Lag0040625 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0040625
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionAlpha-galactosidase
Locationchr13: 6676493 .. 6680306 (-)
RNA-Seq ExpressionLag0040625
SyntenyLag0040625
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTTTCCCTCACTTCCGCTTTCATCTCCAGCTTCGACTATGGTTCTCTTCTACTCCGTCCTTTTCTGGACGTTCTTGTTCGGTAGCAACAGCGATGGCGGAGCGGTGGCCGGAGCGACTCGATCGAGACCTCTTCGGTTTGCCGCCGAGTTTGATTCTGATTCCACCAGGAGAGTTCTCCTCAATAATGGCCTCGCTCTAACTCCTCCGATGGGGTAAGTCTTACGCTTCGATCATGAGTTTAGTCTCTTTCTCAGGAGGATCGATCAGATTGAGTATAATGTAGTCTGTTCGTTTAATTGTCGATCTTCTCGGTTTCGTTCAATAGTGTTTAGTTATTGATAATTTGATGGTGATGGATTGCTGATTTTTGTAATGAGCACAGATGGAATAGCTGGAACCATTTTCAATGTAATATAAACGAGAATTTAATCAAGGAAACAGGTGAGATTTTGATTTGAAGGCGTCTTTCTGTTTGGATTTGTGTACAGTGACTGAGATACACATTCTATTTCTTTTGCTAAGCGGATGCAATGGTATCCAGTGGCCTTGCTGCATTAGGATATGAATATATCAATTTAGGTGATGATTATATTTCTTTCTTTTTACATTTCTTTGATTTTTCATTTTTATGCTTTCTGCTTTTGACATTAATATAATCTTATTTCGATTTGAGCTAGATGATTGTTGGGCTGAACTTAACAGGGATTCTAAGGTAAATTTCAATATCCTATTATTTCCTGTCTCGGATTTCTATGAACATCTCTGAATTTGCATTAGATTATGTGAAGATTATACAAACTAGCAATAAGTTTGGCGTTGAAGATTCCAGCGATATTCTCAGGGTAATTTGGTTGCTAAAGTTTCAACATTCCCTTCTGGTATCAAGGCCTTGGCAGATTATGTTCATAGAAAAGGGCTCAAGCTCGGAATTTATTCTGATGCTGGGTAAGTTTATCCACTCATCACATCGCTGTTCATCAAAATGCTGCAGATAGTTTTATTTGCTGAAATATTACTATTCCCACTTTTTGGCTTACGGAAATACCACTTTTAGGCCTGTTTTAGTTAGATCCTCTTTAGCTAAGTTTATATAATTTCTTCAGGATCCAGACTTGCAGCAAGAAGATGCCAGGTTCCCTTGGTCACGAAGAACAGGATGCAAAAACCTTCGCCTCATGGGTATTTTCGTAGCCTTATACACTTAACTACTTAAATTCTTGTTTATGTCACTATAGATAGTCTGAAATTGAGATACCTTATTTCTCTTTCAGAAACTGATATATACACTATTGTCTAGGGGATCGATTACTTGAAGTATGATAACTGTGAAAATACTGGTACAAGCCCAAAAGAAAGGTAGGATCACATTTCTATGTTTAATTATAAAAAATTCCAAATAACTGCAACAATCCCTGGGGCACAGGTATCCGAAGATGACTAAAGCTTTACAACAATCTGGGAGACCCATTCTTTTTTCTTTATGCGAATGGTTAGCAATCTGTCTCCACAAAAAAATATTCCACCTCTTTTTTTTCTCTCTCTAATCCTTCTCCATTCAAAAGCACAGATAAAGTTCCTCTCTTAAGTTTTTCCTTATTTAGGGGACAAGAAGATCCAGCAACTTGGGCAGTAAATGTAGGAAATAGTTGGAGAACTACATCGGATATTCAAGATAACTGGAATAGGTATTAAGCGTATATTTTATCATCATTTCTAGACACGTGCAACATTTTGAAAAACTTAGTCTAAATTATTTGATTTGTTTCTTTTTTCCCCCATATATGAATATGCCTGCATTGAAGTATGATATTTTCAAATCCAAAATACTTTCTTTACCAAGAAACTGATGCTGCTACTTAATGGTACAGCATGACAACCATTGCTGATCAGAATGACAAATGGGCATCTTATGCTAAACCTGGAGGATGGAATGGTAAATTCCAGAATTGTAGTTCAATATGAAAAAGATATCCTTGTGTATAGAGTAATATTGGCCGCCGGTTAGCTTCTCAGTTGGCCCCTTTTCATATCTCTTAGTACTAATTAAAGATTGATAATAATTGAAGAAGTTAACTCAATTCTGATTCTGAACAGAGGGCTAGACGATATCCCCTGCTGGCATTAGCCACCAACTGTTGAAACTCAGCCTCGAACAATCTTGTTTGAGGAAAACTATTTGAAAGGAAAAGTAAAATATAGAAGTCTGAATTTTTATTTTTATTTTTTTATGGAAATGGTCTGAATATTCATAGTTATTATCAAATGACTTATTTCTGTGATTATGATTAAATAACTTTTTTAATGCATTTAAAATTTTTTCCATTTGGCTAATCTCTATGGAAGACATGGAAATTAAACTGTCATAAATGCTAAAGAAAATAATCATAGTTTACAATTTTCTTTGTTCAGATCCTGACATGCTTGAAGTGGGGAATGGAGGGATGAAAACAGCAGAATATCGTTCTCATTTTAGCATTTGGGCACTGGCAAAAGTAAGTACCATTGAGTAACACTTTTCCAGAATCAAGGTTTTAGTTTAGATAGGTAATATCTTGTATCATATTAAGACATATTCGGAATCCAAGTTCATTAAATAACTTAACCATTTCAAAAAACTATATTAAACTAGGATTGGATATCTTTGCTTGATTTGTGGTTTGAGTATATAATTTTGTGTGCTTCATAAAATATCTTATTAATTTATAATCGATTCCTTTCTGTTCTTCATATGTGAAGGCTCCTCTGCTGATTGGATGTGACATCAGATCAATGGACAATATCACCTTAAAATTGTTAAGTAATAAGGAGGTTATTGCAGTTAATCAGGGTAAGTAAAAGCCAAACTATTTCTGCTAATTCATAGAGAACTTTGAAACAGATAACCAAACATAGTAATAATTTCTCTCAATAAAATGGAAAGATTAATCATTAACCAAACAAGACATTATGTGTGCTCCCATCTACCATGCAGATAAACTTGGAGTTCAAGGGAAGAAGGTCAATAAACTTGGAGATCTTGAGGTAATTTTTCATCATCTAACACATCCCAAAAGAAACAAAAGATAGAAGAAAAAAAAGTAAAAGATCTTTACTCTGAAATTTTCTACACAAAAATGATGCAGGTTTGGGCAGGGCCGCTGAGTGGTAAAAGAGTCGCTGTGGTTCTATGGAACAGAGGTTTATGGAGAGCCAATATCACTGCATCTTGGTCAGATATAGGTTTGAATCCATCAACTACTGTTACTGCTCGAGACTTATGGGAGGTAAGTTATGAATAACATGTTTGAGAGTGATTTTAAAATAACCAAAATCGCCCGTCATATCTTTTTTAGTGAAAAATGGCATGAAAAAAGTGATTTCTAGTCATTCCAAAATCACTCCCAAACATGTTTCAAGTGTTTTATGACATTAGATTCTGCAGTAAAAAGTACTTTTAAAATTTCTACCAGTCGATGAAAGCAAAGATTTTGAAGTAAGCACATAATTAGTACCTTTTCTGAAAGAACGCTTCACTTAAAAGTATTGCTCTCGCATCTCAAACGTCGTTCGTAACTCACACGAAGAGATGTGCATTATTTCTTTGTACATACATCGAACTCGTGCCAAAACTTTCTGACTGATTATTCATTCCTACAATAGCACTCCAACCAGGTGGTTCAACATCAACTCACTGCTCAAGTAGACTCTCATGACTGTAAGATGTATGTTCTCACACCCCACTAAGAGCCAAAATGGAAACACTGCAAATGTTCTTGTGAATGATTGTTGTCTTCACTTCAGCTACATGTTCTTCCACTCTATGCTGTGA

