Lag0040523 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0040523
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionmitochondrial import inner membrane translocase subunit TIM14-1-like
Locationchr13: 5614491 .. 5615825 (+)
RNA-Seq ExpressionLag0040523
SyntenyLag0040523
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGCCAACCCTTGGAGGTTTCTTTGATGAACGATTATTATTGTGGCCTATCAAAAAGAGTTGAGGGGTACGGGTACCAAGTTTTTACTTTTCAATAGTTATTTGGATTTGTAAATGATATTTATGTTCATCTGCGAGTTCCAAGTTCTCATGTTGCAATAAGACTAACAAACTTCTAGGTGTGCATTAGGCTACACCATTCCTCGCAGGCCTTGCAGTAGCTGCTGCAGCTCTAGCTGGTAGATATGGAATTCGAGCGTGGCAAGCATTCAAGACACGGCCACCACAAGCCAGATCGCGCAAATTCTATGAAGGTGGTTTCTACCCTACGATGACAAAGAGGGAAGCGGCTCTTATTCTTGGTGTTAGGTAGTAGTCCAACATATACTTGAATTCGTGCTTCTTAAATAAATAAGAAAATTTTAGCTGGCTATTGATCTGCCATCATTCATAATATTACGTTATTGATTCTGACATTGTATCTTAAGCATTTTTTATAATCCAATCTTTTTAAGAAGTTAACAATTTGGTGCGGTTGACTTGAGTGGTAGGAAACTTAATGCCATGTAGTTATTGATAATTGCCCGTGGCTGTTTTATATCACATTCATATTTACAGGACAGAACCCAATTAGTAATTATTGCGCAAACACTGTCAGATTGAATTGATTTTCATTCTTTCTAATCCTTCTATTATTCAGGCACTGATTTACATTGCCCTCTAAGAAGGATTAGAACCTTTGCTTCTTTATGTCTTCTTTCCCCTCCATTTCATTATTGGAACCTTGGGGAACATTTGATCTAGATAATTAGTTTGGAGTACAAAGATCAGTGAGACACCAATAAGATATTTGGTTTTAATGTTTCATGAAATGATTATGAAATTACTTTCTTTTTTATGTAACACATCTGGAGAGCAATGCTTCTGTACTTCACTTCGATTGACTAGTGAAAACATGACATCTTAAGCAACGTCCACTTGTACATATTGCCTTTATTGTCGAGAAAAACAAAATAACTACTAGTGTTTTGTCTTTACTTTTCCACCATAAACTTCTTGTTGAATGAAAGAACACTGTGGGAGACATATCCGTCTTTGTTTATCAATTGCTTCATTCACGCCATTAAGAAGATTACTTTGTAGGGATTGACCGAATTTATCTTTGATGGTTAACAGAGAAAATACACCTCCAGATAAAATCAAGGAAGCACATAGAAGAGTCATGATCGCGAACCATCCCGATGCGGGTGGCAGTCATTATCTTGCTTCAAAGATCAATGAAGCCAAGGATGTGCTGCTGGGCAAATCTAAAGGCAGTGGATCGGCTTTTTAA

mRNA sequence

ATGGGGCCAACCCTTGGAGGCCTTGCAGTAGCTGCTGCAGCTCTAGCTGGTAGATATGGAATTCGAGCGTGGCAAGCATTCAAGACACGGCCACCACAAGCCAGATCGCGCAAATTCTATGAAGGTGGTTTCTACCCTACGATGACAAAGAGGGAAGCGGCTCTTATTCTTGGTGTTAGAGAAAATACACCTCCAGATAAAATCAAGGAAGCACATAGAAGAGTCATGATCGCGAACCATCCCGATGCGGGTGGCAGTCATTATCTTGCTTCAAAGATCAATGAAGCCAAGGATGTGCTGCTGGGCAAATCTAAAGGCAGTGGATCGGCTTTTTAA

Coding sequence (CDS)

