Lag0040609 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0040609 vs. NCBI nr
Match: XP_022138947.1 (mediator of RNA polymerase II transcription subunit 15a-like isoform X1 [Momordica charantia])

HSP 1 Score: 553.5 bits (1425), Expect = 1.5e-153
Identity = 276/360 (76.67%), Postives = 305/360 (84.72%), Query Frame = 0

Query: 50  MLPGLPGNGRADYEA-AWDDFFCPQRGNMSHQMVNDAVHQEKAFIISTPGMSPSTLLEEV 109
           M+PGLP NGRAD E  AWDDF   QRG +SHQ+VNDAV +EK FIISTPGMSPS LLEEV
Sbjct: 1   MVPGLPENGRADCEEDAWDDFLFSQRGKVSHQIVNDAVQEEKPFIISTPGMSPSALLEEV 60

Query: 110 TESDRENAICCVSPLQRLIKVVNVMSPKALSASVSDISSLVCMTDRVSSSAPLNKSKAAI 169
           +ESDR+N IC V+PLQRL+K+VNVMSPKALSAS+SDI S+VCMTDRV+SSAPLNKSKA I
Sbjct: 61  SESDRDNDICSVTPLQRLLKMVNVMSPKALSASISDIDSIVCMTDRVASSAPLNKSKAEI 120

Query: 170 GGDLVGAVESRLRRNCSRFGGTIGSRRTRNYRSARMYASSRRLMDCEGFDLNSIFTQTSR 229
             DLVG V SRLR+ CS FGG+ GSRRTR+ R+ RMY SSRR+M CE +DLNS FT+ SR
Sbjct: 121 SEDLVGTVTSRLRKRCSIFGGSTGSRRTRHCRNTRMYTSSRRMMYCESYDLNSTFTERSR 180

Query: 230 VETPEHLHWHGFSLPVKQSLLEEIRRINEQLIDTVVSICDKDMRTTDTTATIDRSEGITV 289
           +ETPEH HW GFSLP KQ LLEEIR+INEQLIDTVVS+CDKDM TTD  AT DRSEGI V
Sbjct: 181 IETPEHSHWRGFSLPFKQYLLEEIRKINEQLIDTVVSVCDKDMITTDNAATNDRSEGIIV 240

Query: 290 ECSFVAMHTNSNLISQQTPVLQLAVHPLKLLVPKGYPHISPIFKKFHVEDCPTDCEDLSL 349
           ECSF AM  +SN ISQQ   LQL VHPLKLLVPKGYPH SPIF K  +E CPTDCE+LSL
Sbjct: 241 ECSFAAMRISSNKISQQASALQLTVHPLKLLVPKGYPHCSPIFNKLKLEACPTDCEELSL 300

Query: 350 KAREKFNMSVRSLPHPFTISEVARTWDVCAREVVSEYAERNGGGSFSSKYGSWEDCLHAA 409
           K REKFNM VRSLPHPF+ISEVARTWD CAREVVSE+A+RNGGGSF+SKYGSWEDCLH A
Sbjct: 301 KTREKFNMCVRSLPHPFSISEVARTWDACAREVVSEFAQRNGGGSFTSKYGSWEDCLHHA 360

BLAST of Lag0040609 vs. NCBI nr
Match: KAG6601093.1 (Mediator of RNA polymerase II transcription subunit 15a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 525.8 bits (1353), Expect = 3.4e-145
Identity = 276/373 (73.99%), Postives = 307/373 (82.31%), Query Frame = 0

Query: 39  FGKLAYRKRKTMLPGLPGNGRADYEAAWDDFFCPQRGNMSHQMVNDAVHQEKAFIISTPG 98
           F KLAY K + MLPGL  NG  D+E A ++F  P        +VNDA   EKAFI+STPG
Sbjct: 65  FWKLAYWKWRIMLPGLLENGSVDFEKALENFLYP-------PIVNDAAQAEKAFIVSTPG 124

Query: 99  MSPSTLLEEVTESDRENAICCVSPLQRLIKVVNVMSPKALSASVSDISSLVCMTDRVSSS 158
           MSPS+LLEEVT+SDR+N  CCV+PLQRLIK+VNVMSPKALSASVSDI S+VCMTDRVSSS
Sbjct: 125 MSPSSLLEEVTDSDRDNDKCCVTPLQRLIKMVNVMSPKALSASVSDIDSIVCMTDRVSSS 184

Query: 159 APLNKSKAAIGGDLVGAVESRLRRNCSRFGGTIGSRRTRNYRSARMYASSRRLMDCEGFD 218
           APLNKSK A+G DL G V SRLRR CSRFGGTIGSRRTR+YR          LMDCEGFD
Sbjct: 185 APLNKSKGAVGMDLAGTVASRLRRKCSRFGGTIGSRRTRHYRC---------LMDCEGFD 244

Query: 219 LNSIFTQTSR-VETPEHLHWHGFSLPVKQSLLEEIRRINEQLIDTVVSICDKDMRTTDTT 278
           L+S+FTQ SR +ETP+H +WHGFSLPVKQSLLEEIRRINEQLI T VSICDKDM TTD  
Sbjct: 245 LSSLFTQRSRIIETPKHSNWHGFSLPVKQSLLEEIRRINEQLIYTEVSICDKDMVTTDPG 304

Query: 279 ATIDRSEGITVECSFVAMHTNSNLISQQTPVLQLAVHPLKLLVPKGYPHISPIFKKFHVE 338
           ATI RSEGI VECSF A+ T S+LIS+QT  +QLA+HPLKLLVP GYPHISPIFK   +E
Sbjct: 305 ATIGRSEGILVECSFGAVCTLSHLISRQTSAIQLAMHPLKLLVPIGYPHISPIFK---ME 364

