Lag0040471 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0040471
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	protein NUCLEAR FUSION DEFECTIVE 4
Location	chr13: 5137824 .. 5141773 (-)
RNA-Seq Expression	Lag0040471
Synteny	Lag0040471

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATCGGCGCCGGTCCGGGAGGCGGCGGAGGAATCTCCAGATGGTCCGACATAAGAACCATTGCCGTCCACGTAATCACCGGGCGGTGGTTCGTGGTGTTCGCTTCGCTTCTAATCATGGCGGCCAACGGCGGCATCTTCATGTTCGGCCTCTATTCCAACGATATAAAGTCCGTTTTGGGCTACGATCAAACCACGTTGAATTTGTTGAGCTTTTTCAAGGATTTGGGTGCAAACGTCGGCGTTTTGTCCGGTCTAATCAATGAAGTTTCGCCGCCGTGGGTGGTTCTGTCCAGCGGCGCCGCCCTGAATTTCTTTGGTTACTTCATGATTTGGCTGGCCGTTACTAAGAGGATCTCCACCCCCAAAGTTTGGCAAATGTGTGTATACATTTGTATTGGAGGTAATTCTCAGTCATTTGCTAACACCGGTTCGCTTGTTACTTGCGTCAAGAACTTCCCCGAGAGTCGGGGTATCGTTATCGGTGAGTGATTATCAATATATATTTTTTATGTTCATGTTGGCGAGTGATTATCAATTTCAATTATTCAAGAATTGATATTTAATTTCAACTATGTTTAGATTGACTATTGTTTCGATATTATGCTAAGGACAATACTCTTTCTTTTTAACCTCTAAACGAGGGTCATACTAAATACCAATAAGCTACTTTGGCATTCCTAAGGACAATACTCTTGAGACTAATTAGAAAAGTAGAAAAAATGAGGAAAGGTGAAAATAATTTTTTTAAAAATTAAGCATGCATGGTGTCTCTTCAAGAAACCCACCACTTATGGCGTTATGTATTTTTTTTCTCCTGATTCAACAACATTTGAAGGTTGGAGGATTTGAACATACGACCTCTTAATCAATAATACATGTCAATTACCGGTGAACTATGCTTGCGCGTGGGTTTGTATCTTGAAGAGAACACGACACAACCAAATTTGGAGATTTGGATTTTTAACATCACGTGTCCTTTTTTTATTAAGTGAACACAATCATTTTTTTAAACCGTTTTTTTTTTAATCTTTTTTTATCCAATTTTCAGATTTTTTATATTTGGAGATTTTGCATCGTTTTTTTTAATCTTGATATATATATATATATATATATATATATATAAACCATAAACCCTTAAGATGTACAAAAATCTAACGTAACAAAAACACCACACATTAAATTCAACAAGAATGTGTAGTTGAATATAATCATGAGATAAATATAATATAATATAATTTATATATCCTCATCATTCTTTTTTTTAATTTATTTATTATGATTATTTTTTTAAATACTTATTTATTACTAGTTAATTAAAGTTTTTTTTTTTTAAAAAAAAAACACACATTAAATTCAACCAAGAATGATAAAATCTTATTTGTAGTTGTAAATTTGATTAATCTTATTACTAGCTTGAAGAGTGATGGTTCAAAGATTTGTCAAAAATTTTAAGGCATTATTTTCCAAAGAAATCTAAATGTTTATTTGCTATTTACATCTTTTAAGGATGGTTAGTTTTTAAATTTTAATTTTGTTTTCTATTTAAACTTAAATTTAATTGTGTTTTAACTCTATGAACTTTGAAAAATTCGTGGAACTAACGGGCATGGGAGGATCTATTCATGACATTTGTTGAGTTTAATGTGTAGTGTTTTTTAAATAAAAACTTTAACTAATTAGTAATACATACTAATTGAAAAAAGAAAAATAATTATAGTAAATAAAAAAAGGAGAGATATAAATTAAATTATAGATATATATAAACAAAAAAAATGAAAAAAAAAACAATGTAAAATCTCTAAATCTAAAAAATCTGAAAATTGGATAAATAAAAAGAAAAAAACGGGTTTAAAAAAATAAAAAGAAACATAATTATTAAAAAAAAAAAAAACAAGAGGACACGTGGCGTTAGAAAACAAAATCTCAACAAATTTGGATGTGTTGCGTCTCTTGAAAATAAGCAACCCACTAAACGACAAGTGGCTGCTATCTTGAAGAGACGCATCGGTTGGTTTTTCTAATTACTTTCATTTTTTTTTATTTTTAATTAGTCCAATACTTGAATAGGTAGGGATCTCTCTAAAATCTTGACAAGTAAGAGCCAGAGATAGAAAATGAGAATTTGTTTTTTTTTTTCTTTTTTCTTTTTTTTTTTTTTTTTATCTTTTGTAATTGTCAATGTTTTAATGTTGGTGTGTCCTCGAATATACAACTAAATATTGTCCTAAATTATAATCTTGATAGTTTTTCTTTCGAGTTCGTGAGAAGAACAAAAGTTATTTGCATTAATACTACCATGAATTTGACAGCTATTTTTTTATACAAAAAGAAAAATATCTCTTTTCTCTCTCACTTCTTTGTATTTTCCCTCCTACTAGTTGAAGAGAAATCATTAGGGCCACTGCAATGAAATGGATAGTTTCACATGGTTGATGTAAGAGAAAAATTATAAAAGTATCTCGATTTTATATGGTTATCAGAGAGCGGACAAATTTGAAAATGTACAATAATTTTAAAGAGGGAGTCTCATGGCACCTAGAGCACCCAAAGTTGTAGACTTAATCTTGATTAGTTGAAGATTTTTACTAAATATTTTTAGGATGATATTTGGTTGTATACGTAAATTTAATCATTTATTCATATTGTATGCAGGTATCTTAAAGGGCTACTTCGGCCTAAGCGGCGCCATAACCACACAACTCTTCCACGCGTTCTACGGCTCCGACACCAAATCCTTAATCCTCCTCCTCGGATGGCTCCCGGCGGCAATATCCTTCGCATTTCTCCGAACAATTCGGATCATGAAAGTCATCCGACACCCAAACGAGCTCAAAGTCTTCTACAATTTCCTCTACATTTCTCTCGCCCTCGCCGGATTCCTAATGCTAATGATCATCATAGAAAGCAAACTAGTGTTAACTCAAAGCGCATACGGCGGCAGCGCCACCGTCGTCCTCCTCCTCCTCTTCCTCCCCCTCGCCGTCGTTGTAATCGAAGAATACAACCTCTGGAAACTCAAAACCGCCGCCATTGGAGCCCCAAATCCTATTCAAATCATAACAGAGAAGCCAAATATCCCAAAAACAGAGCATCAGAAACAAACCCAACAAAATCAAAAACAAAAGCAACCATCTTGCTGGACGACGATCTTCAGCCCGCCGGAACGCGGCGAGGATTTCACGATTCTCCAAGCTCTCTTCAGCATGGACATGTTGGTTCTCTTCTTCTCTGCAACCTGCGGCGTCGGCGGCACGTTGACGGTGATCGACAATCTGGGTCAAATCGGAATGTCATTGGGATATCCAAAAAGGAGCATCGGCACATTCGTGACACTCGTGAGCATTTGGAATTACCTCGGCCGAGTCGCCTCTGGTTTCATCTCTGAAATTGTTCTAACCAAATACAAATTCCCTCGTCCTTTGATGCTTTCTTTGATGCTCCTTTTCTCTTGCGCCGGTTATTTGATGGTCGCTTTCGATGTCCAAAATGGGCTCTACGCAGCTTCGATTGTGATCGGATTCTGCTTCGGCGCGCAGTGGCCGTTGATTTACGCTATAATTTCGGAGCTTTTCGGGTTGAAGTATTACTCCACGCTTTTCAATTTCGGGTCAGTGGCGAGCCCAATTGGGCTTTATGTTCTGAACGTGAAAGTGAAGGGGAATTTCTACGACAGAGAGGCGGAGAAGCAGCTGAGAGCAAAAGGGCTTGTGAGAAAAGCAGGGGAGGAGCTGAAGTGCTTTGGAGCAGAGTGCTTTAAGCTGTCGTTTATTGTGATTACGGGAGTTACTTTGTTGGGTATGCTCGTTTCGTTGATTTTAGTGATCAGAACGAGGAGTTTTTACAAGAGTGATATTTACAAGAAGTTCAGAGAGGAAGCTGAAACAGAGGTGGCCGGAAATGGCGCGGTGGTGGCCGCCGGAGCGCCGGAGGAGACAAGAAGGCAATAG

mRNA sequence

ATGATCGGCGCCGGTCCGGGAGGCGGCGGAGGAATCTCCAGATGGTCCGACATAAGAACCATTGCCGTCCACGTAATCACCGGGCGGTGGTTCGTGGTGTTCGCTTCGCTTCTAATCATGGCGGCCAACGGCGGCATCTTCATGTTCGGCCTCTATTCCAACGATATAAAGTCCGTTTTGGGCTACGATCAAACCACGTTGAATTTGTTGAGCTTTTTCAAGGATTTGGGTGCAAACGTCGGCGTTTTGTCCGGTCTAATCAATGAAGTTTCGCCGCCGTGGGTGGTTCTGTCCAGCGGCGCCGCCCTGAATTTCTTTGGTTACTTCATGATTTGGCTGGCCGTTACTAAGAGGATCTCCACCCCCAAAGTTTGGCAAATGTGTGTATACATTTGTATTGGAGGTAATTCTCAGTCATTTGCTAACACCGGTTCGCTTGTTACTTGCGTCAAGAACTTCCCCGAGAGTCGGGGTATCGTTATCGGTATCTTAAAGGGCTACTTCGGCCTAAGCGGCGCCATAACCACACAACTCTTCCACGCGTTCTACGGCTCCGACACCAAATCCTTAATCCTCCTCCTCGGATGGCTCCCGGCGGCAATATCCTTCGCATTTCTCCGAACAATTCGGATCATGAAAGTCATCCGACACCCAAACGAGCTCAAAGTCTTCTACAATTTCCTCTACATTTCTCTCGCCCTCGCCGGATTCCTAATGCTAATGATCATCATAGAAAGCAAACTAGTGTTAACTCAAAGCGCATACGGCGGCAGCGCCACCGTCGTCCTCCTCCTCCTCTTCCTCCCCCTCGCCGTCGTTGTAATCGAAGAATACAACCTCTGGAAACTCAAAACCGCCGCCATTGGAGCCCCAAATCCTATTCAAATCATAACAGAGAAGCCAAATATCCCAAAAACAGAGCATCAGAAACAAACCCAACAAAATCAAAAACAAAAGCAACCATCTTGCTGGACGACGATCTTCAGCCCGCCGGAACGCGGCGAGGATTTCACGATTCTCCAAGCTCTCTTCAGCATGGACATGTTGGTTCTCTTCTTCTCTGCAACCTGCGGCGTCGGCGGCACGTTGACGGTGATCGACAATCTGGGTCAAATCGGAATGTCATTGGGATATCCAAAAAGGAGCATCGGCACATTCGTGACACTCGTGAGCATTTGGAATTACCTCGGCCGAGTCGCCTCTGGTTTCATCTCTGAAATTGTTCTAACCAAATACAAATTCCCTCGTCCTTTGATGCTTTCTTTGATGCTCCTTTTCTCTTGCGCCGGTTATTTGATGGTCGCTTTCGATGTCCAAAATGGGCTCTACGCAGCTTCGATTGTGATCGGATTCTGCTTCGGCGCGCAGTGGCCGTTGATTTACGCTATAATTTCGGAGCTTTTCGGGTTGAAGTATTACTCCACGCTTTTCAATTTCGGGTCAGTGGCGAGCCCAATTGGGCTTTATGTTCTGAACGTGAAAGTGAAGGGGAATTTCTACGACAGAGAGGCGGAGAAGCAGCTGAGAGCAAAAGGGCTTGTGAGAAAAGCAGGGGAGGAGCTGAAGTGCTTTGGAGCAGAGTGCTTTAAGCTGTCGTTTATTGTGATTACGGGAGTTACTTTGTTGGGTATGCTCGTTTCGTTGATTTTAGTGATCAGAACGAGGAGTTTTTACAAGAGTGATATTTACAAGAAGTTCAGAGAGGAAGCTGAAACAGAGGTGGCCGGAAATGGCGCGGTGGTGGCCGCCGGAGCGCCGGAGGAGACAAGAAGGCAATAG