ATGGGGCCAACCCTTGGAGGCCTTGCAGTAGCTGCTGCAGCTCTAGCTGGTAGATATGGAATTCGAGCGTGGCAAGCATTCAAGACACGGCCACCACAAGCCAGATCGCGCAAATTCTATGAAGGTGGTTTCTACCCTACGATGACAAAGAGGGAAGCGGCTCTTATTCTTGGTGTTAGAGAAAATACACCTCCAGATAAAATCAAGGAAGCACATAGAAGAGTCATGATCGCGAACCATCCCGATGCGGGTGGCAGTCATTATCTTGCTTCAAAGATCAATGAAGCCAAGGATGTGCTGCTGGGCAAATCTAAAGGCAGTGGATCGGCTTTTTAA

Protein sequence

MGPTLGGLAVAAAALAGRYGIRAWQAFKTRPPQARSRKFYEGGFYPTMTKREAALILGVRENTPPDKIKEAHRRVMIANHPDAGGSHYLASKINEAKDVLLGKSKGSGSAF
Homology
[Close]
BLAST of Lag0040523 vs. NCBI nr
Match: XP_022966723.1 (mitochondrial import inner membrane translocase subunit TIM14-1-like isoform X2 [Cucurbita maxima] >XP_023541262.1 mitochondrial import inner membrane translocase subunit TIM14-1-like [Cucurbita pepo subsp. pepo] >XP_023541263.1 mitochondrial import inner membrane translocase subunit TIM14-1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 205.7 bits (522), Expect = 2.1e-49
Identity = 104/109 (95.41%), Postives = 105/109 (96.33%), Query Frame = 0 

Query: 3   PTLGGLAVAAAALAGRYGIRAWQAFKTRPPQARSRKFYEGGFYPTMTKREAALILGVREN 62
           P L GLAVAAAALAGRYGIRAWQAFKTRPPQARSRKFYEGGF PTMTKREAALILGVREN
Sbjct: 4   PFLAGLAVAAAALAGRYGIRAWQAFKTRPPQARSRKFYEGGFNPTMTKREAALILGVREN 63

Query: 63  TPPDKIKEAHRRVMIANHPDAGGSHYLASKINEAKDVLLGKSKGSGSAF 112
           TPP+KIKEAHRRVMIANHPDAGGSHYLASKINEAKDVLLGKSKGS SAF
Sbjct: 64  TPPEKIKEAHRRVMIANHPDAGGSHYLASKINEAKDVLLGKSKGSPSAF 112

[Close]
BLAST of Lag0040523 vs. NCBI nr
Match: KAG6573825.1 (Mitochondrial import inner membrane translocase subunit TIM14-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 205.7 bits (522), Expect = 2.1e-49
Identity = 104/109 (95.41%), Postives = 105/109 (96.33%), Query Frame = 0 

Query: 3   PTLGGLAVAAAALAGRYGIRAWQAFKTRPPQARSRKFYEGGFYPTMTKREAALILGVREN 62
           P L GLAVAAAALAGRYGIRAWQAFKTRPPQARSRKFYEGGF PTMTKREAALILGVREN
Sbjct: 485 PFLAGLAVAAAALAGRYGIRAWQAFKTRPPQARSRKFYEGGFNPTMTKREAALILGVREN 544

Query: 63  TPPDKIKEAHRRVMIANHPDAGGSHYLASKINEAKDVLLGKSKGSGSAF 112
           TPP+KIKEAHRRVMIANHPDAGGSHYLASKINEAKDVLLGKSKGS SAF
Sbjct: 545 TPPEKIKEAHRRVMIANHPDAGGSHYLASKINEAKDVLLGKSKGSPSAF 593

[Close]
BLAST of Lag0040523 vs. NCBI nr
Match: XP_022966720.1 (mitochondrial import inner membrane translocase subunit TIM14-1-like isoform X1 [Cucurbita maxima] >XP_022966722.1 mitochondrial import inner membrane translocase subunit TIM14-1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 205.7 bits (522), Expect = 2.1e-49
Identity = 104/109 (95.41%), Postives = 105/109 (96.33%), Query Frame = 0 