Query: 339 DCPTDCEDLSLKAREKFNMSVRSLPHPFTISEVARTWDVCAREVVSEYAERNGGGSFSSK 398
            CPT CEDLSL+AREKFNMSVRSL HPF+ISEVARTWD+CAR+V+SEYA +NGGGSFSSK
Sbjct: 365 ACPTGCEDLSLQAREKFNMSVRSLAHPFSISEVARTWDMCARDVISEYARQNGGGSFSSK 418

Query: 399 YGSWEDCLHAAWS 411
           YGSWEDCLH AWS
Sbjct: 425 YGSWEDCLHTAWS 418

BLAST of Lag0040609 vs. NCBI nr
Match: XP_022984739.1 (mediator of RNA polymerase II transcription subunit 15a-like [Cucurbita maxima])

HSP 1 Score: 525.8 bits (1353), Expect = 3.4e-145
Identity = 275/373 (73.73%), Postives = 306/373 (82.04%), Query Frame = 0

Query: 39  FGKLAYRKRKTMLPGLPGNGRADYEAAWDDFFCPQRGNMSHQMVNDAVHQEKAFIISTPG 98
           F KLAYRK + MLPGL  NG  D+E A +DF  P        +VNDA   EKAFI+STPG
Sbjct: 65  FWKLAYRKWRIMLPGLLENGSVDFEKALEDFLYP-------PIVNDAAQAEKAFIVSTPG 124

Query: 99  MSPSTLLEEVTESDRENAICCVSPLQRLIKVVNVMSPKALSASVSDISSLVCMTDRVSSS 158
           MSPS+LLEEVT+SDR+N  CCV+PLQRLIK+VNVMSPKALSASVSDI S+VCMTDRVSSS
Sbjct: 125 MSPSSLLEEVTDSDRDNDKCCVTPLQRLIKMVNVMSPKALSASVSDIDSIVCMTDRVSSS 184

Query: 159 APLNKSKAAIGGDLVGAVESRLRRNCSRFGGTIGSRRTRNYRSARMYASSRRLMDCEGFD 218
           APLNKSK A+G DL G V SRLRR CSRFGGTIGSRRTR+YR          LMDCEGFD
Sbjct: 185 APLNKSKGAVGMDLAGTVASRLRRKCSRFGGTIGSRRTRHYRC---------LMDCEGFD 244

Query: 219 LNSIFTQTSR-VETPEHLHWHGFSLPVKQSLLEEIRRINEQLIDTVVSICDKDMRTTDTT 278
           L+S+FTQ SR +ETP+H +WHGFSLPVKQSLLEEIRRINEQLI T VSICDKDM TTD  
Sbjct: 245 LSSLFTQRSRIIETPKHSNWHGFSLPVKQSLLEEIRRINEQLIYTEVSICDKDMVTTDPA 304

Query: 279 ATIDRSEGITVECSFVAMHTNSNLISQQTPVLQLAVHPLKLLVPKGYPHISPIFKKFHVE 338
           ATI +SEGI VECSF A+ T S+LIS QT  +QLA+ PLKLLVP GYPHISPIFK   +E
Sbjct: 305 ATIGKSEGILVECSFGAVCTLSHLISHQTSAIQLAMQPLKLLVPIGYPHISPIFK---ME 364

Query: 339 DCPTDCEDLSLKAREKFNMSVRSLPHPFTISEVARTWDVCAREVVSEYAERNGGGSFSSK 398
            CPT CEDLSL+AREKFNMSVRSL HPF+ISE+ARTWD+CAR+V+SEYA +NGGGSFSSK
Sbjct: 365 ACPTGCEDLSLQAREKFNMSVRSLAHPFSISEMARTWDMCARDVISEYARQNGGGSFSSK 418

Query: 399 YGSWEDCLHAAWS 411
           YGSWEDCLH AWS
Sbjct: 425 YGSWEDCLHTAWS 418

BLAST of Lag0040609 vs. NCBI nr
Match: XP_022956517.1 (mediator of RNA polymerase II transcription subunit 15a-like [Cucurbita moschata] >XP_022956518.1 mediator of RNA polymerase II transcription subunit 15a-like [Cucurbita moschata])

HSP 1 Score: 525.4 bits (1352), Expect = 4.4e-145
Identity = 276/373 (73.99%), Postives = 306/373 (82.04%), Query Frame = 0

Query: 39  FGKLAYRKRKTMLPGLPGNGRADYEAAWDDFFCPQRGNMSHQMVNDAVHQEKAFIISTPG 98
           F KLAY K + MLPGL  NG  D+E A ++F  P        +VNDA   EKAFI+STPG
Sbjct: 65  FWKLAYWKWRIMLPGLLENGSVDFEKALENFLYP-------PIVNDAAQAEKAFIVSTPG 124

Query: 99  MSPSTLLEEVTESDRENAICCVSPLQRLIKVVNVMSPKALSASVSDISSLVCMTDRVSSS 158
           MSPS+LLEEVT+SDR+N  CCV+PLQRLIK+VNVMSPKALSASVSDI S+VCMTDRVSSS
Sbjct: 125 MSPSSLLEEVTDSDRDNDKCCVTPLQRLIKMVNVMSPKALSASVSDIDSIVCMTDRVSSS 184

Query: 159 APLNKSKAAIGGDLVGAVESRLRRNCSRFGGTIGSRRTRNYRSARMYASSRRLMDCEGFD 218
           APLNKSK A+G DL G V SRLRR CSRFGGTIGSRRTR+YR          LMDCEGFD
Sbjct: 185 APLNKSKGAVGMDLAGTVASRLRRKCSRFGGTIGSRRTRHYRC---------LMDCEGFD 244

Query: 219 LNSIFTQTSR-VETPEHLHWHGFSLPVKQSLLEEIRRINEQLIDTVVSICDKDMRTTDTT 278
           L+S+FTQ SR +ETP+H +WHGFSLPVKQSLLEEIRRINEQLI T VSICDKDM TTD  
Sbjct: 245 LSSLFTQRSRIIETPKHSNWHGFSLPVKQSLLEEIRRINEQLIYTEVSICDKDMVTTDPG 304