mRNA sequence

ATGGCTTTTCCCTCACTTCCGCTTTCATCTCCAGCTTCGACTATGGTTCTCTTCTACTCCGTCCTTTTCTGGACGTTCTTGTTCGGTAGCAACAGCGATGGCGGAGCGGTGGCCGGAGCGACTCGATCGAGACCTCTTCGGTTTGCCGCCGAGTTTGATTCTGATTCCACCAGGAGAGTTCTCCTCAATAATGGCCTCGCTCTAACTCCTCCGATGGGATGGAATAGCTGGAACCATTTTCAATGTAATATAAACGAGAATTTAATCAAGGAAACAGCGGATGCAATGGTATCCAGTGGCCTTGCTGCATTAGGATATGAATATATCAATTTAGATGATTGTTGGGCTGAACTTAACAGGGATTCTAAGGGTAATTTGGTTGCTAAAGTTTCAACATTCCCTTCTGGTATCAAGGCCTTGGCAGATTATGTTCATAGAAAAGGGCTCAAGCTCGGAATTTATTCTGATGCTGGGATCCAGACTTGCAGCAAGAAGATGCCAGGTTCCCTTGGTCACGAAGAACAGGATGCAAAAACCTTCGCCTCATGGGGGATCGATTACTTGAAGTATGATAACTGTGAAAATACTGGTACAAGCCCAAAAGAAAGGTATCCGAAGATGACTAAAGCTTTACAACAATCTGGGAGACCCATTCTTTTTTCTTTATGCGAATGGGGACAAGAAGATCCAGCAACTTGGGCAGTAAATGTAGGAAATAGTTGGAGAACTACATCGGATATTCAAGATAACTGGAATAGCATGACAACCATTGCTGATCAGAATGACAAATGGGCATCTTATGCTAAACCTGGAGGATGGAATGATCCTGACATGCTTGAAGTGGGGAATGGAGGGATGAAAACAGCAGAATATCGTTCTCATTTTAGCATTTGGGCACTGGCAAAAGCTCCTCTGCTGATTGGATGTGACATCAGATCAATGGACAATATCACCTTAAAATTGTTAAGTAATAAGGAGGTTATTGCAGTTAATCAGGATAAACTTGGAGTTCAAGGGAAGAAGGTCAATAAACTTGGAGATCTTGAGGTTTGGGCAGGGCCGCTGAGTGGTAAAAGAGTCGCTGTGGTTCTATGGAACAGAGGTTTATGGAGAGCCAATATCACTGCATCTTGGTCAGATATAGGTTTGAATCCATCAACTACTGTTACTGCTCGAGACTTATGGGAGCACTCCAACCAGGTGGTTCAACATCAACTCACTGCTCAAGTAGACTCTCATGACTGTAAGATCTACATGTTCTTCCACTCTATGCTGTGA

Coding sequence (CDS)

ATGGCTTTTCCCTCACTTCCGCTTTCATCTCCAGCTTCGACTATGGTTCTCTTCTACTCCGTCCTTTTCTGGACGTTCTTGTTCGGTAGCAACAGCGATGGCGGAGCGGTGGCCGGAGCGACTCGATCGAGACCTCTTCGGTTTGCCGCCGAGTTTGATTCTGATTCCACCAGGAGAGTTCTCCTCAATAATGGCCTCGCTCTAACTCCTCCGATGGGATGGAATAGCTGGAACCATTTTCAATGTAATATAAACGAGAATTTAATCAAGGAAACAGCGGATGCAATGGTATCCAGTGGCCTTGCTGCATTAGGATATGAATATATCAATTTAGATGATTGTTGGGCTGAACTTAACAGGGATTCTAAGGGTAATTTGGTTGCTAAAGTTTCAACATTCCCTTCTGGTATCAAGGCCTTGGCAGATTATGTTCATAGAAAAGGGCTCAAGCTCGGAATTTATTCTGATGCTGGGATCCAGACTTGCAGCAAGAAGATGCCAGGTTCCCTTGGTCACGAAGAACAGGATGCAAAAACCTTCGCCTCATGGGGGATCGATTACTTGAAGTATGATAACTGTGAAAATACTGGTACAAGCCCAAAAGAAAGGTATCCGAAGATGACTAAAGCTTTACAACAATCTGGGAGACCCATTCTTTTTTCTTTATGCGAATGGGGACAAGAAGATCCAGCAACTTGGGCAGTAAATGTAGGAAATAGTTGGAGAACTACATCGGATATTCAAGATAACTGGAATAGCATGACAACCATTGCTGATCAGAATGACAAATGGGCATCTTATGCTAAACCTGGAGGATGGAATGATCCTGACATGCTTGAAGTGGGGAATGGAGGGATGAAAACAGCAGAATATCGTTCTCATTTTAGCATTTGGGCACTGGCAAAAGCTCCTCTGCTGATTGGATGTGACATCAGATCAATGGACAATATCACCTTAAAATTGTTAAGTAATAAGGAGGTTATTGCAGTTAATCAGGATAAACTTGGAGTTCAAGGGAAGAAGGTCAATAAACTTGGAGATCTTGAGGTTTGGGCAGGGCCGCTGAGTGGTAAAAGAGTCGCTGTGGTTCTATGGAACAGAGGTTTATGGAGAGCCAATATCACTGCATCTTGGTCAGATATAGGTTTGAATCCATCAACTACTGTTACTGCTCGAGACTTATGGGAGCACTCCAACCAGGTGGTTCAACATCAACTCACTGCTCAAGTAGACTCTCATGACTGTAAGATCTACATGTTCTTCCACTCTATGCTGTGA

Protein sequence

MAFPSLPLSSPASTMVLFYSVLFWTFLFGSNSDGGAVAGATRSRPLRFAAEFDSDSTRRVLLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLDDCWAELNRDSKGNLVAKVSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMKTAEYRSHFSIWALAKAPLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVNKLGDLEVWAGPLSGKRVAVVLWNRGLWRANITASWSDIGLNPSTTVTARDLWEHSNQVVQHQLTAQVDSHDCKIYMFFHSML
Homology
BLAST of Lag0040625 vs. NCBI nr
Match: XP_023541567.1 (alpha-galactosidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 805.4 bits (2079), Expect = 2.3e-229
Identity = 384/412 (93.20%), Postives = 396/412 (96.12%), Query Frame = 0

Query: 8   LSSPASTMVLFYSVLFWTFLFGSNSDGGAVAGATRSRPLRFAAEFDSDSTRRVLLNNGLA 67
           +SSPAS M+L YSVLFWTFLF SN +GGA+AGATRSRPLRFAAEFD DS RRVLLNNGLA
Sbjct: 2   VSSPASIMLLLYSVLFWTFLFSSNGNGGALAGATRSRPLRFAAEFDYDSPRRVLLNNGLA 61

Query: 68  LTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLDDCWAELNRDSKGNLV 127
           LTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGY+YINLDDCWAELNRD+KGNLV
Sbjct: 62  LTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYQYINLDDCWAELNRDAKGNLV 121

Query: 128 AKVSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGIDY 187
            K STFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGIDY
Sbjct: 122 PKSSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGIDY 181

Query: 188 LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI 247
           LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI
Sbjct: 182 LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI 241

Query: 248 QDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMKTAEYRSHFSIWALAKAPLLI 307
           QDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGM TAEYRSHFSIWALAKAPLLI
Sbjct: 242 QDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLI 301

Query: 308 GCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVNKLGDLEVWAGPLSGKRVAVVLWNR 367
           GCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKV  LGDLEVWAG LSGKRVAVVLWNR
Sbjct: 302 GCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYILGDLEVWAGALSGKRVAVVLWNR 361

Query: 368 GLWRANITASWSDIGLNPSTTVTARDLWEHSNQVVQHQLTAQVDSHDCKIYM 420
           G +RA ITASWS IGL+PSTTVTARDLWEHS+QVVQHQ+TAQVD HDCK+Y+
Sbjct: 362 GFYRAKITASWSAIGLSPSTTVTARDLWEHSSQVVQHQITAQVDPHDCKMYV 413

BLAST of Lag0040625 vs. NCBI nr
Match: XP_022944991.1 (alpha-galactosidase-like [Cucurbita moschata])

HSP 1 Score: 803.9 bits (2075), Expect = 6.6e-229
Identity = 383/412 (92.96%), Postives = 395/412 (95.87%), Query Frame = 0

Query: 8   LSSPASTMVLFYSVLFWTFLFGSNSDGGAVAGATRSRPLRFAAEFDSDSTRRVLLNNGLA 67
           +SSPAS M+  YSVLFWTFLF SN +GGA+AGATRSRPLRFAAEFD DS RRVLLNNGLA
Sbjct: 2   VSSPASIMLFLYSVLFWTFLFSSNGNGGAMAGATRSRPLRFAAEFDYDSPRRVLLNNGLA 61

