Lag0040467 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0040467 vs. NCBI nr
Match: XP_038892369.1 (pullulanase 1, chloroplastic [Benincasa hispida] >XP_038892370.1 pullulanase 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 1793.9 bits (4645), Expect = 0.0e+00
Identity = 869/967 (89.87%), Postives = 915/967 (94.62%), Query Frame = 0

Query: 146  MSLLFSSSLHLLPSSTPVTSFRYILPTRIAAFSSSPPPGGLFSAVAGTSPSRYPDDHFRG 205
            MSLLFSSSLHLLPSSTP TS  Y    RIAAFSSSPP GG FS V+ TS S + DD+FR 
Sbjct: 1    MSLLFSSSLHLLPSSTPATSICYQAHPRIAAFSSSPPAGGFFS-VSATSLSHFLDDNFRS 60

Query: 206  GPLYCSSSASMSLEESAASTSQLEDSLLYSRAFWVSRYVIAWNVEVGNGSCYLFASKMAT 265
            G LYCSSS+SMSLEES  STSQLEDSLLYSRAFWVSRYVIAWNVEVG+GSCYLFASKMAT
Sbjct: 61   GSLYCSSSSSMSLEES-TSTSQLEDSLLYSRAFWVSRYVIAWNVEVGSGSCYLFASKMAT 120

Query: 266  LRVKGGVVEGYDAKIKLEKDYGPLTENVIKKFPHIQNYCPFTVPPESDVETLLKYQLAVA 325
            LR++ GVVEGYD K+KLEKD G L ENVIKKFPHIQNYCPFTVP  SDVE LLK QLAVA
Sbjct: 121  LRIEDGVVEGYDVKLKLEKDRGRLPENVIKKFPHIQNYCPFTVPLASDVEALLKCQLAVA 180

Query: 326  TFNSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYRDPV 385
            TFNSYG+CNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAV+AQI+RDPV
Sbjct: 181  TFNSYGECNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVRAQIFRDPV 240

Query: 386  GGMPFEVIQLEELDGVWRTKGPRSWEGCYYEYEVTVYHPSTLQVEKCFTTDPYARGVSSD 445
            GG PFEVIQLEELDGVWRTKGP+SWEGCYYEYEVTVYHPSTLQ+EKCFTTDPY+RG+SSD
Sbjct: 241  GGTPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLQIEKCFTTDPYSRGISSD 300

Query: 446  GRRTLFVDLGSDDLKPEGWDKLADKKPPVNSFSDISIYELHIRDFSISDQSVHPDLRGGY 505
            GRRTLFVD+GS+DL P+GWDKLAD+K PV+SFSDISIYELH+RDFSISDQSVHPDLRGG+
Sbjct: 301  GRRTLFVDIGSNDLIPKGWDKLADEKHPVDSFSDISIYELHVRDFSISDQSVHPDLRGGF 360

Query: 506  MAFTLQDSAGITHLKKLSNAGLSHVHLLPTFQFAGVDDDKKKWKSLDTKPLENLPPDSAE 565
            MAFTLQDSAGI HLKKLSNAG+SHVHLLPTFQF GVDDDK KWK  DT+ LE+LPPDSAE
Sbjct: 361  MAFTLQDSAGINHLKKLSNAGISHVHLLPTFQFGGVDDDKTKWKFFDTELLEDLPPDSAE 420

Query: 566  QQALIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLHVVLD 625
            Q++LIADI NSDGYNWGYNPILWGVPKGSYASDPNGPCR+IEFRKMVQALNQIGL VVLD
Sbjct: 421  QESLIADILNSDGYNWGYNPILWGVPKGSYASDPNGPCRVIEFRKMVQALNQIGLRVVLD 480

Query: 626  VVYNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDIL 685
            VVYNHLHGHGPFDPNSVLDKIVPGYYLRRN DGFIENSTCVNNTASEHFMVERMIVDDIL
Sbjct: 481  VVYNHLHGHGPFDPNSVLDKIVPGYYLRRNADGFIENSTCVNNTASEHFMVERMIVDDIL 540

Query: 686  HWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQSLTKEKNGVDGSSIYLYGEGWDFGEVAR 745
            HWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQ LTKEKNGVDGSSIY+YGEGWDFGEVA+
Sbjct: 541  HWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQGLTKEKNGVDGSSIYIYGEGWDFGEVAK 600

Query: 746  NGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDEV 805
            NGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTD+V
Sbjct: 601  NGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDKV 660

Query: 806  AEFMLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYASCPTETVNYAS 865
            AE MLAVSKDHI+VAMAANLRDYVLTNFEG+EVKGLEVLTHDGSPVAYASCP+ETVNY S
Sbjct: 661  AESMLAVSKDHIEVAMAANLRDYVLTNFEGQEVKGLEVLTHDGSPVAYASCPSETVNYVS 720

Query: 866  AHDNETLFDVVSLKTPINITLNERCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDS 925
            AHDNETLFD+VSLKTP NI +++RCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDS
Sbjct: 721  AHDNETLFDIVSLKTPRNIAVDDRCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDS 780

Query: 926  YNSGDWFNRLDFTYMTNNWGVGLPPKEKNENNWPLIRPRLADPSFKPSKSHILAAVENFT 985
            YNSGDWFNRLDFTY TNNWGVGLPPKEKNE NWPLI+PRLADPSFKPSK+HILAAVEN T
Sbjct: 781  YNSGDWFNRLDFTYTTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKTHILAAVENCT 840

Query: 986  NLLQIRYSSPLFRLKTSNAIQKRVRFHNTGASLIPGVIVMSIEDGHEGITGLSQLDPTYS 1045
            NLLQIRYSSPLFRLKTSNAIQ+RVRFHN+GASLIPG+IVMSIEDGHEGI GLSQLD  YS
Sbjct: 841  NLLQIRYSSPLFRLKTSNAIQRRVRFHNSGASLIPGLIVMSIEDGHEGIPGLSQLDSIYS 900

Query: 1046 YIVVVVNARPTEISFPCPALRARTLQLHPIQLMSTDPVVKNSIYEPSTGCFMVPSRTTSV 1105
            YIVVVVNARPTEISFPCPALRARTLQLHPIQLMSTDP+VKNS YEPSTGCFMVP RTT+V
Sbjct: 901  YIVVVVNARPTEISFPCPALRARTLQLHPIQLMSTDPIVKNSTYEPSTGCFMVPPRTTAV 960

Query: 1106 FVEPRIH 1113
            FVEPRI+
Sbjct: 961  FVEPRIY 965

BLAST of Lag0040467 vs. NCBI nr
Match: XP_022945721.1 (pullulanase 1, chloroplastic [Cucurbita moschata])

HSP 1 Score: 1783.1 bits (4617), Expect = 0.0e+00
Identity = 871/967 (90.07%), Postives = 906/967 (93.69%), Query Frame = 0

Query: 146  MSLLFSSSLHLLPSSTPVTSFRYILPTRIAAFSSSPPPGGLFSAVAGTSPSRYPDDHFRG 205
            MS LFSSSLHLLP S P TSF + LP RIAA SSSP  GG+FS VA TS S YP  H R 
Sbjct: 1    MSFLFSSSLHLLPPSNPATSFSHHLPPRIAAVSSSPAVGGVFS-VAATSLSHYPHGHSRC 60

Query: 206  GPLYCSSSASMSLEESAASTSQLEDSLLYSRAFWVSRYVIAWNVEVGNGSCYLFASKMAT 265
            GPLYCSSSASMSLEES  + SQLEDSLLY+RAFWVSRYVIAWNVEVG+GSCYLF SKMAT
Sbjct: 61   GPLYCSSSASMSLEESTPA-SQLEDSLLYARAFWVSRYVIAWNVEVGSGSCYLFGSKMAT 120

Query: 266  LRVKGGVVEGYDAKIKLEKDYGPLTENVIKKFPHIQNYCPFTVPPESDVETLLKYQLAVA 325
            LRV  GVVEGYD KIKLEKD GPL ENVIKKFPHIQNYCPFTVP  S VE LLK QLAVA
Sbjct: 121  LRVNDGVVEGYDVKIKLEKDRGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVA 180

Query: 326  TFNSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYRDPV 385
            TFNSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIY++PV
Sbjct: 181  TFNSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYKEPV 240

Query: 386  GGMPFEVIQLEELDGVWRTKGPRSWEGCYYEYEVTVYHPSTLQVEKCFTTDPYARGVSSD 445
            GG PFEVIQLEELDGVWRTKGP+SWEGCYYEYEVTVYHPSTL+VEKCF+TDPY+RGVSSD
Sbjct: 241  GGRPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGVSSD 300

Query: 446  GRRTLFVDLGSDDLKPEGWDKLADKKPPVNSFSDISIYELHIRDFSISDQSVHPDLRGGY 505
            GRRTLFVDLGSDDLKPEGWDKLAD+KPP++SFSDISIYELH+RDFSISDQSVHPD+RGGY
Sbjct: 301  GRRTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPDMRGGY 360

Query: 506  MAFTLQDSAGITHLKKLSNAGLSHVHLLPTFQFAGVDDDKKKWKSLDTKPLENLPPDSAE 565
            M FTLQDS GITHLKKLSNAG+SHVHLLPTFQF GVDDDK KWKSLD + LE LPPDSAE
Sbjct: 361  MGFTLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAE 420

Query: 566  QQALIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLHVVLD 625
            QQ+LIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGL VVLD
Sbjct: 421  QQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLD 480

Query: 626  VVYNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDIL 685
            VVYNHLHG GPFDP+SVLDKIVPGYYLRRN DGFIENSTCVNNTASEHFMVERMIVDDIL
Sbjct: 481  VVYNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDIL 540

Query: 686  HWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQSLTKEKNGVDGSSIYLYGEGWDFGEVAR 745
            HWVVDYKVDGFRFDLMGHLM STMLKAK+ALQ LT+EKNGVDGSSIYLYGEGWDFGEVA+
Sbjct: 541  HWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWDFGEVAK 600

Query: 746  NGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDEV 805
            NGRGVNASQFNLFGTGIGSFNDRVRDA+LGGSPFGHPLQQGFVTGLLLEPNDHDHGTD+ 
Sbjct: 601  NGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGTDKD 660

Query: 806  AEFMLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYASCPTETVNYAS 865
            AEF LAVSKDHIQVAMAANL+DYVL NFEGKEVKG EV T+DGS VAYASCPTETVNY S
Sbjct: 661  AEFTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSVAYASCPTETVNYVS 720

Query: 866  AHDNETLFDVVSLKTPINITLNERCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDS 925
            AHDNETLFDVVSLKTP NIT+N+RCR+NHLATSIIALSQGIPFFHCGDELLRSKSMDRDS
Sbjct: 721  AHDNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDELLRSKSMDRDS 780

Query: 926  YNSGDWFNRLDFTYMTNNWGVGLPPKEKNENNWPLIRPRLADPSFKPSKSHILAAVENFT 985
            YNSGDWFNRLDFTYMTNNWGVGLPPKEKNE NWPLI+PRLADPSFKPSKSHILAAVENFT
Sbjct: 781  YNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFT 840

Query: 986  NLLQIRYSSPLFRLKTSNAIQKRVRFHNTGASLIPGVIVMSIEDGHEGITGLSQLDPTYS 1045
            NLLQIRYSSPLFRLKT+NAIQKRVRFHNTG SLIPGVIVMSIEDG EGI GL+QLDP YS
Sbjct: 841  NLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYS 900

Query: 1046 YIVVVVNARPTEISFPCPALRARTLQLHPIQLMSTDPVVKNSIYEPSTGCFMVPSRTTSV 1105
            YIVVVVNARP+EISFPCPALRARTLQLHPIQLMSTDP+VKNS YEPSTGCFMVPSRTTSV
Sbjct: 901  YIVVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSV 960

Query: 1106 FVEPRIH 1113
            FVE R H
Sbjct: 961  FVELRKH 965

BLAST of Lag0040467 vs. NCBI nr
Match: KAG6573853.1 (Pullulanase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1779.6 bits (4608), Expect = 0.0e+00
Identity = 870/967 (89.97%), Postives = 904/967 (93.49%), Query Frame = 0

Query: 146  MSLLFSSSLHLLPSSTPVTSFRYILPTRIAAFSSSPPPGGLFSAVAGTSPSRYPDDHFRG 205
            MS LFSSSLHLLP S P TSF + LP RIAA SSSP  GG+FS VA TS S YPD H R 
Sbjct: 1    MSFLFSSSLHLLPPSNPATSFSHHLPPRIAAVSSSPAVGGVFS-VAATSLSHYPDGHSRC 60