Query: 279 ATIDRSEGITVECSFVAMHTNSNLISQQTPVLQLAVHPLKLLVPKGYPHISPIFKKFHVE 338
           ATI RSEGI VECSF A+ T S+LIS QT  +QLA+HPLKLLVP GYPHISPIFK   +E
Sbjct: 305 ATIGRSEGILVECSFGAVCTLSHLISHQTSAIQLAMHPLKLLVPIGYPHISPIFK---ME 364

Query: 339 DCPTDCEDLSLKAREKFNMSVRSLPHPFTISEVARTWDVCAREVVSEYAERNGGGSFSSK 398
            CPT CEDLSL+AREKFNMSVRSL HPF+ISEVARTWD+CAR+V+SEYA +NGGGSFSSK
Sbjct: 365 ACPTGCEDLSLQAREKFNMSVRSLAHPFSISEVARTWDMCARDVISEYARQNGGGSFSSK 418

Query: 399 YGSWEDCLHAAWS 411
           YGSWEDCLH AWS
Sbjct: 425 YGSWEDCLHTAWS 418

BLAST of Lag0040609 vs. NCBI nr
Match: XP_023526658.1 (mediator of RNA polymerase II transcription subunit 15a-like [Cucurbita pepo subsp. pepo] >XP_023526666.1 mediator of RNA polymerase II transcription subunit 15a-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 524.6 bits (1350), Expect = 7.5e-145
Identity = 275/373 (73.73%), Postives = 306/373 (82.04%), Query Frame = 0

Query: 39  FGKLAYRKRKTMLPGLPGNGRADYEAAWDDFFCPQRGNMSHQMVNDAVHQEKAFIISTPG 98
           F KLAYRK + MLPGL  NG  D+E A ++F  P        +VNDA   EKAFI+STPG
Sbjct: 65  FWKLAYRKWRIMLPGLLENGSVDFEKALENFLYP-------PIVNDAAQAEKAFIVSTPG 124

Query: 99  MSPSTLLEEVTESDRENAICCVSPLQRLIKVVNVMSPKALSASVSDISSLVCMTDRVSSS 158
           MSPS+LLEEVT+SDR+N  CCV+PLQRLIK+VNVMSPKALSASVSDI S+VCMTDRV SS
Sbjct: 125 MSPSSLLEEVTDSDRDNDKCCVTPLQRLIKMVNVMSPKALSASVSDIDSIVCMTDRVPSS 184

Query: 159 APLNKSKAAIGGDLVGAVESRLRRNCSRFGGTIGSRRTRNYRSARMYASSRRLMDCEGFD 218
           APLNKSK A+G DL G V SRLRR CSRFGGT GSRRTR+YR          LMDCEGFD
Sbjct: 185 APLNKSKGAVGMDLAGTVASRLRRKCSRFGGTNGSRRTRHYRC---------LMDCEGFD 244

Query: 219 LNSIFTQTSRV-ETPEHLHWHGFSLPVKQSLLEEIRRINEQLIDTVVSICDKDMRTTDTT 278
           L+S+FTQ SR+ ETP+H +WHGFSLPVKQSLLEEIRRINEQLI T VSICDKDM TTD+ 
Sbjct: 245 LSSLFTQRSRITETPKHSNWHGFSLPVKQSLLEEIRRINEQLIYTEVSICDKDMVTTDSA 304

Query: 279 ATIDRSEGITVECSFVAMHTNSNLISQQTPVLQLAVHPLKLLVPKGYPHISPIFKKFHVE 338
           ATI RSEGI VECSF A+ T S+LIS QT  +QLA+HPLKLLVP GYPHISPIFK   +E
Sbjct: 305 ATIGRSEGILVECSFGAVCTLSHLISPQTSAVQLAMHPLKLLVPIGYPHISPIFK---ME 364

Query: 339 DCPTDCEDLSLKAREKFNMSVRSLPHPFTISEVARTWDVCAREVVSEYAERNGGGSFSSK 398
            CPT CEDLSL+AREKFNMSVRSL HPF+ISEVARTWD+CAR+V+SEYA +NGGGSFSSK
Sbjct: 365 ACPTGCEDLSLQAREKFNMSVRSLAHPFSISEVARTWDMCARDVISEYARQNGGGSFSSK 418

Query: 399 YGSWEDCLHAAWS 411
           YGSWEDCLH AWS
Sbjct: 425 YGSWEDCLHTAWS 418

BLAST of Lag0040609 vs. ExPASy Swiss-Prot
Match: F4I171 (Mediator of RNA polymerase II transcription subunit 15a OS=Arabidopsis thaliana OX=3702 GN=MED15A PE=1 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 3.4e-47
Identity = 130/349 (37.25%), Postives = 196/349 (56.16%), Query Frame = 0

Query: 75   GNMSHQMVNDAVHQEKAFIISTPGMSPSTLLEEVTESDRE--NAICCVS--------PLQ 134
            GN++ Q         ++  I TPG+S S LL+E T  D    N+    S        P++
Sbjct: 989  GNIARQQATGMQGVVQSLAIGTPGISASPLLQEFTSPDGNILNSSTITSGKPSATELPIE 1048

Query: 135  RLIKVVNVMSPKALSASVSDISSLVCMTDRVSSSAPLNKSKAAIGGDLVGAVESRLR-RN 194
            RLI+ V  +SP+ALS++VSDI S+V M DR++ SAP N S+A++G DLV   + RL+ RN
Sbjct: 1049 RLIRAVKSISPQALSSAVSDIGSVVSMVDRIAGSAPGNGSRASVGEDLVAMTKCRLQARN 1108

Query: 195  CSRFGGTIGSRRTRNYRSARMYASSRRLMDCEGFDLNSIF-TQTSRVETPEHLHWHGFSL 254
                 G + +++ + + +A M  S   L    G +      ++TS +E+           
Sbjct: 1109 FMTQEGMMATKKMKRHTTA-MPLSVASLGGSVGDNYKQFAGSETSDLESTATSDGKKART 1168