Query: 3   PTLGGLAVAAAALAGRYGIRAWQAFKTRPPQARSRKFYEGGFYPTMTKREAALILGVREN 62
           P L GLAVAAAALAGRYGIRAWQAFKTRPPQARSRKFYEGGF PTMTKREAALILGVREN
Sbjct: 22  PFLAGLAVAAAALAGRYGIRAWQAFKTRPPQARSRKFYEGGFNPTMTKREAALILGVREN 81

Query: 63  TPPDKIKEAHRRVMIANHPDAGGSHYLASKINEAKDVLLGKSKGSGSAF 112
           TPP+KIKEAHRRVMIANHPDAGGSHYLASKINEAKDVLLGKSKGS SAF
Sbjct: 82  TPPEKIKEAHRRVMIANHPDAGGSHYLASKINEAKDVLLGKSKGSPSAF 130

[Close]
BLAST of Lag0040523 vs. NCBI nr
Match: XP_022944881.1 (mitochondrial import inner membrane translocase subunit TIM14-1-like [Cucurbita moschata])

HSP 1 Score: 204.1 bits (518), Expect = 6.1e-49
Identity = 103/109 (94.50%), Postives = 105/109 (96.33%), Query Frame = 0 

Query: 3   PTLGGLAVAAAALAGRYGIRAWQAFKTRPPQARSRKFYEGGFYPTMTKREAALILGVREN 62
           P L GLAVAAAALAGRYGIRAWQAFKTRPPQARSRKFYEGGF PTMTKREAALILGVREN
Sbjct: 67  PFLAGLAVAAAALAGRYGIRAWQAFKTRPPQARSRKFYEGGFNPTMTKREAALILGVREN 126

Query: 63  TPPDKIKEAHRRVMIANHPDAGGSHYLASKINEAKDVLLGKSKGSGSAF 112
           TPP+KIKEAHRRVMIANHPDAGGSHYLASKINEAKDVLLGKS+GS SAF
Sbjct: 127 TPPEKIKEAHRRVMIANHPDAGGSHYLASKINEAKDVLLGKSEGSPSAF 175

[Close]
BLAST of Lag0040523 vs. NCBI nr
Match: XP_038892473.1 (mitochondrial import inner membrane translocase subunit TIM14-1-like [Benincasa hispida])

HSP 1 Score: 203.8 bits (517), Expect = 7.9e-49
Identity = 101/109 (92.66%), Postives = 104/109 (95.41%), Query Frame = 0 

Query: 3   PTLGGLAVAAAALAGRYGIRAWQAFKTRPPQARSRKFYEGGFYPTMTKREAALILGVREN 62
           P L GLAVAAAALAGRYGIRAWQAFK+RPPQARSRKFYEGGFYPTMT+REAALILG+REN
Sbjct: 4   PLLAGLAVAAAALAGRYGIRAWQAFKSRPPQARSRKFYEGGFYPTMTRREAALILGIREN 63

Query: 63  TPPDKIKEAHRRVMIANHPDAGGSHYLASKINEAKDVLLGKSKGSGSAF 112
             PDKIKEAHRRVMIANHPDAGGSHYLASKINEAKDVLLGKSK SGSAF
Sbjct: 64  ATPDKIKEAHRRVMIANHPDAGGSHYLASKINEAKDVLLGKSKSSGSAF 112

[Close]
BLAST of Lag0040523 vs. ExPASy Swiss-Prot
Match: Q8RV04 (Mitochondrial import inner membrane translocase subunit TIM14-1 OS=Arabidopsis thaliana OX=3702 GN=TIM14-1 PE=1 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 1.8e-43
Identity = 84/109 (77.06%), Postives = 98/109 (89.91%), Query Frame = 0 

Query: 3   PTLGGLAVAAAALAGRYGIRAWQAFKTRPPQARSRKFYEGGFYPTMTKREAALILGVREN 62
           P + G+AVAA ALAGRYGI+AWQAFK RPP+ + +KFYEGGF PTMTKREAALILGVRE+
Sbjct: 4   PFIAGVAVAATALAGRYGIQAWQAFKARPPRPKIKKFYEGGFQPTMTKREAALILGVRES 63