Query: 206  GPLYCSSSASMSLEESAASTSQLEDSLLYSRAFWVSRYVIAWNVEVGNGSCYLFASKMAT 265
            GPLYCSSSASMSLEES  + SQLEDSLLY+RAFWVSRYVIAWNVEV +GSCYLF SKMAT
Sbjct: 61   GPLYCSSSASMSLEESTPA-SQLEDSLLYARAFWVSRYVIAWNVEVRSGSCYLFGSKMAT 120

Query: 266  LRVKGGVVEGYDAKIKLEKDYGPLTENVIKKFPHIQNYCPFTVPPESDVETLLKYQLAVA 325
            LRV  GVVEGYD KIKLEKD GPL ENVIKKFPHIQNYCPFTVP  S VE LLK QLAVA
Sbjct: 121  LRVNDGVVEGYDVKIKLEKDSGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVA 180

Query: 326  TFNSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYRDPV 385
            TFNSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKE VSLYLWAPTAQAVQAQIY++PV
Sbjct: 181  TFNSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEVVSLYLWAPTAQAVQAQIYKEPV 240

Query: 386  GGMPFEVIQLEELDGVWRTKGPRSWEGCYYEYEVTVYHPSTLQVEKCFTTDPYARGVSSD 445
            GG PFEVIQLEELDGVWRTKGP+SWEGCYYEYEVTVYHPSTL+VEKCF+TDPY+RGVSSD
Sbjct: 241  GGRPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGVSSD 300

Query: 446  GRRTLFVDLGSDDLKPEGWDKLADKKPPVNSFSDISIYELHIRDFSISDQSVHPDLRGGY 505
            GRRTLFVDLGSDDLKPEGWDKLAD+KPP++SFSDISIYELH+RDFSISDQSVHPD+RGGY
Sbjct: 301  GRRTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPDMRGGY 360

Query: 506  MAFTLQDSAGITHLKKLSNAGLSHVHLLPTFQFAGVDDDKKKWKSLDTKPLENLPPDSAE 565
            M FTLQDS GITHLKKLSNAG+SHVHLLPTFQF GVDDDK KWKSLD + LE LPPDSAE
Sbjct: 361  MGFTLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAE 420

Query: 566  QQALIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLHVVLD 625
            QQ+LIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGL VVLD
Sbjct: 421  QQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLD 480

Query: 626  VVYNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDIL 685
            VVYNHLHG GPFDP SVLDKIVPGYYLRRN DGFIENSTCVNNTASEHFMVERMIVDDIL
Sbjct: 481  VVYNHLHGCGPFDPISVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDIL 540

Query: 686  HWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQSLTKEKNGVDGSSIYLYGEGWDFGEVAR 745
            HWVVDYKVDGFRFDLMGHLM STMLKAK+ALQ LT+EKNGVDGSSIYLYGEGWDFGEVA+
Sbjct: 541  HWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWDFGEVAK 600

Query: 746  NGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDEV 805
            NGRGVNASQFNLFGTGIGSFNDRVRDA+LGGSPFGHPLQQGFVTGLLLEPNDHDHGTD+ 
Sbjct: 601  NGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGTDKD 660

Query: 806  AEFMLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYASCPTETVNYAS 865
            AEF LAVSKDHIQVAMAANL+DYVL NFEGKEVKG EV T+DGS VAYASCPTETVNY S
Sbjct: 661  AEFTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSVAYASCPTETVNYVS 720

Query: 866  AHDNETLFDVVSLKTPINITLNERCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDS 925
            AHDNETLFDVVSLKTP NIT+N+RCR+NHLATSIIALSQGIPFFHCGDELLRSKSMDRDS
Sbjct: 721  AHDNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDELLRSKSMDRDS 780

Query: 926  YNSGDWFNRLDFTYMTNNWGVGLPPKEKNENNWPLIRPRLADPSFKPSKSHILAAVENFT 985
            YNSGDWFNRLDFTYMTNNWGVGLPPKEKNE NWPLI+PRLADPSFKPSKSHILAAVENFT
Sbjct: 781  YNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFT 840

Query: 986  NLLQIRYSSPLFRLKTSNAIQKRVRFHNTGASLIPGVIVMSIEDGHEGITGLSQLDPTYS 1045
            NLLQIRYSSPLFRLKT+NAIQKRVRFHNTG SLIPGVIVMSIEDG EGI GL+QLDP YS
Sbjct: 841  NLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYS 900

Query: 1046 YIVVVVNARPTEISFPCPALRARTLQLHPIQLMSTDPVVKNSIYEPSTGCFMVPSRTTSV 1105
            YIVVVVNARP+EISFPCPALRARTLQLHPIQLMSTDP+VKNS YEPSTGCFMVPSRTTSV
Sbjct: 901  YIVVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSV 960

Query: 1106 FVEPRIH 1113
            FVE R H
Sbjct: 961  FVELRKH 965

BLAST of Lag0040467 vs. NCBI nr
Match: XP_004142505.1 (pullulanase 1, chloroplastic isoform X1 [Cucumis sativus])

HSP 1 Score: 1764.6 bits (4569), Expect = 0.0e+00
Identity = 861/967 (89.04%), Postives = 901/967 (93.17%), Query Frame = 0

Query: 146  MSLLFSSSLHLLPSSTPVTSFRYILPTRIAAFSSSPPPGGLFSAVAGTSPSRYPDDHFRG 205
            MSLLFSSSLH  P S   TS  Y L    AAFSSSPP        AGTS S     +FR 
Sbjct: 1    MSLLFSSSLHFFPPSAFPTSLCYQLHHPFAAFSSSPP-------AAGTSLSPGFRHNFRT 60

Query: 206  GPLYCSSSASMSLEESAASTSQLEDSLLYSRAFWVSRYVIAWNVEVGNGSCYLFASKMAT 265
            GPLYCS S+SMSLEES  STSQLEDSLLYSRAFWVS++VIAWNVEV +GSCYLFASKMAT
Sbjct: 61   GPLYCSPSSSMSLEES-TSTSQLEDSLLYSRAFWVSKHVIAWNVEVESGSCYLFASKMAT 120

Query: 266  LRVKGGVVEGYDAKIKLEKDYGPLTENVIKKFPHIQNYCPFTVPPESDVETLLKYQLAVA 325
            LRV+ GVVEGYD KI+LEKD   L ENVIKKFPHIQNYCPFTVPP SDVE LLK QLAVA
Sbjct: 121  LRVEDGVVEGYDVKIRLEKDRSQLPENVIKKFPHIQNYCPFTVPPASDVEALLKCQLAVA 180

Query: 326  TFNSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYRDPV 385
            TFNSYG+C NITCLQLPGVLDDLFSYEGPLGA+YSKEAVSLYLWAPTAQAV+AQI+RDPV
Sbjct: 181  TFNSYGECKNITCLQLPGVLDDLFSYEGPLGAIYSKEAVSLYLWAPTAQAVRAQIFRDPV 240

Query: 386  GGMPFEVIQLEELDGVWRTKGPRSWEGCYYEYEVTVYHPSTLQVEKCFTTDPYARGVSSD 445
            GGMPFEVI LEE+DG+WRTKGP+SW+GCYYEYEVTVYHPSTLQVEKCFTTDPY+RGVSSD
Sbjct: 241  GGMPFEVIPLEEVDGIWRTKGPKSWKGCYYEYEVTVYHPSTLQVEKCFTTDPYSRGVSSD 300

Query: 446  GRRTLFVDLGSDDLKPEGWDKLADKKPPVNSFSDISIYELHIRDFSISDQSVHPDLRGGY 505
            GRRTLFVDL SDDL P+GWDKLAD+KPPV+SFSDISIYELH+RDFSISDQSVHPDLRGGY
Sbjct: 301  GRRTLFVDLFSDDLIPKGWDKLADEKPPVDSFSDISIYELHVRDFSISDQSVHPDLRGGY 360

Query: 506  MAFTLQDSAGITHLKKLSNAGLSHVHLLPTFQFAGVDDDKKKWKSLDTKPLENLPPDSAE 565
            MAFTLQDSAGI HLKKLSNAGLSHVHLLPTFQF GVDDDK KWK +DT+ LENLPPDSAE
Sbjct: 361  MAFTLQDSAGINHLKKLSNAGLSHVHLLPTFQFGGVDDDKTKWKFVDTELLENLPPDSAE 420

Query: 566  QQALIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLHVVLD 625
            QQ+LIADIQNSDGYNWGYNPI+WGVPKGSYASD NGPCRL+EFRKMVQALNQIGL VVLD
Sbjct: 421  QQSLIADIQNSDGYNWGYNPIMWGVPKGSYASDSNGPCRLVEFRKMVQALNQIGLRVVLD 480

Query: 626  VVYNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDIL 685
            VVYNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDIL
Sbjct: 481  VVYNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDIL 540

Query: 686  HWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQSLTKEKNGVDGSSIYLYGEGWDFGEVAR 745
            HWVVDYKVDGFRFDLMGHLMKSTMLKAKDAL+ LTKEKNGVDGSSIY+YGEGWDFGEVA+
Sbjct: 541  HWVVDYKVDGFRFDLMGHLMKSTMLKAKDALRGLTKEKNGVDGSSIYIYGEGWDFGEVAK 600

Query: 746  NGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDEV 805
            NGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGT EV
Sbjct: 601  NGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTHEV 660

Query: 806  AEFMLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYASCPTETVNYAS 865
            AE MLAVSKDHI+VAMAANLRDY+LTNFEGKEVKGLEVLTHD SPVAYASCPTETVNY S
Sbjct: 661  AESMLAVSKDHIEVAMAANLRDYILTNFEGKEVKGLEVLTHDRSPVAYASCPTETVNYVS 720

Query: 866  AHDNETLFDVVSLKTPINITLNERCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDS 925
            AHDNETLFD+VSLKTP NIT+++RCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDS
Sbjct: 721  AHDNETLFDIVSLKTPRNITVDDRCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDS 780

Query: 926  YNSGDWFNRLDFTYMTNNWGVGLPPKEKNENNWPLIRPRLADPSFKPSKSHILAAVENFT 985
            YNSGDWFNRLDFTYMTNNWGVGLP KEKN+ NWPLI+PRLADPSFKPSKSHILAAVENFT
Sbjct: 781  YNSGDWFNRLDFTYMTNNWGVGLPLKEKNQYNWPLIKPRLADPSFKPSKSHILAAVENFT 840

Query: 986  NLLQIRYSSPLFRLKTSNAIQKRVRFHNTGASLIPGVIVMSIEDGHEGITGLSQLDPTYS 1045
            NLLQIRYSSPLFRLKTSNAIQKRVRFHN+G SLIPG+IVMSIEDGH GI GLSQLD TYS
Sbjct: 841  NLLQIRYSSPLFRLKTSNAIQKRVRFHNSGTSLIPGLIVMSIEDGHNGIPGLSQLDSTYS 900

Query: 1046 YIVVVVNARPTEISFPCPALRARTLQLHPIQLMSTDPVVKNSIYEPSTGCFMVPSRTTSV 1105
            YIVVVVNARPTEISFPCPALRA+TLQLHPIQLMSTDPVVKNS YEPSTGCFMVP RTTSV
Sbjct: 901  YIVVVVNARPTEISFPCPALRAKTLQLHPIQLMSTDPVVKNSTYEPSTGCFMVPPRTTSV 959

Query: 1106 FVEPRIH 1113
            FVEPR+H
Sbjct: 961  FVEPRMH 959

BLAST of Lag0040467 vs. NCBI nr
Match: XP_008446816.1 (PREDICTED: pullulanase 1, chloroplastic [Cucumis melo] >KAA0034691.1 pullulanase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1759.6 bits (4556), Expect = 0.0e+00
Identity = 861/967 (89.04%), Postives = 896/967 (92.66%), Query Frame = 0

Query: 146  MSLLFSSSLHLLPSSTPVTSFRYILPTRIAAFSSSPPPGGLFSAVAGTSPSRYPDDHFRG 205
            MSLLFSSSLH  PS    TS RY L  R AA SSSPP  GLFSA AGTS S     +FR 
Sbjct: 1    MSLLFSSSLHFFPSLASPTSLRYQLHPRFAASSSSPPAAGLFSA-AGTSLSHGSHHNFRT 60

Query: 206  GPLYCSSSASMSLEESAASTSQLEDSLLYSRAFWVSRYVIAWNVEVGNGSCYLFASKMAT 265
            GPLYCS S+SMSLEES  STSQLEDSLLYSRAFWVSR+VIAWNVEV +GSCYLFASKMAT
Sbjct: 61   GPLYCSPSSSMSLEES-TSTSQLEDSLLYSRAFWVSRHVIAWNVEVESGSCYLFASKMAT 120