Query: 255  PVKQSLLEEIRRINEQLIDTVVSICDKDMRTTDTTATIDR--SEGITVECSFVAMHTNSN 314
              + +LLEEI+ IN++LIDTVV I D +     +   I     EG TV  SF+A+  +  
Sbjct: 1169 ETEHALLEEIKEINQRLIDTVVEISDDEDAADPSEVAISSIGCEGTTVRFSFIAVSLSPA 1228

Query: 315  LISQQTPVLQLAVHPLKLLVPKGYPHISP-IFKKFHVEDCPTDCEDLSLKAREKFNMSVR 374
            L +  +      + PL+LLVP  YP+ SP +  K  VE    + EDLS KA  +FN+ +R
Sbjct: 1229 LKAHLSSTQMSPIQPLRLLVPCSYPNGSPSLLDKLPVETSKEN-EDLSSKAMARFNILLR 1288

Query: 375  SLPHPFTISEVARTWDVCAREVVSEYAERNGGGSFSSKYGSWEDCLHAA 409
            SL  P ++ ++A+TWD CAR V+ EYA++ GGG+FSSKYG+WE  + A+
Sbjct: 1289 SLSQPMSLKDIAKTWDACARAVICEYAQQFGGGTFSSKYGTWEKYVAAS 1335

BLAST of Lag0040609 vs. ExPASy Swiss-Prot
Match: Q9SHV7 (Probable mediator of RNA polymerase II transcription subunit 15c OS=Arabidopsis thaliana OX=3702 GN=MED15C PE=3 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 1.3e-30
Identity = 100/301 (33.22%), Postives = 158/301 (52.49%), Query Frame = 0

Query: 122 PLQRLIKVVNVMSPKALSASVSDISSLVCMTDRVSSSAPLN-KSKAAIGGDLVGAVESRL 181
           P+ RLIK     SPK+L+ SVS+ISS++ M D +  S P +  S+A +G DL        
Sbjct: 659 PIDRLIKAFQAASPKSLAESVSEISSVISMVDMIGGSFPSSGGSRAGLGEDL-------- 718

Query: 182 RRNCSRFGGTIGSRRTRNYRSARMYASSRRL---MDCEGFDLNS---IFTQTSRVETPEH 241
                       S RTRN+ +      S+R+   ++    D++S    + Q S +E+ E 
Sbjct: 719 ------------SERTRNFTTHEETNLSKRMKRSINIVPPDMSSQIDSYEQLSSLES-EV 778

Query: 242 LHWHGFSLPVKQ-----SLLEEIRRINEQLIDTVVSICDKDMRTTDTTATIDRSEGITVE 301
           +      L V       +LL+EI+  N +L++TVV ICD+D   T  T T      + + 
Sbjct: 779 VSTTSSGLKVNNIAPGYALLQEIKETNGRLVETVVEICDEDSLGTIVTCTY---APVALS 838

Query: 302 CSFVAMHTNSNLISQQTP-VLQLAVHPLKLLVPKGYPHISPI-FKKFHVEDCPTDCEDLS 361
            +F   + +  +I   +  ++Q  + PL+LL P  YP+ SPI  ++   +      EDLS
Sbjct: 839 ATFKDHYKSGKIIFYVSKCLMQAQIQPLRLLFPMDYPYSSPIVLEEISFDTSVHKYEDLS 898

Query: 362 LKAREKFNMSVRSLPHPFTISEVARTWDVCAREVVSEYAERNGGGSFSSKYGSWEDCLHA 409
            + R +F++S++    P     +A+TW+ CAR  + EYAER+GGG+FSSKYG+WE  L A
Sbjct: 899 ARTRSRFSLSMKEFSEPGFSKGIAQTWNDCARATMVEYAERHGGGTFSSKYGAWETVLRA 935

BLAST of Lag0040609 vs. ExPASy Swiss-Prot
Match: F4I169 (Probable mediator of RNA polymerase II transcription subunit 15b OS=Arabidopsis thaliana OX=3702 GN=MED15B PE=3 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 6.2e-09
Identity = 47/130 (36.15%), Postives = 68/130 (52.31%), Query Frame = 0

Query: 67  DDFFCPQRGNMSHQMVNDAVHQEKAFI-----ISTPGMSPSTLLEEVTESDRE-----NA 126
           DD    Q  +MS Q     V Q +  +     I TPG+S S LL E+T  D        +
Sbjct: 122 DDQTTLQMQSMSMQGAGSRVQQIRQGVLQSLEIGTPGISASPLLPELTSPDGNIINPLTS 181

Query: 127 ICCVS-----PLQRLIKVVNVMSPKALSASVSDISSLVCMTDRVSSSAPLNKSKAAIGGD 182
            C  S     P++RLI+ +  +SP+ALS++V DI S+V M DR++ S P   S+A+ G D
Sbjct: 182 TCGKSSATELPIERLIRAMKSISPQALSSAVCDIRSVVSMVDRIAGSVPGKGSRASFGVD 241

BLAST of Lag0040609 vs. ExPASy TrEMBL
Match: A0A6J1CCM7 (mediator of RNA polymerase II transcription subunit 15a-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010000 PE=4 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 7.3e-154
Identity = 276/360 (76.67%), Postives = 305/360 (84.72%), Query Frame = 0

Query: 50  MLPGLPGNGRADYEA-AWDDFFCPQRGNMSHQMVNDAVHQEKAFIISTPGMSPSTLLEEV 109
           M+PGLP NGRAD E  AWDDF   QRG +SHQ+VNDAV +EK FIISTPGMSPS LLEEV
Sbjct: 1   MVPGLPENGRADCEEDAWDDFLFSQRGKVSHQIVNDAVQEEKPFIISTPGMSPSALLEEV 60