Query: 68  LTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLDDCWAELNRDSKGNLV 127
           LTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGY+YINLDDCWAELNRD+KGNLV
Sbjct: 62  LTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYQYINLDDCWAELNRDAKGNLV 121

Query: 128 AKVSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGIDY 187
            K STFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGIDY
Sbjct: 122 PKSSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGIDY 181

Query: 188 LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI 247
           LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI
Sbjct: 182 LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI 241

Query: 248 QDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMKTAEYRSHFSIWALAKAPLLI 307
           QDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGM TAEYRSHFSIWALAKAPLLI
Sbjct: 242 QDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLI 301

Query: 308 GCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVNKLGDLEVWAGPLSGKRVAVVLWNR 367
           GCDIRSMDNITLKLLSNK VIAVNQDKLGVQGKKV KLGDLEVWAG LSGKRVAVVLWNR
Sbjct: 302 GCDIRSMDNITLKLLSNKLVIAVNQDKLGVQGKKVYKLGDLEVWAGALSGKRVAVVLWNR 361

Query: 368 GLWRANITASWSDIGLNPSTTVTARDLWEHSNQVVQHQLTAQVDSHDCKIYM 420
           G +RA ITASWS IGL+PSTTVTARDLWEHS+QVVQHQ+TAQVD HDCK+Y+
Sbjct: 362 GFYRAKITASWSAIGLSPSTTVTARDLWEHSSQVVQHQITAQVDPHDCKMYV 413

BLAST of Lag0040625 vs. NCBI nr
Match: XP_022966963.1 (alpha-galactosidase-like [Cucurbita maxima])

HSP 1 Score: 803.5 bits (2074), Expect = 8.7e-229
Identity = 383/412 (92.96%), Postives = 394/412 (95.63%), Query Frame = 0

Query: 8   LSSPASTMVLFYSVLFWTFLFGSNSDGGAVAGATRSRPLRFAAEFDSDSTRRVLLNNGLA 67
           +SSPAS M+L YSVLFWTF F SN +GGA+AGATRSRPLRFA EFD DS RRVLLNNGLA
Sbjct: 2   VSSPASIMLLLYSVLFWTFFFSSNGNGGAMAGATRSRPLRFATEFDYDSPRRVLLNNGLA 61

Query: 68  LTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLDDCWAELNRDSKGNLV 127
           LTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGY+YINLDDCWAELNRD+KGNLV
Sbjct: 62  LTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYQYINLDDCWAELNRDAKGNLV 121

Query: 128 AKVSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGIDY 187
            K STFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSL HEEQDAKTFASWGIDY
Sbjct: 122 PKSSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLDHEEQDAKTFASWGIDY 181

Query: 188 LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI 247
           LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI
Sbjct: 182 LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI 241

Query: 248 QDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMKTAEYRSHFSIWALAKAPLLI 307
           QDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGM TAEYRSHFSIWALAKAPLLI
Sbjct: 242 QDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLI 301

Query: 308 GCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVNKLGDLEVWAGPLSGKRVAVVLWNR 367
           GCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKV KLGDLEVWAG LSGKRVAVVLWNR
Sbjct: 302 GCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKLGDLEVWAGTLSGKRVAVVLWNR 361

Query: 368 GLWRANITASWSDIGLNPSTTVTARDLWEHSNQVVQHQLTAQVDSHDCKIYM 420
           G +RA ITASWS IGL+PSTTVTARDLWEHS+QVVQHQLTAQVD HDCK+Y+
Sbjct: 362 GFYRAKITASWSAIGLSPSTTVTARDLWEHSSQVVQHQLTAQVDPHDCKMYV 413

BLAST of Lag0040625 vs. NCBI nr
Match: KAG6573784.1 (Alpha-galactosidase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 797.3 bits (2058), Expect = 6.2e-227
Identity = 381/411 (92.70%), Postives = 393/411 (95.62%), Query Frame = 0

Query: 4   PSLPLSSPASTMVLFYSVLFWTFLFGSNSDGGAVAGATRSRPLRFAAEFDSDSTRRVLLN 63
           PS  +SSPAS M+  YSVLFWTFLF SN +GGA+AGATRSRPLRFAAEFD DS RRVLLN
Sbjct: 271 PSAMVSSPASIMLFLYSVLFWTFLFSSNGNGGAMAGATRSRPLRFAAEFDYDSPRRVLLN 330

Query: 64  NGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLDDCWAELNRDSK 123
           NGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGY+YINLDDCWAELNRD+K
Sbjct: 331 NGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYQYINLDDCWAELNRDAK 390

Query: 124 GNLVAKVSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASW 183
           GNLV K STFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASW
Sbjct: 391 GNLVPKSSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASW 450

Query: 184 GIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRT 243
           GIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRT
Sbjct: 451 GIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRT 510

Query: 244 TSDIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMKTAEYRSHFSIWALAKA 303
           TSDIQD+WNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGM TAEYRSHFSIWALAKA
Sbjct: 511 TSDIQDDWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKA 570

Query: 304 PLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVNKLGDLEVWAGPLSGKRVAVV 363
           PLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKV KLGDLEVWAG LSGKRVAVV
Sbjct: 571 PLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVFKLGDLEVWAGALSGKRVAVV 630

Query: 364 LWNRGLWRANITASWSDIGLNPSTTVTARDLWEHSNQVVQHQLTAQVDSHD 415
           LWNRG +RA ITASWS IGL+PST+VTARDLWEHS+QVVQHQ+TAQVD HD
Sbjct: 631 LWNRGFYRAKITASWSAIGLSPSTSVTARDLWEHSSQVVQHQITAQVDPHD 681

BLAST of Lag0040625 vs. NCBI nr
Match: XP_038892679.1 (alpha-galactosidase-like isoform X2 [Benincasa hispida])

HSP 1 Score: 795.4 bits (2053), Expect = 2.4e-226
Identity = 384/419 (91.65%), Postives = 394/419 (94.03%), Query Frame = 0

Query: 1   MAFPSLPLSSPASTMVLFYSVLFWTFLFGSNSDGGAVAGATRSRPLRFAAEFDSDSTRRV 60
           MA P   LSSP ST+ L  SVLFW FL GSN  GGAVAGA RSR LRFAA FDS STRRV
Sbjct: 1   MASPPFLLSSPPSTIFLLCSVLFWMFLLGSN--GGAVAGAIRSRSLRFAAGFDSVSTRRV 60

Query: 61  LLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLDDCWAELNR 120
           LLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLA+LGYEYINLDDCWAELNR
Sbjct: 61  LLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLASLGYEYINLDDCWAELNR 120

Query: 121 DSKGNLVAKVSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTF 180
           DSKGNLVAK STFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHE+QDA+TF
Sbjct: 121 DSKGNLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEQQDAETF 180

Query: 181 ASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNS 240
           ASWGIDYLKYDNCENTGTSPKERYPKM+KALQQSGRPILFSLCEWGQEDPATWA+NVGNS
Sbjct: 181 ASWGIDYLKYDNCENTGTSPKERYPKMSKALQQSGRPILFSLCEWGQEDPATWAINVGNS 240

Query: 241 WRTTSDIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMKTAEYRSHFSIWAL 300
           WRTTSDIQDNW SMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGM T EYRSHFSIWAL
Sbjct: 241 WRTTSDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTVEYRSHFSIWAL 300

Query: 301 AKAPLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVNKLGDLEVWAGPLSGKRV 360
           AKAPLLIGCDIRSMDNI LKLLSNKEVIAVNQDKLGVQGKKV K GDLEVWAGPLSGKRV
Sbjct: 301 AKAPLLIGCDIRSMDNIALKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRV 360

Query: 361 AVVLWNRGLWRANITASWSDIGLNPSTTVTARDLWEHSNQVVQHQLTAQVDSHDCKIYM 420
           AVVLWNRGLWRANITASWSDIGL+ STTVTARDLWEHS+QVVQH LTAQVDSHDCK+Y+
Sbjct: 361 AVVLWNRGLWRANITASWSDIGLSTSTTVTARDLWEHSSQVVQHHLTAQVDSHDCKMYV 417

BLAST of Lag0040625 vs. ExPASy Swiss-Prot
Match: Q8RX86 (Alpha-galactosidase 2 OS=Arabidopsis thaliana OX=3702 GN=AGAL2 PE=1 SV=1)

HSP 1 Score: 622.9 bits (1605), Expect = 2.8e-177
Identity = 289/361 (80.06%), Postives = 318/361 (88.09%), Query Frame = 0