Coding sequence (CDS)

ATGATCGGCGCCGGTCCGGGAGGCGGCGGAGGAATCTCCAGATGGTCCGACATAAGAACCATTGCCGTCCACGTAATCACCGGGCGGTGGTTCGTGGTGTTCGCTTCGCTTCTAATCATGGCGGCCAACGGCGGCATCTTCATGTTCGGCCTCTATTCCAACGATATAAAGTCCGTTTTGGGCTACGATCAAACCACGTTGAATTTGTTGAGCTTTTTCAAGGATTTGGGTGCAAACGTCGGCGTTTTGTCCGGTCTAATCAATGAAGTTTCGCCGCCGTGGGTGGTTCTGTCCAGCGGCGCCGCCCTGAATTTCTTTGGTTACTTCATGATTTGGCTGGCCGTTACTAAGAGGATCTCCACCCCCAAAGTTTGGCAAATGTGTGTATACATTTGTATTGGAGGTAATTCTCAGTCATTTGCTAACACCGGTTCGCTTGTTACTTGCGTCAAGAACTTCCCCGAGAGTCGGGGTATCGTTATCGGTATCTTAAAGGGCTACTTCGGCCTAAGCGGCGCCATAACCACACAACTCTTCCACGCGTTCTACGGCTCCGACACCAAATCCTTAATCCTCCTCCTCGGATGGCTCCCGGCGGCAATATCCTTCGCATTTCTCCGAACAATTCGGATCATGAAAGTCATCCGACACCCAAACGAGCTCAAAGTCTTCTACAATTTCCTCTACATTTCTCTCGCCCTCGCCGGATTCCTAATGCTAATGATCATCATAGAAAGCAAACTAGTGTTAACTCAAAGCGCATACGGCGGCAGCGCCACCGTCGTCCTCCTCCTCCTCTTCCTCCCCCTCGCCGTCGTTGTAATCGAAGAATACAACCTCTGGAAACTCAAAACCGCCGCCATTGGAGCCCCAAATCCTATTCAAATCATAACAGAGAAGCCAAATATCCCAAAAACAGAGCATCAGAAACAAACCCAACAAAATCAAAAACAAAAGCAACCATCTTGCTGGACGACGATCTTCAGCCCGCCGGAACGCGGCGAGGATTTCACGATTCTCCAAGCTCTCTTCAGCATGGACATGTTGGTTCTCTTCTTCTCTGCAACCTGCGGCGTCGGCGGCACGTTGACGGTGATCGACAATCTGGGTCAAATCGGAATGTCATTGGGATATCCAAAAAGGAGCATCGGCACATTCGTGACACTCGTGAGCATTTGGAATTACCTCGGCCGAGTCGCCTCTGGTTTCATCTCTGAAATTGTTCTAACCAAATACAAATTCCCTCGTCCTTTGATGCTTTCTTTGATGCTCCTTTTCTCTTGCGCCGGTTATTTGATGGTCGCTTTCGATGTCCAAAATGGGCTCTACGCAGCTTCGATTGTGATCGGATTCTGCTTCGGCGCGCAGTGGCCGTTGATTTACGCTATAATTTCGGAGCTTTTCGGGTTGAAGTATTACTCCACGCTTTTCAATTTCGGGTCAGTGGCGAGCCCAATTGGGCTTTATGTTCTGAACGTGAAAGTGAAGGGGAATTTCTACGACAGAGAGGCGGAGAAGCAGCTGAGAGCAAAAGGGCTTGTGAGAAAAGCAGGGGAGGAGCTGAAGTGCTTTGGAGCAGAGTGCTTTAAGCTGTCGTTTATTGTGATTACGGGAGTTACTTTGTTGGGTATGCTCGTTTCGTTGATTTTAGTGATCAGAACGAGGAGTTTTTACAAGAGTGATATTTACAAGAAGTTCAGAGAGGAAGCTGAAACAGAGGTGGCCGGAAATGGCGCGGTGGTGGCCGCCGGAGCGCCGGAGGAGACAAGAAGGCAATAG

Protein sequence

MIGAGPGGGGGISRWSDIRTIAVHVITGRWFVVFASLLIMAANGGIFMFGLYSNDIKSVLGYDQTTLNLLSFFKDLGANVGVLSGLINEVSPPWVVLSSGAALNFFGYFMIWLAVTKRISTPKVWQMCVYICIGGNSQSFANTGSLVTCVKNFPESRGIVIGILKGYFGLSGAITTQLFHAFYGSDTKSLILLLGWLPAAISFAFLRTIRIMKVIRHPNELKVFYNFLYISLALAGFLMLMIIIESKLVLTQSAYGGSATVVLLLLFLPLAVVVIEEYNLWKLKTAAIGAPNPIQIITEKPNIPKTEHQKQTQQNQKQKQPSCWTTIFSPPERGEDFTILQALFSMDMLVLFFSATCGVGGTLTVIDNLGQIGMSLGYPKRSIGTFVTLVSIWNYLGRVASGFISEIVLTKYKFPRPLMLSLMLLFSCAGYLMVAFDVQNGLYAASIVIGFCFGAQWPLIYAIISELFGLKYYSTLFNFGSVASPIGLYVLNVKVKGNFYDREAEKQLRAKGLVRKAGEELKCFGAECFKLSFIVITGVTLLGMLVSLILVIRTRSFYKSDIYKKFREEAETEVAGNGAVVAAGAPEETRRQ

Homology

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BLAST of Lag0040471 vs. NCBI nr
Match: KAG6573851.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 991.1 bits (2561), Expect = 4.1e-285
Identity = 506/592 (85.47%), Postives = 542/592 (91.55%), Query Frame = 0


Query: 1   MIGAGPGGGGGISRWSDIRTIAVHVITGRWFVVFASLLIMAANGGIFMFGLYSNDIKSVL 60
           M+ AG GG G IS  S+IRTIAVH+ITGRWFVVFASLLIMAA G  +MF LYSNDIKSVL
Sbjct: 1   MVAAGQGGDGAISGSSNIRTIAVHIITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVL 60

Query: 61  GYDQTTLNLLSFFKDLGANVGVLSGLINEVSPPWVVLSSGAALNFFGYFMIWLAVTKRIS 120
           GYDQTTLNLLSFFKDLGANVG+LSGLINEV+PPWVVLS GA LNFFGYFMIWLAVTKRIS
Sbjct: 61  GYDQTTLNLLSFFKDLGANVGILSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRIS 120

Query: 121 TPKVWQMCVYICIGGNSQSFANTGSLVTCVKNFPESRGIVIGILKGYFGLSGAITTQLFH 180
           TPKVWQMC+YIC+G NSQSFANTGSLVTCVKNFP+SRG+V+GILKGY GLSGAI TQLFH
Sbjct: 121 TPKVWQMCLYICVGANSQSFANTGSLVTCVKNFPQSRGVVLGILKGYVGLSGAIITQLFH 180

Query: 181 AFYGSDTKSLILLLGWLPAAISFAFLRTIRIMKVIRHPNELKVFYNFLYISLALAGFLML 240
           AFYGSDTKSLILL+GWLPAAISFAFLRTIRIMKVIR PNELKVFYNFLYISLALAGFLML
Sbjct: 181 AFYGSDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLML 240

Query: 241 MIIIESKLVLTQSAYGGSATVVLLLLFLPLAVVVIEEYNLWKLKTAAIGAPNPIQIITEK 300
           MIIIESKL+ +Q+ +GGSA VVLLLLFLPLAVV++EEYNL KLK+AAI  PNP+QI+TEK
Sbjct: 241 MIIIESKLIFSQAEFGGSAAVVLLLLFLPLAVVIVEEYNLSKLKSAAINPPNPLQILTEK 300

Query: 301 PNIPKTEHQKQTQQNQKQKQPSCWTTIFSPPERGEDFTILQALFSMDMLVLFFSATCGVG 360
           PN PKTE QKQT+Q   QKQPSCWTTIFSPPERGEDFTILQALFS+DM +LFFSA CGVG
Sbjct: 301 PNPPKTEPQKQTRQ---QKQPSCWTTIFSPPERGEDFTILQALFSLDMAILFFSAICGVG 360

Query: 361 GTLTVIDNLGQIGMSLGYPKRSIGTFVTLVSIWNYLGRVASGFISEIVLTKYKFPRPLML 420
           GTLT IDNLGQIGMSLGYPKRSI TFVTLVSIWNYLGRVASGF+SEIVLTKYKFPRPLML
Sbjct: 361 GTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLML 420

Query: 421 SLMLLFSCAGYLMVAFDVQNGLYAASIVIGFCFGAQWPLIYAIISELFGLKYYSTLFNFG 480
           SL LL SC G+L++AF V NGLY ASIVIGFCFGAQWPL++AIISELFGLKYYSTL+NFG
Sbjct: 421 SLTLLLSCVGHLIIAFAVPNGLYVASIVIGFCFGAQWPLLFAIISELFGLKYYSTLYNFG 480

Query: 481 SVASPIGLYVLNVKVKGNFYDREAEKQLRAKGLVRKAGEELKCFGAECFKLSFIVITGVT 540
           SVASPIGLYVLNVKV GN YD+EA KQL AKGLVRK GEELKC+G ECFK SF+VITGVT
Sbjct: 481 SVASPIGLYVLNVKVAGNLYDKEAAKQLAAKGLVRKTGEELKCYGGECFKRSFLVITGVT 540