Query: 110 TESDRENAICCVSPLQRLIKVVNVMSPKALSASVSDISSLVCMTDRVSSSAPLNKSKAAI 169
           +ESDR+N IC V+PLQRL+K+VNVMSPKALSAS+SDI S+VCMTDRV+SSAPLNKSKA I
Sbjct: 61  SESDRDNDICSVTPLQRLLKMVNVMSPKALSASISDIDSIVCMTDRVASSAPLNKSKAEI 120

Query: 170 GGDLVGAVESRLRRNCSRFGGTIGSRRTRNYRSARMYASSRRLMDCEGFDLNSIFTQTSR 229
             DLVG V SRLR+ CS FGG+ GSRRTR+ R+ RMY SSRR+M CE +DLNS FT+ SR
Sbjct: 121 SEDLVGTVTSRLRKRCSIFGGSTGSRRTRHCRNTRMYTSSRRMMYCESYDLNSTFTERSR 180

Query: 230 VETPEHLHWHGFSLPVKQSLLEEIRRINEQLIDTVVSICDKDMRTTDTTATIDRSEGITV 289
           +ETPEH HW GFSLP KQ LLEEIR+INEQLIDTVVS+CDKDM TTD  AT DRSEGI V
Sbjct: 181 IETPEHSHWRGFSLPFKQYLLEEIRKINEQLIDTVVSVCDKDMITTDNAATNDRSEGIIV 240

Query: 290 ECSFVAMHTNSNLISQQTPVLQLAVHPLKLLVPKGYPHISPIFKKFHVEDCPTDCEDLSL 349
           ECSF AM  +SN ISQQ   LQL VHPLKLLVPKGYPH SPIF K  +E CPTDCE+LSL
Sbjct: 241 ECSFAAMRISSNKISQQASALQLTVHPLKLLVPKGYPHCSPIFNKLKLEACPTDCEELSL 300

Query: 350 KAREKFNMSVRSLPHPFTISEVARTWDVCAREVVSEYAERNGGGSFSSKYGSWEDCLHAA 409
           K REKFNM VRSLPHPF+ISEVARTWD CAREVVSE+A+RNGGGSF+SKYGSWEDCLH A
Sbjct: 301 KTREKFNMCVRSLPHPFSISEVARTWDACAREVVSEFAQRNGGGSFTSKYGSWEDCLHHA 360

BLAST of Lag0040609 vs. ExPASy TrEMBL
Match: A0A6J1JBE6 (mediator of RNA polymerase II transcription subunit 15a-like OS=Cucurbita maxima OX=3661 GN=LOC111482923 PE=4 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 1.6e-145
Identity = 275/373 (73.73%), Postives = 306/373 (82.04%), Query Frame = 0

Query: 39  FGKLAYRKRKTMLPGLPGNGRADYEAAWDDFFCPQRGNMSHQMVNDAVHQEKAFIISTPG 98
           F KLAYRK + MLPGL  NG  D+E A +DF  P        +VNDA   EKAFI+STPG
Sbjct: 65  FWKLAYRKWRIMLPGLLENGSVDFEKALEDFLYP-------PIVNDAAQAEKAFIVSTPG 124

Query: 99  MSPSTLLEEVTESDRENAICCVSPLQRLIKVVNVMSPKALSASVSDISSLVCMTDRVSSS 158
           MSPS+LLEEVT+SDR+N  CCV+PLQRLIK+VNVMSPKALSASVSDI S+VCMTDRVSSS
Sbjct: 125 MSPSSLLEEVTDSDRDNDKCCVTPLQRLIKMVNVMSPKALSASVSDIDSIVCMTDRVSSS 184

Query: 159 APLNKSKAAIGGDLVGAVESRLRRNCSRFGGTIGSRRTRNYRSARMYASSRRLMDCEGFD 218
           APLNKSK A+G DL G V SRLRR CSRFGGTIGSRRTR+YR          LMDCEGFD
Sbjct: 185 APLNKSKGAVGMDLAGTVASRLRRKCSRFGGTIGSRRTRHYRC---------LMDCEGFD 244

Query: 219 LNSIFTQTSR-VETPEHLHWHGFSLPVKQSLLEEIRRINEQLIDTVVSICDKDMRTTDTT 278
           L+S+FTQ SR +ETP+H +WHGFSLPVKQSLLEEIRRINEQLI T VSICDKDM TTD  
Sbjct: 245 LSSLFTQRSRIIETPKHSNWHGFSLPVKQSLLEEIRRINEQLIYTEVSICDKDMVTTDPA 304

Query: 279 ATIDRSEGITVECSFVAMHTNSNLISQQTPVLQLAVHPLKLLVPKGYPHISPIFKKFHVE 338
           ATI +SEGI VECSF A+ T S+LIS QT  +QLA+ PLKLLVP GYPHISPIFK   +E
Sbjct: 305 ATIGKSEGILVECSFGAVCTLSHLISHQTSAIQLAMQPLKLLVPIGYPHISPIFK---ME 364

Query: 339 DCPTDCEDLSLKAREKFNMSVRSLPHPFTISEVARTWDVCAREVVSEYAERNGGGSFSSK 398
            CPT CEDLSL+AREKFNMSVRSL HPF+ISE+ARTWD+CAR+V+SEYA +NGGGSFSSK
Sbjct: 365 ACPTGCEDLSLQAREKFNMSVRSLAHPFSISEMARTWDMCARDVISEYARQNGGGSFSSK 418

Query: 399 YGSWEDCLHAAWS 411
           YGSWEDCLH AWS
Sbjct: 425 YGSWEDCLHTAWS 418

BLAST of Lag0040609 vs. ExPASy TrEMBL
Match: A0A6J1GWK1 (mediator of RNA polymerase II transcription subunit 15a-like OS=Cucurbita moschata OX=3662 GN=LOC111458234 PE=4 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 2.1e-145
Identity = 276/373 (73.99%), Postives = 306/373 (82.04%), Query Frame = 0