Query: 63  TPPDKIKEAHRRVMIANHPDAGGSHYLASKINEAKDVLLGKSKGSGSAF 112
              +K+KEAHR+VM+ANHPDAGGSH+LASKINEAKDV+LGK+K SGSAF
Sbjct: 64  VAAEKVKEAHRKVMVANHPDAGGSHFLASKINEAKDVMLGKTKNSGSAF 112

[Close]
BLAST of Lag0040523 vs. ExPASy Swiss-Prot
Match: Q9LYY2 (Mitochondrial import inner membrane translocase subunit TIM14-3 OS=Arabidopsis thaliana OX=3702 GN=TIM14-3 PE=1 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 4.9e-41
Identity = 83/109 (76.15%), Postives = 93/109 (85.32%), Query Frame = 0 

Query: 3   PTLGGLAVAAAALAGRYGIRAWQAFKTRPPQARSRKFYEGGFYPTMTKREAALILGVREN 62
           P + G AVAAAA+AGRYGI AWQAFK RP   R R+FYEGGF  +MT+REAALILGVRE+
Sbjct: 4   PMIAGAAVAAAAVAGRYGILAWQAFKARPRVPRMRRFYEGGFQSSMTRREAALILGVRES 63

Query: 63  TPPDKIKEAHRRVMIANHPDAGGSHYLASKINEAKDVLLGKSKGSGSAF 112
              DK+KEAHRRVM+ANHPDAGGSHYLASKINEAKD++LGKS  SGSAF
Sbjct: 64  VVADKVKEAHRRVMVANHPDAGGSHYLASKINEAKDMMLGKSNNSGSAF 112

[Close]
BLAST of Lag0040523 vs. ExPASy Swiss-Prot
Match: Q9SF33 (Mitochondrial import inner membrane translocase subunit TIM14-2 OS=Arabidopsis thaliana OX=3702 GN=TIM14-2 PE=3 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 3.1e-40
Identity = 81/107 (75.70%), Postives = 92/107 (85.98%), Query Frame = 0 

Query: 5   LGGLAVAAAALAGRYGIRAWQAFKTRPPQARSRKFYEGGFYPTMTKREAALILGVRENTP 64
           + G AVAAAA AG+YGI AWQAFK RP + R RKFYEGGF  TM +REAALILGVRE+  
Sbjct: 6   IAGAAVAAAAYAGKYGIEAWQAFKLRPVRPRMRKFYEGGFQATMNRREAALILGVRESVA 65

Query: 65  PDKIKEAHRRVMIANHPDAGGSHYLASKINEAKDVLLGKSKGSGSAF 112
            +K+KEAHRRVM+ANHPDAGGSHYLASKINEAKD++LGK+K SGSAF
Sbjct: 66  AEKVKEAHRRVMVANHPDAGGSHYLASKINEAKDMMLGKTKNSGSAF 112

[Close]
BLAST of Lag0040523 vs. ExPASy Swiss-Prot
Match: Q3ZBN8 (Mitochondrial import inner membrane translocase subunit TIM14 OS=Bos taurus OX=9913 GN=DNAJC19 PE=3 SV=3)

HSP 1 Score: 109.8 bits (273), Expect = 2.0e-23
Identity = 58/107 (54.21%), Postives = 73/107 (68.22%), Query Frame = 0 

Query: 7   GLAVAAAALAGRYGIRAW--------QAFKTRPPQARSRKFYEGGFYPTMTKREAALILG 66
           GL +AAA  AGRY ++A         Q F++ P  A S  +Y GGF P MTKREAALILG
Sbjct: 9   GLTIAAAGFAGRYALQAMKHMEPQVKQVFQSLPKTAFSGGYYRGGFEPKMTKREAALILG 68