Query: 541 LLGMLVSLILVIRTRSFYKSDIYKKFREEAETEVAGNGAVVAAGAPEETRRQ 593
           LLGM VSLILVIRTR FYKSDIYKKFR+EAE EVAGNGAV AAG PEETR +
Sbjct: 541 LLGMFVSLILVIRTRGFYKSDIYKKFRDEAEAEVAGNGAVAAAGVPEETRNR 589

[Close]

BLAST of Lag0040471 vs. NCBI nr
Match: XP_022944943.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucurbita moschata])

HSP 1 Score: 987.6 bits (2552), Expect = 4.5e-284
Identity = 506/590 (85.76%), Postives = 539/590 (91.36%), Query Frame = 0


Query: 1   MIGAGPGGGGGISRWSDIRTIAVHVITGRWFVVFASLLIMAANGGIFMFGLYSNDIKSVL 60
           M+ AG GG   IS  S+IRTIAVH+ITGRWFVVFASLLIMAA G  +MF LYSNDIKSVL
Sbjct: 1   MVAAGQGGDRAISGSSNIRTIAVHIITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVL 60

Query: 61  GYDQTTLNLLSFFKDLGANVGVLSGLINEVSPPWVVLSSGAALNFFGYFMIWLAVTKRIS 120
           GYDQTTLNLLSFFKDLGANVGVLSGLINEV+PPWVVLS GA LNFFGYFMIWLAVTKRIS
Sbjct: 61  GYDQTTLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRIS 120

Query: 121 TPKVWQMCVYICIGGNSQSFANTGSLVTCVKNFPESRGIVIGILKGYFGLSGAITTQLFH 180
           TPKVWQMC+YIC+G NSQSF NTGSLVTCVKNFPESRG+V+GILKGY GLSGAI TQLFH
Sbjct: 121 TPKVWQMCLYICVGANSQSFVNTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFH 180

Query: 181 AFYGSDTKSLILLLGWLPAAISFAFLRTIRIMKVIRHPNELKVFYNFLYISLALAGFLML 240
           AFYGSDTKSLILL+GWLPAAISFAFLRTIRIMKVIR PNELKVFYNFLYISLALAGFLML
Sbjct: 181 AFYGSDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLML 240

Query: 241 MIIIESKLVLTQSAYGGSATVVLLLLFLPLAVVVIEEYNLWKLKTAAIGAPNPIQIITEK 300
           MIIIESKL+ +Q+ +GGSA VVLLLLFLPLAVV++EEYNL KLK+AAI  PNP+QI+TEK
Sbjct: 241 MIIIESKLIFSQAEFGGSAAVVLLLLFLPLAVVIVEEYNLSKLKSAAINPPNPLQILTEK 300

Query: 301 PNIPKTEHQKQTQQNQKQKQPSCWTTIFSPPERGEDFTILQALFSMDMLVLFFSATCGVG 360
           PN PKTE QKQT+Q   QKQPSCWTTIFSPPERGEDFTILQALFS+DM +LFFSA CGVG
Sbjct: 301 PNPPKTEPQKQTRQ---QKQPSCWTTIFSPPERGEDFTILQALFSLDMAILFFSAICGVG 360

Query: 361 GTLTVIDNLGQIGMSLGYPKRSIGTFVTLVSIWNYLGRVASGFISEIVLTKYKFPRPLML 420
           GTLT IDNLGQIGMSLGYPKRSI TFVTLVSIWNYLGRVASGF+SEIVLTKYKFPRPLML
Sbjct: 361 GTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLML 420

Query: 421 SLMLLFSCAGYLMVAFDVQNGLYAASIVIGFCFGAQWPLIYAIISELFGLKYYSTLFNFG 480
           SL LL SC G+L++AF V NGLY ASIVIGFCFGAQWPL++AIISELFGLKYYSTL+NFG
Sbjct: 421 SLTLLLSCVGHLIIAFAVPNGLYVASIVIGFCFGAQWPLLFAIISELFGLKYYSTLYNFG 480

Query: 481 SVASPIGLYVLNVKVKGNFYDREAEKQLRAKGLVRKAGEELKCFGAECFKLSFIVITGVT 540
           SVASPIGLYVLNVKV GN YD+EA KQL AKGLVRK GEELKC+G ECFK SF+VITGVT
Sbjct: 481 SVASPIGLYVLNVKVAGNLYDKEAAKQLAAKGLVRKTGEELKCYGGECFKRSFLVITGVT 540

Query: 541 LLGMLVSLILVIRTRSFYKSDIYKKFREEAETEVAGNGAVVAAGAPEETR 591
           LLGM VSLILVIRTR FYKSDIYKKFR+EAE EV GNGAV AAGAPEETR
Sbjct: 541 LLGMFVSLILVIRTRGFYKSDIYKKFRDEAEAEVVGNGAVTAAGAPEETR 587

[Close]

BLAST of Lag0040471 vs. NCBI nr
Match: XP_023541417.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 978.8 bits (2529), Expect = 2.1e-281
Identity = 501/590 (84.92%), Postives = 534/590 (90.51%), Query Frame = 0


Query: 1   MIGAGPGGGGGISRWSDIRTIAVHVITGRWFVVFASLLIMAANGGIFMFGLYSNDIKSVL 60
           M+  G GG G IS  S+IRT+AVH+ITGRWFVVFASLLIMAA G  +MF LYSNDIKSVL
Sbjct: 1   MVAPGQGGDGAISGGSNIRTLAVHIITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVL 60

Query: 61  GYDQTTLNLLSFFKDLGANVGVLSGLINEVSPPWVVLSSGAALNFFGYFMIWLAVTKRIS 120
           GYDQTTLNLLSFFKDLGANVGVLSGLINEV+PPWVVLS GA LNFFGYFMIWLAVTKRIS
Sbjct: 61  GYDQTTLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRIS 120

Query: 121 TPKVWQMCVYICIGGNSQSFANTGSLVTCVKNFPESRGIVIGILKGYFGLSGAITTQLFH 180
           TPKVWQMC+YICIG NSQSFANTGSLVTCVKNFPESRG+V+GILKGY GLSGAI TQLFH
Sbjct: 121 TPKVWQMCLYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFH 180

Query: 181 AFYGSDTKSLILLLGWLPAAISFAFLRTIRIMKVIRHPNELKVFYNFLYISLALAGFLML 240
           AFYGSDTKSLILL+GWLPAAISFAFLRTIRIMKVIR PNELKVFYNFLYISLALAGFLML
Sbjct: 181 AFYGSDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLML 240

Query: 241 MIIIESKLVLTQSAYGGSATVVLLLLFLPLAVVVIEEYNLWKLKTAAIGAPNPIQIITEK 300
           MIIIESKL+ TQ+ +GGSA VVLLLLFLPLAVV++EEYNL KLK+AAI  PNP+QI+TEK
Sbjct: 241 MIIIESKLIFTQAEFGGSAAVVLLLLFLPLAVVIVEEYNLSKLKSAAINPPNPLQILTEK 300

Query: 301 PNIPKTEHQKQTQQNQKQKQPSCWTTIFSPPERGEDFTILQALFSMDMLVLFFSATCGVG 360
           PN PKTE          +KQPSCWTTIF+PPERGEDFTILQALFS+DM +LFFSA CGVG
Sbjct: 301 PNPPKTE---------PRKQPSCWTTIFNPPERGEDFTILQALFSLDMAILFFSAICGVG 360

Query: 361 GTLTVIDNLGQIGMSLGYPKRSIGTFVTLVSIWNYLGRVASGFISEIVLTKYKFPRPLML 420
           GTLT IDNLGQIGMSLGYPKRSI TFVTLVSIWNYLGRVASGF+SEIVLTKYKFPRPLML
Sbjct: 361 GTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLML 420

Query: 421 SLMLLFSCAGYLMVAFDVQNGLYAASIVIGFCFGAQWPLIYAIISELFGLKYYSTLFNFG 480
           SL LL SC G+L++AF V NGLY ASIVIGFCFGAQWPL++AIISELFGLKYYSTL+NFG
Sbjct: 421 SLTLLLSCVGHLIIAFAVPNGLYVASIVIGFCFGAQWPLLFAIISELFGLKYYSTLYNFG 480

Query: 481 SVASPIGLYVLNVKVKGNFYDREAEKQLRAKGLVRKAGEELKCFGAECFKLSFIVITGVT 540
           SVASPIGLYVLNVKV GN YD+EA KQL AKGLVRK GEELKC+G ECFK SF+VITGVT
Sbjct: 481 SVASPIGLYVLNVKVAGNLYDKEAAKQLAAKGLVRKTGEELKCYGGECFKRSFLVITGVT 540

Query: 541 LLGMLVSLILVIRTRSFYKSDIYKKFREEAETEVAGNGAVVAAGAPEETR 591
           LLGM VSLILVIRTR FYKSDIYKKFR+EAE EV GNGAV AAGAPEETR
Sbjct: 541 LLGMFVSLILVIRTRGFYKSDIYKKFRDEAEAEVVGNGAVAAAGAPEETR 581

[Close]

BLAST of Lag0040471 vs. NCBI nr
Match: XP_022956604.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata])

HSP 1 Score: 978.4 bits (2528), Expect = 2.7e-281
Identity = 501/588 (85.20%), Postives = 539/588 (91.67%), Query Frame = 0


Query: 9   GGGISRWSDIRTIAVHVITGRWFVVFASLLIMAANGGIFMFGLYSNDIKSVLGYDQTTLN 68
           GGGIS  SDI +IAVHVITGRWFVVFASLLIMAA G  +MF LYS+DIKSVLGYDQTTLN
Sbjct: 4   GGGISGCSDISSIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSSDIKSVLGYDQTTLN 63

Query: 69  LLSFFKDLGANVGVLSGLINEVSPPWVVLSSGAALNFFGYFMIWLAVTKRISTPKVWQMC 128
           LLSFFKDLGANVGVLSGLINEV+PPWVVLS GA +NFFGYFMIWLAVT++I+ PKVWQMC
Sbjct: 64  LLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVMNFFGYFMIWLAVTRKIAAPKVWQMC 123

Query: 129 VYICIGGNSQSFANTGSLVTCVKNFPESRGIVIGILKGYFGLSGAITTQLFHAFYGSDTK 188
           +YICIG NSQSFANTGSLVTCVKNFPESRG+V+GILKGY GLSGAI TQLFHAFYG DTK
Sbjct: 124 LYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGGDTK 183

Query: 189 SLILLLGWLPAAISFAFLRTIRIMKVIRHPNELKVFYNFLYISLALAGFLMLMIIIESKL 248
           SLILL+GWLPAAISFAFLRTIRIMKVIR PNELKVFYNFLYISLALAGFLMLMII+ESK 
Sbjct: 184 SLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVESKT 243