Query: 59  RVLLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLDDCWAEL 118
           R+L+NNGLAL+P MGWNSWNHFQCNINE LIK+TADAMVSSGL+A+GY+YIN+DDCW EL
Sbjct: 29  RMLMNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSAIGYKYINIDDCWGEL 88

Query: 119 NRDSKGNLVAKVSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAK 178
            RDS+G+LVAK STFPSGIKAL+DYVH KGLKLGIYSDAG  TCS+ MPGSLGHEEQDAK
Sbjct: 89  KRDSQGSLVAKASTFPSGIKALSDYVHSKGLKLGIYSDAGTLTCSQTMPGSLGHEEQDAK 148

Query: 179 TFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVG 238
           TFASWGIDYLKYDNCENTGTSP+ERYPKM+KAL  SGR I FSLCEWGQEDPATWA ++G
Sbjct: 149 TFASWGIDYLKYDNCENTGTSPRERYPKMSKALLNSGRSIFFSLCEWGQEDPATWAGDIG 208

Query: 239 NSWRTTSDIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMKTAEYRSHFSIW 298
           NSWRTT DIQDNW SMT IADQND+WASYA+PG WNDPDMLEVGNGGM   EY SHFSIW
Sbjct: 209 NSWRTTGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGGMTKEEYMSHFSIW 268

Query: 299 ALAKAPLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVNKLGDLEVWAGPLSGK 358
           ALAKAPLLIGCD+RSMD +T +LLSNKEVIAVNQDKLG+QGKKV K GDLEVWAGPLS K
Sbjct: 269 ALAKAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVKKEGDLEVWAGPLSKK 328

Query: 359 RVAVVLWNRGLWRANITASWSDIGLNPSTTVTARDLWEHSN-QVVQHQLTAQVDSHDCKI 418
           RVAV+LWNRG   ANITA W++IGLN S  V ARDLWEHS    V+ QL+A V+ H CK+
Sbjct: 329 RVAVILWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYSCVKKQLSALVEPHACKM 388

BLAST of Lag0040625 vs. ExPASy Swiss-Prot
Match: P14749 (Alpha-galactosidase OS=Cyamopsis tetragonoloba OX=3832 PE=1 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 4.4e-175
Identity = 284/386 (73.58%), Postives = 322/386 (83.42%), Query Frame = 0

Query: 35  GAVAGATRSRPLRFAAEFDSDSTRRVLLNNGLALTPPMGWNSWNHFQCNINENLIKETAD 94
           G+  G    +  R +AE +  + RR L  NGL  TPPMGWNSWNHF C+INEN+++ETAD
Sbjct: 21  GSEGGRLLEKKNRTSAEAEHYNVRRYLAENGLGQTPPMGWNSWNHFGCDINENVVRETAD 80

Query: 95  AMVSSGLAALGYEYINLDDCWAELNRDSKGNLVAKVSTFPSGIKALADYVHRKGLKLGIY 154
           AMVS+GLAALGY+YINLDDCWAELNRDS+GN+V   + FPSGIKALADYVH KGLKLG+Y
Sbjct: 81  AMVSTGLAALGYQYINLDDCWAELNRDSEGNMVPNAAAFPSGIKALADYVHSKGLKLGVY 140

Query: 155 SDAGIQTCSKKMPGSLGHEEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQS 214
           SDAG QTCSK+MPGSLGHEEQDAKTFASWG+DYLKYDNCEN G S KERYP M KAL  S
Sbjct: 141 SDAGNQTCSKRMPGSLGHEEQDAKTFASWGVDYLKYDNCENLGISVKERYPPMGKALLSS 200

Query: 215 GRPILFSLCEWGQEDPATWAVNVGNSWRTTSDIQDNWNSMTTIADQNDKWASYAKPGGWN 274
           GRPI FS+CEWG EDP  WA ++GNSWRTT DI+DNWNSMT+IAD NDKWASYA PGGWN
Sbjct: 201 GRPIFFSMCEWGWEDPQIWAKSIGNSWRTTGDIEDNWNSMTSIADSNDKWASYAGPGGWN 260

Query: 275 DPDMLEVGNGGMKTAEYRSHFSIWALAKAPLLIGCDIRSMDNITLKLLSNKEVIAVNQDK 334
           DPDMLEVGNGGM T EYRSHFSIWALAKAPLL+GCDIR+MD+ T +L+SN EVIAVNQDK
Sbjct: 261 DPDMLEVGNGGMTTEEYRSHFSIWALAKAPLLVGCDIRAMDDTTHELISNAEVIAVNQDK 320

Query: 335 LGVQGKKVNKLGDLEVWAGPLSGKRVAVVLWNRGLWRANITASWSDIGLNPSTTVTARDL 394
           LGVQGKKV    DLEVWAGPLS  +VAV+LWNR   RA +TASWSDIGL   TTV ARDL
Sbjct: 321 LGVQGKKVKSTNDLEVWAGPLSDNKVAVILWNRSSSRATVTASWSDIGLQQGTTVDARDL 380

Query: 395 WEHSNQ-VVQHQLTAQVDSHDCKIYM 420
           WEHS Q +V  +++A++DSH CK+Y+
Sbjct: 381 WEHSTQSLVSGEISAEIDSHACKMYV 406

BLAST of Lag0040625 vs. ExPASy Swiss-Prot
Match: Q42656 (Alpha-galactosidase OS=Coffea arabica OX=13443 PE=1 SV=1)

HSP 1 Score: 612.1 bits (1577), Expect = 4.9e-174
Identity = 289/366 (78.96%), Postives = 311/366 (84.97%), Query Frame = 0

Query: 55  DSTRRVLLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLDDC 114
           D TRR LL NGL LTPPMGWNSWNHF+CN++E LI+ETADAMVS GLAALGY+YINLDDC
Sbjct: 9   DYTRRSLLANGLGLTPPMGWNSWNHFRCNLDEKLIRETADAMVSKGLAALGYKYINLDDC 68

Query: 115 WAELNRDSKGNLVAKVSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEE 174
           WAELNRDS+GNLV K STFPSGIKALADYVH KGLKLGIYSDAG QTCSK MPGSLGHEE
Sbjct: 69  WAELNRDSQGNLVPKGSTFPSGIKALADYVHSKGLKLGIYSDAGTQTCSKTMPGSLGHEE 128

Query: 175 QDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWA 234
           QDAKTFASWG+DYLKYDNC N   SPKERYP M+KAL  SGR I FSLCEWG+EDPATWA
Sbjct: 129 QDAKTFASWGVDYLKYDNCNNNNISPKERYPIMSKALLNSGRSIFFSLCEWGEEDPATWA 188

Query: 235 VNVGNSWRTTSDIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMKTAEYRSH 294
             VGNSWRTT DI D+W+SMT+ AD NDKWASYA PGGWNDPDMLEVGNGGM T EYRSH
Sbjct: 189 KEVGNSWRTTGDIDDSWSSMTSRADMNDKWASYAGPGGWNDPDMLEVGNGGMTTTEYRSH 248

Query: 295 FSIWALAKAPLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVNKLGDLEVWAGP 354
           FSIWALAKAPLLIGCDIRSMD  T +LLSN EVIAVNQDKLGVQG KV   GDLEVWAGP
Sbjct: 249 FSIWALAKAPLLIGCDIRSMDGATFQLLSNAEVIAVNQDKLGVQGNKVKTYGDLEVWAGP 308

Query: 355 LSGKRVAVVLWNRGLWRANITASWSDIGLNPSTTVTARDLWEHSNQ-VVQHQLTAQVDSH 414
           LSGKRVAV LWNRG   A ITA WSD+GL  +  V ARDLW HS +  V+ Q++A VD+H
Sbjct: 309 LSGKRVAVALWNRGSSTATITAYWSDVGLPSTAVVNARDLWAHSTEKSVKGQISAAVDAH 368

Query: 415 DCKIYM 420
           D K+Y+
Sbjct: 369 DSKMYV 374

BLAST of Lag0040625 vs. ExPASy Swiss-Prot
Match: Q9FXT4 (Alpha-galactosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0493600 PE=1 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 7.1e-157
Identity = 257/370 (69.46%), Postives = 292/370 (78.92%), Query Frame = 0

Query: 50  AEFDSDSTRRVLLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYI 109
           A + S   RR    NGL  TP MGWNSWNHF C INE +I+ETADA+V++GLA LGY+Y+
Sbjct: 44  ARWRSRRARRRAFENGLGRTPQMGWNSWNHFYCGINEQIIRETADALVNTGLAKLGYQYV 103