Query: 39  FGKLAYRKRKTMLPGLPGNGRADYEAAWDDFFCPQRGNMSHQMVNDAVHQEKAFIISTPG 98
           F KLAY K + MLPGL  NG  D+E A ++F  P        +VNDA   EKAFI+STPG
Sbjct: 65  FWKLAYWKWRIMLPGLLENGSVDFEKALENFLYP-------PIVNDAAQAEKAFIVSTPG 124

Query: 99  MSPSTLLEEVTESDRENAICCVSPLQRLIKVVNVMSPKALSASVSDISSLVCMTDRVSSS 158
           MSPS+LLEEVT+SDR+N  CCV+PLQRLIK+VNVMSPKALSASVSDI S+VCMTDRVSSS
Sbjct: 125 MSPSSLLEEVTDSDRDNDKCCVTPLQRLIKMVNVMSPKALSASVSDIDSIVCMTDRVSSS 184

Query: 159 APLNKSKAAIGGDLVGAVESRLRRNCSRFGGTIGSRRTRNYRSARMYASSRRLMDCEGFD 218
           APLNKSK A+G DL G V SRLRR CSRFGGTIGSRRTR+YR          LMDCEGFD
Sbjct: 185 APLNKSKGAVGMDLAGTVASRLRRKCSRFGGTIGSRRTRHYRC---------LMDCEGFD 244

Query: 219 LNSIFTQTSR-VETPEHLHWHGFSLPVKQSLLEEIRRINEQLIDTVVSICDKDMRTTDTT 278
           L+S+FTQ SR +ETP+H +WHGFSLPVKQSLLEEIRRINEQLI T VSICDKDM TTD  
Sbjct: 245 LSSLFTQRSRIIETPKHSNWHGFSLPVKQSLLEEIRRINEQLIYTEVSICDKDMVTTDPG 304

Query: 279 ATIDRSEGITVECSFVAMHTNSNLISQQTPVLQLAVHPLKLLVPKGYPHISPIFKKFHVE 338
           ATI RSEGI VECSF A+ T S+LIS QT  +QLA+HPLKLLVP GYPHISPIFK   +E
Sbjct: 305 ATIGRSEGILVECSFGAVCTLSHLISHQTSAIQLAMHPLKLLVPIGYPHISPIFK---ME 364

Query: 339 DCPTDCEDLSLKAREKFNMSVRSLPHPFTISEVARTWDVCAREVVSEYAERNGGGSFSSK 398
            CPT CEDLSL+AREKFNMSVRSL HPF+ISEVARTWD+CAR+V+SEYA +NGGGSFSSK
Sbjct: 365 ACPTGCEDLSLQAREKFNMSVRSLAHPFSISEVARTWDMCARDVISEYARQNGGGSFSSK 418

Query: 399 YGSWEDCLHAAWS 411
           YGSWEDCLH AWS
Sbjct: 425 YGSWEDCLHTAWS 418

BLAST of Lag0040609 vs. ExPASy TrEMBL
Match: A0A6J1CEI3 (mediator of RNA polymerase II transcription subunit 15a-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111010000 PE=4 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 9.7e-114
Identity = 215/289 (74.39%), Postives = 239/289 (82.70%), Query Frame = 0

Query: 50  MLPGLPGNGRADYEA-AWDDFFCPQRGNMSHQMVNDAVHQEKAFIISTPGMSPSTLLEEV 109
           M+PGLP NGRAD E  AWDDF   QRG +SHQ+VNDAV +EK FIISTPGMSPS LLEEV
Sbjct: 1   MVPGLPENGRADCEEDAWDDFLFSQRGKVSHQIVNDAVQEEKPFIISTPGMSPSALLEEV 60

Query: 110 TESDRENAICCVSPLQRLIKVVNVMSPKALSASVSDISSLVCMTDRVSSSAPLNKSKAAI 169
           +ESDR+N IC V+PLQRL+K+VNVMSPKALSAS+SDI S+VCMTDRV+SSAPLNKSKA I
Sbjct: 61  SESDRDNDICSVTPLQRLLKMVNVMSPKALSASISDIDSIVCMTDRVASSAPLNKSKAEI 120

Query: 170 GGDLVGAVESRLRRNCSRFGGTIGSRRTRNYRSARMYASSRRLMDCEGFDLNSIFTQTSR 229
             DLVG V SRLR+ CS FGG+ GSRRTR+ R+ RMY SSRR+M CE +DLNS FT+ SR
Sbjct: 121 SEDLVGTVTSRLRKRCSIFGGSTGSRRTRHCRNTRMYTSSRRMMYCESYDLNSTFTERSR 180

Query: 230 VETPEHLHWHGFSLPVKQSLLEEIRRINEQLIDTVVSICDKDMRTTDTTATIDRSEGITV 289
           +ETPEH HW GFSLP KQ LLEEIR+INEQLIDTVVS+CDKDM TTD  AT DRSEGI V
Sbjct: 181 IETPEHSHWRGFSLPFKQYLLEEIRKINEQLIDTVVSVCDKDMITTDNAATNDRSEGIIV 240

Query: 290 ECSFVAMHTNSNLISQQTPVLQLAVHPLKLLVPKGYPHISPIFKKFHVE 338
           ECSF AM  +SN ISQQ   LQL VHPLKLLVPKGYPH SPIF K  +E
Sbjct: 241 ECSFAAMRISSNKISQQASALQLTVHPLKLLVPKGYPHCSPIFNKLKLE 289

BLAST of Lag0040609 vs. ExPASy TrEMBL
Match: A0A5B6ZEQ7 (Putative mediator of RNA polymerase II transcription subunit 15a (Fragment) OS=Davidia involucrata OX=16924 GN=Din_011975 PE=4 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 2.9e-65
Identity = 166/355 (46.76%), Postives = 214/355 (60.28%), Query Frame = 0