Query: 249 VLTQSAYGGSATVVLLLLFLPLAVVVIEEYNLWKLK----TAAIGAPN-PIQIITEKPNI 308
             +QS YGGSA VVLLLLFLPLAVV+IEEYNLW LK    TAAI +PN P+QI+TEK  +
Sbjct: 244 EFSQSGYGGSAAVVLLLLFLPLAVVIIEEYNLWNLKTAAATAAIRSPNHPVQIVTEK--L 303

Query: 309 PKTEHQKQTQQNQKQKQPSCWTTIFSPPERGEDFTILQALFSMDMLVLFFSATCGVGGTL 368
           PKTEHQK   + + QK+PSCWTTIF+PPERGEDFTILQALFS+DML+LF +A CGVGGTL
Sbjct: 304 PKTEHQKHIPKTEHQKEPSCWTTIFNPPERGEDFTILQALFSVDMLILFLAAICGVGGTL 363

Query: 369 TVIDNLGQIGMSLGYPKRSIGTFVTLVSIWNYLGRVASGFISEIVLTKYKFPRPLMLSLM 428
           T IDNLGQIGMSLGYPK+SI TFVTLVSIWNYLGRV SGF+SEIVLTKYKFPRPLMLS+ 
Sbjct: 364 TAIDNLGQIGMSLGYPKQSISTFVTLVSIWNYLGRVTSGFVSEIVLTKYKFPRPLMLSMT 423

Query: 429 LLFSCAGYLMVAFDVQNGLYAASIVIGFCFGAQWPLIYAIISELFGLKYYSTLFNFGSVA 488
           LL SC G+LM+AFDV NGLY ASIVIGFCFGAQWPLI+AIISELFGLKYYSTL+NFGSVA
Sbjct: 424 LLVSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVA 483

Query: 489 SPIGLYVLNVKVKGNFYDREAEKQLRAKGLVRKAGEELKCFGAECFKLSFIVITGVTLLG 548
           SPIGLYVLNVKV GNFYDREAEKQLRAKG+VRK GEELKCFG ECFKLSF+VITGVTLLG
Sbjct: 484 SPIGLYVLNVKVAGNFYDREAEKQLRAKGIVRKTGEELKCFGGECFKLSFLVITGVTLLG 543

Query: 549 MLVSLILVIRTRSFYKSDIYKKFREEAETEVAGNGAVVAAGAPEETRR 592
           M VSLILVIRTRSFYKSDIYKKFR+EAETE+AGNGAVVAAG PEETR+
Sbjct: 544 MFVSLILVIRTRSFYKSDIYKKFRDEAETEMAGNGAVVAAGPPEETRK 589

[Close]

BLAST of Lag0040471 vs. NCBI nr
Match: KAG6601185.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 978.4 bits (2528), Expect = 2.7e-281
Identity = 500/588 (85.03%), Postives = 540/588 (91.84%), Query Frame = 0


Query: 9   GGGISRWSDIRTIAVHVITGRWFVVFASLLIMAANGGIFMFGLYSNDIKSVLGYDQTTLN 68
           GGGI+  SDI +IAVH+ITGRWFVVFASLLIMAA G  +MFGLYS+DIKSVLGYDQTTLN
Sbjct: 4   GGGIAGCSDISSIAVHIITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQTTLN 63

Query: 69  LLSFFKDLGANVGVLSGLINEVSPPWVVLSSGAALNFFGYFMIWLAVTKRISTPKVWQMC 128
           LLSFFKDLGANVGVLSGLINEV+PPWVVLS GA +NFFGYFMIWLAVT++I+ PKVWQMC
Sbjct: 64  LLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAIMNFFGYFMIWLAVTQKIAAPKVWQMC 123

Query: 129 VYICIGGNSQSFANTGSLVTCVKNFPESRGIVIGILKGYFGLSGAITTQLFHAFYGSDTK 188
           +YICIG NSQSFANTGSLVTCVKNFPESRG+V+GILKGY GLSGAI TQLFHAFYG DTK
Sbjct: 124 LYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGGDTK 183

Query: 189 SLILLLGWLPAAISFAFLRTIRIMKVIRHPNELKVFYNFLYISLALAGFLMLMIIIESKL 248
           SLILL+GWLPAAISFAFLRTIRIMKVIR PNELKVFYNFLYISLALAGFLMLMII+ESK 
Sbjct: 184 SLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVESKT 243

Query: 249 VLTQSAYGGSATVVLLLLFLPLAVVVIEEYNLWKLK----TAAIGAPN-PIQIITEKPNI 308
             +QS YGGSA VVLLLLFLPLAVV+IEEYNLW LK    TAAI +PN P+QI+TEK  +
Sbjct: 244 EFSQSGYGGSAAVVLLLLFLPLAVVIIEEYNLWNLKTAAATAAIRSPNHPVQIVTEK--L 303

Query: 309 PKTEHQKQTQQNQKQKQPSCWTTIFSPPERGEDFTILQALFSMDMLVLFFSATCGVGGTL 368
           PKTEHQK   + + QK+PSCWTTIF+PPERGEDFTILQALFS+DML+LF +A CGVGGTL
Sbjct: 304 PKTEHQKHIPKTEHQKEPSCWTTIFNPPERGEDFTILQALFSVDMLILFLAAICGVGGTL 363

Query: 369 TVIDNLGQIGMSLGYPKRSIGTFVTLVSIWNYLGRVASGFISEIVLTKYKFPRPLMLSLM 428
           T IDNLGQIGMSLGYPK+SI TFVTLVSIWNYLGRV SGF+SEIVLTKYKFPRPLMLS+ 
Sbjct: 364 TAIDNLGQIGMSLGYPKQSISTFVTLVSIWNYLGRVTSGFVSEIVLTKYKFPRPLMLSMT 423

Query: 429 LLFSCAGYLMVAFDVQNGLYAASIVIGFCFGAQWPLIYAIISELFGLKYYSTLFNFGSVA 488
           LL SC G+LM+AFDV NGLY ASIVIGFCFGAQWPLI+AIISELFGLKYYSTL+NFGSVA
Sbjct: 424 LLVSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVA 483

Query: 489 SPIGLYVLNVKVKGNFYDREAEKQLRAKGLVRKAGEELKCFGAECFKLSFIVITGVTLLG 548
           SPIGLYVLNVKV GNFYDREAEKQLRAKG+VRK GEELKCFG ECFKLSF+VITGVTLLG
Sbjct: 484 SPIGLYVLNVKVAGNFYDREAEKQLRAKGIVRKTGEELKCFGGECFKLSFLVITGVTLLG 543

Query: 549 MLVSLILVIRTRSFYKSDIYKKFREEAETEVAGNGAVVAAGAPEETRR 592
           M VSLILVIRTRSFYKSDIYKKFR+EAETE+AGNGAVVAAG PEETR+
Sbjct: 544 MFVSLILVIRTRSFYKSDIYKKFRDEAETEMAGNGAVVAAGPPEETRK 589

[Close]

BLAST of Lag0040471 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 1.6e-29
Identity = 135/543 (24.86%), Postives = 246/543 (45.30%), Query Frame = 0


Query: 29  RWFVVFASLLIMAANGGIFMFGLYSNDIKSVLGYDQTTLNLLSFFKDLGANVGVLSGLIN 88
           +W V+ A++ I A+ G  F F  YS+ +KSVLG  Q  LN L+   DLG   G  SG+  
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 89  EVSPPWVVLSSGAALNFFGYFMIWLAVTKRISTPKVWQMCVYIC--IGGNSQSFANTGSL 148
              P  VVL + AA+ F GY + WL +T  I+ P      V++C  + G S  + NT   
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP---YSLVFLCCLLAGLSICWFNTACF 162

Query: 149 VTCVKNFPESRGIVIGILKGYFGLSGAITTQLFHAFYGSDTKSLILLLGWLPAAISFAFL 208
           + C+++FP +R + + +   + G+S A+ +  F+A   S +   +LL   +P  +SFA L
Sbjct: 163 ILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLVVSFAAL 222

Query: 209 RTIRIMKVI---------RHPNELKVFYNFLYISLALAGFLMLMIIIESKLVLTQSAYGG 268
             +     +         RH + +    N L +   +  F +L+    S    + +    
Sbjct: 223 YPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAV---ITSFHLLL----SSSSTSSARLNF 282

Query: 269 SATVVLLLLFLPLAVVVIEEYNLWKLKTAAIGAPNPIQIITEKPNIPKTEHQKQTQQNQK 328
              VVLL+  L   ++V        +  A +   +   ++    NI + ++QK +  ++ 
Sbjct: 283 IGAVVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVML---NIDELKNQKTSVSSKT 342

Query: 329 QKQPSCWTTIFSPPERGEDFTILQALFSMDMLVLFFSATCGVGGTLTVIDNLGQIGMSLG 388
             +        +    G++ +    +  ++  + + +  CG    L   +NLGQI  SLG
Sbjct: 343 GYEHMGTAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLG 402

Query: 389 YPKRSIGTFVTLVSIWNYLGRVASGFISEIVLTKYKFPRPLMLSLMLLFSCAGY--LMVA 448
              ++  T VT+ S +++ GR+ S    + +  +++  R    ++ LL +   +  L V+
Sbjct: 403 ---QNSTTLVTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVS 462

Query: 449 FDVQNGLYAASIVIGFCFGAQWPLIYAIISELFGLKYYSTLFNFGSVASPIGLYVLNVKV 508
              Q  L  A+ +IG   G  +    +I S+LFG        N      PIG  +L   +
Sbjct: 463 SSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIG-SLLYGYI 522

Query: 509 KGNFYDREAEKQLRAKGLVRKAGEELKCFGAECFKLSFIVITGVTLLGMLVSLILVIRTR 559
             + Y+  A     +  +     + + C G +C+  +F+    +++LG++ SL L IRT+
Sbjct: 523 AASIYEANA-----SPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTK 562

[Close]

BLAST of Lag0040471 vs. ExPASy Swiss-Prot
Match: Q59MJ2 (Probable transporter MCH1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 1.2e-05
Identity = 49/178 (27.53%), Postives = 76/178 (42.70%), Query Frame = 0


Query: 36  SLLIMAANGGIFMFGLYSNDIKSVLGYDQTTLNLLSFFKDLGAN--VGVLSGLINEVSPP 95
           SLL     G I +F LY++    VLG     +N++S    LG    + VL  L +   P 
Sbjct: 33  SLLSCLVAGSILLFTLYTSSFHEVLGLSYLQINMISSLSALGMYFCLPVLGYLADSYGPA 92

Query: 96  WVVLSSGAALNFF--GYFMIWLAVTKRISTPKVWQMCVYICIGGNSQSFANTGSLVTCVK 155
            + L S   + FF   YF+    V+ +  +  V   CV  C  G + S     SL+TC +
Sbjct: 93  LLSLFS---IWFFCPSYFVNSYLVSTQ--SGSVIGFCVCFCFIGLATSSLYFSSLITCAR 152