Query: 110 NLDDCWAELNRDSKGNLVAKVSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGS 169
           N+DDCWAE +RDS+GN V    TFPSGIKALADYVH KGLKLGIYSDAG QTCS KMPGS
Sbjct: 104 NIDDCWAEYSRDSQGNFVPNRQTFPSGIKALADYVHAKGLKLGIYSDAGSQTCSNKMPGS 163

Query: 170 LGHEEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQED 229
           L HEEQD KTFASWG+DYLKYDNC + G S  ERY +M+ A++  G+ I FSLCEWG+E+
Sbjct: 164 LDHEEQDVKTFASWGVDYLKYDNCNDAGRSVMERYTRMSNAMKTYGKNIFFSLCEWGKEN 223

Query: 230 PATWAVNVGNSWRTTSDIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMKTA 289
           PATWA  +GNSWRTT DI DNW SMT+ AD+ND+WA+YA PGGWNDPDMLEVGNGGM  A
Sbjct: 224 PATWAGRMGNSWRTTGDIADNWGSMTSRADENDQWAAYAGPGGWNDPDMLEVGNGGMSEA 283

Query: 290 EYRSHFSIWALAKAPLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVNKLGDLE 349
           EYRSHFSIWALAKAPLLIGCD+RSM   T  +LSN EVIAVNQD LGVQGKKV     LE
Sbjct: 284 EYRSHFSIWALAKAPLLIGCDVRSMSQQTKNILSNSEVIAVNQDSLGVQGKKVQSDNGLE 343

Query: 350 VWAGPLSGKRVAVVLWNRGLWRANITASWSDIGLNPSTTVTARDLWEHSNQVVQHQLTAQ 409
           VWAGPLS  R AVVLWNR  ++A ITA WS+IGL  S  VTARDLW HS+   Q Q++A 
Sbjct: 344 VWAGPLSNNRKAVVLWNRQSYQATITAHWSNIGLAGSVAVTARDLWAHSSFAAQGQISAS 403

Query: 410 VDSHDCKIYM 420
           V  HDCK+Y+
Sbjct: 404 VAPHDCKMYV 413

BLAST of Lag0040625 vs. ExPASy Swiss-Prot
Match: Q9FT97 (Alpha-galactosidase 1 OS=Arabidopsis thaliana OX=3702 GN=AGAL1 PE=2 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 1.3e-150
Identity = 249/369 (67.48%), Postives = 293/369 (79.40%), Query Frame = 0

Query: 53  DSDSTRRVLLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLD 112
           DS+  RR LL NGL +TPPMGWNSWNHF CNI+E +IKETADA+V++GL+ LGY Y+N+D
Sbjct: 37  DSEILRRHLLTNGLGVTPPMGWNSWNHFSCNIDEKMIKETADALVTTGLSKLGYNYVNID 96

Query: 113 DCWAELNRDSKGNLVAKVSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGH 172
           DCWAE++RDSKG+LV K STFPSGIKA+ADYVH KGLKLGIYSDAG  TCSK MPGSLG+
Sbjct: 97  DCWAEISRDSKGSLVPKKSTFPSGIKAVADYVHSKGLKLGIYSDAGYFTCSKTMPGSLGY 156

Query: 173 EEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPAT 232
           EE DAKTFA WGIDYLKYDNC + G+ P  RYP MT+AL +SGRPI  SLCEWG   PA 
Sbjct: 157 EEHDAKTFAEWGIDYLKYDNCNSDGSKPTVRYPVMTRALMKSGRPIFHSLCEWGDMHPAL 216

Query: 233 WAVNVGNSWRTTSDIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMKTAEYR 292
           W   VGNSWRTT+DI+D W SM +IAD N+ +A +A+PGGWNDPDMLEVGNGGM   EY 
Sbjct: 217 WGSPVGNSWRTTNDIKDTWLSMISIADMNEVYAEHARPGGWNDPDMLEVGNGGMTKDEYI 276

Query: 293 SHFSIWALAKAPLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVNKLGDLEVWA 352
            HFSIWA++KAPLL+GCDIR+M   T+++++NKEVIA+NQD  GVQ KKV   GDLEVWA
Sbjct: 277 VHFSIWAISKAPLLLGCDIRNMTKETMEIVANKEVIAINQDPHGVQAKKVRMEGDLEVWA 336

Query: 353 GPLSGKRVAVVLWNRGLWRANITASWSDIGLNPSTTVTARDLWEHS--NQVVQHQLTAQV 412
           GPLSG RVA++L NRG  R +ITA W DI +  ++ V ARDLWEH    Q     LTA V
Sbjct: 337 GPLSGYRVALLLLNRGPSRTSITALWEDIEIPANSIVEARDLWEHQTLKQKFVGNLTATV 396

Query: 413 DSHDCKIYM 420
           DSH CK+Y+
Sbjct: 397 DSHACKLYV 405

BLAST of Lag0040625 vs. ExPASy TrEMBL
Match: A0A6J1FZL3 (Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111449362 PE=3 SV=1)

HSP 1 Score: 803.9 bits (2075), Expect = 3.2e-229
Identity = 383/412 (92.96%), Postives = 395/412 (95.87%), Query Frame = 0

Query: 8   LSSPASTMVLFYSVLFWTFLFGSNSDGGAVAGATRSRPLRFAAEFDSDSTRRVLLNNGLA 67
           +SSPAS M+  YSVLFWTFLF SN +GGA+AGATRSRPLRFAAEFD DS RRVLLNNGLA
Sbjct: 2   VSSPASIMLFLYSVLFWTFLFSSNGNGGAMAGATRSRPLRFAAEFDYDSPRRVLLNNGLA 61

Query: 68  LTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLDDCWAELNRDSKGNLV 127
           LTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGY+YINLDDCWAELNRD+KGNLV
Sbjct: 62  LTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYQYINLDDCWAELNRDAKGNLV 121

Query: 128 AKVSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGIDY 187
            K STFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGIDY
Sbjct: 122 PKSSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGIDY 181

Query: 188 LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI 247
           LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI
Sbjct: 182 LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI 241

Query: 248 QDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMKTAEYRSHFSIWALAKAPLLI 307
           QDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGM TAEYRSHFSIWALAKAPLLI
Sbjct: 242 QDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLI 301

Query: 308 GCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVNKLGDLEVWAGPLSGKRVAVVLWNR 367
           GCDIRSMDNITLKLLSNK VIAVNQDKLGVQGKKV KLGDLEVWAG LSGKRVAVVLWNR
Sbjct: 302 GCDIRSMDNITLKLLSNKLVIAVNQDKLGVQGKKVYKLGDLEVWAGALSGKRVAVVLWNR 361

Query: 368 GLWRANITASWSDIGLNPSTTVTARDLWEHSNQVVQHQLTAQVDSHDCKIYM 420
           G +RA ITASWS IGL+PSTTVTARDLWEHS+QVVQHQ+TAQVD HDCK+Y+
Sbjct: 362 GFYRAKITASWSAIGLSPSTTVTARDLWEHSSQVVQHQITAQVDPHDCKMYV 413

BLAST of Lag0040625 vs. ExPASy TrEMBL
Match: A0A6J1HTR9 (Alpha-galactosidase OS=Cucurbita maxima OX=3661 GN=LOC111466515 PE=3 SV=1)

HSP 1 Score: 803.5 bits (2074), Expect = 4.2e-229
Identity = 383/412 (92.96%), Postives = 394/412 (95.63%), Query Frame = 0

Query: 8   LSSPASTMVLFYSVLFWTFLFGSNSDGGAVAGATRSRPLRFAAEFDSDSTRRVLLNNGLA 67
           +SSPAS M+L YSVLFWTF F SN +GGA+AGATRSRPLRFA EFD DS RRVLLNNGLA
Sbjct: 2   VSSPASIMLLLYSVLFWTFFFSSNGNGGAMAGATRSRPLRFATEFDYDSPRRVLLNNGLA 61

Query: 68  LTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLDDCWAELNRDSKGNLV 127
           LTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGY+YINLDDCWAELNRD+KGNLV
Sbjct: 62  LTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYQYINLDDCWAELNRDAKGNLV 121

Query: 128 AKVSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWGIDY 187
            K STFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSL HEEQDAKTFASWGIDY
Sbjct: 122 PKSSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLDHEEQDAKTFASWGIDY 181

Query: 188 LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI 247
           LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI
Sbjct: 182 LKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTTSDI 241

Query: 248 QDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMKTAEYRSHFSIWALAKAPLLI 307
           QDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGM TAEYRSHFSIWALAKAPLLI
Sbjct: 242 QDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAPLLI 301