Query: 75   GNMSHQMVNDAVHQEKAFIISTPGMSPSTLLEEVTESD--RENAICCVS--------PLQ 134
            GN+ H     A+   ++  I TPG+S S LL E T  D    NA   VS        PL+
Sbjct: 960  GNIGHHQTTGALAPAQSLAIGTPGISASPLLAEFTSPDGNHGNASTIVSGKSSVTEQPLE 1019

Query: 135  RLIKVVNVMSPKALSASVSDISSLVCMTDRVSSSAPLNKSKAAIGGDLVGAVESRLR-RN 194
            RLIKVV  MSPKALSASVSDI S+V M DR++ SAP N S+AA+G DLV   + RL+ RN
Sbjct: 1020 RLIKVVKSMSPKALSASVSDIGSVVSMIDRIAGSAPGNGSRAAVGEDLVAMTKCRLQARN 1079

Query: 195  CSRFGGTIGSRRTRNYRSA----------RMYASSRRLMDCEGFDLNSIFTQTSRVETPE 254
             +   GT G+R+ R Y SA           +  S ++L   E  DL S  T TS ++ P 
Sbjct: 1080 FNTQDGTNGTRKMRRYTSAMPLNVVSSAGSINDSFKQLTGSEASDLES--TATSSIKRPR 1139

Query: 255  HLHWHGFSLPVKQSLLEEIRRINEQLIDTVVSICDKDMRTTDTTATIDRSEGITVECSFV 314
                    +    +LLEEIR IN+QLIDTVV I D+D+    T A  +  EG  V+CSF 
Sbjct: 1140 --------IEANHALLEEIREINQQLIDTVVDISDEDI--DPTAAASEGGEGTIVKCSFS 1199

Query: 315  AMHTNSNLISQQTPVLQLAVHPLKLLVPKGYPHISPI-FKKFHVEDCPTDCEDLSLKARE 374
            A+  + NL SQ        + PL+LLVP  YP+ SPI   KF +E    + EDLS+KA+ 
Sbjct: 1200 AVALSPNLKSQYASAQMSPIQPLRLLVPTNYPNCSPILLDKFPIE-VSKEYEDLSVKAKS 1259

Query: 375  KFNMSVRSLPHPFTISEVARTWDVCAREVVSEYAERNGGGSFSSKYGSWEDCLHA 408
            +F++S+RSL  P ++ E+ARTWDVCAR V+SEYA+++GGGSFSSKYGSWE CL A
Sbjct: 1260 RFSISLRSLSQPMSLGEIARTWDVCARAVISEYAQQSGGGSFSSKYGSWESCLSA 1301

BLAST of Lag0040609 vs. TAIR 10
Match: AT1G15780.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G10440.1); Has 103701 Blast hits to 43153 proteins in 1828 species: Archae - 30; Bacteria - 7385; Metazoa - 38639; Fungi - 11531; Plants - 7727; Viruses - 307; Other Eukaryotes - 38082 (source: NCBI BLink). )

HSP 1 Score: 190.7 bits (483), Expect = 2.4e-48
Identity = 130/349 (37.25%), Postives = 196/349 (56.16%), Query Frame = 0

Query: 75   GNMSHQMVNDAVHQEKAFIISTPGMSPSTLLEEVTESDRE--NAICCVS--------PLQ 134
            GN++ Q         ++  I TPG+S S LL+E T  D    N+    S        P++
Sbjct: 989  GNIARQQATGMQGVVQSLAIGTPGISASPLLQEFTSPDGNILNSSTITSGKPSATELPIE 1048

Query: 135  RLIKVVNVMSPKALSASVSDISSLVCMTDRVSSSAPLNKSKAAIGGDLVGAVESRLR-RN 194
            RLI+ V  +SP+ALS++VSDI S+V M DR++ SAP N S+A++G DLV   + RL+ RN
Sbjct: 1049 RLIRAVKSISPQALSSAVSDIGSVVSMVDRIAGSAPGNGSRASVGEDLVAMTKCRLQARN 1108

Query: 195  CSRFGGTIGSRRTRNYRSARMYASSRRLMDCEGFDLNSIF-TQTSRVETPEHLHWHGFSL 254
                 G + +++ + + +A M  S   L    G +      ++TS +E+           
Sbjct: 1109 FMTQEGMMATKKMKRHTTA-MPLSVASLGGSVGDNYKQFAGSETSDLESTATSDGKKART 1168

Query: 255  PVKQSLLEEIRRINEQLIDTVVSICDKDMRTTDTTATIDR--SEGITVECSFVAMHTNSN 314
              + +LLEEI+ IN++LIDTVV I D +     +   I     EG TV  SF+A+  +  
Sbjct: 1169 ETEHALLEEIKEINQRLIDTVVEISDDEDAADPSEVAISSIGCEGTTVRFSFIAVSLSPA 1228

Query: 315  LISQQTPVLQLAVHPLKLLVPKGYPHISP-IFKKFHVEDCPTDCEDLSLKAREKFNMSVR 374
            L +  +      + PL+LLVP  YP+ SP +  K  VE    + EDLS KA  +FN+ +R
Sbjct: 1229 LKAHLSSTQMSPIQPLRLLVPCSYPNGSPSLLDKLPVETSKEN-EDLSSKAMARFNILLR 1288

Query: 375  SLPHPFTISEVARTWDVCAREVVSEYAERNGGGSFSSKYGSWEDCLHAA 409
            SL  P ++ ++A+TWD CAR V+ EYA++ GGG+FSSKYG+WE  + A+
Sbjct: 1289 SLSQPMSLKDIAKTWDACARAVICEYAQQFGGGTFSSKYGTWEKYVAAS 1335

BLAST of Lag0040609 vs. TAIR 10
Match: AT2G10440.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G15780.1); Has 8319 Blast hits to 5104 proteins in 317 species: Archae - 0; Bacteria - 285; Metazoa - 1706; Fungi - 535; Plants - 320; Viruses - 18; Other Eukaryotes - 5455 (source: NCBI BLink). )