Query: 67  VRENTPPDKIKEAHRRVMIANHPDAGGSHYLASKINEAKDVLLGKSK 106
           V       KI++AHRR+M+ NHPD GGS Y+A+KINEAKD+L G++K
Sbjct: 69  VSPTANKAKIRDAHRRIMLLNHPDKGGSPYIAAKINEAKDLLEGQAK 115

[Close]
BLAST of Lag0040523 vs. ExPASy Swiss-Prot
Match: Q96DA6 (Mitochondrial import inner membrane translocase subunit TIM14 OS=Homo sapiens OX=9606 GN=DNAJC19 PE=1 SV=3)

HSP 1 Score: 109.4 bits (272), Expect = 2.7e-23
Identity = 58/107 (54.21%), Postives = 73/107 (68.22%), Query Frame = 0 

Query: 7   GLAVAAAALAGRYGIRAW--------QAFKTRPPQARSRKFYEGGFYPTMTKREAALILG 66
           GL +AAA  AGRY ++A         Q F++ P  A S  +Y GGF P MTKREAALILG
Sbjct: 9   GLTIAAAGFAGRYVLQAMKHMEPQVKQVFQSLPKSAFSGGYYRGGFEPKMTKREAALILG 68

Query: 67  VRENTPPDKIKEAHRRVMIANHPDAGGSHYLASKINEAKDVLLGKSK 106
           V       KI++AHRR+M+ NHPD GGS Y+A+KINEAKD+L G++K
Sbjct: 69  VSPTANKGKIRDAHRRIMLLNHPDKGGSPYIAAKINEAKDLLEGQAK 115

The following BLAST results are available for this feature:
BLAST of Lag0040523 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022966723.12.1e-4995.41mitochondrial import inner membrane translocase subunit TIM14-1-like isoform X2 ... [more]
KAG6573825.12.1e-4995.41Mitochondrial import inner membrane translocase subunit TIM14-1, partial [Cucurb... [more]
XP_022966720.12.1e-4995.41mitochondrial import inner membrane translocase subunit TIM14-1-like isoform X1 ... [more]
XP_022944881.16.1e-4994.50mitochondrial import inner membrane translocase subunit TIM14-1-like [Cucurbita ... [more]
XP_038892473.17.9e-4992.66mitochondrial import inner membrane translocase subunit TIM14-1-like [Benincasa ... [more]
BLAST of Lag0040523 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8RV041.8e-4377.06Mitochondrial import inner membrane translocase subunit TIM14-1 OS=Arabidopsis t... [more]
Q9LYY24.9e-4176.15Mitochondrial import inner membrane translocase subunit TIM14-3 OS=Arabidopsis t... [more]
Q9SF333.1e-4075.70Mitochondrial import inner membrane translocase subunit TIM14-2 OS=Arabidopsis t... [more]
Q3ZBN82.0e-2354.21Mitochondrial import inner membrane translocase subunit TIM14 OS=Bos taurus OX=9... [more]
Q96DA62.7e-2354.21Mitochondrial import inner membrane translocase subunit TIM14 OS=Homo sapiens OX... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001623DnaJ domainSMARTSM00271dnaj_3coord: 51..105
e-value: 1.4E-5
score: 34.5
IPR001623DnaJ domainPROSITEPS50076DNAJ_2coord: 52..105
score: 8.961946
IPR001623DnaJ domainCDDcd06257DnaJcoord: 56..100
e-value: 1.03078E-7
score: 43.3029
IPR036869Chaperone J-domain superfamilyGENE3D1.10.287.110DnaJ domaincoord: 38..105
e-value: 1.1E-28
score: 100.9
IPR036869Chaperone J-domain superfamilySUPERFAMILY46565Chaperone J-domaincoord: 42..103
NoneNo IPR availablePANTHERPTHR12763UNCHARACTERIZEDcoord: 3..111
NoneNo IPR availablePANTHERPTHR12763:SF51MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM14-3coord: 3..111

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0040523.1Lag0040523.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030150 protein import into mitochondrial matrix
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0001405 PAM complex, Tim23 associated import motor
molecular_function GO:0001671 ATPase activator activity