Query: 308 GCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVNKLGDLEVWAGPLSGKRVAVVLWNR 367
           GCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKV KLGDLEVWAG LSGKRVAVVLWNR
Sbjct: 302 GCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVYKLGDLEVWAGTLSGKRVAVVLWNR 361

Query: 368 GLWRANITASWSDIGLNPSTTVTARDLWEHSNQVVQHQLTAQVDSHDCKIYM 420
           G +RA ITASWS IGL+PSTTVTARDLWEHS+QVVQHQLTAQVD HDCK+Y+
Sbjct: 362 GFYRAKITASWSAIGLSPSTTVTARDLWEHSSQVVQHQLTAQVDPHDCKMYV 413

BLAST of Lag0040625 vs. ExPASy TrEMBL
Match: A0A1S3BEH7 (Alpha-galactosidase OS=Cucumis melo OX=3656 GN=LOC103488794 PE=3 SV=1)

HSP 1 Score: 787.7 bits (2033), Expect = 2.4e-224
Identity = 380/415 (91.57%), Postives = 393/415 (94.70%), Query Frame = 0

Query: 5   SLPLSSPASTMVLFYSVLFWTFLFGSNSDGGAVAGATRSRPLRFAAEFDSDSTRRVLLNN 64
           +L  SSP S MVL Y VL WTFL G N +G AVAGA+RS  LRFAAEFDS S+RRVLLNN
Sbjct: 2   ALSPSSP-SVMVLLYFVLSWTFLLGGNGNGVAVAGASRSTALRFAAEFDSVSSRRVLLNN 61

Query: 65  GLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLDDCWAELNRDSKG 124
           GLALTPPMGWNSWNHFQCN+NENLIKETADAMVSSGLAALGYEYINLDDCWAEL+RDSKG
Sbjct: 62  GLALTPPMGWNSWNHFQCNLNENLIKETADAMVSSGLAALGYEYINLDDCWAELDRDSKG 121

Query: 125 NLVAKVSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWG 184
           NLVAK STFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLG+EEQDAKTFASWG
Sbjct: 122 NLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGNEEQDAKTFASWG 181

Query: 185 IDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTT 244
           IDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTT
Sbjct: 182 IDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTT 241

Query: 245 SDIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMKTAEYRSHFSIWALAKAP 304
           SDIQDNW SMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGM TAEYRSHFSIWALAKAP
Sbjct: 242 SDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAP 301

Query: 305 LLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVNKLGDLEVWAGPLSGKRVAVVL 364
           LLIGCDIRSMDNIT+KLLSNKEVIAVNQDKLGVQGKKV K GDLEVWAGPLSGKRVAVVL
Sbjct: 302 LLIGCDIRSMDNITMKLLSNKEVIAVNQDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVVL 361

Query: 365 WNRGLWRANITASWSDIGLNPSTTVTARDLWEHSNQVVQHQLTAQVDSHDCKIYM 420
           WNRGLWRANITASWSDIGL  STTVTARDLW+HS+QVVQH LTAQVDSHDCK+++
Sbjct: 362 WNRGLWRANITASWSDIGLCSSTTVTARDLWQHSSQVVQHHLTAQVDSHDCKMFV 415

BLAST of Lag0040625 vs. ExPASy TrEMBL
Match: A0A5A7SVZ2 (Alpha-galactosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G003280 PE=3 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 5.9e-223
Identity = 380/416 (91.35%), Postives = 393/416 (94.47%), Query Frame = 0

Query: 5   SLPLSSPASTMVLFYSVLFWTFLFGSNSDGGAVAGATRSRPLRFAAEFDSDSTRRVLLNN 64
           +L  SSP S MVL Y VL WTFL G N +G AVAGA+RS  LRFAAEFDS S+RRVLLNN
Sbjct: 2   ALSPSSP-SVMVLLYFVLSWTFLLGGNGNGVAVAGASRSTALRFAAEFDSVSSRRVLLNN 61

Query: 65  GLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLDDCWAELNRDSKG 124
           GLALTPPMGWNSWNHFQCN+NENLIKETADAMVSSGLAALGYEYINLDDCWAEL+RDSKG
Sbjct: 62  GLALTPPMGWNSWNHFQCNLNENLIKETADAMVSSGLAALGYEYINLDDCWAELDRDSKG 121

Query: 125 NLVAKVSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTFASWG 184
           NLVAK STFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLG+EEQDAKTFASWG
Sbjct: 122 NLVAKASTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGNEEQDAKTFASWG 181

Query: 185 IDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTT 244
           IDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTT
Sbjct: 182 IDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNSWRTT 241

Query: 245 SDIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMKTAEYRSHFSIWALAKAP 304
           SDIQDNW SMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGM TAEYRSHFSIWALAKAP
Sbjct: 242 SDIQDNWISMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMTTAEYRSHFSIWALAKAP 301

Query: 305 LLIGCDIRSMDNITLKLLSNKEVIAVNQ-DKLGVQGKKVNKLGDLEVWAGPLSGKRVAVV 364
           LLIGCDIRSMDNIT+KLLSNKEVIAVNQ DKLGVQGKKV K GDLEVWAGPLSGKRVAVV
Sbjct: 302 LLIGCDIRSMDNITMKLLSNKEVIAVNQVDKLGVQGKKVYKYGDLEVWAGPLSGKRVAVV 361

Query: 365 LWNRGLWRANITASWSDIGLNPSTTVTARDLWEHSNQVVQHQLTAQVDSHDCKIYM 420
           LWNRGLWRANITASWSDIGL  STTVTARDLW+HS+QVVQH LTAQVDSHDCK+++
Sbjct: 362 LWNRGLWRANITASWSDIGLCSSTTVTARDLWQHSSQVVQHHLTAQVDSHDCKMFV 416

BLAST of Lag0040625 vs. ExPASy TrEMBL
Match: A0A6J1CCH0 (Alpha-galactosidase OS=Momordica charantia OX=3673 GN=LOC111010230 PE=3 SV=1)

HSP 1 Score: 773.5 bits (1996), Expect = 4.7e-220
Identity = 369/419 (88.07%), Postives = 387/419 (92.36%), Query Frame = 0

Query: 1   MAFPSLPLSSPASTMVLFYSVLFWTFLFGSNSDGGAVAGATRSRPLRFAAEFDSDSTRRV 60
           MAF  LPLSSP +++++FYS++F   L  S  DG AVAGA RSR L    +F +DSTRRV
Sbjct: 1   MAFSPLPLSSPPASIMVFYSLVFLILLLCSTGDGVAVAGAARSRRL----QFFTDSTRRV 60

Query: 61  LLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLDDCWAELNR 120
           LLNNGLALTPPMGWNSWNHFQCNINE LIKETADAMVSSGLAALGYEYINLDDCWAELNR
Sbjct: 61  LLNNGLALTPPMGWNSWNHFQCNINEKLIKETADAMVSSGLAALGYEYINLDDCWAELNR 120

Query: 121 DSKGNLVAKVSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTF 180
           D+KGNLVAK STFPSGIKALADYVH KGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTF
Sbjct: 121 DAKGNLVAKASTFPSGIKALADYVHSKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAKTF 180

Query: 181 ASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNS 240
           ASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNS
Sbjct: 181 ASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVGNS 240

Query: 241 WRTTSDIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMKTAEYRSHFSIWAL 300
           WRTTSDIQDNW SMT+IADQNDKW+SYA+PGGWNDPDMLEVGNGGM T EYRSHFSIWAL
Sbjct: 241 WRTTSDIQDNWTSMTSIADQNDKWSSYARPGGWNDPDMLEVGNGGMTTVEYRSHFSIWAL 300

Query: 301 AKAPLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVNKLGDLEVWAGPLSGKRV 360
           AKAPLLIGCDIRSMDNI  KLLSNKEVIAVNQDKLGVQGKKV   GDLEVWAGPLSGKRV
Sbjct: 301 AKAPLLIGCDIRSMDNIAFKLLSNKEVIAVNQDKLGVQGKKVKNFGDLEVWAGPLSGKRV 360

Query: 361 AVVLWNRGLWRANITASWSDIGLNPSTTVTARDLWEHSNQVVQHQLTAQVDSHDCKIYM 420
           AVVLWNRG WRANITA+WSDIGL+PSTTVTARDLWEHS  VVQHQLTA+VDSHDCK+Y+
Sbjct: 361 AVVLWNRGFWRANITANWSDIGLSPSTTVTARDLWEHSTHVVQHQLTAEVDSHDCKMYV 415