Query: 156 NFPESRGIVIGILKGYFGLSGAITTQL-----FHAFYGSDTKSLILLLGWLPAAISFA 205
             P+ +G+ I +    +GLS  +  Q+     FH     D + +     WL   +  A
Sbjct: 153 ICPDHKGLAISLPITCYGLSALLGAQILKLPYFHRHDVLDLEVVFSFFAWLYLVVGIA 205

[Close]

BLAST of Lag0040471 vs. ExPASy TrEMBL
Match: A0A6J1FZF2 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucurbita moschata OX=3662 GN=LOC111449326 PE=4 SV=1)

HSP 1 Score: 987.6 bits (2552), Expect = 2.2e-284
Identity = 506/590 (85.76%), Postives = 539/590 (91.36%), Query Frame = 0


Query: 1   MIGAGPGGGGGISRWSDIRTIAVHVITGRWFVVFASLLIMAANGGIFMFGLYSNDIKSVL 60
           M+ AG GG   IS  S+IRTIAVH+ITGRWFVVFASLLIMAA G  +MF LYSNDIKSVL
Sbjct: 1   MVAAGQGGDRAISGSSNIRTIAVHIITGRWFVVFASLLIMAAAGATYMFSLYSNDIKSVL 60

Query: 61  GYDQTTLNLLSFFKDLGANVGVLSGLINEVSPPWVVLSSGAALNFFGYFMIWLAVTKRIS 120
           GYDQTTLNLLSFFKDLGANVGVLSGLINEV+PPWVVLS GA LNFFGYFMIWLAVTKRIS
Sbjct: 61  GYDQTTLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTKRIS 120

Query: 121 TPKVWQMCVYICIGGNSQSFANTGSLVTCVKNFPESRGIVIGILKGYFGLSGAITTQLFH 180
           TPKVWQMC+YIC+G NSQSF NTGSLVTCVKNFPESRG+V+GILKGY GLSGAI TQLFH
Sbjct: 121 TPKVWQMCLYICVGANSQSFVNTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFH 180

Query: 181 AFYGSDTKSLILLLGWLPAAISFAFLRTIRIMKVIRHPNELKVFYNFLYISLALAGFLML 240
           AFYGSDTKSLILL+GWLPAAISFAFLRTIRIMKVIR PNELKVFYNFLYISLALAGFLML
Sbjct: 181 AFYGSDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLML 240

Query: 241 MIIIESKLVLTQSAYGGSATVVLLLLFLPLAVVVIEEYNLWKLKTAAIGAPNPIQIITEK 300
           MIIIESKL+ +Q+ +GGSA VVLLLLFLPLAVV++EEYNL KLK+AAI  PNP+QI+TEK
Sbjct: 241 MIIIESKLIFSQAEFGGSAAVVLLLLFLPLAVVIVEEYNLSKLKSAAINPPNPLQILTEK 300

Query: 301 PNIPKTEHQKQTQQNQKQKQPSCWTTIFSPPERGEDFTILQALFSMDMLVLFFSATCGVG 360
           PN PKTE QKQT+Q   QKQPSCWTTIFSPPERGEDFTILQALFS+DM +LFFSA CGVG
Sbjct: 301 PNPPKTEPQKQTRQ---QKQPSCWTTIFSPPERGEDFTILQALFSLDMAILFFSAICGVG 360

Query: 361 GTLTVIDNLGQIGMSLGYPKRSIGTFVTLVSIWNYLGRVASGFISEIVLTKYKFPRPLML 420
           GTLT IDNLGQIGMSLGYPKRSI TFVTLVSIWNYLGRVASGF+SEIVLTKYKFPRPLML
Sbjct: 361 GTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFVSEIVLTKYKFPRPLML 420

Query: 421 SLMLLFSCAGYLMVAFDVQNGLYAASIVIGFCFGAQWPLIYAIISELFGLKYYSTLFNFG 480
           SL LL SC G+L++AF V NGLY ASIVIGFCFGAQWPL++AIISELFGLKYYSTL+NFG
Sbjct: 421 SLTLLLSCVGHLIIAFAVPNGLYVASIVIGFCFGAQWPLLFAIISELFGLKYYSTLYNFG 480

Query: 481 SVASPIGLYVLNVKVKGNFYDREAEKQLRAKGLVRKAGEELKCFGAECFKLSFIVITGVT 540
           SVASPIGLYVLNVKV GN YD+EA KQL AKGLVRK GEELKC+G ECFK SF+VITGVT
Sbjct: 481 SVASPIGLYVLNVKVAGNLYDKEAAKQLAAKGLVRKTGEELKCYGGECFKRSFLVITGVT 540

Query: 541 LLGMLVSLILVIRTRSFYKSDIYKKFREEAETEVAGNGAVVAAGAPEETR 591
           LLGM VSLILVIRTR FYKSDIYKKFR+EAE EV GNGAV AAGAPEETR
Sbjct: 541 LLGMFVSLILVIRTRGFYKSDIYKKFRDEAEAEVVGNGAVTAAGAPEETR 587

[Close]

BLAST of Lag0040471 vs. ExPASy TrEMBL
Match: A0A6J1GZI8 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111458291 PE=4 SV=1)

HSP 1 Score: 978.4 bits (2528), Expect = 1.3e-281
Identity = 501/588 (85.20%), Postives = 539/588 (91.67%), Query Frame = 0


Query: 9   GGGISRWSDIRTIAVHVITGRWFVVFASLLIMAANGGIFMFGLYSNDIKSVLGYDQTTLN 68
           GGGIS  SDI +IAVHVITGRWFVVFASLLIMAA G  +MF LYS+DIKSVLGYDQTTLN
Sbjct: 4   GGGISGCSDISSIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSSDIKSVLGYDQTTLN 63

Query: 69  LLSFFKDLGANVGVLSGLINEVSPPWVVLSSGAALNFFGYFMIWLAVTKRISTPKVWQMC 128
           LLSFFKDLGANVGVLSGLINEV+PPWVVLS GA +NFFGYFMIWLAVT++I+ PKVWQMC
Sbjct: 64  LLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVMNFFGYFMIWLAVTRKIAAPKVWQMC 123

Query: 129 VYICIGGNSQSFANTGSLVTCVKNFPESRGIVIGILKGYFGLSGAITTQLFHAFYGSDTK 188
           +YICIG NSQSFANTGSLVTCVKNFPESRG+V+GILKGY GLSGAI TQLFHAFYG DTK
Sbjct: 124 LYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGGDTK 183

Query: 189 SLILLLGWLPAAISFAFLRTIRIMKVIRHPNELKVFYNFLYISLALAGFLMLMIIIESKL 248
           SLILL+GWLPAAISFAFLRTIRIMKVIR PNELKVFYNFLYISLALAGFLMLMII+ESK 
Sbjct: 184 SLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVESKT 243

Query: 249 VLTQSAYGGSATVVLLLLFLPLAVVVIEEYNLWKLK----TAAIGAPN-PIQIITEKPNI 308
             +QS YGGSA VVLLLLFLPLAVV+IEEYNLW LK    TAAI +PN P+QI+TEK  +
Sbjct: 244 EFSQSGYGGSAAVVLLLLFLPLAVVIIEEYNLWNLKTAAATAAIRSPNHPVQIVTEK--L 303

Query: 309 PKTEHQKQTQQNQKQKQPSCWTTIFSPPERGEDFTILQALFSMDMLVLFFSATCGVGGTL 368
           PKTEHQK   + + QK+PSCWTTIF+PPERGEDFTILQALFS+DML+LF +A CGVGGTL
Sbjct: 304 PKTEHQKHIPKTEHQKEPSCWTTIFNPPERGEDFTILQALFSVDMLILFLAAICGVGGTL 363

Query: 369 TVIDNLGQIGMSLGYPKRSIGTFVTLVSIWNYLGRVASGFISEIVLTKYKFPRPLMLSLM 428
           T IDNLGQIGMSLGYPK+SI TFVTLVSIWNYLGRV SGF+SEIVLTKYKFPRPLMLS+ 
Sbjct: 364 TAIDNLGQIGMSLGYPKQSISTFVTLVSIWNYLGRVTSGFVSEIVLTKYKFPRPLMLSMT 423

Query: 429 LLFSCAGYLMVAFDVQNGLYAASIVIGFCFGAQWPLIYAIISELFGLKYYSTLFNFGSVA 488
           LL SC G+LM+AFDV NGLY ASIVIGFCFGAQWPLI+AIISELFGLKYYSTL+NFGSVA
Sbjct: 424 LLVSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVA 483

Query: 489 SPIGLYVLNVKVKGNFYDREAEKQLRAKGLVRKAGEELKCFGAECFKLSFIVITGVTLLG 548
           SPIGLYVLNVKV GNFYDREAEKQLRAKG+VRK GEELKCFG ECFKLSF+VITGVTLLG
Sbjct: 484 SPIGLYVLNVKVAGNFYDREAEKQLRAKGIVRKTGEELKCFGGECFKLSFLVITGVTLLG 543

Query: 549 MLVSLILVIRTRSFYKSDIYKKFREEAETEVAGNGAVVAAGAPEETRR 592
           M VSLILVIRTRSFYKSDIYKKFR+EAETE+AGNGAVVAAG PEETR+
Sbjct: 544 MFVSLILVIRTRSFYKSDIYKKFRDEAETEMAGNGAVVAAGPPEETRK 589

[Close]

BLAST of Lag0040471 vs. ExPASy TrEMBL
Match: A0A6J1K186 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111489721 PE=4 SV=1)

HSP 1 Score: 966.5 bits (2497), Expect = 5.2e-278
Identity = 500/589 (84.89%), Postives = 532/589 (90.32%), Query Frame = 0


Query: 9   GGGISRWSDIRTIAVHVITGRWFVVFASLLIMAANGGIFMFGLYSNDIKSVLGYDQTTLN 68
           GGGIS  SDI +IAVHVITGRWFVVFASLLIMAA G  +MF LYS+DIKSVLGYDQTTLN
Sbjct: 4   GGGISGCSDISSIAVHVITGRWFVVFASLLIMAAAGATYMFSLYSSDIKSVLGYDQTTLN 63

Query: 69  LLSFFKDLGANVGVLSGLINEVSPPWVVLSSGAALNFFGYFMIWLAVTKRISTPKVWQMC 128
           LLSFFKDLGANVGVLSGLINEV+PPWVVLS GA +NFFGYFMIWLAVT +IS PKVWQMC
Sbjct: 64  LLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVMNFFGYFMIWLAVTGKISAPKVWQMC 123

Query: 129 VYICIGGNSQSFANTGSLVTCVKNFPESRGIVIGILKGYFGLSGAITTQLFHAFYGSDTK 188
           +YICIG NSQSFANTGSLVTCVKNFPESRG+V+GILKGY GLSGAI TQLFHAFYG DTK
Sbjct: 124 LYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGGDTK 183

Query: 189 SLILLLGWLPAAISFAFLRTIRIMKVIRHPNELKVFYNFLYISLALAGFLMLMIIIESKL 248
           SLILL+GWLPAAISFAFLRTIRIMKVIR PNELKVFYNFLYISLALAGFLMLMIIIESK 
Sbjct: 184 SLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIIESKT 243

Query: 249 VLTQSAYGGSATVVLLLLFLPLAVVVIEEYNLWKLKT-----AAIGAPN-PIQIITEKPN 308
             +QS YGGSA VVLLLLFLPLAVV+IEEYNLW LKT     AAI +PN P+QI+TEK  
Sbjct: 244 EFSQSGYGGSAAVVLLLLFLPLAVVIIEEYNLWNLKTAAATAAAIRSPNHPVQIVTEK-- 303

Query: 309 IPKTEHQKQTQQNQKQKQPSCWTTIFSPPERGEDFTILQALFSMDMLVLFFSATCGVGGT 368
           +PKTEH         QKQPSCWTTIF+PPERGEDFTILQALFS+DM +LF +A CGVGGT
Sbjct: 304 VPKTEH---------QKQPSCWTTIFNPPERGEDFTILQALFSVDMFILFLAAICGVGGT 363

Query: 369 LTVIDNLGQIGMSLGYPKRSIGTFVTLVSIWNYLGRVASGFISEIVLTKYKFPRPLMLSL 428
           LT IDNLGQIGMSLGYPK+SI TFVTLVSIWNYLGRV SGF+SEIVLTKYKFPRPLMLS+
Sbjct: 364 LTAIDNLGQIGMSLGYPKQSISTFVTLVSIWNYLGRVTSGFVSEIVLTKYKFPRPLMLSM 423

Query: 429 MLLFSCAGYLMVAFDVQNGLYAASIVIGFCFGAQWPLIYAIISELFGLKYYSTLFNFGSV 488
            LL SC G+LM+AFDV NGLY ASIVIGFCFGAQWPLI+AIISELFGLKYYSTL+NFGSV
Sbjct: 424 TLLVSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSV 483

Query: 489 ASPIGLYVLNVKVKGNFYDREAEKQLRAKGLVRKAGEELKCFGAECFKLSFIVITGVTLL 548
           ASPIGLYVLNVKV GNFYDREAEKQLRAKG+VRK GEELKCFG ECFKLSF+VITGVTLL
Sbjct: 484 ASPIGLYVLNVKVAGNFYDREAEKQLRAKGIVRKTGEELKCFGGECFKLSFLVITGVTLL 543

Query: 549 GMLVSLILVIRTRSFYKSDIYKKFREEAETEVAGNGAVVAAGAPEETRR 592
           GM VSLILVIRTRSFYKSDIYKKFR+EAETE+AGNGAVVAA  PEETR+
Sbjct: 544 GMFVSLILVIRTRSFYKSDIYKKFRDEAETEIAGNGAVVAAEPPEETRK 581

[Close]

BLAST of Lag0040471 vs. ExPASy TrEMBL
Match: A0A5D3CFW3 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002400 PE=4 SV=1)

HSP 1 Score: 964.5 bits (2492), Expect = 2.0e-277
Identity = 496/585 (84.79%), Postives = 534/585 (91.28%), Query Frame = 0


Query: 9   GGGISRWSDIRTIAVHVITGRWFVVFASLLIMAANGGIFMFGLYSNDIKSVLGYDQTTLN 68
           GGGIS  SDI +IAVHVITGRWFVVFASLLIMAA G  +MFGLYS+DIKSVLGYDQTTLN
Sbjct: 4   GGGISGCSDIGSIAVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQTTLN 63

Query: 69  LLSFFKDLGANVGVLSGLINEVSPPWVVLSSGAALNFFGYFMIWLAVTKRISTPKVWQMC 128
           LLSFFKDLGANVGVLSGLINEV+PPWVVLS GA LNFFGYFMIWLAVT+RISTPKVWQMC
Sbjct: 64  LLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISTPKVWQMC 123

Query: 129 VYICIGGNSQSFANTGSLVTCVKNFPESRGIVIGILKGYFGLSGAITTQLFHAFYGSDTK 188
           +YICIG NSQSFANTGSLVTCVKNFPESRG+V+GILKGY GLSGAI TQLFHAFYG+DTK
Sbjct: 124 LYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGNDTK 183

Query: 189 SLILLLGWLPAAISFAFLRTIRIMKVIRHPNELKVFYNFLYISLALAGFLMLMIIIESKL 248
           SLILL+GWLPAAISFA LRTIRIMKVIR PNELKVFYNFLYISLALAGFLMLMII+ESK 
Sbjct: 184 SLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVESKK 243

Query: 249 VLTQSAYGGSATVVLLLLFLPLAVVVIEEYNLWKLKTAAIGAPNP-IQIITEKPNIPKTE 308
              Q+ YGGSA VVLLLLFLPL+VV+IEEYNLWKLKTA + +PNP +QI+TEK  +PKTE
Sbjct: 244 EFNQNEYGGSAAVVLLLLFLPLSVVIIEEYNLWKLKTALVKSPNPSVQIVTEK--LPKTE 303

Query: 309 HQKQTQQNQKQKQPSCWTTIFSPPERGEDFTILQALFSMDMLVLFFSATCGVGGTLTVID 368
           H KQ +    QK+PSCWTTIF+PP+RGEDFTILQALFS DML+LF +A CGVGGTLT ID
Sbjct: 304 HPKQLE----QKEPSCWTTIFNPPQRGEDFTILQALFSFDMLILFIAAICGVGGTLTAID 363

Query: 369 NLGQIGMSLGYPKRSIGTFVTLVSIWNYLGRVASGFISEIVLTKYKFPRPLMLSLMLLFS 428
           NLGQIGMSLGYPKRSI TFVTLVSIWNYLGRVASGFISEIVLTKYKFPRPL+LSL LL S
Sbjct: 364 NLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLS 423

Query: 429 CAGYLMVAFDVQNGLYAASIVIGFCFGAQWPLIYAIISELFGLKYYSTLFNFGSVASPIG 488
           C G+L++AFDV NGLY ASIVIGFCFGAQWPLI+AIISELFGLKYYSTL+NFGSVASPIG
Sbjct: 424 CVGHLIIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIG 483

Query: 489 LYVLNVKVKGNFYDREAEKQLRAKGLVRKAGEELKCFGAECFKLSFIVITGVTLLGMLVS 548
           LY+LNVKV GNFYDREAEKQL AKG++RKAGEELKCFG ECFKLSFIVIT VTLLGMLVS
Sbjct: 484 LYILNVKVAGNFYDREAEKQLSAKGIIRKAGEELKCFGGECFKLSFIVITAVTLLGMLVS 543

Query: 549 LILVIRTRSFYKSDIYKKFREEAET-EVAGNGAVVAAGAPEETRR 592
           L+LVIRTRSFYKSDIYKKFR+EAET EVAGN  +  AG PEE R+
Sbjct: 544 LVLVIRTRSFYKSDIYKKFRDEAETKEVAGNAVIEVAGVPEERRK 582

[Close]

BLAST of Lag0040471 vs. ExPASy TrEMBL
Match: A0A1S3BG09 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103489435 PE=4 SV=1)

HSP 1 Score: 964.5 bits (2492), Expect = 2.0e-277
Identity = 496/585 (84.79%), Postives = 534/585 (91.28%), Query Frame = 0


Query: 9   GGGISRWSDIRTIAVHVITGRWFVVFASLLIMAANGGIFMFGLYSNDIKSVLGYDQTTLN 68
           GGGIS  SDI +IAVHVITGRWFVVFASLLIMAA G  +MFGLYS+DIKSVLGYDQTTLN
Sbjct: 4   GGGISGCSDIGSIAVHVITGRWFVVFASLLIMAAAGATYMFGLYSSDIKSVLGYDQTTLN 63

Query: 69  LLSFFKDLGANVGVLSGLINEVSPPWVVLSSGAALNFFGYFMIWLAVTKRISTPKVWQMC 128
           LLSFFKDLGANVGVLSGLINEV+PPWVVLS GA LNFFGYFMIWLAVT+RISTPKVWQMC
Sbjct: 64  LLSFFKDLGANVGVLSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAVTRRISTPKVWQMC 123

Query: 129 VYICIGGNSQSFANTGSLVTCVKNFPESRGIVIGILKGYFGLSGAITTQLFHAFYGSDTK 188
           +YICIG NSQSFANTGSLVTCVKNFPESRG+V+GILKGY GLSGAI TQLFHAFYG+DTK
Sbjct: 124 LYICIGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGNDTK 183

Query: 189 SLILLLGWLPAAISFAFLRTIRIMKVIRHPNELKVFYNFLYISLALAGFLMLMIIIESKL 248
           SLILL+GWLPAAISFA LRTIRIMKVIR PNELKVFYNFLYISLALAGFLMLMII+ESK 
Sbjct: 184 SLILLIGWLPAAISFASLRTIRIMKVIRQPNELKVFYNFLYISLALAGFLMLMIIVESKK 243

Query: 249 VLTQSAYGGSATVVLLLLFLPLAVVVIEEYNLWKLKTAAIGAPNP-IQIITEKPNIPKTE 308
              Q+ YGGSA VVLLLLFLPL+VV+IEEYNLWKLKTA + +PNP +QI+TEK  +PKTE
Sbjct: 244 EFNQNEYGGSAAVVLLLLFLPLSVVIIEEYNLWKLKTALVKSPNPSVQIVTEK--LPKTE 303

Query: 309 HQKQTQQNQKQKQPSCWTTIFSPPERGEDFTILQALFSMDMLVLFFSATCGVGGTLTVID 368
           H KQ +    QK+PSCWTTIF+PP+RGEDFTILQALFS DML+LF +A CGVGGTLT ID
Sbjct: 304 HPKQLE----QKEPSCWTTIFNPPQRGEDFTILQALFSFDMLILFIAAICGVGGTLTAID 363

Query: 369 NLGQIGMSLGYPKRSIGTFVTLVSIWNYLGRVASGFISEIVLTKYKFPRPLMLSLMLLFS 428
           NLGQIGMSLGYPKRSI TFVTLVSIWNYLGRVASGFISEIVLTKYKFPRPL+LSL LL S
Sbjct: 364 NLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFISEIVLTKYKFPRPLILSLTLLLS 423

Query: 429 CAGYLMVAFDVQNGLYAASIVIGFCFGAQWPLIYAIISELFGLKYYSTLFNFGSVASPIG 488
           C G+L++AFDV NGLY ASIVIGFCFGAQWPLI+AIISELFGLKYYSTL+NFGSVASPIG
Sbjct: 424 CVGHLIIAFDVPNGLYVASIVIGFCFGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIG 483

Query: 489 LYVLNVKVKGNFYDREAEKQLRAKGLVRKAGEELKCFGAECFKLSFIVITGVTLLGMLVS 548
           LY+LNVKV GNFYDREAEKQL AKG++RKAGEELKCFG ECFKLSFIVIT VTLLGMLVS
Sbjct: 484 LYILNVKVAGNFYDREAEKQLSAKGIIRKAGEELKCFGGECFKLSFIVITAVTLLGMLVS 543

Query: 549 LILVIRTRSFYKSDIYKKFREEAET-EVAGNGAVVAAGAPEETRR 592
           L+LVIRTRSFYKSDIYKKFR+EAET EVAGN  +  AG PEE R+
Sbjct: 544 LVLVIRTRSFYKSDIYKKFRDEAETKEVAGNAVIEVAGVPEERRK 582

[Close]

BLAST of Lag0040471 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 788.5 bits (2035), Expect = 3.8e-228
Identity = 395/596 (66.28%), Postives = 474/596 (79.53%), Query Frame = 0


Query: 1   MIGAGPGGGGGISRWSDIRTIAVHVITGRWFVVFASLLIMAANGGIFMFGLYSNDIKSVL 60
           M+ A PGG         ++++ + ++TGRWF+ F SLLIM+  G  +MFG+YS DIK  L
Sbjct: 1   MVAASPGG--------SMKSLTIQILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETL 60

Query: 61  GYDQTTLNLLSFFKDLGANVGVLSGLINEVSPPWVVLSSGAALNFFGYFMIWLAVTKRIS 120
           GYDQTTLNLLSFFKDLGANVGVL+GL+NEV+PPW +L  GA LNFFGYFMIWLAVT+RIS
Sbjct: 61  GYDQTTLNLLSFFKDLGANVGVLAGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERIS 120

Query: 121 TPKVWQMCVYICIGGNSQSFANTGSLVTCVKNFPESRGIVIGILKGYFGLSGAITTQLFH 180
            P+VW MC+YIC+G NSQSFANTGSLVTCVKNFPESRG+V+GILKGY GLSGAI TQL+ 
Sbjct: 121 KPQVWHMCLYICVGANSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYR 180

Query: 181 AFYGSDTKSLILLLGWLPAAISFAFLRTIRIMKVIRHPNELKVFYNFLYISLALAGFLML 240
           AFYG DTK LIL++GWLPA +SFAFLRTIRIMKV R  NELKVFYNFLYISL LA FLM+
Sbjct: 181 AFYGEDTKELILMIGWLPAIVSFAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMV 240

Query: 241 MIIIESKLVLTQSAYGGSATVVLLLLFLPLAVVVIEEYNLWKLKTAAIGAPNPIQIITEK 300
           +III      TQS +GGSA VV++LL LP+ VV++EE  LWK K  A+  P PI ++TEK
Sbjct: 241 VIIINKLSGFTQSEFGGSAAVVIVLLLLPIIVVILEEKKLWKEKQVALNDPAPINVVTEK 300

Query: 301 PNIPKTEHQKQTQQNQKQ-----KQPSCWTTIFSPPERGEDFTILQALFSMDMLVLFFSA 360
           P +  +E +    +  K+     K PSCWTT+F+PPERG+D+TILQALFS+DML+LF + 
Sbjct: 301 PKLDSSEFKDDDGEESKEVVEKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLAT 360

Query: 361 TCGVGGTLTVIDNLGQIGMSLGYPKRSIGTFVTLVSIWNYLGRVASGFISEIVLTKYKFP 420
            CGVGGTLT IDNLGQIG SLGYPKRS+ TFV+LVSIWNY GRV SG +SEI L KYKFP
Sbjct: 361 ICGVGGTLTAIDNLGQIGNSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFP 420

Query: 421 RPLMLSLMLLFSCAGYLMVAFDVQNGLYAASIVIGFCFGAQWPLIYAIISELFGLKYYST 480
           RPLML+++LL SCAG+L++AF+V  GLY AS++IGFCFGAQWPL++AIISE+FGLKYYST
Sbjct: 421 RPLMLTMVLLLSCAGHLLIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYST 480

Query: 481 LFNFGSVASPIGLYVLNVKVKGNFYDREAEKQLRAKGLVRKAGEELKCFGAECFKLSFIV 540
           L+NFGSVASPIG Y+LNV+V G  YD EA KQ +A G  R  G++L C G  CFKLSFI+
Sbjct: 481 LYNFGSVASPIGSYLLNVRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFII 540

Query: 541 ITGVTLLGMLVSLILVIRTRSFYKSDIYKKFREE---AETEVAGNGAVVAAGAPEE 589
           I  VTL G+LVS++LVIRT+ FYKSDIYKKFRE+   AE E+A   A  +  A E+
Sbjct: 541 IAAVTLFGVLVSMVLVIRTKKFYKSDIYKKFREKALAAEMEMAAPAAARSTVAKED 588

[Close]

BLAST of Lag0040471 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 671.4 bits (1731), Expect = 6.7e-193
Identity = 333/551 (60.44%), Postives = 421/551 (76.41%), Query Frame = 0


Query: 23  VHVITGRWFVVFASLLIMAANGGIFMFGLYSNDIKSVLGYDQTTLNLLSFFKDLGANVGV 82
           +H   GRWF+VFAS LIMA  G  ++FG YS DIKS LGYDQTTLNLL FFKDLGANVGV
Sbjct: 11  IHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGANVGV 70

Query: 83  LSGLINEVSPPWVVLSSGAALNFFGYFMIWLAVTKRISTPKVWQMCVYICIGGNSQSFAN 142
           LSGLI EV+P W VL+ G+A+NF GYFMIWL VT +++ PKVWQMC+YICIG NSQ+FAN
Sbjct: 71  LSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQNFAN 130

Query: 143 TGSLVTCVKNFPESRGIVIGILKGYFGLSGAITTQLFHAFYGSDTKSLILLLGWLPAAIS 202
           TG+LVTCVKNFPESRG+++G+LKGY GLSGAI TQL+ A YG D+KSLILL+ WLPAA+S
Sbjct: 131 TGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLILLIAWLPAAVS 190

Query: 203 FAFLRTIRIMKVIRHPNELKVFYNFLYISLALAGFLMLMIIIESKLVLTQSAYGGSATVV 262
             F+  IR  KV+R  NEL VFY FLYIS+ LA FLM M I E ++  +++AY  SAT+ 
Sbjct: 191 LVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAASATIC 250

Query: 263 LLLLFLPLAVVVIEEYNLWKLKTAAIGAPNPIQIITEKPNIPKTEHQKQTQQNQKQKQPS 322
             LLF+PL V V +E  +W +    I  P+ +++   K  +   + +      ++++  S
Sbjct: 251 CALLFVPLTVSVKQELEVWNMMKLPIEEPSEVKVEKPKKELDLDQDKAAKVNGEEKETKS 310

Query: 323 CWTTIFSPPERGEDFTILQALFSMDMLVLFFSATCGVGGTLTVIDNLGQIGMSLGYPKRS 382
           C++T+FSPP RGED+TILQAL S DM++LF +  CG+G +LT +DNLGQIG SLGYP  +
Sbjct: 311 CFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGESLGYPNHT 370

Query: 383 IGTFVTLVSIWNYLGRVASGFISEIVLTKYKFPRPLMLSLMLLFSCAGYLMVAFDVQNGL 442
           + +FV+LVSIWNY GRV SGF+SE +L KYK PRPLM++L+LL SCAG+L++AF V   +
Sbjct: 371 VSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLIAFPVPGSV 430

Query: 443 YAASIVIGFCFGAQWPLIYAIISELFGLKYYSTLFNFGSVASPIGLYVLNVKVKGNFYDR 502
           Y ASI++GF FGAQ PL++AIISELFGLKYYSTLFN G +ASP+G Y+LNV+V G  YD+
Sbjct: 431 YIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNVRVTGMLYDK 490

Query: 503 EAEKQLRAKGLVRKAGEELKCFGAECFKLSFIVITGVTLLGMLVSLILVIRTRSFYKSDI 562
           EA KQL A+GL RK  ++L C G++C+KL F+++  VT  G LVSL L IRTR FYK DI
Sbjct: 491 EALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIRTREFYKGDI 550

Query: 563 YKKFREEAETE 574
           YKKFRE  E+E
Sbjct: 551 YKKFRESPESE 561

[Close]

BLAST of Lag0040471 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 331.3 bits (848), Expect = 1.6e-90
Identity = 211/583 (36.19%), Postives = 326/583 (55.92%), Query Frame = 0


Query: 16  SDIRTIAVHVITGRWFVVFASLLIMAANGGIFMFGLYSNDIKSVLGYDQTTLNLLSFFKD 75
           S  R   V  I  RW V  A++ I +  G  ++FG  S  IKS L Y+Q  L+ L   KD
Sbjct: 3   STTREKFVSFINNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKD 62

Query: 76  LGANVGVLSGLINEVSPPWVVLSSGAALNFFGYFMIWLAVTKRISTPKVWQMCVYICIGG 135
           LG +VG ++G ++E+ P W  L  GA  N  GY  +WL VT R     +W MCV I +G 
Sbjct: 63  LGDSVGFIAGTLSEILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGN 122

Query: 136 NSQSFANTGSLVTCVKNFPESRGIVIGILKGYFGLSGAITTQLFHAFYGSDTKSLILLLG 195
           N +++ NTG+LV+ V+NFP+SRG V+GILKG+ GL GAI +Q++   + S+  SLIL++ 
Sbjct: 123 NGETYFNTGALVSGVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSNPASLILMVA 182

Query: 196 WLPAAISFA---FLRTIRIMKVIRHPNELKVFYNFLY-ISLALAGFLMLMIIIESKLVLT 255
             PA +      F+R +   K IR  +     + F+Y + L LA +LM +++I+  +V++
Sbjct: 183 VTPAVVVVCLMFFIRPVGGHKQIRPTDGAS--FTFIYGVCLLLAAYLMSVMLIQDLVVVS 242

Query: 256 QSAYGGSATVVLLLLFLPLAVVV--------------IEEYNLWKLKTAAIGAPNPIQII 315
            +       V+ ++L +P+ V +              IEE  + K +    G   P  I+
Sbjct: 243 HNVITVFTIVLFVILVVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPDLIL 302

Query: 316 T----EKPN----IPKTEHQK-----QTQQNQKQKQPSCWTTIFSPPERGEDFTILQALF 375
           +    EKP     +P +E  K     Q Q  Q   + +        P RGEDFT+ QAL 
Sbjct: 303 SEVEDEKPKDVDLLPASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALV 362

Query: 376 SMDMLVLFFSATCGVGGTLTVIDNLGQIGMSLGYPKRSIGTFVTLVSIWNYLGRVASGFI 435
             D  ++FFS   G G  LTVIDNLGQ+  SLGY    +   V+++SIWN+LGR+  G+ 
Sbjct: 363 KADFWLIFFSLLLGSGSGLTVIDNLGQMSQSLGYDNTHV--LVSMISIWNFLGRIGGGYF 422

Query: 436 SEIVLTKYKFPRPLMLSLMLLFSCAGYLMVAFDVQNGLYAASIVIGFCFGAQWPLIYAII 495
           SE+V+  Y +PRP+ +++  L    G++  A+     +Y  +++IG  +GA W ++ A  
Sbjct: 423 SELVVRDYAYPRPVAMAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATA 482

Query: 496 SELFGLKYYSTLFNFGSVASPIGLYVLNVKVKGNFYDREAEKQLRAKGLVRKAGEELKCF 555
           SELFGLK +  L+NF ++A+P G  V +  +  + YDREAE+Q  A G V    + L+C 
Sbjct: 483 SELFGLKKFGALYNFLTLANPAGSLVFSGMIASSIYDREAERQ--AHGSVFDPDDALRCN 542

Query: 556 GAECFKLSFIVITGVTLLGMLVSLILVIRTRSFYKSDIYKKFR 568
           G+ CF L+ ++++G  ++  ++S+ILV RT+S Y + +Y K R
Sbjct: 543 GSICFFLTSLIMSGFCIIACMLSMILVRRTKSVY-THLYGKTR 578

[Close]

BLAST of Lag0040471 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 319.3 bits (817), Expect = 6.4e-87
Identity = 193/559 (34.53%), Postives = 307/559 (54.92%), Query Frame = 0


Query: 23  VHVITGRWFVVFASLLIMAANGGIFMFGLYSNDIKSVLGYDQTTLNLLSFFKDLGANVGV 82
           + ++  +W  + AS+ I  A+G  + FG+YS  +KS   YDQ+TL+ +S FKD+GAN GV
Sbjct: 1   MEILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGV 60

Query: 83  LSGL----------------INEVSPPWVVLSSGAALNFFGYFMIWLAVTKRISTPKVWQ 142
            SGL                I     PWVVL+ GA   F GYF+IW +VT  I  P V  
Sbjct: 61  FSGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPL 120

Query: 143 MCVYICIGGNSQSFANTGSLVTCVKNFPESRGIVIGILKGYFGLSGAITTQLFHAFYGSD 202
           MC+++ +   SQ+F NT ++V+ V+NF +  G  +GI+KG+ GLSGAI  QL+      D
Sbjct: 121 MCLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGD 180

Query: 203 TKSLILLLGWLPAAISFAFLRTIRIMKVIRHPNELKVFYNFLYISLALAGFLMLMIIIES 262
             S ILLL   P  +S   +  +RI +     ++ K       +SL +A +LM++II+++
Sbjct: 181 PASFILLLAVTPTVLSLLVMPLVRIYET-SVADDKKHLNGLSAVSLIIAAYLMIIIILKN 240

Query: 263 KLVLTQSAYGGSATVVLLLLFLPLAVVVIEEYNLWKLKTAAIGAPNPIQIITEKPNIPKT 322
              L  S++    T+V LL+ L L +++        ++      P+    +   P    +
Sbjct: 241 TFGL--SSWANIVTLVCLLVMLALPLLIARRAQRDGMEKT---VPHDYSPLISSPKATTS 300

Query: 323 EHQKQTQQNQKQKQPSCWTTIFSPPERGEDFTILQALFSMDMLVLFFSATCGVGGTLTVI 382
            +Q     ++ +   S            E+  +LQA+  +   +LF +  CG+G  L+ I
Sbjct: 301 GNQSSEGDSKVEAGLS------------ENLNLLQAMKKLSFWLLFLAMICGMGSGLSTI 360

Query: 383 DNLGQIGMSLGYPKRSIGTFVTLVSIWNYLGRVASGFISEIVLTKYKFPRPLMLSLMLLF 442
           +N+ QIG SL Y    I + V+L SIWN+LGR  +G+ S+ +L K  +PRPL+++  L  
Sbjct: 361 NNIRQIGESLRYSSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATLGT 420

Query: 443 SCAGYLMVAFDVQNGLYAASIVIGFCFGAQWPLIYAIISELFGLKYYSTLFNFGSVASPI 502
              G+L++A   Q  LY  S+++G C+G+QW L+  I SELFG+++  T+FN  SVASPI
Sbjct: 421 MSIGHLIIASGFQGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVASPI 480

Query: 503 GLYVLNVKVKGNFYDREAEKQLRAKGLVRKAGEELKCFGAECFKLSFIVITGVTLLGMLV 562
           G Y+ +V++ G  YD+ A            +GE   C+G+ CF+LSFI++  V   G LV
Sbjct: 481 GSYIFSVRLIGYIYDKTA------------SGEGNTCYGSHCFRLSFIIMASVAFFGFLV 529

Query: 563 SLILVIRTRSFYKSDIYKK 566
           +++L  RT++ Y+  + K+
Sbjct: 541 AIVLFFRTKTLYRQILVKR 529

[Close]

BLAST of Lag0040471 vs. TAIR 10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 318.5 bits (815), Expect = 1.1e-86
Identity = 193/559 (34.53%), Postives = 308/559 (55.10%), Query Frame = 0


Query: 21  IAVHVITGRWFVVFASLLIMAANGGIFMFGLYSNDIKSVLGYDQTTLNLLSFFKDLGANV 80
           +A  ++  +W  + AS+ I  + GG + FG+YS  +KS   YDQ+TL+ +S FKD+G NV
Sbjct: 1   MATEILRTKWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNV 60

Query: 81  GVLSGLINEVSP--------------PWVVLSSGAALNFFGYFMIWLAVTKRISTPKVWQ 140
           GVLSGL+   +               PWVV+  GA LNF GYF++W +VT  I  P V  
Sbjct: 61  GVLSGLVYTAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPV 120

Query: 141 MCVYICIGGNSQSFANTGSLVTCVKNFPESRGIVIGILKGYFGLSGAITTQLFHAFYGSD 200
           MC+++ I   S +F NT ++V+ ++NF +  G  +GI+KG+ GLSGA+  QL+      D
Sbjct: 121 MCLFMFIAAQSLTFLNTANVVSSLENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGD 180

Query: 201 TKSLILLLGWLPAAISFAFLRTIRIMKVIRHPNELKVFYNFLYISLALAGFLMLMIIIES 260
            K+ ILLL  +P+ +S   +  +R+ K     +E K       +SL +A +LM+ II++S
Sbjct: 181 PKTFILLLAIVPSLLSVLVMPLVRVYKT-STVDEKKHLDGLSTLSLIIAAYLMITIILKS 240

Query: 261 KLVLTQSAYGGSATVVLLLLFLPLAVVVIEEYNLWKLKTAAIGAPNPIQIITEKPNIPKT 320
            L L   A   +  V+L+LL  PL V V         +        P+  +      P  
Sbjct: 241 TLSLPSWANAVTLAVLLVLLSSPLLVAV---------RAHRDSIEKPLSSVYS----PLV 300

Query: 321 EHQKQTQQNQKQKQPSCWTTIFSPPERGEDFTILQALFSMDMLVLFFSATCGVGGTLTVI 380
           ++ + T   +               +  +   +LQA+ ++D  +LF +  CG+G  ++ I
Sbjct: 301 DNLEATTSGE-----------ILMLDEDKSLNLLQAMCNVDFWLLFLAMICGMGSGISTI 360

Query: 381 DNLGQIGMSLGYPKRSIGTFVTLVSIWNYLGRVASGFISEIVLTKYKFPRPLMLSLMLLF 440
           +N+ QIG SL Y    I + + L +IWN++GR   G++S+ +L +  +PRPL+++  L  
Sbjct: 361 NNIRQIGESLRYTSVEINSLLALWNIWNFIGRFGGGYVSDWLLHRKGWPRPLLMATTLGT 420

Query: 441 SCAGYLMVAFDVQNGLYAASIVIGFCFGAQWPLIYAIISELFGLKYYSTLFNFGSVASPI 500
              G+L++A   Q  LY  SI++G C+G+QW L+  I SELFG+K+  T++N  S+ASP+
Sbjct: 421 MTIGHLIIASGFQGNLYPGSIIVGICYGSQWSLMPTITSELFGVKHMGTIYNTISIASPM 480

Query: 501 GLYVLNVKVKGNFYDREAEKQLRAKGLVRKAGEELKCFGAECFKLSFIVITGVTLLGMLV 560
           G Y+ +V++ G  YDR               GE   C+G  CF+L+++VI  V  LG LV
Sbjct: 481 GSYIFSVRLIGYIYDRTI------------IGEGNTCYGPHCFRLAYVVIASVAFLGFLV 521

Query: 561 SLILVIRTRSFYKSDIYKK 566
           S +LV RT++ Y+  I++K
Sbjct: 541 SCVLVFRTKTIYR-QIFEK 521

The following BLAST results are available for this feature:

BLAST of Lag0040471 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6573851.1	4.1e-285	85.47	Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_022944943.1	4.5e-284	85.76	protein NUCLEAR FUSION DEFECTIVE 4 [Cucurbita moschata]	[more]
XP_023541417.1	2.1e-281	84.92	protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo]	[more]
XP_022956604.1	2.7e-281	85.20	protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata]	[more]
KAG6601185.1	2.7e-281	85.03	Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror...	[more]

BLAST of Lag0040471 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4I9E1	1.6e-29	24.86	Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ...	[more]
Q59MJ2	1.2e-05	27.53	Probable transporter MCH1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX...	[more]

BLAST of Lag0040471 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FZF2	2.2e-284	85.76	protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucurbita moschata OX=3662 GN=LOC111449326...	[more]
A0A6J1GZI8	1.3e-281	85.20	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1K186	5.2e-278	84.89	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111489...	[more]
A0A5D3CFW3	2.0e-277	84.79	Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3BG09	2.0e-277	84.79	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103489435 ...	[more]

BLAST of Lag0040471 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G39210.1	3.8e-228	66.28	Major facilitator superfamily protein	[more]
AT2G28120.1	6.7e-193	60.44	Major facilitator superfamily protein	[more]
AT5G14120.1	1.6e-90	36.19	Major facilitator superfamily protein	[more]
AT1G74780.1	6.4e-87	34.53	Nodulin-like / Major Facilitator Superfamily protein	[more]
AT1G18940.1	1.1e-86	34.53	Nodulin-like / Major Facilitator Superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036259	MFS transporter superfamily	GENE3D	1.20.1250.20	MFS general substrate transporter like domains	coord: 259..568 e-value: 7.4E-9 score: 36.9
IPR036259	MFS transporter superfamily	SUPERFAMILY	103473	MFS general substrate transporter	coord: 28..556
IPR010658	Nodulin-like	PFAM	PF06813	Nodulin-like	coord: 29..274 e-value: 2.1E-91 score: 305.9
None	No IPR available	PANTHER	PTHR21576	UNCHARACTERIZED NODULIN-LIKE PROTEIN	coord: 11..575
None	No IPR available	PANTHER	PTHR21576:SF122	MFS TRANSPORTER	coord: 11..575
None	No IPR available	CDD	cd17354	MFS_Mch1p_like	coord: 28..544 e-value: 2.1809E-107 score: 326.131

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0040471.1	Lag0040471.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0099587	inorganic ion import across plasma membrane
biological_process	GO:0002239	response to oomycetes
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0022857	transmembrane transporter activity