Query: 266  LRVKGGVVEGYDAKIKLEKDYGPLTENVIKKFPHIQNYCPFTVPPESDVETLLKYQLAVA 325
            LRVK GVVEGYD KI LEKD   L ENVIKKFPHIQNYCPFTVPP SDVE LLK QLAVA
Sbjct: 121  LRVKDGVVEGYDVKITLEKDRSQLPENVIKKFPHIQNYCPFTVPPASDVEALLKCQLAVA 180

Query: 326  TFNSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYRDPV 385
            TF+SYG+C NITCLQLPGVLDDLFSYEGPLGAVYSKE VSLYLWAPTAQAV+AQI+RDPV
Sbjct: 181  TFDSYGECKNITCLQLPGVLDDLFSYEGPLGAVYSKEGVSLYLWAPTAQAVRAQIFRDPV 240

Query: 386  GGMPFEVIQLEELDGVWRTKGPRSWEGCYYEYEVTVYHPSTLQVEKCFTTDPYARGVSSD 445
            GGMPFEVI LEE+DGVWRTKGP+SWEGCYYEYEVTVYHPSTLQVEKCFTTDPY+RGVSSD
Sbjct: 241  GGMPFEVIPLEEVDGVWRTKGPKSWEGCYYEYEVTVYHPSTLQVEKCFTTDPYSRGVSSD 300

Query: 446  GRRTLFVDLGSDDLKPEGWDKLADKKPPVNSFSDISIYELHIRDFSISDQSVHPDLRGGY 505
            GRRTLFVDL SDDL P+GWDKLAD KPPV+SF+DISIYELH+RDFSISDQSVHPDLRGGY
Sbjct: 301  GRRTLFVDLCSDDLIPKGWDKLADDKPPVDSFTDISIYELHVRDFSISDQSVHPDLRGGY 360

Query: 506  MAFTLQDSAGITHLKKLSNAGLSHVHLLPTFQFAGVDDDKKKWKSLDTKPLENLPPDSAE 565
            MAFTLQDSAGI HLK LSNAGLSHVHLLPTF F GVDDDK KWK +DT+ LENLPPDSAE
Sbjct: 361  MAFTLQDSAGINHLKNLSNAGLSHVHLLPTFHFGGVDDDKTKWKFVDTELLENLPPDSAE 420

Query: 566  QQALIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLHVVLD 625
            QQ+LIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRL+EFRKMVQALNQIGL VVLD
Sbjct: 421  QQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLVEFRKMVQALNQIGLRVVLD 480

Query: 626  VVYNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDIL 685
            VVYNHLH HGP D NSVLDKIVPGYYLRRN DGFIENSTCVNNTASEHFMVERMIVDDIL
Sbjct: 481  VVYNHLHAHGPLDSNSVLDKIVPGYYLRRNPDGFIENSTCVNNTASEHFMVERMIVDDIL 540

Query: 686  HWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQSLTKEKNGVDGSSIYLYGEGWDFGEVAR 745
            HWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQ LTKEKNGVDGSSIY+YGEGWDFGEVA+
Sbjct: 541  HWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQGLTKEKNGVDGSSIYIYGEGWDFGEVAK 600

Query: 746  NGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDEV 805
            NGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDH HGT EV
Sbjct: 601  NGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHHHGTHEV 660

Query: 806  AEFMLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYASCPTETVNYAS 865
            AE MLAVSKDHI+VAMAANLRDY+LTNFEGKEVKGLEVLTHD SPVAYASCPTETVNY S
Sbjct: 661  AESMLAVSKDHIEVAMAANLRDYILTNFEGKEVKGLEVLTHDRSPVAYASCPTETVNYVS 720

Query: 866  AHDNETLFDVVSLKTPINITLNERCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDS 925
            AHDNETLFD+VSLKT  NIT+++RCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDS
Sbjct: 721  AHDNETLFDIVSLKTSRNITVDDRCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDS 780

Query: 926  YNSGDWFNRLDFTYMTNNWGVGLPPKEKNENNWPLIRPRLADPSFKPSKSHILAAVENFT 985
            YNSGDWFNRLDFTYMTNNWGVGLP KEKN+ NWPLI+PRLADPSFKPSKSHILAAVENFT
Sbjct: 781  YNSGDWFNRLDFTYMTNNWGVGLPLKEKNQYNWPLIKPRLADPSFKPSKSHILAAVENFT 840

Query: 986  NLLQIRYSSPLFRLKTSNAIQKRVRFHNTGASLIPGVIVMSIEDGHEGITGLSQLDPTYS 1045
            NLLQIRYSS LFRLKTS+AIQKRVRFHN+G SLIPG+IVMSIEDGHEGI GLSQLD TYS
Sbjct: 841  NLLQIRYSSRLFRLKTSHAIQKRVRFHNSGTSLIPGLIVMSIEDGHEGIPGLSQLDSTYS 900

Query: 1046 YIVVVVNARPTEISFPCPALRARTLQLHPIQLMSTDPVVKNSIYEPSTGCFMVPSRTTSV 1105
            YIVVV NARPTEISFPCPALRA+TLQLHPIQLMSTDP+VKNS YEPSTGCF+VP RTTSV
Sbjct: 901  YIVVVFNARPTEISFPCPALRAKTLQLHPIQLMSTDPIVKNSTYEPSTGCFVVPPRTTSV 960

Query: 1106 FVEPRIH 1113
            FVEPRIH
Sbjct: 961  FVEPRIH 965

BLAST of Lag0040467 vs. ExPASy Swiss-Prot
Match: Q8GTR4 (Pullulanase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PU1 PE=1 SV=2)

HSP 1 Score: 1331.6 bits (3445), Expect = 0.0e+00
Identity = 623/900 (69.22%), Postives = 750/900 (83.33%), Query Frame = 0

Query: 211  SSSASMSLEESAASTSQLEDSLLYSRAFWVSRYVIAWNVEVGNGSCYLFASKMATLRVKG 270
            S+S+ MSLE S+ + SQ  D L+YSRA+WV++ VIAWNV+VG GSCY +ASK A L    
Sbjct: 65   SASSPMSLEVSSPN-SQFLDCLIYSRAYWVTQGVIAWNVDVGEGSCYFYASKSAGLSFSE 124

Query: 271  GVVEGYDAKIKLEKDYGPLTENVIKKFPHIQNYCPFTVPPESDVETLLKYQLAVATFNSY 330
              ++GYD +IKLE + G L  +VI+KFPHI+NY  F VP + D+  L+K QLAV  F++ 
Sbjct: 125  DGIDGYDLRIKLEAESGSLPADVIEKFPHIRNYKSFKVPKDLDIRDLVKSQLAVVCFDAE 184

Query: 331  GQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYRDPVGGMPF 390
            G+    T LQLPGVLD+LFSY+GPLGA ++ E VSL+LWAPTAQAV   IY++P+   P 
Sbjct: 185  GRLIQGTGLQLPGVLDELFSYDGPLGAHFTPEGVSLHLWAPTAQAVSVCIYKNPLDKSPM 244

Query: 391  EVIQLEELDGVWRTKGPRSWEGCYYEYEVTVYHPSTLQVEKCFTTDPYARGVSSDGRRTL 450
            E+  L+E +GVW T+G  SW GCYY Y+V+VYHPST+++E C+  DPYARG+S+DGR+T 
Sbjct: 245  EICPLKEANGVWSTEGACSWGGCYYVYKVSVYHPSTMKLETCYANDPYARGLSADGRKTF 304

Query: 451  FVDLGSDDLKPEGWDKLADKKPPVNSFSDISIYELHIRDFSISDQSVHPDLRGGYMAFTL 510
             V+L SDDLKPEGWD LADKKP + SFSDISIYELH+RDFS +D++V P+ RGGY+AFT 
Sbjct: 305  LVNLDSDDLKPEGWDNLADKKPCLRSFSDISIYELHVRDFSANDETVEPENRGGYLAFTS 364

Query: 511  QDSAGITHLKKLSNAGLSHVHLLPTFQFAGVDDDKKKWKSLDTKPLENLPPDSAEQQALI 570
            +DSAG+ HL+KL +AGL+H+HLLPTFQF  VDD+K+ WKS+DT  LE L PDS E QA I
Sbjct: 365  KDSAGVKHLQKLVDAGLTHLHLLPTFQFGDVDDEKENWKSVDTSLLEGLRPDSTEAQARI 424

Query: 571  ADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLHVVLDVVYNH 630
             +IQN DGYNWGYNP+LWGVPKGSYASDP GPCR+IEFRKMVQALN  GL+VVLDVVYNH
Sbjct: 425  TEIQNDDGYNWGYNPVLWGVPKGSYASDPTGPCRIIEFRKMVQALNCTGLNVVLDVVYNH 484

Query: 631  LHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDILHWVVD 690
            LH  GP D  SVLDKIVPGYYLRRN+DGFIENSTCVNNTASEH+MV+R+I DD+L+WVV+
Sbjct: 485  LHASGPHDKESVLDKIVPGYYLRRNSDGFIENSTCVNNTASEHYMVDRLIRDDLLNWVVN 544

Query: 691  YKVDGFRFDLMGHLMKSTMLKAKDALQSLTKEKNGVDGSSIYLYGEGWDFGEVARNGRGV 750
            YKVDGFRFDLMGH+MK+T++ AK A+ SL KE +GVDGS IYLYGEGW+FGEVA NGRG+
Sbjct: 545  YKVDGFRFDLMGHIMKATIVNAKSAIGSLRKETDGVDGSRIYLYGEGWNFGEVAENGRGI 604

Query: 751  NASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDEVAEFML 810
            NASQFNL GTGIGSFNDR+RDA LGGSPFGHPLQQGF+TGLLL+PN HDHG++   E ML
Sbjct: 605  NASQFNLGGTGIGSFNDRIRDATLGGSPFGHPLQQGFITGLLLQPNAHDHGSEATQELML 664

Query: 811  AVSKDHIQVAMAANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYASCPTETVNYASAHDNE 870
            + +K+HIQ  MAANL+DY+LTN EGKEVKG EVL HD +PVAYAS PTET+NY SAHDNE
Sbjct: 665  STAKNHIQTGMAANLKDYMLTNHEGKEVKGSEVLMHDATPVAYASLPTETINYVSAHDNE 724

Query: 871  TLFDVVSLKTPINITLNERCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYNSGD 930
            TLFD++SLKTP+ I+++ERCRINHLA+S+IALSQGIPFFH GDE+LRSKS+DRDSYNSGD
Sbjct: 725  TLFDIISLKTPMEISVDERCRINHLASSMIALSQGIPFFHAGDEILRSKSLDRDSYNSGD 784

Query: 931  WFNRLDFTYMTNNWGVGLPPKEKNENNWPLIRPRLADPSFKPSKSHILAAVENFTNLLQI 990
            WFNRLDF+Y +NNWGVGLPPK KNE+NWPLI+PRL DPSFKP  SHI+A + NF +LL+I
Sbjct: 785  WFNRLDFSYSSNNWGVGLPPKGKNEHNWPLIKPRLQDPSFKPKSSHIVATLHNFLDLLRI 844

Query: 991  RYSSPLFRLKTSNAIQKRVRFHNTGASLIPGVIVMSIEDGHEGITGLSQLDPTYSYIVVV 1050
            RYSSPLFRL T+ AIQ+RVRFHNTG S IPG IVMSIEDGH GI  +SQ+DP YS IVV+
Sbjct: 845  RYSSPLFRLDTARAIQERVRFHNTGPSSIPGAIVMSIEDGHRGIPSVSQIDPIYSLIVVI 904

Query: 1051 VNARPTEISFPCPALRARTLQLHPIQLMSTDPVVKNSIYEPSTGCFMVPSRTTSVFVEPR 1110
             NARP+E S+P PAL+ R L+LHP+Q+MS D +VK S+Y+  +G F VP+RTT+VFVE R
Sbjct: 905  FNARPSEFSYPSPALKDRKLELHPVQVMSADEIVKKSVYDSFSGGFTVPARTTTVFVESR 963

BLAST of Lag0040467 vs. ExPASy Swiss-Prot
Match: P07206 (Pullulanase OS=Klebsiella pneumoniae OX=573 GN=pulA PE=1 SV=2)

HSP 1 Score: 544.3 bits (1401), Expect = 3.3e-153
Identity = 327/944 (34.64%), Postives = 505/944 (53.50%), Query Frame = 0

Query: 213  SASMSLEESAASTSQLEDSLLYSRAFWVSRYVIAWNVEVGNGSCYLFASKMATLRVKGGV 272
            + + ++ +S A   +    +  + A WV +  + W          L+ S  + +   G  
Sbjct: 162  AGNSAVYDSRADAFRAAFGVALAEAHWVDKNTLLWPGGQDKPIVRLYYSHSSKVAADG-- 221