BLAST of Lag0040625 vs. TAIR 10
Match: AT5G08370.1 (alpha-galactosidase 2 )

HSP 1 Score: 622.9 bits (1605), Expect = 2.0e-178
Identity = 289/361 (80.06%), Postives = 318/361 (88.09%), Query Frame = 0

Query: 59  RVLLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLDDCWAEL 118
           R+L+NNGLAL+P MGWNSWNHFQCNINE LIK+TADAMVSSGL+A+GY+YIN+DDCW EL
Sbjct: 29  RMLMNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSAIGYKYINIDDCWGEL 88

Query: 119 NRDSKGNLVAKVSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAK 178
            RDS+G+LVAK STFPSGIKAL+DYVH KGLKLGIYSDAG  TCS+ MPGSLGHEEQDAK
Sbjct: 89  KRDSQGSLVAKASTFPSGIKALSDYVHSKGLKLGIYSDAGTLTCSQTMPGSLGHEEQDAK 148

Query: 179 TFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVG 238
           TFASWGIDYLKYDNCENTGTSP+ERYPKM+KAL  SGR I FSLCEWGQEDPATWA ++G
Sbjct: 149 TFASWGIDYLKYDNCENTGTSPRERYPKMSKALLNSGRSIFFSLCEWGQEDPATWAGDIG 208

Query: 239 NSWRTTSDIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMKTAEYRSHFSIW 298
           NSWRTT DIQDNW SMT IADQND+WASYA+PG WNDPDMLEVGNGGM   EY SHFSIW
Sbjct: 209 NSWRTTGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGGMTKEEYMSHFSIW 268

Query: 299 ALAKAPLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVNKLGDLEVWAGPLSGK 358
           ALAKAPLLIGCD+RSMD +T +LLSNKEVIAVNQDKLG+QGKKV K GDLEVWAGPLS K
Sbjct: 269 ALAKAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVKKEGDLEVWAGPLSKK 328

Query: 359 RVAVVLWNRGLWRANITASWSDIGLNPSTTVTARDLWEHSN-QVVQHQLTAQVDSHDCKI 418
           RVAV+LWNRG   ANITA W++IGLN S  V ARDLWEHS    V+ QL+A V+ H CK+
Sbjct: 329 RVAVILWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYSCVKKQLSALVEPHACKM 388

BLAST of Lag0040625 vs. TAIR 10
Match: AT5G08370.2 (alpha-galactosidase 2 )

HSP 1 Score: 622.9 bits (1605), Expect = 2.0e-178
Identity = 289/361 (80.06%), Postives = 318/361 (88.09%), Query Frame = 0

Query: 59  RVLLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLDDCWAEL 118
           R+L+NNGLAL+P MGWNSWNHFQCNINE LIK+TADAMVSSGL+A+GY+YIN+DDCW EL
Sbjct: 3   RMLMNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSAIGYKYINIDDCWGEL 62

Query: 119 NRDSKGNLVAKVSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGHEEQDAK 178
            RDS+G+LVAK STFPSGIKAL+DYVH KGLKLGIYSDAG  TCS+ MPGSLGHEEQDAK
Sbjct: 63  KRDSQGSLVAKASTFPSGIKALSDYVHSKGLKLGIYSDAGTLTCSQTMPGSLGHEEQDAK 122

Query: 179 TFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPATWAVNVG 238
           TFASWGIDYLKYDNCENTGTSP+ERYPKM+KAL  SGR I FSLCEWGQEDPATWA ++G
Sbjct: 123 TFASWGIDYLKYDNCENTGTSPRERYPKMSKALLNSGRSIFFSLCEWGQEDPATWAGDIG 182

Query: 239 NSWRTTSDIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMKTAEYRSHFSIW 298
           NSWRTT DIQDNW SMT IADQND+WASYA+PG WNDPDMLEVGNGGM   EY SHFSIW
Sbjct: 183 NSWRTTGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGGMTKEEYMSHFSIW 242

Query: 299 ALAKAPLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVNKLGDLEVWAGPLSGK 358
           ALAKAPLLIGCD+RSMD +T +LLSNKEVIAVNQDKLG+QGKKV K GDLEVWAGPLS K
Sbjct: 243 ALAKAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVKKEGDLEVWAGPLSKK 302

Query: 359 RVAVVLWNRGLWRANITASWSDIGLNPSTTVTARDLWEHSN-QVVQHQLTAQVDSHDCKI 418
           RVAV+LWNRG   ANITA W++IGLN S  V ARDLWEHS    V+ QL+A V+ H CK+
Sbjct: 303 RVAVILWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYSCVKKQLSALVEPHACKM 362

BLAST of Lag0040625 vs. TAIR 10
Match: AT5G08380.1 (alpha-galactosidase 1 )

HSP 1 Score: 534.3 bits (1375), Expect = 9.2e-152
Identity = 249/369 (67.48%), Postives = 293/369 (79.40%), Query Frame = 0

Query: 53  DSDSTRRVLLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYEYINLD 112
           DS+  RR LL NGL +TPPMGWNSWNHF CNI+E +IKETADA+V++GL+ LGY Y+N+D
Sbjct: 37  DSEILRRHLLTNGLGVTPPMGWNSWNHFSCNIDEKMIKETADALVTTGLSKLGYNYVNID 96

Query: 113 DCWAELNRDSKGNLVAKVSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMPGSLGH 172
           DCWAE++RDSKG+LV K STFPSGIKA+ADYVH KGLKLGIYSDAG  TCSK MPGSLG+
Sbjct: 97  DCWAEISRDSKGSLVPKKSTFPSGIKAVADYVHSKGLKLGIYSDAGYFTCSKTMPGSLGY 156

Query: 173 EEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQEDPAT 232
           EE DAKTFA WGIDYLKYDNC + G+ P  RYP MT+AL +SGRPI  SLCEWG   PA 
Sbjct: 157 EEHDAKTFAEWGIDYLKYDNCNSDGSKPTVRYPVMTRALMKSGRPIFHSLCEWGDMHPAL 216

Query: 233 WAVNVGNSWRTTSDIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMKTAEYR 292
           W   VGNSWRTT+DI+D W SM +IAD N+ +A +A+PGGWNDPDMLEVGNGGM   EY 
Sbjct: 217 WGSPVGNSWRTTNDIKDTWLSMISIADMNEVYAEHARPGGWNDPDMLEVGNGGMTKDEYI 276

Query: 293 SHFSIWALAKAPLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVNKLGDLEVWA 352
            HFSIWA++KAPLL+GCDIR+M   T+++++NKEVIA+NQD  GVQ KKV   GDLEVWA
Sbjct: 277 VHFSIWAISKAPLLLGCDIRNMTKETMEIVANKEVIAINQDPHGVQAKKVRMEGDLEVWA 336

Query: 353 GPLSGKRVAVVLWNRGLWRANITASWSDIGLNPSTTVTARDLWEHS--NQVVQHQLTAQV 412
           GPLSG RVA++L NRG  R +ITA W DI +  ++ V ARDLWEH    Q     LTA V
Sbjct: 337 GPLSGYRVALLLLNRGPSRTSITALWEDIEIPANSIVEARDLWEHQTLKQKFVGNLTATV 396

Query: 413 DSHDCKIYM 420
           DSH CK+Y+
Sbjct: 397 DSHACKLYV 405

BLAST of Lag0040625 vs. TAIR 10
Match: AT3G56310.1 (Melibiase family protein )

HSP 1 Score: 507.7 bits (1306), Expect = 9.2e-144
Identity = 242/377 (64.19%), Postives = 282/377 (74.80%), Query Frame = 0

Query: 48  FAAEFDSDSTRRVLLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYE 107
           F + +D+    R+ LNNGLA TP MGWNSWN F CNINE +IKETADA+VSSGLA LGY 
Sbjct: 51  FNSIYDTSMYGRLQLNNGLARTPQMGWNSWNFFACNINETVIKETADALVSSGLADLGYI 110

Query: 108 YINLDDCWAELNRDSKGNLVAKVSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMP 167
           ++N+DDCW+ L RDS+G LV    TFPSGIK LADYVH KGLKLGIYSDAG+ TC +  P
Sbjct: 111 HVNIDDCWSNLLRDSEGQLVPHPETFPSGIKLLADYVHSKGLKLGIYSDAGVFTC-EVHP 170

Query: 168 GSLGHEEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQ 227
           GSL HE  DA  FASWG+DYLKYDNC N G  P ERYP M  AL  +GR I +SLCEWG 
Sbjct: 171 GSLFHEVDDADIFASWGVDYLKYDNCFNLGIKPIERYPPMRDALNATGRSIFYSLCEWGV 230