HSP 1 Score: 135.6 bits (340), Expect = 9.1e-32
Identity = 100/301 (33.22%), Postives = 158/301 (52.49%), Query Frame = 0

Query: 122 PLQRLIKVVNVMSPKALSASVSDISSLVCMTDRVSSSAPLN-KSKAAIGGDLVGAVESRL 181
           P+ RLIK     SPK+L+ SVS+ISS++ M D +  S P +  S+A +G DL        
Sbjct: 659 PIDRLIKAFQAASPKSLAESVSEISSVISMVDMIGGSFPSSGGSRAGLGEDL-------- 718

Query: 182 RRNCSRFGGTIGSRRTRNYRSARMYASSRRL---MDCEGFDLNS---IFTQTSRVETPEH 241
                       S RTRN+ +      S+R+   ++    D++S    + Q S +E+ E 
Sbjct: 719 ------------SERTRNFTTHEETNLSKRMKRSINIVPPDMSSQIDSYEQLSSLES-EV 778

Query: 242 LHWHGFSLPVKQ-----SLLEEIRRINEQLIDTVVSICDKDMRTTDTTATIDRSEGITVE 301
           +      L V       +LL+EI+  N +L++TVV ICD+D   T  T T      + + 
Sbjct: 779 VSTTSSGLKVNNIAPGYALLQEIKETNGRLVETVVEICDEDSLGTIVTCTY---APVALS 838

Query: 302 CSFVAMHTNSNLISQQTP-VLQLAVHPLKLLVPKGYPHISPI-FKKFHVEDCPTDCEDLS 361
            +F   + +  +I   +  ++Q  + PL+LL P  YP+ SPI  ++   +      EDLS
Sbjct: 839 ATFKDHYKSGKIIFYVSKCLMQAQIQPLRLLFPMDYPYSSPIVLEEISFDTSVHKYEDLS 898

Query: 362 LKAREKFNMSVRSLPHPFTISEVARTWDVCAREVVSEYAERNGGGSFSSKYGSWEDCLHA 409
            + R +F++S++    P     +A+TW+ CAR  + EYAER+GGG+FSSKYG+WE  L A
Sbjct: 899 ARTRSRFSLSMKEFSEPGFSKGIAQTWNDCARATMVEYAERHGGGTFSSKYGAWETVLRA 935

BLAST of Lag0040609 vs. TAIR 10
Match: AT2G10440.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G15780.1); Has 1628 Blast hits to 1350 proteins in 149 species: Archae - 0; Bacteria - 39; Metazoa - 480; Fungi - 159; Plants - 187; Viruses - 2; Other Eukaryotes - 761 (source: NCBI BLink). )

HSP 1 Score: 132.9 bits (333), Expect = 5.9e-31
Identity = 102/300 (34.00%), Postives = 155/300 (51.67%), Query Frame = 0

Query: 122 PLQRLIKVVNVMSPKALSASVSDISSLVCMTDRVSSSAPLN-KSKAAIGGDLVGAVESRL 181
           P+ RLIK     SPK+L+ SVS+ISS++ M D +  S P +  S+A +G DL        
Sbjct: 579 PIDRLIKAFQAASPKSLAESVSEISSVISMVDMIGGSFPSSGGSRAGLGEDL-------- 638

Query: 182 RRNCSRFGGTIGSRRTRNYRSARMYASSRRL---MDCEGFDLNS---IFTQTSRVETPEH 241
                       S RTRN+ +      S+R+   ++    D++S    + Q S +E+ E 
Sbjct: 639 ------------SERTRNFTTHEETNLSKRMKRSINIVPPDMSSQIDSYEQLSSLES-EV 698

Query: 242 LHWHGFSLPVKQ-----SLLEEIRRINEQLIDTVVSICDKDMRTTDTTATIDRSEGITVE 301
           +      L V       +LL+EI+  N +L++TVV ICD+D   T  T T      + + 
Sbjct: 699 VSTTSSGLKVNNIAPGYALLQEIKETNGRLVETVVEICDEDSLGTIVTCTY---APVALS 758

Query: 302 CSFVAMHTNSNLISQQTPVLQLAVHPLKLLVPKGYPHISPI-FKKFHVEDCPTDCEDLSL 361
            +F   H  S  I+Q        + PL+LL P  YP+ SPI  ++   +      EDLS 
Sbjct: 759 ATF-KDHYKSGKIAQ--------IQPLRLLFPMDYPYSSPIVLEEISFDTSVHKYEDLSA 818

Query: 362 KAREKFNMSVRSLPHPFTISEVARTWDVCAREVVSEYAERNGGGSFSSKYGSWEDCLHAA 409
           + R +F++S++    P     +A+TW+ CAR  + EYAER+GGG+FSSKYG+WE  L A+
Sbjct: 819 RTRSRFSLSMKEFSEPGFSKGIAQTWNDCARATMVEYAERHGGGTFSSKYGAWETVLRAS 845

BLAST of Lag0040609 vs. TAIR 10
Match: AT1G15770.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G15780.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 63.5 bits (153), Expect = 4.4e-10
Identity = 47/130 (36.15%), Postives = 68/130 (52.31%), Query Frame = 0

Query: 67  DDFFCPQRGNMSHQMVNDAVHQEKAFI-----ISTPGMSPSTLLEEVTESDRE-----NA 126
           DD    Q  +MS Q     V Q +  +     I TPG+S S LL E+T  D        +
Sbjct: 122 DDQTTLQMQSMSMQGAGSRVQQIRQGVLQSLEIGTPGISASPLLPELTSPDGNIINPLTS 181

Query: 127 ICCVS-----PLQRLIKVVNVMSPKALSASVSDISSLVCMTDRVSSSAPLNKSKAAIGGD 182
            C  S     P++RLI+ +  +SP+ALS++V DI S+V M DR++ S P   S+A+ G D
Sbjct: 182 TCGKSSATELPIERLIRAMKSISPQALSSAVCDIRSVVSMVDRIAGSVPGKGSRASFGVD 241