Query: 273  VEGYDAKIKLEKDYGPLTENVIKKFPHIQNYCPFTVPPESDVETLLKYQLAVATFNSYGQ 332
             EG      L+     +++ V  +FPH+ +Y  F +P  ++V+ LL+ +         G 
Sbjct: 222  -EGKFTDRYLKLTPTTVSQQVSMRFPHLSSYAAFKLPDNANVDELLQGETVAIAAAEDGI 281

Query: 333  CNNITCLQLPGVLDDLFSYEG---PLGAVYSKEAVSLYLWAPTAQAVQAQIY---RDPVG 392
              + T +Q  GVLDD ++        GA  +   V+  +WAPTAQ V   +Y   +  +G
Sbjct: 282  LISATQVQTAGVLDDAYAEAAEALSYGAQLADGGVTFRVWAPTAQQVDVVVYSADKKVIG 341

Query: 393  GMPFEVIQLEELDGVWRTKGPRSWEGCYYEYEVTVYHPSTLQVEKCFTTDPYARGVSSDG 452
              P   +  +   G W  +G    +G +Y Y +TVYHP + +VE+   TDPYA  +S++ 
Sbjct: 342  SHP---MTRDSASGAWSWQGGSDLKGAFYRYAMTVYHPQSRKVEQYEVTDPYAHSLSTNS 401

Query: 453  RRTLFVDLGSDDLKPEGWDKLA--DKKPPVNSFSDISIYELHIRDFSISDQSVHPDLRGG 512
              +  VDL    LKP+GWD L     +      + ++I+E HIRD S  DQ+V  +LRG 
Sbjct: 402  EYSQVVDLNDSALKPDGWDNLTMPHAQKTKADLAKMTIHESHIRDLSAWDQTVPAELRGK 461

Query: 513  YMAFTLQDSAGITHLKKLSNAGLSHVHLLPTF---------------------------- 572
            Y+A T  DS  + HLK LS +G++HV LLP F                            
Sbjct: 462  YLALTAGDSNMVQHLKTLSASGVTHVELLPVFDLATVNEFSDKVADIQQPFSRLCEVNSA 521

Query: 573  ----QFAGVDDDKKKWKSLDTKPLENLPPDSAEQQALIADIQNSDGYNWGYNPILWGVPK 632
                +FAG  D     + +  +  ++   D+ + QAL   +  +D YNWGY+P  + VP+
Sbjct: 522  VKSSEFAGYCDSGSTVEEVLNQLKQSDSQDNPQVQALNTLVAQTDSYNWGYDPFHYTVPE 581

Query: 633  GSYASDPNGPCRLIEFRKMVQALNQ-IGLHVVLDVVYNHLHGHGPFDPNSVLDKIVPGYY 692
            GSYA+DP G  R+ EFR M+QA+ Q +G++V++DVVYNH +  GP D  SVLDKIVP YY
Sbjct: 582  GSYATDPEGTTRIKEFRTMIQAIKQDLGMNVIMDVVYNHTNAAGPTDRTSVLDKIVPWYY 641

Query: 693  LRRN-TDGFIENSTCVNNTASEHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMKSTML 752
             R N T G +E++TC +++A EH M  ++I D +  W  DYK+DGFRFDLMG+  K+ +L
Sbjct: 642  QRLNETTGSVESATCCSDSAPEHRMFAKLIADSLAVWTTDYKIDGFRFDLMGYHPKAQIL 701

Query: 753  KAKDALQSLTKEKNGVDGSSIYLYGEGWDFGEVARNGRGVNASQFNLFGTGIGSFNDRVR 812
             A + +++L  +        IY +GEGWD  +   + R   ASQ NL GTGIG+F+DR+R
Sbjct: 702  SAWERIKALNPD--------IYFFGEGWDSNQ---SDRFEIASQINLKGTGIGTFSDRLR 761

Query: 813  DAILGGSPF--GHPLQ--QGFVTGLLLEPNDHDHGTDEVAEFMLAVSKDHIQVAMAANLR 872
            D++ GG PF  G  L+  QG  +G  + PN+    +D+    +     D  ++ MA NL 
Sbjct: 762  DSVRGGGPFDSGDALRQNQGIGSGAGVLPNELASLSDDQVRHL----ADLTRLGMAGNLA 821

Query: 873  DYVLTNFEGKEVKGLEVLTHDGSPVAYASCPTETVNYASAHDNETLFDVVSLKTPINITL 932
            D+V+ + +G   KG E+  ++G+P  YA+ PTE VNY S HDN+TL+D++S K      L
Sbjct: 822  DFVMIDKDGAAKKGSEI-DYNGAPGGYAADPTEVVNYVSKHDNQTLWDMISYKASQEADL 881

Query: 933  NERCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGV 992
              R R+  ++ + + L QGI F   G ELLRSKS  RDSY+SGDWFNR+D++   NN+ V
Sbjct: 882  ATRVRMQAVSLATVMLGQGIAFDQQGSELLRSKSFTRDSYDSGDWFNRVDYSLQDNNYNV 941

Query: 993  GLPPKEKNENNWPLIRPRLADPSFKPSKSHILAAVENFTNLLQIRYSSPLFRLKTSNAIQ 1052
            G+P    + +N+ +I  R+ +    P ++ +      +  L ++R SSPLF L   +A+ 
Sbjct: 942  GMPRISDDGSNYEVI-TRVKEMVATPGEAELKQMTAFYQELTELRKSSPLFTLGDGSAVM 1001

Query: 1053 KRVRFHNTGASLIPGVIVMSIEDGHEGITGLSQLDPTYSYIVVVVNARPTEISFPCPALR 1111
            KRV F NTG+    G++VM+++D   G+   + LD     +VV +NA P   S       
Sbjct: 1002 KRVDFRNTGSDQQAGLLVMTVDD---GMKAGASLDSRLDGLVVAINAAPE--SRTLNEFA 1061

BLAST of Lag0040467 vs. ExPASy Swiss-Prot
Match: P07811 (Pullulanase OS=Klebsiella aerogenes OX=548 GN=pulA PE=1 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 2.3e-146
Identity = 311/869 (35.79%), Postives = 467/869 (53.74%), Query Frame = 0

Query: 213  SASMSLEESAASTSQLEDSLLYSRAFWVSRYVIAWNVEVGNGSCYLFASKMATLRVKGGV 272
            + + ++ +S A   +    +  + A WV +  + W          L+ S  + +      
Sbjct: 173  AGNSAVYDSRADAFRAAFGVALADAHWVDKTTLLWPGGENKPIVRLYYSHSSKVAADSN- 232

Query: 273  VEGYDAKIKLEKDYGPLTENVIKKFPHIQNYCPFTVPPESDVETLLKYQLAVATFNSYGQ 332
             E  D  +KL      + + V  +FPH+ +Y  F +P + +V+ LL+            +
Sbjct: 233  GEFSDKYVKLTPT--TVNQQVSMRFPHLASYPAFKLPDDVNVDELLQ-----GDDGGIAE 292

Query: 333  CNNITCLQLPGV-----------LDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIY 392
             + I  L  PG              +  SY    GA  +   V+  +WAPTAQ V+  IY
Sbjct: 293  SDGILSLSHPGADRRRAGRYLCRRAEALSY----GAQLTDSGVTFRVWAPTAQQVELVIY 352

Query: 393  RDPVGGMPFEVIQLEELDGVWRTKGPRSWEGCYYEYEVTVYHPSTLQVEKCFTTDPYARG 452
                  +    +  +   G W  +G    +G +Y Y +TVYHP + +VE+   TDPYA  
Sbjct: 353  SADKKVIASHPMTRDSASGAWSWQGGSDLKGAFYRYAMTVYHPQSRKVEQYEVTDPYAHS 412

Query: 453  VSSDGRRTLFVDLGSDDLKPEGWDKLA--DKKPPVNSFSDISIYELHIRDFSISDQSVHP 512
            +S++   +  VDL    LKPEGWD L     +      + ++I+E HIRD S  DQ+V  
Sbjct: 413  LSTNSEYSQVVDLNDSALKPEGWDGLTMPHAQKTKADLAKMTIHESHIRDLSAWDQTVPA 472

Query: 513  DLRGGYMAFTLQDSAGITHLKKLSNAGLSHVHLLPTF----------------------- 572
            +LRG Y+A T Q+S  + HLK+LS +G++H+ LLP F                       
Sbjct: 473  ELRGKYLALTAQESNMVQHLKQLSASGVTHIELLPVFDLATVNEFSDKVADIQQPFSRLC 532

Query: 573  ---------QFAGVDDDKKKWKSLDTKPLENLPPDSAEQQALIADIQNSDGYNWGYNPIL 632
                     +FAG  D     + + T+  +N   D+ + QAL   +  +D YNWGY+P  
Sbjct: 533  EVNSAVKSSEFAGYCDSGSTVEEVLTQLKQNDSKDNPQVQALNTLVAQTDSYNWGYDPFH 592

Query: 633  WGVPKGSYASDPNGPCRLIEFRKMVQALNQ-IGLHVVLDVVYNHLHGHGPFDPNSVLDKI 692
            + VP+GSYA+DP G  R+ EFR M+QA+ Q +G++V++DVVYNH +  GP D  SVLDKI
Sbjct: 593  YTVPEGSYATDPEGTARIKEFRTMIQAIKQDLGMNVIMDVVYNHTNAAGPTDRTSVLDKI 652

Query: 693  VPGYYLRRN-TDGFIENSTCVNNTASEHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLM 752
            VP YY R N T G +E++TC +++A EH M  ++I D +  W  DYK+DGFRFDLMG+  
Sbjct: 653  VPWYYQRLNETTGSVESATCCSDSAPEHRMFAKLIADSLAVWTTDYKIDGFRFDLMGYHP 712

Query: 753  KSTMLKAKDALQSLTKEKNGVDGSSIYLYGEGWDFGEVARNGRGVNASQFNLFGTGIGSF 812
            K+ +L A + +++L  +        IY +GEGWD  +   + R   ASQ NL GTGIG+F
Sbjct: 713  KAQILSAWERIKALNPD--------IYFFGEGWDSNQ---SDRFEIASQINLKGTGIGTF 772

Query: 813  NDRVRDAILGGSPF--GHPLQ--QGFVTGLLLEPNDHDHGTDEVAEFMLAVSKDHIQVAM 872
            +DR+RDA+ GG PF  G  L+  QG  +G  + PN+    +D+ A  +     D  ++ M
Sbjct: 773  SDRLRDAVRGGGPFDSGDALRQNQGVGSGAGVLPNELTTLSDDQARHL----ADLTRLGM 832

Query: 873  AANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYASCPTETVNYASAHDNETLFDVVSLKTP 932
            A NL D+VL + +G   +G E+  ++G+P  YA+ PTE VNY S HDN+TL+D++S K  
Sbjct: 833  AGNLADFVLIDKDGAVKRGSEI-DYNGAPGGYAADPTEVVNYVSKHDNQTLWDMISYKAA 892

Query: 933  INITLNERCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYNSGDWFNRLDFTYMT 992
                L+ R R+  ++ + + L QGI F   G ELLRSKS  RDSY+SGDWFNR+D++   
Sbjct: 893  QEADLDTRVRMQAVSLATVMLGQGIAFDQQGSELLRSKSFTRDSYDSGDWFNRVDYSLQD 952

Query: 993  NNWGVGLPPKEKNENNWPLIRPRLADPSFKPSKSHILAAVENFTNLLQIRYSSPLFRLKT 1031
            NN+ VG+P    + +N+ +I  R+ D    P ++ +      +  L  +R SSPLF L  
Sbjct: 953  NNYNVGMPRSSDDGSNYDII-ARVKDAVATPGETELKQMTAFYQELTALRKSSPLFTLGD 1012

BLAST of Lag0040467 vs. ExPASy Swiss-Prot
Match: O33840 (Pullulanase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=pulA PE=1 SV=2)

HSP 1 Score: 273.5 bits (698), Expect = 1.1e-71
Identity = 189/606 (31.19%), Postives = 304/606 (50.17%), Query Frame = 0

Query: 344 VLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYRDPVGGMPFEVIQLE-ELDGVW 403
           +LDD + Y+G LGAVYS E     +W+P ++ V+  ++++     P++V+ +E + +GVW
Sbjct: 215 ILDDYY-YDGELGAVYSPEKTIFRVWSPVSKWVKVLLFKNGEDTEPYQVVNMEYKGNGVW 274

Query: 404 RTKGPRSWEGCYYEYEVTVYHPSTLQVEKCFTTDPYARGVSSDGRRTLFVDLGSDDLKPE 463
                   +G +Y Y++  Y           T DPY++ V ++ +++  V+L   +  PE
Sbjct: 275 EAVVEGDLDGVFYLYQLENYGKIRT------TVDPYSKAVYANSKKSAVVNLARTN--PE 334

Query: 464 GWDKLADKKPPVNSFSDISIYELHIRDFSISDQSVHPDLRGGYMAFTLQDSAG----ITH 523
           GW+   D+ P +  + D  IYE+HI D +  + S   + +G Y+  T +++ G     T 
Sbjct: 335 GWEN--DRGPKIEGYEDAIIYEIHIADITGLENSGVKN-KGLYLGLTEENTKGPGGVTTG 394

Query: 524 LKKLSNAGLSHVHLLPTFQFAGVDDDKKKWKSLDTKPLENLPPDSAEQQALIADIQNSDG 583
           L  L   G++HVH+LP F F   D+  K ++                             
Sbjct: 395 LSHLVELGVTHVHILPFFDFYTGDELDKDFEKY--------------------------- 454

Query: 584 YNWGYNPILWGVPKGSYASDPNGP-CRLIEFRKMVQALNQIGLHVVLDVVYNHLHGHGPF 643
           YNWGY+P L+ VP+G Y++DP  P  R+ E ++MV+AL++ G+ V++D+V+ H +G G  
Sbjct: 455 YNWGYDPYLFMVPEGRYSTDPKNPHTRIREVKEMVKALHKHGIGVIMDMVFPHTYGIGEL 514

Query: 644 DPNSVLDKIVPGYYLR-RNTDGFIENSTCVNNTASEHFMVERMIVDDILHWVVDYKVDGF 703
              S  D+ VP Y+ R   T  ++  S C N  ASE  M+ + IVD + +WV +Y +DGF
Sbjct: 515 ---SAFDQTVPYYFYRIDKTGAYLNESGCGNVIASERPMMRKFIVDTVTYWVKEYHIDGF 574

Query: 704 RFDLMGHLMKSTMLKAKDALQSLTKEKNGVDGSSIYLYGEGW-DFGEVARNGRGVNASQF 763
           RFD MG + K TML+ + AL  +          +I LYGE W  +G   R G+       
Sbjct: 575 RFDQMGLIDKKTMLEVERALHKI--------DPTIILYGEPWGGWGAPIRFGKS------ 634

Query: 764 NLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDEVAEFMLAVSKD 823
           ++ GT + +FND  RDAI  GS F +P  +GFV G         +G            + 
Sbjct: 635 DVAGTHVAAFNDEFRDAI-RGSVF-NPSVKGFVMG--------GYG-----------KET 694

Query: 824 HIQVAMAANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYASCPTETVNYASAHDNETLFDV 883
            I+  +  ++      N++GK +K            ++A  P ET+NYA+ HDN TL+D 
Sbjct: 695 KIKRGVVGSI------NYDGKLIK------------SFALDPEETINYAACHDNHTLWDK 725

Query: 884 VSLKTPIN----ITLNERCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYNSGDW 938
             L    +     T  E      LA +I+  SQG+PF H G +  R+K+ + +SYN+   
Sbjct: 755 NYLAAKADKKKEWTEEELKNAQKLAGAILLTSQGVPFLHGGQDFCRTKNFNDNSYNAPIS 725

BLAST of Lag0040467 vs. ExPASy Swiss-Prot
Match: C0SPA0 (Pullulanase OS=Bacillus subtilis (strain 168) OX=224308 GN=amyX PE=1 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 1.5e-70
Identity = 230/737 (31.21%), Postives = 336/737 (45.59%), Query Frame = 0

Query: 305 PFTVPPESDVETLLKYQLAVATFNSYGQCNNI-------TCLQLPGVL-----DDLFSYE 364
           P  V  E  +E   KY        ++G+ + +       T LQ+  V+     DD F Y+
Sbjct: 44  PLAVREEYSLEAKYKYVCVSDHPVTFGKIHCVRASSGHKTDLQIGAVIRTAAFDDEFYYD 103

Query: 365 GPLGAVYSKEAVSLYLWAPTAQAVQAQIYRDPVGGMPFEVIQLEELDGVWRTKGPRSWEG 424
           G LGAVY+ +     +WAP A +   ++      G  F++ +LE+  GV+         G
Sbjct: 104 GELGAVYTADHTVFKVWAPAATSAAVKLSHPNKSGRTFQMTRLEK--GVYAVTVTGDLHG 163

Query: 425 CYYEYEVTVYHPSTLQVEKCFTTDPYARGVSSDGRRTLFVDLGSDDLKPEGWDKLADKKP 484
             YEY   + + S    E   T D YA+ V+ +G + +        L+P+     A  KP
Sbjct: 164 --YEYLFCICNNS----EWMETVDQYAKAVTVNGEKGVV-------LRPDQMKWTAPLKP 223

Query: 485 ---PVNSFSDISIYELHIRDFSISDQSVHPDLRGGYMAFTLQD-------SAGITHLKKL 544
              PV    D  IYE H+RDFSI + S   + +G Y+A T  D       S+G+ ++K+L
Sbjct: 224 FSHPV----DAVIYETHLRDFSIHENSGMIN-KGKYLALTETDTQTANGSSSGLAYVKEL 283

Query: 545 SNAGLSHVHLLPTFQFAGVDDDKKKWKSLDTKPLENLPPDSAEQQALIADIQNSDGYNWG 604
              G++HV LLP   FAGVD++         KPL                    D YNWG
Sbjct: 284 ---GVTHVELLPVNDFAGVDEE---------KPL--------------------DAYNWG 343

Query: 605 YNPILWGVPKGSYASDPNGP-CRLIEFRKMVQALNQIGLHVVLDVVYNHLHGHGPFDPNS 664
           YNP+ +  P+GSYAS+P+ P  R  E ++M+  L+Q GL V+LDVV+NH++       NS
Sbjct: 344 YNPLHFFAPEGSYASNPHDPQTRKTELKQMINTLHQHGLRVILDVVFNHVYKR----ENS 403

Query: 665 VLDKIVPGYYLRRNTDGFIENSTCV-NNTASEHFMVERMIVDDILHWVVDYKVDGFRFDL 724
             +K VPGY+ R +  G   N T V N+ ASE  M  + I D +++W+ +Y VDGFRFDL
Sbjct: 404 PFEKTVPGYFFRHDECGMPSNGTGVGNDIASERRMARKFIADCVVYWLEEYNVDGFRFDL 463

Query: 725 MGHLMKSTMLKAKDALQSLTKEKNGVDGSSIYLYGEGWDFG-EVARNGRGVNASQFNLFG 784
           +G L   T+L  K   +  TK K G     I L+GEGWD    +    +   A+   +  
Sbjct: 464 LGILDIDTVLYMK---EKATKAKPG-----ILLFGEGWDLATPLPHEQKAALANAPRM-- 523

Query: 785 TGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDEVAEFMLAVSKDHIQV 844
            GIG FND  RDA+ G +   H    GF  G         +G    A             
Sbjct: 524 PGIGFFNDMFRDAVKGNT--FHLKATGFALG---------NGESAQA------------- 583

Query: 845 AMAANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYASCPTETVNYASAHDNETLFDVVSLK 904
                    V+    G    G + L    +P+     P++++NY  +HDN T +D +S  
Sbjct: 584 ---------VMHGIAGS--SGWKAL----APIVPE--PSQSINYVESHDNHTFWDKMSFA 643

Query: 905 TPINITLNERCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYNSGDWFNRLDFTY 964
            P      +R R   LA +II L+QG+PF H G E  R+K    +SY S D  N+LD   
Sbjct: 644 LPQENDSRKRSR-QRLAAAIILLAQGVPFIHSGQEFFRTKQGVENSYQSSDSINQLD--- 665

Query: 965 MTNNWGVGLPPKEKNENNWPLIRPRLADPSFK--------------PSKSHILA------ 996
               W      KE       LI  R A P+F+                K H++A      
Sbjct: 704 ----WDRRETFKEDVHYIRRLISLRKAHPAFRLRSAADIQRHLECLTLKEHLIAYRLYDL 665

BLAST of Lag0040467 vs. ExPASy TrEMBL
Match: A0A6J1G1P3 (pullulanase 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111449862 PE=4 SV=1)

HSP 1 Score: 1783.1 bits (4617), Expect = 0.0e+00
Identity = 871/967 (90.07%), Postives = 906/967 (93.69%), Query Frame = 0

Query: 146  MSLLFSSSLHLLPSSTPVTSFRYILPTRIAAFSSSPPPGGLFSAVAGTSPSRYPDDHFRG 205
            MS LFSSSLHLLP S P TSF + LP RIAA SSSP  GG+FS VA TS S YP  H R 
Sbjct: 1    MSFLFSSSLHLLPPSNPATSFSHHLPPRIAAVSSSPAVGGVFS-VAATSLSHYPHGHSRC 60

Query: 206  GPLYCSSSASMSLEESAASTSQLEDSLLYSRAFWVSRYVIAWNVEVGNGSCYLFASKMAT 265
            GPLYCSSSASMSLEES  + SQLEDSLLY+RAFWVSRYVIAWNVEVG+GSCYLF SKMAT
Sbjct: 61   GPLYCSSSASMSLEESTPA-SQLEDSLLYARAFWVSRYVIAWNVEVGSGSCYLFGSKMAT 120

Query: 266  LRVKGGVVEGYDAKIKLEKDYGPLTENVIKKFPHIQNYCPFTVPPESDVETLLKYQLAVA 325
            LRV  GVVEGYD KIKLEKD GPL ENVIKKFPHIQNYCPFTVP  S VE LLK QLAVA
Sbjct: 121  LRVNDGVVEGYDVKIKLEKDRGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVA 180

Query: 326  TFNSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYRDPV 385
            TFNSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIY++PV
Sbjct: 181  TFNSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYKEPV 240

Query: 386  GGMPFEVIQLEELDGVWRTKGPRSWEGCYYEYEVTVYHPSTLQVEKCFTTDPYARGVSSD 445
            GG PFEVIQLEELDGVWRTKGP+SWEGCYYEYEVTVYHPSTL+VEKCF+TDPY+RGVSSD
Sbjct: 241  GGRPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGVSSD 300

Query: 446  GRRTLFVDLGSDDLKPEGWDKLADKKPPVNSFSDISIYELHIRDFSISDQSVHPDLRGGY 505
            GRRTLFVDLGSDDLKPEGWDKLAD+KPP++SFSDISIYELH+RDFSISDQSVHPD+RGGY
Sbjct: 301  GRRTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPDMRGGY 360

Query: 506  MAFTLQDSAGITHLKKLSNAGLSHVHLLPTFQFAGVDDDKKKWKSLDTKPLENLPPDSAE 565
            M FTLQDS GITHLKKLSNAG+SHVHLLPTFQF GVDDDK KWKSLD + LE LPPDSAE
Sbjct: 361  MGFTLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAE 420

Query: 566  QQALIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLHVVLD 625
            QQ+LIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGL VVLD
Sbjct: 421  QQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLD 480

Query: 626  VVYNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDIL 685
            VVYNHLHG GPFDP+SVLDKIVPGYYLRRN DGFIENSTCVNNTASEHFMVERMIVDDIL
Sbjct: 481  VVYNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDIL 540

Query: 686  HWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQSLTKEKNGVDGSSIYLYGEGWDFGEVAR 745
            HWVVDYKVDGFRFDLMGHLM STMLKAK+ALQ LT+EKNGVDGSSIYLYGEGWDFGEVA+
Sbjct: 541  HWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWDFGEVAK 600

Query: 746  NGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDEV 805
            NGRGVNASQFNLFGTGIGSFNDRVRDA+LGGSPFGHPLQQGFVTGLLLEPNDHDHGTD+ 
Sbjct: 601  NGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGTDKD 660

Query: 806  AEFMLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYASCPTETVNYAS 865
            AEF LAVSKDHIQVAMAANL+DYVL NFEGKEVKG EV T+DGS VAYASCPTETVNY S
Sbjct: 661  AEFTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSVAYASCPTETVNYVS 720

Query: 866  AHDNETLFDVVSLKTPINITLNERCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDS 925
            AHDNETLFDVVSLKTP NIT+N+RCR+NHLATSIIALSQGIPFFHCGDELLRSKSMDRDS
Sbjct: 721  AHDNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDELLRSKSMDRDS 780

Query: 926  YNSGDWFNRLDFTYMTNNWGVGLPPKEKNENNWPLIRPRLADPSFKPSKSHILAAVENFT 985
            YNSGDWFNRLDFTYMTNNWGVGLPPKEKNE NWPLI+PRLADPSFKPSKSHILAAVENFT
Sbjct: 781  YNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFT 840

Query: 986  NLLQIRYSSPLFRLKTSNAIQKRVRFHNTGASLIPGVIVMSIEDGHEGITGLSQLDPTYS 1045
            NLLQIRYSSPLFRLKT+NAIQKRVRFHNTG SLIPGVIVMSIEDG EGI GL+QLDP YS
Sbjct: 841  NLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYS 900

Query: 1046 YIVVVVNARPTEISFPCPALRARTLQLHPIQLMSTDPVVKNSIYEPSTGCFMVPSRTTSV 1105
            YIVVVVNARP+EISFPCPALRARTLQLHPIQLMSTDP+VKNS YEPSTGCFMVPSRTTSV
Sbjct: 901  YIVVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSV 960

Query: 1106 FVEPRIH 1113
            FVE R H
Sbjct: 961  FVELRKH 965

BLAST of Lag0040467 vs. ExPASy TrEMBL
Match: A0A0A0KTI2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G622510 PE=4 SV=1)

HSP 1 Score: 1764.6 bits (4569), Expect = 0.0e+00
Identity = 861/967 (89.04%), Postives = 901/967 (93.17%), Query Frame = 0

Query: 146  MSLLFSSSLHLLPSSTPVTSFRYILPTRIAAFSSSPPPGGLFSAVAGTSPSRYPDDHFRG 205
            MSLLFSSSLH  P S   TS  Y L    AAFSSSPP        AGTS S     +FR 
Sbjct: 1    MSLLFSSSLHFFPPSAFPTSLCYQLHHPFAAFSSSPP-------AAGTSLSPGFRHNFRT 60

Query: 206  GPLYCSSSASMSLEESAASTSQLEDSLLYSRAFWVSRYVIAWNVEVGNGSCYLFASKMAT 265
            GPLYCS S+SMSLEES  STSQLEDSLLYSRAFWVS++VIAWNVEV +GSCYLFASKMAT
Sbjct: 61   GPLYCSPSSSMSLEES-TSTSQLEDSLLYSRAFWVSKHVIAWNVEVESGSCYLFASKMAT 120

Query: 266  LRVKGGVVEGYDAKIKLEKDYGPLTENVIKKFPHIQNYCPFTVPPESDVETLLKYQLAVA 325
            LRV+ GVVEGYD KI+LEKD   L ENVIKKFPHIQNYCPFTVPP SDVE LLK QLAVA
Sbjct: 121  LRVEDGVVEGYDVKIRLEKDRSQLPENVIKKFPHIQNYCPFTVPPASDVEALLKCQLAVA 180

Query: 326  TFNSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYRDPV 385
            TFNSYG+C NITCLQLPGVLDDLFSYEGPLGA+YSKEAVSLYLWAPTAQAV+AQI+RDPV
Sbjct: 181  TFNSYGECKNITCLQLPGVLDDLFSYEGPLGAIYSKEAVSLYLWAPTAQAVRAQIFRDPV 240

Query: 386  GGMPFEVIQLEELDGVWRTKGPRSWEGCYYEYEVTVYHPSTLQVEKCFTTDPYARGVSSD 445
            GGMPFEVI LEE+DG+WRTKGP+SW+GCYYEYEVTVYHPSTLQVEKCFTTDPY+RGVSSD
Sbjct: 241  GGMPFEVIPLEEVDGIWRTKGPKSWKGCYYEYEVTVYHPSTLQVEKCFTTDPYSRGVSSD 300

Query: 446  GRRTLFVDLGSDDLKPEGWDKLADKKPPVNSFSDISIYELHIRDFSISDQSVHPDLRGGY 505
            GRRTLFVDL SDDL P+GWDKLAD+KPPV+SFSDISIYELH+RDFSISDQSVHPDLRGGY
Sbjct: 301  GRRTLFVDLFSDDLIPKGWDKLADEKPPVDSFSDISIYELHVRDFSISDQSVHPDLRGGY 360

Query: 506  MAFTLQDSAGITHLKKLSNAGLSHVHLLPTFQFAGVDDDKKKWKSLDTKPLENLPPDSAE 565
            MAFTLQDSAGI HLKKLSNAGLSHVHLLPTFQF GVDDDK KWK +DT+ LENLPPDSAE
Sbjct: 361  MAFTLQDSAGINHLKKLSNAGLSHVHLLPTFQFGGVDDDKTKWKFVDTELLENLPPDSAE 420

Query: 566  QQALIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLHVVLD 625
            QQ+LIADIQNSDGYNWGYNPI+WGVPKGSYASD NGPCRL+EFRKMVQALNQIGL VVLD
Sbjct: 421  QQSLIADIQNSDGYNWGYNPIMWGVPKGSYASDSNGPCRLVEFRKMVQALNQIGLRVVLD 480

Query: 626  VVYNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDIL 685
            VVYNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDIL
Sbjct: 481  VVYNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDIL 540

Query: 686  HWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQSLTKEKNGVDGSSIYLYGEGWDFGEVAR 745
            HWVVDYKVDGFRFDLMGHLMKSTMLKAKDAL+ LTKEKNGVDGSSIY+YGEGWDFGEVA+
Sbjct: 541  HWVVDYKVDGFRFDLMGHLMKSTMLKAKDALRGLTKEKNGVDGSSIYIYGEGWDFGEVAK 600

Query: 746  NGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDEV 805
            NGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGT EV
Sbjct: 601  NGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTHEV 660

Query: 806  AEFMLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYASCPTETVNYAS 865
            AE MLAVSKDHI+VAMAANLRDY+LTNFEGKEVKGLEVLTHD SPVAYASCPTETVNY S
Sbjct: 661  AESMLAVSKDHIEVAMAANLRDYILTNFEGKEVKGLEVLTHDRSPVAYASCPTETVNYVS 720

Query: 866  AHDNETLFDVVSLKTPINITLNERCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDS 925
            AHDNETLFD+VSLKTP NIT+++RCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDS
Sbjct: 721  AHDNETLFDIVSLKTPRNITVDDRCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDS 780

Query: 926  YNSGDWFNRLDFTYMTNNWGVGLPPKEKNENNWPLIRPRLADPSFKPSKSHILAAVENFT 985
            YNSGDWFNRLDFTYMTNNWGVGLP KEKN+ NWPLI+PRLADPSFKPSKSHILAAVENFT
Sbjct: 781  YNSGDWFNRLDFTYMTNNWGVGLPLKEKNQYNWPLIKPRLADPSFKPSKSHILAAVENFT 840

Query: 986  NLLQIRYSSPLFRLKTSNAIQKRVRFHNTGASLIPGVIVMSIEDGHEGITGLSQLDPTYS 1045
            NLLQIRYSSPLFRLKTSNAIQKRVRFHN+G SLIPG+IVMSIEDGH GI GLSQLD TYS
Sbjct: 841  NLLQIRYSSPLFRLKTSNAIQKRVRFHNSGTSLIPGLIVMSIEDGHNGIPGLSQLDSTYS 900

Query: 1046 YIVVVVNARPTEISFPCPALRARTLQLHPIQLMSTDPVVKNSIYEPSTGCFMVPSRTTSV 1105
            YIVVVVNARPTEISFPCPALRA+TLQLHPIQLMSTDPVVKNS YEPSTGCFMVP RTTSV
Sbjct: 901  YIVVVVNARPTEISFPCPALRAKTLQLHPIQLMSTDPVVKNSTYEPSTGCFMVPPRTTSV 959

Query: 1106 FVEPRIH 1113
            FVEPR+H
Sbjct: 961  FVEPRMH 959

BLAST of Lag0040467 vs. ExPASy TrEMBL
Match: A0A5A7SZ60 (Pullulanase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G007110 PE=4 SV=1)

HSP 1 Score: 1759.6 bits (4556), Expect = 0.0e+00
Identity = 861/967 (89.04%), Postives = 896/967 (92.66%), Query Frame = 0

Query: 146  MSLLFSSSLHLLPSSTPVTSFRYILPTRIAAFSSSPPPGGLFSAVAGTSPSRYPDDHFRG 205
            MSLLFSSSLH  PS    TS RY L  R AA SSSPP  GLFSA AGTS S     +FR 
Sbjct: 1    MSLLFSSSLHFFPSLASPTSLRYQLHPRFAASSSSPPAAGLFSA-AGTSLSHGSHHNFRT 60

Query: 206  GPLYCSSSASMSLEESAASTSQLEDSLLYSRAFWVSRYVIAWNVEVGNGSCYLFASKMAT 265
            GPLYCS S+SMSLEES  STSQLEDSLLYSRAFWVSR+VIAWNVEV +GSCYLFASKMAT
Sbjct: 61   GPLYCSPSSSMSLEES-TSTSQLEDSLLYSRAFWVSRHVIAWNVEVESGSCYLFASKMAT 120

Query: 266  LRVKGGVVEGYDAKIKLEKDYGPLTENVIKKFPHIQNYCPFTVPPESDVETLLKYQLAVA 325
            LRVK GVVEGYD KI LEKD   L ENVIKKFPHIQNYCPFTVPP SDVE LLK QLAVA
Sbjct: 121  LRVKDGVVEGYDVKITLEKDRSQLPENVIKKFPHIQNYCPFTVPPASDVEALLKCQLAVA 180

Query: 326  TFNSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYRDPV 385
            TF+SYG+C NITCLQLPGVLDDLFSYEGPLGAVYSKE VSLYLWAPTAQAV+AQI+RDPV
Sbjct: 181  TFDSYGECKNITCLQLPGVLDDLFSYEGPLGAVYSKEGVSLYLWAPTAQAVRAQIFRDPV 240

Query: 386  GGMPFEVIQLEELDGVWRTKGPRSWEGCYYEYEVTVYHPSTLQVEKCFTTDPYARGVSSD 445
            GGMPFEVI LEE+DGVWRTKGP+SWEGCYYEYEVTVYHPSTLQVEKCFTTDPY+RGVSSD
Sbjct: 241  GGMPFEVIPLEEVDGVWRTKGPKSWEGCYYEYEVTVYHPSTLQVEKCFTTDPYSRGVSSD 300

Query: 446  GRRTLFVDLGSDDLKPEGWDKLADKKPPVNSFSDISIYELHIRDFSISDQSVHPDLRGGY 505
            GRRTLFVDL SDDL P+GWDKLAD KPPV+SF+DISIYELH+RDFSISDQSVHPDLRGGY
Sbjct: 301  GRRTLFVDLCSDDLIPKGWDKLADDKPPVDSFTDISIYELHVRDFSISDQSVHPDLRGGY 360

Query: 506  MAFTLQDSAGITHLKKLSNAGLSHVHLLPTFQFAGVDDDKKKWKSLDTKPLENLPPDSAE 565
            MAFTLQDSAGI HLK LSNAGLSHVHLLPTF F GVDDDK KWK +DT+ LENLPPDSAE
Sbjct: 361  MAFTLQDSAGINHLKNLSNAGLSHVHLLPTFHFGGVDDDKTKWKFVDTELLENLPPDSAE 420

Query: 566  QQALIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLHVVLD 625
            QQ+LIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRL+EFRKMVQALNQIGL VVLD
Sbjct: 421  QQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLVEFRKMVQALNQIGLRVVLD 480

Query: 626  VVYNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDIL 685
            VVYNHLH HGP D NSVLDKIVPGYYLRRN DGFIENSTCVNNTASEHFMVERMIVDDIL
Sbjct: 481  VVYNHLHAHGPLDSNSVLDKIVPGYYLRRNPDGFIENSTCVNNTASEHFMVERMIVDDIL 540

Query: 686  HWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQSLTKEKNGVDGSSIYLYGEGWDFGEVAR 745
            HWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQ LTKEKNGVDGSSIY+YGEGWDFGEVA+
Sbjct: 541  HWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQGLTKEKNGVDGSSIYIYGEGWDFGEVAK 600

Query: 746  NGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDEV 805
            NGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDH HGT EV
Sbjct: 601  NGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHHHGTHEV 660

Query: 806  AEFMLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYASCPTETVNYAS 865
            AE MLAVSKDHI+VAMAANLRDY+LTNFEGKEVKGLEVLTHD SPVAYASCPTETVNY S
Sbjct: 661  AESMLAVSKDHIEVAMAANLRDYILTNFEGKEVKGLEVLTHDRSPVAYASCPTETVNYVS 720

Query: 866  AHDNETLFDVVSLKTPINITLNERCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDS 925
            AHDNETLFD+VSLKT  NIT+++RCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDS
Sbjct: 721  AHDNETLFDIVSLKTSRNITVDDRCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDS 780

Query: 926  YNSGDWFNRLDFTYMTNNWGVGLPPKEKNENNWPLIRPRLADPSFKPSKSHILAAVENFT 985
            YNSGDWFNRLDFTYMTNNWGVGLP KEKN+ NWPLI+PRLADPSFKPSKSHILAAVENFT
Sbjct: 781  YNSGDWFNRLDFTYMTNNWGVGLPLKEKNQYNWPLIKPRLADPSFKPSKSHILAAVENFT 840

Query: 986  NLLQIRYSSPLFRLKTSNAIQKRVRFHNTGASLIPGVIVMSIEDGHEGITGLSQLDPTYS 1045
            NLLQIRYSS LFRLKTS+AIQKRVRFHN+G SLIPG+IVMSIEDGHEGI GLSQLD TYS
Sbjct: 841  NLLQIRYSSRLFRLKTSHAIQKRVRFHNSGTSLIPGLIVMSIEDGHEGIPGLSQLDSTYS 900

Query: 1046 YIVVVVNARPTEISFPCPALRARTLQLHPIQLMSTDPVVKNSIYEPSTGCFMVPSRTTSV 1105
            YIVVV NARPTEISFPCPALRA+TLQLHPIQLMSTDP+VKNS YEPSTGCF+VP RTTSV
Sbjct: 901  YIVVVFNARPTEISFPCPALRAKTLQLHPIQLMSTDPIVKNSTYEPSTGCFVVPPRTTSV 960

Query: 1106 FVEPRIH 1113
            FVEPRIH
Sbjct: 961  FVEPRIH 965

BLAST of Lag0040467 vs. ExPASy TrEMBL
Match: A0A1S3BGM5 (pullulanase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103489432 PE=4 SV=1)

HSP 1 Score: 1759.6 bits (4556), Expect = 0.0e+00
Identity = 861/967 (89.04%), Postives = 896/967 (92.66%), Query Frame = 0

Query: 146  MSLLFSSSLHLLPSSTPVTSFRYILPTRIAAFSSSPPPGGLFSAVAGTSPSRYPDDHFRG 205
            MSLLFSSSLH  PS    TS RY L  R AA SSSPP  GLFSA AGTS S     +FR 
Sbjct: 1    MSLLFSSSLHFFPSLASPTSLRYQLHPRFAASSSSPPAAGLFSA-AGTSLSHGSHHNFRT 60

Query: 206  GPLYCSSSASMSLEESAASTSQLEDSLLYSRAFWVSRYVIAWNVEVGNGSCYLFASKMAT 265
            GPLYCS S+SMSLEES  STSQLEDSLLYSRAFWVSR+VIAWNVEV +GSCYLFASKMAT
Sbjct: 61   GPLYCSPSSSMSLEES-TSTSQLEDSLLYSRAFWVSRHVIAWNVEVESGSCYLFASKMAT 120

Query: 266  LRVKGGVVEGYDAKIKLEKDYGPLTENVIKKFPHIQNYCPFTVPPESDVETLLKYQLAVA 325
            LRVK GVVEGYD KI LEKD   L ENVIKKFPHIQNYCPFTVPP SDVE LLK QLAVA
Sbjct: 121  LRVKDGVVEGYDVKITLEKDRSQLPENVIKKFPHIQNYCPFTVPPASDVEALLKCQLAVA 180

Query: 326  TFNSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYRDPV 385
            TF+SYG+C NITCLQLPGVLDDLFSYEGPLGAVYSKE VSLYLWAPTAQAV+AQI+RDPV
Sbjct: 181  TFDSYGECKNITCLQLPGVLDDLFSYEGPLGAVYSKEGVSLYLWAPTAQAVRAQIFRDPV 240

Query: 386  GGMPFEVIQLEELDGVWRTKGPRSWEGCYYEYEVTVYHPSTLQVEKCFTTDPYARGVSSD 445
            GGMPFEVI LEE+DGVWRTKGP+SWEGCYYEYEVTVYHPSTLQVEKCFTTDPY+RGVSSD
Sbjct: 241  GGMPFEVIPLEEVDGVWRTKGPKSWEGCYYEYEVTVYHPSTLQVEKCFTTDPYSRGVSSD 300

Query: 446  GRRTLFVDLGSDDLKPEGWDKLADKKPPVNSFSDISIYELHIRDFSISDQSVHPDLRGGY 505
            GRRTLFVDL SDDL P+GWDKLAD KPPV+SF+DISIYELH+RDFSISDQSVHPDLRGGY
Sbjct: 301  GRRTLFVDLCSDDLIPKGWDKLADDKPPVDSFTDISIYELHVRDFSISDQSVHPDLRGGY 360

Query: 506  MAFTLQDSAGITHLKKLSNAGLSHVHLLPTFQFAGVDDDKKKWKSLDTKPLENLPPDSAE 565
            MAFTLQDSAGI HLK LSNAGLSHVHLLPTF F GVDDDK KWK +DT+ LENLPPDSAE
Sbjct: 361  MAFTLQDSAGINHLKNLSNAGLSHVHLLPTFHFGGVDDDKTKWKFVDTELLENLPPDSAE 420

Query: 566  QQALIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLHVVLD 625
            QQ+LIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRL+EFRKMVQALNQIGL VVLD
Sbjct: 421  QQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLVEFRKMVQALNQIGLRVVLD 480

Query: 626  VVYNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDIL 685
            VVYNHLH HGP D NSVLDKIVPGYYLRRN DGFIENSTCVNNTASEHFMVERMIVDDIL
Sbjct: 481  VVYNHLHAHGPLDSNSVLDKIVPGYYLRRNPDGFIENSTCVNNTASEHFMVERMIVDDIL 540

Query: 686  HWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQSLTKEKNGVDGSSIYLYGEGWDFGEVAR 745
            HWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQ LTKEKNGVDGSSIY+YGEGWDFGEVA+
Sbjct: 541  HWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQGLTKEKNGVDGSSIYIYGEGWDFGEVAK 600

Query: 746  NGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDEV 805
            NGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDH HGT EV
Sbjct: 601  NGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHHHGTHEV 660

Query: 806  AEFMLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYASCPTETVNYAS 865
            AE MLAVSKDHI+VAMAANLRDY+LTNFEGKEVKGLEVLTHD SPVAYASCPTETVNY S
Sbjct: 661  AESMLAVSKDHIEVAMAANLRDYILTNFEGKEVKGLEVLTHDRSPVAYASCPTETVNYVS 720

Query: 866  AHDNETLFDVVSLKTPINITLNERCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDS 925
            AHDNETLFD+VSLKT  NIT+++RCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDS
Sbjct: 721  AHDNETLFDIVSLKTSRNITVDDRCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDS 780

Query: 926  YNSGDWFNRLDFTYMTNNWGVGLPPKEKNENNWPLIRPRLADPSFKPSKSHILAAVENFT 985
            YNSGDWFNRLDFTYMTNNWGVGLP KEKN+ NWPLI+PRLADPSFKPSKSHILAAVENFT
Sbjct: 781  YNSGDWFNRLDFTYMTNNWGVGLPLKEKNQYNWPLIKPRLADPSFKPSKSHILAAVENFT 840

Query: 986  NLLQIRYSSPLFRLKTSNAIQKRVRFHNTGASLIPGVIVMSIEDGHEGITGLSQLDPTYS 1045
            NLLQIRYSS LFRLKTS+AIQKRVRFHN+G SLIPG+IVMSIEDGHEGI GLSQLD TYS
Sbjct: 841  NLLQIRYSSRLFRLKTSHAIQKRVRFHNSGTSLIPGLIVMSIEDGHEGIPGLSQLDSTYS 900

Query: 1046 YIVVVVNARPTEISFPCPALRARTLQLHPIQLMSTDPVVKNSIYEPSTGCFMVPSRTTSV 1105
            YIVVV NARPTEISFPCPALRA+TLQLHPIQLMSTDP+VKNS YEPSTGCF+VP RTTSV
Sbjct: 901  YIVVVFNARPTEISFPCPALRAKTLQLHPIQLMSTDPIVKNSTYEPSTGCFVVPPRTTSV 960

Query: 1106 FVEPRIH 1113
            FVEPRIH
Sbjct: 961  FVEPRIH 965

BLAST of Lag0040467 vs. ExPASy TrEMBL
Match: A0A6J1HT34 (LOW QUALITY PROTEIN: pullulanase 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111466529 PE=4 SV=1)

HSP 1 Score: 1671.8 bits (4328), Expect = 0.0e+00
Identity = 827/967 (85.52%), Postives = 862/967 (89.14%), Query Frame = 0

Query: 146  MSLLFSSSLHLLPSSTPVTSFRYILPTRIAAFSSSPPPGGLFSAVAGTSPSRYPDDHFRG 205
            MS LFSSSLHLLP S P TSFR+ LP RIAA  SSPP GG+FS VA TS S YPD H R 
Sbjct: 1    MSFLFSSSLHLLPPSNPATSFRHNLPPRIAAVPSSPPAGGIFS-VAATSLSHYPDGHSRC 60

Query: 206  GPLYCSSSASMSLEESAASTSQLEDSLLYSRAFWVSRYVIAWNVEVGNGSCYLFASKMAT 265
            GPLYCSSSASMS+EES  S SQLEDSLLY+RAFWVSRYVIAWNVEVG+GSCYLF SKMAT
Sbjct: 61   GPLYCSSSASMSVEES-TSASQLEDSLLYARAFWVSRYVIAWNVEVGSGSCYLFGSKMAT 120

Query: 266  LRVKGGVVEGYDAKIKLEKDYGPLTENVIKKFPHIQNYCPFTVPPESDVETLLKYQLAVA 325
            LRVK GVVEGYD KIKLEKD GPL ENVIKKFPHIQNYCPFTVP  S VE LLK QLAVA
Sbjct: 121  LRVKDGVVEGYDVKIKLEKDRGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVA 180

Query: 326  TFNSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYRDPV 385
            TFNSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIY++PV
Sbjct: 181  TFNSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYKEPV 240

Query: 386  GGMPFEVIQLEELDGVWRTKGPRSWEGCYYEYEVTVYHPSTLQVEKCFTTDPYARGVSSD 445
            GG PFEVIQLEELDGVWRTKGP+SW+GCYYEYEVTVYHPSTL+VEKCF+TDPY+RG+SSD
Sbjct: 241  GGRPFEVIQLEELDGVWRTKGPKSWDGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGLSSD 300

Query: 446  GRRTLFVDLGSDDLKPEGWDKLADKKPPVNSFSDISIYELHIRDFSISDQSVHPDLRGGY 505
            G RTLFVDLGSDDLKPEGWDKLAD+KPP++SFSDISIYELH+RDFSISDQSVHPDLRGGY
Sbjct: 301  GGRTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPDLRGGY 360

Query: 506  MAFTLQDSAGITHLKKLSNAGLSHVHLLPTFQFAGVDDDKKKWKSLDTKPLENLPPDSAE 565
            M F+LQDS GITHLKKLSNAG+SHVHLLPTFQF GVDDDK KWKSLD + LE LPPDSAE
Sbjct: 361  MGFSLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAE 420

Query: 566  QQALIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLHVVLD 625
            QQ+LIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGL VVLD
Sbjct: 421  QQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLD 480

Query: 626  VVYNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDIL 685
            VVYNHLHG GP DP+SVLDKIVPGYYLRRN DGFIENSTCVNNTASEHFMVERMIVDDIL
Sbjct: 481  VVYNHLHGCGPLDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDIL 540

Query: 686  HWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQSLTKEKNGVDGSSIYLYGEGWDFGEVAR 745
            HWVVDYKVDGFRFDLMGHLM STMLKAK+ALQ LT EKNGVDGSSIYLYGEGWDFGEVA+
Sbjct: 541  HWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTLEKNGVDGSSIYLYGEGWDFGEVAK 600

Query: 746  NGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDEV 805
            NGRGVNASQFNLFGTGIGSFNDRVRDA+LGGSPFGHPLQQGFVTGLLLEPNDHDHGTD+ 
Sbjct: 601  NGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGTDKD 660

Query: 806  AEFMLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYASCPTETVNYAS 865
            AEF LA+SKDHIQVAMAANLRDYVLTNFEGKEVKG E+ T+DGS VAY            
Sbjct: 661  AEFTLAMSKDHIQVAMAANLRDYVLTNFEGKEVKGSELRTYDGSSVAYXI---------- 720

Query: 866  AHDNETLFDVVSLKTPINITLNERCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDS 925
                 TLF                            L +GIPFFHCGDELLRSKSMDRDS
Sbjct: 721  -----TLF----------------------------LFKGIPFFHCGDELLRSKSMDRDS 780

Query: 926  YNSGDWFNRLDFTYMTNNWGVGLPPKEKNENNWPLIRPRLADPSFKPSKSHILAAVENFT 985
            YNSGDWFNRLDFTYMTNNWGVGLPPKEKNE NWPLI+PRLADPSFKPSKSHILAAVENFT
Sbjct: 781  YNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFT 840

Query: 986  NLLQIRYSSPLFRLKTSNAIQKRVRFHNTGASLIPGVIVMSIEDGHEGITGLSQLDPTYS 1045
            NLLQIRYSSPLFRLKT+NAIQKRVRFHNTG SLIPGVIVMSIEDG EGI GL+QLDP YS
Sbjct: 841  NLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYS 900

Query: 1046 YIVVVVNARPTEISFPCPALRARTLQLHPIQLMSTDPVVKNSIYEPSTGCFMVPSRTTSV 1105
            YIVVVVNARP+EISFPCP LRARTLQLHPIQLMSTDP+VKNS YEPSTGCFMVPSRTTSV
Sbjct: 901  YIVVVVNARPSEISFPCPVLRARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSV 922

Query: 1106 FVEPRIH 1113
            FVE R H
Sbjct: 961  FVELRXH 922

BLAST of Lag0040467 vs. TAIR 10
Match: AT5G04360.1 (limit dextrinase )

HSP 1 Score: 1331.6 bits (3445), Expect = 0.0e+00
Identity = 623/900 (69.22%), Postives = 750/900 (83.33%), Query Frame = 0

Query: 211  SSSASMSLEESAASTSQLEDSLLYSRAFWVSRYVIAWNVEVGNGSCYLFASKMATLRVKG 270
            S+S+ MSLE S+ + SQ  D L+YSRA+WV++ VIAWNV+VG GSCY +ASK A L    
Sbjct: 65   SASSPMSLEVSSPN-SQFLDCLIYSRAYWVTQGVIAWNVDVGEGSCYFYASKSAGLSFSE 124

Query: 271  GVVEGYDAKIKLEKDYGPLTENVIKKFPHIQNYCPFTVPPESDVETLLKYQLAVATFNSY 330
              ++GYD +IKLE + G L  +VI+KFPHI+NY  F VP + D+  L+K QLAV  F++ 
Sbjct: 125  DGIDGYDLRIKLEAESGSLPADVIEKFPHIRNYKSFKVPKDLDIRDLVKSQLAVVCFDAE 184

Query: 331  GQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYRDPVGGMPF 390
            G+    T LQLPGVLD+LFSY+GPLGA ++ E VSL+LWAPTAQAV   IY++P+   P 
Sbjct: 185  GRLIQGTGLQLPGVLDELFSYDGPLGAHFTPEGVSLHLWAPTAQAVSVCIYKNPLDKSPM 244

Query: 391  EVIQLEELDGVWRTKGPRSWEGCYYEYEVTVYHPSTLQVEKCFTTDPYARGVSSDGRRTL 450
            E+  L+E +GVW T+G  SW GCYY Y+V+VYHPST+++E C+  DPYARG+S+DGR+T 
Sbjct: 245  EICPLKEANGVWSTEGACSWGGCYYVYKVSVYHPSTMKLETCYANDPYARGLSADGRKTF 304

Query: 451  FVDLGSDDLKPEGWDKLADKKPPVNSFSDISIYELHIRDFSISDQSVHPDLRGGYMAFTL 510
             V+L SDDLKPEGWD LADKKP + SFSDISIYELH+RDFS +D++V P+ RGGY+AFT 
Sbjct: 305  LVNLDSDDLKPEGWDNLADKKPCLRSFSDISIYELHVRDFSANDETVEPENRGGYLAFTS 364

Query: 511  QDSAGITHLKKLSNAGLSHVHLLPTFQFAGVDDDKKKWKSLDTKPLENLPPDSAEQQALI 570
            +DSAG+ HL+KL +AGL+H+HLLPTFQF  VDD+K+ WKS+DT  LE L PDS E QA I
Sbjct: 365  KDSAGVKHLQKLVDAGLTHLHLLPTFQFGDVDDEKENWKSVDTSLLEGLRPDSTEAQARI 424

Query: 571  ADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLHVVLDVVYNH 630
             +IQN DGYNWGYNP+LWGVPKGSYASDP GPCR+IEFRKMVQALN  GL+VVLDVVYNH
Sbjct: 425  TEIQNDDGYNWGYNPVLWGVPKGSYASDPTGPCRIIEFRKMVQALNCTGLNVVLDVVYNH 484

Query: 631  LHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDILHWVVD 690
            LH  GP D  SVLDKIVPGYYLRRN+DGFIENSTCVNNTASEH+MV+R+I DD+L+WVV+
Sbjct: 485  LHASGPHDKESVLDKIVPGYYLRRNSDGFIENSTCVNNTASEHYMVDRLIRDDLLNWVVN 544

Query: 691  YKVDGFRFDLMGHLMKSTMLKAKDALQSLTKEKNGVDGSSIYLYGEGWDFGEVARNGRGV 750
            YKVDGFRFDLMGH+MK+T++ AK A+ SL KE +GVDGS IYLYGEGW+FGEVA NGRG+
Sbjct: 545  YKVDGFRFDLMGHIMKATIVNAKSAIGSLRKETDGVDGSRIYLYGEGWNFGEVAENGRGI 604

Query: 751  NASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDEVAEFML 810
            NASQFNL GTGIGSFNDR+RDA LGGSPFGHPLQQGF+TGLLL+PN HDHG++   E ML
Sbjct: 605  NASQFNLGGTGIGSFNDRIRDATLGGSPFGHPLQQGFITGLLLQPNAHDHGSEATQELML 664

Query: 811  AVSKDHIQVAMAANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYASCPTETVNYASAHDNE 870
            + +K+HIQ  MAANL+DY+LTN EGKEVKG EVL HD +PVAYAS PTET+NY SAHDNE
Sbjct: 665  STAKNHIQTGMAANLKDYMLTNHEGKEVKGSEVLMHDATPVAYASLPTETINYVSAHDNE 724

Query: 871  TLFDVVSLKTPINITLNERCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYNSGD 930
            TLFD++SLKTP+ I+++ERCRINHLA+S+IALSQGIPFFH GDE+LRSKS+DRDSYNSGD
Sbjct: 725  TLFDIISLKTPMEISVDERCRINHLASSMIALSQGIPFFHAGDEILRSKSLDRDSYNSGD 784

Query: 931  WFNRLDFTYMTNNWGVGLPPKEKNENNWPLIRPRLADPSFKPSKSHILAAVENFTNLLQI 990
            WFNRLDF+Y +NNWGVGLPPK KNE+NWPLI+PRL DPSFKP  SHI+A + NF +LL+I
Sbjct: 785  WFNRLDFSYSSNNWGVGLPPKGKNEHNWPLIKPRLQDPSFKPKSSHIVATLHNFLDLLRI 844

Query: 991  RYSSPLFRLKTSNAIQKRVRFHNTGASLIPGVIVMSIEDGHEGITGLSQLDPTYSYIVVV 1050
            RYSSPLFRL T+ AIQ+RVRFHNTG S IPG IVMSIEDGH GI  +SQ+DP YS IVV+
Sbjct: 845  RYSSPLFRLDTARAIQERVRFHNTGPSSIPGAIVMSIEDGHRGIPSVSQIDPIYSLIVVI 904

Query: 1051 VNARPTEISFPCPALRARTLQLHPIQLMSTDPVVKNSIYEPSTGCFMVPSRTTSVFVEPR 1110
             NARP+E S+P PAL+ R L+LHP+Q+MS D +VK S+Y+  +G F VP+RTT+VFVE R
Sbjct: 905  FNARPSEFSYPSPALKDRKLELHPVQVMSADEIVKKSVYDSFSGGFTVPARTTTVFVESR 963

BLAST of Lag0040467 vs. TAIR 10
Match: AT4G09020.1 (isoamylase 3 )

HSP 1 Score: 121.7 bits (304), Expect = 3.7e-27
Identity = 97/354 (27.40%), Postives = 160/354 (45.20%), Query Frame = 0

Query: 436 DPYARGVS-----SDGRRTLFVDLGSDDLKPEGWDKLADKKPPVNSFSDISIYELHIRDF 495
           DPYA+ V       D  +      G+ D +   +D   D K P     D+ IYE+++R F
Sbjct: 187 DPYAKLVKGHSSFGDSSQKFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAF 246

Query: 496 SISDQS-VHPDLRGGYMAFTLQDSAGITHLKKLSNAGLSHVHLLPTFQFAGVDDDKKKWK 555
           +  + S + P + G Y+ F       I  +  L + G++ V LLP F+F  ++  ++   
Sbjct: 247 TADESSGMDPAIGGSYLGF-------IEKIPHLQDLGINAVELLPVFEFDELELQRR--- 306

Query: 556 SLDTKPLENLPPDSAEQQALIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLI-EF 615
              + P +++                     WGY+ + +  P   YAS    P +   EF
Sbjct: 307 ---SNPRDHMV------------------NTWGYSTVNFFAPMSRYASGEGDPIKASKEF 366

Query: 616 RKMVQALNQIGLHVVLDVVYNHLHGHGPFDPNSVLDKIVPG--YYLRRNTDGFIENSTCV 675
           ++MV+AL+  G+ V+LDVVYNH +      P +   + +    YY+    +  +  S C 
Sbjct: 367 KEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDPNNQLLNFSGCG 426

Query: 676 NNTASEHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMKST---MLKAKDALQSLTKEK 735
           N     H +V  +I+D + HWV +Y VDGFRFDL   L ++T    L A   ++++ K+ 
Sbjct: 427 NTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDGSPLSAPPLIRAIAKDS 486

Query: 736 NGVDGSSIYLYGEGWDFGEVARNGRGVNASQFNLFGTGIGSFNDRVRDAILGGS 778
                S   +  E WD G +   G+  N   ++ +    G + D VR  I G S
Sbjct: 487 ---VLSRCKIIAEPWDCGGLYLVGKFPN---WDRWAEWNGMYRDDVRRFIKGDS 503

BLAST of Lag0040467 vs. TAIR 10
Match: AT2G39930.1 (isoamylase 1 )

HSP 1 Score: 96.3 bits (238), Expect = 1.7e-19
Identity = 135/538 (25.09%), Postives = 212/538 (39.41%), Query Frame = 0

Query: 436 DPYARGVSSDGRRTLFVDLGSDDLKPEGWDKLADKKPPVNS-------------FSDISI 495
           DPYA+ + S   R  F  LG DD     W ++A   P                   D+ I
Sbjct: 167 DPYAKAIIS---RDEFGVLGPDD---NCWPQMACMVPTREEEFDWEGDMHLKLPQKDLVI 226

Query: 496 YELHIRDFSISDQSVHPDLRGGYMAFTLQDSAGITHLKKLSNAGLSHVHLLPTFQFAGVD 555
           YE+H+R F+   +S   +  G Y       +  + HLK+L   G++ + L+P  +F    
Sbjct: 227 YEMHVRGFT-RHESSKIEFPGTYQGV----AEKLDHLKEL---GINCIELMPCHEF---- 286

Query: 556 DDKKKWKSLDTKPLENLPPDSAEQQALIADIQNSDGYNWGYNPILWGVPKGSY--ASDPN 615
            ++ ++ S +T               ++ D + +    WGY+ I +  P   Y  AS  N
Sbjct: 287 -NELEYYSYNT---------------ILGDHRVN---FWGYSTIGFFSPMIRYASASSNN 346

Query: 616 GPCRLI-EFRKMVQALNQIGLHVVLDVVYNHL---HGHGPFDPNSVLDKIVPGYYLRRNT 675
              R I EF+ +V+  ++ G+ V++DVV NH    +  GP      +D  V  YY+    
Sbjct: 347 FAGRAINEFKILVKEAHKRGIEVIMDVVLNHTAEGNEKGPIFSFRGVDNSV--YYMLAPK 406

Query: 676 DGFIENSTCVNNTASEHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMKSTMLKAKDAL 735
             F   S C N     H +V + I+D + +WV +  VDGFRFDL   + +S+ L   DA 
Sbjct: 407 GEFYNYSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLGSIMSRSSSL--WDAA 466

Query: 736 QSLTKEKNG--------------VDGSS-------IYLYGEGWDFGEVARNGRGVNASQF 795
                +  G              +D  S       + L  E WD G + + G       +
Sbjct: 467 NVYGADVEGDLLTTGTPISCPPVIDMISNDPILRGVKLIAEAWDAGGLYQVGM---FPHW 526

Query: 796 NLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDEVAEFMLAVSKD 855
            ++    G F D VR  I G   F       F   L   PN +  G           + D
Sbjct: 527 GIWSEWNGKFRDVVRQFIKGTDGF----SGAFAECLCGSPNLYQGGRKPWHSINFICAHD 586

Query: 856 HIQVAMAANLRDYVLTNFEGKEVKGLEVLTHDGSPVAYASCPTETVNYASAHDNETLFDV 915
              +A      D V  N +     G E   +DG          E  NY+     E  F  
Sbjct: 587 GFTLA------DLVTYNNKNNLANGEE--NNDG----------ENHNYSWNCGEEGDFAS 631

Query: 916 VSLKTPINITLNERCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYNSGDWFN 934
           +S+K      L +R   N   +  + +SQG+P  + GDE   +K  + ++Y   ++ N
Sbjct: 647 ISVK-----RLRKRQMRNFFVS--LMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYMN 631