Query: 228 EDPATWAVNVGNSWRTTSDIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMK 287
           +DPA WA  VGNSWRTT DI D W SMTTIAD N+KWA+YA PGGWNDPDMLE+GNGGM 
Sbjct: 231 DDPALWAKEVGNSWRTTDDINDTWASMTTIADLNNKWAAYAGPGGWNDPDMLEIGNGGMT 290

Query: 288 TAEYRSHFSIWALAKAPLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVNKLGD 347
             EYR HFSIWAL KAPLLIGCD+R+M   TL++LSNKE+IAVNQD LGVQG+K+   G+
Sbjct: 291 YEEYRGHFSIWALMKAPLLIGCDVRNMTAETLEILSNKEIIAVNQDPLGVQGRKIQANGE 350

Query: 348 ---LEVWAGPLSGKRVAVVLWNRGLWRANITASWSDIGLNPSTTVTARDLWEHSN--QVV 407
               +VW+GPLSG R+ V LWNR    A ITASW  IGL  + +V+ RDLW+H +  +  
Sbjct: 351 NDCQQVWSGPLSGDRMVVALWNRCSEPATITASWDMIGLESTISVSVRDLWQHKDVTENT 410

Query: 408 QHQLTAQVDSHDCKIYM 420
                AQVD+HDC +Y+
Sbjct: 411 SGSFEAQVDAHDCHMYV 426

BLAST of Lag0040625 vs. TAIR 10
Match: AT3G56310.2 (Melibiase family protein )

HSP 1 Score: 461.1 bits (1185), Expect = 9.8e-130
Identity = 227/377 (60.21%), Postives = 265/377 (70.29%), Query Frame = 0

Query: 48  FAAEFDSDSTRRVLLNNGLALTPPMGWNSWNHFQCNINENLIKETADAMVSSGLAALGYE 107
           F + +D+    R+ LNNGLA TP MGWNSWN F CNINE +IKETADA+VSSGLA LGY 
Sbjct: 51  FNSIYDTSMYGRLQLNNGLARTPQMGWNSWNFFACNINETVIKETADALVSSGLADLGYI 110

Query: 108 YINLDDCWAELNRDSKGNLVAKVSTFPSGIKALADYVHRKGLKLGIYSDAGIQTCSKKMP 167
           ++N+                        GIK LADYVH KGLKLGIYSDAG+ TC +  P
Sbjct: 111 HVNI------------------------GIKLLADYVHSKGLKLGIYSDAGVFTC-EVHP 170

Query: 168 GSLGHEEQDAKTFASWGIDYLKYDNCENTGTSPKERYPKMTKALQQSGRPILFSLCEWGQ 227
           GSL HE  DA  FASWG+DYLKYDNC N G  P ERYP M  AL  +GR I +SLCEWG 
Sbjct: 171 GSLFHEVDDADIFASWGVDYLKYDNCFNLGIKPIERYPPMRDALNATGRSIFYSLCEWGV 230

Query: 228 EDPATWAVNVGNSWRTTSDIQDNWNSMTTIADQNDKWASYAKPGGWNDPDMLEVGNGGMK 287
           +DPA WA  VGNSWRTT DI D W SMTTIAD N+KWA+YA PGGWNDPDMLE+GNGGM 
Sbjct: 231 DDPALWAKEVGNSWRTTDDINDTWASMTTIADLNNKWAAYAGPGGWNDPDMLEIGNGGMT 290

Query: 288 TAEYRSHFSIWALAKAPLLIGCDIRSMDNITLKLLSNKEVIAVNQDKLGVQGKKVNKLGD 347
             EYR HFSIWAL KAPLLIGCD+R+M   TL++LSNKE+IAVNQD LGVQG+K+   G+
Sbjct: 291 YEEYRGHFSIWALMKAPLLIGCDVRNMTAETLEILSNKEIIAVNQDPLGVQGRKIQANGE 350

Query: 348 ---LEVWAGPLSGKRVAVVLWNRGLWRANITASWSDIGLNPSTTVTARDLWEHSN--QVV 407
               +VW+GPLSG R+ V LWNR    A ITASW  IGL  + +V+ RDLW+H +  +  
Sbjct: 351 NDCQQVWSGPLSGDRMVVALWNRCSEPATITASWDMIGLESTISVSVRDLWQHKDVTENT 402

Query: 408 QHQLTAQVDSHDCKIYM 420
                AQVD+HDC +Y+
Sbjct: 411 SGSFEAQVDAHDCHMYV 402

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023541567.12.3e-22993.20alpha-galactosidase-like [Cucurbita pepo subsp. pepo][more]
XP_022944991.16.6e-22992.96alpha-galactosidase-like [Cucurbita moschata][more]
XP_022966963.18.7e-22992.96alpha-galactosidase-like [Cucurbita maxima][more]
KAG6573784.16.2e-22792.70Alpha-galactosidase 2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_038892679.12.4e-22691.65alpha-galactosidase-like isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q8RX862.8e-17780.06Alpha-galactosidase 2 OS=Arabidopsis thaliana OX=3702 GN=AGAL2 PE=1 SV=1[more]
P147494.4e-17573.58Alpha-galactosidase OS=Cyamopsis tetragonoloba OX=3832 PE=1 SV=1[more]
Q426564.9e-17478.96Alpha-galactosidase OS=Coffea arabica OX=13443 PE=1 SV=1[more]
Q9FXT47.1e-15769.46Alpha-galactosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0493600 PE=... [more]
Q9FT971.3e-15067.48Alpha-galactosidase 1 OS=Arabidopsis thaliana OX=3702 GN=AGAL1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FZL33.2e-22992.96Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111449362 PE=3 SV=1[more]
A0A6J1HTR94.2e-22992.96Alpha-galactosidase OS=Cucurbita maxima OX=3661 GN=LOC111466515 PE=3 SV=1[more]
A0A1S3BEH72.4e-22491.57Alpha-galactosidase OS=Cucumis melo OX=3656 GN=LOC103488794 PE=3 SV=1[more]
A0A5A7SVZ25.9e-22391.35Alpha-galactosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G0... [more]
A0A6J1CCH04.7e-22088.07Alpha-galactosidase OS=Momordica charantia OX=3673 GN=LOC111010230 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G08370.12.0e-17880.06alpha-galactosidase 2 [more]
AT5G08370.22.0e-17880.06alpha-galactosidase 2 [more]
AT5G08380.19.2e-15267.48alpha-galactosidase 1 [more]
AT3G56310.19.2e-14464.19Melibiase family protein [more]
AT3G56310.29.8e-13060.21Melibiase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002241Glycoside hydrolase, family 27PRINTSPR00740GLHYDRLASE27coord: 176..193
score: 76.39
coord: 141..162
score: 62.5
coord: 289..310
score: 55.91
coord: 203..221
score: 52.37
coord: 268..287
score: 65.75
coord: 64..83
score: 66.5
coord: 100..115
score: 69.06
IPR002241Glycoside hydrolase, family 27PFAMPF16499Melibiase_2coord: 69..333
e-value: 1.1E-81
score: 274.1
IPR002241Glycoside hydrolase, family 27PANTHERPTHR11452ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASEcoord: 52..419
IPR002241Glycoside hydrolase, family 27CDDcd14792GH27coord: 70..333
e-value: 8.64911E-162
score: 454.321
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 62..335
e-value: 1.0E-139
score: 466.7
IPR013780Glycosyl hydrolase, all-betaGENE3D2.60.40.1180coord: 336..421
e-value: 7.1E-28
score: 98.4
IPR041233Alpha galactosidase, C-terminal beta sandwich domainPFAMPF17801Melibiase_Ccoord: 346..419
e-value: 2.3E-19
score: 69.2
NoneNo IPR availablePANTHERPTHR11452:SF33ALPHA-GALACTOSIDASE 2coord: 52..419
NoneNo IPR availableSUPERFAMILY51011Glycosyl hydrolase domaincoord: 328..420
IPR000111Glycoside hydrolase family 27/36, conserved sitePROSITEPS00512ALPHA_GALACTOSIDASEcoord: 105..121
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 60..333

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0040625.1Lag0040625.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0009965 leaf morphogenesis
biological_process GO:0009911 positive regulation of flower development
biological_process GO:0009620 response to fungus
cellular_component GO:0048046 apoplast
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009505 plant-type cell wall
molecular_function GO:0052692 raffinose alpha-galactosidase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds