS GL+ LGY+Y+N+DDCWAE
Sbjct: 12  RRSLLANGLGLTPPMGWNSWNHFRCNLDEKLIRETADAMVSKGLAALGYKYINLDDCWAE 71

Query: 103 IARDDKGYLVPKKSTFPSGMKALADYVHSKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDA 162
           + RD +G LVPK STFPSG+KALADYVHSKGLKLGIYSDAG  TCSKTMPGSLGHEEQDA
Sbjct: 72  LNRDSQGNLVPKGSTFPSGIKALADYVHSKGLKLGIYSDAGTQTCSKTMPGSLGHEEQDA 131

Query: 163 KTFASWGIDYLKYDNCNNGNIKPTIRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKL 222
           KTFASWG+DYLKYDNCNN NI P  RYP+M++AL+ +GR IFFSLCEWG+  PA W  ++
Sbjct: 132 KTFASWGVDYLKYDNCNNNNISPKERYPIMSKALLNSGRSIFFSLCEWGEEDPATWAKEV 191

Query: 223 GNSWRTTNDINDSWESMISRADLNEPYADLARPGGWNDPDMLEVGNGGMTKDEYIVHFSL 282
           GNSWRTT DI+DSW SM SRAD+N+ +A  A PGGWNDPDMLEVGNGGMT  EY  HFS+
Sbjct: 192 GNSWRTTGDIDDSWSSMTSRADMNDKWASYAGPGGWNDPDMLEVGNGGMTTTEYRSHFSI 251

Query: 283 WAISKAPLLLGCDLRNLTKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSG 342
           WA++KAPLL+GCD+R++   T ++++N EVIAVNQD LGVQ  KV++ GDLEVWAGPLSG
Sbjct: 252 WALAKAPLLIGCDIRSMDGATFQLLSNAEVIAVNQDKLGVQGNKVKTYGDLEVWAGPLSG 311

Query: 343 YRVALVLLNRGPWRSAITAQWEDIGIPPNSNVQARDLWEHTTLKTIFVANLTATLDSHSC 402
            RVA+ L NRG   + ITA W D+G+P  + V ARDLW H+T K++    ++A +D+H  
Sbjct: 312 KRVAVALWNRGSSTATITAYWSDVGLPSTAVVNARDLWAHSTEKSV-KGQISAAVDAHDS 371

Query: 403 KLYILKP 410
           K+Y+L P
Sbjct: 372 KMYVLTP 377

[Close]

BLAST of Lag0040628 vs. ExPASy Swiss-Prot
Match: P14749 (Alpha-galactosidase OS=Cyamopsis tetragonoloba OX=3832 PE=1 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 1.9e-151
Identity = 245/404 (60.64%), Postives = 303/404 (75.00%), Query Frame = 0


Query: 8   GAAMAVFVVVFSSVLVARVSASSRMTEIAADGELLRRNLLANGLGVTPPMGWNSWNHFAC 67
           G  + V +++       R+      T   A+   +RR L  NGLG TPPMGWNSWNHF C
Sbjct: 9   GGMIIVVLLMIIGSEGGRLLEKKNRTSAEAEHYNVRRYLAENGLGQTPPMGWNSWNHFGC 68

Query: 68  NINEKMIKETADAMVSTGLSKLGYEYVNIDDCWAEIARDDKGYLVPKKSTFPSGMKALAD 127
           +INE +++ETADAMVSTGL+ LGY+Y+N+DDCWAE+ RD +G +VP  + FPSG+KALAD
Sbjct: 69  DINENVVRETADAMVSTGLAALGYQYINLDDCWAELNRDSEGNMVPNAAAFPSGIKALAD 128

Query: 128 YVHSKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCNNGNIKPTI 187
           YVHSKGLKLG+YSDAG  TCSK MPGSLGHEEQDAKTFASWG+DYLKYDNC N  I    
Sbjct: 129 YVHSKGLKLGVYSDAGNQTCSKRMPGSLGHEEQDAKTFASWGVDYLKYDNCENLGISVKE 188

Query: 188 RYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESMISRADLNE 247
           RYP M +AL+ +GRPIFFS+CEWG   P +W   +GNSWRTT DI D+W SM S AD N+
Sbjct: 189 RYPPMGKALLSSGRPIFFSMCEWGWEDPQIWAKSIGNSWRTTGDIEDNWNSMTSIADSND 248

Query: 248 PYADLARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEII 307
            +A  A PGGWNDPDMLEVGNGGMT +EY  HFS+WA++KAPLL+GCD+R +   T E+I
Sbjct: 249 KWASYAGPGGWNDPDMLEVGNGGMTTEEYRSHFSIWALAKAPLLVGCDIRAMDDTTHELI 308

Query: 308 TNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSGYRVALVLLNRGPWRSAITAQWEDIG 367
           +N EVIAVNQD LGVQ KKV+S  DLEVWAGPLS  +VA++L NR   R+ +TA W DIG
Sbjct: 309 SNAEVIAVNQDKLGVQGKKVKSTNDLEVWAGPLSDNKVAVILWNRSSSRATVTASWSDIG 368

Query: 368 IPPNSNVQARDLWEHTTLKTIFVANLTATLDSHSCKLYILKPIS 412
           +   + V ARDLWEH+T +++    ++A +DSH+CK+Y+L P S
Sbjct: 369 LQQGTTVDARDLWEHST-QSLVSGEISAEIDSHACKMYVLTPRS 411

[Close]

BLAST of Lag0040628 vs. ExPASy Swiss-Prot
Match: Q8RX86 (Alpha-galactosidase 2 OS=Arabidopsis thaliana OX=3702 GN=AGAL2 PE=1 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 5.6e-151
Identity = 246/376 (65.43%), Postives = 298/376 (79.26%), Query Frame = 0


Query: 32  MTEIAADGELLRRNLLANGLGVTPPMGWNSWNHFACNINEKMIKETADAMVSTGLSKLGY 91
           +T+I ADG    R L+ NGL ++P MGWNSWNHF CNINE +IK+TADAMVS+GLS +GY
Sbjct: 19  LTQI-ADG-FQSRMLMNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSAIGY 78

Query: 92  EYVNIDDCWAEIARDDKGYLVPKKSTFPSGMKALADYVHSKGLKLGIYSDAGYFTCSKTM 151
           +Y+NIDDCW E+ RD +G LV K STFPSG+KAL+DYVHSKGLKLGIYSDAG  TCS+TM
Sbjct: 79  KYINIDDCWGELKRDSQGSLVAKASTFPSGIKALSDYVHSKGLKLGIYSDAGTLTCSQTM 138

Query: 152 PGSLGHEEQDAKTFASWGIDYLKYDNCNNGNIKPTIRYPVMTRALMKAGRPIFFSLCEWG 211
           PGSLGHEEQDAKTFASWGIDYLKYDNC N    P  RYP M++AL+ +GR IFFSLCEWG
Sbjct: 139 PGSLGHEEQDAKTFASWGIDYLKYDNCENTGTSPRERYPKMSKALLNSGRSIFFSLCEWG 198

Query: 212 DLHPALWGDKLGNSWRTTNDINDSWESMISRADLNEPYADLARPGGWNDPDMLEVGNGGM 271
              PA W   +GNSWRTT DI D+W+SM   AD N+ +A  ARPG WNDPDMLEVGNGGM
Sbjct: 199 QEDPATWAGDIGNSWRTTGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGGM 258

Query: 272 TKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITNTEVIAVNQDPLGVQAKKVRSEG 331
           TK+EY+ HFS+WA++KAPLL+GCDLR++ K T E+++N EVIAVNQD LG+Q KKV+ EG
Sbjct: 259 TKEEYMSHFSIWALAKAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVKKEG 318

Query: 332 DLEVWAGPLSGYRVALVLLNRGPWRSAITAQWEDIGIPPNSNVQARDLWEHTTLKTIFVA 391
           DLEVWAGPLS  RVA++L NRG   + ITA+W +IG+  +  V ARDLWEH+T   +   
Sbjct: 319 DLEVWAGPLSKKRVAVILWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYSCV-KK 378

Query: 392 NLTATLDSHSCKLYIL 408
            L+A ++ H+CK+Y L
Sbjct: 379 QLSALVEPHACKMYTL 391

[Close]

BLAST of Lag0040628 vs. ExPASy Swiss-Prot
Match: Q9FXT4 (Alpha-galactosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0493600 PE=1 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 9.6e-143
Identity = 229/367 (62.40%), Postives = 284/367 (77.38%), Query Frame = 0


Query: 43  RRNLLANGLGVTPPMGWNSWNHFACNINEKMIKETADAMVSTGLSKLGYEYVNIDDCWAE 102
           RR    NGLG TP MGWNSWNHF C INE++I+ETADA+V+TGL+KLGY+YVNIDDCWAE
Sbjct: 52  RRRAFENGLGRTPQMGWNSWNHFYCGINEQIIRETADALVNTGLAKLGYQYVNIDDCWAE 111

Query: 103 IARDDKGYLVPKKSTFPSGMKALADYVHSKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDA 162
            +RD +G  VP + TFPSG+KALADYVH+KGLKLGIYSDAG  TCS  MPGSL HEEQD 
Sbjct: 112 YSRDSQGNFVPNRQTFPSGIKALADYVHAKGLKLGIYSDAGSQTCSNKMPGSLDHEEQDV 171

Query: 163 KTFASWGIDYLKYDNCNNGNIKPTIRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKL 222
           KTFASWG+DYLKYDNCN+       RY  M+ A+   G+ IFFSLCEWG  +PA W  ++
Sbjct: 172 KTFASWGVDYLKYDNCNDAGRSVMERYTRMSNAMKTYGKNIFFSLCEWGKENPATWAGRM 231

Query: 223 GNSWRTTNDINDSWESMISRADLNEPYADLARPGGWNDPDMLEVGNGGMTKDEYIVHFSL 282
           GNSWRTT DI D+W SM SRAD N+ +A  A PGGWNDPDMLEVGNGGM++ EY  HFS+
Sbjct: 232 GNSWRTTGDIADNWGSMTSRADENDQWAAYAGPGGWNDPDMLEVGNGGMSEAEYRSHFSI 291

Query: 283 WAISKAPLLLGCDLRNLTKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSG 342
           WA++KAPLL+GCD+R+++++TK I++N+EVIAVNQD LGVQ KKV+S+  LEVWAGPLS 
Sbjct: 292 WALAKAPLLIGCDVRSMSQQTKNILSNSEVIAVNQDSLGVQGKKVQSDNGLEVWAGPLSN 351

Query: 343 YRVALVLLNRGPWRSAITAQWEDIGIPPNSNVQARDLWEHTTLKTIFVANLTATLDSHSC 402
            R A+VL NR  +++ ITA W +IG+  +  V ARDLW H++        ++A++  H C
Sbjct: 352 NRKAVVLWNRQSYQATITAHWSNIGLAGSVAVTARDLWAHSSFAA--QGQISASVAPHDC 411

Query: 403 KLYILKP 410
           K+Y+L P
Sbjct: 412 KMYVLTP 416

[Close]

BLAST of Lag0040628 vs. ExPASy TrEMBL
Match: A0A6J1HT03 (Alpha-galactosidase OS=Cucurbita maxima OX=3661 GN=LOC111466509 PE=3 SV=1)

HSP 1 Score: 812.0 bits (2096), Expect = 1.1e-231
Identity = 385/412 (93.45%), Postives = 400/412 (97.09%), Query Frame = 0


Query: 1   MELRS-SSGAAMAVFVVVFSSVLVARVSASSRMTEIAADGELLRRNLLANGLGVTPPMGW 60
           MELRS   GAAMAVFV+VFSSV+V  V A+SR+ EI+A GELLRRNLLANGLGVTPPMGW
Sbjct: 1   MELRSYCGGAAMAVFVLVFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGW 60

Query: 61  NSWNHFACNINEKMIKETADAMVSTGLSKLGYEYVNIDDCWAEIARDDKGYLVPKKSTFP 120
           NSWNHFACNINE MIK+TADA+VSTGLSKLGYEYVNIDDCWAEIARDDKG LVPKKSTFP
Sbjct: 61  NSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFP 120

Query: 121 SGMKALADYVHSKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180
           SGMKALADYVH+KGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN
Sbjct: 121 SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180

Query: 181 NGNIKPTIRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240
           NGNIKPT+RYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM
Sbjct: 181 NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240

Query: 241 ISRADLNEPYADLARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNL 300
           ISRADLNEPYAD ARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNL
Sbjct: 241 ISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNL 300

Query: 301 TKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSGYRVALVLLNRGPWRSAI 360
           TKETKEIITNTEVIAVNQDPLG+QAKKVRSEGDLEVWAGPLS YRVALVLLNRGPWRS+I
Sbjct: 301 TKETKEIITNTEVIAVNQDPLGIQAKKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSI 360

Query: 361 TAQWEDIGIPPNSNVQARDLWEHTTLKTIFVANLTATLDSHSCKLYILKPIS 412
           TAQW+DIGIPPNSNVQARDLWEHTTLKTIFVANLTAT++SH+CKLYILKPIS
Sbjct: 361 TAQWDDIGIPPNSNVQARDLWEHTTLKTIFVANLTATVNSHACKLYILKPIS 412

[Close]

BLAST of Lag0040628 vs. ExPASy TrEMBL
Match: A0A6J1G2A9 (Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111450037 PE=3 SV=1)

HSP 1 Score: 810.4 bits (2092), Expect = 3.3e-231
Identity = 385/412 (93.45%), Postives = 398/412 (96.60%), Query Frame = 0


Query: 1   MELRS-SSGAAMAVFVVVFSSVLVARVSASSRMTEIAADGELLRRNLLANGLGVTPPMGW 60
           MELRS   GAAM VFV VFSSV+V  V A+SR+ EI+A GELLRRNLLANGLGVTPPMGW
Sbjct: 1   MELRSYCGGAAMGVFVFVFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGW 60

Query: 61  NSWNHFACNINEKMIKETADAMVSTGLSKLGYEYVNIDDCWAEIARDDKGYLVPKKSTFP 120
           NSWNHFACNINE MIK+TADA+VSTGLSKLGYEYVNIDDCWAEIARDDKG LVPKKSTFP
Sbjct: 61  NSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFP 120

Query: 121 SGMKALADYVHSKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180
           SGMKALADYVH+KGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN
Sbjct: 121 SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180

Query: 181 NGNIKPTIRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240
           NGNIKPT+RYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM
Sbjct: 181 NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240

Query: 241 ISRADLNEPYADLARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNL 300
           ISRADLNEPYAD ARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNL
Sbjct: 241 ISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNL 300

Query: 301 TKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSGYRVALVLLNRGPWRSAI 360
           TKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLS YRVALVLLNRGPWRS+I
Sbjct: 301 TKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSI 360

Query: 361 TAQWEDIGIPPNSNVQARDLWEHTTLKTIFVANLTATLDSHSCKLYILKPIS 412
           TAQW+DIGIPPNSNV+ARDLWEHTTLKTIFVANLTAT+DSH+CKLYILKPIS
Sbjct: 361 TAQWDDIGIPPNSNVRARDLWEHTTLKTIFVANLTATVDSHACKLYILKPIS 412

[Close]

BLAST of Lag0040628 vs. ExPASy TrEMBL
Match: A0A6J1CC22 (Alpha-galactosidase OS=Momordica charantia OX=3673 GN=LOC111010280 PE=3 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 1.9e-226
Identity = 378/415 (91.08%), Postives = 396/415 (95.42%), Query Frame = 0


Query: 1   MELRS-SSGAAMAVFV---VVFSSVLVARVSASSRMTEIAADGELLRRNLLANGLGVTPP 60
           ME RS   GAAMA+ V   VV+SSV+VA VSA+SRMTEI AD ELLRRNLLANGLGVTPP
Sbjct: 1   MECRSYCGGAAMAMAVLVLVVYSSVMVASVSATSRMTEITADDELLRRNLLANGLGVTPP 60

Query: 61  MGWNSWNHFACNINEKMIKETADAMVSTGLSKLGYEYVNIDDCWAEIARDDKGYLVPKKS 120
           MGWNSWNHFACNINEKMIKETADA++STGLSKLGY+YVNIDDCWAEIARDDKG LVPKKS
Sbjct: 61  MGWNSWNHFACNINEKMIKETADALISTGLSKLGYKYVNIDDCWAEIARDDKGNLVPKKS 120

Query: 121 TFPSGMKALADYVHSKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYD 180
           TFPSGMKALADYVH+KGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYD
Sbjct: 121 TFPSGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYD 180

Query: 181 NCNNGNIKPTIRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSW 240
           NCNNGNIKPT+RYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSW
Sbjct: 181 NCNNGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSW 240

Query: 241 ESMISRADLNEPYADLARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDL 300
           ESMISRADLNE YADLARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWA+SKAPLLLGCDL
Sbjct: 241 ESMISRADLNEVYADLARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWALSKAPLLLGCDL 300

Query: 301 RNLTKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSGYRVALVLLNRGPWR 360
           RNLTKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLS YRVA+VLLNRGPWR
Sbjct: 301 RNLTKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSSYRVAVVLLNRGPWR 360

Query: 361 SAITAQWEDIGIPPNSNVQARDLWEHTTLKTIFVANLTATLDSHSCKLYILKPIS 412
           S +TAQW+DIGIPPNS VQARDLWEH TLK +FV NL+AT+DSH+CK+YILKPIS
Sbjct: 361 SVLTAQWDDIGIPPNSIVQARDLWEHNTLKNVFVGNLSATVDSHACKMYILKPIS 415

[Close]

BLAST of Lag0040628 vs. ExPASy TrEMBL
Match: Q2MK92 (Alpha-galactosidase OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 789.3 bits (2037), Expect = 7.9e-225
Identity = 375/413 (90.80%), Postives = 395/413 (95.64%), Query Frame = 0


Query: 1   MELRSSSGA-AMAVFVVVFSSVLV-ARVSASSRMTEIAADGELLRRNLLANGLGVTPPMG 60
           ME RS   A A+ VFV+ FS V++   VSA+SRMTEIA+DG+LLRRNLLANGLGVTPPMG
Sbjct: 1   MECRSYCKAPAVVVFVLAFSLVMLETTVSATSRMTEIASDGDLLRRNLLANGLGVTPPMG 60

Query: 61  WNSWNHFACNINEKMIKETADAMVSTGLSKLGYEYVNIDDCWAEIARDDKGYLVPKKSTF 120
           WNSWNHFACNINEKMI+ETADA+VSTGLSKLGYEYVNIDDCWAEIARDDKG LVPK STF
Sbjct: 61  WNSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNSTF 120

Query: 121 PSGMKALADYVHSKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNC 180
           PSGMKALADYVH+KGLK+GIYSDAGYFTCSKTMPGSLGHEEQDAKTFA+WGIDYLKYDNC
Sbjct: 121 PSGMKALADYVHAKGLKIGIYSDAGYFTCSKTMPGSLGHEEQDAKTFAAWGIDYLKYDNC 180

Query: 181 NNGNIKPTIRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWES 240
           NNGNIKPTIRYPVMTRALMKAGRPIF SLCEWGDLHPALWGDKLGNSWRTTNDINDSWES
Sbjct: 181 NNGNIKPTIRYPVMTRALMKAGRPIFLSLCEWGDLHPALWGDKLGNSWRTTNDINDSWES 240

Query: 241 MISRADLNEPYADLARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRN 300
           MISRADLNE YAD ARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRN
Sbjct: 241 MISRADLNEIYADYARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRN 300

Query: 301 LTKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSGYRVALVLLNRGPWRSA 360
           LTKETK I+TNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSGYRVA+VLLNRGPWR+A
Sbjct: 301 LTKETKAIVTNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSGYRVAVVLLNRGPWRNA 360

Query: 361 ITAQWEDIGIPPNSNVQARDLWEHTTLKTIFVANLTATLDSHSCKLYILKPIS 412
           I+AQW+DIGIPPNSNV+ARDLWEHTTLKT FVANLTAT+DSH+CKLYILKPIS
Sbjct: 361 ISAQWDDIGIPPNSNVEARDLWEHTTLKTTFVANLTATVDSHACKLYILKPIS 413

[Close]

BLAST of Lag0040628 vs. ExPASy TrEMBL
Match: A0A1S3BDT4 (Alpha-galactosidase OS=Cucumis melo OX=3656 GN=LOC103488793 PE=3 SV=1)

HSP 1 Score: 789.3 bits (2037), Expect = 7.9e-225
Identity = 378/414 (91.30%), Postives = 397/414 (95.89%), Query Frame = 0


Query: 1   MELRS-SSGAAMAVFVVVFSSVLV-ARVSASSRMTE-IAADGELLRRNLLANGLGVTPPM 60
           ME RS  +GAA+AVFV+ FS V++ + VSA+SR TE IA DG+LLRRNLLANGLGVTPPM
Sbjct: 1   MECRSYCTGAAVAVFVLAFSLVMLESTVSATSRRTEIIATDGDLLRRNLLANGLGVTPPM 60

Query: 61  GWNSWNHFACNINEKMIKETADAMVSTGLSKLGYEYVNIDDCWAEIARDDKGYLVPKKST 120
           GWNSWNHFACNINEKMI+ETADA+VSTGLSKLGYEYVNIDDCWAEIARDDKG LVPK ST
Sbjct: 61  GWNSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNST 120

Query: 121 FPSGMKALADYVHSKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDN 180
           FPSGMKALADYVH+KGLK+GIYSDAGYFTCSKTMPGSLGHEEQDAKTFA+WGIDYLKYDN
Sbjct: 121 FPSGMKALADYVHAKGLKIGIYSDAGYFTCSKTMPGSLGHEEQDAKTFAAWGIDYLKYDN 180

Query: 181 CNNGNIKPTIRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWE 240
           CNNGNIKPTIRYPVMTRALMKAGRPIF SLCEWGDLHPALWGDKLGNSWRTTNDINDSWE
Sbjct: 181 CNNGNIKPTIRYPVMTRALMKAGRPIFLSLCEWGDLHPALWGDKLGNSWRTTNDINDSWE 240

Query: 241 SMISRADLNEPYADLARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLR 300
           SMISRADLNE YAD ARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLR
Sbjct: 241 SMISRADLNEIYADYARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLR 300

Query: 301 NLTKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSGYRVALVLLNRGPWRS 360
           NLTKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSGYRVA+VLLNRGPWR+
Sbjct: 301 NLTKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSGYRVAVVLLNRGPWRN 360

Query: 361 AITAQWEDIGIPPNSNVQARDLWEHTTLKTIFVANLTATLDSHSCKLYILKPIS 412
           AITAQW+DIGIPPNSNV+ARDLWE TTLKT FVANLTAT+DSH+CKLYILKPIS
Sbjct: 361 AITAQWDDIGIPPNSNVEARDLWERTTLKTTFVANLTATVDSHACKLYILKPIS 414

[Close]

BLAST of Lag0040628 vs. TAIR 10
Match: AT5G08380.1 (alpha-galactosidase 1 )

HSP 1 Score: 686.4 bits (1770), Expect = 1.4e-197
Identity = 315/401 (78.55%), Postives = 356/401 (88.78%), Query Frame = 0


Query: 11  MAVFVVVFSSVLVARVSASSRMTEIAADGELLRRNLLANGLGVTPPMGWNSWNHFACNIN 70
           M + +++ SS+++  V +S  +     D E+LRR+LL NGLGVTPPMGWNSWNHF+CNI+
Sbjct: 10  MPILMILISSMVMTMVESSRSVNNGHDDSEILRRHLLTNGLGVTPPMGWNSWNHFSCNID 69

Query: 71  EKMIKETADAMVSTGLSKLGYEYVNIDDCWAEIARDDKGYLVPKKSTFPSGMKALADYVH 130
           EKMIKETADA+V+TGLSKLGY YVNIDDCWAEI+RD KG LVPKKSTFPSG+KA+ADYVH
Sbjct: 70  EKMIKETADALVTTGLSKLGYNYVNIDDCWAEISRDSKGSLVPKKSTFPSGIKAVADYVH 129

Query: 131 SKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCNNGNIKPTIRYP 190
           SKGLKLGIYSDAGYFTCSKTMPGSLG+EE DAKTFA WGIDYLKYDNCN+   KPT+RYP
Sbjct: 130 SKGLKLGIYSDAGYFTCSKTMPGSLGYEEHDAKTFAEWGIDYLKYDNCNSDGSKPTVRYP 189

Query: 191 VMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESMISRADLNEPYA 250
           VMTRALMK+GRPIF SLCEWGD+HPALWG  +GNSWRTTNDI D+W SMIS AD+NE YA
Sbjct: 190 VMTRALMKSGRPIFHSLCEWGDMHPALWGSPVGNSWRTTNDIKDTWLSMISIADMNEVYA 249

Query: 251 DLARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITNT 310
           + ARPGGWNDPDMLEVGNGGMTKDEYIVHFS+WAISKAPLLLGCD+RN+TKET EI+ N 
Sbjct: 250 EHARPGGWNDPDMLEVGNGGMTKDEYIVHFSIWAISKAPLLLGCDIRNMTKETMEIVANK 309

Query: 311 EVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSGYRVALVLLNRGPWRSAITAQWEDIGIPP 370
           EVIA+NQDP GVQAKKVR EGDLEVWAGPLSGYRVAL+LLNRGP R++ITA WEDI IP 
Sbjct: 310 EVIAINQDPHGVQAKKVRMEGDLEVWAGPLSGYRVALLLLNRGPSRTSITALWEDIEIPA 369

Query: 371 NSNVQARDLWEHTTLKTIFVANLTATLDSHSCKLYILKPIS 412
           NS V+ARDLWEH TLK  FV NLTAT+DSH+CKLY+LKP++
Sbjct: 370 NSIVEARDLWEHQTLKQKFVGNLTATVDSHACKLYVLKPVA 410

[Close]

BLAST of Lag0040628 vs. TAIR 10
Match: AT5G08370.1 (alpha-galactosidase 2 )

HSP 1 Score: 535.4 bits (1378), Expect = 4.0e-152
Identity = 246/376 (65.43%), Postives = 298/376 (79.26%), Query Frame = 0


Query: 32  MTEIAADGELLRRNLLANGLGVTPPMGWNSWNHFACNINEKMIKETADAMVSTGLSKLGY 91
           +T+I ADG    R L+ NGL ++P MGWNSWNHF CNINE +IK+TADAMVS+GLS +GY
Sbjct: 19  LTQI-ADG-FQSRMLMNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSAIGY 78

Query: 92  EYVNIDDCWAEIARDDKGYLVPKKSTFPSGMKALADYVHSKGLKLGIYSDAGYFTCSKTM 151
           +Y+NIDDCW E+ RD +G LV K STFPSG+KAL+DYVHSKGLKLGIYSDAG  TCS+TM
Sbjct: 79  KYINIDDCWGELKRDSQGSLVAKASTFPSGIKALSDYVHSKGLKLGIYSDAGTLTCSQTM 138

Query: 152 PGSLGHEEQDAKTFASWGIDYLKYDNCNNGNIKPTIRYPVMTRALMKAGRPIFFSLCEWG 211
           PGSLGHEEQDAKTFASWGIDYLKYDNC N    P  RYP M++AL+ +GR IFFSLCEWG
Sbjct: 139 PGSLGHEEQDAKTFASWGIDYLKYDNCENTGTSPRERYPKMSKALLNSGRSIFFSLCEWG 198

Query: 212 DLHPALWGDKLGNSWRTTNDINDSWESMISRADLNEPYADLARPGGWNDPDMLEVGNGGM 271
              PA W   +GNSWRTT DI D+W+SM   AD N+ +A  ARPG WNDPDMLEVGNGGM
Sbjct: 199 QEDPATWAGDIGNSWRTTGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGGM 258

Query: 272 TKDEYIVHFSLWAISKAPLLLGCDLRNLTKETKEIITNTEVIAVNQDPLGVQAKKVRSEG 331
           TK+EY+ HFS+WA++KAPLL+GCDLR++ K T E+++N EVIAVNQD LG+Q KKV+ EG
Sbjct: 259 TKEEYMSHFSIWALAKAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVKKEG 318

Query: 332 DLEVWAGPLSGYRVALVLLNRGPWRSAITAQWEDIGIPPNSNVQARDLWEHTTLKTIFVA 391
           DLEVWAGPLS  RVA++L NRG   + ITA+W +IG+  +  V ARDLWEH+T   +   
Sbjct: 319 DLEVWAGPLSKKRVAVILWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYSCV-KK 378

Query: 392 NLTATLDSHSCKLYIL 408
            L+A ++ H+CK+Y L
Sbjct: 379 QLSALVEPHACKMYTL 391

[Close]

BLAST of Lag0040628 vs. TAIR 10
Match: AT5G08370.2 (alpha-galactosidase 2 )

HSP 1 Score: 535.0 bits (1377), Expect = 5.2e-152
Identity = 241/366 (65.85%), Postives = 292/366 (79.78%), Query Frame = 0


Query: 42  LRRNLLANGLGVTPPMGWNSWNHFACNINEKMIKETADAMVSTGLSKLGYEYVNIDDCWA 101
           + R L+ NGL ++P MGWNSWNHF CNINE +IK+TADAMVS+GLS +GY+Y+NIDDCW 
Sbjct: 1   MSRMLMNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSAIGYKYINIDDCWG 60

Query: 102 EIARDDKGYLVPKKSTFPSGMKALADYVHSKGLKLGIYSDAGYFTCSKTMPGSLGHEEQD 161
           E+ RD +G LV K STFPSG+KAL+DYVHSKGLKLGIYSDAG  TCS+TMPGSLGHEEQD
Sbjct: 61  ELKRDSQGSLVAKASTFPSGIKALSDYVHSKGLKLGIYSDAGTLTCSQTMPGSLGHEEQD 120

Query: 162 AKTFASWGIDYLKYDNCNNGNIKPTIRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDK 221
           AKTFASWGIDYLKYDNC N    P  RYP M++AL+ +GR IFFSLCEWG   PA W   
Sbjct: 121 AKTFASWGIDYLKYDNCENTGTSPRERYPKMSKALLNSGRSIFFSLCEWGQEDPATWAGD 180

Query: 222 LGNSWRTTNDINDSWESMISRADLNEPYADLARPGGWNDPDMLEVGNGGMTKDEYIVHFS 281
           +GNSWRTT DI D+W+SM   AD N+ +A  ARPG WNDPDMLEVGNGGMTK+EY+ HFS
Sbjct: 181 IGNSWRTTGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGGMTKEEYMSHFS 240

Query: 282 LWAISKAPLLLGCDLRNLTKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLS 341
           +WA++KAPLL+GCDLR++ K T E+++N EVIAVNQD LG+Q KKV+ EGDLEVWAGPLS
Sbjct: 241 IWALAKAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVKKEGDLEVWAGPLS 300

Query: 342 GYRVALVLLNRGPWRSAITAQWEDIGIPPNSNVQARDLWEHTTLKTIFVANLTATLDSHS 401
             RVA++L NRG   + ITA+W +IG+  +  V ARDLWEH+T   +    L+A ++ H+
Sbjct: 301 KKRVAVILWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYSCV-KKQLSALVEPHA 360

Query: 402 CKLYIL 408
           CK+Y L
Sbjct: 361 CKMYTL 365

[Close]

BLAST of Lag0040628 vs. TAIR 10
Match: AT3G56310.1 (Melibiase family protein )

HSP 1 Score: 486.5 bits (1251), Expect = 2.1e-137
Identity = 226/375 (60.27%), Postives = 275/375 (73.33%), Query Frame = 0


Query: 38  DGELLRRNLLANGLGVTPPMGWNSWNHFACNINEKMIKETADAMVSTGLSKLGYEYVNID 97
           D  +  R  L NGL  TP MGWNSWN FACNINE +IKETADA+VS+GL+ LGY +VNID
Sbjct: 56  DTSMYGRLQLNNGLARTPQMGWNSWNFFACNINETVIKETADALVSSGLADLGYIHVNID 115

Query: 98  DCWAEIARDDKGYLVPKKSTFPSGMKALADYVHSKGLKLGIYSDAGYFTCSKTMPGSLGH 157
           DCW+ + RD +G LVP   TFPSG+K LADYVHSKGLKLGIYSDAG FTC +  PGSL H
Sbjct: 116 DCWSNLLRDSEGQLVPHPETFPSGIKLLADYVHSKGLKLGIYSDAGVFTC-EVHPGSLFH 175

Query: 158 EEQDAKTFASWGIDYLKYDNCNNGNIKPTIRYPVMTRALMKAGRPIFFSLCEWGDLHPAL 217
           E  DA  FASWG+DYLKYDNC N  IKP  RYP M  AL   GR IF+SLCEWG   PAL
Sbjct: 176 EVDDADIFASWGVDYLKYDNCFNLGIKPIERYPPMRDALNATGRSIFYSLCEWGVDDPAL 235

Query: 218 WGDKLGNSWRTTNDINDSWESMISRADLNEPYADLARPGGWNDPDMLEVGNGGMTKDEYI 277
           W  ++GNSWRTT+DIND+W SM + ADLN  +A  A PGGWNDPDMLE+GNGGMT +EY 
Sbjct: 236 WAKEVGNSWRTTDDINDTWASMTTIADLNNKWAAYAGPGGWNDPDMLEIGNGGMTYEEYR 295

Query: 278 VHFSLWAISKAPLLLGCDLRNLTKETKEIITNTEVIAVNQDPLGVQAKKVRSEGD---LE 337
            HFS+WA+ KAPLL+GCD+RN+T ET EI++N E+IAVNQDPLGVQ +K+++ G+    +
Sbjct: 296 GHFSIWALMKAPLLIGCDVRNMTAETLEILSNKEIIAVNQDPLGVQGRKIQANGENDCQQ 355

Query: 338 VWAGPLSGYRVALVLLNRGPWRSAITAQWEDIGIPPNSNVQARDLWEHTTLKTIFVANLT 397
           VW+GPLSG R+ + L NR    + ITA W+ IG+    +V  RDLW+H  +      +  
Sbjct: 356 VWSGPLSGDRMVVALWNRCSEPATITASWDMIGLESTISVSVRDLWQHKDVTENTSGSFE 415

Query: 398 ATLDSHSCKLYILKP 410
           A +D+H C +Y+L P
Sbjct: 416 AQVDAHDCHMYVLTP 429

[Close]

BLAST of Lag0040628 vs. TAIR 10
Match: AT3G56310.2 (Melibiase family protein )

HSP 1 Score: 438.7 bits (1127), Expect = 5.1e-123
Identity = 212/375 (56.53%), Postives = 258/375 (68.80%), Query Frame = 0


Query: 38  DGELLRRNLLANGLGVTPPMGWNSWNHFACNINEKMIKETADAMVSTGLSKLGYEYVNID 97
           D  +  R  L NGL  TP MGWNSWN FACNINE +IKETADA+VS+GL+ LGY +VNI 
Sbjct: 56  DTSMYGRLQLNNGLARTPQMGWNSWNFFACNINETVIKETADALVSSGLADLGYIHVNI- 115

Query: 98  DCWAEIARDDKGYLVPKKSTFPSGMKALADYVHSKGLKLGIYSDAGYFTCSKTMPGSLGH 157
                                  G+K LADYVHSKGLKLGIYSDAG FTC +  PGSL H
Sbjct: 116 -----------------------GIKLLADYVHSKGLKLGIYSDAGVFTC-EVHPGSLFH 175

Query: 158 EEQDAKTFASWGIDYLKYDNCNNGNIKPTIRYPVMTRALMKAGRPIFFSLCEWGDLHPAL 217
           E  DA  FASWG+DYLKYDNC N  IKP  RYP M  AL   GR IF+SLCEWG   PAL
Sbjct: 176 EVDDADIFASWGVDYLKYDNCFNLGIKPIERYPPMRDALNATGRSIFYSLCEWGVDDPAL 235

Query: 218 WGDKLGNSWRTTNDINDSWESMISRADLNEPYADLARPGGWNDPDMLEVGNGGMTKDEYI 277
           W  ++GNSWRTT+DIND+W SM + ADLN  +A  A PGGWNDPDMLE+GNGGMT +EY 
Sbjct: 236 WAKEVGNSWRTTDDINDTWASMTTIADLNNKWAAYAGPGGWNDPDMLEIGNGGMTYEEYR 295

Query: 278 VHFSLWAISKAPLLLGCDLRNLTKETKEIITNTEVIAVNQDPLGVQAKKVRSEGD---LE 337
            HFS+WA+ KAPLL+GCD+RN+T ET EI++N E+IAVNQDPLGVQ +K+++ G+    +
Sbjct: 296 GHFSIWALMKAPLLIGCDVRNMTAETLEILSNKEIIAVNQDPLGVQGRKIQANGENDCQQ 355

Query: 338 VWAGPLSGYRVALVLLNRGPWRSAITAQWEDIGIPPNSNVQARDLWEHTTLKTIFVANLT 397
           VW+GPLSG R+ + L NR    + ITA W+ IG+    +V  RDLW+H  +      +  
Sbjct: 356 VWSGPLSGDRMVVALWNRCSEPATITASWDMIGLESTISVSVRDLWQHKDVTENTSGSFE 405

Query: 398 ATLDSHSCKLYILKP 410
           A +D+H C +Y+L P
Sbjct: 416 AQVDAHDCHMYVLTP 405

The following BLAST results are available for this feature:

BLAST of Lag0040628 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6573765.1	6.2e-232	93.93	Alpha-galactosidase 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7012838.1	8.1e-232	93.93	Alpha-galactosidase 1 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022966950.1	2.4e-231	93.45	alpha-galactosidase 1-like [Cucurbita maxima]	[more]
XP_023541761.1	6.9e-231	93.20	alpha-galactosidase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022945946.1	6.9e-231	93.45	alpha-galactosidase 1-like [Cucurbita moschata]	[more]

BLAST of Lag0040628 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FT97	2.0e-196	78.55	Alpha-galactosidase 1 OS=Arabidopsis thaliana OX=3702 GN=AGAL1 PE=2 SV=1	[more]
Q42656	5.0e-160	68.39	Alpha-galactosidase OS=Coffea arabica OX=13443 PE=1 SV=1	[more]
P14749	1.9e-151	60.64	Alpha-galactosidase OS=Cyamopsis tetragonoloba OX=3832 PE=1 SV=1	[more]
Q8RX86	5.6e-151	65.43	Alpha-galactosidase 2 OS=Arabidopsis thaliana OX=3702 GN=AGAL2 PE=1 SV=1	[more]
Q9FXT4	9.6e-143	62.40	Alpha-galactosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0493600 PE=...	[more]

BLAST of Lag0040628 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HT03	1.1e-231	93.45	Alpha-galactosidase OS=Cucurbita maxima OX=3661 GN=LOC111466509 PE=3 SV=1	[more]
A0A6J1G2A9	3.3e-231	93.45	Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111450037 PE=3 SV=1	[more]
A0A6J1CC22	1.9e-226	91.08	Alpha-galactosidase OS=Momordica charantia OX=3673 GN=LOC111010280 PE=3 SV=1	[more]
Q2MK92	7.9e-225	90.80	Alpha-galactosidase OS=Cucumis sativus OX=3659 PE=2 SV=1	[more]
A0A1S3BDT4	7.9e-225	91.30	Alpha-galactosidase OS=Cucumis melo OX=3656 GN=LOC103488793 PE=3 SV=1	[more]

BLAST of Lag0040628 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G08380.1	1.4e-197	78.55	alpha-galactosidase 1	[more]
AT5G08370.1	4.0e-152	65.43	alpha-galactosidase 2	[more]
AT5G08370.2	5.2e-152	65.85	alpha-galactosidase 2	[more]
AT3G56310.1	2.1e-137	60.27	Melibiase family protein	[more]
AT3G56310.2	5.1e-123	56.53	Melibiase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002241	Glycoside hydrolase, family 27	PRINTS	PR00740	GLHYDRLASE27	coord: 126..147 score: 63.41 coord: 274..295 score: 52.27 coord: 161..178 score: 76.39 coord: 188..206 score: 55.79 coord: 253..272 score: 69.75 coord: 49..68 score: 69.5 coord: 85..100 score: 65.94
IPR002241	Glycoside hydrolase, family 27	PFAM	PF16499	Melibiase_2	coord: 54..318 e-value: 4.3E-81 score: 272.2
IPR002241	Glycoside hydrolase, family 27	PANTHER	PTHR11452	ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASE	coord: 11..410
IPR002241	Glycoside hydrolase, family 27	CDD	cd14792	GH27	coord: 55..318 e-value: 6.14553E-160 score: 449.313
IPR041233	Alpha galactosidase, C-terminal beta sandwich domain	PFAM	PF17801	Melibiase_C	coord: 330..407 e-value: 5.6E-18 score: 64.8
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 47..320 e-value: 1.8E-133 score: 446.2
IPR013780	Glycosyl hydrolase, all-beta	GENE3D	2.60.40.1180		coord: 321..410 e-value: 2.5E-26 score: 93.5
None	No IPR available	PANTHER	PTHR11452:SF82	ALPHA-GALACTOSIDASE	coord: 11..410
None	No IPR available	SUPERFAMILY	51011	Glycosyl hydrolase domain	coord: 313..407
IPR000111	Glycoside hydrolase family 27/36, conserved site	PROSITE	PS00512	ALPHA_GALACTOSIDASE	coord: 90..106
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 48..319

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0040628.1	Lag0040628.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0009505	plant-type cell wall
molecular_function	GO:0052692	raffinose alpha-galactosidase activity
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds